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Minami, Naojiro

Graduate School of Agriculture, Division of Applied Biosciences Professor

Minami, Naojiro
list
    Last Updated :2022/10/02

    Basic Information

    Faculty

    • 農学部

    Professional Memberships

    • 日本生殖医学会
    • 日本分子生物学会
    • Society for the Study of Reproduction
    • 日本繁殖生物学会
    • 日本畜産学会

    Academic Degree

    • 農学修士(京都大学)
    • 農学博士(京都大学)

    Academic Resume (Graduate Schools)

    • 京都大学, 大学院農学研究科修士課程畜産学専攻, 修了
    • 京都大学, 大学院農学研究科博士後期課程畜産学専攻, 修了

    Academic Resume (Undergraduate School/Majors)

    • 京都大学, 農学部畜産学科(転学部), 卒業

    High School

    • High School

      大分県立宇佐高等学校

    Research History

    • From Apr. 2018
      京都大学農学研究科教授
    • From May 2006, To Mar. 2018
      京都大学農学研究科准教授
    • From Feb. 1991, To Apr. 2006
      Kyoto University, Faculty of Agriculture
    • From Apr. 1989, To Jan. 1991
      日本学術振興会特別研究員

    Language of Instruction

    • English

    ID,URL

    researchmap URL

    list
      Last Updated :2022/10/02

      Research

      Research Topics, Overview of the research

      • Research Topics

        Studies on gene expression in mammalian oocytes and preimplantation embryos
      • Overview of the research

        Development in mammals starts with fertilization. Before fertilization gene expression is cmpletly silenced in both oocytes and spermatozoa. Several hours after fiertilization gene expression starts in zygotic genome in the mouse and the first gene expression after fertilization is called zygotic gene expression (ZGA). Until that moment development is controlled by the factors accumulated in oocytes. Several oocyte-specific genes are identified to function only after fertilization and these genes are called maternal effect genes.My research projects are to investigate how do the genes expressed oocyte-specifically or the genes expressed after fertilization function to controll preimplantation development and to clarify how does the gene expression at ZGA or after fertilization controlled epigenetically.

      Research Interests

      • 卵子形成
      • 卵子
      • 遺伝子発現
      • 発生
      • oogenesis
      • oocyte
      • gene expression
      • development
      • embryo

      Research Areas

      • Life sciences, Zoological sciences
      • Life sciences, Animal production science

      Papers

      • Chimeric PRMT6 protein produced by an endogenous retrovirus promoter regulates cell fate decision in mouse preimplantation embryos
        Shinnosuke Honda; Maho Hatamura; Yuri Kunimoto; Shuntaro Ikeda; Naojiro Minami
        24 Aug. 2022
      • GFP-Dcp1aトランスジェニックマウスを用いた生体内のProcessing bodiesの可視化
        塚本 智史; 伊林 恵美; 和田 彩子; 鬼頭 靖司; 小久保 年章; 原 太一; 佐藤 健; 南 直治郎
        日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, Dec. 2015
      • マウス受精卵のオートファジー活性と胚発生能との関係に関する研究
        塚本 智史; 原 太一; 山本 篤; 鬼頭 靖司; 南 直治郎; 久保田 俊郎; 佐藤 健; 小久保 年章
        The Journal of Reproduction and Development, Aug. 2014
      • EMBRYO CULTURE IN EXPLANTED OVIDUCTS IN MICE AND CATTLE
        Y HOSOI; N MINAMI; A IRITANI
        HORMONE RESEARCH, 1995, Peer-reviewed
      • Genome-wide profiling of histone H3K4me3 and H3K27me3 modifications in individual blastocysts by CUT&Tag without a solid support (NON-TiE-UP CUT&Tag)
        Kazuki Susami; Shuntaro Ikeda; Yoichiro Hoshino; Shinnosuke Honda; Naojiro Minami
        Scientific Reports, Dec. 2022
      • Comparative analysis of histone H3K27me3 modifications between blastocysts and somatic tissues in cattle
        Satomi Yamazaki; Shuntaro Ikeda; Naojiro Minami
        Animal Science Journal, Jan. 2022
      • piggyBacトランスポゾンを用いたナイーブ型ウシiPS細胞の樹立の試み
        川口 高正; 築山 智之; 南 直治郎; 山田 雅保; 松山 秀一; 木村 康二; 今井 裕
        日本繁殖生物学会 講演要旨集, 2013
      • Comparative analysis of histone H3K4me3 modifications between blastocysts and somatic tissues in cattle
        Mao Ishibashi; Shuntaro Ikeda; Naojiro Minami
        Scientific Reports, Dec. 2021
      • Comparative analysis of histone H3K4me3 modifications between early embryos and somatic tissues in cattle
        Mao Ishibashi; Shuntaro Ikeda; Naojiro Minami
        12 Nov. 2020, Last author
      • ウシ初期胚におけるヒストンメチル化修飾への外因性メチオニンの関与
        太田 愛里; 池田 俊太郎; 杉本 実紀; 南 直治郎; 太田 毅
        The Journal of Reproduction and Development, Sep. 2020
      • Embryonic MTHFR contributes to blastocyst development
        Hiroki Ishitani; Shuntaro Ikeda; Kai Egashira; Miki Sugimoto; Shinichi Kume; Naojiro Minami; Takeshi Ohta
        Journal of Assisted Reproduction and Genetics, Aug. 2020
      • メチレンテトラヒドロ葉酸還元酵素(MTHFR)の胚盤胞発生への寄与について
        石谷 洋希; 池田 俊太郎; 杉本 実紀; 南 直治郎; 太田 毅
        日本生殖医学会雑誌, Apr. 2020, Peer-reviewed
      • H4K20 monomethylation inhibition causes loss of genomic integrity in mouse preimplantation embryos
        Daiki SHIKATA; Takuto YAMAMOTO; Shinnosuke HONDA; Shuntaro IKEDA; Naojiro MINAMI
        Journal of Reproduction and Development, 2020, Peer-reviewed
      • Deletion of the PDZ-binding kinase (Pbk) gene does not affect male fertility in mice
        Yuka Miki; Lalitha Devi; Yuji Imai; Naojiro Minami; Tsuyoshi Koide; Sandeep Goel
        Reproduction, Fertility and Development, 2020, Peer-reviewed
      • Synthesis and maintenance of lipid droplets are essential for mouse preimplantation embryonic development
        Ryutaro Aizawa; Megumi Ibayashi; Takayuki Tatsumi; Atsushi Yamamoto; Toshiaki Kokubo; Naoyuki Miyasaka; Ken Sato; Shuntaro Ikeda; Naojiro Minami; Satoshi Tsukamoto
        Development, 15 Nov. 2019, Peer-reviewed
      • ウシ初期胚のヒストンメチル化におけるメチオニンアデノシルトランスフェラーゼの役割
        太田 愛里; 池田 俊太郎; 杉本 実紀; 南 直治郎; 太田 毅
        The Journal of Reproduction and Development, Sep. 2019, Peer-reviewed
      • Altered hormonal milieu and dysregulated protein expression can cause spermatogenic arrest in ectopic xenografted immature rat testis
        Goel S; Minami N
        Scientific Reports, 2019, Peer-reviewed
      • How does the promoter of an oocyte-specific gene function in male germ cells?
        J Reprod Dev
        J Reprod Dev, Nov. 2018, Peer-reviewed
      • Long-term culture of undifferentiated spermatogonia isolated from immature and adult bovine testes
        Suyatno; Yuka Kitamura; Shuntaro Ikeda; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        Molecular Reproduction and Development, 01 Mar. 2018, Peer-reviewed
      • Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse.
        Takayuki Tatsumi; Kaori Takayama; Shunsuke Ishii; Atsushi Yamamoto; Taichi Hara; Naojiro Minami; Naoyuki Miyasaka; Toshiro Kubota; Akira Matsuura; Eisuke Itakura; Satoshi Tsukamoto
        Development (Cambridge, England), 23 Feb. 2018, Peer-reviewed
      • CHD1 Controls Cell Lineage Specification Through Zygotic Genome Activation
        Shinnosuke Suzuki; Naojiro Minami
        Advances in Anatomy Embryology and Cell Biology, 2018, Peer-reviewed
      • Oocyte-specific gene Oog1 suppresses the expression of spermatogenesis-specific genes in oocytes.
        Honda S; Miki Y; Miyamoto Y; Kawahara Y; Tsukamoto S; Imai H; Minami N
        J Reprod Dev., 2018, Peer-reviewed
      • Immobilized pH in culture reveals an optimal condition for somatic cell reprogramming and differentiation of pluripotent stem cells
        Narae Kim; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        Reproductive Medicine and Biology, 01 Jan. 2017, Peer-reviewed
      • Derivation of Induced Trophoblast Cell Lines in Cattle by Doxycycline-Inducible piggyBac Vectors
        Takamasa Kawaguchi; Dooseon Cho; Masafumi Hayashi; Tomoyuki Tsukiyama; Koji Kimura; Shuichi Matsuyama; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        PLOS ONE, Dec. 2016, Peer-reviewed
      • Deficiencies in extrusion of the second polar body due to high calcium concentrations during in vitro fertilization in inbred C3H/He mice
        Yuki Ohta; Yoshikazu Nagao; Naojiro Minami; Satoshi Tsukamoto; Seiji Kito
        ZYGOTE, Aug. 2016, Peer-reviewed
      • Factors supporting long-term culture of bovine male germ cells
        Mahesh Sahare; Sung-Min Kim; Ayagi Otomo; Kana Komatsu; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        REPRODUCTION FERTILITY AND DEVELOPMENT, 2016, Peer-reviewed
      • Generation of Naive Bovine Induced Pluripotent Stem Cells Using PiggyBac Transposition of Doxycycline-Inducible Transcription Factors
        Takamasa Kawaguchi; Tomoyuki Tsukiyama; Koji Kimura; Shuichi Matsuyama; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        PLOS ONE, Aug. 2015, Peer-reviewed
      • Histone methyltransferase Smyd3 regulates early embryonic lineage commitment in mice
        Shinnosuke Suzuki; Yusuke Nozawa; Satoshi Tsukamoto; Takehito Kaneko; Hiroshi Imai; Naojiro Minami
        REPRODUCTION, Jul. 2015, Peer-reviewed
      • CHD1 acts via the Hmgpi pathway to regulate mouse early embryogenesis
        Shinnosuke Suzuki; Yusuke Nozawa; Satoshi Tsukamoto; Takehito Kaneko; Ichiro Manabe; Hiroshi Imai; Naojiro Minami
        DEVELOPMENT, Jul. 2015, Peer-reviewed
      • A hyperactive piggyBac transposon system is an easy-to-implement method for introducing foreign genes into mouse preimplantation embryos
        Shinnosuke Suzuki; Tomoyuki Tsukiyama; Takehito Kaneko; Hiroshi Imai; Naojiro Minami
        JOURNAL OF REPRODUCTION AND DEVELOPMENT, Jun. 2015, Peer-reviewed
      • The role of signaling pathways on proliferation and self-renewal of cultured bovine primitive germ cells
        Mahesh Sahare; Ayagi Otomo; Kana Komatsu; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        Reproductive Medicine and Biology, 01 Jan. 2015, Peer-reviewed
      • Fluorescence-based visualization of autophagic activity predicts mouse embryo viability
        Satoshi Tsukamoto; Taichi Hara; Atsushi Yamamoto; Seiji Kito; Naojiro Minami; Toshiro Kubota; Ken Sato; Toshiaki Kokubo
        SCIENTIFIC REPORTS, Mar. 2014, Peer-reviewed
      • Effects of extracellular matrices and lectin Dolichos biflorus agglutinin on cell adhesion and self-renewal of bovine gonocytes cultured in vitro
        Sung-Min Kim; Mayako Fujihara; Mahesh Sahare; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        REPRODUCTION FERTILITY AND DEVELOPMENT, 2014, Peer-reviewed
      • ING3 Is Essential for Asymmetric Cell Division during Mouse Oocyte Maturation
        Shinnosuke Suzuki; Yusuke Nozawa; Satoshi Tsukamoto; Takehito Kaneko; Hiroshi Imai; Naojiro Minami
        PLOS ONE, Sep. 2013, Peer-reviewed
      • The Promoter of the Oocyte-Specific Gene, Oog1, Functions in Both Male and Female Meiotic Germ Cells in Transgenic Mice
        Miya Ishida; Eriko Okazaki; Satoshi Tsukamoto; Koji Kimura; Akira Aizawa; Seiji Kito; Hiroshi Imai; Naojiro Minami
        PLOS ONE, Jul. 2013, Peer-reviewed
      • Functional analysis of lysosomes during mouse preimplantation embryo development
        Satoshi Tsukamoto; Taichi Hara; Atsushi Yamamoto; Yuki Ohta; Ayako Wada; Yuka Ishida; Seiji Kito; Tetsu Nishikawa; Naojiro Minami; Ken Sato; Toshiaki Kokubo
        Journal of Reproduction and Development, 2013, Peer-reviewed
      • Functional analysis of lysosomes during mouse preimplantation embryo development
        Satoshi Tsukamoto; Taichi Hara; Atsushi Yamamoto; Yuki Ohta; Ayako Wada; Yuka Ishida; Seiji Kito; Tetsu Nishikawa; Naojiro Minami; Ken Sato; Toshiaki Kokubo
        Journal of Reproduction and Development, 2013, Peer-reviewed
      • Effects of extracellular matrices and the lectin DBA on cell adhesion and self-renewal of bovine gonocytes cultured
        Kim SM; Fujihara M; Sahare M; Minami N; Yamada M; Imai H
        Reprod Fert Dev, doi: 10.1071/RD12214 (in press), 2013, Peer-reviewed
      • Functional analysis of lysosomes during mouse preimplantation embryo development
        Satoshi Tsukamoto; Taichi Hara; Atsushi Yamamoto; Yuki Ohta; Ayako Wada; Yuka Ishida; Seiji Kito; Tetsu Nishikawa; Naojiro Minami; Ken Sato; Toshiaki Kokubo
        Journal of Reproduction and Development, 2013, Peer-reviewed
      • マウス着床前胚におけるリソソームの機能解析
        塚本 智史; 山本 篤; 原 太一; 南 直治郎; 鬼頭 靖司; 小久保 年章
        The Journal of Reproduction and Development, Aug. 2012
      • Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible factors and an EOS reporter system
        Tomoyuki Tsukiyama; Ryota Asano; Takamasa Kawaguchi; Narae Kim; Masayasu Yamada; Naojiro Minami; Yasuhide Ohinata; Hiroshi Imai
        GENES TO CELLS, Jul. 2011, Peer-reviewed
      • Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible factors and an EOS reporter system
        Tomoyuki Tsukiyama; Ryota Asano; Takamasa Kawaguchi; Narae Kim; Masayasu Yamada; Naojiro Minami; Yasuhide Ohinata; Hiroshi Imai
        GENES TO CELLS, Jul. 2011, Peer-reviewed
      • Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible factors and an EOS reporter system
        Tomoyuki Tsukiyama; Ryota Asano; Takamasa Kawaguchi; Narae Kim; Masayasu Yamada; Naojiro Minami; Yasuhide Ohinata; Hiroshi Imai
        GENES TO CELLS, Jul. 2011, Peer-reviewed
      • Characterization and In Vitro Culture of Male Germ Cells from Developing Bovine Testis
        Mayako Fujihara; Sung-Min Kim; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        JOURNAL OF REPRODUCTION AND DEVELOPMENT, Jun. 2011, Peer-reviewed
      • Characterization and In Vitro Culture of Male Germ Cells from Developing Bovine Testis
        Mayako Fujihara; Sung-Min Kim; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        JOURNAL OF REPRODUCTION AND DEVELOPMENT, Jun. 2011, Peer-reviewed
      • Characterization and In Vitro Culture of Male Germ Cells from Developing Bovine Testis
        Mayako Fujihara; Sung-Min Kim; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        JOURNAL OF REPRODUCTION AND DEVELOPMENT, Jun. 2011, Peer-reviewed
      • Identification and characterization of an oocyte factor required for development of porcine nuclear transfer embryos
        K. Miyamoto; K. Nagai; N. Kitamura; T. Nishikawa; H. Ikegami; N. T. Binh; S. Tsukamoto; M. Matsumoto; T. Tsukiyama; N. Minami; M. Yamada; H. Ariga; M. Miyake; T. Kawarasaki; K. Matsumoto; H. Imai
        Proceedings of the National Academy of Sciences, 26 Apr. 2011
      • 無線ICタグを用いた凍結精液流通管理システムの開発と実証試験
        星野洋一郎; 南 直治郎; 今井 裕
        日本胚移植学雑誌 = Japanese journal of embryo transfer, 19 Jan. 2011, Peer-reviewed
      • Identification of reprogramming factors associated with a pluripotent potential of porcine and bovine spermatic stem cells in culture
        S. M. Kim; M. Fujihara; S. Goel; M. Sahare; N. Minami; M. Yamada; H. Imai
        16TH WORLD CONGRESS ON IN VITRO FERTILIZATION AND THE 5TH WORLD CONGRESS ON IN VITRO MATURATION, 2011, Peer-reviewed
      • Cell-Free Extracts from Mammalian Oocytes Partially Induce Nuclear Reprogramming in Somatic Cells
        Kei Miyamoto; Tomoyuki Tsukiyama; Yang Yang; Ning Li; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        BIOLOGY OF REPRODUCTION, May 2009, Peer-reviewed
      • Cell-Free Extracts from Mammalian Oocytes Partially Induce Nuclear Reprogramming in Somatic Cells
        Kei Miyamoto; Tomoyuki Tsukiyama; Yang Yang; Ning Li; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        BIOLOGY OF REPRODUCTION, May 2009, Peer-reviewed
      • Multipotential ability of primitive germ cells from neonatal pig testis cultured in vitro
        Sandeep Goel; Mayako Fujihara; Kazuo Tsuchiya; Yuji Takagi; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        REPRODUCTION FERTILITY AND DEVELOPMENT, 2009, Peer-reviewed
      • Multipotential ability of primitive germ cells from neonatal pig testis cultured in vitro
        Sandeep Goel; Mayako Fujihara; Kazuo Tsuchiya; Yuji Takagi; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        REPRODUCTION FERTILITY AND DEVELOPMENT, 2009, Peer-reviewed
      • Multipotential ability of primitive germ cells from neonatal pig testis cultured in vitro
        Sandeep Goel; Mayako Fujihara; Kazuo Tsuchiya; Yuji Takagi; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        REPRODUCTION FERTILITY AND DEVELOPMENT, 2009, Peer-reviewed
      • Reversible Membrane Permeabilization of Mammalian Cells Treated with Digitonin and Its Use for Inducing Nuclear Reprogramming by Xenopus Egg Extracts
        Kei Miyamoto; Teruyoshi Yamashita; Tomoyuki Tsukiyama; Naoya Kitamura; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        CLONING AND STEM CELLS, Dec. 2008, Peer-reviewed
      • Reversible Membrane Permeabilization of Mammalian Cells Treated with Digitonin and Its Use for Inducing Nuclear Reprogramming by Xenopus Egg Extracts
        Kei Miyamoto; Teruyoshi Yamashita; Tomoyuki Tsukiyama; Naoya Kitamura; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        CLONING AND STEM CELLS, Dec. 2008, Peer-reviewed
      • Expression of NANOG, but not POU5F1, points to the stem cell potential of primitive germ cells in neonatal pig testis
        Sandeep Goel; Mayako Fujihara; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        REPRODUCTION, Jun. 2008, Peer-reviewed
      • Expression of NANOG, but not POU5F1, points to the stem cell potential of primitive germ cells in neonatal pig testis
        Sandeep Goel; Mayako Fujihara; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        REPRODUCTION, Jun. 2008, Peer-reviewed
      • Cryopreservation in liquid nitrogen of gonocytes from neonatal porcine testes stored at 4°C
        Mayako Fujihara; Sandeep Goel; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        Reproductive Medicine and Biology, 2008, Peer-reviewed
      • Cryopreservation in liquid nitrogen of gonocytes from neonatal porcine testes stored at 4°C
        Mayako Fujihara; Sandeep Goel; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        Reproductive Medicine and Biology, 2008, Peer-reviewed
      • Identification and expression analysis of small RNAs during development
        Toshiaki Watanabe; Hiroshi Imai; Naojiro Minami
        Methods in Molecular Biology, 2008, Peer-reviewed
      • Cryopreservation in liquid nitrogen of gonocytes from neonatal porcine testes stored at 4°C
        Mayako Fujihara; Sandeep Goel; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        Reproductive Medicine and Biology, 2008, Peer-reviewed
      • Reprogramming events of mammalian somatic cells induced by Xenopus laevis egg extracts
        Kei Miyamoto; Tadashi Furusawa; Mari Ohnuki; Sandeep Goel; Tomoyuki Tokunaga; Naojiro Minami; Masayasu Yamada; Keita Ohsumi; Hiroshi Imai
        MOLECULAR REPRODUCTION AND DEVELOPMENT, Oct. 2007, Peer-reviewed
      • Reprogramming events of mammalian somatic cells induced by Xenopus laevis egg extracts
        Kei Miyamoto; Tadashi Furusawa; Mari Ohnuki; Sandeep Goel; Tomoyuki Tokunaga; Naojiro Minami; Masayasu Yamada; Keita Ohsumi; Hiroshi Imai
        MOLECULAR REPRODUCTION AND DEVELOPMENT, Oct. 2007, Peer-reviewed
      • Zygotic gene activation and maternal factors in mammals.
        Naojiro Minami; Toru Suzuki; Satoshi Tsukamoto
        The Journal of reproduction and development, Aug. 2007, Peer-reviewed
      • Zygotic gene activation and maternal factors in mammals.
        Naojiro Minami; Toru Suzuki; Satoshi Tsukamoto
        The Journal of reproduction and development, Aug. 2007, Peer-reviewed
      • Zygotic gene activation and maternal factors in mammals.
        Naojiro Minami; Toru Suzuki; Satoshi Tsukamoto
        The Journal of reproduction and development, Aug. 2007, Peer-reviewed
      • Identification, isolation, and in vitro culture of porcine gonocytes
        Sandeep Goel; Miki Sugimoto; Naojiro Minami; Masayasu Yamada; Shinichi Kume; Hiroshi Imai
        BIOLOGY OF REPRODUCTION, Jul. 2007, Peer-reviewed
      • Identification, isolation, and in vitro culture of porcine gonocytes
        S. Goel; M. Sugimoto; N. Minami; M. Yamada; S. Kume; H. Imai
        Biology of Reproduction, Jul. 2007, Peer-reviewed
      • Identification, isolation, and in vitro culture of porcine gonocytes
        Sandeep Goel; Miki Sugimoto; Naojiro Minami; Masayasu Yamada; Shinichi Kume; Hiroshi Imai
        BIOLOGY OF REPRODUCTION, Jul. 2007, Peer-reviewed
      • Comparison of the RNA polymerase I-, II- and III-dependent transcript levels between nuclear transfer and in vitro fertilized embryos at the blastocyst stage.
        Toru Suzuki; Naojiro Minami; Tomohiro Kono; Hiroshi Imai
        The Journal of reproduction and development, Jun. 2007, Peer-reviewed
      • Comparison of the RNA polymerase I-, II- and III-dependent transcript levels between nuclear transfer and in vitro fertilized embryos at the blastocyst stage.
        Toru Suzuki; Naojiro Minami; Tomohiro Kono; Hiroshi Imai
        The Journal of reproduction and development, Jun. 2007, Peer-reviewed
      • Comparison of the RNA polymerase I-, II- and III-dependent transcript levels between nuclear transfer and in vitro fertilized embryos at the blastocyst stage.
        Toru Suzuki; Naojiro Minami; Tomohiro Kono; Hiroshi Imai
        The Journal of reproduction and development, Jun. 2007, Peer-reviewed
      • Effects of synchronization of donor cell cycle on embryonic development and DNA synthesis in porcine nuclear transfer embryos
        Kei Miyamoto; Yoichiro Hoshino; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        JOURNAL OF REPRODUCTION AND DEVELOPMENT, Apr. 2007, Peer-reviewed
      • Effects of synchronization of donor cell cycle on embryonic development and DNA synthesis in porcine nuclear transfer embryos
        Kei Miyamoto; Yoichiro Hoshino; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        Journal of Reproduction and Development, Apr. 2007, Peer-reviewed
      • Effects of synchronization of donor cell cycle on embryonic development and DNA synthesis in porcine nuclear transfer embryos
        Kei Miyamoto; Yoichiro Hoshino; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        JOURNAL OF REPRODUCTION AND DEVELOPMENT, Apr. 2007, Peer-reviewed
      • Effect of protopanaxatriol saponin on spermatogenic stem cell survival in busulfan-treated male mice
        Minyoung Ji; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        Reproductive Medicine and Biology, 2007, Peer-reviewed
      • Effect of protopanaxatriol saponin on spermatogenic stem cell survival in busulfan-treated male mice
        M. Ji; N. Minami; M. Yamada; H. Imai
        Reproductive Medicine and Biology, 2007, Peer-reviewed
      • Analysis of Small RNA Profiles During Development
        Toshiaki Watanabe; Yasushi Totoki; Hiroyuki Sasaki; Naojiro Minami; Hiroshi Imai
        Methods in Enzymology, 2007, Peer-reviewed
      • Effect of protopanaxatriol saponin on spermatogenic stem cell survival in busulfan-treated male mice
        Minyoung Ji; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        Reproductive Medicine and Biology, 2007, Peer-reviewed
      • ANALYSIS OF SMALL RNA PROFILES DURING DEVELOPMENT
        Toshiaki Watanabe; Yasushi Totoki; Hiroyuki Sasaki; Naojiro Minami; Hiroshi Imai
        MICRORNA METHODS, 2007, Peer-reviewed
      • ウシ胚盤胞期胚のcDNAライブラリー構築法
        塚本智史; 今市寿史; 木村康二; 今井 裕; 南 直治郎
        日本胚移植学雑誌 = Japanese journal of embryo transfer, 22 Sep. 2006, Peer-reviewed
      • Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes
        Toshiaki Watanabe; Atsushi Takeda; Tomoyuki Tsukiyama; Kazuyuki Mise; Tetsuro Okuno; Hiroyuki Sasaki; Naojiro Minami; Hiroshi Imai
        GENES & DEVELOPMENT, Jul. 2006, Peer-reviewed
      • Identification and characterization of two novel classes of small RNAs in the mouse germline: Retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes
        Toshiaki Watanabe; Atsushi Takeda; Tomoyuki Tsukiyama; Kazuyuki Mise; Tetsuro Okuno; Hiroyuki Sasaki; Naojiro Minami; Hiroshi Imai
        Genes and Development, 01 Jul. 2006, Peer-reviewed
      • Identification and characterization of two novel classes of small RNAs in the mouse germline: Retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes
        Toshiaki Watanabe; Atsushi Takeda; Tomoyuki Tsukiyama; Kazuyuki Mise; Tetsuro Okuno; Hiroyuki Sasaki; Naojiro Minami; Hiroshi Imai
        Genes and Development, 01 Jul. 2006, Peer-reviewed
      • Oog1, an oocyte-specific protein, interacts with Ras and Ras-signaling proteins during early embryogenesis
        S Tsukamoto; R Ihara; A Aizawa; S Kishida; A Kikuchi; H Imai; N Minami
        BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, May 2006, Peer-reviewed
      • Oog1, an oocyte-specific protein, interacts with Ras and Ras-signaling proteins during early embryogenesis
        S Tsukamoto; R Ihara; A Aizawa; S Kishida; A Kikuchi; H Imai; N Minami
        BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, May 2006, Peer-reviewed
      • Oog1, an oocyte-specific protein, interacts with Ras and Ras-signaling proteins during early embryogenesis
        S Tsukamoto; R Ihara; A Aizawa; S Kishida; A Kikuchi; H Imai; N Minami
        BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, May 2006, Peer-reviewed
      • Role of oocyte-specific genes in the development of mammalian embryos
        N. Minami; S. Tsukamoto
        Reproductive Medicine and Biology, 2006, Peer-reviewed
      • Role of oocyte-specific genes in the development of mammalian embryos
        Naojiro Minami; Satoshi Tsukamoto
        Reproductive Medicine and Biology, 2006, Peer-reviewed
      • Zygotically activated genes are suppressed in mouse nuclear transferred embryos.
        Toru Suzuki; Naojiro Minami; Tomohiro Kono; Hiroshi Imai
        Cloning and stem cells, 2006, Peer-reviewed
      • Role of oocyte-specific genes in the development of mammalian embryos
        Naojiro Minami; Satoshi Tsukamoto
        Reproductive Medicine and Biology, 2006, Peer-reviewed
      • Zygotically activated genes are suppressed in mouse nuclear transferred embryos.
        Toru Suzuki; Naojiro Minami; Tomohiro Kono; Hiroshi Imai
        Cloning and stem cells, 2006, Peer-reviewed
      • Stage-specific expression of microRNAs during Xenopus development.
        Toshiaki Watanabe; Atsushi Takeda; Kazuyuki Mise; Tetsuro Okuno; Toru Suzuki; Naojiro Minami; Hiroshi Imai
        FEBS letters, 17 Jan. 2005, Peer-reviewed
      • Stage-specific expression of microRNAs during Xenopus development.
        Toshiaki Watanabe; Atsushi Takeda; Kazuyuki Mise; Tetsuro Okuno; Toru Suzuki; Naojiro Minami; Hiroshi Imai
        FEBS letters, 17 Jan. 2005, Peer-reviewed
      • Stage-specific expression of microRNAs during Xenopus development.
        Toshiaki Watanabe; Atsushi Takeda; Kazuyuki Mise; Tetsuro Okuno; Toru Suzuki; Naojiro Minami; Hiroshi Imai
        FEBS letters, 17 Jan. 2005, Peer-reviewed
      • Developmental competence of somatic cell nuclear transfer embryos reconstructed from oocytes matured in vitro with follicle shells in miniature pig
        Yoichiro Hoshino; Masaki Uchida; Yoshiki Shimatsu; Masashi Miyake; Yasumitsu Nagao; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        Cloning and Stem Cells, 2005, Peer-reviewed
      • Developmental competence of somatic cell nuclear transfer embryos reconstructed from oocytes matured in vitro with follicle shells in miniature pig
        Yoichiro Hoshino; Masaki Uchida; Yoshiki Shimatsu; Masashi Miyake; Yasumitsu Nagao; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        Cloning and Stem Cells, 2005, Peer-reviewed
      • Cyclin A2 is phosphorylated during the G2/M transition in mouse two-cell embryos
        A Ohashi; H Imai; N Minami
        MOLECULAR REPRODUCTION AND DEVELOPMENT, Dec. 2003, Peer-reviewed
      • Cyclin A2 is phosphorylated during the G2/M transition in mouse two-cell embryos
        A Ohashi; H Imai; N Minami
        MOLECULAR REPRODUCTION AND DEVELOPMENT, Dec. 2003, Peer-reviewed
      • Oogenesin is a novel mouse protein expressed in oocytes and early cleavage-stage embryos
        N Minami; A Aizawa; R Ihara; M Miyamoto; A Ohashi; H Imai
        BIOLOGY OF REPRODUCTION, Nov. 2003, Peer-reviewed
      • Oogenesin is a novel mouse protein expressed in oocytes and early cleavage-stage embryos
        N Minami; A Aizawa; R Ihara; M Miyamoto; A Ohashi; H Imai
        BIOLOGY OF REPRODUCTION, Nov. 2003, Peer-reviewed
      • MuERV-L is one of the earliest transcribed genes in mouse one-cell embryos
        D Kigami; N Minami; H Takayama; H Imai
        BIOLOGY OF REPRODUCTION, Feb. 2003, Peer-reviewed
      • MuERV-L is one of the earliest transcribed genes in mouse one-cell embryos
        D Kigami; N Minami; H Takayama; H Imai
        BIOLOGY OF REPRODUCTION, Feb. 2003, Peer-reviewed
      • Glucose-6-phosphate dehydrogenase activity of IVM/IVF/IVC bovine embryos is related to the sex and developmental competence under suboptimal gas condition
        Iwata H; Kimura K; Hashimoto S; Oota M; Tominaga T; Minami N
        Journal of Reproduction and Development, Oct. 2002
      • Dependence of DNA synthesis and in vitro development of bovine nuclear transfer embryos on the stage of the cell cycle of donor cells and recipient cytoplasts
        S Kurosaka; Y Nagao; N Minami; M Yamada; H Imai
        BIOLOGY OF REPRODUCTION, Aug. 2002, Peer-reviewed
      • Bovine immature oocytes acquire developmental competence during meiotic arrest in vitro
        S Hashimoto; N Minami; R Takakura; H Imai
        BIOLOGY OF REPRODUCTION, Jun. 2002, Peer-reviewed
      • Role of G6PD activity on sex ratio and developmental competence of bovine bnbryos under oxidative stress
        Hisataka Iwata; Koji Kimura; Syu Hashimoto; Maiko Ohta; Keiichirou Tominaga; Naojirou Minami
        Journal of Reproduction and Development, 2002, Peer-reviewed
      • Role of G6PD activity on sex ratio and developmental competence of bovine bnbryos under oxidative stress
        Hisataka Iwata; Koji Kimura; Syu Hashimoto; Maiko Ohta; Keiichirou Tominaga; Naojirou Minami
        Journal of Reproduction and Development, 2002, Peer-reviewed
      • Role of G6PD activity on sex ratio and developmental competence of bovine bnbryos under oxidative stress
        Hisataka Iwata; Koji Kimura; Syu Hashimoto; Maiko Ohta; Keiichirou Tominaga; Naojirou Minami
        Journal of Reproduction and Development, 2002, Peer-reviewed
      • Nuclear accumulation of cyclin B1 in mouse two-cell embryos is controlled by the activation of Cdc2
        A Ohashi; N Minami; H Imai
        BIOLOGY OF REPRODUCTION, Oct. 2001, Peer-reviewed
      • E-cadherin localization in mouse embryos cultured in an oviductal environment
        Naojiro Minami; Hiroshi Imai
        Journal of Mammalian Ova Research, 2001, Peer-reviewed
      • Postnatal weights of calves derived from IVM/IVF embryos developed under various oxygen concentrations.
        Iwata H; Minami N; Imai H
        Reprod. Fertil. Dev.,12, 391-396, 2001, Peer-reviewed
      • Analysis of gene expression in mouse 2-cell embryos using fluorescein differential display: Comparison of culture environments
        N Minami; K Sasaki; A Aizawa; M Miyamoto; H Imai
        BIOLOGY OF REPRODUCTION, Jan. 2001, Peer-reviewed
      • Low oxygen tension during in vitro maturation is beneficial for supporting the subsequent development of bovine cumulus-oocyte complexes
        S Hashimoto; N Minami; R Takakura; M Yamada; H Imai; N Kashima
        MOLECULAR REPRODUCTION AND DEVELOPMENT, Dec. 2000, Peer-reviewed
      • Low oxygen tension during in vitro maturation is beneficial for supporting the subsequent development of bovine cumulus-oocyte complexes
        S Hashimoto; N Minami; R Takakura; M Yamada; H Imai; N Kashima
        MOLECULAR REPRODUCTION AND DEVELOPMENT, Dec. 2000, Peer-reviewed
      • Excessive concentration of glucose during in vitro maturation impairs the developmental competence of bovine oocytes after in vitro fertilization: Relevance to intracellular reactive oxygen species and glutathione contents
        S Hashimoto; N Minami; M Yamada; H Imai
        MOLECULAR REPRODUCTION AND DEVELOPMENT, Aug. 2000, Peer-reviewed
      • Relationship between the responsiveness to multiple-ovulation treatment and the number of bovine oocytes collected by transvaginal follicle aspiration
        S Hashimoto; R Takakura; M Yoshinari; N Minami; M Yamada; H Imai; N Kashima
        JOURNAL OF VETERINARY MEDICAL SCIENCE, Jun. 2000, Peer-reviewed
      • Relationship between the responsiveness to multiple-ovulation treatment and the number of bovine oocytes collected by transvaginal follicle aspiration
        S Hashimoto; R Takakura; M Yoshinari; N Minami; M Yamada; H Imai; N Kashima
        JOURNAL OF VETERINARY MEDICAL SCIENCE, Jun. 2000, Peer-reviewed
      • Relationship between the responsiveness to multiple-ovulation treatment and the number of bovine oocytes collected by transvaginal follicle aspiration
        S Hashimoto; R Takakura; M Yoshinari; N Minami; M Yamada; H Imai; N Kashima
        JOURNAL OF VETERINARY MEDICAL SCIENCE, Jun. 2000, Peer-reviewed
      • Excessive concentration of glucose during in vitro maturation impairs the developmental competence of bovine oocytes after in vitro fertilization: Relevance to intracellular reactive oxygen species and glutathione contents
        Shu Hashimoto; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        Molecular Reproduction and Development, 2000, Peer-reviewed
      • Postnatal weight of calves derived from in vitro matured and in vitro fertilized embryos developed under various oxygen concentrations
        H Iwata; N Minami; H Imai
        REPRODUCTION FERTILITY AND DEVELOPMENT, 2000, Peer-reviewed
      • Ultrasound-guided follicle aspiration: Effect of the frequency of a linear transvaginal probe on the collection of bovine oocytes
        S Hashimoto; R Takakura; N Minami; M Yamada
        THERIOGENOLOGY, Jul. 1999, Peer-reviewed
      • Ultrasound-guided follicle aspiration: Effect of the frequency of a linear transvaginal probe on the collection of bovine oocytes
        S Hashimoto; R Takakura; N Minami; M Yamada
        THERIOGENOLOGY, Jul. 1999, Peer-reviewed
      • Ultrasound-guided follicle aspiration: The collection of bovine cumulus-oocyte complexes from ovaries of slaughtered or live cows
        S Hashimoto; R Takakura; M Kishi; T Sudo; N Minami; M Yamada
        THERIOGENOLOGY, Mar. 1999, Peer-reviewed
      • Ultrasound-guided follicle aspiration: The collection of bovine cumulus-oocyte complexes from ovaries of slaughtered or live cows
        S Hashimoto; R Takakura; M Kishi; T Sudo; N Minami; M Yamada
        THERIOGENOLOGY, Mar. 1999, Peer-reviewed
      • Allopurinol, an inhibitor of xanthine oxidase, improves the development of IVM/IVF bovine embryos (> 4 cell) in vitro under certain culture conditions
        H Iwata; S Akamatsu; N Minami; M Yamada
        THERIOGENOLOGY, Feb. 1999, Peer-reviewed
      • Allopurinol, an inhibitor of xanthine oxidase, improves the development of IVM/IVF bovine embryos (> 4 cell) in vitro under certain culture conditions
        H Iwata; S Akamatsu; N Minami; M Yamada
        THERIOGENOLOGY, Feb. 1999, Peer-reviewed
      • マウス8細胞期はいに導入されたES細胞の局在
        上川奈都子; 南直治郎; 山田雅保
        日本はい移植学雑誌, 16 Jan. 1999, Peer-reviewed
      • Gene expression in mouse 2-cell embryos developed in an oviductal environment in vitro.
        N Minami; K Sasaki; A Aizawa; M Miyamoto; A Ohashi; H Imai
        BIOLOGY OF REPRODUCTION, 1999, Peer-reviewed
      • Effect of an oviductal environment on the expression and cellular localization of cell cycle regulatory proteins in mouse 2-cell embryos.
        A Ohashi; N Minami; K Sasaki; M Miyamoto; M Yamada; H Imai
        BIOLOGY OF REPRODUCTION, 1999, Peer-reviewed
      • Effects of antioxidants on the development of bovine IVM/IVF embryos in various concentrations of glucose
        H Iwata; S Akamatsu; N Minami; M Yamada
        THERIOGENOLOGY, Aug. 1998, Peer-reviewed
      • Effects of antioxidants on the development of bovine IVM/IVF embryos in various concentrations of glucose
        H Iwata; S Akamatsu; N Minami; M Yamada
        THERIOGENOLOGY, Aug. 1998, Peer-reviewed
      • Effects of cumulus cell density during in vitro maturation on the developmental competence of bovine oocytes
        S. Hashimoto; K. Saeki; Y. Nagao; N. Minami; M. Yamada; K. Utsumi
        Theriogenology, Jun. 1998, Peer-reviewed
      • Effects of cumulus cell density during in vitro maturation on the developmental competence of bovine oocytes
        S Hashimoto; K Saeki; Y Nagao; N Minami; M Yamada; K Utsumi
        THERIOGENOLOGY, Jun. 1998, Peer-reviewed
      • Effets of Leukemia Inhibitory Factor, Stem Cell Factor, and Basic Fibroblast Growth Factor on the Proliferation of Bovine ICM Cells in Vitro
        Rangsun Parnpai; Masanori Fujikawa; Naojiro Minami; Masayasu Yamada; Kyozo Utsumi
        Journal of Mammalian Ova Research, 1998, Peer-reviewed
      • Erratum: Effects of cumulus cell density during in vitro maturation on the developmental competence of bovine oocytes (An International Journal of Animal Reproduction (1998) 49 (1451-1463))
        S. Hashimoto; K. Saeki; Y. Nagao; N. Minami; M. Yamada; K. Utsumi
        Theriogenology, 1998, Peer-reviewed
      • 斉一な牛群を用いて作製した体外受精・体外発育卵と体内受精・体内発育卵に由来する産子体重の比較
        岩田 尚孝; 赤松 真一; 南 直治郎
        日本繁殖生物学会誌,44/,33-38, 1998, Peer-reviewed
      • Evaluation of acrosomal status of bovine spermatozoa using concanavalin A lectin
        A Nishikimi; M Yamada; N Minami; K Utsumi
        THERIOGENOLOGY, Oct. 1997, Peer-reviewed
      • Evaluation of acrosomal status of bovine spermatozoa using concanavalin A lectin
        A Nishikimi; M Yamada; N Minami; K Utsumi
        THERIOGENOLOGY, Oct. 1997, Peer-reviewed
      • Follicular cells affect the fertilizability and developmental competency of bovine oocytes in vitro
        KS Kim; N Minami; M Yamada; K Utsumi
        REPRODUCTION FERTILITY AND DEVELOPMENT, 1997, Peer-reviewed
      • Follicular cells affect the fertilizability and developmental competency of bovine oocytes in vitro
        KS Kim; N Minami; M Yamada; K Utsumi
        REPRODUCTION FERTILITY AND DEVELOPMENT, 1997, Peer-reviewed
      • Follicular cells affect the fertilizability and developmental competency of bovine oocytes in vitro
        KS Kim; N Minami; M Yamada; K Utsumi
        REPRODUCTION FERTILITY AND DEVELOPMENT, 1997, Peer-reviewed
      • Early embryonic development under oviductal influence in vitro
        N Minami
        ANIMAL REPRODUCTION SCIENCE, Apr. 1996, Peer-reviewed
      • Early embryonic development under oviductal influence in vitro
        N. Minami
        Animal Reproduction Science, Apr. 1996, Peer-reviewed
      • Analysis of a nuclear localization signal of simian virus 40 major capsid protein Vp1
        N Ishii; N Minami; EY Chen; AL Medina; MM Chico; H Kasamatsu
        JOURNAL OF VIROLOGY, Feb. 1996, Peer-reviewed
      • Early embryonic development under oviductal influence in vitro
        Naojiro Minami
        Animal Reproduction Science, 1996, Peer-reviewed
      • NONSPECIES-SPECIFIC EFFECTS OF MOUSE OVIDUCTS ON THE DEVELOPMENT OF BOVINE IVM/IVF EMBRYOS BY A SERUM-FREE COCULTURE
        N MINAMI; H KATO; Y INOUE; M YAMADA; K UTSUMI; A IRITANI
        THERIOGENOLOGY, May 1994, Peer-reviewed
      • Effect of cell surface N-linked oligosaccharide chains on the compaction of preimplantation mouse embryos
        H.Hayashi; N.Minami; M.Yamada; K.Utsumi
        Nihon Seishokumeneki Gakkai Zassi, 1994
      • ホ乳動物初期胚の発生におよぼす卵管の影響
        南 直治郎
        受精着床学会誌,11/,26-27, 1994, Peer-reviewed
      • ROLE OF THE OVIDUCT IN THE DEVELOPMENT OF THE MOUSE EMBRYO
        N MINAMI; A IRITANI
        MOLECULAR REPRODUCTION AND DEVELOPMENT, Oct. 1993, Peer-reviewed
      • EFFECTS OF LOW-MOLECULAR-WEIGHT OVIDUCTAL FACTORS ON THE DEVELOPMENT OF MOUSE ONE-CELL EMBRYOS INVITRO
        N MINAMI; K UTSUMI; A IRITANI
        JOURNAL OF REPRODUCTION AND FERTILITY, Nov. 1992, Peer-reviewed
      • DEVELOPMENT OF HAMSTER 2-CELL EMBRYOS IN THE ISOLATED MOUSE OVIDUCT IN ORGAN-CULTURE SYSTEM
        N MINAMI; BD BAVISTER; A IRITANI
        GAMETE RESEARCH, Mar. 1988, Peer-reviewed
      • 耐凍剤および凍結融解法が-196℃に保存された家兎桑実胚の生存性に及ぼす影響
        南 直治郎
        家畜繁殖学雑誌, Mar. 1984, Peer-reviewed
      • PHOTO-VOLTAIC PROPERTIES OF PHTHALOCYANINE PARTICULATE FILMS AND EFFECTS OF PRETREATMENTS OF PIGMENT
        N MINAMI; K SASAKI
        KOBUNSHI RONBUNSHU, 1983, Peer-reviewed

      Misc.

      • 精子完成過程におけるオートファジーの生理機能の解析
        塚本智史; 久万亜紀子; 岸千絵子; PHAM NGUYEN Quy; 太田有紀; 西川哲; 岡崎絵里子; 南直治郎; 水島昇
        J Reprod Dev, 20 Aug. 2011
      • Effect of the cumulus cell density in the in vitro maturation of cattle ovum on the later-stage growth of embryo.
        HASHIMOTO NOTARU; SAEKI KAZUHIRO; NAGAO NORIKAZU; NAGAI MASAKI; KIN KOSHOKU; MINAMI NAOJIRO; YAMADA MASAYASU; UTSUMI KYOZO
        日本繁殖生物学会講演要旨, Oct. 1996
      • 無血清培養におけるウシ初期はいの発生におよぼすマウス卵管の影響
        加藤博己; 南直治郎; 山田雅保; 内海恭三
        日本畜産学会大会講演要旨, Mar. 1994
      • 無血清培地中で卵胞細胞がウシ未成熟卵胞卵子の成熟と体外受精後のはい発生に及ぼす影響
        加藤博己; 細井美彦; 南直治郎; 山田雅保; 内海恭三
        日本畜産学会大会講演要旨, Mar. 1993
      • Analysis of protein recognized by antibody produced using peptide containing transcription factor NF-.KAPPA.B nucleus conversion signal as immunogen and its localization in initial-stage embryo of mouse.
        錦見昭彦; 向井二郎; 西岡和歌子; 南直治郎; 山田雅保
        日本繁殖生物学会講演要旨, 1997
      • Expression of transcription factor NF-kB in mouse initial-stage embryo before implantation.
        向井二郎; 錦見昭彦; 南直治郎; 山田雅保
        日本繁殖生物学会講演要旨, 1997
      • Effect of nitrogen monoxide synthetase inhibitor on generation of initial-stage embryo of mouse.
        山田雅保; 錦見昭彦; 向井二郎; 南直治郎
        日本繁殖生物学会講演要旨, 1997
      • Effects of Follicular Cells and Conditioned Medium of Granulosa Cells on In Vitro Maturation and Fertilization of Bovine Oocytes and Subsequent Embryonic Development
        KIM K. S.; MINAMI N.; YAMADA M.; UTSUMI K.
        日本胚移植学雑誌 = Japanese journal of embryo transfer, 17 Sep. 1996
      • Gene Expression of Mouse Embryos Cultured in an Oviductal Environment by Differential Display
        SASAKI Kana; MINAMI Naojiro; OHASHI Akihiro; YAMADA Masayasu
        Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 01 Apr. 1998
      • Knockoutanalysis of oocyte-specific multi-copy gene, Oog1
        Yuka Miki; Satoshi Tsukamoto; Hiroshi Imai; Naojiro Minami
        51st Annual Meeting of the Society for the Study of Reproduction, 10 Jul. 2018, Peer-reviewed
      • Knockout analysisi of oocyte-specific multi-copy gene, Oog1.
        Miki Y; Tsukamoto S; Imai H; Minami N
        The 13rd Annual Conference of the Asian Reproductive Biotechnology Society, 03 May 2018, Peer-reviewed
      • Functional analysis of histone demethylase Kdm5b in mouse preimplantation development
        Mao Kurasaka; Takehito Kaneko; Shinnosuke Suzuki; Satoshi Tsukamoto; Hiroshi Imai; Naojiro Minami
        Symposium for the next generation of stem cell researchers (SYStem), 22 Feb. 2018, Peer-reviewed
      • Functional analysis of histone demethylase Kdm5b in mouse preimplantation development.
        Kurasaka M; Kaneko T; Suzuki S; Tsukamoto S; Imai H; Minami N
        4th World Congress of Reproductive Biology, 27 Sep. 2017, Peer-reviewed
      • The role of SMYD3 in methylation of H3K4 of Hmgpi promoter regions in mouse preimplantation embryos.
        Honda S; Suzuki S; Tsukamoto S; Kaneko T; Imai H; Minami N
        4th World Congress of Reproductive Biology, 27 Sep. 2017, Peer-reviewed
      • A long-term culture system supplemented with 6-bromoindirubin-3’-oxime (bio) maintains proliferation of germ cells from adult testis in cattle.
        Suyatno; Kitamura Y; Minami N; Yamada M; Imai H
        50th Annual Meeting of the Society for the Study of Reproduction, 13 Jul. 2017, Peer-reviewed
      • Methylation of H3K4 of Hmgpi promoter region by SMYD3 in mouse preimplantation embryos.
        Honda S; Suzuki S; Tsukamoto S; Kaneko T; Imai H; Minami N
        50th Annual Meeting of the Society for the Study of Reproduction, 13 Jul. 2017, Peer-reviewed
      • Improvement of long-term culture system for bovine spermatogonial stem cells.
        Suyatno; Kitamura Y; Minami N; Yamada M; Imai H
        The 7th Congress of the Asian Pacific Invitative on Reproduction, 30 Mar. 2017, Peer-reviewed
      • Induction of differentiation of bovine male germ cells in culture.
        Kitamura Y; Suyatno; Minami N; Yamada M; Imai H
        The 7th Congress of the Asian Pacific Invitative on Reproduction, 30 Mar. 2017, Peer-reviewed
      • Epigentic states of chicken fibroblasts are changed during reprogramming processes by pH in culture.
        Kim N; Minami N; Yamada M; Imai H
        18th International Congress on Animal Reproduction, 26 Jun. 2016, Peer-reviewed
      • BMP4 is involved in maintenance or derivation of bovine primordial germ cells from bovine induced pluripotent stem cells.
        Hayashi M; Cho D; Kawaguchi T; Minami N; Yamada M; Imai H
        18th International Congress on Animal Reproduction, 26 Jun. 2016, Peer-reviewed
      • HSP90 regulates the pluripotency-related pathways and supports normal preimplantation embryonic development in mice.
        Cho D; Phuong LQ; Minami N; Yamada M; Imai H
        18th International Congress on Animal Reproduction, 26 Jun. 2016, Peer-reviewed
      • ウス着床前の初期胚発生における熱ショックたんぱく質(HSP90)の役割
        趙 杜善; Q. P. Luong; 南直治郎; 山田雅保; 今井裕
        第4回関西生殖医学集談会第48回関西アンドロロジーカンファレンス合同研究会, Mar. 2016
      • xternal pH affects the timing of mouse somatic cell reprogramming.
        N. Kim; M. Yamada; N. Minami; H. Imai
        ISSCR 2015, Jun. 2015, Peer-reviewed
      • In vivo analysis of processing bodies using transgenic mice expressing GFP-Dcp1a
        Tsukamoto S; Hara T; Sato K; Minami N; Kiro S; Tsukamoto S
        15th International Congress of Radiation Research, May 2015, Peer-reviewed
      • The pH range of culture medium affects chicken somatic cell reprogramming.
        N. Kim; M. Yamada; N. Minami; H. Imai
        Keystone Symposia C9: Transcriptional and Epigenetic Influences on Stem Cell States,, Mar. 2015
      • The role of Oog1 in female meiotic germ cells: Activation of oocyte-specific genes and suppression of testis-associated genes.
        Miyamoto Y; Kawahara Y; Suzuki S; Ishida M; Tsukamoto S; Imai H; Minami N
        The 10th Annual Conference of the Asian Reproductive Biotechnology Society, Nov. 2014, Peer-reviewed
      • CHD1 is the zygotic determinant of Oct4 and Cdx2 expression via the Hmgpi pathway during preimplantation development.
        Suzuki, S; Nozawa Y; Tsukamoto S; Kaneko T; Imai H; Minami N
        2014 World Congress of Reproductive Biology, Sep. 2014, Peer-reviewed
      • Fluorescence-based visualization of autophagic activity predicts mouse embryo viability (vol 4, 4533, 2014)
        Satoshi Tsukamoto; Taichi Hara; Atsushi Yamamoto; Seiji Kito; Naojiro Minami; Toshiro Kubota; Ken Sato; Toshiaki Kokubo
        SCIENTIFIC REPORTS, Aug. 2014
      • piggyBac トランスホソンを用いたリフログラミンク因子導入によるウシ羊膜細胞からの栄養膜細胞株樹立の試み
        川口 高正; 築山 智之; 松山 秀一; 木村 康二; 南 直治郎; 山田 雅保; 今井 裕
        第107回日本繁殖生物学会大会, Aug. 2014
      • イエネコ体細胞を用いたリフロクラミンク誘導に関する研究
        古田 侑吾; 川口 高正; 南 直治郎; 山田 雅保; 稲葉 俊夫; 今井 裕
        第107回日本繁殖生物学会大会, Aug. 2014
      • Oog1 functions during meiosis of female germ cells through suppression of testis-associated genes and activation of oocyte-specific genes.
        Miyamoto Y; Kawahara Y; Suzuki S; Ishida M; Tsukamoto S; Imai H; Minami N
        The 47th Annual Meeting of the Society for the Study of Reproduction, Aug. 2014, Peer-reviewed
      • イエネコ体細胞を用いた人工多能性幹細胞の誘導
        古田侑吾; 川口高正; 南直治郎; 山田雅保; 稲葉俊夫; 今井裕
        平成25年度〔第63回〕関西畜産学会大会, 06 Sep. 2013, Peer-reviewed
      • Mitogen activated protein kinase (MAPK) signaling is indispensable for maintaining self renewal and proliferation of bovine male germ stem cells in culture.
        M. G. Sahare; A. Otomo; S.M. Kim; K. Komatsu; N. Minami; M. Yamada; H. Imai
        46th Annual Meeting of Society for the Study of Reproduction, 23 Jul. 2013, Peer-reviewed
      • The role of Ing3 during oocyte maturation in the mouse
        SUZUKI Shinnosuke; NOZAWA Yusuke; KANEKO Takehito; IMAI Hiroshi; MINAMI Naojiro
        Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 01 Apr. 2013
      • Long term self-renewal and proliferation of bovine male germ stem cells in vitro
        M Sahare; S-M Kim; A Otomo; K Koamatsu; N Minami; M Yamada; H Imai
        CiRA International Symposium 2013 ~Raising the next generation of stem cell research~, Kyoto, Japan, 11 Mar. 2013, Peer-reviewed
      • Generation of murine-ES-cell-like iPS cells in cattle using piggyBac transposition of doxycycline-inducible transcription factor
        T. Kawaguchi; T. Tsukiyama; N. Minami; M. Yamada; K. Kimura; H. Imai
        CiRA International Symposium 2013 ~Raising the next generation of stem cell research~, Kyoto, Japan, 11 Mar. 2013, Peer-reviewed
      • ウシにおけるマウスES細胞様iPS細胞の樹立の試み
        川口 高正; 南 直治郎; 山田 雅保; 木村 康二; 今井 裕
        第1回 関西生殖医学集談会, 02 Mar. 2013, Peer-reviewed
      • The roles of Hsp90 in reprogramming process in mouse preimplantation embryonic development
        Luong PQ; Matsumoto M; Suzuki S; Kitamura N; Miyamoto K; Minami N; Yamada M; Imai H
        CiRA International Symposium, 2013, Peer-reviewed
      • Ing3 has an important role during oocyte maturation in the mouse
        Suzuki S; Nozawa Y; Tsukamoto S; Kaneko T; Imai H; Minami N
        The 46th Annual Meeting of the Society for the Study of Reproduction, 2013, Peer-reviewed
      • Monitoring of autophagic activity in the developing mouse preimplantation embryo.
        Tsukamoto S; Hara T; Yamamoto A; Kito S; Minami N; Sato K; Kokubo T
        The 5th Europian Molecular Biology Organaization Meeting, 2013, Peer-reviewed
      • マウス初期胚におけるヒストンメチル化酵素Smyd3の役割について
        野澤佑介; 鈴木伸之介; 塚本智史; 金子武人; 今井 裕; 南 直治郎
        第105回日本繁殖生物学会、講演要旨集、j186, 07 Sep. 2012
      • The role of GalNAc glycan residues associated adhesion and self-renewal in cultured bovine gonocytes.
        Sung-Min Kim; Mayako Fujihara; Mahesh Sahare; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        The 17th International Congress on Animal Reproduction (ICAR). Vancouver, British Columbia, Canada, 29 Jul. 2012, Peer-reviewed
      • Transdifferentiation of bovine aminion cells into trophoblast-like cells using piggyBac transposition of doxycycline-inducible reprogramming factors
        T. Kawaguchi; T. Tsukiyama; N. Minami; M. Yamada; K. Kimura; H. Imai
        Satellite symposium to the International Congress on Animal Reproduction (ICAR), Jul. 2012, Peer-reviewed
      • Derivation of murine-ES-cell like iPS cells in canine under the EOS reporter system
        T. Kawaguchi; R. Asano; Tsukiyama. T; N. Minami; M. Yamada; S. Hatoya; K. Sugiura; T. Inaba; H. Imai
        International Society for Stem Cell Research (ISSCR), Jun. 2012, Peer-reviewed
      • TRACEABILITY SYSTEM FOR AN INDIVIDUAL FROZEN SEMEN STRAW BY A TINY RADIO FREQUENCY IDENTIFICATION CHIP
        Y. Hoshino; K. Mukojima; N. Minami; H. Imai
        REPRODUCTION FERTILITY AND DEVELOPMENT, 2012
      • The functional analysis of Chd1 in mouse oocyte maturation using lipid-siRNA complexes
        Suzuki S; Tsukamoto S; Ohota Y; Imai Hiroshi; Minami N
        Joint Meeting of the Brithish Society for Cell Biology, Developmental Biology and the Japanese Society for Developmental Biologists, 2012, Peer-reviewed
      • Effects of chromatin remodeling factor Chd1 on in vitro differentiation of mouse preimplantation embryos
        Suzuki S; Nozawa Y; Tsukamoto S; Kaneko T; Imai H; Minami N
        10th Annual Meetinf of International Socirety for Stem Cell Reseach, 2012, Peer-reviewed
      • Determination of distinct germ cell commitment by the specific affinity of the lectin dba during early development of gonocytes in neonatal mice testis
        Kim SM; Fujihara M; Goel S; Suzuki S; Minami N; Yamada M; Imai H
        International Congress of Animal Reproduction, 2012, Peer-reviewed
      • Oog1 promoter controls the meiosis-specific gene expression in germ cells
        Ishida M; Okazaki E; Tsukamoto S; Kimura K; Ohota Y; Kito S; Imai H; Minami N
        The 45th Annual Meeting of the Society for the Study of Reproduction, 2012, Peer-reviewed
      • Glycan epitopes involve in germ cell differentiation during early development of neonatal mice testis
        Kim SM; Fujihara M; Goel S; Suzuki S; Minami N; Yamada M; Imai H
        4th Congress of the Asia Pacific Initiaive on Reproduction, 2012, Peer-reviewed
      • Differential effects of extracellular matrix associated with cell adhesion and self-renewal of bovine gonocytes cultured in vitro.
        Sung-Min Kim; Mayako Fujihara; Mahesh Sahare; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        The 2nd World Congress on Reproductive Biology (WCRB), Cairns, QSL, Australia, 09 Oct. 2011, Peer-reviewed
      • Establishment of induced pluripotent stem cells in cattle using piggyBac transposition of doxycycline-inducible transcription factors
        T. Kawaguchi; T. Tsukiyama; K. Kimura; N. Minami; M. Yamada; H. Imai
        World Congress on Reproductive Biology (WCRB), Cairns, Australia, 09 Oct. 2011
      • マウス初期胚における黒町ん再構成タンパク質Chd1に関する研究
        鈴木伸之介; 野澤佑介; 塚本智史; 金子武人; 今井 裕; 南直治郎
        第105回日本繁殖生物学会、講演要旨集、j91, 06 Sep. 2011
      • Identification and characterization of an oocyte factor required for development of porcine nuclear transfer embryos
        Kei Miyamoto; Kouhei Nagai; Naoya Kitamura; Tomoaki Nishikawa; Haruka Ikegami; Nguyen T. Binh; Satoshi Tsukamoto; Mai Matsumoto; Tomoyuki Tsukiyama; Naojiro Minami; Masayasu Yamada; Hiroyoshi Ariga; Masashi Miyake; Tatsuo Kawarasaki; Kazuya Matsumoto; Hiroshi Imai
        PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Apr. 2011, Peer-reviewed
      • イヌにおける人工多能性幹細胞樹立の試み
        浅野良太; 築山智之; 南直治郎; 山田雅保; 今井裕; 鳩谷晋吾; 杉浦喜久弥; 稲葉俊夫
        第136回日本生殖医学会関西支部集談会, 05 Mar. 2011, Peer-reviewed
      • Identification of Oog1 Promoter Regions in Mouse Growing Oocytes
        Miya Ishida; Eriko Okazaki; Satoshi Tsukamoto; Koji Kimura; Yuki Ohta; Seiji Kito; Hiroshi Imai; Naojiro Minami
        BIOLOGY OF REPRODUCTION, Jul. 2011, Peer-reviewed
      • 様々な動物種におけるマウスES細胞様・ヒトES細胞様iPS細胞樹立の試み
        築山智之; 浅野良太; 南直治郎; 山田雅保; 今井 裕
        第9回日本再生医療学会、講演要旨集 P-045、広島国際会議場, Mar. 2010
      • Comparative Changes on Stem Cell Potential of Bovine Male Germ Cells During Testicular Development from Neonatal to Adult
        Mayako Fujihara; Sung Min Kim; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        BIOLOGY OF REPRODUCTION, 2010
      • Identification of Oog1 Promoter Regions which Function During the First Meiosis in Oocytes.
        Eriko Okazaki; Satoshi Tsukamoto; Koji Kimura; Yuki Ohta; Seiji Kito; Hiroshi Imai; Naojiro Minami
        BIOLOGY OF REPRODUCTION, 2010, Peer-reviewed
      • ウシ精巣中の性成熟にともなう生殖幹細胞の動態と体外培養について
        藤原摩耶子; 金聖民; 南直治郎; 山田雅保; 今井裕
        J Reprod Dev, 25 Aug. 2009
      • ZP3 Promoter Is Not Enough to Analyze the Early Expressing Genes During Oogenesis, Such as Oog1
        Hisashi Imaichi; Rieko Okazaki; Satoshi Tsukamoto; Yuki Ohta; Seiji Kito; Koji Kimura; Hiroshi Imai; Naojiro Minami
        BIOLOGY OF REPRODUCTION, 2009
      • Analysis of culture condition for generation of induced pluripotent stem (iPS) cells in various mammalian species
        TSUKIYAMA Tomoyuki; ASANO Ryota; MINAMI Naojiro; YAMADA Masayasu; IMAI Hiroshi
        日本分子生物学会年会講演要旨集, 2009, Peer-reviewed
      • Stem cell potential and in vitro culture of bovine gonocytes from the neonatal testis
        FUJIHARA Mayako; KIM Sung Min; MINAMI Naojiro; YAMADA Masayasu; IMAI Hiroshi
        日本分子生物学会年会講演要旨集, 2009, Peer-reviewed
      • ZP3 promoter is not enough to analyze the early expressing genes during oogenesis, such as Oog1 Analysis of Oog1, an oocyte-specific gene, using transgenic RNAi approach.
        Imaichi H; Okazaki E; Tsukamoto S; Ohota Y; Kito S; Kimura K; Imai H; Minami N
        42nd Society for the Study of Reproduction, 2009, Peer-reviewed
      • NANOG is expressed by the progenitor-type stem cells and differentiated germ cells in pig testis
        S. Goel; M. Fujihara; N. Minami; M. Yamada; H. Imai
        REPRODUCTION IN DOMESTIC ANIMALS, Jul. 2008
      • Mammalian oocyte extracts induce chromatin remodeling and dedifferentiation of somatic cells, but do not global demethylation
        K. Miyamoto; T. Tsukiyama; N. Minami; M. Yamada; H. Imai
        REPRODUCTION IN DOMESTIC ANIMALS, Jul. 2008, Peer-reviewed
      • Analysis of Oog1, an oocyte-specific gene, using transgenic RNAi approach
        Hisashi Imaichi; Satoshi Tsukamoto; Yuki Ohta; Seiji Kito; Koji Kimura; Hiroshi Imai; Naojiro Minami
        BIOLOGY OF REPRODUCTION, 2008
      • Preservation of porcine gonocytesat 4 degrees c and in liquid nitrogen - A preservation model of genetic resources in domestic animals and in endangered species
        M. Fujihara; S. Goel; Y. Kimura; N. Minami; M. Yamada; H. Imai
        REPRODUCTION FERTILITY AND DEVELOPMENT, 2008, Peer-reviewed
      • DNA demethylation associated with nuclear reprogramming of the somatic cell genome in cell-free extracts from mammalian oocytes
        Kei Miyamoto; Tomoyuki Tsukiyama; Yang Yang; Ning Li; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        BIOLOGY OF REPRODUCTION, 2008, Peer-reviewed
      • Oog1 is a maternal effect gene required for the development of mouse preimplantation embryo
        N. Minami; H. Imaichi; S. Tsukamoto; Y. Ohta; S. Kito; K. Kimura; H. Imai
        REPRODUCTION IN DOMESTIC ANIMALS, Jul. 2008, Peer-reviewed
      • Reprogramming of mouse embryonic fibroblasts by cell-free extracts from nanog-overexpressing embryonic stem cells
        Tomoyuki Tsukiyama; Kei Miyamoto; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
        BIOLOGY OF REPRODUCTION, 2008, Peer-reviewed
      • Effective suppression of Oog1, an oocytespecific gene, by RNAi.
        Satoshi Tsukamoto; Hisashi Imaichi; Yuki Ohta; Seiji Kite; Akira Aizawa; Hiroshi Imai; Naojiro Minami
        BIOLOGY OF REPRODUCTION, 2007
      • Zygotically activated genes are suppressed in mouse nuclear transferred embryos
        Suzuki T; Minami N; Imai H
        33rd International Embryo Transfer Society, 2007, Peer-reviewed
      • Zygotically activated genes are suppressed in mouse nuclear-transferred embryos
        T. Suzuki; N. Minami; H. Imai
        REPRODUCTION FERTILITY AND DEVELOPMENT, 2007, Peer-reviewed
      • Preservation of gonocytes in pigs.
        Fujihara M; Goel S; Kimura Y; Minami N; Yamada M; Imai H
        The 4th Asian Reproductive Biotechnology Conference, 2007, Peer-reviewed
      • NANOG is expressed by progenitor-type stem cells and spermatocytes in porcine testis.
        Goel S; Fujihara M; Minami N; Yamada M; Imai H
        The 4th Asian Reproductive Biotechnology Conference, 2007, Peer-reviewed
      • Differential nuclear reprogramming of somatic cells by porcine germinal vesicle and metaphase II oocyte extracts.
        Miyamoto K; Tsukiyama T; Minami N; Yamada M; Imai H
        The 4th Asian Reproductive Biotechnology Conference, 2007, Peer-reviewed
      • Management system for frozen bull semen using a radio frequency identification tag
        S. Kato; K. Mukojima; T. Otani; K. Sakai; K. Tamura; K. Miyazaki; N. Minami; H. Imai
        REPRODUCTION FERTILITY AND DEVELOPMENT, 2007, Peer-reviewed
      • Lectin Dolichos Biflourus Agglutinin (DBA) is a specific marker for primitive germ cells in neonatal porcine testis
        S. Goel; M. Sugimoto; S. Kume; M. Yamada; N. Minami; H. Imai
        REPRODUCTION FERTILITY AND DEVELOPMENT, 2007, Peer-reviewed
      • Nuclear reprogramming of porcine cells and their use as donor cell for nuclear transfer after treatment in Xenopus egg extracts
        K. Miyamoto; M. Ohnuki; N. Minami; M. Yamada; H. Imai
        REPRODUCTION FERTILITY AND DEVELOPMENT, 2007, Peer-reviewed
      • Investigation of Oog1, an oocyte-specific gene, using RNAi approach
        Tsukamoto S; Imaichi H; Kito S; Ohota Y; Aizawa A; Imai H; Minami N
        40th Society for the Study of Reproduction, 2007, Peer-reviewed
      • Nuclear reprogramming of porcine fibroblast cells by Xenopus egg extracts.
        Miyamoto K; Nagao Y; Minami N; Yamada M; Ohsumi K; Imai H
        32nd International Embryo Transfer Society, 2006, Peer-reviewed
      • Comprehensive identification of relative genes causing abnormal development of bovine embryos produced by somatic cell nuclear transfer.
        Park J-S; Minami N; Imai H
        32nd International Embryo Transfer Society, 2006, Peer-reviewed
      • Suppressive expression of zygotically activated genes in mouse cloned embryos.
        Toru Suzuki; Naojiro Minami; Hiroshi Imai
        BIOLOGY OF REPRODUCTION, 2006, Peer-reviewed
      • Oog1, an oocyte-specific protein, interact with Ran during the first mitosis in mouse embryo.
        Satoshi Tsukamoto; Ryo Ihara; Akira Aizawa; Akira Nakanishi; Hiroshi Imai; Naojiro Minami
        BIOLOGY OF REPRODUCTION, 2006, Peer-reviewed
      • Reprogramming of intact and permeabilized mammalian somatic cells by Xenopus egg extract
        Miyamoto K; Ohnuki M; Minami N; Yamada M; Imai H
        The 3rd Asian Reproductive Biotechnology Conference, 2006, Peer-reviewed
      • Functional analysis of Oog1, an oocyte-specific gene, in mouse embryo
        Tsukamoto S; Imaichi H; Kito S; Ohota Y; Aizawa A; Imai H; Minami N
        The 3rd Asian Reproductive Biotechnology Conference, 2006, Peer-reviewed
      • Identification and characterization of two novel classes of small RNAs in the mouse germ line
        Watanabe T; Tsukiyama T; Sasaki H; Minami N; Imai H
        Mouse Molecular Genetics Cold Spring Harbor Laboratory, 2006, Peer-reviewed
      • Two novel classes of small RNAs from mouse germline
        Watanabe T; Takeda A; Tsukiyama T; Sasaki H; Minami N; Imai H
        International Genomic Imprinting Workshop, 2006, Peer-reviewed
      • The quantitative aberration of gene epression in the mouse NT embryos at the 2-cell stage
        SUZUKI Toru; MINAMI Naojiro; IMAI Hiroshi
        生化学, 2006, Peer-reviewed
      • Cell cycle synchronization of donor cells at G1 phase and developmental ability of nuclear transfer embryos in miniature pigs
        Miyamoto K; Hoshino Y; Nagao Y; Minami N; Yamada M; Imai H
        The Interational Embryo Transfer Society Meeting, Copenhagen, Denmark, 08 Jan. 2005, Peer-reviewed
      • Involvement of an oocyte-specific novel gene, Oog1, in zygotic genome activation via Ras signaling pathway.
        S Tsukamoto; R Ihara; A Aizawa; H Imai; N Minami
        BIOLOGY OF REPRODUCTION, 2005, Peer-reviewed
      • Analysis of gene expression of bovine embryos produced by somatic cell nuclear transfer.
        Park J-S; Minami N; Imai H
        15th International Congress of Animal Reproduction, 2004, Peer-reviewed
      • Single embryo analysis of the gene expression in the mouse 2-cell stage embryos reconstructed by somatic nuclear transfer.
        T Suzuki; N Minami; H Imai
        BIOLOGY OF REPRODUCTION, 2004, Peer-reviewed
      • Nuclear localization of Oogenesin, an oocyte- and embryo-specific novel gene in mouse preimplantation embryo
        N Minami; R Ihara; H Takayama; A Ohashi; A Aizawa; H Imai
        BIOLOGY OF REPRODUCTION, 2003
      • Phosphorylation of cyclin A2 is in accordance with the activation of CDC2 klnase.
        A Ohashi; N Minami; H Imai
        BIOLOGY OF REPRODUCTION, 2002
      • Analysis of an oocyte-specific novel gene, oogenesin, in the mouse.
        N Minami; M Miyamoto; A Ohashi; A Aizawa; D Kigami; H Imai
        BIOLOGY OF REPRODUCTION, 2002
      • 卵管環境下で培養されたマウス胚におけるE-カドヘリンの局在
        南 直治郎; 今井 裕
        Journal of mammalian ova research = 日本哺乳動物卵子学会誌, Apr. 2001
      • ウシ体細胞核移植および受精卵核移植における融合・活性化のタイミングとはい盤胞発生率の関係
        黒坂哲; 岩田尚孝; 松田達彦; 野村泰弘; 長尾恭光; 南直治郎; 山田雅保; 今井裕
        日本畜産学会大会講演要旨, 2001
      • Expression and functional analysis of an oocyte-specific novel gene, c-1, in the mouse.
        N Minami; M Miyamoto; A Aizawa; H Imai
        BIOLOGY OF REPRODUCTION, 2001
      • Cdc2 kinase activation regulates nuclear accumulation of cyclin B1 and modification of cyclin A in mouse 2-cell embryos.
        A Ohashi; N Minami; Y Nagao; M Yamada; H Imai
        BIOLOGY OF REPRODUCTION, 2001
      • Relationship between activation and fusion on developmental ability of bovine nuclear transfer embryos.
        Kurosaka S; Nagao Y; Minami N; Yamada M; Imai H
        Theriogenology 55, 240., Jan. 2001, Peer-reviewed
      • ミニブタ体細胞核移植胚の発生
        星野洋一郎; 長尾泰光; 南直治郎; 山田雅保; 今井 裕
        第7回日本胚移植研究会、講演要旨集、p. 30, 25 Aug. 2000
      • ウシ体細胞核移植におけるシクロヘキシミド処理時間の影響
        黒坂哲; 大橋紹宏; 高橋清也; 長尾恭光; 南直治郎; 山田雅保; 今井裕
        日本畜産学会大会講演要旨, 2000
      • Effect of recipient cytoplasm following oocyte activation on the development of nuclear transferred embryos from somatic cells in the bovine.
        S Kurosaka; A Ohashi; Y Nagao; N Minami; M Yamada; H Imai
        BIOLOGY OF REPRODUCTION, 2000
      • A novel gene of mouse early preimplantation embryos expressed in an environment dependent manner.
        N Minami; M Miyamoto; K Sasaki; A Aizawa; A Ohashi; H Imai
        BIOLOGY OF REPRODUCTION, 2000
      • Nuclear accumulation of cyclin B is inhibited in mouse embryos arrested at the 2-cell stage in vitro.
        A Ohashi; N Minami; M Miyamoto; Y Nagao; M Yamada; H Imai
        BIOLOGY OF REPRODUCTION, 2000
      • Intra-specific transfer of liver mitochondria and their fate during embryogenesis after microinjection into mouse zygotes.
        Muramatsu, H; Nagao, Y; Minami, N; Yamada, M; Imai, H
        2000 International Embryo Transfer Society Meeting, Maastricht, Netherland, Abstract、Theriogenology, 53 (1), 398., Jan. 2000, Peer-reviewed
      • 電気刺激による細胞融合法を用いたマウス体細胞の核移植
        浅井歩; 長尾恭光; 黒坂哲; 南直治郎; 山田雅保; 今井裕
        日本畜産学会大会講演要旨, 1999
      • Effects of cumulus cell density during in vitro maturation on the developmental competence of bovine oocytes (vol 48, pg 1451, 1998)
        S Hashimoto; K Saeki; Y Nagao; N Minami; M Yamada; K Utsumi
        THERIOGENOLOGY, Jul. 1998
      • Effects of aspiration vacuum and needle diameter on the recovery rate of bovine cumulus-oocyte-complexes by transvaginal aspiration
        S Hashimoto; R Takakura; M Kishi; T Sudo; N Minami; M Yamada
        THERIOGENOLOGY, Jan. 1998
      • A suitable passaging technique for the establishment of bovine embryonic stem (ES)-like cell lines
        R Parnpai; N Minami; M Yamada; K Utsumi
        THERIOGENOLOGY, Jan. 1998
      • Relationship between p34(cdc2) kinase activity and developmental competence of 2-cell mouse embryos cultured in an oviductal environment.
        N Minami; T Kojima; M Yamada; K Utsumi
        BIOLOGY OF REPRODUCTION, 1997
      • E-cadherin localization and gap junction assembly in the mouse embryos are accelerated by the oviduct in vitro.
        N Minami; A Takahashi; M Yamada; K Utsumi
        BIOLOGY OF REPRODUCTION, 1996

      Presentations

      • マウス初期胚におけるH4K20のメチル化状態の変化に関する研究
        山本琢人; 四方大樹; 池田俊太郎; 南直治郎
        第64回日本生殖医学会学術講演会, 09 Nov. 2019
      • マウス初期胚発生におけるヒストンアルギニンメチル化酵素Prmt6の役割
        本多慎之介; 国本悠里; 池田俊太郎; 南直治郎
        第112回日本繁殖生物学会, 03 Sep. 2019
      • ウシ初期胚のヒストンメチル化におけるメチオニンアデノシルトランスフェラーゼの役割
        太田愛里; 池田俊太郎; 杉本美紀; 南直治郎; 太田毅
        第112回日本繁殖生物学会, 03 Aug. 2019
      • SETD8/PRSET7-mediated histone H4K20 monomethylation is required for mouse preimplantation development
        Daiki Shikata; Takuto Yamamoto; Shinnosuke Honda; Naojiro Minami
        The 52nd Annual Meeting of the Society for the Study of Reproduction, 18 Jul. 2019
      • Analysis of oocyte-specific multi-copy gene,?Oog1?using CRISPR/Cas9 system
        Yuri Kunimoto; Yuka Miki; Satoshi Tsukamoto; Takuro Horii; Izuho Hatada; Naojiro Minami
        The 52nd Annual Meeting of the Society for the Study of Reproduction, 18 Jul. 2019
      • Role of Prmt6 and asymmetric dimethylation of H3R2 on mouse preimplantation embryos
        Honda Shinnosuke; Yuri Kunimoto; Naojiro Minami
        The 52nd Annual Meeting of the Society for the Study of Reproduction, 18 Jul. 2019
      • Functional analysis of Pwp1 during early embryogenesis in the mouse
        Atsushi Takasu; Daiki Shikata; Naojiro Minami
        The 52nd Annual Meeting of the Society for the Study of Reproduction, 18 Jul. 2019
      • Histone lysine methyltransferase SETD8 controls cell cycle in mouse preimplantation development
        Takuto Yamamoto; Daiki Shikata; Naojiro Minami
        The 52nd Annual Meeting of the Society for the Study of Reproduction, 18 Jul. 2019
      • マウス初期胚の発生におけるH4K20メチル化修飾の役割
        四方大樹; 山本琢人; 本多慎之介; 南直治郎
        第13回日本エピジェネティクス研究会年会, 28 May 2019
      • マウス初期胚発生におけるH4K20メチル化修飾の機能解析
        四方大樹; 本多慎之介; 山本琢人; 南直治郎
        第41回日本分子生物学会年会, 28 Nov. 2018
      • Knockout analysisi of oocyte-specific multi-copy gene, Oog1
        Miki Y; Tsukamoto S; Imai H; Minami N
        51st Annual Meeting of the Society for the Study of Reproduction, 10 Jul. 2018
      • CHD1はマウス初期胚において胚性ゲノムの活性化時のHmgpiの発現を介して細胞の運命決定を制御している
        南 直治郎
        日本分子生物学会, 07 Dec. 2017, 日本分子生物学会, Invited
      • CHD1 controls cell lineage specification through zygotic genome activation
        Minami, N
        The 6th International Symposium of Center for Animal Bioreactor & Xenotransptantation, 30 Nov. 2017, Invited
      • Functional analysis of histone demethylase Kdm5b in mouse preimplantation development.
        Kurasaka M; Kaneko T; Suzuki S; Tsukamoto S; Imai H; Minami N
        4th World Congress of Reproductive Biology, 27 Sep. 2017
      • The role of SMYD3 in methylation of H3K4 of Hmgpi promoter regions
        Honda S; Suzuki S; Tsukamoto S; Kaneko T; Imai H; Minami N
        4th World Congress of Reproductive Biology, 27 Sep. 2017
      • A long-term culture system supplemented with 6-bromoindirubin-3’-oxime (bio) maintains proliferation of germ cells from adult testis in cattle
        Suyatno; Kitamura Y; Minami N; Yamada M; Imai H
        50th Annual Meeting of the Society for the Study of Reproduction, 13 Jul. 2017
      • Methylation of H3K4 of Hmgpi promoter region by SMYD3 in mouse preimplantation embryos
        Honda S; Suzuki S; Tsukamoto S; Kaneko T; Imai H; Minami N
        50th Annual Meeting of the Society for the Study of Reproduction, 13 Jul. 2017
      • Improvement of long-term culture system for bovine spermatogonial stem cells
        Suyatno; Kitamura Y; Minami N; Yamada M; Imai H
        The 7th Congress of the Asian Pacific Invitative on Reproduction, 30 Mar. 2017
      • Induction of differentiation of bovine male germ cells in culture
        Kitamura Y; Suyatno; Minami N; Yamada M; Imai H
        The 7th Congress of the Asian Pacific Invitative on Reproduction, 30 Mar. 2017
      • CHD1 is a zygotic determinant for cell lineage specification via the HMGPI pathway in mouse preimplantation embryo.
        MINAMI Naojirou
        49th Annual Meeting of the Society for the Study of Reproduction, 18 Jul. 2016, Society for the Study of Reproduction, Invited
      • Epigentic states of chicken fibroblasts are changed during reprogramming processes by pH in culture
        Kim N; Minami N; Yamada M; Imai H
        18th International Congress on Animal Reproduction, 26 Jun. 2016
      • BMP4 is involved in maintenance or derivation of bovine primordial germ cells from bovine induced pluripotent stem cells
        Hayashi M; Cho D; Kawaguchi T; Minami N; Yamada M; Imai H
        18th International Congress on Animal Reproduction, 26 Jun. 2016
      • HSP90 regulates the pluripotency-related pathways and supports normal preimplantation embryonic development in mice
        Cho D; Phuong LQ; Minami N; Yamada M; Imai H
        18th International Congress on Animal Reproduction, 26 Jun. 2016
      • In vivo analysis of processing bodies using transgenic mice expressing GFP-Dcp1a.
        Tsukamoto S; Hara T; Sato K; Minami N; Kito S
        15th International Congress of Radiation Research, 25 May 2015
      • The role of Oog1 in female meiotic germ cells -activation of oocyte-specific genes and suppression of testis-associated genes-
        Miyamoto Y; Kawahara Y; Suzuki S; Ishida M; Tsukamoto S; Imai H; Minami N
        IFFS/JSRM International Meeting 2015, 26 Apr. 2015
      • CHD1 Controls Cell Lineage Specification Through Zygotic Genome Activation.
        Suzuki S; Minami N
        Springer, 2018, Not refereed
      • 卵子学
        南 直治郎
        京都大学学術出版会, 2011, Not refereed
      • Identification and Expression Analysis of Small RNAs During Development
        Watanabe, T; Imai, H; Minami N
        Methods Mol Bio. 2008; 442: 173-185., 2008, Not refereed
      • Analysis of small RNA profiles during development.
        Watanabe T; Totoki Y; Sasaki H; Minami N; Imai H
        Methods Enzymol., 2007, Not refereed
      • ウシ胚盤胞期胚のcDNAライブラリー構築法
        塚本智史; 今市寿史; 木村康二; 今井 裕; 南 直治郎
        日本胚移植研究学雑誌, 2006, Not refereed
      • ウシ体細胞クローン胚における遺伝子発現パターンの解析
        南 直治郎; 朴 志秀
        畜産技術協会, 2004, Not refereed
      • Gene expression and developmental competence of mouse 2-cell embryos cultured in vitro. In "Reproductive Biotechnology and Relating Physiology", (Miyamoto, H. and Manabe, N. eds.)
        N. Minami
        Hokuto Shobo,155-160, 2001, Not refereed
      • Cytoplasmic environment and cell cycle co-ordination of recipient and donor cells in nuclear transfer embryos. In "Reproductive Biotechnology and Relating Physiolog
        S. Kurosaka; N. Minami; H. Imai
        Hokkuto Shobo,267-271, 2001, Not refereed
      • In vitro maturation of bovine oocytes under low oxygen tension. In "Reproductive Biotechnology and Relating Physiology"
        S. Hashimoto; N. Minami
        Hokuto Shobo,229-233, 2001, Not refereed
      • Nuclear accumulation of cyclin B1 in mouse 2-cell embryos: Involvement of the activation of Cdc2. In "Reproductive Biotechnology and Relating Physiolog
        A. Ohashi; N. Minami
        Hokuto Shobo,297-301, 2001, Not refereed
      • Current status of research for the establishment of bovine embryonic stem (ES) cells. In "Reproductive Biology Update-Novel Tools for Assessment of Environmental Toxicity-", (Miyamoto, H. and Manabe, N ed. ).
        R. Parnpai; N. Minami; M. Yamada
        Shoukadoh Booksellers Company,/, 383-388, 1998, Not refereed
      • Expression and activation of nuclear factor kappa B during mouse preimplantation development. In "Reproductive Biology Update-Novel Tools for Assessment of Environmental Toxicity-", (Miyamoto, H. and Manabe, N ed. ).
        A. Nishikimi; J. Mukai; N. Minami; M. Yamada
        Shoukadoh Booksellers Company,/, 183-187, 1998, Not refereed
      • Extraembryonic environment and development of preimplantation rat embryos. In "Reproductive Biology Update-Novel Tools for Assessment of Environmental Toxicity-", (Miyamoto, H. and Manabe, N id. ).
        M. Yamada; A. Nishikimi; N. Uekawa; K. Hoshino; N. Minami
        Shoukadoh Booksellers Company,/, 355-364, 1998, Not refereed
      • Second cell cycle and cell adhesion of mouse embryos developed in an oviductal environment in vitro. In "Reproductive Biology Update-Novel Tools for Assessment of Environmental Toxicity-", (Miyamoto, H. and Manabe, N ed. ).
        N. Minami; A. Ohashi; K. Sasaki; T. Wada; M. Yamada
        Shoukadoh Booksellers Company,/, 345-353, 1998, Not refereed
      • Early embryonic development under oviductal influence in vitro.
        N. Minami
        Anim. Reprod. Sci., 1996, Not refereed
      • Embryo culture in explanted oviducts in mice and cattle.Endocrine regulation of early embryonic development and implantation.
        Hosoi Y; Minami N; Iritani A
        Hormone Research, 1995, Not refereed
      • ホ乳動物初期胚の発生におよぼす卵管の影響
        南 直治郎
        受精着床学会誌, 1994, Not refereed

      Industrial Property Rights

      • 特願特許出願2006-264064, 無線ICタグ封入ストロー

      Works

      • ウシ体細胞クローン胚の遺伝子発現解析
        From 2002, To 2003
      • Analysis of gene expression of bovine nuclear transfer embryos
        From 2002, To 2003
      • 精子分別による雌雄産み分けの実用化
        From 2001
      • Practical application of sex selection of calf by sperma separation
        From 2001

      Awards

      • 2004
        Royan Institute, Novel Maternal Effect Gene, Oogenesin: Involvement in Zygotic Gene Activation and Early Embryonic Development in The Mouse

      External funds: Kakenhi

      • 金ナノ粒子とレーザー光を利用した受精卵の分化制御機構の解明
        Grant-in-Aid for Challenging Research (Exploratory)
        Medium-sized Section 42:Veterinary medical science, animal science, and related fields
        Kyoto University
        南 直治郎
        From 28 Jun. 2019, To 31 Mar. 2022, Granted
        初期胚;遺伝子発現;金ナノ粒子;分化制御;HSPプロモーター;受精卵;遺伝子導入;割球の運命決定
      • Epigenetics from totipotency acquisition to cell differentiation during early embryogenesis
        Grant-in-Aid for Scientific Research (B)
        Basic Section 42030:Animal life science-related
        Kyoto University
        南 直治郎
        From 01 Apr. 2019, To 31 Mar. 2023, Granted
        初期胚;エピジェネティクス;ヒストン修飾;全能性;細胞分化;Prmt6;H3R2;ERV;分化;H4K20me1;マウス初期胚;SETD8;H4K20M置換体
      • Epigenetics from zygotic genome activation to implantation controlling the totipotency of the preimplantationembryo
        Grant-in-Aid for Scientific Research (B)
        Kyoto University
        Naojiro Minami
        From 01 Apr. 2016, To 31 Mar. 2019, Project Closed
        マウス初期胚;胚性ゲノムの活性化;エピジェネティクス;発生と分化;全能性;クロマチンリモデリング;ヒストン;受精卵;ノックダウン;多能性;初期発生とエピジェネティクス;マウス初期発生;細胞の運命決定;ヒストン修飾酵素;初期胚;クロマチン;遺伝子発現
      • Study on birth separation of mammals using CRISPR / Cas 9 system
        Grant-in-Aid for Challenging Exploratory Research
        Kyoto University
        Naojiro Minami
        From 01 Apr. 2014, To 31 Mar. 2017, Project Closed
        産み分け;CRISPR/Cas9;ゲノム編集;ノックイン;Crispr/Cas9;性染色体;受精阻害
      • Study on the production of sex-selected offspring in mammals using fertilization inhibitory effect
        Grant-in-Aid for Challenging Exploratory Research
        Kyoto University
        NAOJIRO MINAMI
        From 01 Apr. 2012, To 31 Mar. 2014, Project Closed
        産み分け;遺伝子組換え;マウス;受精阻害;細胞間架橋;性染色体
      • Epigenetic analysis of zygotic gene activation by maternal factor
        Grant-in-Aid for Scientific Research (B)
        Kyoto University
        Naojiro MINAMI
        From 01 Apr. 2011, To 31 Mar. 2015, Project Closed
        マウス初期胚;エピジェネティクス;胚性ゲノムの活性化;遺伝子発現;母性因子;siRNA;多能性;分化;Smyd3;Epigenetics;mouse embryo;development;Oct4;Cdx2;受精卵;ヒストンメチル化;Chd1;タンパク質分解;ユビキチンプロテアソーム;リソソーム;マウス;初期胚;クロマチンリモデリング
      • Functional analysis of autophagy during spermatogenesis in mice
        Grant-in-Aid for Young Scientists (A)
        National Institute of Radiological Sciences
        Satoshi TSUKAMOTO
        From 01 Apr. 2010, To 31 Mar. 2013, Project Closed
        オートファジー;精巣;精子形成;ノックアウトマウス;不妊
      • Involvement of autophagy on sperm mitochondria degradation in mice
        Grant-in-Aid for Challenging Exploratory Research
        National Institute of Radiological Sciences
        Satoshi TSUKAMOTO
        From 01 Apr. 2010, To 31 Mar. 2012, Project Closed
        オートファジー;ミトコンドリア;精子;受精卵;マウス
      • 精子形成期の半数体特異的遺伝子発現を利用した単性動物の作出
        Grant-in-Aid for Challenging Exploratory Research
        Kyoto University
        南 直治郎
        From 01 Apr. 2009, To 31 Mar. 2011, Project Closed
        半数体特異的;精子形成;細胞間架橋;遺伝子組換え;Smokl;非翻訳領域;受精
      • ほ乳類精子形成期の半数体特異的発現遺伝子を利用した単性動物の創造
        Grant-in-Aid for Exploratory Research
        Kyoto University
        南 直治郎
        From 01 Apr. 2007, To 31 Mar. 2009, Project Closed
        雌雄産み分け;半数体;精子形成;遺伝子発現;トランスジェニック
      • Analysis of oocyte-specific gene, Oog1 and its involvement in molecular basis of zygotic gene activation
        Grant-in-Aid for Scientific Research (B)
        Kyoto University
        Naojiro MINAMI
        From 01 Apr. 2007, To 31 Mar. 2010, Project Closed
        発生工学;Oog1;胚性ゲノム;プロモーター活性;卵母細胞;胚性ゲノムの活性化;RNAi;遺伝子発現;初期発生;0og1
      • Functional analysis of a novel gene, Oogenesin, which localizes to the nucleus at the time of ZGA
        Grant-in-Aid for Scientific Research (B)
        KYOTO UNIVERSITY
        Naojiro MINAMI
        From 01 Apr. 2004, To 31 Mar. 2007, Project Closed
        Oog1;卵子特異的;胚性ゲノムの活性化;核移行;RalGDS;Ran;Importinβ;Chchd6;Yeast Two-Hybrid;Rasシグナル, Oog1;oocyte-specific;zygotic gene activation;nuclear localization;RalGDS;Ran;Importin β
      • Functional analysis of oocyte-specific gene, c-1, which express during meiosis
        Grant-in-Aid for Scientific Research (C)
        KYOTO UNIVERSITY
        Naojiro MINAMI
        From 01 Apr. 2002, To 31 Mar. 2004, Project Closed
        Oogenesin;卵子特的遺伝子;ロイシンジッパー;核移行;胚性ゲノムの活性化;母性効果遺伝子;マウス初期胚;卵母細胞;卵子特異的;遺伝子発現;胚ゲノム活性化;転写因子, Oogenesin;oocyte-specific gene;leucine Zipper;nuclear localization;zygotic gene expression;maternal effect gene
      • Effect of different type of mitochondrial DNA on germline-differentiating potency of ES cell line in the mouse
        Grant-in-Aid for Scientific Research (C)
        KYOTO UNIVERSITY
        Yasumitsu NAGAO
        From 01 Apr. 2000, To 31 Mar. 2002, Project Closed
        ES細胞;ミトコンドリアDNA;精子;生殖系列キメラ;全能性;精子受精能;キメラ形成能;同胞異種;胚性幹細胞, ES cell;mitochondrial DNA;sperm;germ-line chimera;totipotency
      • Studies on cell cycle and gene expression of mammalian preimplantation embryos cultured in an oviductal environment
        Grant-in-Aid for Scientific Research (C)
        KYOTO UNIVERSITY
        Naojiro MINAMI
        From 01 Apr. 1998, To 31 Mar. 2000, Project Closed
        マウス胚;遺伝子発現;卵管環境;differential display;細胞周期;サイクリンB;mouse embryo;2-cell stage;gene expression;oviductal environment, mouse embryo;gene expression;cell cycle;oviductal environment;differential display;cyclin B
      • 精子ベクター法の開発-核移行およびDNA結合シグナルペプチドの応用-
        Grant-in-Aid for Exploratory Research
        Kyoto University
        山田 雅保
        From 01 Apr. 1996, To 31 Mar. 1998, Project Closed
        遺伝子導入;単節型核移行シグナル;双節型核移行シグナル;細胞質インジェクション;核移行シグナルペプチド;ポリLリジン
      • Development of bovine eggs collected from ovaries of live animals with ultrasound-guided system
        Grant-in-Aid for Scientific Research (A)
        KYOTO UNIVERSITY
        Masayasu YAMADA
        From 01 Apr. 1996, To 31 Mar. 1999, Project Closed
        ウシ卵母細胞;経膣採卵;超音波画像誘導システム;過剰排卵;卵胞吸引;抗インヒビン抗体;体外成熟;ミッドカイン;超音波画像;卵胞液吸引;インヒビン;細胞質成熟促進活性;バキュロウイルス;細胞質成熟促進因子;遺伝子発現;体外培養;RT-PCR法;CCDイメージカメラ, Bovine oocyte;transvaginal collection;ultrasound guided-system;superovulation;follicular aspiration;anti-inhibin antibody;in vitro maturation;midkine
      • Molecular regulation of gene expression and development of mammalian embryos
        Grant-in-Aid for Scientific Research (A)
        KYOTO UNIVERSITY
        Masayasu YAMADA
        From 01 Apr. 1996, To 31 Mar. 1999, Project Closed
        着床前発生;胚性ゲノムの活性化;レドックス制御;NF-KB;一酸化窒素;胚盤胞形成;核タンパク;mRNAスプライシング因子;着床前胚の発生;転写因子NF-KB;100kDa核内タンパク;細胞周期;一酸化窒素ラジカル(NO);胞胚腔形成;NFKB;一酸化窒素ラジカル;初期胚の遺伝子発現;NFkB;CCDイメージアナライザー, preimplantation development;zygotic gene activation;redox regulation;NF-kB;nitric oxide;blastocyst formation;nuclear protein;mRNA splicing factor
      • Studies on the cell adhesion and cell to cell communication during development and differentiation in mammalian preimplantation embryos
        Grant-in-Aid for Scientific Research (C)
        KYOTO UNIVERSITY
        Naojiro MINAMI
        From 01 Apr. 1995, To 31 Mar. 1997, Project Closed
        マウス胚;発生;卵管;E-カドヘリン;Gap Junction;プロテインキナーゼC;p34^キナーゼ;マウス初期発生;ギャプ結合;p34cdc2キナーゼ;mouse embryo;oviduct;E-cadherin;gap junction;protein kinase C, Mouse embryo;Development;Oviduct;E-cadherin;Gap Junction;Protein kinase C;p34^ kinase
      • 哺乳動物初期胚の発生に関与する卵管因子と細胞間接着に関する研究
        Grant-in-Aid for Encouragement of Young Scientists (A)
        Kyoto University
        南 直治郎
        From 01 Apr. 1994, To 31 Mar. 1995, Project Closed
        マウス胚;E-カドヘリン;gap junction;connexin43;細胞間接着;卵管因子;in situハイブリダイゼーション
      • 卵管由来因子とマウス初期胚の発生過程に形成されるGap Junctionについて
        Grant-in-Aid for Encouragement of Young Scientists (A)
        Kyoto University
        南 直治郎
        From 01 Apr. 1993, To 31 Mar. 1994, Project Closed
        マウス;初期胚;卵管因子;Gap Junction;発生;Connexin43;in situハイブリダイゼーション
      • Role of cell surface carbohydrates on the development of mammalian embryos in vitro
        Grant-in-Aid for General Scientific Research (C)
        KYOTO UNIVERSITY
        Masayasu YAMADA
        From 01 Apr. 1993, To 31 Mar. 1996, Project Closed
        着床前期;初期胚;体外培養;PHA-Pレクチン;コンパクション;N結合型糖鎖;卵管抽出物;ラット;発生;PHAレクチン;卵管因子;マウス;糖鎖;Nグリカナーゼ, preimplantation;embryo;in vitro development;PHA-P lectin;compaction;N-linled carbohydrates;oviduct extracts
      • Development of embryo transfer system of bovine embryos sexed with PCR
        Grant-in-Aid for Developmental Scientific Research (B)
        KYOTO UNIVERSITY
        Masayasu YAMADA
        From 01 Apr. 1993, To 31 Mar. 1996, Project Closed
        胚移植;性判別;ウシSRYcDNA;PCR法;凍結保存;IVM;IVF由来ウシ胚;ウシSRY遺伝子;胚の性判別;ウシSRYの発現;性判別胚の凍結保存;性判別胚の移植;遺伝子増幅法;性分別;移植システム;ウシ精巣決定遺伝子(bSRY);ウシ胚;経膣採卵;無血清培養, Embryo transfer;Embryo sexing;Bovine SRY cDNA;PCR method;Cryopreservation;IVM;IVF bovine embryos
      • Analysis of mechanisms of male differentiation with sex determining genes in mammals
        Grant-in-Aid for General Scientific Research (B)
        KYOTO UNIVERSITY
        Hiroshi SAKAI
        From 01 Apr. 1992, To 31 Mar. 1995, Project Closed
        雄性特異的DNA;ウシ精巣決定遺伝子(bSRY);雄性分化機構;bSRY融合タンパク;性決定遺伝子;SRY;胚の性判別;ウシ;PCR法;デオキシ鎖反応;性分化;BOV97M, Male specific DNA;Bovine SRY gene;Male sex differentiation;bSRY fusion proteins

      External funds: others

      • 食資源動物の発生工学の新展開による効率増産
        卵管環境制御による初期胚の遺伝子発現支配
        日本学術振興会, 未来開拓事業
        入谷 明
        分担
      • 精子分別による雌雄産み分けの実用化
        家畜改良事業団, 畜産新技術開発活用促進事業
        From 2001, To 2001
        南 直治郎
        代表
      • ウシ体細胞クローン胚の遺伝子発現解析
        伊藤記念財団, 研究助成金
        From 2001, To 2002
        南 直治郎
        代表
      • ウシ体細胞クローン胚の遺伝子発現解析
        畜産技術協会, 委託研究開発
        From 2002, To 2003
        南 直治郎
        代表
      • 卵母細胞に特異的に発現する遺伝子の機能解析と生殖工学への応用
        稲盛財団, 稲盛財団学術研究助成金
        From 2004, To 2004
        南 直治郎
        代表
      • 和牛精液等流通管理体制構築推進事業
        農林水産省, 農業競争力強化対策事業推進費補助金
        From 2007, To 2011
        今井 裕
        分担
      • 哺乳動物における産み分け技術の開発
        科学技術振興機構, シーズ発掘試験
        From 2009, To 2009
        南 直治郎
        代表
      • ほ乳動物における革新的産み分け技術の開発
        探索タイプ
        科学技術振興機構, 研究成果最適展開支援事業フィージビリティスタディ
        From 2010, To 2010
        南 直治郎
        代表
      • 受精阻害機構を利用したほ乳動物の産み分け技術の開発
        探索タイプ
        科学技術振興機構, 研究成果最適展開支援プログラム
        From 2011, To 2012
        南 直治郎
        代表
      • 牛受精卵生産工程の品質管理技術開発事業 (2021年度分)
        日本中央競馬会, 日本中央競馬会畜産振興交付金
        From 01 Apr. 2021, To 31 Mar. 2022
        代表
      • 牛受精卵生産工程の品質管理技術開発事業
        JRA畜産振興事業
        From 01 Apr. 2021, To 31 Mar. 2024
        代表
      list
        Last Updated :2022/10/02

        Education

        Teaching subject(s)

        • From 01 Apr. 2022, To 31 Mar. 2023
          Animal Reproduction
          5156, Spring, Faculty of Agriculture, 2
        • From 01 Apr. 2022, To 31 Mar. 2023
          Outline of Bioresource Science A
          5112, Spring, Faculty of Agriculture, 2
        • From 01 Apr. 2022, To 31 Mar. 2023
          Seminar for Reproductive Biology1
          DB30, Year-long, Graduate School of Agriculture, 3
        • From 01 Apr. 2022, To 31 Mar. 2023
          Seminar for Reproductive Biology2
          DB31, Year-long, Graduate School of Agriculture, 3
        • From 01 Apr. 2022, To 31 Mar. 2023
          Laboratory Course for Reproductive Biology1
          DC30, Year-long, Graduate School of Agriculture, 6
        • From 01 Apr. 2022, To 31 Mar. 2023
          Laboratory Course for Reproductive Biology2
          DC31, Year-long, Graduate School of Agriculture, 6
        • 細胞生物学II
        • 昆虫・魚・哺乳動物の生理学
        • 生殖生物学入門 –ほ乳類の受精と初期発生-
        • From Apr. 2011, To Mar. 2012
          動物生理学
          Spring, 農学部
        • From Apr. 2011, To Mar. 2012
          細胞生物学II
          Spring, 農学部
        • From Apr. 2011, To Mar. 2012
          分子発生学特論
          農学研究科
        • From Apr. 2011, To Mar. 2012
          生殖生物学専攻実験
          農学研究科
        • From Apr. 2011, To Mar. 2012
          生殖生物学演習
          農学研究科
        • From Apr. 2012, To Mar. 2013
          Physiology
          Spring, 農学部
        • From Apr. 2012, To Mar. 2013
          Comparative Physiology
          Spring, 全学共通科目
        • From Apr. 2012, To Mar. 2013
          Cell Biology II
          Spring, 農学部
        • From Apr. 2013, To Mar. 2014
          Cell Biology II
          Spring, 農学部
        • From Apr. 2013, To Mar. 2014
          Molecular Embryology in Mammals
          Spring, 農学研究科
        • From Apr. 2013, To Mar. 2014
          Seminar for Reproductive Biology
          Year-long, 農学研究科
        • From Apr. 2013, To Mar. 2014
          Laboratory Course for Reproductive Biology
          Year-long, 農学研究科
        • From Apr. 2013, To Mar. 2014
          Comparative Physiology
          Spring, 全学共通科目
        • From Apr. 2014, To Mar. 2015
          Cell Biology II
          Spring, 農学部
        • From Apr. 2014, To Mar. 2015
          Seminar for Reproductive Biology
          Year-long, 農学研究科
        • From Apr. 2014, To Mar. 2015
          Laboratory Course for Reproductive Biology
          Year-long, 農学研究科
        • From Apr. 2014, To Mar. 2015
          Comparative Physiology
          Spring, 全学共通科目
        • From Apr. 2015, To Mar. 2016
          Molecular Embryology in Mammals
          Spring, 農学研究科
        • From Apr. 2015, To Mar. 2016
          Physiology
          Spring, 農学部
        • From Apr. 2015, To Mar. 2016
          Laboratory Course for Reproductive Biology1
          Year-long, 農学研究科
        • From Apr. 2015, To Mar. 2016
          Laboratory Course for Reproductive Biology
          Year-long, 農学研究科
        • From Apr. 2015, To Mar. 2016
          Seminar for Reproductive Biology1
          Year-long, 農学研究科
        • From Apr. 2015, To Mar. 2016
          Seminar for Reproductive Biology
          Year-long, 農学研究科
        • From Apr. 2015, To Mar. 2016
          Cell Biology II
          Spring, 農学部
        • From Apr. 2016, To Mar. 2017
          Physiology
          Spring, 農学部
        • From Apr. 2016, To Mar. 2017
          Laboratory Course for Reproductive Biology1
          Year-long, 農学研究科
        • From Apr. 2016, To Mar. 2017
          Laboratory Course for Reproductive Biology2
          Year-long, 農学研究科
        • From Apr. 2016, To Mar. 2017
          Laboratory Course for Reproductive Biology
          Year-long, 農学研究科
        • From Apr. 2016, To Mar. 2017
          Seminar for Reproductive Biology1
          Year-long, 農学研究科
        • From Apr. 2016, To Mar. 2017
          Seminar for Reproductive Biology2
          Year-long, 農学研究科
        • From Apr. 2016, To Mar. 2017
          Seminar for Reproductive Biology
          Year-long, 農学研究科
        • From Apr. 2016, To Mar. 2017
          Cell Biology II
          Spring, 農学部
        • From Apr. 2017, To Mar. 2018
          Molecular Embryology in Mammals
          Spring, 農学研究科
        • From Apr. 2017, To Mar. 2018
          Physiology
          Spring, 農学部
        • From Apr. 2017, To Mar. 2018
          Laboratory Course for Reproductive Biology1
          Year-long, 農学研究科
        • From Apr. 2017, To Mar. 2018
          Laboratory Course for Reproductive Biology2
          Year-long, 農学研究科
        • From Apr. 2017, To Mar. 2018
          Laboratory Course for Reproductive Biology
          Year-long, 農学研究科
        • From Apr. 2017, To Mar. 2018
          Seminar for Reproductive Biology1
          Year-long, 農学研究科
        • From Apr. 2017, To Mar. 2018
          Seminar for Reproductive Biology2
          Year-long, 農学研究科
        • From Apr. 2017, To Mar. 2018
          Seminar for Reproductive Biology
          Year-long, 農学研究科
        • From Apr. 2017, To Mar. 2018
          Cell Biology II
          Spring, 農学部
        • From Apr. 2017, To Mar. 2018
          Outline of Bioresource Science A
          Spring, 農学部
        • From Apr. 2018, To Mar. 2019
          Physiology
          Spring, 農学部
        • From Apr. 2018, To Mar. 2019
          Laboratory Course for Reproductive Biology1
          Year-long, 農学研究科
        • From Apr. 2018, To Mar. 2019
          Laboratory Course for Reproductive Biology2
          Year-long, 農学研究科
        • From Apr. 2018, To Mar. 2019
          Seminar for Reproductive Biology1
          Year-long, 農学研究科
        • From Apr. 2018, To Mar. 2019
          Seminar for Reproductive Biology2
          Year-long, 農学研究科
        • From Apr. 2018, To Mar. 2019
          Cell Biology II
          Spring, 農学部
        • From Apr. 2018, To Mar. 2019
          Outline of Bioresource Science A
          Spring, 農学部
        • From Apr. 2019, To Mar. 2020
          Molecular Embryology in Mammals
          Spring, 農学研究科
        • From Apr. 2019, To Mar. 2020
          Reproduction in Mammal
          Spring, 農学部
        • From Apr. 2019, To Mar. 2020
          Physiology
          Spring, 農学部
        • From Apr. 2019, To Mar. 2020
          Laboratory Course for Reproductive Biology1
          Year-long, 農学研究科
        • From Apr. 2019, To Mar. 2020
          Laboratory Course for Reproductive Biology2
          Year-long, 農学研究科
        • From Apr. 2019, To Mar. 2020
          Seminar for Reproductive Biology1
          Year-long, 農学研究科
        • From Apr. 2019, To Mar. 2020
          Seminar for Reproductive Biology2
          Year-long, 農学研究科
        • From Apr. 2019, To Mar. 2020
          Livestock Production Techniques and Practice II
          Spring, 農学部
        • From Apr. 2019, To Mar. 2020
          Cell Biology II
          Spring, 農学部
        • From Apr. 2019, To Mar. 2020
          Outline of Bioresource Science A
          Spring, 農学部
        • From Apr. 2020, To Mar. 2021
          Reproduction in Mammal
          Spring, 農学部
        • From Apr. 2020, To Mar. 2021
          Physiology
          Spring, 農学部
        • From Apr. 2020, To Mar. 2021
          Laboratory Course for Reproductive Biology1
          Year-long, 農学研究科
        • From Apr. 2020, To Mar. 2021
          Laboratory Course for Reproductive Biology2
          Year-long, 農学研究科
        • From Apr. 2020, To Mar. 2021
          Seminar for Reproductive Biology1
          Year-long, 農学研究科
        • From Apr. 2020, To Mar. 2021
          Seminar for Reproductive Biology2
          Year-long, 農学研究科
        • From Apr. 2020, To Mar. 2021
          Outline of Bioresource Science A
          Spring, 農学部
        • From Apr. 2021, To Mar. 2022
          Molecular Embryology in Mammals
          Spring, 農学研究科
        • From Apr. 2021, To Mar. 2022
          Reproduction in Mammal
          Spring, 農学部
        • From Apr. 2021, To Mar. 2022
          Physiology
          Spring, 農学部
        • From Apr. 2021, To Mar. 2022
          Practical Course in Applied Animal Science II
          Spring, 農学部
        • From Apr. 2021, To Mar. 2022
          Laboratory Course for Reproductive Biology1
          Year-long, 農学研究科
        • From Apr. 2021, To Mar. 2022
          Laboratory Course for Reproductive Biology2
          Year-long, 農学研究科
        • From Apr. 2021, To Mar. 2022
          Seminar for Reproductive Biology1
          Year-long, 農学研究科
        • From Apr. 2021, To Mar. 2022
          Seminar for Reproductive Biology2
          Year-long, 農学研究科
        • From Apr. 2021, To Mar. 2022
          Outline of Bioresource Science A
          Spring, 農学部

        Student achievements: Awards

        • 優秀発表賞
          本多 慎之介, 日本繁殖生物学会, 23 Sep. 2021
        • Outstanding Paper Award in 2020
          四方 大樹, 日本繁殖生物学会, 24 Feb. 2021
        • Outstanding Paper Award in 2018
          本多 慎之介, 日本繁殖生物学会, 12 Mar. 2019
        • Best International Abstract Award
          本多 慎之介, Society for the Study of Reproduction, 18 Jul. 2019
        • 優秀発表賞
          三木 祐果, Asian Reproductive Biotechnology Society, 03 May 2018
        • 優秀ポスター賞
          宮本 裕也, Asian Reproductive Biotechnology Society, 02 Nov. 2014
        • 優秀発表賞(ポスター部門)
          鈴木伸之介, 日本繁殖生物学会, 22 Aug. 2014
        • 優秀ポスター賞
          石田 未弥, Asian Reproductive Biotechnology Society, 10 Nov. 2010
        • 優秀発表賞
          塚本 智史, 日本繁殖生物学会, 21 Oct. 2007
        • Young Investigator Travel Award
          塚本 智史, Society for the Study of Reproduction, 24 Jul. 2005
        list
          Last Updated :2022/10/02

          Administration

          School management (title, position)

          • 組換えDNA実験安全委員会 委員
          • 京都大学動物実験委員会 委員
          • From 01 Apr. 2008, To 31 Mar. 2011
            組換えDNA実験安全委員会 委員
          • From 20 Nov. 2012, To 10 Oct. 2013
            京都大学動物実験委員会 外部評価導入WG 委員
          • From 01 Oct. 2018, To 30 Sep. 2020
            京都大学動物実験委員会 委員
          • From 01 Mar. 2020, To 31 Mar. 2023
            牧場 牧場長
          • From 01 Oct. 2020, To 30 Sep. 2022
            京都大学動物実験委員会 委員

          Faculty management (title, position)

          • From 01 Apr. 2013, To 31 Mar. 2015
            国際交流委員会 委員
          • From 01 Apr. 2006, To 31 Mar. 2023
            動物実験委員会 委員
          • From 01 Apr. 2016, To 31 Mar. 2018
            環境・安全・衛生委員会 委員
          • From 01 Apr. 2018, To 31 Mar. 2020
            制度・組織等検討委員会 委員
          • From 01 Apr. 2019, To 30 Sep. 2020
            動物実験委員会 委員長
          • From 01 Apr. 2020, To 31 Mar. 2024
            将来構想検討委員会
          • From 01 Apr. 2020, To 31 Mar. 2023
            評価委員会 委員
          • From 01 Mar. 2020, To 31 Mar. 2023
            農場・牧場連絡協議委員会 委員
          • From 01 Mar. 2020, To 31 Mar. 2023
            男女共同参画推進委員会 委員
          • From 01 Mar. 2020, To 31 Mar. 2023
            防火・防災委員会 委員
          list
            Last Updated :2022/10/02

            Academic, Social Contribution

            Committee Memberships

            • From Jun. 2018, To Present
              理事, 日本生殖医学会
            • From Apr. 2015, To Present
              理事, 日本繁殖生物学会
            • From Nov. 2011, To Present
              副編集委員長, 日本繁殖生物学会
            • From Jan. 1997, To Aug. 2019
              会計幹事, 日本胚移植研究会
            • From Apr. 2006, To Oct. 2009
              編集委員, 日本繁殖生物学会

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