Basic Information

Affiliated programs (koza)

• Graduate School of Medicine, 医学専攻 細胞機能調節学, 講師
• Graduate School of Medicine, 医科学専攻 細胞機能調節学, 講師
• Graduate School of Science, 生物科学専攻 生物物理学（分子細胞生物学）, 講師

Professional Memberships

• 日本生化学会
• 日本分子生物学会
• 日本細胞生物学会

Academic Degree

• 理学修士(甲南大学)
• 医学博士(京都大学)

Academic Resume (Graduate Schools)

• 甲南大学, 大学院自然科学研究科修士課程, 修了
• 京都大学, 大学院医学研究科博士課程, 修了

Academic Resume (Undergraduate School/Majors)

• Konan Univrcity, 理学部生物学科, 卒業

High School

• High School

  大阪星光学院

Language of Instruction

• English

ID,URL

• J-Global ID

  200901003418482882

researchmap URL

• https://researchmap.jp/read0012604

Research

Research Topics, Overview of the research

• Research Topics

  Analysis of general transcription mechanism
  Appplication of RNA aptamer

• Overview of the research

  We are focusing on two major items. 1)the investigation of eukaryotic gene expression mechanisms and 2) the functional analysis of collagen molecules using RNA aptamers, artificial RNA molecules selected from random RNA pools, as target-specific inhibitors.

Research Interests

• 分子生物学
• 細胞生物学
• Molecular Biology
• Cell Biology

Research Areas

• Life sciences, Cell biology
• Life sciences, Molecular biology

Papers

• MONOCLONAL-ANTIBODY SPECIFICALLY REACTING AGAINST 73-KILODALTON HEAT-SHOCK COGNATE PROTEIN - POSSIBLE EXPRESSION ON MAMMALIAN-CELL SURFACE

  N TSUBOI; M ISHIKAWA; Y TAMURA; S TAKAYAMA; H TOBIOKA; A MATSUURA; K HIRAYOSHI; K NAGATA; N SATO; K KIKUCHI

  HYBRIDOMA, Oct. 1994, Peer-reviewed

  
• ANOTHER MECHANISM FOR THE DEFECT IN TYPE-III COLLAGEN ACCUMULATION IN EHLERS-DANLOS SYNDROME TYPE-IV - INCREASED INTRACELLULAR DEGRADATION OF THE PROCOLLAGEN

  A UTANI; T TANAKA; C NISHIGORI; Y MIYACHI; K DANNO; S IMAMURA; M HOSOKAWA; T TAKEDA; K HIRAYOSHI; K NAGATA

  LABORATORY INVESTIGATION, Aug. 1990, Peer-reviewed

  
• Perturbation of discrete sites on a single protein domain with RNA aptamers: Targeting of different sides of the TATA-binding protein (TBP)

  Ken I. Hohmura; Hua Shi; Kazunori Hirayoshi

  Bioscience, Biotechnology and Biochemistry, 2013, Peer-reviewed

  
• Functional analysis of GAGA factor in transcription by RNA aptam

  Hohmuram K; Hirayoshi K

  20th IUBMBinternational Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress 2006 6.18－23 Kyoto, 2006, Peer-reviewed

  
• Cultured murine dermal fibroblast-like cells from senescence-accelerated mice as in vitro models for higher oxidative stress due to mitochondrial alterations

  Y Chiba; Y Yamashita; M Ueno; H Fujisawa; K Hirayoshi; K Hohmura; H Tomimoto; Akiguchi, I; M Satoh; A Shimada; M Hosokawa

  JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, Sep. 2005, Peer-reviewed

  
• Different adaptive traits to cold exposure in young senescence-accelerated mice

  Y Yamashita; Y Chiba; C Xia; K Hirayoshi; M Satoh; Y Saitoh; A Shimada; E Nakamura; M Hosokawa

  BIOGERONTOLOGY, Mar. 2005, Peer-reviewed

  
• Characterization of multiple members of the HSP70 family in platyfish culture cells: Molecular evolution of stress protein HSP70 in vertebrates

  M Yamashita; K Hirayoshi; K Nagata

  GENE, Jul. 2004, Peer-reviewed

  
• Mitochondrial alterations and a higher oxidative status in cultured fibroblast-like cells from senescence-accelerated mice

  Y Chiba; Y Yamashita; K Hirayoshi; M Ueno; H Fujisawa; T Matsushita; K Kogishi; Akiguchi, I; M Satoh; A Shimada; M Hosokawa

  SENESCENCE-ACCELERATED MOUSE (SAM): AN ANIMAL MODEL OF SENESCENCE, 2004, Peer-reviewed

  
• The Kruppel-like factor Zf9 and proteins in the Sp1 family regulate the expression of HSP47, a collagen-specific molecular chaperone

  K Yasuda; K Hirayoshi; H Hirata; H Kubota; N Hosokawa; K Nagata

  JOURNAL OF BIOLOGICAL CHEMISTRY, Nov. 2002, Peer-reviewed

  
• Expression of N-deacetylase/sulfotransferase and 3-O-sulfotransferase in rat alveolar type II cells

  ZY Li; K Hirayoshi; Y Suzuki

  AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, Aug. 2000, Peer-reviewed

  
• Separate cis-acting DNA elements control cell type- and tissue-specific expression of collagen binding molecular chaperone HSP47

  H Hirata; Yamamura, I; K Yasuda; A Kobayashi; N Tada; M Suzuki; K Hirayoshi; N Hosokawa; K Nagata

  JOURNAL OF BIOLOGICAL CHEMISTRY, Dec. 1999, Peer-reviewed

  
• Nuclear run-on assays: Assessing transcription by measuring density of engaged RNA polymerases

  K Hirayoshi; JT Lis

  CHROMATIN, 1999, Peer-reviewed

  
• Direct cloning of DNA that interacts in vivo with a specific protein: application to RNA polymerase II and sites of pausing in Drosophila

  A Law; K Hirayoshi; T O'Brien; JT Lis

  NUCLEIC ACIDS RESEARCH, Feb. 1998, Peer-reviewed

  
• Intracellular interaction of collagen-specific stress protein HSP47 with newly synthesized procollagen

  M Satoh; K Hirayoshi; SI Yokota; N Hosokawa; K Nagata

  JOURNAL OF CELL BIOLOGY, Apr. 1996, Peer-reviewed

  
• Transcriptional regulation of the vimentin-encoding gene in mouse myeloid leukemia M1 cells

  N Nakamura; M Shida; K Hirayoshi; K Nagata

  GENE, Dec. 1995, Peer-reviewed

  
• HSF ACCESS TO HEAT-SHOCK ELEMENTS IN-VIVO DEPENDS CRITICALLY ON PROMOTER ARCHITECTURE DEFINED BY GAGA FACTOR, TFIID AND RNA-POLYMERASE-II BINDING-SITES

  LS SHOPLAND; K HIRAYOSHI; M FERNANDES; JT LIS

  GENES & DEVELOPMENT, Nov. 1995, Peer-reviewed

  
• INTERACTIONS BETWEEN COLLAGEN-BINDING STRESS PROTEIN HSP47 AND COLLAGEN - ANALYSIS OF KINETIC-PARAMETERS BY SURFACE-PLASMON RESONANCE BIOSENSOR

  T NATSUME; T KOIDE; S YOKOTA; K HIRAYOSHI; K NAGATA

  JOURNAL OF BIOLOGICAL CHEMISTRY, Dec. 1994, Peer-reviewed

  
• COEXPRESSION OF THE COLLAGEN-BINDING STRESS PROTEIN HSP47 GENE AND THE ALPHA-1(I) AND ALPHA-1(III) COLLAGEN GENES IN CARBON TETRACHLORIDE-INDUCED RAT-LIVER FIBROSIS

  H MASUDA; M FUKUMOTO; K HIRAYOSHI; K NAGATA

  JOURNAL OF CLINICAL INVESTIGATION, Dec. 1994, Peer-reviewed

  
• PREFERENTIAL LOCALIZATION OF HEAT-SHOCK PROTEIN-47 IN DILATED ENDOPLASMIC-RETICULUM OF CHICKEN CHONDROCYTES

  K KAMBE; A YAMAMOTO; T YOSHIMORI; K HIRAYOSHI; R OGAWA; Y TASHIRO

  JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, Jul. 1994, Peer-reviewed

  
• 2 COLLAGEN-BINDING PROTEINS, OSTEONECTIN AND HSP47, ARE COORDINATELY INDUCED IN TRANSFORMED KERATINOCYTES BY HEAT AND OTHER STRESSES

  H KUDO; K HIRAYOSHI; Y KITAGAWA; S IMAMURA; K NAGATA

  EXPERIMENTAL CELL RESEARCH, Jun. 1994, Peer-reviewed

  
• ENHANCEMENT OF DIFFERENTIATION INDUCTION OF MOUSE MYELOMONOCYTIC LEUKEMIC-CELLS BY EXTRACELLULAR ATP

  M YAMAGUCHI; K HIRAYOSHI; M OKUMA; K NAGATA

  JOURNAL OF CELLULAR PHYSIOLOGY, Jun. 1994, Peer-reviewed

  
• ALTERNATIVE 5' SPLICE-SITE SELECTION INDUCED BY HEAT-SHOCK

  H TAKECHI; N HOSOKAWA; K HIRAYOSHI; K NAGATA

  MOLECULAR AND CELLULAR BIOLOGY, Jan. 1994, Peer-reviewed

  
• Detection of cell surface 73KDa heat shock cognate protein by a newly-developed monoclonal antibody

  平芳 一法

  Hybridoma,13/5,373-381, 1994, Peer-reviewed

  
• STRUCTURE OF THE GENE ENCODING THE MOUSE 47-KDA HEAT-SHOCK PROTEIN (HSP47)

  N HOSOKAWA; H TAKECHI; S YOKOTA; K HIRAYOSHI; K NAGATA

  GENE, Apr. 1993, Peer-reviewed

  
• MODULATION OF THE PHOSPHORYLATION OF GLUCOSE-REGULATED PROTEIN, GRP78, BY TRANSFORMATION AND INHIBITION OF GLYCOSYLATION

  M SATOH; A NAKAI; Y SOKAWA; K HIRAYOSHI; K NAGATA

  EXPERIMENTAL CELL RESEARCH, Mar. 1993, Peer-reviewed

  
• DOWN-REGULATION OF INTERLEUKIN-6 RECEPTORS OF MOUSE MYELOMONOCYTIC LEUKEMIC-CELLS BY LEUKEMIA INHIBITORY FACTOR

  M YAMAGUCHI; M MICHISHITA; K HIRAYOSHI; K YASUKAWA; M OKUMA; K NAGATA

  JOURNAL OF BIOLOGICAL CHEMISTRY, Nov. 1992, Peer-reviewed

  
• QUERCETIN, AN INHIBITOR OF HEAT-SHOCK PROTEIN-SYNTHESIS, INHIBITS THE ACQUISITION OF THERMOTOLERANCE IN A HUMAN COLON-CARCINOMA CELL-LINE

  M KOISHI; N HOSOKAWA; M SATO; A NAKAI; K HIRAYOSHI; M HIRAOKA; M ABE; K NAGATA

  JAPANESE JOURNAL OF CANCER RESEARCH, Nov. 1992, Peer-reviewed

  
• INHIBITION OF THE ACTIVATION OF HEAT-SHOCK FACTOR INVIVO AND INVITRO BY FLAVONOIDS

  N HOSOKAWA; K HIRAYOSHI; H KUDO; H TAKECHI; A AOIKE; K KAWAI; K NAGATA

  MOLECULAR AND CELLULAR BIOLOGY, Aug. 1992, Peer-reviewed

  
• MOLECULAR-CLONING OF A MOUSE 47-KDA HEAT-SHOCK PROTEIN (HSP47), A COLLAGEN-BINDING STRESS PROTEIN, AND ITS EXPRESSION DURING THE DIFFERENTIATION OF F9 TERATOCARCINOMA CELLS

  H TAKECHI; K HIRAYOSHI; A NAKAI; H KUDO; S SAGA; K NAGATA

  EUROPEAN JOURNAL OF BIOCHEMISTRY, Jun. 1992, Peer-reviewed

  
• PURIFICATION AND CHARACTERIZATION OF A VIMENTIN-SPECIFIC PROTEASE IN MOUSE MYELOID-LEUKEMIA CELLS - REGULATION DURING DIFFERENTIATION AND IDENTITY WITH CATHEPSIN-G

  N NAKAMURA; A TSURU; K HIRAYOSHI; K NAGATA

  EUROPEAN JOURNAL OF BIOCHEMISTRY, May 1992, Peer-reviewed

  
• INVOLVEMENT OF THE STRESS PROTEIN HSP47 IN PROCOLLAGEN PROCESSING IN THE ENDOPLASMIC-RETICULUM

  A NAKAI; M SATOH; K HIRAYOSHI; K NAGATA

  JOURNAL OF CELL BIOLOGY, May 1992, Peer-reviewed

  
• QUERCETIN, A BIOFLAVONOID, INHIBITS THE INCREASE OF HUMAN MULTIDRUG RESISTANCE GENE (MDR1) EXPRESSION CAUSED BY ARSENITE

  N KIOKA; N HOSOKAWA; T KOMANO; K HIRAYOSHI; K NAGATA; K UEDA

  FEBS LETTERS, Apr. 1992, Peer-reviewed

  
• EFFECTS OF TYPE-BETA-1 TRANSFORMING GROWTH-FACTOR ON THE PROLIFERATION AND DIFFERENTIATION OF MOUSE MYELOMONOCYTIC LEUKEMIA-CELLS (M1)

  M MICHISHITA; K HIRAYOSHI; A TSURU; N NAKAMURA; Y YOSHIDA; M OKUMA; K NAGATA

  EXPERIMENTAL CELL RESEARCH, Sep. 1991, Peer-reviewed

  
• HSP47 - A TISSUE-SPECIFIC, TRANSFORMATION-SENSITIVE, COLLAGEN-BINDING HEAT-SHOCK PROTEIN OF CHICKEN-EMBRYO FIBROBLASTS

  K HIRAYOSHI; H KUDO; H TAKECHI; A NAKAI; A IWAMATSU; KM YAMADA; K NAGATA

  MOLECULAR AND CELLULAR BIOLOGY, Aug. 1991, Peer-reviewed

  
• BOTH D-FACTOR LIF AND IL-6 INHIBIT THE DIFFERENTIATION OF MOUSE TERATOCARCINOMA F9 CELLS

  K HIRAYOSHI; A TSURU; M YAMASHITA; M TOMIDA; Y YAMAMOTOYAMAGUCHI; K YASUKAWA; M HOZUMI; DV GOEDDEL; K NAGATA

  FEBS LETTERS, May 1991, Peer-reviewed

  
• PHOSPHORYLATION OF THE STRESS PROTEIN HSP27 IS AN EARLY EVENT IN MURINE MYELOMONOCYTIC LEUKEMIC-CELL DIFFERENTIATION INDUCED BY LEUKEMIA INHIBITORY FACTOR D-FACTOR

  M MICHISHITA; M SATOH; M YAMAGUCHI; K HIRAYOSHI; M OKUMA; K NAGATA

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, May 1991, Peer-reviewed

  
• IDENTIFICATION OF THE ATP-BINDING HEAT-INDUCIBLE PROTEIN OF MR=37,000 AS GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE

  A NAKAI; M SATOH; K HIRAYOSHI; K NAGATA

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Apr. 1991, Peer-reviewed

  
• FLAVONOIDS INHIBIT THE EXPRESSION OF HEAT-SHOCK PROTEINS

  N HOSOKAWA; K HIRAYOSHI; A NAKAI; Y HOSOKAWA; N MARUI; M YOSHIDA; T SAKAI; H NISHINO; A AOIKE; K KAWAI; K NAGATA

  CELL STRUCTURE AND FUNCTION, Dec. 1990, Peer-reviewed

  
• NOVEL ATP-BINDING HEAT-INDUCIBLE PROTEIN OF MR = 37,000 THAT IS SENSITIVE TO TRANSFORMATION IN BALB 3T3 CELLS

  A NAKAI; C HIRAYAMA; K OHTSUKA; K HIRAYOSHI; K NAGATA

  JOURNAL OF CELLULAR PHYSIOLOGY, Jun. 1990, Peer-reviewed

  
• REGULATION OF THE EXPRESSION OF VIMENTIN GENE DURING THE DIFFERENTIATION OF MOUSE MYELOID-LEUKEMIA CELLS

  A TSURU; N NAKAMURA; E TAKAYAMA; Y SUZUKI; K HIRAYOSHI; K NAGATA

  JOURNAL OF CELL BIOLOGY, May 1990, Peer-reviewed

  
• TRANSFORMATION OF BALB/3T3 CELLS BY SIMIAN VIRUS-40 CAUSES A DECREASED SYNTHESIS OF A COLLAGEN-BINDING HEAT-SHOCK PROTEIN (HSP47)

  A NAKAI; K HIRAYOSHI; K NAGATA

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jan. 1990, Peer-reviewed

  
• THE TRANSFORMATION-SENSITIVE HEAT-SHOCK PROTEIN (HSP47) BINDS SPECIFICALLY TO FETUIN

  A NAKAI; K HIRAYOSHI; S SAGA; KM YAMADA; K NAGATA

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Oct. 1989, Peer-reviewed

  
• Changes in the intermediate filaments, vimentin, during the differentiation of mouse myeloid leukemia cells

  K. Nagata; A. Tsuru; N. Nakamura; E. Takayama; Y. Suzuki; K. Hirayoshi

  Cell Structure and Function, 1989, Peer-reviewed

  
• BIOSYNTHESIS OF A NOVEL TRANSFORMATION-SENSITIVE HEAT-SHOCK PROTEIN THAT BINDS TO COLLAGEN - REGULATION BY MESSENGER-RNA LEVELS AND INVITRO SYNTHESIS OF A FUNCTIONAL PRECURSOR

  K NAGATA; K HIRAYOSHI; M OBARA; S SAGA; KM YAMADA

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jun. 1988, Peer-reviewed

  
• EFFECT OF THE RECOMBINANT VACCINIA VIRUSES THAT EXPRESS HTLV-I ENVELOPE GENE ON HTLV-I INFECTION

  H SHIDA; T TOCHIKURA; T SATO; T KONNO; K HIRAYOSHI; M SEKI; Y ITO; M HATANAKA; Y HINUMA; M SUGIMOTO; F TAKAHASHINISHIMAKI; T MARUYAMA; K MIKI; K SUZUKI; M MORITA; H SASHIYAMA; M HAYAMI

  EMBO JOURNAL, Nov. 1987, Peer-reviewed

  
• IMMUNOGLOBULIN HEAVY-CHAIN GENE DIVERSIFICATION IN THE LONG-TERM BONE-MARROW CULTURE OF NORMAL MICE AND MICE WITH SEVERE COMBINED IMMUNODEFICIENCY

  K HIRAYOSHI; SI NISHIKAWA; T KINA; M HATANAKA; S HABU; T NOMURA; Y KATSURA

  EUROPEAN JOURNAL OF IMMUNOLOGY, Jul. 1987, Peer-reviewed

  
• EXPRESSION OF THE C-MYC GENE IN HUMAN GASTROINTESTINAL MALIGNANCIES

  K TSUBOI; K HIRAYOSHI; K TAKEUCHI; H SABE; Y SHIMADA; G OHSHIO; T TOBE; M HATANAKA

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Jul. 1987, Peer-reviewed

  
• EXPRESSION OF A PROVIRUS OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I BY DNA TRANSFECTION

  K MORI; H SABE; H SIOMI; T IINO; A TANAKA; K TAKEUCHI; K HIRAYOSHI; M HATANAKA

  JOURNAL OF GENERAL VIROLOGY, Feb. 1987, Peer-reviewed

  
• LIMITING DILUTION ANALYSIS OF THE STEM-CELLS FOR T-CELL LINEAGE

  Y KATSURA; T KINA; T AMAGAI; T TSUBATA; K HIRAYOSHI; Y TAKAOKI; T SADO; SI NISHIKAWA

  JOURNAL OF IMMUNOLOGY, Oct. 1986, Peer-reviewed

  
• GENOMIC EXPRESSIONS OF HUMAN T-LYMPHOTROPIC VIRUS (HTLV-I)

  M HATANAKA; H SABE; A TANAKA; K MORI; H SIOMI; S NAM; Y ADACHI; S ITAMURA; K HIRAYOSHI

  AIDS RESEARCH, Dec. 1986, Peer-reviewed

  

Misc.

• IDENTIFICATION OF CIS-ELEMENTS AND TRANSCRIPTION FACTORS THAT REGULATE THE TISSUE SPECIFIC EXPRESSION OF HSP47

  Yasuda Kunihiko; Hirayoshi Kazunori; Hosokawa Nobuko; Nagata Kazuhiro

  Connective tissue, Apr. 2002

  
• HSP47の組織特異的発現は2つのシスエレメントにより制御されている

  平田普三; 山村功; 安田邦彦; 小林明央; 多田昇弘; 鈴木操; 平芳一法; 細川暢子; 永田和宏

  日本細胞生物学会大会講演要旨集, 1999

  
• コラーゲン特異的分子シャペロンHSP47のプロモーター解析

  平田普三; 山村功; 平芳一法; 細川暢子; 永田和宏

  日本分子生物学会年会プログラム・講演要旨集, Nov. 1998

  
• TRANSCRIPTIONAL CONTROL OF THE EXPRESSION OF NOVEL HEAT-SHOCK PROTEIN (HSP47)

  K NAGATA; K HIRAYOSHI; S SAGA; M OBARA; KM YAMADA

  CELL STRUCTURE AND FUNCTION, Dec. 1987

  
• DIFFERENTIATION OF THE TERATOCARCINOMA CELL F9 AND THE INDUCTION OF HEAT-SHOCK PROTEIN (HSP47)

  K HIRAYOSHI; K NAGATA

  CELL STRUCTURE AND FUNCTION, Dec. 1987

  
• 転写開始複合体形成におけるクロマチンリモデリング因子の関与

  法邑賢一; 平芳一法

  ポスター3 転写開始複合体形成におけるクロマチンリモデリング因子の関与 法邑賢一、平芳一法（細胞機能調節学分野） 「再生医学・再生医療の先端融合的共同研究拠点」 平成24 年度学術講演会 開催日：2012 年12 月19 日（水）, 2012, Peer-reviewed

  
• RNA アプタマーを用いたGAF の機能解析

  法邑賢一; 平芳一法

  第53 回日本分子生物学会物学会年会， （2012.12.11－14．福岡）, 2012, Peer-reviewed

  
• 転写複合体形成過程におけるTBP/GAF 間相互作用の解析

  平芳一法; 法邑賢一

  第84 回日本遺伝学会大会 （2012.9.24－26．福岡）, 2012, Peer-reviewed

  
• Analyses of communication between GAF and TBP by RNA aptamers

  法邑 賢一; 平芳 一法

  京都大学再生医科学研究所 「再生医学・再生医療の先端融合的共同研究拠点」 平成23 年度 学術講演会 2011 年12 月26 日（月） ポスター3, 2011, Peer-reviewed

  
• 転写初期段階におけるクロマチン関連因子を核とした因子間ネットワークの解明

  法邑賢一; 平芳一法

  京都大学再生医科学研究所 \n「再生医学・再生医療の先端融合的共同研究拠点」 \n平成22 年度学術講演会 \n開催日：2010 年12 月13 日（月） \n場所：芝蘭会館稲盛ホール, 2010, Peer-reviewed

  
• PIC 形成過程におけるTBP/GAF 間相互作用の役割

  法邑賢一; 平芳一法

  ．第32 回日本分子生物学会年会 （2009.12.9－12．横浜）, 2009, Peer-reviewed

  
• RNA アプタマーを用いた転写因子GAF の機能解析

  法邑賢一; 平芳一法

  第82 回日本生化学会大会（2009.10.21－ 24．神戸）, 2009, Peer-reviewed

  
• RNA APTAMER AS A MOLECULAR TOOL FOR ANALYZING THE PROTEIN-PROTEIN INTERACTION

  Ken-ichi Hohmura; Kazunori Hirayoshi

  POSTER 12 International Symposium on Regenerative Medical Therapy (Institute for Frontier Medical Sciences, Kyoto University 2007 年9 月19 日（水）20 日（木）, 2007, Peer-reviewed

  
• 転写開始後におけるGAGA 因子の機能解析の解析

  法邑賢一; 平芳一法

  第30 回日本分子生物学会物学会年会 （2007.12.11.～15. 横浜）, 2007, Peer-reviewed

  
• The analysis of intra-molecular regulation of TBP by RNA aptamer

  平芳一法; 法邑賢一

  第78 回日本生化学会大会 ワークショップ「転写I」（2005. 10. 20．神戸）, 20 Oct. 2005, Peer-reviewed

  
• Involvements of GAGA factor in transcription analyzed by RNA aptamers

  法邑賢一; 平芳一法

  第78 回日本生化学会大会ワークショップ「転写I」（2005. 10. 20．神戸）, 20 Oct. 2005, Peer-reviewed

  
• TBP 特異的RNA アプタマーを用いた転写機構の解析

  平芳一法; 法邑賢一

  第28 回日本分子生物学会年会（2005. 12. 7. ～10．福岡）, 2005, Peer-reviewed

  
• 転写過程におけるGAGA 因子複合体の機能―RNA アプタマーを用いた解析―

  邑賢一; 平芳一法

  第28 回日 本分子生物学会年会(2005. 12. 7. ～10．福岡）, 2005, Peer-reviewed

  
• The analysis of transcription complex by RNA aptamer（1）Drosophila TBP as a target

  法邑賢一; 平芳一法

  第77 回 日本生化学会大会（2004.10.13.～16. 横浜）, 13 Oct. 2004, Peer-reviewed

  
• TBP 特異的RNA アプタマーを用いた転写調節機構の解析

  法邑賢一; 平芳一法

  第27 回日本分子生物学会年会 （2004.12.8.～11. 神戸）, 2004, Peer-reviewed

  
• ショウジョウバエGAGA 因子に結合するRNA アプタマーの解析

  平芳一法; 法邑賢一

  第27 回日本分子生物学会年会（2004.12.8.～11. 神戸）, 2004, Peer-reviewed

  
• RNA aptqamerによる転写複合体中因子の機能解析

  法邑 賢一; 平芳 一法

  第37回日本分子生物学会年会 (2014.11.25-27. 横浜

  

Presentations

• I型コラーゲンに対するRNAアプタマーの選別

  平芳 一法

  第41回日本分子生物学会年会, 28 Nov. 2018

  
• RNAアプタマーによるショウジョウバエ転写因子GAFの機能解析

  平芳 一法

  06 Dec. 2017

  

External funds: Kakenhi

• Selection of RNA aptamer against collagen and apply to new drug discovery

  Grant-in-Aid for Challenging Research (Exploratory)

  Kyoto University

  Kazunori Hirayoshi

  From 30 Jun. 2017, To 31 Mar. 2019, Project Closed

  RNAアプタマー;コラーゲン;RNA aptamer

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K19484/
• Transplantation of co-aggregates of islet cells and immune suppressor cells

  Grant-in-Aid for Scientific Research (A)

  Kyoto University

  Hiroo IWATA

  From 01 Apr. 2012, To 31 Mar. 2015, Project Closed

  ランゲルハンス氏島;膵島;制御性T細胞;糖尿病;血糖値;移植;皮下移植;拒絶反応

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24240078/
• The analysis of general transcription machinary by RNA aptamer

  Grant-in-Aid for Scientific Research (C)

  KYOTO UNIVERSITY

  Kazunori HIRAYOSHI

  Project Closed

  RNA aptamer;初期転写複合体;表面プラズモン共鳴;SELEX;TBP;Paused polymerase;基本転写因子;TAF60;ショウジョウバエ, RNA aptamer;pre-initiation complex;surface plasmon resonance;SELEX;TBP

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680673/
• Regulation of phenotype and gene expression in alveolar epithelial cells

  Grant-in-Aid for Scientific Research (C)

  KYOTO UNIVERSITY

  Yasuhiro SUZUKI

  Project Closed

  肺胞II型細胞;分化転換;硫酸転移酵素;プロテオグリカン;細胞伸展;肺障害;MT-MMP;肺傷害;肺胞上皮細胞;遺伝子発現;ヘパラン硫酸;ハービマイシンA;リチウム, type II cell;transdifferentiation;sulfotransferase;proteoglycan;cell spreading;lung damage;MT-MMP

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670201/
• コラーゲン特異的分予シャペロンHSP47の構造と機能

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  永田 和宏

  Project Closed

  HSP47;コラーゲン;semi-permeabilized cell;ノックアウトマウス;分子シャペロン;結晶解析

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11694271/
• The analysis of the function and establishment mechnism of paused polymerase

  Grant-in-Aid for Scientific Research (C)

  KYOTO UNIVERSITY

  Kazunori HIRAYOSHI

  Project Closed

  転写調節;ショウジョウバエ;RNA polymerase II;Paused polymerase;HSP70;TATA;DPE;GAGA;GAGA因子, Transcriptional regulation;Drosophila;RNA polymerasell;Paused polymerase;HSP70;TATA;DPE;GAGA

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680650/
• ストレス蛋白質HSP47の転写調節機構の解明

  Grant-in-Aid for General Scientific Research (C)

  Kyoto University

  平芳 一法

  Project Closed

  ストレス蛋白質;コラーゲン;transtection

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05680610/
• コラーゲン特異的ストレス蛋白質HSP47の機能と発現

  Grant-in-Aid for Scientific Research on Priority Areas

  Kyoto University

  平芳 一法

  Project Closed

  ストレス蛋白質;Breferdin A;Monensin;Bafilomycin A1;コラーゲン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05252219/
• Studies on the molecular mechanism of heat shock protein 47.

  Grant-in-Aid for Overseas Scientific Survey.

  Kyoto University

  Akira NAKAI

  Project Closed

  熱ショック蛋白質;ストレス蛋白質;細胞内輸送;コラーゲン;分子シャペロン;構造解析;分子間結合;分子生物学, Heat Shock Protein;Stress Protein;Molecular Chaperone;Structual Analysis;Collagen;Molecular Biology;Intracellnlar transport;Intermolecular Association

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05044215/
• コラーゲン特異的ストレス蛋白質HSP47の発現調節

  Grant-in-Aid for General Scientific Research (C)

  Kyoto University

  平芳 一法

  Project Closed

  ストレス蛋白質;コラーゲン;分子シャペロン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04833014/
• The role of stress proteins in the protein biosynthesis.

  Grant-in-Aid for international Scientific Research

  Kyoto University

  Kazuhiro NAGATA

  Project Closed

  ストレス蛋白質;熱ショック転写因子;分子シャペロン;コラーゲン;小胞体, Stress protein;Heat shock transcription factor;Molecular Chaperone

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04044100/
• コラ-ゲン特異的ストレス蛋白質HSP47の構造と機能

  Grant-in-Aid for General Scientific Research (C)

  Kyoto University

  平芳 一法

  Project Closed

  ストレス蛋白質;コラ-ゲン;分子シャペロン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03833017/
• 温熱耐性克服のための熱ショック蛋白質の基礎的研究

  Grant-in-Aid for Cancer Research

  Kyoto University

  永田 和宏

  Project Closed

  hsp47;熱ショック蛋白質(ヒートショックプロテイン);トランスフォーメーション;コラーゲン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63015046/

External funds: others

• The function and regulation of collagen specific shaperone HSP47
• The function and mechanism of generating paused polymerase
• The analysis of transcription mechanism by RNA aptamer

Education

Teaching subject(s)

• From Apr. 2012, To Mar. 2013

  gene expression101

  Spring, 全学共通科目
• From Apr. 2013, To Mar. 2014

  gene expression101

  Spring, 全学共通科目
• From Apr. 2014, To Mar. 2015

  gene expression101

  Spring, 全学共通科目
• From Apr. 2015, To Mar. 2016

  gene expression 101

  Spring, 全学共通科目
• From Apr. 2016, To Mar. 2017

  ILAS Seminar

  Spring, 全学共通科目
• From Apr. 2017, To Mar. 2018

  ILAS Seminar

  Spring, 全学共通科目
• From Apr. 2018, To Mar. 2019

  ILAS Seminar

  Spring, 全学共通科目
• From Apr. 2019, To Mar. 2020

  ILAS Seminar

  Spring, 全学共通科目
• From Apr. 2020, To Mar. 2021

  ILAS Seminar :gene expression 101 [How do we regulate the gene expression?]

  Spring, 全学共通科目
• From Apr. 2021, To Mar. 2022

  ILAS Seminar :gene expression 101 [How do we regulate the gene expression?]

  Spring, 全学共通科目

Part-time lecturer

• From 01 Apr. 2019, To 22 Sep. 2019

  京都光華女子大学

Administration

School management (title, position)

• 放射線障害予防小委員会 委員
• From 01 Apr. 2016, To 31 Mar. 2018

  有機廃液情報管理小委員会委員
• 特別管理産業廃棄物管理責任者

Faculty management (title, position)

• To 01 May 2021

  共同利用機器等委員会　委員
• From 01 Oct. 2016

  化学物質管理委員会　委員
• From 01 Jun. 2017

  施設整備委員会　委員
• To 30 Apr. 2021

  共同利用機器等委員会 委員長
• 安全衛生委員会　委員
• 図書委員会　委員
• To 31 Mar. 2023

  医学図書館連絡委員
• From 01 Oct. 2016, To Sep. 2019

  放射線障害防止委員会　委員
• 共同機器室運営委員会　委員
• From 01 Oct. 2016

  （旧再生研）共同機器室運営委員会　委員長