Regulation of carilage and bone formation and their regeneration
Overview of the research
The treatment of cartilage is a challenging issue. We are investigating mechanisms regulating the formation and differentiation of cartilage. We are also going to analyze cause of cartilage diseases and to develop a method to regenerate cartilage.
Research Interests
整形外科
軟骨代謝
Regenerative medicine
Research Areas
Life sciences, Functional biochemistry
Papers
Human iPS cell-derived cartilaginous tissue spatially and functionally replaces nucleus pulposus
Stem cell aging and the implications for stem cell-based therapies for aging-related diseases and aged tissues
Fujita K; Tsumaki N
Clin Calcium., Jan. 2013, Invited
Osterix Regulates Calcification and Degradation of Chondrogenic Matrices through Matrix Metalloproteinase 13 (MMP13) Expression in Association with Transcription Factor Runx2 during Endochondral Ossification
Wwp2 is essential for palatogenesis mediated by the interaction between Sox9 and mediator subunit 25
Yukio Nakamura; Koji Yamamoto; Xinjun He; Bungo Otsuki; Youngwoo Kim; Hiroki Murao; Tsunemitsu Soeda; Noriyuki Tsumaki; Jian Min Deng; Zhaoping Zhang; Richard R. Behringer; Benoit de Crombrugghe; John H. Postlethwait; Matthew L. Warman; Takashi Nakamura; Haruhiko Akiyama
NATURE COMMUNICATIONS, Mar. 2011, Peer-reviewed
Identification of small molecular compounds and fabrication of its aqueous solution by laser-ablation, expanding primordial cartilage
D. Ikegami; T. Iwai; S. Ryo; N. Gu; T. Sugiyama; I. Oh; H. Yoshikawa; N. Tsumaki
OSTEOARTHRITIS AND CARTILAGE, Feb. 2011, Peer-reviewed
Generation of hyaline cartilaginous tissue from mouse adult dermal fibroblast culture by defined factors
Identification of an enhancer sequence within the first intron required for cartilage-specific transcription of the alpha 2(XI) collagen gene. (vol 275, pg 12712, 2000)
Low-intensity pulsed ultrasound accelerates maturation of callus in patients treated with opening-wedge high tibial osteotomy by hemicallotasis
N Tsumaki; M Kakiuchi; J Sasaki; T Ochi; H Yoshikawa
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, Nov. 2004, Peer-reviewed
Repair of articular cartilage defects in rabbits using CDMP1 gene-transfected autologous mesenchymal cells derived from bone marrow
R Katayama; S Wakitani; N Tsumaki; Y Morita; Matsushita, I; R Gejo; T Kimura
RHEUMATOLOGY, Aug. 2004, Peer-reviewed
[Role of BMPs and Smads during endochondral bone formation].
Tsumaki N; Horiki M; Murai J; Yoshikawa H
Clinical calcium, Jul. 2004, Peer-reviewed
Smad6/Smurf1 overexpression in cartilage delays chondrocyte hypertrophy and causes dwarfism with osteopenia
M Horiki; T Imamura; M Okamoto; M Hayashi; J Murai; A Myoui; T Ochi; K Miyazono; H Yoshikawa; N Tsumaki
JOURNAL OF CELL BIOLOGY, May 2004, Peer-reviewed
COL11A2 collagen gene transcription is differentially regulated by EWS/ERG sarcoma fusion protein and wild-type ERG
Y Matsui; HA Chansky; F Barahmand-Pour; A Zielinska-Kwiatkowska; N Tsumaki; A Myoui; H Yoshikawa; L Yang; DR Eyre
JOURNAL OF BIOLOGICAL CHEMISTRY, Mar. 2003, Peer-reviewed
Cartilage-specific overexpression of C-propeptide of type II collagen affected matrix mineralization in transgenic mice
Y. Matsui; K. Nakata; E. Adachi; N. Tsumaki; T. Kimura; N. Yasui; E. Kataoka; Y. Fujita; H. Tsuboi; H. Yoshikawa
Journal of Musculoskeletal Research, 2003, Peer-reviewed
Differential expression of aggrecan mRNA isoforms by chondrosarcoma cells
Y Matsui; N Araki; H Tsuboi; N Tsumaki; K Nakata; H Kawabata; DR Eyre; H Yoshikawa
ANTICANCER RESEARCH, Nov. 2002, Peer-reviewed
Bone morphogenetic protein signals are required for cartilage formation and differently regulate joint development during skeletogenesis
N Tsumaki; T Nakase; T Miyaji; M Kakiuchi; T Kimura; T Ochi; H Yoshikawa
JOURNAL OF BONE AND MINERAL RESEARCH, May 2002, Peer-reviewed
Activation of cartilage-derived morphogenetic protein-1 in torn rotator cuff
Takanobu Nakase; Kazuomi Sugamoto; Takashi Miyamoto; Noriyuki Tsumaki; Frank P. Luyten; Hiroaki Inui; Akira Myoui; Tetsuya Tomita; Hideki Yoshikawa
Clinical Orthopaedics and Related Research, 2002, Peer-reviewed
Activation and localization of cartilage-derived morphogenetic protein-1 at the site of ossification of the ligamentum flavum
T Nakase; K Ariga; K Yonenobu; N Tsumaki; FP Luyten; Y Mukai; Sato, I; H Yoshikawa
EUROPEAN SPINE JOURNAL, Aug. 2001, Peer-reviewed
Alternative splicing of fibronectin transcripts in osteochondrogenic tumors
Y Matsui; K Nakata; N Araki; K Ozono; Y Fujita; N Tsumaki; H Kawabata; N Yasui; K Sekiguchi; H Yoshikawa
ANTICANCER RESEARCH, Mar. 2001, Peer-reviewed
Identification of an enhancer sequence within the first intron required for cartilage-specific transcription of the alpha 2(XI) collagen gene
Y Liu; HC Li; K Tanaka; N Tsumaki; Y Yamada
JOURNAL OF BIOLOGICAL CHEMISTRY, Apr. 2000, Peer-reviewed
Enhancer analysis of the alpha 1(II) and alpha 2(XI) collagen genes in transfected chondrocytes and transgenic mice.
N. Tsumaki; Y. Liu; Y. Yamada; P. Krebsbach
Methods in molecular biology (Clifton, N.J.), 2000, Peer-reviewed
A novel type X collagen gene mutation (G595R) associated with Schmid-type metaphyseal chondrodysplasia
Y Matsui; N Yasui; H Kawabata; K Ozono; K Nakata; T Mizushima; N Tsumaki; E Kataoka; Y Fujita; T Ochi
JOURNAL OF HUMAN GENETICS, 2000, Peer-reviewed
Role of CDMP-1 in skeletal morphogenesis: Promotion of mesenchymal cell recruitment and chondrocyte differentiation
N Tsumaki; K Tanaka; E Arikawa-Hirasawa; T Nakase; T Kimura; JT Thomas; T Ochi; FP Luyten; Y Yamada
JOURNAL OF CELL BIOLOGY, Jan. 1999, Peer-reviewed
Genotype phenotype correlation in achondroplasia and hypochondroplasia
Y Matsui; N Yasui; T Kimura; N Tsumaki; H Kawabata; T Ochi
JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME, Nov. 1998, Peer-reviewed
Modular arrangement of cartilage- and neural tissue-specific cis-elements in the mouse alpha 2(XI) collagen promoter
N Tsumaki; T Kimura; K Tanaka; JH Kimura; T Ochi; Y Yamada
JOURNAL OF BIOLOGICAL CHEMISTRY, Sep. 1998, Peer-reviewed
Differential in situ expression of alpha 2(XI) collagen mRNA isoforms in the developing mouse
M Sugimoto; T Kimura; N Tsumaki; Y Matsui; K Nakata; H Kawahata; N Yasui; Y Kitamura; S Nomura; T Ochi
CELL AND TISSUE RESEARCH, May 1998, Peer-reviewed
Splicing patterns of type XI collagen transcripts act as molecular markers for osteochondrogenic tumors
Y Matsui; T Kimura; N Tsumaki; K Nakata; N Yasui; N Araki; N Hashimoto; A Uchida; T Ochi
CANCER LETTERS, Feb. 1998, Peer-reviewed
A recurrent 1992delCT mutation of the type X collagen gene in a Japanese patient with Schmid metaphyseal chondrodysplasia
Y Matsui; T Kimura; N Tsumaki; N Yasui; T Ochi
JAPANESE JOURNAL OF HUMAN GENETICS, Sep. 1996, Peer-reviewed
Separable cis-regulatory elements that contribute to tissue- and site-specific alpha 2(XI) collagen gene expression in the embryonic mouse cartilage
N Tsumaki; T Kimura; Y Matsui; K Nakata; T Ochi
JOURNAL OF CELL BIOLOGY, Sep. 1996, Peer-reviewed
Cervical spondylotic radiculopathy involving two adjacent nerve roots - Anterior decompression through a single level intervertebral approach
M Iwasaki; K Okada; N Tsumaki; H Obata; N Shirasaki; S Oka
INTERNATIONAL ORTHOPAEDICS, Jun. 1996, Peer-reviewed
Progressive degeneration of articular cartilage and intervertebral discs - An experimental study in transgenic mice bearing a type IX collagen mutation
T Kimura; K Nakata; N Tsumaki; S Miyamoto; Y Matsui; S Ebara; T Ochi
INTERNATIONAL ORTHOPAEDICS, Jun. 1996, Peer-reviewed
Separable regulatory elements from pro-alpha 2(XI) collagen gene drive distinct patterns of cartilage-specific expression in transgenic mice
N Tsumaki; M Sugimoto; K Nakata; Y Matsui; T Ochi; T Kimura
MOLECULAR AND DEVELOPMENTAL BIOLOGY OF CARTILAGE, 1996, Peer-reviewed
DIFFERENTIAL EXPRESSION OF AN ACIDIC DOMAIN IN THE AMINO-TERMINAL PROPEPTIDE OF MOUSE PRO-ALPHA-2(XI) COLLAGEN BY COMPLEX ALTERNATIVE SPLICING
N TSUMAKI; T KIMURA
JOURNAL OF BIOLOGICAL CHEMISTRY, Feb. 1995, Peer-reviewed
ALTERED CHONDROCYTE GENE-EXPRESSION IN ARTICULAR-CARTILAGE MATRIX COMPONENTS AND SUSCEPTIBILITY TO CARTILAGE DESTRUCTION
T KIMURA; K NAKATA; N TSUMAKI; K ONO
CELL MECHANICS AND CELLULAR ENGINEERING, 1994, Peer-reviewed
Misc.
Effect of low-intensity pulsed ultrasound on maturation of callus in opening-wedge high tibial osteotomy by hemicallotasis
妻木 範行; TANAKA K.; ARIKAWA HIRASAWA E.; THOMAS J. T.; 越智 隆弘; LUYTEN F. P.; 木村 友厚; YAMADA Y.
日本骨代謝学会雑誌 = Japanese journal of bone metabolism, 07 Jul. 1998
マウスXI型コラーゲンα2鎖遺伝子の軟骨特異的発現にかかわる転写制御領域の解析
妻木 範行; 木村 友厚; 松井 好人; 杉本 瑞生; 中田 研; 越智 隆弘
日本骨代謝学会雑誌 = Japanese journal of bone metabolism, 01 Jun. 1996
Role of CDMP-1 in Skeletal Morphogenesis : Promotion of Mesenchymal Cell Recruitment and Chondrocyte Differentiation
TSUMAKI N.; THOMAS J. T.; LUYTEN F. P.; YAMADA Y.
日本整形外科學會雜誌 = The Journal of the Japanese Orthopaedic Association, 25 Aug. 1998
Paediatric and Adolescent Throwing Injury : The Actual Conditions and Its Prevention
MASATOMI T.
日本整形外科學會雜誌 = The Journal of the Japanese Orthopaedic Association, 25 Feb. 1995
Identification of the regulatory sequences responsible for cartilage-specific expression of the Col11a2 gene and their application : a role of BMP signals in the skeletal development
Tsumaki N.; Kimura T.; Yamada Y.; Ochi T.
Connective tissue, Jun. 1999
Targeted expression of CDMP-1 in mouse cartilage expands primordial cartilage and enhances chondrocyte differentiation
Tsumaki N.; Tanaka K.; Akikawa-Hirasawa E.; Thomas J.T.; Ochi T.; Luyten F.P.; Kimura T.; Yamada Y.
Connective tissue, 05 Jun. 1998
BMP signals are required for cartilage formation.
Tsumaki N.; Nakase T.; Miyaji E. T.; Kakiuchi M.; Ochi T.; Yamada Yoshihiko; Kimura T.; Yoshikawa H.
Connective tissue, Jun. 2001
軟骨変性と遺伝子の発現 -軟骨細胞signaling pathway における意味づけ-
妻木 範行; 垣内 雅明; 吉川 秀樹; 木村 友厚; 越智 隆弘
リウマチ, 26 Apr. 2000
軟骨特異的転写制御メカニズムの解明とその応用
妻木 範行; 木村 友厚; YAMADA Yoshihiko; 越智 隆弘
リウマチ, 30 Apr. 1999
Role of BMP4 during Chondrocyte Differentiation
TSUMAKI N.
日本整形外科學會雜誌, 25 Aug. 2000
Transcriptional Regulation of Cartilage-specific Collagen Gene, Col11a2
Directed induction of chondrogenic cells from mouse dermal fibroblast culture
Noriyuki Tsumaki
ARTHRITIS RESEARCH & THERAPY, 2012
A GAIN-OF-FUNCTION TYPE MUTATION OF THE NATRIURETIC PEPTIDE RECEPTOR B CAUSES ACCELERATION OF SKELETAL GROWTH AND OSTEOPOROTIC CHANGE IN HUMANS AND MICE
K. Miura; N. Namba; M. Fujiwara; T. Kitaoka; Y. Ohata; H. Hirai; C. Higuchi; N. Tsumaki; H. Yoshikawa; T. Michigami; K. Ozono
Regulation of the col11a2 expression through the histone modification.
J. Murai; H. Yoshikawa; N. Tsumaki
JOURNAL OF BONE AND MINERAL RESEARCH, Sep. 2006
Role of Smad7 during endochondral bone formation.
T. Iwai; J. Murai; H. Yoshikawa; N. Tsumaki
JOURNAL OF BONE AND MINERAL RESEARCH, Sep. 2006
Bone morphogenetic proteins in bone stimulate osteoclasts and osteoblasts during bone development
M Okamoto; J Murai; H Yoshikawa; N Tsumaki
JOURNAL OF BONE AND MINERAL RESEARCH, Jul. 2006
Identification of an enhancer sequence within the first intron required for cartilage-specific transcription of the alpha 2(XI) collagen gene (vol 275, pg 12712, 2000)
Low-intensity pulsed ultrasound accelerates maturation of callus in patients treated with opening-wedge high tibial osteotomy by hemicallotasis
N Tsumaki; M Kakiuchi; J Sasaki; T Ochi; H Yoshikawa
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, Nov. 2004
Low-intensity pulsed ultrasound accelerates maturation of callus in patients treated with opening-wedge high tibial osteotomy by hemicallotasis
N Tsumaki; M Kakiuchi; J Sasaki; T Ochi; H Yoshikawa
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, Nov. 2004
Hypoxia promotes chondrocytic differentiation in vitro and cartilaginous matrix synthesis in organ culture via p38 kinase pathway
M Hirao; N Tamai; N Tsumaki; A Myoui; H Yoshikawa
JOURNAL OF BONE AND MINERAL RESEARCH, Oct. 2004
Repair of articular cartilage defects in rabbits using CDMP1 gene-transfected autologous mesenchymal cells derived from bone marrow
R Katayama; S Wakitani; N Tsumaki; Y Morita; Matsushita, I; R Gejo; T Kimura
RHEUMATOLOGY, Aug. 2004
Repair of articular cartilage defects in rabbits using CDMP1 gene-transfected autologous mesenchymal cells derived from bone marrow
R Katayama; S Wakitani; N Tsumaki; Y Morita; Matsushita, I; R Gejo; T Kimura
RHEUMATOLOGY, Aug. 2004
Smad6/Smurf1 overexpression in cartilage delays chondrocyte hypertrophy and causes dwarfism with osteopenia
M Horiki; T Imamura; M Okamoto; M Hayashi; J Murai; A Myoui; T Ochi; K Miyazono; H Yoshikawa; N Tsumaki
JOURNAL OF CELL BIOLOGY, May 2004
Smad6/Smurf1 overexpression in cartilage delays chondrocyte hypertrophy and causes dwarfism with osteopenia
M Horiki; T Imamura; M Okamoto; M Hayashi; J Murai; A Myoui; T Ochi; K Miyazono; H Yoshikawa; N Tsumaki
JOURNAL OF CELL BIOLOGY, May 2004
Overexpression of BMP4 in osteoblasts causes osteopenia in transgenic mice
J Murai; N Tsumaki; M Horiki; Y Hashimoto; K Kuriyama; H Yoshikawa
BONE, 2004
Hypoxia promotes chondrocytic differentiation of C3H10T1/2 cells induced by bone morphogenetic protein-2
M Hirao; N Tamai; N Tsumaki; A Myoui; H Yoshikawa
BONE, 2004
Overexpression of BMP4 in osteoblasts causes severe osteopenia in transgenic mice.
N Tsumaki; M Horiki; Y Hashimoto; K Kuriyama; H Yoshikawa
JOURNAL OF BONE AND MINERAL RESEARCH, Sep. 2003
Overexpression of Smad6 in chondrocytes distinctively disturbs terminal differentiation of chondrocytes and causes osteopenia in transgenic mice.
M Horiki; N Tsumaki; A Myoui; T Imamura; H Yoshikawa
JOURNAL OF BONE AND MINERAL RESEARCH, Sep. 2003
Role of Smad6 during cartilage formation
M Horiki; N Tsumaki; A Myoui; T Miyaji; T Imamura; H Yoshikawa
BONE, May 2003
COL11A2 collagen gene transcription is differentially regulated by EWS/ERG sarcoma fusion protein and wild-type ERG
Y Matsui; HA Chansky; F Barahmand-Pour; A Zielinska-Kwiatkowska; N Tsumaki; A Myoui; H Yoshikawa; L Yang; DR Eyre
JOURNAL OF BIOLOGICAL CHEMISTRY, Mar. 2003
Role of bone morphogenetic protein signals during skeletal development
N Tsumaki; M Horiki; T Kimura; H Yoshikawa
ARTHRITIS RESEARCH & THERAPY, 2003
Repair of rabbit articular cartilage defect using CDMP1-transfected autologous mesenchymal stem cell derived from bone marrow.
R Katayama; S Wakitani; N Tsumaki; H Matsuno; L Frank; K Yudoh; T Kimura
ARTHRITIS AND RHEUMATISM, Sep. 2002
A Kruppel-associated box-zinc finger protein, NT2, represses cell-type-specific promoter activity of the alpha 2(XI) collagen gene (Retracted Article)
K Tanaka; N Tsumaki; CA Kozak; Y Matsumoto; F Nakatani; Y Iwamoto; Y Yamada
MOLECULAR AND CELLULAR BIOLOGY, Jun. 2002
Bone morphogenetic protein signals are required for cartilage formation and differently regulate joint development during skeletogenesis
N Tsumaki; T Nakase; T Miyaji; M Kakiuchi; T Kimura; T Ochi; H Yoshikawa
JOURNAL OF BONE AND MINERAL RESEARCH, May 2002
Matsui, Y., Araki, N., Tsuboi, H., Tsumaki, N., Nakata, K., Kawabata, H., Eyre, D. R., and Yoshikawa, H.: Differential expression of aggrecan mRNA isoforms by chondrosarcoma cells.
Anticancer Res, 2002
Nakase, T., Sugamoto, K., Miyamoto, T., Tsumaki, N., Luyten, F. P., Inui, H., Myoui, A., Tomita, T., and Yoshikawa, H.: Activation of cartilage-derived morphogenetic protein-1 in torn rotator cuff.
Clin Orthop, 2002
Activation and localization of cartilage-derived morphogenetic protein-1 at the site of ossification of the ligamentum flavum
T Nakase; K Ariga; K Yonenobu; N Tsumaki; FP Luyten; Y Mukai; Sato, I; H Yoshikawa
EUROPEAN SPINE JOURNAL, Aug. 2001
Flagエピトープ標識II型コラーゲンCプロペプチド過剰発現マウスにおける外来遺伝子発現の免疫組織学的検索(Demonstration of type II collagen C-propeptide tagged with Flag epitope (DYKDDDDK) in cartilage of transgenic mice)
Matsui, Y., Nakata, K., Araki, N., Ozono, K., Fujita, Y., Tsumaki, N., Kawabata, H., Yasui, N., Sekiguchi, K., and Yoshikawa, H.: Alternative splicing of fibronectin transcripts in osteochondrogenic tumors.
Anticancer Res, 2001
Involvement of Ptc/Gli-BMP signaling in the Periosteum in the Early Phase of Fracture Repair
NAKASE T.
日本整形外科學會雜誌, 25 Aug. 2000
Identification of an enhancer sequence within the first intron required for cartilage-specific transcription of the alpha 2(XI) collagen gene
Y Liu; HC Li; K Tanaka; N Tsumaki; Y Yamada
JOURNAL OF BIOLOGICAL CHEMISTRY, Apr. 2000
A novel type X collagen gene mutation (G595R) associated with Schmid-type metaphyseal chondrodysplasia
Y. Matsui; N. Yasui; H. Kawabata; K. Ozono; K. Nakata; T. Mizushima; N. Tsumaki; E. Kataoka; Y. Fujita; T. Ochi
Journal of Human Genetics, Mar. 2000
Tsumaki, N., Liu, Y., Yamada, Y., and Krebsbach, P.: Enhancer analysis of the 1(II) and 2(XI) collagen genes in transfected chondrocytes and transgenic mice.
Methods Mol Biol, 2000
Targeted gene expression in human articular cartilage by direct in vivo gene transfer into SCID-HuRAg mouse.
R Katayama; T Tomita; N Tsumaki; T Ochi; H Matsuno; Y Morita; K Yudoh; F Nakazawa; T Kimura
日本骨代謝学会雑誌 = Japanese journal of bone metabolism, 01 Jun. 1996
PROGRESSIVE DEGENERATION OF ARTICULAR-CARTILAGE AND INTERVERTEBRAL DISCS IN TRANSGENIC MICE BEARING A TYPE-IX COLLAGEN MUTATION
T KIMURA; K NAKATA; N TSUMAKI; Y MATSUI; T OCHI
ARTHRITIS AND RHEUMATISM, Sep. 1995
Tsumaki, N., and Kimura, T.: Differential expression of an acidic domain in the amino-terminal propeptide of mouse pro-2(XI) collagen by complex alternative splicing.
J Biol Chem, 1995
骨軟骨コラーゲン異常モデルマウス
中田 研; 妻木 範行; 木村 友厚
Molecular Medicine, Dec. 1994
Works
厚生労働科学研究費補助金 免疫アレルギー疾患予防・治療研究事業
From 2006
超音波刺激,人工骨,骨形成因子(BMP)シグナル操作を組み合わせた骨欠損の修復
From 2006
軟骨細胞シグナリングとマトリックス転写制御による軟骨形成・分化の解明
From 2006
難治性骨折治癒過程におけるBMPシグナル作用機構の解明
From 2005
list
Last Updated :2022/08/06
Education
Teaching subject(s)
From Apr. 2020, To Mar. 2021
Frontier in Life Sciences
Fall, 全学共通科目
From Apr. 2020, To Mar. 2021
Frontier in Life Sciences
Fall, 生命科学研究科
Participation in PhD Defense
In vivo regeneration of rat laryngeal cartilage with mesenchymal stem cells derived from human induced pluripotent stem cells via neural crest cells
YOSHIMATSU MASAYOSHI, Graduate School of Medicine, Sub-chief Examiner
26 Jul. 2021
Characterization of hiPSC-Derived Muscle Progenitors Reveals Distinctive Markers for Myogenic Cell Purification Toward Cell Therapy
HARUTIUN MINAS NALBANDIAN GEYMONAT, Graduate School of Medicine, Chief Examiner