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Usukura, Eiji

Graduate School of Medicine, Medicine Program-Specific Assistant Professor

Usukura, Eiji
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    Last Updated :2022/06/30

    Basic Information

    Academic Degree

    • 修士(工学)(東京農工大学)
    • 博士(理学)(東京工業大学)

    Research History

    • From Aug. 2020, To Present
      Kyoto University, Graduate School of Medicine, Program Specific Assistant Professor
    • From May 2019, To Jul. 2020
      Nagoya University, 未来材料・システム研究所付属 高度計測術実践センターナノ加工計測部, Researcher
    • From Apr. 2014, To Apr. 2019
      Nagoya University, School of Science, Structural Biology Research Center, Researcher
    • From Apr. 2013, To Mar. 2014
      Kyushu University, Institute for Materials Chemistry and Engineering, Research Assistant Professor
    • From Apr. 2012, To Mar. 2013
      Kyushu University, Institute for Materials Chemistry and Engineering, Post-doctoral Fellow

    Profile

    • Profile

      Now, I'm  researching the function of cytoskeleton, mainly actin, by biochemistry and microscope analysis at Kyoto university. Previously, I observed bio-molecules, for example F1-ATPase, and several materials including organic and inorganic molecules by using electoric microscope, high speed scanning atomic foce microscope(BIXAM, OLYMPUS), and optical microscope. 

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    list
      Last Updated :2022/06/30

      Research

      Research Interests

      • Molecular biology
      • single molecule observation
      • high speed scanning atomic force microscope
      • electronic microscope
      • morphology

      Research Areas

      • Life sciences, Biophysics, microscopy
      • Life sciences, Morphology, anatomy, Cytoskeleton

      Papers

      • A mechanism with severing near barbed ends andannealing explains structure and dynamics of dendriticactin networks
        Danielle Holz; Aaron R Hall; Eiji Usukura; Sawako Yamashiro; Naoki Watanabe; Dimitrios Vavylonis
        eLife, 07 Jun. 2022, Peer-reviewed
      • A cryo-TSEM with temperature cycling capability allows deep sublimation of ice to uncover fine structures in thick cells
        Jiro Usukura; Akihiro Narita; Tomoharu Matsumoto; Eiji Usukura; Takeshi Sunaoshi; Syunya Watanabe; Yusuke Tamba; Yasuhira Nagakubo; Takashi Mizuo; Junzo Azuma; Masako Osumi; Kazutaka Nimura; Ryuichiro Tamochi; Yoichi Ose
        Scientific Reports, Dec. 2021, Peer-reviewed
      • Improved unroofing protocols for cryo-electron microscopy, atomic force microscopy and freeze-etching electron microscopy and the associated mechanisms.
        Nobuhiro Morone; Eiji Usukura; Akihiro Narita; Jiro Usukura
        Microscopy (Oxford, England), 23 May 2020, Peer-reviewed
      • LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet
        Eiji Usukura; Yuhki Yanase; Ayumi Ishijima; Thasaneeya Kuboki; Satoru Kidoaki; Koichi Okamoto; Kaoru Tamada
        PLOS ONE, Dec. 2017, Peer-reviewed
      • A Cryosectioning Technique for the Observation of Intracellular Structures and Immunocytochemistry of Tissues in Atomic Force Microscopy (AFM)
        Eiji Usukura; Akihiro Narita; Akira Yagi; Nobuaki Sakai; Yoshitsugu Uekusa; Yuka Imaoka; Shuichi Ito; Jiro Usukura
        SCIENTIFIC REPORTS, Jul. 2017, Peer-reviewed
      • High-resolution imaging of a cellattached nanointerface using a gold-nanoparticle two-dimensional sheet
        Shihomi Masuda; Yuhki Yanase; Eiji Usukura; Sou Ryuzaki; Pangpang Wang; Koichi Okamoto; Thasaneeya Kuboki; Satoru Kidoaki; Kaoru Tamada
        SCIENTIFIC REPORTS, Jun. 2017, Peer-reviewed
      • A new approach for the direct visualization of the membrane cytoskeleton in cryo-electron microscopy: a comparative study with freeze-etching electron microscopy
        Masaki Makihara; Takashi Watanabe; Eiji Usukura; Kozo Kaibuchi; Akihiro Narita; Nobuo Tanaka; Jiro Usukura
        MICROSCOPY, Dec. 2016, Peer-reviewed
      • Direct observation of the actin filament by tip-scan atomic force microscopy
        Akihiro Narita; Eiji Usukura; Akira Yagi; Kiyohiko Tateyama; Shogo Akizuki; Mahito Kikumoto; Tomoharu Matsumoto; Yuichiro Maeda; Shuichi Ito; Jiro Usukura
        MICROSCOPY, Aug. 2016, Peer-reviewed
      • An Unroofing Method to Observe the Cytoskeleton Directly at Molecular Resolution Using Atomic Force Microscopy
        Eiji Usukura; Akihiro Narita; Akira Yagi; Shuichi Ito; Jiro Usukura
        SCIENTIFIC REPORTS, Jun. 2016, Peer-reviewed
      • Highly confined, enhanced surface fluorescence imaging with two-dimensional silver nanoparticle sheets
        Eiji Usukura; Shuhei Shinohara; Koichi Okamoto; Jaehoon Lim; Kookheon Char; Kaoru Tamada
        APPLIED PHYSICS LETTERS, Mar. 2014, Peer-reviewed
      • High-sensitivity surface plasmon resonance sensors utilizing high-refractive-index silver nanoparticle sheets
        Daisuke Tanaka; Shuhei Shinohara; Eiji Usukura; Pangpang Wang; Koichi Okamoto; Kaoru Tamada
        JAPANESE JOURNAL OF APPLIED PHYSICS, Jan. 2014, Peer-reviewed
      • Torque Generation and Utilization in Motor Enzyme F0F1-ATP Synthase HALF-TORQUE F-1 WITH SHORT-SIZED PUSHROD HELIX AND REDUCED ATP SYNTHESIS BY HALF-TORQUE F0F1
        Eiji Usukura; Toshiharu Suzuki; Shou Furuike; Naoki Soga; Ei-ichiro Saita; Toru Hisabori; Kazuhiko Kinosita; Masasuke Yoshida
        JOURNAL OF BIOLOGICAL CHEMISTRY, Jan. 2012, Peer-reviewed
      • Single molecule and single quantum dot photodynamics by polarization-rotating modulation microscopy
        Toshiro Tani; Hiroshi Sakai; Eiji Usukura; Tetsu Suzuki; Masaru Oda
        PROCEEDINGS OF THE TENTH INTERNATIONAL MEETING ON HOLE BURNING, SINGLE MOLECULE AND RELATED SPECTROSCOPIES, 2010, Peer-reviewed
      • Energy transfer in hybrid CdSe quantum dots vs. labelled molecular chaperone systems by imaging microscopy
        Toshiro Tani; Hiromi Horiuchi; Masaru Oda; Eiji Usukura; Hiroshi Sakai; Akashi Ohtaki; Masafumi Yohda
        PHYSICA STATUS SOLIDI C - CURRENT TOPICS IN SOLID STATE PHYSICS, VOL 6, NO 4, 2009, Peer-reviewed

      Misc.

      • LSPR-TIRF顕微鏡を用いた高分解能細胞イメージング
        増田志穂美; 柳瀬雄輝; 臼倉英治; 岡本晃一; 玉田薫
        応用物理学会春季学術講演会講演予稿集(CD-ROM), 2016
      • 金属微粒子二次元シートによるバイオインターフェイスの高分解能蛍光観察
        玉田薫; 臼倉英治; 増田志穂美; 田中大輔; 岡本晃一; 柳瀬雄輝
        応用物理学会春季学術講演会講演予稿集(CD-ROM), 2015
      • 金・銀ナノ粒子混合LB膜の局在プラズモン共鳴特性
        田中大輔; 臼倉英治; 大岩さゆり; 今津圭介; 岡本晃一; 玉田薫
        応用物理学会春季学術講演会講演予稿集(CD-ROM), 2014
      • Atomic Force Microscopy of the Cell
        Usukura E; Usukura J
        顕微鏡, Apr. 2020
      • 原子間力顕微鏡による細胞膜内面の構造解析を可能にしたアンルーフ法
        臼倉英治; 成田哲博; 臼倉治郎
        生物物理, 2019, Peer-reviewed, Invited
      • Cryo-electron microscope developed for simultaneous STEM, SEM imaging and its application to biological samples.
        Usukura J; Narita A; Matsumoto T; Usukura E; Sunaohi T; Tamba Y; Nagakubo Y; Azuma J; Mizuo T; Nimura K; Osumi M; Tamochi R; Ose Y
        Scientific Instrument News, 2018, Invited
      • Atomic force microscopic (AFM) imaging of muscle sarcomere at high resolution
        E. Usukura; A. Narita; A. Yagi; S. Itoh; J. Usukura
        MOLECULAR BIOLOGY OF THE CELL, 2016
      • Atomic force microscopic (AFM) imaging of cytoskeleton in retinal photoreceptor cells in cryosection.
        E. Usukura; A. Narita; A. Yagi; S. Ito; J. Usukura
        MOLECULAR BIOLOGY OF THE CELL, 2016
      • Unroofing methods in AFM imaging of intra-cellar cytoskeletons at molecular resolution
        E. Usukura; A. Narita; A. Yagi; S. Ito; J. Usukura
        MOLECULAR BIOLOGY OF THE CELL, 2015
      • Development of low voltage cryo-electron microscope for simultaneous imaging of bright field, dark field STEM and SEM using unroofed frozen cells
        J. Usukura; E. Usukura; T. Matsumoto; A. Narita
        MOLECULAR BIOLOGY OF THE CELL, 2015

      Presentations

      • Motion analysis of actin oligomer in lamellipodia by SiMS microscopy
        Eiji Usukura; Sawako Yamashiro; Naoki Watanabe
        2022年生体運動研究合同班会議, 08 Jan. 2022
      • AFM細胞生物学の最先端、live cell imagingから免疫細胞化学まで
        臼倉 英治
        第124回日本解剖学会, 28 Mar. 2019, Invited
      • Cutting Edge of Atomic Force Microscopy (AFM) of the Cell: Live Cell imaging and Structure Analysis of Cytoskeletal Actin Filaments at High Resolution
        Usukura Eiji
        19th International Microscopy Congress, Sep. 2018
      • Anew approach for the direct visualization of the membrane cytoskeleton in cryo-electron microscopy: a comparative study with freeze-etching electron microscopy
        Usukura Eiji
        2018年日本顕微鏡学会, May 2018, Invited
      • A Cryosectioning Technique for the Observation of Intracellular Structures and Immunocytochemistry of Tissues in Atomic Force Microscopy.
        Usukura Eiji
        2017 ASCB Annual Meeting, Dec. 2017
      • Unroofing技術を応用したAFMによる膜細胞骨格の高分解能解析
        臼倉 英治
        2017年生体運動研究合同会, 07 Jan. 2017
      • Atomic force microscopic (AFM) imaging of muscle sarcomere at high resolution
        Usukura Eiji
        2016 ASCB Annual Meeting, 06 Dec. 2016
      • An Unroofing Method to Observe the Cytoskeleton Directly at Molecular Resolution Using Atomic Force Microscopy
        Usukura Eiji
        2016年日本生物物理学会, Sep. 2016
      • Unroofing methods in AFM imaging for intra-cellar cytoskeletons at molecular resolution
        Usukura Eiji
        2015 ASCB Annual Meeting, 14 Dec. 2015
      • 銀微粒子2次元シート基板を利用した蛍光顕微鏡観察
        臼倉 英治
        第33回表面科学学術講演会, Nov. 2013
      • 銀微粒子2次元シート基板の蛍光増強効果
        臼倉 英治
        第64回コロイドおよび界面化学討論会, Sep. 2013
      • 銀ナノ粒子2次元シートの電場増強効果を利用した蛍光増強基板
        臼倉 英治
        第60回応用物理学会春季学術講演会, Mar. 2013
      • Fluorescence enhancement by 2D nanoparticle sheet
        Usukura Eiji
        Seventh International Conference on Molecular Electoronics and Bioelectoronics, Mar. 2013
      • Bacillus PS3 TF1の回転力発生機構の解明
        臼倉 英治
        第82回日本生化学会大会, Oct. 2009
      • Single molecule detection of the complexes between nanocrystal and molecular chaperoi
        Usukura Eiji
        日本物理学会第62回年次大会, Sep. 2007
      • 水溶液中のCdSe/ZnS/TOPO微結晶-分子シャペロン複合体の形成と顕微画像計測
        臼倉 英治
        第17回 光物性研究会, 08 Dec. 2006

      Books and Other Publications

      • Microscopy
        Eiji Usukura; Jiro Usukura, Joint work, Atomic Force Microscopy of the Cell
        The Japanese Society of Microscopy, May 2020
      • 生物物理誌Vol. 59 No. 4(通巻344号)
        臼倉 英治; 成田 哲博; 臼倉 治郎, Joint work, 原子間力顕微鏡による細胞膜内面の構造解析を可能にしたアンルーフ法
        日本生物物理学会, Aug. 2019, Not refereed

      External funds: Kakenhi

      • Dynamics analysis of virus entry and budding by high speed AFM
        Grant-in-Aid for Scientific Research (C)
        Basic Section 43040:Biophysics-related
        Kyoto University;Nagoya University
        臼倉 英治
        From 01 Apr. 2019, To 31 Mar. 2022, Granted
        インフルエンザウイルス;電子顕微鏡;高速走査型原子間力顕微鏡;エンドサイトーシス;1分子観察;原子間力顕微鏡;living cell imaging;ウイルス
      • Observation and analysis of muscle tissue by high-speed AFM
        Grant-in-Aid for Young Scientists (B)
        Nagoya University
        Eiji Usukura
        From 01 Apr. 2017, To 31 Mar. 2019, Project Closed
        高速AFM;徳安法;組織;細胞;蛍光顕微鏡;原子間力顕微鏡;組織観察;細胞・組織;走査プローブ顕微鏡;生物物理;病理学

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