Basic Information

Professional Memberships

• 日本細胞生物学会
• 米国生物物理学会
• 日本蛋白質科学会
• 日本生物物理学会

Academic Degree

• 修士（農学）(東京大学)
• 博士（農学）(東京大学)

Academic Resume (Graduate Schools)

• 東京大学, 大学院農学生命科学研究科修士課程応用生命工学専攻, 修了
• 東京大学, 大学院農学生命科学研究科博士課程応用生命工学専攻, 修了

Academic Resume (Undergraduate School/Majors)

• 東京大学, 農学部農芸化学科, 卒業
• 東京大学, 教養学部理科Ⅰ類

Language of Instruction

• English

ID,URL

• ORCID ID

  0000-0002-1896-0443

• J-Global ID

  200901024729974107

• Researcher number

  20422545

• External link

  http://www.imamura.lif.kyoto-u.ac.jp/index.html

Website(s) (URL(s))

• http://www.imamura.lif.kyoto-u.ac.jp/index.html

researchmap URL

• https://researchmap.jp/hiromi-imamura

Research

Research Topics, Overview of the research

• Research Topics

  Visualization of cellular energy metabolism

• Overview of the research

  タンパク質の働きを利用して、生物内部の情報、特にエネルギー物質の分布と動きを高い空間分解能と時間分解能で可視化するための技術を開発している。生体内のエネルギー物質を「観る」ことで、環境の変化に応答して生物が自らのエネルギー代謝状態を適応させるしくみの解明を目指す。

Research Interests

• 蛍光イメージング

Research Areas

• Life sciences, Biophysics
• Life sciences, Functional biochemistry
• Life sciences, Structural biochemistry

Papers

• Visualizing ATP Dynamics in Live Mice

  Norimichi Koitabashi; Riki Ogasawara; Ryuto Yasui; Yuki Sugiura; Hinako Matsuda; Shigenori Nonaka; Takashi Izumi; Masahiko Kurabayashi; Makoto Suematsu; Motoko Yanagita; Hiromi Imamura; Masamichi Yamamoto

  bioRxiv, 10 Jun. 2020

  
• 1P525 Designing a mutant F_1-ATPase for easy and rapid single molecule analysis(26. Single molecule biophysics,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

  Nakamura Mariko; Ueno Hiroshi; Imamura Hiromi; Noji Hiroyuki

  Seibutsu Butsuri, 2006

  
• 3P160 Elucidatoin of the role of arginine finger in F_1-ATPase(Molecular motors,Poster Presentations)

  Komoriya Yoshiihto; Okuno Daichi; Imamura Hiromi; Iino Ryota; noji Hiroyuki

  Seibutsu Butsuri, 2007

  
• 3P162 Determination the relationship between crystal structure and chemical state of F_1-ATPase by single molecule analysis(Molecular motors,Poster Presentations)

  Okuno Daichi; Hirono-Hara Yoko; Fujisawa Ryo; Imamura Hiromi; Iko Yuko; Noji Hiroyuki

  Seibutsu Butsuri, 2007

  
• 3P171 Pausing position of V _1-ATPase in the presence of ADP(Molecular motors,Poster Presentations)

  Nishikawa Yoshihiro; Imamura Hiromi; Yokoyama Ken; Noji Hiroyuki

  Seibutsu Butsuri, 2007

  
• 3P326 Fluorescent visualization of intracellular ATP(Bioimaging,Poster Presentations)

  Imamura Hiromi; Kim Huynh Nhat Phuong; Saito Kenta; Iino Ryota; Kato-Yamada Yasuyuki; Nagai Takeharu; Noji Hiroyuki

  Seibutsu Butsuri, 2007

  
• 3P037 N-terminal domain of F_1-ATPase ε subunit affects ATP binding to the C-terminal domain(Proteins-structure and structure-function relationship,Poster Presentations)

  Togawa Hiroko; Imamura Hiromi; Noji Hiroyuki

  Seibutsu Butsuri, 2007

  
• 1P188 Reconstitution of V_1 complex of Thermus thermophilus V-ATPase revealed that ATP binding to the A subunit is crucial for V_1 formation(6. Macromolecular assembly,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

  Imamura Hiromi; Funamoto Saeko; Yoshida Masasuke; Yokoyama Ken

  Seibutsu Butsuri, 2006

  
• 2P189 ATP-driven rotation of VoV1 from Thermus thermophilus(37. Molecular motor (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

  Nakano Masahiro; Tamakoshi Masatada; Imamura Hiromi; Yoshida Masasuke; Yokoyama Ken

  Seibutsu Butsuri, 2006

  
• 1P533 Mechanism of Inhibition of the V-type Molecular Motor by Tributyltin Chloride(26. Single molecule biophysics,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

  Takeda Mizuho; Imamura Hiromi; Shimabukuro Katsuya; Ikeda Chiyo; Yoshida Masasuke; Yokoyama Ken

  Seibutsu Butsuri, 2006

  
• 3P162 Development of new experimental system toward the visualization of the rotation of V-ATPase driven by the membrane potential

  Iino R.; Nakano M.; Tabata K.; Imamura H.; Tamakoshi M.; Yokoyama K.; Noji H.

  Seibutsu Butsuri, 2005

  
• 2P178 Rotation mechanism of V_1 is distinct from that of F_1

  Imamura H.; Takeda M.; Funamoto S.; Yoshida M.; Yokoyama K.

  Seibutsu Butsuri, 2005

  
• 1P154 Inhibitory effeet of TBT-Cl on V-ATPase activity

  Takeda M.; Imamura H.; Yoshida M.; Yokoyama K.

  Seibutsu Butsuri, 2005

  
• 1P155 Identification of stator subunit in V-ATPase

  Nakano M.; Tamakoshi M.; Imamura H.; Iino R.; Yoshida M.; Yokoyama K.

  Seibutsu Butsuri, 2005

  
• 3P139 Stepwise rotation of V_1ATPase

  Imamura H.; Funamoto S.; Yoshida M.; Yokoyama K.

  Seibutsu Butsuri, 2004

  
• 2N1412 Real-time observation of the ATP concentration inside E. coli cells using ATP biosensor "ATeam"(Bioimaging 2,The 48th Annual Meeting of the Biophysical Society of Japan)

  Yaginuma Hideyuki; Tomiyama Keisuke; Tabata Kazuhito; Imamura Hiromi; Noji Hiroyuki

  Seibutsu Butsuri, 2011

  
• 1P326 1J1240 Red-shifted genetically-encoded fluorescent ATP indicator enabled simultaneous imaging of ATP and calcium ion inside single living cells(Bioimaging,Oral Presentations,The 48th Annual Meeting of the Biophysical Society of Japan)

  Nakano Masahiro; Imamura Hiromi; Noji Hiroyuki

  Seibutsu Butsuri, 2010

  
• 1P-259 Single cell imaging of ATP level inside bacteria(Bioimaging, The 47th Annual Meeting of the Biophysical Society of Japan)

  Tomiyama Keisuke; Nakano Masahiro; Imamura Hiromi; Noji Hiroyuki

  Seibutsu Butsuri, 2009

  
• 3P-251 Imaging of intracellular ATP dynamics during cell division cycle(Bioimaging,The 47th Annual Meeting of the Biophysical Society of Japan)

  Imamura Hiromi; Noji Hiroyuki

  Seibutsu Butsuri, 2009

  
• 3P-131 Distinctive rotational steps of V-ATPase in ATP-driven rotation(The 46th Annual Meeting of the Biophysical Society of Japan)

  Furuike Shou; Nakano Masahiro; Imamura Hiromi; Adachi Kengo; Noji Hiroyuki; Yoshida Masasuke; Kinoshita Jr. Kazuhiko; Yokoyama Ken

  Seibutsu Butsuri, 2008

  
• 3P-148 Clarification of the function of arginine Arg-finger in F_1-ATPase(The 46th Annual Meeting of the Biophysical Society of Japan)

  Komoriya Yoshihito; Iino Ryota; Okuno Daichi; Imamura Hiromi; Ariga Tkayuki; Noji Hiroyuki

  Seibutsu Butsuri, 2008

  
• 3P-129 Analysis of inactive V_1-ATPase during rotation by single molecule measurement(The 46th Annual Meeting of the Biophysical Society of Japan)

  Nishikawa Yoshihiro; Okuno Daichi; Nakano Masahiro; Imamura Hiromi; Yokoyama Ken; Noji Hiroyuki

  Seibutsu Butsuri, 2008

  
• 3P-325 Spatio-temporal dynamics of intracellular ATP during apoptosis revealed by a genetically encoded fluorescent ATP indicator(The 46th Annual Meeting of the Biophysical Society of Japan)

  Imamura Hiromi; Saito Kenta; Iino Ryota; Yamada Yasuyuki; Nagai Takeharu; Noji Hiroyuki

  Seibutsu Butsuri, 2008

  
• High and stable ATP levels prevent aberrant intracellular protein aggregation in yeast.

  Masak Takaine; Hiromi Imamura; Satoshi Yoshida

  eLife, 19 Apr. 2022

  
• Lactate is an energy substrate for rodent cortical neurons and enhances their firing activity.

  Anastassios Karagiannis; Thierry Gallopin; Alexandre Lacroix; Fabrice Plaisier; Juliette Piquet; Hélène Geoffroy; Régine Hepp; Jérémie Naudé; Benjamin Le Gac; Richard Egger; Bertrand Lambolez; Dongdong Li; Jean Rossier; Jochen F Staiger; Hiromi Imamura; Susumu Seino; Jochen Roeper; Bruno Cauli

  eLife, 12 Nov. 2021

  
• Shear stress activates mitochondrial oxidative phosphorylation by reducing plasma membrane cholesterol in vascular endothelial cells.

  Kimiko Yamamoto; Yoshitsugu Nogimori; Hiromi Imamura; Joji Ando

  Proceedings of the National Academy of Sciences of the United States of America, 29 Dec. 2020

  
• Spatiotemporal ATP Dynamics during AKI Predict Renal Prognosis.

  Shinya Yamamoto; Masamichi Yamamoto; Jin Nakamura; Akiko Mii; Shigenori Yamamoto; Masahiro Takahashi; Keiichi Kaneko; Eiichiro Uchino; Yuki Sato; Shingo Fukuma; Hiromi Imamura; Michiyuki Matsuda; Motoko Yanagita

  Journal of the American Society of Nephrology : JASN, Dec. 2020

  
• Single-cell dynamics of pannexin-1-facilitated programmed ATP loss during apoptosis.

  Hiromi Imamura; Shuichiro Sakamoto; Tomoki Yoshida; Yusuke Matsui; Silvia Penuela; Dale W Laird; Shin Mizukami; Kazuya Kikuchi; Akira Kakizuka

  eLife, 14 Oct. 2020

  
• Intracellular ATP levels in mouse cortical excitatory neurons varies with sleep-wake states.

  Akiyo Natsubori; Tomomi Tsunematsu; Akihiro Karashima; Hiromi Imamura; Naoya Kabe; Andrea Trevisiol; Johannes Hirrlinger; Tohru Kodama; Tomomi Sanagi; Kazuto Masamoto; Norio Takata; Klaus-Armin Nave; Ko Matsui; Kenji F Tanaka; Makoto Honda

  Communications biology, 07 Sep. 2020

  
• In vivo real-time ATP imaging in zebrafish hearts reveals G0s2 induces ischemic tolerance.

  Hidetaka Kioka; Hisakazu Kato; Takeshi Fujita; Yoshihiro Asano; Yasunori Shintani; Satoru Yamazaki; Osamu Tsukamoto; Hiromi Imamura; Mikihiko Kogo; Masafumi Kitakaze; Yasushi Sakata; Seiji Takashima

  FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Feb. 2020, Peer-reviewed

  
• Monitoring and mathematical modeling of mitochondrial ATP in myotubes at single-cell level reveals two distinct population with different kinetics.

  Naoki Matsuda; Ken-ichi Hironaka; Masashi Fujii; Takumi Wada; Katsuyuki Kunida; Haruki Inoue; Miki Eto; Daisuke Hoshino; Yasuro Furuichi; Yasuko Manabe; Nobuharu L. Fujii; Hiroyuki Noji; Hiromi Imamura; Shinya Kuroda

  Quant. Biol., 2020

  
• Measurement of ATP concentrations in mitochondria of living cells using luminescence and fluorescence approaches.

  Giampaolo Morciano; Hiromi Imamura; Simone Patergnani; Gaia Pedriali; Carlotta Giorgi; Paolo Pinton

  Methods in cell biology, 2020

  
• Higd1a improves respiratory function in the models of mitochondrial disorder.

  Takemasa Nagao; Yasunori Shintani; Takaharu Hayashi; Hidetaka Kioka; Hisakazu Kato; Yuya Nishida; Satoru Yamazaki; Osamu Tsukamoto; Shohei Yashirogi; Issei Yazawa; Yoshihiro Asano; Kyoko Shinzawa-Itoh; Hiromi Imamura; Takeo Suzuki; Tsutomu Suzuki; Yu-Ichi Goto; Seiji Takashima

  FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Jan. 2020, Peer-reviewed

  
• OLIVe: A Genetically Encoded Fluorescent Biosensor for Quantitative Imaging of Branched-Chain Amino Acid Levels inside Single Living Cells.

  Tomoki Yoshida; Hitomi Nakajima; Sena Takahashi; Akira Kakizuka; Hiromi Imamura

  ACS sensors, 27 Dec. 2019, Peer-reviewed

  
• PPARα-Mediated Positive-Feedback Loop Contributes to Cold Exposure Memory

  Soaad Alfaqaan; Tomoki Yoshida; Hiromi Imamura; Chihiro Tsukano; Yoshiji Takemoto; Akira Kakizuka

  Scientific Reports, 01 Dec. 2019, Peer-reviewed

  
• A molecular triage process mediated by RING finger protein 126 and BCL2-associated athanogene 6 regulates degradation of G0/G1 switch gene 2.

  Kenta Kamikubo; Hisakazu Kato; Hidetaka Kioka; Satoru Yamazaki; Osamu Tsukamoto; Yuya Nishida; Yoshihiro Asano; Hiromi Imamura; Hiroyuki Kawahara; Yasunori Shintani; Seiji Takashima

  The Journal of biological chemistry, 04 Oct. 2019, Peer-reviewed

  
• NAD+ consumption by PARP1 in response to DNA damage triggers metabolic shift critical for damaged cell survival.

  Michael M Murata; Xiangduo Kong; Emmanuel Moncada; Yumay Chen; Hiromi Imamura; Ping Wang; Michael W Berns; Kyoko Yokomori; Michelle A Digman

  Molecular biology of the cell, 15 Sep. 2019, Peer-reviewed

  
• Reliable imaging of ATP in living budding and fission yeast

  Masak Takaine; Masaru Ueno; Kenji Kitamura; Hiromi Imamura; Satoshi Yoshida

  Journal of Cell Science, 15 Apr. 2019, Peer-reviewed

  
• Spindle pole body movement is affected by glucose and ammonium chloride in fission yeast

  Hiroaki Ito; Takeshi Sugawara; Soya Shinkai; Satoshi Mizukawa; Ayaka Kondo; Hisamichi Senda; Kengo Sawai; Koki Ito; Sayaka Suzuki; Masakatsu Takaine; Satoshi Yoshida; Hiromi Imamura; Kenji Kitamura; Toshinori Namba; Shin-ichi Tate; Masaru Ueno

  Biochemical and Biophysical Research Communications, Apr. 2019, Peer-reviewed

  
• A Trace Amount of Galactose, a Major Component of Milk Sugar, Allows Maturation of Glycoproteins during Sugar Starvation

  Norio Sasaoka; Hiromi Imamura; Akira Kakizuka

  iScience, Dec. 2018, Peer-reviewed

  
• Automatic Quantitative Segmentation of Myotubes Reveals Single-cell Dynamics of S6 Kinase Activation.

  Inoue H; Kunida K; Matsuda N; Hoshino D; Wada T; Imamura H; Noji H; Kuroda S

  Cell structure and function, Aug. 2018, Peer-reviewed

  
• Shear stress augments mitochondrial ATP generation that triggers ATP release and Ca²⁺ signaling in vascular endothelial cells.

  Yamamoto K; Imamura H; Ando J

  American journal of physiology. Heart and circulatory physiology, Aug. 2018, Peer-reviewed

  
• Human AK2 links intracellular bioenergetic redistribution to the fate of hematopoietic progenitors.

  Koichi Oshima; Norikazu Saiki; Michihiro Tanaka; Hiromi Imamura; Akira Niwa; Ayako Tanimura; Ayako Nagahashi; Akiyoshi Hirayama; Keisuke Okita; Akitsu Hotta; Shuichi Kitayama; Mitsujiro Osawa; Shin Kaneko; Akira Watanabe; Isao Asaka; Wataru Fujibuchi; Kohsuke Imai; Hiromasa Yabe; Yoshiro Kamachi; Junichi Hara; Seiji Kojima; Masaru Tomita; Tomoyoshi Soga; Takafumi Noma; Shigeaki Nonoyama; Tatsutoshi Nakahata; Megumu K Saito

  Biochemical and biophysical research communications, 04 Mar. 2018, Peer-reviewed

  
• A Transient Rise in Free Mg2+ Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation

  Kazuhiro Maeshima; Tomoki Matsuda; Yutaka Shindo; Hiromi Imamura; Sachiko Tamura; Ryosuke Imai; Syoji Kawakami; Ryosuke Nagashima; Tomoyoshi Soga; Hiroyuki Noji; Kotaro Oka; Takeharu Nagai

  Current Biology, 05 Feb. 2018, Peer-reviewed

  
• Analysis of mitochondrial function in human induced pluripotent stem cells from patients with mitochondrial diabetes due to the A3243G mutation.

  Masaki Matsubara; Hajime Kanda; Hiromi Imamura; Mayumi Inoue; Michio Noguchi; Kiminori Hosoda; Akira Kakizuka; Kazuwa Nakao

  Scientific reports, 17 Jan. 2018, Peer-reviewed

  
• General anesthetics cause mitochondrial dysfunction and reduction of intracellular ATP levels

  Jun-ichi Kishikawa; Yuki Inoue; Makoto Fujikawa; Kenji Nishimura; Atsuko Nakanishi; Tsutomu Tanabe; Hiromi Imamura; Ken Yokoyama

  PLoS ONE, 01 Jan. 2018, Peer-reviewed

  
• Visualization of long-term Mg2+ dynamics in apoptotic cells using a novel targetable fluorescent probe

  Yusuke Matsui; Yosuke Funato; Hiromi Imamura; Hiroaki Miki; Shin Mizukami; Kazuya Kikuchi

  CHEMICAL SCIENCE, Dec. 2017, Peer-reviewed

  
• Fusion protein analysis reveals the precise regulation between Hsp70 and Hsp100 during protein disaggregation

  Sayaka Hayashi; Yosuke Nakazaki; Kei Kagii; Hiromi Imamura; Yo-hei Watanabe

  SCIENTIFIC REPORTS, Aug. 2017, Peer-reviewed

  
• ATP Maintenance via Two Types of ATP Regulators Mitigates Pathological Phenotypes in Mouse Models of Parkinson's Disease

  Masaki Nakano; Hiromi Imamura; Norio Sasaoka; Masamichi Yamamoto; Norihito Uemura; Toshiyuki Shudo; Tomohiro Fuchigami; Ryosuke Takahashi; Akira Kakizuka

  EBIOMEDICINE, Aug. 2017, Peer-reviewed

  
• RLR-mediated antiviral innate immunity requires oxidative phosphorylation activity

  Takuma Yoshizumi; Hiromi Imamura; Tomohiro Taku; Takahiro Kuroki; Atsushi Kawaguchi; Kaori Ishikawa; Kazuto Nakada; Takumi Koshiba

  SCIENTIFIC REPORTS, Jul. 2017, Peer-reviewed

  
• タンパク質ラベル化型Mg2+プローブによるアポトーシス時の細胞内Mg2+動態イメージング

  松井 勇輔; 水上 進; 船戸 洋佑; 今村 博臣; 三木 裕明; 菊地 和也

  日本細胞生物学会大会講演要旨集, May 2017

  
• Cell competition with normal epithelial cells promotes apical extrusion of transformed cells through metabolic changes.

  Shunsuke Kon; Kojiro Ishibashi; Hiroto Katoh; Sho Kitamoto; Takanobu Shirai; Shinya Tanaka; Mihoko Kajita; Susumu Ishikawa; Hajime Yamauchi; Yuta Yako; Tomoko Kamasaki; Tomohiro Matsumoto; Hirotaka Watanabe; Riku Egami; Ayana Sasaki; Atsuko Nishikawa; Ikumi Kameda; Takeshi Maruyama; Rika Narumi; Tomoko Morita; Yoshiteru Sasaki; Ryosuke Enoki; Sato Honma; Hiromi Imamura; Masanobu Oshima; Tomoyoshi Soga; Jun-Ichi Miyazaki; Michael R Duchen; Jin-Min Nam; Yasuhito Onodera; Shingo Yoshioka; Junichi Kikuta; Masaru Ishii; Masamichi Imajo; Eisuke Nishida; Yoichiro Fujioka; Yusuke Ohba; Toshiro Sato; Yasuyuki Fujita

  Nature cell biology, May 2017, Peer-reviewed

  
• Monitoring ATP dynamics in electrically active white matter tracts

  Andrea Trevisiol; Aiman S. Saab; Ulrike Winkler; Grit Marx; Hiromi Imamura; Wiebke Moebius; Kathrin Kusch; Klaus-Armin Nave; Johannes Hirrlinger

  ELIFE, Apr. 2017, Peer-reviewed

  
• Distinct intracellular Ca2+ dynamics regulate apical constriction and differentially contribute to neural tube closure

  Makoto Suzuki; Masanao Sato; Hiroshi Koyama; Yusuke Hara; Kentaro Hayashi; Naoko Yasue; Hiromi Imamura; Toshihiko Fujimori; Takeharu Nagai; Robert E. Campbell; Naoto Ueno

  DEVELOPMENT, Apr. 2017, Peer-reviewed

  
• Mitochondrial dysfunction induces dendritic loss via eIF2 alpha phosphorylation

  Taiichi Tsuyama; Asako Tsubouchi; Tadao Usui; Hiromi Imamura; Tadashi Uemura

  JOURNAL OF CELL BIOLOGY, Mar. 2017, Peer-reviewed

  
• Evaluation of Mitochondrial Respiratory Activity Using a FRET-based Indicator for Intracellular ATP

  KOSHIBA Takumi; IMAMURA Hiromi

  Seibutsu Butsuri, 2017

  
• Application of FRET-based biosensor “ATeam” for visualization of ATP levels in the mitochondrial matrix of living mammalian cells

  Tomoki Yoshida; Soaad Alfaqaan; Norio Sasaoka; Hiromi Imamura

  Methods in Molecular Biology, 2017, Peer-reviewed

  
• BTeam, a Novel BRET-based Biosensor for the Accurate Quantification of ATP Concentration within Living Cells

  Tomoki Yoshida; Akira Kakizuka; Hiromi Imamura

  SCIENTIFIC REPORTS, Dec. 2016, Peer-reviewed

  
• 蛍光ATPプローブ遺伝子ノックインマウスを用いた膵β細胞内代謝解析

  杉崎 和; 山本 正道; 龍岡 久登; 臼井 亮太; 今村 博臣; 田原 裕美子; 小倉 かさね; 佐藤 広規; 佐藤 雄一; 小倉 雅仁; 長嶋 一昭; 稲垣 暢也

  糖尿病, Apr. 2016, Peer-reviewed

  
• Visualization of NO3-/NO2- Dynamics in Living Cells by Fluorescence Resonance Energy Transfer ( FRET) Imaging Employing a Rhizobial Two-component Regulatory System

  Masafumi Hidaka; Aina Gotoh; Taiki Shimizu; Kiwamu Minamisawa; Hiromi Imamura; Takafumi Uchida

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jan. 2016, Peer-reviewed

  
• 腎臓糸球体構成細胞ポドサイト細胞における、ATP産生に注目した解糖系とミトコンドリアの異なる役割

  小沢 将太; 上田 修子; 今村 博臣; 森 潔; 淺沼 克彦; 柳田 素子; 仲川 孝彦

  日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, Dec. 2015

  
• Mild Glucose Starvation Induces KDM2A-Mediated H3K36me2 Demethylation through AMPK To Reduce rRNA Transcription and Cell Proliferation

  Yuji Tanaka; Hirohisa Yano; Sachiko Ogasawara; Sho-ichi Yoshioka; Hiromi Imamura; Kengo Okamoto; Makoto Tsuneoka

  MOLECULAR AND CELLULAR BIOLOGY, Dec. 2015, Peer-reviewed

  
• Glycolysis, but not Mitochondria, responsible for intracellular ATP distribution in cortical area of podocytes

  Shota Ozawa; Shuko Ueda; Hiromi Imamura; Kiyoshi Mori; Katsuhiko Asanuma; Motoko Yanagita; Takahiko Nakagawa

  SCIENTIFIC REPORTS, Dec. 2015, Peer-reviewed

  
• The Plasma Membrane Calcium Pump in Pancreatic Cancer Cells Exhibiting the Warburg Effect Relies on Glycolytic ATP

  Andrew D. James; Waseema Patel; Zohra Butt; Magretta Adiamah; Raga Dakhel; Ayse Latif; Carolina Uggenti; Eileithyia Swanton; Hiromi Imamura; Ajith K. Siriwardena; Jason I. E. Bruce

  JOURNAL OF BIOLOGICAL CHEMISTRY, Oct. 2015, Peer-reviewed

  
• Associative Interactions in Crowded Solutions of Biopolymers Counteract Depletion Effects

  Joost Groen; David Foschepoth; Esra te Brinke; Arnold J. Boersma; Hiromi Imamura; German Rivas; Hans A. Heus; Wilhelm T. S. Huck

  JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Oct. 2015, Peer-reviewed

  
• Mitochondrial dysfunction in primary human fibroblasts triggers an adaptive cell survival program that requires AMPK-alpha

  Felix Distelmaier; Federica Valsecchi; Dania C. Liemburg-Apers; Magdalena Lebiedzinska; Richard J. Rodenburg; Sandra Heil; Jaap Keijer; Jack Fransen; Hiromi Imamura; Katharina Danhauser; Annette Seibt; Benoit Viollet; Frank N. Gellerich; Jan A. M. Smeitink; Mariusz R. Wieckowski; Peter H. G. M. Willems; Werner J. H. Koopman

  BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, Mar. 2015, Peer-reviewed

  
• Mitochondrial dysfunction in primary human fibroblasts triggers an adaptive cell survival program that requires AMPK-α.

  Distelmaier F; Valsecchi F; Liemburg-Apers DC; Lebiedzinska M; Rodenburg RJ; Heil S; Keijer J; Fransen J; Imamura H; Danhauser K; Seibt A; Viollet B; Gellerich FN; Smeitink JA; Wieckowski MR; Willems PH; Koopman WJ

  Biochimica et biophysica acta, Mar. 2015, Peer-reviewed

  
• 根粒菌の二成分制御システムを利用したNO3-/NO2-バイオセンサーの開発

  後藤 愛那; 日高 將文; 清水 泰希; 板倉 学; クリスチーナ・サンチェス; 南澤 究; 今村 博臣; 内田 隆史

  日本生化学会大会プログラム・講演要旨集, Oct. 2014

  
• Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imaging

  Hideyuki Yaginuma; Shinnosuke Kawai; Kazuhito V. Tabata; Keisuke Tomiyama; Akira Kakizuka; Tamiki Komatsuzaki; Hiroyuki Noji; Hiromi Imamura

  SCIENTIFIC REPORTS, Oct. 2014, Peer-reviewed

  
• Toll-like receptor 9 protects non-immune cells from stress by modulating mitochondrial ATP synthesis through the inhibition of SERCA2

  Yasunori Shintani; Hannes C. A. Drexler; Hidetaka Kioka; Cesare M. N. Terracciano; Steven R. Coppen; Hiromi Imamura; Masaharu Akao; Junichi Nakai; Ann P. Wheeler; Shuichiro Higo; Hiroyuki Nakayama; Seiji Takashima; Kenta Yashiro; Ken Suzuki

  EMBO REPORTS, Apr. 2014, Peer-reviewed

  
• ATP increases within the lumen of the endoplasmic reticulum upon intracellular Ca2+ release

  Neelanjan Vishnu; Muhammad Jadoon Khan; Felix Karsten; Lukas N. Groschner; Markus Waldeck-Weiermair; Rene Rost; Seth Hallstroem; Hiromi Imamura; Wolfgang F. Graier; Roland Malli

  MOLECULAR BIOLOGY OF THE CELL, Feb. 2014, Peer-reviewed

  
• Glucose-stimulated Single Pancreatic Islets Sustain Increased Cytosolic ATP Levels during Initial Ca2+ Influx and Subsequent Ca2+ Oscillations

  Takashi Tanaka; Kazuaki Nagashima; Nobuya Inagaki; Hidetaka Kioka; Seiji Takashima; Hajime Fukuoka; Hiroyuki Noji; Akira Kakizuka; Hiromi Imamura

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jan. 2014, Peer-reviewed

  
• Evaluation of intramitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation

  Hidetaka Kioka; Hisakazu Kato; Makoto Fujikawa; Osamu Tsukamoto; Toshiharu Suzuki; Hiromi Imamura; Atsushi Nakano; Shuichiro Higo; Satoru Yamazaki; Takashi Matsuzaki; Kazuaki Takafuji; Hiroshi Asanuma; Masanori Asakura; Tetsuo Minamino; Yasunori Shintani; Masasuke Yoshida; Hiroyuki Noji; Masafumi Kitakaze; Issei Komuro; Yoshihiro Asano; Seiji Takashima

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Jan. 2014, Peer-reviewed

  
• Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells

  Shigeki Kiyonaka; Taketoshi Kajimoto; Reiko Sakaguchi; Daisuke Shinmi; Mariko Omatsu-Kanbe; Hiroshi Matsuura; Hiromi Imamura; Takenao Yoshizaki; Itaru Hamachi; Takashi Morii; Yasuo Mori

  NATURE METHODS, Dec. 2013, Peer-reviewed

  
• In Vivo Fluorescent Adenosine 5'-Triphosphate (ATP) Imaging of Drosophila melanogaster and Caenorhabditis elegans by Using a Genetically Encoded Fluorescent ATP Biosensor Optimized for Low Temperatures

  Taiichi Tsuyama; Jun-ichi Kishikawa; Yong-Woon Han; Yoshie Harada; Asako Tsubouchi; Hiroyuki Noji; Akira Kakizuka; Ken Yokoyama; Tadashi Uemura; Hiromi Imamura

  ANALYTICAL CHEMISTRY, Aug. 2013, Peer-reviewed

  
• Role of PFKFB3-Driven Glycolysis in Vessel Sprouting

  Katrien De Bock; Maria Georgiadou; Sandra Schoors; Anna Kuchnio; Brian W. Wong; Anna Rita Cantelmo; Annelies Quaegebeur; Bart Ghesquiere; Sandra Cauwenberghs; Guy Eelen; Li-Kun Phng; Inge Betz; Bieke Tembuyser; Katleen Brepoels; Jonathan Welti; Ilse Geudens; Inmaculada Segura; Bert Cruys; Franscesco Bifari; Ilaria Decimo; Raquel Blanco; Sabine Wyns; Jeroen Vangindertael; Susana Rocha; Russel T. Collins; Sebastian Munck; Dirk Daelemans; Hiromi Imamura; Roland Devlieger; Mark Rider; Paul P. Van Veldhoven; Frans Schuit; Ramon Bartrons; Johan Hofkens; Peter Fraisl; Sucheta Telang; Ralph J. DeBerardinis; Luc Schoonjans; Stefan Vinckier; Jason Chesney; Holger Gerhardt; Mieke Dewerchin; Peter Carmeliet

  CELL, Aug. 2013, Peer-reviewed

  
• Distribution and Dynamics of Intracellular ATP Level and their Regulation

  IMAMURA Hiromi; ANDO Tomomi; FUJIKAWA Makoto

  Seibutsu Butsuri, 25 Jan. 2013

  
• 3P133 ATP quantification and live-imaging in Xenopus laevis oocyte(09. Development & Differentation,Poster)

  Ijiri Takashi W.; Kishikawa Jun-ichi; Imamura Hiromi; Sakiie Maho; Ueno Shuichi; Iwao Yasuhiro; Yokoyama Ken; Sato Ken-ichi

  Seibutsu Butsuri, 2013

  
• 3P291 Quantifying the absolute ATP concentration inside single bacteria cells(27. Bioimaging,Poster)

  Yaginuma Hideyuki; Kawai Shinnosuke; Tomiyama Keisuke; Tabata Kazuhito V.; Komatsuzaki Tamiki; Imamura Hiromi; Noji Hiroyuki

  Seibutsu Butsuri, 2013

  
• Changes in Cytosolic ATP Levels and Intracellular Morphology during Bacteria-Induced Hypersensitive Cell Death as Revealed by Real-Time Fluorescence Microscopy Imaging

  Noriyuki Hatsugai; Vadim Perez Koldenkova; Hiromi Imamura; Hiroyuki Noji; Takeharu Nagai

  PLANT AND CELL PHYSIOLOGY, Oct. 2012, Peer-reviewed

  
• Spatiotemporal Correlations between Cytosolic and Mitochondrial Ca2+ Signals Using a Novel Red-Shifted Mitochondrial Targeted Cameleon

  Markus Waldeck-Weiermair; Muhammad Rizwan Alam; Muhammad Jadoon Khan; Andras T. Deak; Neelanjan Vishnu; Felix Karsten; Hiromi Imamura; Wolfgang F. Graier; Roland Malli

  PLOS ONE, Sep. 2012, Peer-reviewed

  
• Assessing Actual Contribution of IF1, Inhibitor of Mitochondrial FoF1, to ATP Homeostasis, Cell Growth, Mitochondrial Morphology, and Cell Viability

  Makoto Fujikawa; Hiromi Imamura; Junji Nakamura; Masasuke Yoshida

  JOURNAL OF BIOLOGICAL CHEMISTRY, May 2012, Peer-reviewed

  
• Principal Role of the Arginine Finger in Rotary Catalysis of F-1-ATPase

  Yoshihito Komoriya; Takayuki Ariga; Ryota Iino; Hiromi Imamura; Daichi Okuno; Hiroyuki Noji

  JOURNAL OF BIOLOGICAL CHEMISTRY, Apr. 2012, Peer-reviewed

  
• Visualization and Measurement of ATP Levels in Living Cells Replicating Hepatitis C Virus Genome RNA

  Tomomi Ando; Hiromi Imamura; Ryosuke Suzuki; Hideki Aizaki; Toshiki Watanabe; Takaji Wakita; Tetsuro Suzuki

  PLOS PATHOGENS, Mar. 2012, Peer-reviewed

  
• MRT letter: Expression of ATP sensor protein in Caenorhabditis elegans

  Jun-Ichi Kishikawa; Makoto Fujikawa; Hiromi Imamura; Kayo Yasuda; Hiroyuki Noji; Naoaki Ishii; Shohei Mitani; Ken Yokoyama

  MICROSCOPY RESEARCH AND TECHNIQUE, Jan. 2012, Peer-reviewed

  
• Quantitative Glucose and ATP Sensing in Mammalian Cells

  Dania C. Liemburg-Apers; Hiromi Imamura; Marleen Forkink; Marco Nooteboom; Herman G. Swarts; Roland Brock; Jan A. M. Smeitink; Peter H. G. M. Willems; Werner J. H. Koopman

  PHARMACEUTICAL RESEARCH, Nov. 2011, Peer-reviewed

  
• Leucine Zipper EF Hand-containing Transmembrane Protein 1 (Letm1) and Uncoupling Proteins 2 and 3 (UCP2/3) Contribute to Two Distinct Mitochondrial Ca2+ Uptake Pathways

  Markus Waldeck-Weiermair; Claire Jean-Quartier; Rene Rost; Muhammad Jadoon Khan; Neelanjan Vishnu; Alexander I. Bondarenko; Hiromi Imamura; Roland Malli; Wolfgang F. Graier

  JOURNAL OF BIOLOGICAL CHEMISTRY, Aug. 2011, Peer-reviewed

  
• Ca2+ Regulation of Mitochondrial ATP Synthesis Visualized at the Single Cell Level

  Masahiro Nakano; Hiromi Imamura; Takeharu Nagai; Hiroyuki Noji

  ACS CHEMICAL BIOLOGY, Jul. 2011, Peer-reviewed

  
• ATP concentration change in Caenorhabditis elegans

  Jun-ichi Kishikawa; Makoto Fujikawa; Hiromi Imamura; Kayo Yasuda; Naoaki Ishii; Shohei Mitani; Hiroyuki Noji; Ken Yokoyama

  BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Jul. 2010, Peer-reviewed

  
• Reversible Dimerization of Aequorea victoria Fluorescent Proteins Increases the Dynamic Range of FRET-Based Indicators

  Ippei Kotera; Takuya Iwasaki; Hiromi Imamura; Hiroyuki Noji; Takeharu Nagai

  ACS CHEMICAL BIOLOGY, Feb. 2010, Peer-reviewed

  
• Fluorescence imaging of intra- and extra-cellular ATP

  Hiromi Imamura

  Seikagaku, 2010, Peer-reviewed

  
• 蛍光イメージングによる細胞内および細胞外ATPの可視化

  今村博臣

  生化学, 2010

  
• 新規FRETプローブを用いた生細胞内ATP濃度イメージング法

  今村博臣

  実験医学, 2010

  
• [Imaging of intracellular ATP using novel fluorescent probes].

  Imamura H; Noji H; Tanpakushitsu kakusan; koso. Protein; nucleic acid; enzyme, vo

  Protein, nucleic acid and enzyme, Dec. 2009, Peer-reviewed

  
• Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators

  Hiromi Imamura; Kim P. Huynh Nhat; Hiroko Togawa; Kenta Saito; Ryota Iino; Yasuyuki Kato-Yamada; Takeharu Nagai; Hiroyuki Noji

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Sep. 2009, Peer-reviewed

  
• 新規蛍光プローブを用いた細胞内ATPイメージング

  今村博臣; 野地博行

  蛋白質・核酸・酵素, 2009

  
• Correlation between the conformational states of F-1-ATPase as determined from its crystal structure and single-molecule rotation

  Daichi Okuno; Ryo Fujisawa; Ryota Iino; Yoko Hirono-Hara; Hiromi Imamura; Hiroyuki Noji

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Dec. 2008, Peer-reviewed

  
• ATP hydrolysis and synthesis of a rotary motor V-ATPase from Thermus thermophilus

  Masahiro Nakano; Hiromi Imamura; Masashi Toei; Masatada Tamakoshi; Masasuke Yoshida; Ken Yokoyama

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jul. 2008, Peer-reviewed

  
• Two rotary molecular motors, V-ATPase and F0F1

  Hiromi Imamura; Ken Yokoyama

  SEIKAGAKU, May 2007, Peer-reviewed

  
• 二つの回転分子モータータンパク質；V-ATPaseとFoF1の共通性と多様性

  今村博臣; 横山謙

  生化学, 2007

  
• New Structural Insights on Carbohydrate-active Enzymes

  FUSHINOBU Shinya; HIDAKA Masafumi; MIYANAGA Akimasa; IMAMURA Hiromi

  Journal of Applied Glycoscience, 2007

  
• Reconstitution in vitro of V-1 complex of Thermus thermophilus V-ATPase revealed that ATP binding to the A subunit is crucial for V-1 formation

  Hiromi Imamura; Saeko Funamoto; Masasuke Yoshida; Ken Yokoyama

  JOURNAL OF BIOLOGICAL CHEMISTRY, Dec. 2006, Peer-reviewed

  
• Crystallization and preliminary X-ray analysis of cytosolic alpha-mannosidase from Thermotoga maritima

  M Nakajima; S Fushinobu; H Imamura; H Shoun; T Wakagi

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Feb. 2006, Peer-reviewed

  
• Rotation scheme of V-1-motor is different from that of F-1-motor

  H Imamura; M Takeda; S Funamoto; K Shimabukuro; M Yoshida; K Yokoyama

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Dec. 2005, Peer-reviewed

  
• Rotation, structure, and classification of prokaryotic V-ATPase

  K Yokoyama; H Imamura

  JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, Dec. 2005, Peer-reviewed

  
• Structure of a central stalk subunit F of prokaryotic V-type ATPase/synthase from Thermus thermophilus

  H Makyio; R Iino; C Ikeda; H Imamura; M Tamakoshi; M Iwata; D Stock; RA Bernal; EP Carpenter; M Yoshida; K Yokoyama; S Iwata

  EMBO JOURNAL, Nov. 2005, Peer-reviewed

  
• Transglycosylation activity of Dictyoglomus thermophilum amylase A

  M Nakajima; H Imamura; H Shoun; S Horinouchi; T Wakagi

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Nov. 2004, Peer-reviewed

  
• Molecular evolution of the ATPase subunit of three archaeal sugar ABC transporters

  H Imamura; BS Jeon; T Wakagi

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Jun. 2004, Peer-reviewed

  
• The F subunit of Thermus thermophilus V-1-ATPase promotes ATPase activity but is not necessary for rotation

  H Imamura; C Ikeda; M Yoshida; K Yokoyama

  JOURNAL OF BIOLOGICAL CHEMISTRY, Apr. 2004, Peer-reviewed

  
• Crystal structure of a central stalk subunit C and reversible association/dissociation of vacuole-type ATPase

  M Iwata; H Imamura; E Stambouli; C Ikeda; M Tamakoshi; K Nagata; H Makyio; B Hankamer; J Barber; M Yoshida; K Yokoyama; S Iwata

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Jan. 2004, Peer-reviewed

  
• Subunit arrangement in V-ATPase from Thermus thermophilus

  K Yokoyama; K Nagata; H Imamura; S Ohkuma; M Yoshida; M Tamakoshi

  JOURNAL OF BIOLOGICAL CHEMISTRY, Oct. 2003, Peer-reviewed

  
• Identification and molecular characterization of a novel type of alpha-galactosidase from Pyrococcus furiosus

  JFT Van Lieshout; CH Verhees; TJG Ettema; S van der Sar; H Imamura; H Matsuzawa; J van der Oost; WM De Vos

  BIOCATALYSIS AND BIOTRANSFORMATION, Aug. 2003, Peer-reviewed

  
• Unique metal dependency of cytosolic ot-mannosidase from Thermotoga maritima, a hyperthermophilic bacterium

  M Nakajima; H Imamura; H Shoun; T Wakagi

  ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, Jul. 2003, Peer-reviewed

  
• Rotation of the proteolipid ring in the V-ATPase

  K Yokoyama; M Nakano; H Imamura; M Yoshida; M Tamakoshi

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jul. 2003, Peer-reviewed

  
• Crystal structures of 4-alpha-glucanotransferase from Thermococcus litoralis and its complex with an inhibitor

  H Imamura; S Fushinobu; M Yamamoto; T Kumasaka; BS Jeon; T Wakagi; H Matsuzawa

  JOURNAL OF BIOLOGICAL CHEMISTRY, May 2003, Peer-reviewed

  
• Evidence for rotation of V-1-ATPase

  H Imamura; M Nakano; H Noji; E Muneyuki; S Ohkuma; M Yoshida; K Yokoyama

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Mar. 2003, Peer-reviewed

  
• Barrel structures of glycosyl hydrolases: crystal structures of family 42 and 57 enzymes

  S. Fushinobu; M. Hidaka; H. Imamura

  J. Struct. Biol. Sakabe Project., 2003

  
• 糖質加水分解酵素のバレル構造：ファミリー42と57のX線結晶構造解析

  伏信進矢; 日高將文; 今村博臣

  構造生物, 2003

  
• Identification of the catalytic residue of Thermococcus litoralis 4-alpha-glucanotransferase through mechanism-based labeling

  H Imamura; S Fushinobu; BS Jeon; T Wakagi; H Matsuzawa

  BIOCHEMISTRY, Oct. 2001, Peer-reviewed

  
• Reaction Mechanism and Crystal Structure of 4-α-Glucanotransferase from a Hyperthermophilic Archaeon, Thermococcus litoralis

  IMAMURA Hiromi; FUSHINOBU Shinya; YAMAMOTO Masaki; KUMASAKA Takashi; WAKAGI Takayoshi; MATSUZAWA Hiroshi

  Journal of Applied Glycoscience, 2001

  
• High level expression of Thermococcus litoralis 4-alpha-glucanotransferase in a soluble form in Escherichia coli with a novel expression system involving minor arginine tRNAs and GroELS

  H Imamura; BS Jeon; T Wakagi; H Matsuzawa

  FEBS LETTERS, Sep. 1999, Peer-reviewed

  
• Cloning of the gene for inorganic pyrophosphatase from a thermoacidophilic archaeon, Sulfolobus sp. strain 7, and overproduction of the enzyme by coexpression of tRNA for Arginine rare codon

  T Wakagi; T Oshima; H Imamura; H Matsuzawa

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Dec. 1998, Peer-reviewed

  

Misc.

• Visualization of branched-chain amino acids in single living cells

  今村博臣

  実験医学, 2022

  
• Dynamics of ATP Levels in Single Dying Apoptotic Cells

  今村博臣

  生物物理(Web), 2022

  
• プローブ 60.蛍光タンパク質 iii.ATPプローブ-細胞内エネルギー通貨の可視化技術

  今村博臣

  実験医学, 2018

  
• Cardiac Energetics Re-evaluated by in Vivo Visualization of ATP Levels

  Norimichi Koitabashi; Masamichi Yamamoto; Takayuki Musashi; Mai Samejima; Tatsuya Iso; Hiromi Imamura; Masahiko Kurabayashi

  JOURNAL OF CARDIAC FAILURE, Oct. 2015, Peer-reviewed

  
• 糖質加水分解酵素のバレル構造: ファミリー42と57のX線結晶構造解析

  伏信進矢; 日高将文; 今村博臣; 山本雅貴; 熊坂崇; 松沢洋; 若木高善; 祥雲弘文

  日本農芸化学会大会講演要旨集, 2003

  
• 微生物環境応答システムの速度論的解析と応用開発

  日高將文; 後藤愛那; 清水泰希; 南澤究; 今村博臣; 内田隆史

  日本農芸化学会大会講演要旨集(Web), 2016

  
• 腎障害とATP in vivo imaging

  山本伸也; 三井亜希子; 山本恵則; 今村博臣; 山本正道; 柳田素子

  日本腎臓学会誌, 2017

  
• 明らかになってきたATPの新しい役割-細胞のエネルギー運搬役の裏稼業-

  今村博臣

  現代化学, 2022

  
• 細胞多様性解明に資する光技術-見て,動かす II.見えなかったものを視る ATPバイオセンサーを用いた生細胞内ATP濃度の可視化・計測

  吉田有希; 今村博臣

  生体の科学, 2017

  
• “誰も知らない”出会いを支えるテクノロジー〈“代謝”イメージング〉6.細胞内のATPがみたい-ライブイメージングで明らかになってきた細胞内ATP濃度の時空間動態

  今村博臣

  実験医学, 2014

  
• 生命動態システム科学 I.全量生物学 1.分子・細胞の計測 3)分子機能計測(1)ATPバイオセンサーを使って見えてきたこと

  今村博臣

  生体の科学, 2014

  
• 古典的代謝経路の新しい側面 生細胞内ATP濃度イメージング

  今村博臣

  生体の科学, 2014

  
• 二つの回転分子モータータンパク質;V-ATPaseとF₀F₁の共通性と多様性

  今村博臣; 横山謙

  生化学, 2007

  
• 腎スライス培養を用いたATPイメージング系の樹立と各ネフロンセグメントにおけるATP産生機構の検討

  山本 恵則; 高橋 昌宏; 山本 伸也; 今村 博臣; 山本 正道; 柳田 素子

  日本腎臓学会誌, May 2019

  
• MPTPパーキンソン病モデルにおけるATP制御薬の発症緩和効果

  中野将希; 今村博臣; 笹岡紀男; 山本正道; 上村紀仁; 首藤敏之; 渕上智弘; 高橋良輔; 垣塚彰

  日本生体エネルギー研究会討論会講演要旨集, 05 Dec. 2017

  
• Imaging of intracellular ATP level revealed pannexin1-mediated programmed ATP decrease in apoptosis

  H. Imamura; S. Sakamoto; T. Yoshida; A. Kakizuka

  EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Jul. 2017

  
• ミトコンドリア・メディスン 腎障害とATP in vivo imaging

  山本 伸也; 三井 亜希子; 山本 恵則; 今村 博臣; 山本 正道; 柳田 素子

  日本腎臓学会誌, Apr. 2017

  
• 腎発生過程におけるエネルギー代謝の検討

  三井 亜希子; 山本 伸也; 山本 恵則; 今村 博臣; 山本 正道; 柳田 素子

  日本腎臓学会誌, Apr. 2017

  
• ATP制御薬による細胞内ATP維持はMPTPパーキンソン病モデルにおいて発症を緩和する

  中野将希; 今村博臣; 笹岡紀男; 山本正道; 上村紀仁; 首藤敏之; 首藤敏之; 渕上智弘; 高橋良輔; 垣塚彰

  日本生化学会大会(Web), 2017

  
• 脂質代謝・フリーラジカルと腎 腎臓におけるエネルギー代謝のin vivo imaging

  山本 伸也; 中村 仁; 三井 亜希子; 今村 博臣; 山本 正道; 柳田 素子

  日本腎臓学会誌, May 2016

  
• 胎生期腎臓のATPイメージング

  三井 亜希子; 山本 伸也; 中村 仁; 山本 恵則; 今村 博臣; 山本 正道; 柳田 素子

  日本腎臓学会誌, May 2016

  
• 尿細管上皮細胞内ATPのin vivo imaging

  山本 伸也; 中村 仁; 三井 亜希子; 山本 恵則; 今村 博臣; 山本 正道; 柳田 素子

  日本腎臓学会誌, May 2016

  
• 一細胞計測で見えてきたバクテリア細胞内ATP濃度の多様性

  柳沼秀幸; 柳沼秀幸; 柳沼秀幸; 河合信之輔; 河合信之輔; 田端和仁; 冨山佳祐; 垣塚彰; 小松崎民樹; 岡田康志; 野地博行; 野地博行; 今村博臣; 今村博臣

  日本細胞生物学会大会要旨集, Jun. 2015

  
• Macromolecular Crowding in the Cytosol: Underappreciated or Overestimated?

  Joost Groen; David Foschepoth; Arnold J. Boersma; Hiromi Imamura; Hans A. Heus; Wilhelm T. S. Huck

  BIOPHYSICAL JOURNAL, Jan. 2015

  
• 定量的ATPイメージングから明らかになった集団内における単一大腸菌細胞内ATP濃度の多様性

  柳沼秀幸; 河合信之輔; 田端和仁; 冨山佳祐; 垣塚彰; 小松崎民樹; 野地博行; 今村博臣

  日本生体エネルギー研究会討論会講演要旨集, 11 Dec. 2014

  
• 細胞外ATPダイナミクスの解明に向けた蛍光ATPバイオセンサーQUEENの反応高速化と光安定化

  岩切竜太; 垣塚彰; 今村博臣

  日本生体エネルギー研究会第40回討論会（ポスター）, Dec. 2014, Peer-reviewed

  
• 2SAP-04 Heterogeneity in ATP Concentrations in a Single Bacterial Cell Population Revealed by Quantitative Single-cell Imaging(Scenario of functions from minority and number fluctuations,Symposium,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))

  Yaginuma Hideyuki; Kawai Shinnosuke; Tabata Kazuhito; Tomiyama Keisuke; Kakizuka Akira; Komatsuzaki Tamiki; Okada Yasushi; Noji Hiroyuki; Imamura Hiromi

  Seibutsu Butsuri, 2014

  
• 低酸素により誘導されるミトコンドリアタンパク質MENTはATP合成を促進し低酸素から心臓を保護する

  木岡 秀隆; 加藤 久和; 藤川 誠; 鈴木 俊治; 今村 博臣; 野地 博行; 吉田 賢右; 北風 政史; 朝野 仁裕; 小室 一成; 高島 成二

  日本臨床分子医学会学術総会プログラム・抄録集, Apr. 2013

  
• Imaging of intracellular ATP dynamics in single apoptotic cells.

  Shu-ichiro Sakamoto; Akira Kakizuka; Hiromi Imamura

  The 11th International Student Seminar, 06 Mar. 2013, Peer-reviewed

  
• バクテリア細胞内ATP濃度の一細胞計測

  柳沼秀幸; 柳沼秀幸; 河合信之輔; 田端和仁; 田端和仁; 冨山佳祐; 垣塚彰; 小松崎民樹; 今村博臣; 野地博行; 野地博行

  日本生化学会大会(Web), 2013

  
• 低酸素により誘導されるミトコンドリアタンパク質MENTはATP合成酵素と結合しミトコンドリアATP合成を促進する

  加藤久和; 木岡秀隆; 藤川誠; 鈴木俊治; 今村博臣; 吉田賢右; 朝野仁裕; 高島成二

  日本分子生物学会年会プログラム・要旨集(Web), 2013

  
• アポトーシスにおける細胞内ATPの挙動の解析

  坂本修一朗; 野地博行; 垣塚彰; 今村博臣

  日本生体エネルギー研究会（ポスター発表）, 22 Dec. 2012, Peer-reviewed

  
• 蛍光ATPバイオセンサーを用いたインスリン分泌細胞内ATPのダイナミクスの計測

  田中喬; 長嶋一昭; 稲垣暢也; 野地博行; 垣塚彰; 今村博臣

  日本生体エネルギー研究会（ポスター発表）, 22 Dec. 2012, Peer-reviewed

  
• Fluorescent ATP biosensor optimized for imaging of cells from non-vertebrate model organisms

  Hiromi Imamura; Taiichi Tsuyama; Tadashi Uemura; Akira Kakizuka

  International Symposia "New Frontiers of Metabolism Research in Biomedical Sciences", 28 Sep. 2012, Peer-reviewed

  
• Genetically-encoded ATP biosensor for low temperatures

  Hiromi Imamura; Taiichi Tsuyama; Jun-ichi Kishikawa; Hiroyuki Noji; Akira Kakizuka; Ken Yokoyama; Tadashi Uemura

  European Bioenergetic Conference 2012（ポスター発表）, 19 Sep. 2012, Peer-reviewed

  
• 蛍光プローブを用いた膵β細胞内ATPおよびCa2+濃度同時測定の試み

  長嶋 一昭; 今村 博臣; 田中 喬; 田中 大祐; 稲垣 暢也

  糖尿病, Apr. 2012

  
• Simultaneous ATP and Ca2+ imaging of a single insulin-secreting islet cell.

  Takashi Tanaka; Kazuaki Nagashima; Nobuya Inagaki; Hiroyuki Noji; Akira Kakizuka; Hiromi Imamura

  The 10th International Student Seminar, 05 Mar. 2012, Peer-reviewed

  
• Pannexin 1は細胞内ATPレベルを制御することでアポトーシスの進行に重要な役割を果たす

  坂本修一朗; 野地博行; 垣塚彰; 今村博臣

  第37回日本生体エネルギー研究会シンポジウム（ポスター発表）, 21 Dec. 2011, Peer-reviewed

  
• ATPバイオセンサー(GO-ATeam)を用いた膵島β細胞のライブイメージング

  田中喬; 長嶋一昭; 稲垣暢也; 野地博行; 垣塚彰; 今村博臣

  第７回日本ケミカルバイオロジー学会年会（ポスター発表）, 21 Dec. 2011, Peer-reviewed

  
• 飢餓条件下におけるVCPタンパク質の機能解析

  小池雅昭; 今村博臣; 垣塚彰

  第84回日本生化学会大会, 24 Sep. 2011, Peer-reviewed

  
• 赤方化した蛍光ATPプローブを用いた単一細胞内のATPとカルシウムイオンの同時イメージング

  中野 雅裕; 今村 博臣; 永井 健治; 野地 博行

  バイオイメージング, 10 Aug. 2011

  
• 線虫Caenorhabditis elegansの加齢に伴うATP濃度変化の測定

  岸川淳一; 藤川誠; 今村博臣; 安田佳代; 野地博之; 石井直明; 三谷昌平; 横山謙; 横山謙

  生化学, 2011

  
• 線虫Caenorhabditis elegansを用いた個体ATPイメージング

  岸川淳一; 藤川誠; 今村博臣; 安田佳代; 野地博行; 石井直明; 三谷昌平; 横山謙; 横山謙

  日本生体エネルギー研究会討論会講演要旨集, 2011

  
• Simultaneous ratiometric imaging of ATP and Ca2+ concentrations inside single living cells

  Hiromi Imamura; Masahiro Nakano; Hiroyuki Noji

  BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Jul. 2010

  
• ATP Gradient Across the Innermitochondrial Membrane

  Hiromi Imamura; Hiroyuki Noji

  BIOPHYSICAL JOURNAL, Jan. 2010

  
• Mechanochemical coupling of F-1-ATPase and intracellular ATP imaging

  Hiroyuki Noji; Rikiya Watanabe; Daichi Okuno; Hiromi Imamura

  BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Jul. 2008

  
• Analysis of stepwise rotation of V-1 motor at low ATP concentrations

  H Imamura; M Yoshida; K Yokoyama

  BIOPHYSICAL JOURNAL, Jan. 2005

  
• Purification and crystallization of the V-ATPase subunits from thermus thermophilus

  H Makyio; H Imamura; C Ikeda; M Iwata; M Yoshida; K Yokoyama; S Iwata

  BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2004

  
• Crystal structure of a central stalk subunit C and reversible association/dissociation of V-ATPase

  M Iwata; H Imamura; E Stambouli; M Tamakoshi; H Makyio; J Barber; M Yoshida; K Yokoyama; S Iwata

  BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2004

  
• The role of the F subunit of thermus thermophilus V-ATPase on ATP hydrolysis and rotation

  H Imamura; C Ikeda; M Yoshida; K Yokoyama

  BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2004

  
• V type ATPase/synthase from thermus thermophilus

  K Yokoyama; M Iwata; M Hisayoshi; Y Masasuke; H Imamura

  BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2004

  

Presentations

• 緑色蛍光タンパク質由来赤色蛍光タンパク質の創出

  今村博臣; 大坪史歩; 竹川宣宏; 今田勝巳

  第21回日本蛋白質科学会年会, 18 Jun. 2021

  
• イメージングで生細胞内の代謝物動態を明らかにする

  今村博臣

  農芸化学会2021年度大会, 21 Mar. 2021, Invited

  
• アポトーシスにおいてパネキシン１によって駆動されるプログラムされたATP減少の単一細胞イメージング

  今村博臣

  第12回光塾, 01 Dec. 2020

  
• Genetically encoded fluorescent biosensors for understanding of metabolism at single cell level

  Hiromi Imamura

  2nd WPI-NanoLSI Special Seminar –Frontiers in Chem-Bio–, 30 Jan. 2020, Invited

  
• 蛍光変化のダイナミックレンジを増大させたFRET型バイオセンサー開発

  米満茜; 垣塚彰; 今村博臣

  日本生体エネルギー研究会第45回討論会, 21 Dec. 2019

  
• Imaging of metabolites inside living cells

  Hiromi Imamura

  Academia Sinica – Kyoto University Bilateral Symposium, 18 Dec. 2019, Invited

  
• Genetically encoded fluorescent biosensor for branched-chain amino acids

  IMAMURA Hiromi; YOSHIDA Tomoki; NAKAJIMA Hitomi; TAKAHASHI Sena; KAKIZUKA Akira

  第５７回日本生物物理学会年会, 26 Sep. 2019, Invited

  
• 蛍光バイオセンサーで解き明かす細胞内代謝

  今村 博臣

  第７１回日本生物工学会大会, 18 Sep. 2019, Invited

  
• 蛍光バイオセンサーによる生細胞内代謝物イメージング

  今村 博臣

  日本分析化学会 第６８年会, 13 Sep. 2019, Invited

  
• 新規発光型ATPバイオセンサーを用いた細胞内および細胞外ATPダイナミクスの定量的観測系の構築

  松岡 篤志; 吉田 有希; 垣塚 彰; 今村 博臣

  日本生体エネルギー研究会 第44回討論会, Dec. 2018

  
• Single-cell imaging of intracellular ATP dynamics during apoptosis using a caspase-resistant FRET-based biosensor

  IMAMURA Hiromi; SAKAMOTO Shuichiro; YOSHIDA Tomoki; KAKIZUKA Akira

  International Meeting on Optical Biosensors, Nov. 2018

  
• 出芽酵母の細胞内ATP変動を制御する仕組み

  高稲 正勝; 今村 博臣; 吉田 知史

  日本生体エネルギー研究会 第43回討論会, Dec. 2017

  
• MPTPパーキンソン病モデルにおけるATP制御薬の発症緩和効果

  中野 将希; 今村 博臣; 笹岡 紀男; 山本 正道; 上村 紀仁; 首藤 敏之; 渕上 智弘; 高橋 良輔; 垣塚 彰

  日本生体エネルギー研究会 第43回討論会, Dec. 2017

  
• FRET型蛍光バイオセンサーを用いたミトコンドリア分岐鎖アミノ酸輸送体の探索

  中嶋 瞳; 吉田 有希; 垣塚 彰; 今村 博臣

  日本生体エネルギー研究会 第43回討論会, Dec. 2017

  
• ツメガエル卵細胞におけるATPの役割の検証

  井尻 貴之; 岸川 淳一; 今村 博臣; 上野 秀一; 岩尾 康宏; 横山 謙; 佐藤 賢一

  日本生体エネルギー研究会 第43回討論会, Dec. 2017

  
• ルシフェラーゼ断片の相補的再構成を用いた新規発光ATPバイオセンサーの開発

  松岡 篤志; 垣塚 彰; 今村 博臣

  日本生体エネルギー研究会 第43回討論会, Dec. 2017

  
• FRET型ATPセンサーを用いたショウジョウバエ神経細胞におけるATPの濃度と消費速度の可視化

  津山 泰一; 坪内 朝子; 碓井 理夫; 今村 博臣; 上村 匡

  日本生体エネルギー研究会 第43回討論会, Dec. 2017

  
• Imaging of intracellular ATP level revealed pannexin1-mediated programmed ATP decrease in apoptosis

  IMAMURA Hiromi; SAKAMOTO Shuichiro; YOSHIDA Tomoki; KAKIZUKA Akira

  19th International Union for Pure and Applied Biophysics Congress / 11th European Biophysics Congress, Jul. 2017

  

Awards

• Dec. 2007

  日本生物物理学会, 日本生物物理学会　若手奨励賞
• 22 Apr. 2016

  NAGASE Science Technology Foundation, Nagase Foundation Award
• 22 Feb. 2019

  公益財団法人 中谷医工計測技術振興財団, 中谷賞奨励賞

External funds: Kakenhi

• Construction of photosynthetic zebrafish

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 38:Agricultural chemistry and related fields

  Mie University

  Yasuhito Shimada

  From 30 Jul. 2020, To 31 Mar. 2022, Project Closed

  ゼブラフィッシュ;光合成;光共生;ATP

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K21273/
• 光熱変換を利用した細胞操作技術の開発

  Grant-in-Aid for Transformative Research Areas (B)

  Transformative Research Areas, Section (III)

  Kyoto University

  今村 博臣

  From 02 Oct. 2020, To 31 Mar. 2023, Granted

  色素タンパク質;蛍光タンパク質;温度生物学;TRPチャネル

  https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-20H05757/
• Next generation non-invasive deep biological manipulation by combining biomolecular engineering and low physical energy logistics

  Grant-in-Aid for Transformative Research Areas (B)

  Transformative Research Areas, Section (III)

  The University of Tokyo

  井上 圭一

  From 02 Oct. 2020, To 31 Mar. 2023, Granted

  深部生体操作;光熱変換;超音波;磁場;低物理エネルギーロジスティクス

  https://kaken.nii.ac.jp/grant/KAKENHI-ORGANIZER-20H05755/
• 試験管内分子進化と立体構造解析に基づいた赤色蛍光タンパク質の系譜拡張

  Grant-in-Aid for Scientific Research (B)

  Basic Section 43040:Biophysics-related

  Kyoto University

  今村 博臣

  From 01 Apr. 2020, To 31 Mar. 2023, Granted

  蛍光タンパク質;試験管内分子進化;X線結晶構造解析;イメージング

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20H03221/
• Study on the remodeling of energy metabolism of cancer cells with a novel technology of isolating cells based on ATP concentrations

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields

  Kyoto University

  Hiromi Imamura

  From 28 Jun. 2019, To 31 Mar. 2022, Project Closed

  解糖系;酸化的リン酸化;ATP;ワールブルグ効果;CRISPR;FRET;蛍光バイオセンサー;フローサイトメトリー;エネルギー代謝;フローサイトメーター

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K22386/
• 人工生体分子反応回路を埋め込んだ紙基板バイオアッセイ法の開発

  Grant-in-Aid for Scientific Research (C)

  Basic Section 37010:Bio-related chemistry

  Osaka University

  松浦 秀幸

  From 01 Apr. 2018, To 31 Mar. 2021, Declined

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K05317/
• Study of mitochondrial transporters branched-chain amino acids using genetically encoded fluorescent biosensors

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Hiromi Imamura

  From 01 Apr. 2017, To 31 Mar. 2020, Project Closed

  分岐鎖アミノ酸;BCAA;イメージング;FRET;蛍光;トランスポーター;siRNA;蛍光バイオセンサー;SLC25A44;ミトコンドリア;ライブイメージング;バイオセンサー

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17H03654/
• Quantitative analysis of ATP dynamics in photosynthetic organisms by a novel bioluminescence ATP biosensor

  Grant-in-Aid for Challenging Exploratory Research

  Kyoto University

  Hiromi Imamura

  From 01 Apr. 2016, To 31 Mar. 2018, Project Closed

  生物発光;BRET;イメージング;光合成;ATP;ライブイメージング;エネルギー代謝

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K14709/
• 遺伝子コードされたFRET型酸素バイオセンサーの開発

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Biological Sciences

  Kyoto University

  今村 博臣

  From 01 Apr. 2015, To 31 Mar. 2017, Project Closed

  酸素;FRET;抗体;バイオセンサー;バイオテクノロジー;イメージング

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-15H01401/
• Energy dynamics during kidney development, injury and regeneration

  Grant-in-Aid for Challenging Exploratory Research

  Kyoto University

  Motoko Yanagita

  From 01 Apr. 2014, To 31 Mar. 2015, Project Closed

  エネルギー代謝

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26670430/
• Development of efficient protein production system by photo-driven ATP regenerating vesicle

  Grant-in-Aid for Challenging Exploratory Research

  Kobe University

  Kiyotaka HARA

  From 01 Apr. 2014, To 31 Mar. 2015, Project Closed

  ATP;Protein;Probe;Photo;Chaperone;Structure;無細胞合成系;シャペロン;蛍光

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26630430/
• Spatio-temporal analysis of extracellular ATP signaling of pancreatic islets

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Hiromi Imamura

  From 01 Apr. 2014, To 31 Mar. 2017, Project Closed

  ATP;バイオセンサー;蛍光タンパク質;ブリン受容体;インスリン;プリン受容体;ライブイメージング;生物発光;BRET;ATPシグナル;イメージング

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26291027/
• Prevention, diagnosis and drug discovery of aging diseases using Pin1 related molecules

  Grant-in-Aid for Scientific Research (A)

  Tokyo Kasei University;Tohoku University

  Takafumi Uchida

  From 01 Apr. 2014, To 31 Mar. 2019, Project Closed

  プロリン異性化酵素;Pin1;Gas7;FRET;癌;アルツハイマー病;ポリフェノール;tau;Myc;健康長寿;肥満;血小板;心臓疾患;幹細胞;酵素;再生医学;脳神経疾患;食品;加齢疾患;骨粗鬆症;微小管;シグナル伝達;硝酸;FOXO3;MDR;AMPK;生体機能利用;Gas7b;Tau;NO

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26252064/
• Functional analysis of pancreatic beta-cells in metabolic disorders using the fluorescent ATP probe gene transgenic mouse

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  KAZUAKI NAGASHIMA

  From 01 Apr. 2013, To 31 Mar. 2016, Project Closed

  ATP;KATPチャネル;糖代謝異常

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25461346/
• Development of methods to visualize small conformational change of proteins inside cells

  Grant-in-Aid for Challenging Exploratory Research

  Kyoto University

  Hiromi IMAMURA

  From 01 Apr. 2012, To 31 Mar. 2014, Project Closed

  バイオイメージング;FRET;タンパク質動態;バイオセンサー;抗体;アセチルCoA;蛍光タンパク質;イメージング

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24657101/
• Development of neuroprotective treatment for incurable ocular diseases

  Grant-in-Aid for Scientific Research (A)

  Kyoto University

  Nagahisa YOSHIMURA

  From 31 Oct. 2012, To 31 Mar. 2015, Project Closed

  神経保護;眼難治疾患;眼細胞生物学

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24249082/
• Comprehensive quantification and visualization of energy metabolism in cell competition between dying and living cells.

  Grant-in-Aid for Challenging Exploratory Research

  Waseda University

  Nanami MATSUDA;Nanami Senoo-Matsuda

  From 01 Apr. 2011, To 31 Mar. 2013, Project Closed

  細胞競合;エネルギー代謝;Myc;p53;ショウジョウバエ;メタボローム;可視化;細胞間相互作用

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23657150/
• Analyses of the function and regulation of VCP, a major ATPase in the cells

  Grant-in-Aid for Scientific Research (A)

  Kyoto University

  Akira KAKIZUKA

  From 31 May 2011, To 31 Mar. 2014, Project Closed

  ATP;ATPase;VCP;ERストレス;細胞死;網膜色素変性症;緑内障;酵素;胞・組織;ストレス;蛋白質;阻害剤;繊維状構造物;グルコース代謝;飢餓;リン脂質

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23249016/
• Analysis of the roles of intracellular ATP during apoptosis by using the ATP imaging technique.

  Grant-in-Aid for Young Scientists (A)

  Kyoto University

  Hiromi IMAMURA

  From 01 Apr. 2010, To 31 Mar. 2013, Project Closed

  細胞生物学;細胞死;FRET;イメージング;エネルギー代謝;ATP放出;ライブイメージング;共鳴エネルギー異動;バイオセンサー;ミトコンドリア

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22687011/
• Real-time measurement of intracellular ATP concentration using novel fluorescent ATP probe.

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Kazuaki NAGASHIMA

  From 01 Apr. 2010, To 31 Mar. 2013, Project Closed

  エネルギー;糖質代謝異常;ATP;K_ATP チャネル;糖尿病;KATPチャネル;糖代謝;K_チャネル

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590977/
• 細胞内アミノ酸ダイナミクスのリアルタイム可視化によるがん幹細胞維持シグナルの解明

  Grant-in-Aid for Scientific Research (C)

  Basic Section 50010:Tumor biology-related

  Kyoto University

  松浦 顕教

  From 01 Apr. 2022, To 31 Mar. 2025, Granted

  アミノ酸;代謝;がん幹細胞;FRETバイオセンサー

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K07188/
• 細胞死においてATP濃度の低下を誘導する新規因子の探索

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields

  Kyoto University

  今村 博臣

  From 30 Jun. 2022, To 31 Mar. 2024, Granted

  アポトーシス;ATP;CRISPR;バイオセンサー

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K19292/

Education

Teaching subject(s)

• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar D

  2009, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Experiments in Systemic Life Sciences

  5013, Spring, Graduate School of Biostudies, 4
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar C

  2008, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar B

  2007, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar B

  2007, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Experiments in Systemic Life Sciences

  5013, Fall, Graduate School of Biostudies, 4
• From 01 Apr. 2022, To 31 Mar. 2023

  Introduction to Biology and Life Science

  N484, Spring, Institute for Liberal Arts and Sciences, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar D

  2009, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Life Science: From Basics to Applications, from Molecular Biology to Systems Biology

  1026, Spring, Graduate School of Biostudies, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar C

  2008, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar A

  2006, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar A

  2006, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Fundamental Technologies for Advanced Life Science II

  N453, Fall, Institute for Liberal Arts and Sciences, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Global Frontier in Life Science A

  1018, Spring, Graduate School of Biostudies, 2
• From Apr. 2011, To Mar. 2012

  Perspectives on Life Science A

  Spring, 全学共通科目
• From Apr. 2012, To Mar. 2013

  Perspectives on Life Science A

  Spring, 全学共通科目
• From Apr. 2012, To Mar. 2013

  Direct observation of the protein shape and life phenomenon

  Spring, 全学共通科目
• From Apr. 2013, To Mar. 2014

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2013, To Mar. 2014

  Study of Life Science I

  Spring, 全学共通科目
• From Apr. 2013, To Mar. 2014

  In order to Know, Observe, and Create our Life : The Molecular Science for Life

  Spring, 全学共通科目
• From Apr. 2014, To Mar. 2015

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2014, To Mar. 2015

  Molecular and Cellular Biology

  Fall, 総合生存学館
• From Apr. 2014, To Mar. 2015

  Study of Life Science I

  Spring, 全学共通科目
• From Apr. 2014, To Mar. 2015

  Life Sciences: Knowledge, Observation, and Creation of a Life

  Spring, 全学共通科目
• From Apr. 2015, To Mar. 2016

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2015, To Mar. 2016

  Molecular and Cellular Biology

  Fall, 総合生存学館
• From Apr. 2015, To Mar. 2016

  Study of Life Science I

  Spring, 全学共通科目
• From Apr. 2015, To Mar. 2016

  Understanding life: From molecule to ecosystem.

  Spring, 全学共通科目
• From Apr. 2016, To Mar. 2017

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2016, To Mar. 2017

  Molecular and Cellular Biology

  Fall, 総合生存学館
• From Apr. 2017, To Mar. 2018

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2017, To Mar. 2018

  Molecular and Cellular Biology

  Fall, 総合生存学館
• From Apr. 2018, To Mar. 2019

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2018, To Mar. 2019

  Introduction to Biology and Life Science

  Spring, 全学共通科目
• From Apr. 2019, To Mar. 2020

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2019, To Mar. 2020

  ILAS Seminar

  Spring, 全学共通科目
• From Apr. 2020, To Mar. 2021

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2020, To Mar. 2021

  ILAS Seminar :Basics in Medical Biology

  Spring, 全学共通科目
• From Apr. 2020, To Mar. 2021

  Life Science: From Basics to Applications, from Molecular Biology to Systems Biology

  Spring, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  ILAS Seminar :Basics in Medical Biology

  Spring, 全学共通科目
• From Apr. 2021, To Mar. 2022

  Life Science: From Basics to Applications, from Molecular Biology to Systems Biology

  Spring, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Advanced Experiments in Systemic Life Sciences

  Spring, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Advanced Experiments in Systemic Life Sciences

  Fall, 生命科学研究科

Part-time lecturer

• From 17 Sep. 2022, To 20 Jan. 2023

  龍谷大学
• From 01 Apr. 2022, To 30 Jun. 2022

  東京大学
• From 20 Sep. 2017, To 30 Jan. 2018

  龍谷大学
• From 01 Apr. 2018, To 30 Jun. 2018

  東京大学, 大学院農学生命科学研究科
• From 20 Sep. 2018, To 21 Jan. 2019

  龍谷大学
• From 20 Sep. 2019, To 20 Jan. 2020

  龍谷大学
• From 01 Apr. 2020, To 30 Jun. 2020

  東京大学, 大学院農学生命科学研究科
• From 18 Sep. 2020, To 19 Jan. 2021

  龍谷大学
• From 18 Sep. 2021, To 19 Jan. 2022

  龍谷大学

Administration

School management (title, position)

• From 01 Apr. 2022, To 30 Sep. 2022

  男女共同参画推進センター　広報専門部会
• From 01 Apr. 2015, To 31 Mar. 2021

  京都大学放射線予防小委員会委員
• From 01 Oct. 2016, To 30 Sep. 2022

  男女共同参画推進委員会委員
• From 01 Oct. 2016, To 30 Sep. 2022

  男女共同参画推進センター　広報・相談・社会連携事業ワーキンググループ委員

Faculty management (title, position)

• From 01 Apr. 2015, To 31 Jul. 2019

  RI委員会委員
• From 01 Apr. 2015, To 31 Jul. 2019

  先端科学研究棟RI施設主任者
• From 01 Apr. 2017

  安全・環境保全委員会委員
• From 01 Aug. 2019, To 31 Mar. 2021

  放射線障害防止委員会２号（先端棟）（主任者）
• From 01 Apr. 2021, To 31 Mar. 2022

  放射線障害防止委員会３号
• From 01 Apr. 2019

  男女共同参画推進アクションプラン推進委員会　委員