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Sakanaka, Mikiyasu

Graduate School of Biostudies Program-Specific Associate Professor

Sakanaka, Mikiyasu
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    Last Updated :2024/02/21

    Basic Information

    Professional Memberships

    • 日本ゲノム微生物学会
    • American Society for Microbiology
    • ビフィズス菌研究会
    • 日本農芸化学会
    • 腸内細菌学会
    • 日本乳酸菌学会

    Academic Degree

    • 修士(農学)(北海道大学)
    • 博士(農学)(北海道大学)

    Research History

    • From Oct. 2020, To Present
      京都大学, 大学院生命科学研究科, 特定准教授
    • From Sep. 2020, To Sep. 2020
      京都大学, 大学院生命科学研究科, 特定研究員
    • From Sep. 2018, To Aug. 2020
      Technical University of Denmark, National Food Institute, Visiting Researcher (日本学術振興会 海外特別研究員)
    • From Apr. 2017, To Aug. 2018
      石川県立大学, 生物資源環境学部, 寄附講座助教
    • From Apr. 2015, To Mar. 2017
      石川県立大学, 生物資源環境学部, 特別研究員

    ID,URL

    researchmap URL

    list
      Last Updated :2024/02/21

      Research

      Research Interests

      • 乳児
      • 母乳
      • 多糖
      • アミノ酸
      • ビフィズス菌
      • 腸内細菌
      • 遺伝子操作
      • 糖質加水分解酵素
      • トランスポーター
      • オリゴ糖

      Research Areas

      • Life sciences, Ecology and environmental science
      • Life sciences, Molecular biology
      • Life sciences, Functional biochemistry
      • Life sciences, Applied biochemistry
      • Life sciences, Applied microbiology

      Papers

      • Preferential sugar utilization by bifidobacterial species
        Ryuta Murakami; Keisuke Yoshida; Mikiyasu Sakanaka; Tadasu Urashima; Jin-Zhong Xiao; Takane Katayama; Toshitaka Odamaki
        Microbiome Research Reports, Aug. 2023, Peer-reviewed
      • Lactose or milk oligosaccharide: which is significant among mammals?
        Tadasu Urashima; Risa Horiuchi; Mikiyasu Sakanaka; Takane Katayama; Kenji Fukuda
        Animal Frontiers, Jun. 2023, Peer-reviewed
      • Substrate recognition mode of a glycoside hydrolase family 42 β-galactosidase from Bifidobacterium longum subspecies infantis (BiBga42A) revealed by crystallographic and mutational analyses
        Aina Gotoh; Masafumi Hidaka; Haruko Sakurama; Mamoru Nishimoto; Motomitsu Kitaoka; Mikiyasu Sakanaka; Shinya Fushinobu; Takane Katayama
        Microbiome Research Reports, May 2023, Peer-reviewed
      • GH20 and GH84 β-N-acetylglucosaminidases with different linkage specificities underpin mucin O-glycan breakdown capability of Bifidobacterium bifidum
        Hiromi Takada; Toshihiko Katoh; Mikiyasu Sakanaka; Toshitaka Odamaki; Takane Katayama
        Journal of Biological Chemistry, May 2023, Peer-reviewed
      • N-Carbamoylputrescine amidohydrolase of Bacteroides thetaiotaomicron, a dominant species of the human gut microbiota
        Hiromi Shimokawa; Mikiyasu Sakanaka; Yuki Fujisawa; Hirokazu Ohta; Yuta Sugiyama; Shin Kurihara
        Biomedicines, Apr. 2023, Peer-reviewed, Lead author
      • A bacterial sulfoglycosidase highlights mucin O-glycan breakdown in the gut ecosystem.
        Toshihiko Katoh; Chihaya Yamada; Michael D Wallace; Ayako Yoshida; Aina Gotoh; Moe Arai; Takako Maeshibu; Toma Kashima; Arno Hagenbeek; Miriam N Ojima; Hiromi Takada; Mikiyasu Sakanaka; Hidenori Shimizu; Keita Nishiyama; Hisashi Ashida; Junko Hirose; Maria Suarez-Diez; Makoto Nishiyama; Ikuo Kimura; Keith A Stubbs; Shinya Fushinobu; Takane Katayama
        Nature Chemical Biology, Mar. 2023, Peer-reviewed
      • Gut bacterial aromatic amine production: aromatic amino acid decarboxylase and its effects on peripheral serotonin production
        Yuta Sugiyama; Yumiko Mori; Misaki Nara; Yusuke Kotani; Emiko Nagai; Hiroki Kawada; Mayu Kitamura; Rika Hirano; Hiromi Shimokawa; Akira Nakagawa; Hiromichi Minami; Aina Gotoh; Mikiyasu Sakanaka; Noriho Iida; Takashi Koyanagi; Takane Katayama; Shigefumi Okamoto; Shin Kurihara
        Gut Microbes, Dec. 2022, Peer-reviewed
      • Human milk oligosaccharide utilization in intestinal bifidobacteria is governed by global transcriptional regulator NagR
        Aleksandr A. Arzamasov; Aruto Nakajima; Mikiyasu Sakanaka; Miriam N. Ojima; Takane Katayama; Dmitry A. Rodionov; Andrei L. Osterman
        mSystems, Sep. 2022, Peer-reviewed
      • Priority effects shape the structure of infant-type Bifidobacterium communities on human milk oligosaccharides.
        Miriam N Ojima; Lin Jiang; Aleksandr A Arzamasov; Keisuke Yoshida; Toshitaka Odamaki; Jinzhong Xiao; Aruto Nakajima; Motomitsu Kitaoka; Junko Hirose; Tadasu Urashima; Toshihiko Katoh; Aina Gotoh; Douwe van Sinderen; Dmitry A Rodionov; Andrei L Osterman; Mikiyasu Sakanaka; Takane Katayama
        The ISME Journal, Jun. 2022, Peer-reviewed
      • A simple method that enhances minority species detection in the microbiota: 16S metagenome-DRIP (Deeper Resolution using an Inhibitory Primer)
        Aruto Nakajima; Keisuke Yoshida; Aina Gotoh; Toshihiko Katoh; Miriam N. Ojima; Mikiyasu Sakanaka; Jin-Zhong Xiao; Toshitaka Odamaki; Takane Katayama
        Microbiome Research Reports, May 2022, Peer-reviewed
      • Diversification of a fucosyllactose transporter within the genus Bifidobacterium.
        Miriam N Ojima; Yuya Asao; Aruto Nakajima; Toshihiko Katoh; Motomitsu Kitaoka; Aina Gotoh; Junko Hirose; Tadasu Urashima; Satoru Fukiya; Atsushi Yokota; Maher Abou Hachem; Mikiyasu Sakanaka; Takane Katayama
        Applied and Environmental Microbiology, Jan. 2022, Peer-reviewed, Corresponding author
      • Bifidobacterium species associated with breastfeeding produce aromatic lactic acids in the infant gut.
        Martin F Laursen; Mikiyasu Sakanaka; Nicole von Burg; Urs Mörbe; Daniel Andersen; Janne Marie Moll; Ceyda T Pekmez; Aymeric Rivollier; Kim F Michaelsen; Christian Mølgaard; Mads Vendelbo Lind; Lars O Dragsted; Takane Katayama; Henrik L Frandsen; Anne Marie Vinggaard; Martin I Bahl; Susanne Brix; William Agace; Tine R Licht; Henrik M Roager
        Nature Microbiology, Oct. 2021, Peer-reviewed
      • Next-generation prebiotic promotes selective growth of bifidobacteria, suppressing Clostridioides difficile
        Rika Hirano; Mikiyasu Sakanaka; Kazuto Yoshimi; Naohisa Sugimoto; Syogo Eguchi; Yuko Yamauchi; Misaki Nara; Shingo Maeda; Yuta Ami; Aina Gotoh; Takane Katayama; Noriho Iida; Tamotsu Kato; Hiroshi Ohno; Satoru Fukiya; Atsushi Yokota; Mamoru Nishimoto; Motomitsu Kitaoka; Hiroyuki Nakai; Shin Kurihara
        Gut Microbes, Sep. 2021, Peer-reviewed
      • Evolution of milk oligosaccharides: Origin and selectivity of the ratio of milk oligosaccharides to lactose among mammals
        Tadasu Urashima; Takane Katayama; Mikiyasu Sakanaka; Kenji Fukuda; Michael Messer
        Biochimica et Biophysica Acta (BBA) - General Subjects, Sep. 2021, Peer-reviewed
      • Ecological and molecular perspectives on responders and non-responders to probiotics and prebiotics.
        Miriam N Ojima; Keisuke Yoshida; Mikiyasu Sakanaka; Lin Jiang; Toshitaka Odamaki; Takane Katayama
        Current Opinion in Biotechnology, Aug. 2021, Peer-reviewed
      • Bifidobacterium response to lactulose ingestion in the gut relies on a solute-binding protein-dependent ABC transporter
        Keisuke Yoshida; Rika Hirano; Yohei Sakai; Moonhak Choi; Mikiyasu Sakanaka; Shin Kurihara; Hisakazu Iino; Jin-zhong Xiao; Takane Katayama; Toshitaka Odamaki
        Communications Biology, May 2021, Peer-reviewed
      • Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways
        Michael Jakob Pichler; Chihaya Yamada; Bashar Shuoker; Camila Alvarez-Silva; Aina Gotoh; Maria Louise Leth; Erwin Schoof; Toshihiko Katoh; Mikiyasu Sakanaka; Takane Katayama; Chunsheng Jin; Niclas G. Karlsson; Manimozhiyan Arumugam; Shinya Fushinobu; Maher Abou Hachem
        Nature Communications, Jul. 2020, Peer-reviewed
      • Development of a new chromogenic method for putrescine quantification using coupling reactions involving putrescine oxidase
        Yuta Sugiyama; Hirokazu Ohta; Rika Hirano; Hiromi Shimokawa; Mikiyasu Sakanaka; Takashi Koyanagi; Shin Kurihara
        Analytical Biochemistry, Mar. 2020, Peer-reviewed
      • Application of Recombinase-Based In Vivo Expression Technology to Bifidobacterium longum subsp. longum for Identification of Genes Induced in the Gastrointestinal Tract of Mice
        Hiroka Koguchi; Natsumi Ishigami; Mikiyasu Sakanaka; Kako Yoshida; Sayaka Hiratou; Mina Shimada; Satoru Fukiya; Kei Sonoyama; Atsushi Yokota
        Microorganisms, Mar. 2020, Peer-reviewed
      • Enzymatic Adaptation of Bifidobacterium bifidum to Host Glycans, Viewed from Glycoside Hydrolyases and Carbohydrate-Binding Modules
        Toshihiko Katoh; Miriam N. Ojima; Mikiyasu Sakanaka; Hisashi Ashida; Aina Gotoh; Takane Katayama
        Microorganisms, Mar. 2020, Peer-reviewed
      • Varied Pathways of Infant Gut-Associated Bifidobacterium to Assimilate Human Milk Oligosaccharides: Prevalence of the Gene Set and Its Correlation with Bifidobacteria-Rich Microbiota Formation
        Mikiyasu Sakanaka; Aina Gotoh; Keisuke Yoshida; Toshitaka Odamaki; Hiroka Koguchi; Jin-zhong Xiao; Motomitsu Kitaoka; Takane Katayama
        Nutrients, Dec. 2019, Peer-reviewed, Lead author
      • Evolutionary adaptation in fucosyllactose uptake systems supports bifidobacteria-infant symbiosis
        Mikiyasu Sakanaka; Morten Ejby Hansen; Aina Gotoh; Toshihiko Katoh; Keisuke Yoshida; Toshitaka Odamaki; Hiroyuki Yachi; Yuta Sugiyama; Shin Kurihara; Junko Hirose; Tadasu Urashima; Jin-zhong Xiao; Motomitsu Kitaoka; Satoru Fukiya; Atsushi Yokota; Leila Lo Leggio; Maher Abou Hachem; Takane Katayama
        Science Advances, Aug. 2019, Peer-reviewed, Lead author
      • Sharing of human milk oligosaccharides degradants within bifidobacterial communities in faecal cultures supplemented with Bifidobacterium bifidum
        Aina Gotoh; Toshihiko Katoh; Mikiyasu Sakanaka; Yiwei Ling; Chihaya Yamada; Sadaki Asakuma; Tadasu Urashima; Yusuke Tomabechi; Ayako Katayama-Ikegami; Shin Kurihara; Kenji Yamamoto; Gaku Harata; Fang He; Junko Hirose; Motomitsu Kitaoka; Shujiro Okuda; Takane Katayama
        Scientific Reports, Dec. 2018, Peer-reviewed
      • Analysis of polyamine biosynthetic- and transport ability of human indigenous Bifidobacterium
        Yuta Sugiyama; Misaki Nara; Mikiyasu Sakanaka; Aya Kitakata; Shujiro Okuda; Shin Kurihara
        Bioscience, Biotechnology, and Biochemistry, Sep. 2018, Peer-reviewed
      • A Transposon Mutagenesis System forBifidobacterium longumsubsp.longumBased on an IS3Family Insertion Sequence, ISBlo11
        Mikiyasu Sakanaka; Shingo Nakakawaji; Shin Nakajima; Satoru Fukiya; Arisa Abe; Wataru Saburi; Haruhide Mori; Atsushi Yokota
        Applied and Environmental Microbiology, Jun. 2018, Peer-reviewed, Lead author
      • Functional analysis of arginine decarboxylase gene speA of Bacteroides dorei by markerless gene deletion
        Mikiyasu Sakanaka; Yuta Sugiyama; Misaki Nara; Aya Kitakata; Shin Kurihara
        FEMS Microbiology Letters, Feb. 2018, Peer-reviewed, Lead author
      • Comprehensive analysis of polyamine transport and biosynthesis in the dominant human gut bacteria: Potential presence of novel polyamine metabolism and transport genes
        Yuta Sugiyama; Misaki Nara; Mikiyasu Sakanaka; Aina Gotoh; Aya Kitakata; Shujiro Okuda; Shin Kurihara
        The International Journal of Biochemistry & Cell Biology, Dec. 2017, Peer-reviewed
      • Use of Gifu Anaerobic Medium for culturing 32 dominant species of human gut microbes and its evaluation based on short-chain fatty acids fermentation profiles
        Aina Gotoh; Misaki Nara; Yuta Sugiyama; Mikiyasu Sakanaka; Hiroyuki Yachi; Aya Kitakata; Akira Nakagawa; Hiromichi Minami; Shujiro Okuda; Toshihiko Katoh; Takane Katayama; Shin Kurihara
        Bioscience, Biotechnology, and Biochemistry, Oct. 2017, Peer-reviewed
      • Molecular Insight into Evolution of Symbiosis between Breast-Fed Infants and a Member of the Human Gut Microbiome Bifidobacterium longum
        Chihaya Yamada; Aina Gotoh; Mikiyasu Sakanaka; Mitchell Hattie; Keith A. Stubbs; Ayako Katayama-Ikegami; Junko Hirose; Shin Kurihara; Takatoshi Arakawa; Motomitsu Kitaoka; Shujiro Okuda; Takane Katayama; Shinya Fushinobu
        Cell Chemical Biology, Apr. 2017, Peer-reviewed
      • A Novel Putrescine Exporter SapBCDF ofEscherichia coli
        Yuta Sugiyama; Atsuo Nakamura; Mitsuharu Matsumoto; Ayaka Kanbe; Mikiyasu Sakanaka; Kyohei Higashi; Kazuei Igarashi; Takane Katayama; Hideyuki Suzuki; Shin Kurihara
        Journal of Biological Chemistry, Dec. 2016, Peer-reviewed
      • Carboxyspermidine decarboxylase of the prominent intestinal microbiota species Bacteroides thetaiotaomicron is required for spermidine biosynthesis and contributes to normal growth
        Mikiyasu Sakanaka; Yuta Sugiyama; Aya Kitakata; Takane Katayama; Shin Kurihara
        Amino Acids, Oct. 2016, Peer-reviewed, Lead author
      • Isolation and transposition properties of ISBlo11, an active insertion sequence belonging to the IS3 family, from Bifidobacterium longum 105-A
        Mikiyasu Sakanaka; Satoru Fukiya; Ryoko Kobayashi; Arisa Abe; Yosuke Hirayama; Yasunobu Kano; Atsushi Yokota
        FEMS Microbiology Letters, 01 Apr. 2015, Peer-reviewed, Lead author
      • Complete Genome Sequence of Bifidobacterium longum 105-A, a Strain with High Transformation Efficiency
        Y. Kanesaki; H. Masutani; M. Sakanaka; Y. Shiwa; T. Fujisawa; Y. Nakamura; A. Yokota; S. Fukiya; T. Suzuki; H. Yoshikawa
        Genome Announcements, Dec. 2014, Peer-reviewed, Lead author
      • Functional analysis of bifidobacterial promoters in Bifidobacterium longum and Escherichia coli using the α-galactosidase gene as a reporter
        Mikiyasu Sakanaka; Saki Tamai; Yosuke Hirayama; Ai Onodera; Hiroka Koguchi; Yasunobu Kano; Atsushi Yokota; Satoru Fukiya
        Journal of Bioscience and Bioengineering, Nov. 2014, Peer-reviewed, Lead author
      • Development of a Double-Crossover Markerless Gene Deletion System in Bifidobacterium longum: Functional Analysis of the α-Galactosidase Gene for Raffinose Assimilation
        Yosuke Hirayama; Mikiyasu Sakanaka; Hidenori Fukuma; Hiroki Murayama; Yasunobu Kano; Satoru Fukiya; Atsushi Yokota
        Applied and Environmental Microbiology, Jul. 2012, Peer-reviewed
      • Technological Advances in Bifidobacterial Molecular Genetics: Application to Functional Genomics and Medical Treatments
        Satoru FUKIYA; Yosuke HIRAYAMA; Mikiyasu SAKANAKA; Yasunobu KANO; Atsushi YOKOTA
        Bioscience of Microbiota, Food and Health, Apr. 2012, Peer-reviewed

      Misc.

      • 乳児期の腸内細菌叢とヒト母乳オリゴ糖:分子・菌体・菌叢レベルでの解析から見えてきたこと
        幹祥 阪中; 高嶺 片山
        Bulletin of Applied Glycoscience, 2022, Lead author, Corresponding author
      • Diverse strategies for utilizing fucosylated human milk oligosaccharides found in bifidobacteria
        Mikiyasu Sakanaka
        Glycoforum, 2022, Lead author, Last author, Corresponding author
      • 人乳成分を介した乳児期の腸内細菌叢形成およびヒトとの相互作用
        阪中幹祥; 片山高嶺
        FFIジャーナル, 2022, Lead author
      • ヒト母乳オリゴ糖に高く適応しているビフィズス菌種Bifidobacterium longum subsp. infantis
        阪中幹祥
        ミルクサイエンス, 2021, Lead author, Last author, Corresponding author
      • 諸外国で始まった乳児用調製粉乳への2′-フコシルラクトース添加—その科学的背景と現状—
        阪中幹祥; 片山高嶺
        化学と生物, 2020, Lead author
      • ヒト母乳オリゴ糖を介した乳児腸管におけるビフィズスフローラ形成機構
        後藤愛那; 加藤紀彦; 阪中幹祥; 片山高嶺
        腸内フローラの形成と疾患, 2018
      • Genetic manipulation and gene modification technologies in bifidobacteria
        Satoru Fukiya; Mikiyasu Sakanaka; Atsushi Yokota
        The bifidobacteria and related organisms: biology, taxonomy, applications, 2017
      • 母乳オリゴ糖によって支えられる腸内細菌と宿主の共生
        阪中幹祥
        生物工学会誌, 2017, Lead author, Last author, Corresponding author
      • 遺伝子変異導入法の確立により見えてきたビフィズス菌の腸管定着因子
        阪中幹祥; 吹谷智; 横田篤
        日本乳酸菌学会誌, 2013, Lead author

      Presentations

      • ビフィズス菌とヒトの共生を支える腸内代謝物
        阪中 幹祥
        日本微生物生態学会第36回浜松大会, Nov. 2023, Invited
      • ヒト乳児腸内に棲息するビフィズス菌の生理学的役割:母乳成分の代謝から分かってきたこと
        阪中幹祥
        第95回日本細菌学会総会, Mar. 2022, Invited
      • 母乳オリゴ糖利用戦略から見えてきたビフィズス菌と宿主の共進化の軌跡
        阪中幹祥
        酪農科学シンポジウム2021, Sep. 2021, Invited
      • 人乳成分の代謝から考えるビフィズス菌の適応進化戦略
        阪中幹祥
        ビフィズス菌研究会設立記念第一回シンポジウム, Mar. 2021, Invited
      • Adaptive evolution strategy of bifidobacteria found from the characterization of human milk oligosaccharide transporters
        Mikiyasu Sakanaka
        The 43rd Annual Meeting of the Molecular Biology Society of Japan, Dec. 2020, Invited
      • 乳児腸内で優勢なビフィズス菌の母乳オリゴ糖の資化戦略
        阪中幹祥
        第10回北陸合同バイオシンポジウム, Nov. 2017, Invited

      External funds: Kakenhi

      • Molecular mechanisms underlying the utilisation of oligosaccharides by infant gut-associated bacteria
        Grant-in-Aid for Early-Career Scientists
        Basic Section 38020:Applied microbiology-related
        Kyoto University
        Mikiyasu Sakanaka
        From 01 Apr. 2021, To 31 Mar. 2023, Project Closed
        腸内細菌;ビフィズス菌;オリゴ糖;分子機構
      • Sugar utilization strategy of bifidobacteria that supports the symbiosis with their hosts
        Grant-in-Aid for Early-Career Scientists
        Basic Section 38020:Applied microbiology-related
        Kyoto University;Ishikawa Prefectural University
        Mikiyasu Sakanaka
        From 01 Apr. 2018, To 31 Mar. 2021, Project Closed
        ビフィズス菌;母乳オリゴ糖;トランスポーター;遺伝子操作;共生;遺伝子破壊
      • シアロオリゴ糖代謝を起点とした腸内細菌と宿主の共生機構の解明
        Grant-in-Aid for Scientific Research (B)
        Basic Section 43030:Functional biochemistry-related
        Kyoto University
        阪中 幹祥
        From 01 Apr. 2023, To 31 Mar. 2026, Granted
        腸内細菌;ビフィズス菌;シアロオリゴ糖;シアル酸
      list
        Last Updated :2024/02/21

        Education

        Teaching subject(s)

        • From 01 Apr. 2023, To 31 Mar. 2024
          Experimental Practice in Biology I
          N420, Spring, Institute for Liberal Arts and Sciences, 2
        • From 01 Apr. 2022, To 31 Mar. 2023
          Experimental Practice in Biology I
          N420, Spring, Institute for Liberal Arts and Sciences, 2

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