Visualization of molecular mechanism of learning and memory
Overview of the research
When we learn and remember something, the efficacy of information transmission between neurons change in the brain. The information transmission occurs at a synapse, where a presynaptic neuron secretes transmitter molecule such as glutamate, and receptor proteins on the postsynaptic membrane capture transmitter and respond. Repeated use of a synapse increases the transmission efficacy for long-term, which is called long-term potentiation (LTP), and contributes to the formation of learning and memory. The increase in number of AMPA-type glutamate receptor (AMPAR) is a main molecular mechanism for LTP. However, when and how different types of AMPAR increase in the postsynaptic membrane has been unclear. Therefore, we address this issue by developing new experimental methods to visualize the change and movement of AMPAR around postsynaptic membrane using total internal reflection fluorescence microscopy.
Research Interests
Visualization of molecular mechanism of learning and memory
Synapse formation
Total internal reflection fluorescence microscopy
AMPA-type glutamate receptor
Live-cell imaging
Synaptic plasticity
Research Areas
Life sciences, Neuroscience - general
Life sciences, Neuroscience - general
Papers
Boltzmann Thermometry in Cr 3+ ‐Doped Ga 2 O 3 Polymorphs: The Structure Matters!
Frontiers in Cellular Neuroscience, 23 May 2018, Peer-reviewed
Detection and characterization of individual endocytosis of AMPA-type glutamate receptor around postsynaptic membrane
Shumpei Fujii; Hiromitsu Tanaka; Tomoo Hirano
GENES TO CELLS, Jun. 2017, Peer-reviewed
Live-cell imaging of receptors around postsynaptic membranes
Hiromitsu Tanaka; Shumpei Fujii; Tomoo Hirano
NATURE PROTOCOLS, Jan. 2014, Peer-reviewed
Visualization of Subunit-Specific Delivery of Glutamate Receptors to Postsynaptic Membrane during Hippocampal Long-Term Potentiation
Hiromitsu Tanaka; Tomoo Hirano
CELL REPORTS, Apr. 2012, Peer-reviewed
Misc.
脳・神経科学の基礎(脳化学1,数学者のための分子生物学入門-新しい数学を造ろう-)
平野 丈夫; 田中 洋光
物性研究, 2006
Visualization of the molecular mechanism of memory using new experimental methods
Hiromitsu Tanaka; Tomoo Hirano
Research Activities 2012, Kyoto University, Jun. 2012, Invited
Subunit-specific translocation of AMPA Receptors during hippocampal LTP
Hiromitsu Tanaka; Tomoo Hirano
NEUROSCIENCE RESEARCH, 2011
Presentations
Original experimental system to live-cell image neurotransmitter receptors and synaptic vesicle protein by TIRFM
Hiromitsu Tanaka
Utrecht University Seminar, 23 Mar. 2023, Invited
Visualization of the movement and location of neurotransmitter receptors and synaptic vesicle protein with TIRFM
Hiromitsu Tanaka
IINS seminar, 21 Mar. 2023, Invited
シナプス形成誘導技術を用いたシナプス小胞のリサイクリング機構の解明
田中洋光
第7回脳と心の研究会, 02 Sep. 2022
Fluorescent visualization of presynaptic endocytosed vesicles after exocytosis at individual hippocampal presynaptic active zones
Hiromitsu Tanaka; Tomoo Hirano
日本神経科学学会, 30 Jun. 2022
Visualization of uptake of synaptic vesicles at synaptic transmission
田中洋光
Brain and Mind Workshop 2021, 17 Feb. 2021
Total Internal Reflection Fluorescence Microscopy In Neuroscience
Hiromitsu Tanaka
The Francis Crick Institute seminar, 02 Mar. 2020
全反射顕微鏡を用いたAMPA型グルタミン酸受容体の動態イメージング
田中 洋光; 平野 丈夫
生理研研究会 (大阪), 01 Oct. 2019, Invited
シナプス小胞の開口放出及び再構築過程の可視化
田中 洋光
第4回脳と心の研究会(東京), 31 Aug. 2019, Invited
アミロイドベータによるサブユニット特異的なグルタミン酸受容体の動態異常
田中 洋光; 坂口 大輝; 平野 丈夫
第41回日本分子生物学会年会, 30 Nov. 2018
Subunit-specific effects on AMPA-type glutamate receptors caused by amyloid beta oligomers during hippocampal long-term potentiation
Hiromitsu Tanaka; Daiki Sakaguchi; Tomoo Hirano
Neurosciecne 2018 (SfN), San Diego (USA), Poster presentation (738.20), 07 Nov. 2018
全反射顕微鏡を用いたシナプス前終末とシナプス後膜研究
田中 洋光
第3回脳と心の研究会 (佐賀), 15 Sep. 2018, Invited
Subunit-specific effects of glutamate receptors caused by Abeta oligomers during long-term potentiation
Hiromitsu Tanaka
Brain Protein Aging and Dementia Control 2nd International Symposium, Poster presentation,, Nov. 2017
AMPA型グルタミン酸受容体の動態把握
田中 洋光
第2回脳と心の研究会 (京都), 27 Aug. 2017, Invited
シナプスにおける受容体動態の可視化法構築とその応用展開
田中 洋光
大阪市立大学セミナー (大阪), 12 May 2017, Invited
人工シナプス後膜の形成法開発と神経変性疾患研究への応用
田中 洋光
東北大学第6回神経内科リサーチセミナー (仙台), 10 May 2017, Invited
Imaging patgological effects of amyloid beta oligomers at synapses
Hiromitsu Tanaka
The UK-Japan Spring Neuroscience Symposium (Kyoto), Poster presentation (7), Mar. 2017
シナプス後膜における神経伝達物質受容体の動態変化と数的変化の定量解析
田中 洋光
定量生物学の第8回年会 (岡崎)、ポスター発表 (P59), Jan. 2017
Analyses of cell-surface amount, individual endo- and exocytosis of AMPA receptors, revealed suppression of exocytosis is important in hippocampal LTD
Shumpei Fujii; Hiromitsu Tanaka; Tomoo Hirano
Neuroscience 2016 (SfN), San Diego (USA), Poster presentation (223.14/N17), Nov. 2016
Dynamics of synaptic vesicle protein after single vesicle exocytosis at a hippocampal presynaptic active zone recorded by a novel live-cell imaging method
シナプス後膜内外におけるグルタミン酸受容体の個々のエキソサイトーシスとエンドサイトーシスの検出法開発 (New method to live-cell image individual exocytosis and endocytosis of glutamate receptors in postsynaptic and extrasynaptic membrane)
田中 洋光; 藤井 俊平; 平野 丈夫
日本細胞生物学会 (京都)、ポスター発表 (P1-9), Jun. 2016
Accurate estimation of AMPA receptor amount on the cell surface during hippocampal long-term depression (海馬長期抑圧時における膜表面AMPA受容体の量的変化の精確な推定)
Shumpei Fujii; Hiromitsu Tanaka; Tomoo Hirano
The 93rd Annual Meeting of the Physiological Society of Japan (Sapporo), Poster presentation (1P-043), Mar. 2016
Visualization of exocytosis and endocytosis around hippocampal presynaptic active zones by total internal reflection fluorescence microscopy (海馬シナプス前部におけるエキソサイトーシスとエンドサイトーシスの全反射顕微鏡による可視化)
Pathological effects of amyloid beta oligomers on synaptic plasticity
Hiromitsu Tanaka; Daiki Sakaguchi; Tomoo Hirano
Brain Protein Aging and Dementia Control 1st International Symposium (Nagoya), Oct. 2015
Visualization of Ca2+ dynamics and exocytosis at hippocampal presynaptic varicosities with total internal reflection fluorescent microscopy (海馬シナプス前部におけるCa2+濃度変化とエキソサイトーシスの全反射蛍光顕微鏡による可視化)