Basic Information

Professional Memberships

• From Oct. 2021, To Present

  The Molecular Biology Society of Japan
• From Oct. 2021, To Present

  The Japanese Society for Regenerative Medicine
• From Jun. 2021, To Present

  The Japanese Society for Genome Editing

Academic Degree

• 修士（工学）(名古屋大学)
• 博士（工学）(名古屋大学)

Research History

• From Apr. 2021, To Present

  Center for iPS Cell Research and Application, Kyoto University, Department of Clinical application, Hotta Lab, Project-specific Research Center Assistant professor
• From Jul. 2020, To Mar. 2021

  Kyoto University CiRA, Department of Clinical Application, Dr. Hotta's Lab, Postdoctral Researcher
• From Mar. 2020, To Jun. 2020

  Mayo Clinic, Department of Oncology, Research Associate
• From Mar. 2015, To Mar. 2020

  Mayo Clinic, Department of Oncology, Research Fellow

ID,URL

• ORCID ID

  0000-0001-7837-6102

• J-Global ID

  202001002866315727

• Researcher number

  90896416

researchmap URL

• https://researchmap.jp/yusuke.kojima29

Research

Research Interests

• Retrovirus
• Oligosaccharides
• Biopharmaceuticals
• Transgenic chickens
• Proteases
• Topoisomerase inhibitors
• PARP inhibitors
• Cancer drugs
• DNA replication stress
• DNA replication
• DNA-protein crosslink
• Cancer
• DNA repair
• DNA damage

Research Areas

• Life sciences, Virology, Retrovirus
• Manufacturing technology (mechanical, electrical/electronic, chemical engineering), Applied biofunctional and bioprocess engineering, Genetic engineering
• Life sciences, Tumor biology, DNA damage repair

Papers

• Dual CRISPR-Cas3 system for inducing multi-exon skipping in DMD patient-derived iPSCs

  Yuto Kita; Yuya Okuzaki; Youichi Naoe; Joseph Lee; Uikyu Bang; Natsumi Okawa; Akane Ichiki; Tatsuya Jonouchi; Hidetoshi Sakurai; Yusuke Kojima; Akitsu Hotta

  Stem Cell Reports, Aug. 2023, Peer-reviewed

  Access this paper :

  
• Regulatory mechanism of chicken lysozyme gene expression in oviducts examined using transgenic technology.

  Yusuke Kojima; Yuya Okuzaki; Ken-Ichi Nishijima; Shuichiro Moriwaki; Seiya Asai; Hidenori Kaneoka; Shinji Iijima

  Journal of bioscience and bioengineering, Apr. 2021, Peer-reviewed, Lead author

  
• Tandem Deubiquitination and Acetylation of SPRTN Promotes DNA-Protein Crosslink Repair and Protects against Aging

  Jinzhou Huang; Qin Zhou; Ming Gao; Somaira Nowsheen; Fei Zhao; Wootae Kim; Qian Zhu; Yusuke Kojima; Ping Yin; Yong Zhang; Guijie Guo; Xinyi Tu; Min Deng; Kuntian Luo; Bo Qin; Yuichi Machida; Zhenkun Lou

  Molecular Cell, Sep. 2020

  
• DNA-Protein Crosslinks from Environmental Exposure: Mechanisms of Formation and Repair.

  Yusuke Kojima; Yuichi J Machida

  Environmental and molecular mutagenesis, 23 Apr. 2020, Peer-reviewed, Lead author

  
• FAM111A protects replication forks from protein obstacles via its trypsin-like domain.

  Yusuke Kojima; Yuka Machida; Sowmiya Palani; Thomas R Caulfield; Evette S Radisky; Scott H Kaufmann; Yuichi J Machida

  Nature communications, 12 Mar. 2020, Peer-reviewed, Lead author

  
• Spartan deficiency causes accumulation of Topoisomerase 1 cleavage complexes and tumorigenesis.

  Reeja S Maskey; Karen S Flatten; Cynthia J Sieben; Kevin L Peterson; Darren J Baker; Hyun-Ja Nam; Myoung Shin Kim; Thomas C Smyrk; Yusuke Kojima; Yuka Machida; Annyoceli Santiago; Jan M van Deursen; Scott H Kaufmann; Yuichi J Machida

  Nucleic acids research, 05 May 2017, Peer-reviewed

  
• Analyses of chicken sialyltransferases related to O-glycosylation.

  Shunsuke Kidani; Hidenori Kaneoka; Yuya Okuzaki; Seiya Asai; Yusuke Kojima; Ken-Ichi Nishijima; Shinji Iijima

  Journal of bioscience and bioengineering, Oct. 2016, Peer-reviewed

  
• Analyses of chicken sialyltransferases related to N-glycosylation.

  Yusuke Kojima; Akifumi Mizutani; Yuya Okuzaki; Ken-Ichi Nishijima; Hidenori Kaneoka; Takako Sasamoto; Katsuhide Miyake; Shinji Iijima

  Journal of bioscience and bioengineering, Jun. 2015, Peer-reviewed, Lead author

  
• Galactosylation of human erythropoietin produced by chimeric chickens expressing galactosyltransferase.

  Yusuke Kojima; Jun Wakita; Yujin Inayoshi; Ryosuke Suzuki; Yuki Yamada; Hidenori Kaneoka; Ken-ichi Nishijima; Shinji Iijima

  Journal of bioscience and bioengineering, Jun. 2014, Peer-reviewed, Lead author

  
• Moloney murine leukemia virus integrase and reverse transcriptase interact with PML proteins.

  Yuuki Okino; Yujin Inayoshi; Yusuke Kojima; Shunsuke Kidani; Hidenori Kaneoka; Akiko Honkawa; Hiroshi Higuchi; Ken-ichi Nishijima; Katsuhide Miyake; Shinji Iijima

  Journal of biochemistry, Aug. 2012, Peer-reviewed

  

Misc.

• 3P-239 Oviduct-specific expression of therapeutic protein utilizing chicken lysozyme promoter

  Moriwaki Shuichiro; Kojima Yusuke; Okuzaki Yuya; Asai Seiya; Kaneoka Hidenori; Nishijima Ken-ichi; Iijima Shinji

  日本生物工学会大会講演要旨集, 2015

  
• 3P-241 Analyses of chicken sialyltransferases

  Kojima Yusuke; Kidani Shunsuke; Kaneoka Hidenori; Nishijima Ken-ichi; Iijima Shinji

  日本生物工学会大会講演要旨集, 2015

  
• 1P-043 Analysis of Chicken Sialyltransferase

  Okuzaki Yuya; Kojima Yusuke; Mizutani Akifumi; Nishijima Ken-ichi; Kaneoka Hidenori; Miyake Katsuhide; Iijima Shinji

  日本生物工学会大会講演要旨集, 2014

  

Presentations

• Genome Editing in iPS Cells -Applications and Methods-

  Yusuke Kojima; Akitsu Hotta

  The 9th Annual Meeting for the Japanese Society for Genome Editing, 17 Jun. 2024, Invited

  
• HLA Genome Editing in iPS Cells by RNA-besed CRISPR-Cas3 Method

  Yusuke Kojima; Chika Kuwabara; Yuya Okuzaki; Akitsu Hotta

  第２３回日本再生医療学会総会, 23 Mar. 2024

  
• RNAベースCRISPR-Cas3法を用いたiPS細胞におけるゲノム編集

  小島佑介; 桑原千佳; 奥嵜雄也; 堀田秋津

  第４６回日本分子生物学会年会, 08 Dec. 2023

  
• FAM111A protects replication forks from protein obstacles via its trypsin-like domain

  Yusuke Kojima; Yuka Machida; Sowmiya Palani; Thomas R. Caulfield; Evette S. Radisky; Scott H. Kaufmann; Yuichi J. Machida

  Gordon Research Conference, 03 Mar. 2020

  
• FAM111A protects replication forks from protein obstacles via its trypsin-like domain

  Yusuke Kojima; Yuka Machida; Sowmiya Palani; Thomas R. Caulfield; Evette S. Radisky; Scott H. Kaufmann; Yuichi J. Machida

  Gordon Research Seminar, 29 Feb. 2020

  
• Repair of DNA-Protein Crosslinks and Its Implication for Cancer Therapies

  Yusuke Kojima

  Visiting Faculty Seminar, Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, 21 Feb. 2020, Invited

  
• ニワトリリゾチームプロモーターを用いた卵白特異的有用タンパク質生産

  森脇脩一郎; 小島佑介; 奥嵜雄也; 淺井聖也; 金岡英徳; 西島謙一; 飯島信司

  日本生物工学会, 28 Oct. 2015

  
• ニワトリシアル酸転移酵素の解析

  小島佑介; 木溪俊介; 金岡英徳; 西島謙一; 飯島信司

  日本生物工学会, 28 Oct. 2015

  
• ニワトリリゾチームプロモーターを利用した鶏卵卵白中への有用タンパク質生産

  小島佑介; 森脇脩一郎; 金岡英徳; 西島謙一; 飯島信司

  日本分子生物学会, 27 Nov. 2014

  
• Analysis of chicken sialyltransferases for pharmaceutical protein production in egg white by transgenic chickens

  Yusuke Kojima

  Japanese Association for Animal Cell Technology

  
• ニワトリシアル酸転移酵素の解析

  奥嵜雄也; 小島佑介; 水谷昭文; 西島謙一; 金岡英徳; 三宅克英; 飯島信司

  日本生物工学会, 09 Sep. 2014

  
• 遺伝子導入ニワトリを用いたヒトエリスロポエチンへのガラクトース付加

  小島佑介

  日本生物工学会中部支部, 02 Aug. 2013

  
• 遺伝子導入ニワトリを用いたヒトエリスロポエチンへのガラクトース付加

  小島佑介

  日本動物細胞工学会, 18 Jul. 2013

  
• キメラニワトリによるヒトエリスロポエチンへのガラクトース付加

  小島佑介; 脇田潤; 小島康裕; 山田裕貴; 西島謙一; 飯島信司

  日本農芸化学会

  

Books and Other Publications

• 実験医学 特集 動き始めたゲノム編集の医療応用

  パン イギュ; 小島 佑介; 堀田 秋津, Contributor, in vivo ゲノム編集の鍵を握るデリバリー技術

  羊土社, Apr. 2024

  Find Kyoto University's collection of books :

  
• Cell cycle machinery and its alterations in pancreatic cancer

  Yusuke Kojima; Reeja S. Maskey; Yuichi J. Machida, Joint work, First author

  Springer New York, Apr. 2018

  

External funds: Kakenhi

• タンパク質障害物を利用したCRISPR-Cas3による遺伝子欠損領域の制御

  Grant-in-Aid for Early-Career Scientists

  Basic Section 48040:Medical biochemistry-related

  Kyoto University

  小島 佑介

  From 01 Apr. 2021, To 31 Mar. 2024, Granted

  ゲノム編集;CRISPR-Cas;遺伝子ノックアウト;タンパク質障害物;遺伝子疾患;遺伝子治療

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K15361/
• 近傍集積因子解析よるCRISPR-Cas3ゲノム欠失誘導機構の解明

  Grant-in-Aid for Scientific Research (B)

  Basic Section 43050:Genome biology-related

  Kyoto University

  堀田 秋津

  From 01 Apr. 2022, To 31 Mar. 2025, Granted

  CRISPR-Cas3;ゲノム編集;DNA損傷修復

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H02601/
• CRISPR-Cas3ゲノム編集に影響を与える因子に関する研究

  Grant-in-Aid for Scientific Research (C)

  Basic Section 48040:Medical biochemistry-related

  Kyoto University

  小島 佑介

  From 01 Apr. 2024, To 31 Mar. 2027, Granted

  ゲノム編集;CRISPR-Cas3

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K10064/
• 近傍集積因子解析よるCRISPR-Cas3ゲノム欠失誘導機構の解明

  Grant-in-Aid for Scientific Research (B)

  Basic Section 43050:Genome biology-related

  Kyoto University

  堀田 秋津

  From 01 Apr. 2022, To 31 Mar. 2025, Granted

  ゲノム編集;CRISPR-Cas3;DNA損傷修復

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K23864/

Academic, Social Contribution

Committee Memberships

• From Jun. 2022, To Jun. 2022

  7th Annual Meeting Committee Member, Japanese Society for Genome Editing