Basic Information

Academic Degree

• 修士（食品栄養科学）(静岡県立大学)
• 博士（医学）(京都府立医科大学)

ID,URL

• J-Global ID

  200901034420320345

researchmap URL

• https://researchmap.jp/read0103912

Research

Research Interests

• GPCR
• structural biology

Research Areas

• Life sciences, Immunology
• Life sciences, Molecular biology
• Life sciences, Structural biochemistry

Papers

• Dual 5-HT_2A and 5-HT_2C Receptor Inverse Agonist That Affords In Vivo Antipsychotic Efficacy with Minimal hERG Inhibition for the Treatment of Dementia-Related Psychosis

  Takuya Oguma; Kohei Jino; Kenji Nakahara; Hidetsugu Asada; Kouki Fuchino; Kotaro Nagatani; Kensuke Kouki; Ryuji Okamoto; Nozomi Takai; Ken Koda; Sayaka Fujita; Yusuke Sekiguchi; Kazuya Yasuo; Kei Mayumi; Ayane Abe; Masaaki Imono; Naotaka Horiguchi; So Iwata; Ken-ichi Kusakabe

  Journal of Medicinal Chemistry, 13 Aug. 2024

  
• In Vitro Pharmacological Profile of KW-6356, a Novel Adenosine A2A Receptor Antagonist/Inverse Agonist.

  Yutaro Ohno; Michihiko Suzuki; Hidetsugu Asada; Tomoyuki Kanda; Mayumi Saki; Hikaru Miyagi; Mai Yasunaga; Chiyo Suno; So Iwata; Jun-Ichi Saito; Shinichi Uchida

  Molecular pharmacology, Jun. 2023

  
• Crystal structure reveals the binding mode and selectivity of a photoswitchable ligand for the adenosine A2A receptor.

  Tsuyoshi Araya; Yuya Matsuba; Harufumi Suzuki; Tomohiro Doura; Nipawan Nuemket; Eriko Nango; Masaki Yamamoto; Dohyun Im; Hidetsugu Asada; Shigeki Kiyonaka; So Iwata

  Biochemical and biophysical research communications, 05 Feb. 2024

  
• Structural insights into the agonists binding and receptor selectivity of human histamine H4 receptor.

  Dohyun Im; Jun-Ichi Kishikawa; Yuki Shiimura; Hiromi Hisano; Akane Ito; Yoko Fujita-Fujiharu; Yukihiko Sugita; Takeshi Noda; Takayuki Kato; Hidetsugu Asada; So Iwata

  Nature communications, 20 Oct. 2023

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• Towards the crystal structure determination of muscarinic acetylcholine receptors

  Ryoji Suno; Hidetsugu Asada; Takuya Kobayashi

  Neuromethods, 2016

  
• Molecular basis for anti-insomnia drug design from structure of lemborexant-bound orexin 2 receptor.

  Hidetsugu Asada; Dohyun Im; Yunhon Hotta; Satoshi Yasuda; Takeshi Murata; Ryoji Suno; So Iwata

  Structure (London, England : 1993), 01 Dec. 2022

  
• 中分子創薬研究のフロンティア 内分泌ペプチド創薬の新時代を切り拓く X線結晶構造解析を基盤とした活性型グレリン認識機構の解明

  椎村 祐樹; 堀田 彰一朗; 浅田 秀基; 平田 邦夫; 小林 拓也; 岩田 想; 児島 将康

  日本薬学会年会要旨集, Mar. 2019, Peer-reviewed

  
• グレリン受容体の立体構造を認識するモノクローナル抗体作製の試み

  椎村 祐樹; 堀田 彰一朗; 浅田 秀基; 平田 邦生; 小林 拓也; 岩田 想; 児島 将康

  日本内分泌学会雑誌, Dec. 2018, Peer-reviewed

  
• Endogenous agonist-bound S1PR3 structure reveals determinants of G protein-subtype bias.

  Shintaro Maeda; Yuki Shiimura; Hidetsugu Asada; Kunio Hirata; Fangjia Luo; Eriko Nango; Nobuo Tanaka; Masayasu Toyomoto; Asuka Inoue; Junken Aoki; So Iwata; Masatoshi Hagiwara

  Science advances, Jun. 2021

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• Structure of the dopamine D2 receptor in complex with the antipsychotic drug spiperone

  Dohyun Im; Asuka Inoue; Takaaki Fujiwara; Takanori Nakane; Yasuaki Yamanaka; Tomoko Uemura; Chihiro Mori; Yuki Shiimura; Kanako Terakado Kimura; Hidetsugu Asada; Norimichi Nomura; Tomoyuki Tanaka; Ayumi Yamashita; Eriko Nango; Kensuke Tono; Francois Marie Ngako Kadji; Junken Aoki; So Iwata; Tatsuro Shimamura

  Nature Communications, Dec. 2020

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• Structure of an antagonist-bound ghrelin receptor reveals possible ghrelin recognition mode

  Yuki Shiimura; Shoichiro Horita; Akie Hamamoto; Hidetsugu Asada; Kunio Hirata; Misuzu Tanaka; Kenji Mori; Tomoko Uemura; Takuya Kobayashi; So Iwata; Masayasu Kojima

  Nature Communications, Dec. 2020, Peer-reviewed

  
• The discovery of a new antibody for BRIL-fused GPCR structure determination.

  Hikaru Miyagi; Hidetsugu Asada; Michihiko Suzuki; Yuichi Takahashi; Mai Yasunaga; Chiyo Suno; So Iwata; Jun-Ichi Saito

  Scientific reports, 15 Jul. 2020, Peer-reviewed

  
• The Crystal Structure of Angiotensin II Type 2 Receptor with Endogenous Peptide Hormone

  Hidetsugu Asada; Asuka Inoue; Francois Marie Ngako Kadji; Kunio Hirata; Yuki Shiimura; Dohyun Im; Tatsuro Shimamura; Norimichi Nomura; Hiroko Iwanari; Takao Hamakubo; Osamu Kusano-Arai; Hiromi Hisano; Tomoko Uemura; Chiyo Suno; Junken Aoki; So Iwata

  Structure, Dec. 2019, Peer-reviewed

  
• Structures of the 5-HT 2A receptor in complex with the antipsychotics risperidone and zotepine

  Kanako Terakado Kimura; Hidetsugu Asada; Asuka Inoue; Francois Marie Ngako Kadji; Dohyun Im; Chihiro Mori; Takatoshi Arakawa; Kunio Hirata; Yayoi Nomura; Norimichi Nomura; Junken Aoki; So Iwata; Tatsuro Shimamura

  Nature Structural and Molecular Biology, 01 Feb. 2019, Peer-reviewed

  
• Ligand binding to human prostaglandin E receptor EP4 at the lipid-bilayer interface.

  Yosuke Toyoda; Kazushi Morimoto; Ryoji Suno; Shoichiro Horita; Keitaro Yamashita; Kunio Hirata; Yusuke Sekiguchi; Satoshi Yasuda; Mitsunori Shiroishi; Tomoko Shimizu; Yuji Urushibata; Yuta Kajiwara; Tomoaki Inazumi; Yunhon Hotta; Hidetsugu Asada; Takanori Nakane; Yuki Shiimura; Tomoya Nakagita; Kyoshiro Tsuge; Suguru Yoshida; Tomoko Kuribara; Takamitsu Hosoya; Yukihiko Sugimoto; Norimichi Nomura; Miwa Sato; Takatsugu Hirokawa; Masahiro Kinoshita; Takeshi Murata; Kiyoshi Takayama; Masaki Yamamoto; Shuh Narumiya; So Iwata; Takuya Kobayashi

  Nature chemical biology, Jan. 2019, Peer-reviewed

  
• Crystal structure of the human angiotensin II type 2 receptor bound to an angiotensin II analog.

  Asada H; Horita S; Hirata K; Shiroishi M; Shiimura Y; Iwanari H; Hamakubo T; Shimamura T; Nomura N; Kusano-Arai O; Uemura T; Suno C; Kobayashi T; Iwata S

  Nature structural & molecular biology, Jul. 2018, Peer-reviewed

  
• グレリン受容体の結晶構造解析による活性型グレリン認識機構の解明

  椎村 祐樹; 堀田 彰一朗; 浅田 秀基; 平田 邦生; 寿野 良二; 小林 拓也; 岩田 想; 児島 将康

  日本内分泌学会雑誌, Apr. 2018, Peer-reviewed

  
• Proteoliposome-based Selection of a Recombinant Antibody Fragment Against the Human M2 Muscarinic Acetylcholine Receptor

  Suharni; Yayoi Nomura; Takatoshi Arakawa; Tomoya Hino; Hitomi Abe; Yoshiko Nakada-Nakura; Yumi Sato; Hiroko Iwanari; Mitsunori Shiroishi; Hidetsugu Asada; Tatsuro Shimamura; Takeshi Murata; Takuya Kobayashi; Takao Hamakubo; So Iwata; Norimichi Nomura

  Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, Dec. 2014, Peer-reviewed

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• Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae

  Mitsunori Shiroishi; Hirokazu Tsujimoto; Hisayoshi Makyio; Hidetsugu Asada; Takami Yurugi-Kobayashi; Tatsuro Shimamura; Takeshi Murata; Norimichi Nomura; Tatsuya Haga; So Iwata; Takuya Kobayashi

  MICROBIAL CELL FACTORIES, Jun. 2012, Peer-reviewed

  
• Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist

  Kazuko Haga; Andrew C. Kruse; Hidetsugu Asada; Takami Yurugi-Kobayashi; Mitsunori Shiroishi; Cheng Zhang; William I. Weis; Tetsuji Okada; Brian K. Kobilka; Tatsuya Haga; Takuya Kobayashi

  NATURE, Feb. 2012, Peer-reviewed

  
• Cloning, expression and purification of the anion exchanger 1 homologue from the basidiomycete Phanerochaete chrysosporium

  Natsuko Tokuda; Kiyohiko Igarashi; Tatsuro Shimamura; Takami Yurugi-Kobayashi; Mitsunori Shiroishi; Keisuke Ito; Taishi Sugawara; Hidetsugu Asada; Takeshi Murata; Norimichi Nomura; So Iwata; Takuya Kobayashi

  PROTEIN EXPRESSION AND PURIFICATION, Sep. 2011, Peer-reviewed

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• Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis

  Hidetsugu Asada; Tomoko Uemura; Takami Yurugi-Kobayashi; Mitsunori Shiroishi; Tatsuro Shimamura; Hirokazu Tsujimoto; Keisuke Ito; Taishi Sugawara; Takanori Nakane; Norimichi Nomura; Takeshi Murata; Tatsuya Haga; So Iwata; Takuya Kobayashi

  MICROBIAL CELL FACTORIES, Apr. 2011, Peer-reviewed

  
• Towards structure determination for G protein-coupled receptor

  Takuya Kobayashi; Mitsunori Shiroishi; Tatsuro Shimamura; Takami Yurugi; Hidetsugu Asada; Tomoya Hino; Takatoshi Arakawa; Takeshi Murata; Norimichi Nomura; So Iwata

  JOURNAL OF PHARMACOLOGICAL SCIENCES, 2011, Peer-reviewed

  
• Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion: case of a nonglycosylated mutant of miraculin, a taste-modifying protein

  Keisuke Ito; Taishi Sugawara; Ayako Koizumi; Ken-ichiro Nakajima; Akiko Shimizu-Ibuka; Mitsunori Shiroishi; Hidetsugu Asada; Takami Yurugi-Kobayashi; Tatsuro Shimamura; Tomiko Asakura; Takumi Misaka; So Iwata; Takuya Kobayashi; Keiko Abe

  BIOTECHNOLOGY LETTERS, Jan. 2011, Peer-reviewed

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• Bulky high-mannose-type N-glycan blocks the taste-modifying activity of miraculin

  Keisuke Ito; Taishi Sugawara; Ayako Koizumi; Ken-ichiro Nakajima; Akiko Shimizu-Ibuka; Mitsunori Shiroishi; Hidetsugu Asada; Takami Yurugi-Kobayashi; Tatsuro Shimamura; Tomiko Asakura; Katsuyoshi Masuda; Masaji Ishiguro; Takumi Misaka; So Iwata; Takuya Kobayashi; Keiko Abe

  BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, Sep. 2010, Peer-reviewed

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• Osteoblasts derived from osteophytes produce interleukin-6, interleukin-8, and matrix metalloproteinase-13 in osteoarthritis

  Kei Sakao; Kenji A. Takahashi; Yuji Arai; Masazumi Saito; Kuniaki Honjo; Nobuyuki Hiraoka; Hidetsugu Asada; Masaharu Shin-Ya; Jiro Imanishi; Osam Mazda; Toshikazu Kubo

  JOURNAL OF BONE AND MINERAL METABOLISM, Jul. 2009, Peer-reviewed

  
• Fluorescence-based optimization of human bitter taste receptor expression in Saccharomyces cerevisiae

  Taishi Sugawara; Keisuke Ito; Mitsunori Shiroishi; Natsuko Tokuda; Hidetsugu Asada; Takami Yurugi-Kobayashi; Tatsuro Shimamura; Takumi Misaka; Norimichi Nomura; Takeshi Murata; Keiko Abe; So Iwata; Takuya Kobayashi

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, May 2009, Peer-reviewed

  
• Comparison of functional non-glycosylated GPCRs expression in Pichia pastoris

  Takami Yurugi-Kobayashi; Hidetsugu Asada; Mitsunori Shiroishi; Tatsuro Shimamura; Saeko Funamoto; Naoko Katsuta; Keisuke Ito; Taishi Sugawara; Natsuko Tokuda; Hirokazu Tsujimoto; Takeshi Murata; Norimichi Nomura; Kazuko Haga; Tatsuya Haga; So Iwata; Takuya Kobayashi

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Mar. 2009, Peer-reviewed

  
• Pleiotrophic functions of Epstein-Barr virus nuclear antigen-1 (EBNA-1) and oriP differentially contribute to the efficacy of transfection/expression of exogenous gene in mammalian cells

  Tsunao Kishida; Hidetsugu Asada; Koji Kubo; Yuko T. Sato; Masaharu Shin-Ya; Jiro Imanishi; Kenichi Yoshikawa; Osam Mazda

  JOURNAL OF BIOTECHNOLOGY, Jan. 2008, Peer-reviewed

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• IL-21 induces inhibitor of differentiation 2 and leads to complete abrogation of anaphylaxis in mice

  Tsunao Kishida; Yayoi Hiromura; Masaharu Shin-Ya; Hidetsugu Asada; Hiroko Kuriyama; Manabu Sugai; Akira Shimizu; Yoshifumi Yokota; Takemitsu Hama; Jiro Imanishi; Yasuo Hisa; Osam Mazda

  JOURNAL OF IMMUNOLOGY, Dec. 2007, Peer-reviewed

  
• Soluble TRAIL gene and actinomycin D synergistically suppressed multiple metastasis of TRAIL-resistant colon cancer in the liver

  Michiaki Ishii; Masaki Iwai; Yoshinori Harada; Tsunao Kishida; Hidetsugu Asada; Masaharu Shin-Ya; Yoshito Itoh; Jiro Imanishi; Takeshi Okanoue; Osam Mazda

  CANCER LETTERS, Jan. 2007, Peer-reviewed

  
• Combination vaccine of dendritic cells (DCs) and T cells effectively suppressed preestablished malignant melanoma in mice

  Hidetsugu Asada; Tsunao Kishida; Hideyo Hirai; Masaharu Shin-Ya; Jiro Imanishi; Minoru Takeuchi; Osam Mazda

  CANCER LETTERS, Aug. 2006, Peer-reviewed

  
• Transthoracic direct current shock facilitates intramyocardial transfection of naked plasmid DNA infused via coronary vessels in canines

  Y Iida; Y Oda; S Nakamori; S Tsunoda; T Kishida; S Gojo; M Shin-Ya; H Asada; J Imanishi; T Yoshikawa; H Matsubara; O Mazda

  GENE THERAPY, Jun. 2006, Peer-reviewed

  
• Interleukin-21 triggers both cellular and humoral immune responses leading to therapeutic antitumor effects against head and neck squamous cell carcinoma

  H Nakano; T Kishida; H Asada; M Shin-Ya; T Shinomiya; J Imanishi; T Shimada; S Nakai; M Takeuchi; Y Hisa; O Mazda

  JOURNAL OF GENE MEDICINE, Jan. 2006, Peer-reviewed

  
• The effect of apoptosis signal-regulating kinase 1 gene transfer on rat collagen induced arthritis

  R Terauchi; Y Arai; KA Takahashi; A Inoue; H Tonomura; H Asada; T Kishida; J Imanishi; O Mazda; Kitajima, I; T Kubo

  JOURNAL OF RHEUMATOLOGY, Dec. 2005, Peer-reviewed

  
• Electro-transfer of small interfering RNA ameliorated arthritis in rats

  A Inoue; KA Takahashi; O Mazda; R Terauchi; Y Arai; T Kishida; M Shin-Ya; H Asada; T Morihara; H Tonomura; S Ohashi; Y Kajikawa; Y Kawahito; J Imanishi; M Kawata; T Kubo

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Oct. 2005, Peer-reviewed

  
• Sonoporation using microbubble BR14 promotes pDNA/siRNA transduction to murine heart

  S Tsunoda; O Mazda; Y Oda; Y Iida; S Akabame; T Kishida; SY Masaharu; H Asada; S Gojo; J Imanishi; H Matsubara; T Yoshikawa

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Oct. 2005, Peer-reviewed

  
• Intracellular interferon triggers Jak/Stat signaling cascade and induces p53-dependent antiviral protection

  M Shin-Ya; H Hirai; E Satoh; T Kishida; H Asada; F Aoki; M Tsukamoto; J Imanishi; O Mazda

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Apr. 2005, Peer-reviewed

  
• Cytokine genetic adjuvant facilitates prophylactic intravascular DNA vaccine against acute and latent herpes simplex virus infection in mice

  FD Cui; H Asada; ML Jin; T Kishida; M Shin-Ya; T Nakaya; M Kita; M Ishii; M Iwai; T Okanoue; J Imanishi; O Mazda

  GENE THERAPY, Jan. 2005, Peer-reviewed

  
• Interleukin-12 genetic administration suppressed metastatic liver tumor unsusceptible to CTL

  Y Itokawa; O Mazda; Y Ueda; T Kishida; H Asada; FD Cui; N Fuji; H Fujiwara; M Shin-Ya; K Yasutomi; J Imanishi; H Yamagishi

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Feb. 2004, Peer-reviewed

  
• Sequence-specific gene silencing in murine muscle induced by etectroporation-mediated transfer of short interfering RNA

  T Kishida; H Asada; S Gojo; S Ohashi; M Shin-Ya; K Yasutomi; R Terauchi; KA Takahashi; T Kubo; J Imanishi; O Mazda

  JOURNAL OF GENE MEDICINE, Jan. 2004, Peer-reviewed

  
• Intravascular naked DNA vaccine encoding glycoprotein B induces protective humoral and cellular immunity against herpes simplex virus type 1 infection in mice

  FD Cui; H Asada; T Kishida; Y Itokawa; T Nakaya; Y Ueda; H Yamagishi; S Gojo; M Kita; J Imanishi; O Mazda

  GENE THERAPY, Dec. 2003, Peer-reviewed

  
• Electrochemo-gene therapy of cancer: Intratumoral delivery of interleukin-12 gene and bleomycin synergistically induced therapeutic immunity and suppressed subcutaneous and metastatic melanomas in mice

  T Kishida; H Asada; Y Itokawa; K Yasutomi; M Shin-ya; S Gojo; F Cui; Y Ueda; H Yamagishi; J Imanishi; O Mazda

  MOLECULAR THERAPY, Nov. 2003, Peer-reviewed

  
• Intravascular insulin gene delivery as potential therapeutic intervention in diabetes mellitus

  K Yasutomi; Y Itokawa; H Asada; T Kishida; FD Cui; S Ohashi; S Gojo; Y Ueda; T Kubo; H Yamagishi; J Imanishi; T Takeuchi; O Mazda

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Oct. 2003, Peer-reviewed

  
• Interleukin (IL)-21 and IL-15 genetic transfer synergistically augments therapeutic antitumor immunity and promotes regression of metastatic lymphoma

  T Kishida; H Asada; Y Itokawa; FD Cui; M Shin-Ya; S Gojo; K Yasutomi; Y Ueda; H Yamagishi; J Imanishi; O Mazda

  MOLECULAR THERAPY, Oct. 2003, Peer-reviewed

  
• Nonviral genetic transfer of Fas ligand induced significant growth suppression and apoptotic tumor cell death in prostate cancer in vivo (vol 10, pg 434, 2003)

  H Nakanishi; O Mazda; E Satoh; H Asada; H Morioka; T Kishida; M Nakao; Y Mizutani; A Kawauchi; M Kita; J Imanishi; T Miki

  GENE THERAPY, Oct. 2003, Peer-reviewed

  
• Nonviral genetic transfer of Fas ligand induced significant growth suppression and apoptotic tumor cell death in prostate cancer in vivo

  H Nakanishi; O Mazda; E Satoh; H Asada; H Morioka; T Kishida; M Nakao; Y Mizutani; A Kawauchi; M Kita; J Imanishi; T Miki

  GENE THERAPY, Mar. 2003, Peer-reviewed

  
• Significant antitumor effects obtained by autologous tumor cell vaccine engineered to secrete interleukin (IL)-12 and IL-18 by means of the EBV/lipoplex

  H Asada; T Kishida; H Hirai; E Satoh; S Ohashi; M Takeuchi; T Kubo; M Kita; Y Iwakura; J Imanishi; O Mazda

  MOLECULAR THERAPY, May 2002, Peer-reviewed

  
• Successful genetic transduction in vivo into synovium by means of electroporation

  S Ohashi; T Kubo; T Kishida; T Ikeda; K Takahashi; Y Arai; R Terauchi; H Asada; J Imanishi; O Mazda

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, May 2002, Peer-reviewed

  
• Highly efficient gene transfer into murine liver achieved by intravenous administration of naked Epstein-Barr virus (EBV)-based plasmid vectors

  FD Cui; T Kishida; S Ohashi; H Asada; K Yasutomi; E Satoh; T Kubo; S Fushiki; J Imanishi; O Mazda

  GENE THERAPY, Oct. 2001, Peer-reviewed

  
• In vivo electroporation-mediated transfer of interleukin-12 and interleukin-18 genes induces significant antitumor effects against melanoma in mice

  T Kishida; H Asada; E Satoh; S Tanaka; M Shinya; H Hirai; M Iwai; H Tahara; J Imanishi; O Mazda

  GENE THERAPY, Aug. 2001, Peer-reviewed

  
• Inhibition of lung natural killer cell activity by smoking: The role of alveolar macrophages

  M Takeuchi; S Nagai; A Nakajima; M Shinya; C Tsukano; H Asada; K Yoshikawa; M Yoshimura; T Izumi

  RESPIRATION, May 2001, Peer-reviewed

  

Misc.

• 構造情報を基づいたグレリン受容体アゴニストのシグナル活性の比較

  椎村 祐樹; イム・ドヒョン; 谷猪 遼介; 木瀬 亮次; 松井 一真; 浅田 秀基; 岩田 想; 児島 将康; 増保 生郎

  日本内分泌学会雑誌, Apr. 2024

  
• アゴニストと結合したグレリン受容体の構造に関する洞察(Insights into the Structure of the Agonist-bound Ghrelin Receptor)

  椎村 祐樹; イム・ドヒョン; 谷猪 遼介; 木瀬 亮次; 浅田 秀基; 岩田 想; 増保 生郎; 児島 将康

  日本内分泌学会雑誌, May 2023

  
• アゴニストと結合したグレリン受容体の立体構造

  椎村 祐樹; イム・ドヒョン; 浅田 秀基; 岩田 想; 児島 将康

  日本内分泌学会雑誌, Mar. 2023

  
• スフィンゴシン1-リン酸受容体の構造解析から明らかとなりつつある受容体活性化機構とシグナル伝達

  前田 信太郎; 浅田 秀基

  生化学, Oct. 2022

  
• グレリン受容体の立体構造解析

  椎村 祐樹; 浅田 秀基; 岩田 想; 児島 将康

  日本内分泌学会雑誌, Mar. 2022

  
• ニューノーマル時代に向けたステロイドホルモンと疾患研究の新展開 アンジオテンシンII受容体の立体構造と血圧調節メカニズム

  浅田 秀基; 岩田 想

  日本生化学会大会プログラム・講演要旨集, Nov. 2021

  
• スフィンゴシン-1-リン酸受容体におけるバイアスドアゴニズム機構

  前田 信太郎; 椎村 祐樹; 浅田 秀基; 平田 邦生; Luo Fangjia; 南後 恵理子; 井上 飛鳥; 青木 淳賢; 岩田 想; 萩原 正敏

  日本生化学会大会プログラム・講演要旨集, Nov. 2021

  
• 【イメージング時代の構造生命科学 細胞の動態、膜のないオルガネラ、分子の構造変化をトランススケールに観る】(第2章)構造生命科学からトランススケール・イメージングによる細胞動態学へ トランススケールな解析が待たれる生命科学の未解決課題 抗体フラグメントを用いたGPCR構造生命研究

  岩田 想; 浅田 秀基

  実験医学, Mar. 2020

  
• 生理活性物質アンジオテンシンIIの結合によるアンジオテンシンII受容体活性化機構の構造基盤

  浅田 秀基; 井上 飛鳥; Francois Kadji M.N.; 平田 邦生; 椎村 祐樹; Dohyun Im; 青木 淳賢; 岩田 想

  日本生化学会大会プログラム・講演要旨集, Sep. 2019

  
• 中分子創薬研究のフロンティア 内分泌ペプチド創薬の新時代を切り拓く X線結晶構造解析を基盤とした活性型グレリン認識機構の解明

  椎村 祐樹; 堀田 彰一朗; 浅田 秀基; 平田 邦夫; 小林 拓也; 岩田 想; 児島 将康

  日本薬学会年会要旨集, Mar. 2019

  
• ヒトアンジオテンシンIIタイプ2受容体(AT2R)の立体構造解析へ向けた検討

  浅田 秀基; 平田 邦生; 白石 充典; 岩成 宏子; 浜窪 隆雄; 小林 拓也; 岩田 想

  日本生化学会大会プログラム・講演要旨集, Sep. 2016

  
• ヒスタミンH1受容体の結晶構造解析

  白石 充典; 島村 達郎; 辻本 浩一; 浅田 秀基; 小林 貴美[万木]; 小林 拓也; 岩田 想

  生化学, Oct. 2012

  
• M2ムスカリン受容体のX線結晶構造解析

  万木 貴美[小林]; 浅田 秀基; 芳賀 和子; Kruse Andrew; 中根 崇智; 岡田 哲二; Zhang Cheng; Weis William; Kobilka Tong Sun; Kobilka Brian; 芳賀 達也; 小林 拓也

  日本生化学会大会プログラム・講演要旨集, Sep. 2011

  
• ムスカリン性アセチルコリン受容体M2サブタイプの結晶構造(Crystal structure of the human muscarinic acetylcholine receptor M2 subtype)

  芳賀 達也; 芳賀 和子; Kruse Andrew G.; 浅田 秀基; 小林 貴美[万木]; 中根 崇智; Kobilka Tong Sun; Zhang Cheng; Weis William I.; 岡田 哲二; Kobilka Brian; 小林 拓也

  日本生化学会大会プログラム・講演要旨集, Sep. 2011

  
• GPCRの構造研究に向けた安定化改変体の作製・評価系の確立

  白石 充典; 島村 達郎; 辻本 浩一; 浅田 秀基; 小林 貴美[万木]; 小林 拓也; 岩田 想

  日本生化学会大会プログラム・講演要旨集, Sep. 2011

  
• アンジオテンシンタイプ2受容体の発現・精製から結晶構造解析へ向けて

  浅田 秀基; 小林 貴美; 白石 充典; 島村 達郎; 植村 智子; 辻本 浩一; 中根 崇智; 岩田 想; 小林 拓也

  日本生化学会大会プログラム・講演要旨集, Sep. 2011

  
• アンジオテンシンタイプ2受容体の発現と精製、および構造生物学のための構造認識抗体の作成(Expression and purification of Angiotensin receptor type 2 and production of structure recognition antibody for structural biology)

  浅田 秀基; 小林 貴美; 白石 充典; 島村 達郎; 植村 智子; 辻本 浩一; 飛田 直美; 中根 崇智; 岩成 宏子; 新井 修; 穐本 智; 中川 清; 浜窪 隆雄; 岩田 想; 小林 拓也

  日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, Dec. 2010

  
• アガリスク茸抽出液による免疫機能と抗腫瘍作用への影響(Effect of hot water extract from Agaricus blazei Murill on immue functions and anti-tumor activity)

  宮川 真由子; 廣野 由里子; 井上 慎一; 浅田 秀基; 松田 修; 竹内 亨; Pinkerton K.E.; 竹内 実

  日本癌学会総会記事, Sep. 2008

  
• 抗腫瘍免疫療法を目指したIL-12・ナノパーティクルの基礎検討

  清水健; 上田祐二; 岸田綱郎; 長谷川麗; 浅田秀基; 新屋政春; 秋吉一成; 松田修; 山岸久一

  Biotherapy (Tokyo), 2006

  
• 抗体を用いたプロスタグランジン受容体のX線結晶構造解析

  豊田洋輔; 森本和志; 寿野良二; 関口雄介; 山下恵太郎; 平田邦夫; 安田賢司; 安田賢司; 白石充典; 堀田韻虹; 浅田秀基; 中根崇智; 椎村祐樹; 中北智哉; 稲住知明; 告恭史郎; 梶原佑太; 梶原佑太; 清水朋子; 漆畑祐司; 吉田優; 栗原ともこ; 細谷孝充; 木下正弘; 杉本幸彦; 野村紀通; 村田武士; 高山喜好; 山本雅貴; 成宮周; 岩田想; 岩田想; 小林拓也

  GPCR研究会プログラム・抄録集, 2016

  
• Towards structure determination of the human prostanoid receptor bound to the antibody

  Y. Toyoda; K. Morimoto; R. Suno; Y. Sekiguchi; K. Yamashita; K. Hirata; S. Yasuda; H. Asada; T. Nakane; Y. Shiimura; T. Nakagita; T. Inazumi; K. Tsuge; Y. Kajiwara; T. Shimizu; Y. Urushibata; S. Yoshida; T. Kuribara; T. Hosoya; M. Kinoshita; Y. Sugimoto; N. Nomura; T. Murata; K. Takayama; M. Yamamoto; S. Narumiya; S. Iwata; T. Kobayashi

  GPCR workshop 2015, Dec. 2015

  
• プロスタノイド受容体の構造解析へのアプローチ

  豊田洋輔; 関口雄介; 浅田秀基; 寿野良二; 中根崇智; 椎村裕樹; 中北智哉; 中北智哉; 森本和志; 稲住知明; 告恭史郎; 杉本幸彦; 岩田想; 成宮周; 小林拓也

  GPCR研究会プログラム・抄録集, 2014

  
• Strategy for the structure determination of G protein-coupled receptor

  Takuya Kobayashi; Mitsunori Shiroishi; Hidetsugu Asada; Takami Yurugi; Tatsuro Shimamura; So Iwate

  JOURNAL OF PHARMACOLOGICAL SCIENCES, 2012

  
• Structure of the muscarinic acetylcholine receptor M2 subtype bound with an antagonist QNB (3-quinuclidinyl benzilate)

  Tatsuya Haga; Kazuko Haga; Andrew C. Kruse; Brian K. Kobilka; Hidetsugu Asada; Takuya Kobayashi

  JOURNAL OF PHARMACOLOGICAL SCIENCES, 2012

  
• Subcellular localization of EBNA1 and oriP that may support the high rate of exogenous gene transfer achieved using the EBV genetic elements

  Hidetsugu Asada; Shige H. Yoshimura; Tsunao Kishida; Jiro Imanishi; Osam Mazda

  2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, 2008, Peer-reviewed

  
• Cationic silica nanoparticles are efficiently transferred into mammalian cells

  Li Liu; Toshio Takenaka; Anatoly A. Zinchenko; Ning Chen; Shio Inagaki; Hidetsugu Asada; Tsunao Kishida; Osam Mazda; Shizuaki Murata; Kenichi Yoshikawa

  2007 INTERNATIONAL SYMPOSIUM ON MICRO-NANO MECHATRONICS AND HUMAN SCIENCE, VOLS 1 AND 2, 2007, Peer-reviewed

  
• Novel gene transduction procedures in vivo using the epstein-barr virus-based artificial chromosomes

  Osam Mazda; Tsunao Kishida; Hidetsugu Asada; Yasunori Iida; Sei Tsunoda; Yohei Oda; Shin-Ya Masaharu; Toshikazu Yoshikawa; Hiroaki Matsubara; Jiro Imanishi

  2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS, 2006, Peer-reviewed

  
• 喫煙と肺胞マクロファージの抗原提示機能

  竹内実; 浅田秀基; 長井苑子

  臨床免疫, 2005

  
• Intratumoral electrotransfer of interleukin-12 gene and bleomycin synergistically induced therapeutic immunity and suppressed subcutaneous and metastatic melanomas in mice

  T Kishida; H Asada; H Nakano; KS Yasutomi; M Shin-Ya; T Shinomiya; FD Cui; Y Hisa; J Imanishi; O Mazda

  MOLECULAR THERAPY, May 2004

  
• Dendritic cell (DC) vaccine loaded with apoptotic tumor fragments effectively suppress preestablished malignant melanoma in mice

  H Asada; T Kishida; H Hirai; J Imanishi; O Mazda

  MOLECULAR THERAPY, May 2004

  
• Intravascular insulin gene delivery as potential therapeutic intervention of the Diabetes Mellitus

  K Yasutomi; H Asada; T Kishida; FD Cui; T Takeuchi; J Imanishi; O Mazda

  MOLECULAR THERAPY, May 2003

  
• Anti-melanoma immune responses induced by autologous tumor cell vaccine engineered to express interleukin (IL)-12 and IL-18 by means of the EBV/Iipoplex

  H Asada; T Kishida; H Hirai; Y Iwakura; J Imanishi; O Mazda

  MOLECULAR THERAPY, May 2003

  
• Electroporation-mediated transfer of the short interfering RNA induced sequence specific genetic silencing in the murine skeletal muscle

  T Kishida; H Asada; J Imanishi; O Mazda

  MOLECULAR THERAPY, May 2003

  
• The Epstein-Barr virus (EBV)/lipoplex-mediated transfection of interleukin (IL)-12 and IL-18 genes augments T helper 1 (Th1) immune responses and therapeutic anti-melanoma effects of autologous tumor vaccine

  Asada H; Kishida T; Satoh E; Hirai H; Imanishi J; Mazda O

  5th Annual Meeting of the American Society of Gene Therapy, May 2002

  

Books and Other Publications

• Muscarinic Receptor: From Structure to Animal Models

  寿野良二; 浅田秀基; 小林拓也, Joint work, Towards the Crystal Structure Determination of Muscarinic Acetylcholine Receptors

  Humana Press, Aug. 2016, Not refereed

  
• 膜タンパク質構造研究

  浅田秀基; 小林拓也, Joint work, 昆虫細胞による生産

  株式会社化学同人, Oct. 2013, Not refereed

  
• 別冊・医学のあゆみ 免疫疾患 state of arts Ver.2

  浅田秀基; 岸田綱郎; 今西二郎; 松田修, Joint work, サイトカイン遺伝子による免疫遺伝子治療

  医歯薬出版株式会社, Mar. 2002, Not refereed

  

Industrial Property Rights

• 特開2019-203019, 特願2019-151308, 抗BRIL抗体及び該抗体を用いたBRIL融合タンパク質の安定化方法

  宮城 光; 鈴木 道彦; 齋藤 純一; 浅田 秀基; 岩田 想

  
• 特許第6577691号, 特願2019-515561, 抗BRIL抗体及び該抗体を用いたBRIL融合タンパク質の安定化方法

  宮城 光; 鈴木 道彦; 齋藤 純一; 浅田 秀基; 岩田 想

  
• WO2019-013308, JP2018026408, 抗BRIL抗体及び該抗体を用いたBRIL融合タンパク質の安定化方法

  宮城 光; 鈴木 道彦; 齋藤 純一; 浅田 秀基; 岩田 想

  
• 特開2005-295809, 特願2004-112163, cDNA発現ミュージアム

  松田 修; 岸田 綱郎; 浅田 秀基; 今西 二郎

  
• 特開2004-059574, 特願2003-124732, インターロイキン21を用いた免疫治療用医薬組成物

  岸田 綱郎; 浅田 秀基; 今西 二郎; 松田 修

  

External funds: Kakenhi

• Activation mechanism of G protein-coupled receptors by X-ray free electron lasers

  Grant-in-Aid for Scientific Research (B)

  Basic Section 43020:Structural biochemistry-related

  Tohoku University;Institute of Physical and Chemical Research

  Eriko Nango

  From 01 Apr. 2018, To 31 Mar. 2021, Project Closed

  X線結晶構造解析;Gタンパク質共役型受容体;X線自由電子レーザー;シリアルフェムト秒結晶構造解析;ロドプシン;Ｘ線自由電子レーザー

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18H02394/
• Crystallization of Angiotensin receptor type 2 with its specific antibody

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Hidetsugu Asada

  From 01 Apr. 2013, To 31 Mar. 2016, Project Closed

  Gタンパク質共役受容体;X線結晶構造解析;膜蛋白質;アンジオテンシン;X線結晶構造;構造認識抗体;アンジオテンシン受容体;構造解析;GPCR;膜タンパク質

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25293068/
• Structure determination of histamine H1 receptor

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Tatsuro SHIMAMURA

  From 01 Apr. 2012, To 31 Mar. 2015, Project Closed

  アレルギー;花粉症;GPCR;結晶構造解析;ヒスタミン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24370044/
• Challenges for the structure determination of GPCR active conformation

  Grant-in-Aid for Challenging Exploratory Research

  Kyoto University

  Tatsuro SHIMAMURA

  From 01 Apr. 2011, To 31 Mar. 2013, Project Closed

  X線結晶解析;受容体;GPCR;膜タンパク質;構造解析;G蛋白質共役型受容体;X線結晶構造解析

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23657074/
• Crystallization of GPCR and its specific antibody

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Hidetsugu ASADA

  From 01 Apr. 2010, To 31 Mar. 2013, Project Closed

  構造生物;GPCR;抗体;結晶化;X線結晶構造解析;共結晶;構造解析

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590270/
• RNA interference-based therapeutic molecular targeting strategy using nano drug delivery systems to eradicate malignant melanoma

  Grant-in-Aid for Scientific Research (C)

  Kyoto Prefectural University of Medicine

  Kishimoto SABURO

  From 01 Apr. 2007, To 31 Mar. 2009, Project Closed

  悪性黒色腫;分子標的治療;RNAインタフェレンス;サイトカイン;免疫療法;RNAi;DDS;癌;免疫学

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19591323/
• グレリン受容体のシグナル伝達選択機構の構造基盤

  Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields

  Kyoto University

  浅田 秀基

  From 07 Oct. 2022, To 31 Mar. 2026, Granted

  Gタンパク質共役受容体;グレリン受容体;バイスシグナリング;クライオ電子顕微鏡

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KK0099/
• デザイナーリガンドで制御可能な受容体の創成および生成的デザイン

  Grant-in-Aid for Transformative Research Areas (A)

  Transformative Research Areas, Section (II)

  Nagoya University

  清中 茂樹

  From 01 Apr. 2024, To 31 Mar. 2029, Granted

  受容体;Gタンパク質共役型受容体;X線結晶構造解析;人工受容体

  https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-24H02265/
• 細胞膜受容体の動的構造解析と活性化機構の解明

  Grant-in-Aid for Scientific Research (A)

  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields

  Tohoku University

  南後 恵理子

  From 01 Apr. 2024, To 31 Mar. 2027, Granted

  X線自由電子レーザー;時分割結晶構造解析;動的構造

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24H00547/