Basic Information

Academic Degree

• 修士（工学）(大阪大学)
• 博士（工学）(大阪大学)

Research History

• From Apr. 2013, To Present

  RIKEN, 光量子工学研究領域, 研究員
• From Apr. 2012, To Mar. 2013

  RIKEN, 基幹研究所, 基幹研究所研究員
• From Apr. 2011, To Mar. 2012

  RIKEN, 緑川レーザー物理工学研究室, 協力研究員
• From Apr. 2008, To Mar. 2011

  RIKEN, 緑川レーザー物理工学研究室, 基礎科学特別研究員
• From Apr. 2007, To Mar. 2008

  RIKEN, 緑川レーザー物理工学研究室, 協力研究員

ID,URL

• J-Global ID

  201401069200604405

• External link

  http://kaken.nii.ac.jp/d/r/10462657.ja.html

researchmap URL

• https://researchmap.jp/kisobe

Research

Research Topics, Overview of the research

• Research Topics

  Development of novel imaging and optical manipulation techniques by spatio-temporal control of light

• Overview of the research

  Two-photon fluorescence microscopy is a powerful tool for investigating biological phenomena in biological tissues. However, imaging characteristics beyond those of commercially available two-photon fluorescence microscopes are required. In this laboratory, we develop novel imaging and optical manipulation techniques for investigating biological phenomena.

Research Interests

• 非線形光学顕微鏡
• 深部イメージング
• 超解像イメージング
• 非線形光学分光
• 変調

Research Areas

• Manufacturing technology (mechanical, electrical/electronic, chemical engineering), Measurement engineering
• Life sciences, Biomedical engineering
• Nanotechnology/Materials, Optical engineering and photonics

Papers

• Posteromedial cortical networks encode visuomotor prediction errors.

  Ryosuke F. Takeuchi; Akinori Y. Sato; Kei N. Ito; Hiroshi Yokoyama; Reiji Miyata; Rumina Ueda; Konosuke Kitajima; Riki Kamaguchi; Toshiaki Suzuki; Keisuke Isobe; Naoki Honda; Fumitaka Osakada

  16 Aug. 2022

  
• Multifocal-plane multiphoton fluorescence microscopy and multiphoton pattered illumination

  Keisuke Isobe

  Photonics Review, 22 May 2024, Lead author, Last author, Corresponding author

  
• Deep imaging techniques by spatio-temporal control of excitation pulses

  Keisuke Isobe; Kana Namiki; Takayuki Michikawa; Atsushi Miyawaki; Fumitaka Osakada; Katsumi Midorikaw

  Proceedings of SPIE - The International Society for Optical Engineering, 2019

  
• Simultaneous two-photon absorption and stimulated Raman scattering imaging by spatial overlap modulation microscopy

  K. Isobe; H. Kawano; A. Suda; A. Kumagai; A. Miyawaki; K. Midorikawa

  Optics InfoBase Conference Papers, 2013

  
• 多焦点面を同時観察するための多光子蛍光顕微鏡と多光子パターン照明

  磯部圭佑

  フォトニクスニュース, 10 Mar. 2023

  
• Fast z-focus controlling and multiplexing strategies for multiplane two-photon imaging of neural dynamics.

  Kei N Ito; Keisuke Isobe; Fumitaka Osakada

  Neuroscience research, 31 Mar. 2022

  
• Spatial Overlap Modulation Nonlinear Optical Microscopy for Background-Free Deep Imaging

  Isobe Keisuke; Midorikawa Katsumi

  Nippon Laser Igakkaishi, 2015

  
• Nonlinear Optical Microscopy Employing Ultra-Broadband Femtosecond Laser Pulses

  KANNARI Fumihiko; ISOBE Keisuke; SUDA Akira; HASHIMOTO Hiroshi; KAWANO Hiroyuki; MIZUNO Hideaki; MIYAWAKI Atsushi; MIDORIKAWA Katsumi

  rle, 15 Dec. 2011

  
• Control of Nano-Scale Plasmon in Time and Space using Pulse Shaped Femtosecond Laser

  T. Harada; K. Matsuishi; N. Sugiura; Y. Oishi; K. Isobe; A. Suda; H. Mizuno; A. Miyawaki; K. Midorikawa; F. Kannari

  2010 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO) AND QUANTUM ELECTRONICS AND LASER SCIENCE CONFERENCE (QELS), 2010, Peer-reviewed

  
• Bioimaging by fourier-transform coherent anti-stokes Raman scattering microscopy

  K. Isobe; A. Suda; H. Hashimoto; F. Kannari; H. Kawano; H. Mizuno; A. Miyawaki; K. Midorikawa

  CLEO/Europe - EQEC 2009 - European Conference on Lasers and Electro-Optics and the European Quantum Electronics Conference, 2009, Peer-reviewed

  
• Adaptive optics with spatio-temporal lock-in detection for temporal focusing microscopy

  Tomohiro Ishikawa; Keisuke Isobe; Kenta Inazawa; Kana Namiki; Atsushi Miyawaki; Fumihiko Kannari; Katsumi Midorikawa

  Optics Express, 30 Aug. 2021, Peer-reviewed, Corresponding author

  
• Enhancement of optical sectioning capability of temporal focusing microscopy by using time-multiplexed multi-line focusing

  Kenta Inazawa; Keisuke Isobe; Tomohiro Ishikawa; Kana Namiki; Atsushi Miyawaki; Fumihiko Kannari; Katsumi Midorikawa

  Applied Physics Express, 01 Aug. 2021, Peer-reviewed, Corresponding author

  
• Temporally multiplexed dual-plane imaging of neural activity with four-dimensional precision.

  Masanari Onda; Ryosuke F Takeuchi; Keisuke Isobe; Toshiaki Suzuki; Yuji Masaki; Nao Morimoto; Fumitaka Osakada

  Neuroscience research, 16 Feb. 2021, Peer-reviewed

  
• Resistance to optical distortions in three-dimensional interferometric temporal focusing microscopy

  Qiyuan Song; Keisuke Isobe; Katsumi Midorikawa; Fumihiko Kannari

  OPTICS COMMUNICATIONS, Jan. 2019, Peer-reviewed

  
• Interferometric temporal focusing microscopy using three-photon excitation fluorescence.

  Toda K; Isobe K; Namiki K; Kawano H; Miyawaki A; Midorikawa K

  Biomedical optics express, Apr. 2018, Peer-reviewed, Corresponding author

  
• Temporal focusing microscopy using three-photon excitation fluorescence with a 92-fs Yb-fiber chirped pulse amplifier

  Keisuke Toda; Keisuke Isobe; Kana Namiki; Hiroyuki Kawano; Atsushi Miyawaki; Katsumi Midorikawa

  BIOMEDICAL OPTICS EXPRESS, Jun. 2017, Peer-reviewed, Corresponding author

  
• Temporal focusing microscopy combined with three-dimensional structured illumination

  Keisuke Isobe; Keisuke Toda; Qiyuan Song; Fumihiko Kannari; Hiroyuki Kawano; Atsushi Miyawaki; Katsumi Midorikawa

  JAPANESE JOURNAL OF APPLIED PHYSICS, May 2017, Peer-reviewed

  
• Multiphoton 3D structured illumination microscopy for enhanced axial resolution in deep imaging

  Qiyuan Song; Keisuke Isobe; Fumihiko Kannari; Hiroyuki Kawano; Akiko Kumagai; Atsushi Miyawaki; Katsumi Midorikawa

  2015 11th Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2015, 07 Jan. 2016, Peer-reviewed

  
• Two-dimensional spatiotemporal focusing of femtosecond pulses and its applications in microscopy

  Qiyuan Song; Aoi Nakamura; Kenichi Hirosawa; Keisuke Isobe; Katsumi Midorikawa; Fumihiko Kannari

  REVIEW OF SCIENTIFIC INSTRUMENTS, Aug. 2015, Peer-reviewed

  
• 2D simultaneous spatial and temporal focusing multiphoton microscopy for fast volume imaging with improved sectioning ability

  Qiyuan Song; Keisuke Isobe; Kenichi Hirosawa; Katsumi Midorikawa; Fumihiko Kannari

  Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2015, Peer-reviewed

  
• 2D simultaneous spatial and temporal focusing multiphoton microscopy for fast volume imaging with improved sectioning ability

  Qiyuan Song; Keisuke Isobe; Kenichi Hirosawa; Katsumi Midorikawa; Fumihiko Kannari

  MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XV, 2015, Peer-reviewed

  
• Temporal focusing microscopy with structured illumination for super-resolution deep imaging

  Keisuke Isobe; Kyohei Mochizuki; Qiyuan Song; Akira Suda; Fumihiko Kannari; Hiroyuki Kawano; Akiko Kumagai; Atsushi Miyawaki; Katsumi Midorikawa

  2014 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO), 2014, Peer-reviewed

  
• Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination

  Keisuke Isobe; Takanori Takeda; Kyohei Mochizuki; Qiyuan Song; Akira Suda; Fumihiko Kannari; Hiroyuki Kawano; Akiko Kumagai; Atsushi Miyawaki; Katsumi Midorikawa

  BIOMEDICAL OPTICS EXPRESS, Nov. 2013, Peer-reviewed, Lead author, Corresponding author

  
• Implementation of spatial overlap modulation nonlinear optical microscopy using an electro-optic deflector

  Keisuke Isobe; Hiroyuki Kawano; Akiko Kumagai; Atsushi Miyawaki; Katsumi Midorikawa

  BIOMEDICAL OPTICS EXPRESS, Oct. 2013, Peer-reviewed, Lead author, Corresponding author

  
• Simultaneous imaging of two-photon absorption and stimulated raman scattering by spatial overlap modulation nonlinear optical microscopy

  Keisuke Isobe; Hiroyuki Kawano; Akira Suda; Atsushi Miyawaki; Atsushi Miyawaki; Katsumi Midorikawa

  Biomedical Optics Express, 01 Sep. 2013, Peer-reviewed, Lead author, Corresponding author

  
• Background-free deep imaging by spatial overlap modulation nonlinear optical microscopy

  Keisuke Isobe; Hiroyuki Kawano; Takanori Takeda; Akira Suda; Akiko Kumagai; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

  BIOMEDICAL OPTICS EXPRESS, Jul. 2012, Peer-reviewed, Lead author, Corresponding author

  
• Spatial overlap modulation nonlinear optical microscopy

  Keisuke Isobe; Hiroyuki Kawano; Takanori Takeda; Akira Suda; Akiko Kumagai; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

  2012 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO), 2012, Peer-reviewed

  
• Temporal control of local plasmon distribution on Au nanocrosses by ultra-broadband femtosecond laser pulses and its application for selective two-photon excitation of multiple fluorophores

  Takuya Harada; Keiichiro Matsuishi; Yu Oishi; Keisuke Isobe; Akira Suda; Hiroyuki Kawan; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa; Fumihiko Kannari

  OPTICS EXPRESS, Jul. 2011, Peer-reviewed

  
• High-resolution fluorescence microscopy based on a cyclic sequential multiphoton process

  Keisuke Isobe; Akira Suda; Hiroshi Hashimoto; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

  Biomedical Optics Express, 01 Oct. 2010, Peer-reviewed, Lead author, Corresponding author

  
• Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy

  Keisuke Isobe; Hiroshi Hashimoto; Akira Suda; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

  Biomedical Optics Express, 01 Sep. 2010, Peer-reviewed, Lead author, Corresponding author

  
• Nonlinear Optical Microscopy and Spectroscopy Employing Octave Spanning Pulses

  Keisuke Isobe; Akira Suda; Masahiro Tanaka; Hiroshi Hashimoto; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

  IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, Jul. 2010, Peer-reviewed, Invited, Lead author, Corresponding author

  
• Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy.

  Hashimoto H; Isobe K; Suda A; Kannari F; Kawano H; Mizuno H; Miyawaki A; Midorikawa K

  Applied optics, Jun. 2010, Peer-reviewed

  
• High-resolution fluorescence microscopy employing a cyclic sequential multiphoton excitation

  Keisuke Isobe; Akira Suda; Hiroshi Hashimoto; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

  2010 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO) AND QUANTUM ELECTRONICS AND LASER SCIENCE CONFERENCE (QELS), 2010, Peer-reviewed

  
• Multifarious control of two-photon excitation of multiple fluorophores achieved by phase modulation of ultra-broadband laser pulses

  Keisuke Isobe; Akira Suda; Masahiro Tanaka; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

  OPTICS EXPRESS, Aug. 2009, Peer-reviewed, Lead author, Corresponding author

  
• Single-pulse coherent anti-Stokes Raman scattering microscopy employing an octave spanning pulse

  Keisuke Isobe; Akira Suda; Masahiro Tanaka; Hiroshi Hashimoto; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

  OPTICS EXPRESS, Jul. 2009, Peer-reviewed, Lead author, Corresponding author

  
• Measurement of two-photon excitation spectrum of various fluorophores with Fourier transform nonlinear spectroscopy

  Hiroshi Hashimoto; Keisuke Isobe; Akira Suda; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

  2009 CONFERENCE ON LASERS AND ELECTRO-OPTICS AND QUANTUM ELECTRONICS AND LASER SCIENCE CONFERENCE (CLEO/QELS 2009), VOLS 1-5, 2009, Peer-reviewed

  
• Selective excitation in nonlinear microscopy by using an ultra-broadband pulse

  Keisuke Isobe; Akira Suda; Masahiro Tanaka; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

  ULTRAFAST PHENOMENA XVI, 2009, Peer-reviewed

  
• Multifarious Two-Photon Fluorescence Microscopy Employing Ultrabroadband Femtosecond Laser Pulses

  Keisuke Isobe; Akira Suda; Hiroshi Hashimoto; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

  FRONTIERS IN PHYSICS-BOOK, 2009, Peer-reviewed

  
• Multifarious control of two-photon fluorescence intensities in multi-labeled cell using a single ultrabroadband light source

  Keisuke Isobe; Akira Suda; Hiroshi Hashimoto; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

  2009 CONFERENCE ON LASERS AND ELECTRO-OPTICS AND QUANTUM ELECTRONICS AND LASER SCIENCE CONFERENCE (CLEO/QELS 2009), VOLS 1-5, 2009, Peer-reviewed

  
• Fourier-transform spectroscopy combined with a 5-fs broadband pulse for multispectral nonlinear microscopy

  Keisuke Isobe; Akira Suda; Masahiro Tanaka; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

  PHYSICAL REVIEW A, Jun. 2008, Peer-reviewed, Lead author, Corresponding author

  
• Three-dimensional profiling of refractive index distribution inside transparent materials by use of nonresonant four-wave mixing microscopy

  Keisuke Isobe; Takehito Kawasumi; Takayuki Tamaki; Shogo Kataoka; Yasuyuki Ozeki; Kazuyoshi Itoh

  APPLIED PHYSICS EXPRESS, Feb. 2008, Peer-reviewed, Lead author

  
• Selective excitation of vibrational mode and Fourier-transform four-wave-mixing spectroscopy using an ultrabroadband laser

  Keisuke Isobe; Akira Suda; Masahiro Tanaka; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

  2008 CONFERENCE ON LASERS AND ELECTRO-OPTICS & QUANTUM ELECTRONICS AND LASER SCIENCE CONFERENCE, VOLS 1-9, 2008, Peer-reviewed

  
• Coherent control of two-photon excited fluorescence with ultrabroadband optical pulses

  Masahiro Tanaka; Keisuke Isobe; Akira Suda; Fumihiko Kannari; Hiroyuki Kawan; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

  2008 CONFERENCE ON LASERS AND ELECTRO-OPTICS & QUANTUM ELECTRONICS AND LASER SCIENCE CONFERENCE, VOLS 1-9, 2008, Peer-reviewed

  
• Histone H2A mobility is regulated by its tails and acetylation of core histone tails

  Tsunehito Higashi; Sachihiro Matsunaga; Keisuke Isobe; Akihiro Morimoto; Tomoko Shimada; Shogo Kataoka; Wataru Watanabe; Susumu Uchiyama; Kazuyoshi Itoh; Kiichi Fukui

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Jun. 2007, Peer-reviewed

  
• Single-organelle tracking by two-photon conversion

  Wataru Watanabe; Tomoko Shimada; Sachihiro Matsunaga; Daisuke Kurihara; Kiichi Fukui; Shin-ichi Arimura; Nobuhiro Tsutsumi; Keisuke Isobe; Kazuyoshi Itoh

  OPTICS EXPRESS, Mar. 2007, Peer-reviewed

  
• Estimation of refractive index distribution inside transparent materials by use of four-wave mixing process

  Takehito Kawasumi; Keisuke Isobe; Takayuki Tamaki; Shogo Kataoka; Yasuyuki Ozeki; Kazuyoshi Itoh

  2007 CONFERENCE ON LASERS & ELECTRO-OPTICS/QUANTUM ELECTRONICS AND LASER SCIENCE CONFERENCE (CLEO/QELS 2007), VOLS 1-5, 2007, Peer-reviewed

  
• Highly sensitive spectral interferometric four-wave mixing microscopy near the shot noise limit and its combination with two-photon excited fluorescence microscopy

  Keisuke Isobe; Yasuyuki Ozeki; Takehito Kawasumi; Shogo Kataoka; Shin'ichiro Kajiyama; Kiichi Fukui; Kazuyoshi Itoh

  OPTICS EXPRESS, Nov. 2006, Peer-reviewed

  
• Coherence-based 3-D and spectral imaging and laser-scanning microscopy

  K Itoh; W Watanabe; H Arimoto; K Isobe

  PROCEEDINGS OF THE IEEE, Mar. 2006, Peer-reviewed, Invited

  
• Spectral interference technique in stimulated parametric emission microscopy

  Kazuyoshi Itoh; Keisuke Isobe

  OPTICAL INFORMATION SYSTEMS IV, 2006, Peer-reviewed

  
• Stimulated parametric emission microscope systems

  Kazuyoshi Itoh; Keisuke Isobe

  INFORMATION OPTICS, 2006, Peer-reviewed

  
• Stimulated parametric emission microscopy

  K Isobe; S Kataoka; R Murase; W Watanabe; T Higashi; S Kawakami; S Matsunaga; K Fukui; K Itoh

  OPTICS EXPRESS, Jan. 2006, Peer-reviewed

  
• Intracellular disruption of mitochondria in a living HeLa cell with a 76-MHz femtosecond laser oscillator

  T Shimada; W Watanabe; S Matsunaga; T Higashi; H Ishii; K Fukui; K Isobe; K Itoh

  OPTICS EXPRESS, Nov. 2005, Peer-reviewed

  
• Dynamic analysis of EGFP-histone h1 by two-photon FRAP

  Tsunehito Higashi; Keisuke Isobe; Wataru Watanabe; Susumu Uchiyama; Sachihir Matsunaga; Kazuyoshi Itoh; Kiichi Fukui

  CELL STRUCTURE AND FUNCTION, Jun. 2005, Peer-reviewed

  
• Stimulated parametric fluorescence microspectroscopy

  K. Isobe; R. Murase; S. Kataoka; W. Watanabe; S. Kawakami; S. Matsunaga; T. Higashi; K. Fukui; K. Itoh

  Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS, 2005, Peer-reviewed

  
• Stimulated parametric fluorescence microspectroscopy

  K Isobe; R Murase; S Kataoka; W Watanabe; S Kawakami; S Matsunaga; T Higashi; K Fukui; K Itoh

  2005 IEEE LEOS Annual Meeting Conference Proceedings (LEOS), 2005, Peer-reviewed

  
• Multi-spectral two-photon excited fluorescence microscopy using supercontinuum light source

  K Isobe; W Watanabe; S Matsunaga; T Higashi; K Fukui; K Itoh

  JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS & EXPRESS LETTERS, 2005, Peer-reviewed

  
• Femtosecond laser disruption of subcellular organelles in a living cell

  W Watanabe; N Arakawa; S Matsunaga; T Higashi; K Fukui; K Isobe; K Itoh

  OPTICS EXPRESS, Sep. 2004, Peer-reviewed

  
• Fringe- and speckle-free holographic patterned illumination using time-multiplexed temporal focusing

  Tomohiro Ishikawa; Keisuke Isobe; Kenta Inazawa; Takayuki Michikawa; Kana Namiki; Atsushi Miyawaki; Fumihiko Kannari; Katsumi Midorikawa

  Applied Physics Express, 28 Mar. 2022, Peer-reviewed, Corresponding author

  

Misc.

• 四光波混合過程を利用する透明材料内部の三次元屈折率分布推定

  川澄健人; 磯部圭佑; 玉木隆幸; 片岡省吾; 小関泰之; 伊東一良

  日本分光学会講演要旨集, 2006

  
• Super-Resolution Deep Imaging by Spatio-Temporal Control of Excitation Pulses

  磯部 圭佑; 戸田 圭亮; 緑川 克美

  レーザー研究 = The review of laser engineering : レーザー学会誌, Oct. 2016

  
• Nobel nonlinear optical microscopy for super-resolution deep imaging

  ISOBE K.; KAWANO H.; TAKEDA T.; SUDA A.; KUMAGAI A.; MIYAWAKI A.; MIDORIKAWA K.

  レーザー学会研究会報告 = Reports on the Topical meeting of the Laser Society of Japan, 19 Dec. 2011

  
• Measurement of two-photon excitation spectra of fluorescent proteins with Fourier-transform nonlinear spectroscopy

  SUDA A.; ISOBE K.; HASHIMOTO H.; KANNARI F.; KAWANO H.; MIZUNO H.; MIYAWAKI A.; MIDORIKAWA K.

  レーザー学会研究会報告 = Reports on the Topical meeting of the Laser Society of Japan, 13 Sep. 2010

  
• Nonlinear optical microscopy employing spectral phase modulation

  ISOBE Keisuke; SUDA Akira; KAWANO Hiroyuki; MIZUNO Hideaki; MIYAWAKI Atsushi; HASHIMOTO Hiroshi; KANNARI Fumihiko; MIDORIKAWA Katsumi

  電気学会研究会資料. OQD, 光・量子デバイス研究会, 13 Feb. 2009

  
• 1H16-4 Subcellular dynamics analysis of proteins by two-photon FRAP

  HIGASHI Tsunehito; ISOBE Keisuke; WATANABE Wataru; MATSUNAGA Sachhiro; ITOH Kazuyoshi; FUKUI Kiichi

  日本生物工学会大会講演要旨集, 2005

  
• 3S2-AM2 Visualiztion of bio-molecules in living cells by laser 3D microscopy

  FUKUI Kiichi; MATSUNAGA Sachihiro; HIGASHI Tsunehito; ISOBE Keisuke; WATANABE Wataru; ITOH Kazuyoshi

  日本生物工学会大会講演要旨集, 2004

  
• Analyses of fluorescence proteins by multiple-wavelength excitation fluorescence microscopy using supercontinuum

  Kiichi Fukui; Sachihiro Mastunaga; Keisuke Isobe; Tsunehito Higashi; Wataru Watanabe; Kazuyoshi Itoh

  CELL STRUCTURE AND FUNCTION, May 2004

  
• Deep imaging by spatial overlap modulation nonlinear optical microscopy

  磯部 圭佑; 緑川 克美

  Optronics : 光技術コーディネートジャーナル, Aug. 2016

  
• 非線形光学イメージング技術の発展

  磯部 圭佑

  分光研究 = Journal of the spectroscopical research of Japan, 15 Oct. 2013

  

Presentations

• 脳機能解明のためのイメージング技術と光操作技術

  磯部 圭佑; 稲澤 健太; 道川 貴章; 宮脇 敦史; 緑川 克美

  第12回RAPシンポジウム, 11 Dec. 2024

  
• 光刺激中の多光子イメージング技術

  磯部 圭佑; 稲澤 健太; 道川 貴章; 宮脇 敦史; 緑川 克美

  第85回応用物理学会秋季学術講演会, 19 Sep. 2024

  
• Dorsal cortical pathway for visuomotor predictive coding in the brain

  Ryosuke F. Takeuchi; Akinori Y. Sato; Kei N. Ito; Hiroshi Yokoyama; Reiji Miyata; Rumina Ueda; Konosuke Kitajima; Riki Kamaguchi; Masahiro Yamaguchi; Toshiaki Suzuki; Keisuke Isobe; Naoki Honda; Fumitaka Osakada

  NEURO2024, 27 Jul. 2024

  
• 脳機能の可視化と操作に向けたフェムト秒レーザー技術の開発

  磯部 圭佑

  理研 RAP ・弘前大理工合同シンポジウム, 15 Apr. 2024

  
• Simultaneous multi-plane two-photon imaging and 4D optical manipulation

  Keisuke Isobe

  Laser Display and Lighting Conference 2024 (LDC2024), 26 Apr. 2024, Invited

  
• Scanless three-dimensional photostimulation with single cell resolution using time-multiplexed multi-line temporal focusing

  Kenta Inazawa; Keisuke Isobe; Takayuki Michikawa; Kana Namiki; Atsushi Miyawaki; Katsumi Midorikawa

  The 10th Biomedical Imaging and Sensing Conference BISC 2024, 25 Apr. 2024

  
• 単一細胞分解能を有する非走査３次元多光子光刺激

  稲澤 健太; 磯部 圭佑; 道川 貴章; 並木 香奈; 宮脇 敦史; 緑川 克美

  第71回応用物理学会春季講演会, 23 Mar. 2024

  
• 多細胞のための時間多重化マルチライン時空間集光多光子光刺激

  稲澤 健太; 磯部 圭佑; 道川 貴章; 並木 香奈; 宮脇 敦史; 緑川 克美

  レーザー学会学術講演会第44回年次大会, 19 Jan. 2024

  
• 多焦点面同時多光子蛍光イメージングのためのフェムト秒レーザー開発

  磯部 圭佑; 伊藤 慶; 佐藤 彰典; 竹内 遼介; 小坂田 文隆; 緑川 克美

  レーザー学会学術講演会第44回年次大会, 17 Jan. 2024

  
• 大規模カルシウムイメージングによる脳活動リズムと身体運動リズムの解析

  道川 貴章; 磯部 圭佑

  第10回日本BMI研究会, 11 Nov. 2023

  
• Scanless two-photon patterned illumination with high axial resolution

  Kenta Inazawa; Keisuke Isobe; Takayuki Michikawa; Kana Namiki; Atsushi Miyawaki; Katsumi Midorikawa

  The 12th Asia-Pacific Laser Symposium, 05 Sep. 2023

  
• Large-scale simultaneous measurements of mouse cerebral and cerebellar activity during voluntary movements

  T. Michikawa; K. Isobe; A. Miyawaki; K. Midorikawa

  理研シンポジウム：第10回「光量子工学研究」, 20 Dec. 2022

  
• 随意運動中のマウス大脳および小脳活動のカルシウムイメージングによる大規模同時計測

  道川 貴章; 磯部 圭佑; 宮脇 敦史; 緑川 克美

  第 9 回日本 BMI 研究会, 22 Oct. 2022

  
• 時間多重化時空間集光を用いた３次元多光子パターン照明技術

  磯部 圭佑; 道川 貴章; 並木 香奈; 宮脇 敦史; 緑川 克美

  第83回応用物理学会秋季講演会, 21 Sep. 2022

  
• Adaptive optics for temporal focusing and two-photon patterned illumination

  K. Isobe

  International;Symposium on Comprehensive understanding of scattering;fluctuated fields;science of clairvoyance, 22 Apr. 2022, Invited

  
• Fringe- and speckle-free holographic patterned illumination using multifocal time-multiplexed temporal focusing pulse

  K. Isobe; T. Ishikawa; K. Inazawa; T. Michikawa; F. Kannari; K. Midorikawa

  The 11th Advanced Lasers and Photon Sources, 18 Apr. 2022

  
• 超短光パルスの時空間制御による多光子イメージングと光操作

  磯部圭佑; 緑川 克美

  レーザー学会学術講演会第42回年次大会, 14 Jan. 2022

  
• 大規模カルシウムイメージングによるマウス大脳および小脳における感覚情報表現の解析

  道川 貴章; 磯部 圭佑; 宮脇 敦史; 緑川 克美

  第 8 回日本 BMI 研究会, 06 Nov. 2021

  
• 多平面同時多光子イメージングと多光子パターン照明

  磯部圭佑

  レーザー顕微鏡研究会, 05 Nov. 2021, Invited

  
• Large-Scale Optical Imaging of Neural Activity in the Mouse Cerebrum and Cerebellum

  T. Michikawa; K. Isobe; A. Miyawaki; K. Midorikawa

  EMBO | EMBL Symposium, Seeing is Believing: Imaging the Molecular Processes of Life, 06 Oct. 2021

  
• Background suppression of structured illumination microscopy by using time-multiplexed multi-line temporal-focusing

  K. Isobe; K. Inazawa; T. Ishikawa; K. Namiki; A. Miyawaki; F. Kannari; K. Midorikawa

  EMBO | EMBL Symposium, Seeing is Believing: Imaging the Molecular Processes of Life, 05 Oct. 2021

  
• 多焦点面同時多光子イメージング技術の開発

  磯部 圭佑; 恩田 将成; 竹内 遼介; 伊藤 慶; 佐藤 彰典; 道川 貴章; 宮脇 敦史; 小坂田 文隆; 緑川 克美

  第82回応用物理学会秋季講演会, 21 Sep. 2021

  
• 超短光パルスの時空間分布制御による多光子イメージングと光操作技術

  磯部圭佑

  強光子場科学研究懇談会, 09 Jul. 2021, Invited

  
• Enhancement of axial resolution of temporal focusing microscopy by using programmable time-multiplexed multi-line focusing

  K. Isobe; K. Inazawa; T. Ishikawa; F. Kannari; K. Namiki; A. Miyawaki; K. Midorikawa

  The 10th Advanced Lasers and Photon Sources, 21 Apr. 2021

  
• Adaptive optics of wide-field temporal focusing microscopy with structured illumination microscopy

  T. Ishikawa; K. Isobe; K. Inazawa; F. Kannari; K. Midorikawa

  The 10th Advanced Lasers and Photon Sources, 21 Apr. 2021

  
• Transient dynamics of an ANDi mode-locked Yb-fiber laser oscillator monitored by time-stretch dispersive Fourier transform

  T. Ishikawa; K. Isobe; M. Yoshizawa; F. Kannari; K. Midorikawa

  The 10th Advanced Lasers and Photon Sources, 19 Apr. 2021

  

Industrial Property Rights

• 特許第5137026号, 特開2010-014677, 特願2008-177267, 2光子励起蛍光観察方法及び装置

  磯部 圭佑; 須田 亮; 緑川 克美; 神成 文彦; 宮脇 敦史

  
• WO2012-127907, JP2012052377, 非線形光学顕微鏡および非線形光学顕微鏡法

  磯部 圭佑; 緑川 克美

  
• 特開2011-145487, 特願2010-006090, 多光子励起顕微鏡

  磯部 圭佑; 緑川 克美; 須田 亮; 神成 文彦; 宮脇 敦史

  
• 特開2009-150649, 特願2005-095096, 空間情報検出装置

  伊東 一良; 磯部 圭佑; 福井 希一; 渡辺 歴; 片岡 省吾

  
• 特開2008-116409, 特願2006-302038, 屈折率測定方法および屈折率測定装置、光学部材の製造方法

  伊東 一良; 小関 泰之; 磯部 圭佑; 玉木 隆幸; 片岡 省吾; 川澄 健人

  
• 特許第3885574号, 特開2003-172689, 特願2001-372569, 高調波干渉式分析方法及び装置

  伊東 一良; 福井 希一; 渡邉 歴; 磯部 圭佑; 大久保 邦彦

  
• WO2006-104237, JP2006307004, 空間情報検出装置

  伊東 一良; 磯部 圭佑; 福井 希一; 渡邉 歴; 片岡 省吾

  
• 特開2006-010523, 特願2004-188504, 分子測定装置

  大久保 邦彦; 伊東 一良; 福井 希一; 磯部 圭佑; 隅田 剣生

  
• 特開2005-172776, 特願2003-436342, 複数の波長依存性物質を含む試料の分析方法及び分析装置

  伊東 一良; 福井 希一; 渡邉 歴; 磯部 圭佑

  

Awards

• 02 Sep. 2008

  （社）応用物理学会, 応用物理学会講演奨励賞
• （独）理化学研究所, POSTER AWARD ENGINEERING PRIZE at 2010 special postdoctoral researcher (SPDR) and foreign postdoctoral researcher (FPR) presentation of research results
• 11 Mar. 2011

  （独）理化学研究所, 研究奨励賞
• 07 Jan. 2011

  日本学術会議　総合工学委員会ICO分科会, 第二回先端フォトニクスシンポジウム人気ポスター賞
• 05 Mar. 2012

  （公財）コニカミノルタ科学技術振興財団, コニカミノルタ画像科学奨励賞
• 31 May 2012

  （一社）レーザー学会, レーザー学会第32回年次大会優秀論文発表賞
• 20 Feb. 2013

  （公財）光科学技術研究振興財団, 平成24年度研究表彰
• 31 Aug. 2016

  (国研) 理化学研究所4D細胞計測プロジェクト　, 第3回4D細胞計測若手の会ベストプレゼンテーション賞
• 01 Nov. 2016

  (国研) 理化学研究所 光量子工学研究領域, 第4回理研シンポジウム「光量子工学研究」The Best Poster Award
• 17 Apr. 2018

  文部科学省, 文部科学大臣表彰，若手科学者賞