Basic Information

Faculty

• Faculty of Agriculture

Email Address

• Email Address

  fujihashi.masahiro.6ckyoto-u.ac.jp

Academic Degree

• 23 Mar. 1998

  京都大学修士（理学）
• 23 Mar. 2001

  京都大学博士（理学）

Academic Resume (Graduate Schools)

• 京都大学, 大学院理学研究科博士後期課程化学専攻, 修了
• 京都大学, 大学院理学研究科修士課程化学専攻, 修了

Academic Resume (Undergraduate School/Majors)

• 京都大学, 理学部, 卒業

ID,URL

• J-Global ID

  201401038787297205

researchmap URL

• https://researchmap.jp/fujihashi_masahiro

Research

Research Areas

• Life sciences, Structural biochemistry

Papers

• AlphaFold2 Insights into c-Abl Transactivation by Adaptor c-Crk or Abi-1: Structural Forms, Active Site Types, and Multiple SH3-PxxPxK/R Interactions

  Tomoyuki Shishido; Masahiro Fujihashi

  Genes to Cells, 31 Jan. 2026, Last author

  
• Expanding the Structural Diversity of Sesquarterpenes through Genome Mining of Noncanonical Class IB Terpene Synthases.

  Shogo Iwakata; Ikiru Otsuka; Shuya Azuma; Yuka Takaba; Saya Sugisawa; Yumika Suzuki; Yu Shinohara; Yurie Fujii; Daijiro Ueda; Tohru Taniguchi; Masahiro Fujihashi; Tetsuro Shinada; Tsutomu Sato

  ACS chemical biology, 21 Dec. 2025

  
• Heat‐sterilizable antibody mimics designed on the cold shock protein scaffold from hyperthermophile Thermotoga maritima

  Hiroshi Amesaka; Marin Tachibana; Mizuho Hara; Shuntaro Toya; Haruki Nakagawa; Hiroyoshi Matsumura; Azumi Hirata; Masahiro Fujihashi; Kazufumi Takano; Shun‐ichi Tanaka

  Protein Science, 26 Dec. 2024

  
• Investigation of Enzyme Reaction Mechanism, and Exploration and Construction of New Enzymes Based on Crystal Structures

  Masahiro FUJIHASHI

  Nihon Kessho Gakkaishi, 31 May 2024, Lead author, Corresponding author

  
• Identification and functional/structural analyses of large terpene synthases

  Daijiro Ueda; Tohru Abe; Masahiro Fujihashi; Tsutomu Sato

  Methods in Enzymology, 2024, Peer-reviewed, Invited, Corresponding author

  
• Insight into the mechanism of geranyl-β-phellandrene formation catalyzed by Class IB terpene synthases.

  Shogo Iwakata; Kazuya Asada; Tomoyuki Nishi; Rafaella Stepanova; So Shinoda; Daijiro Ueda; Masahiro Fujihashi; Yoko Yasuno; Tetsuro Shinada; Tsutomu Sato

  Bioscience, biotechnology, and biochemistry, 24 May 2022

  
• Identification and enzymatic analysis of an archaeal ATP-dependent serine kinase from the hyperthermophilic archaeon Staphylothermus marinus

  Yasunobu Mori; Hiroki Kawamura; Takaaki Sato; Takayuki Fujita; Ryuhei Nagata; Masahiro Fujihashi; Kunio Miki; Haruyuki Atomi

  Journal of Bacteriology, 07 Jun. 2021

  
• Altering the Phosphorylation Position of Pyrophosphate-Dependent myo-Inositol-1-Kinase Based on Its Crystal Structure

  Ryo Tashiro; Takaaki Sato; Haruyuki Atomi; Kunio Miki; Masahiro Fujihashi

  ACS Chemical Biology, 21 May 2021, Peer-reviewed, Last author, Corresponding author

  
• Characterization of Class IB Terpene Synthase: The First Crystal Structure Bound with a Substrate Surrogate.

  Rafaella Stepanova; Hayato Inagi; Kei Sugawara; Kazuya Asada; Tomoyuki Nishi; Daijiro Ueda; Yoko Yasuno; Tetsuro Shinada; Kunio Miki; Masahiro Fujihashi; Tsutomu Sato

  ACS chemical biology, 19 Jun. 2020, Peer-reviewed

  Access this paper :

  
• Identification of a pyrophosphate-dependent kinase and its donor selectivity determinants

  Ryuhei Nagata; Masahiro Fujihashi; Takaaki Sato; Haruyuki Atomi; Kunio Miki

  Nature Communications, 01 Dec. 2018, Peer-reviewed

  Access this paper :

  
• Investigation of the Reaction Mechanism of Prenyl-Chain Transformation Enzymes

  Fujihashi Masahiro; Mito Kazuya; Iwata Yuki; Hirose Kana; Watanabe Bunta; Hemmi Hisashi; Miki Kunio

  SPring-8/SACLA Research Report, 2018

  
• Crystal structure and functional analysis of large-terpene synthases belonging to a newly found subclass

  Masahiro Fujihashi; Tsutomu Sato; Yuma Tanaka; Daisuke Yamamoto; Tomoyuki Nishi; Daijiro Ueda; Mizuki Murakami; Yoko Yasuno; Ai Sekihara; Kazuma Fuku; Tetsuro Shinada; Kunio Miki

  Chemical Science, 2018, Peer-reviewed

  Access this paper :

  
• タンパク質の分子構造変化を捉える - X 線自由電子レーザーによる連続スナップショット -

  藤橋 雅宏

  化学, Aug. 2017, Invited

  
• Structural Study on the Reaction Mechanism of a Free Serine Kinase Involved in Cysteine Biosynthesis

  Ryuhei Nagata; Masahiro Fujihashi; Hiroki Kawamura; Takaaki Sato; Takayuki Fujita; Haruyuki Atomi; Kunio Miki

  ACS CHEMICAL BIOLOGY, Jun. 2017, Peer-reviewed

  
• Mutation design of a thermophilic Rubisco based on three-dimensional structure enhances its activity at ambient temperature

  Masahiro Fujihashi; Yuichi Nishitani; Tomohiro Kiriyama; Riku Aono; Takaaki Sato; Tomoyuki Takai; Kenta Tagashira; Wakao Fukuda; Haruyuki Atomi; Tadayuki Imanaka; Kunio Miki

  PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, Oct. 2016, Peer-reviewed

  
• Crystal Structure and Product Analysis of an Archaeal myo-Inositol Kinase Reveal Substrate Recognition Mode and 3-OH Phosphorylation

  Ryuhei Nagata; Masahiro Fujihashi; Takaaki Sato; Haruyuki Atomi; Kunio Miki

  BIOCHEMISTRY, Jun. 2015, Peer-reviewed

  
• Orotidine Monophosphate Decarboxylase - A Fascinating Workhorse Enzyme with Therapeutic Potential

  Masahiro Fujihashi; Jagjeet S. Mnpotra; Ram Kumar Mishra; Emil F. Pai; Lakshmi P. Kotra

  JOURNAL OF GENETICS AND GENOMICS, May 2015, Peer-reviewed

  
• Orotidine Monophosphate Decarboxylase - A Fascinating Workhorse Enzyme with Therapeutic Potential

  Masahiro Fujihashi; Jagjeet S. Mnpotra; Ram Kumar Mishra; Emil F. Pai; Lakshmi P. Kotra

  JOURNAL OF GENETICS AND GENOMICS, May 2015, Peer-reviewed, Invited

  
• Structural characterization of a ligand-bound form of Bacillus subtilis FadR involved in the regulation of fatty acid degradation

  Masahiro Fujihashi; Taiga Nakatani; Kazutake Hirooka; Hiroshi Matsuoka; Yasutaro Fujita; Kunio Miki

  PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, Jul. 2014, Peer-reviewed

  
• 結晶構造を基にした酵素反応機構の解析 ~オロチジン一リン酸脱炭酸酵素を題材に~

  藤橋雅宏

  日本結晶学会誌, 2014

  
• Substrate Distortion Contributes to the Catalysis of Orotidine 5 '-Monophosphate Decarboxylase

  Masahiro Fujihashi; Toyokazu Ishida; Shingo Kuroda; Lakshmi P. Kotra; Emil F. Pai; Kunio Miki

  JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Nov. 2013, Peer-reviewed

  
• An Uncharacterized Member of the Ribokinase Family in Thermococcus kodakarensis Exhibits myo-Inositol Kinase Activity

  Takaaki Sato; Masahiro Fujihashi; Yukika Miyamoto; Keiko Kuwata; Eriko Kusaka; Haruo Fujita; Kunio Miki; Haruyuki Atomi

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jul. 2013, Peer-reviewed

  
• Atomic resolution structure of the orotidine 5′-monophosphate decarboxylase product complex combined with surface plasmon resonance analysis: Implications for the catalytic mechanism

  Masahiro Fujihashi; Kazuya Mito; Emil F. Pai; Kunio Miki

  Journal of Biological Chemistry, 29 Mar. 2013, Peer-reviewed

  
• Crystal sample pins and a storage cassette system compatible with the protein crystallography beamlines at both the Photon Factory and SPring-8

  Masahiro Fujihashi; Masahiko Hiraki; Go Ueno; Seiki Baba; Hironori Murakami; Mamoru Suzuki; Nobuhisa Watanabe; Isao Tanaka; Atsushi Nakagawa; Soichi Wakatsuki; Masaki Yamamoto; Kunio Miki

  JOURNAL OF APPLIED CRYSTALLOGRAPHY, Dec. 2012, Peer-reviewed

  
• Dynamic, Ligand-dependent Conformational Change Triggers Reaction of Ribose-1,5-bisphosphate Isomerase from Thermococcus kodakarensis KOD1

  Akira Nakamura; Masahiro Fujihashi; Riku Aono; Takaaki Sato; Yosuke Nishiba; Shosuke Yoshida; Ayumu Yano; Haruyuki Atomi; Tadayuki Imanaka; Kunio Miki

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jun. 2012, Peer-reviewed

  
• Structure and Mutation Analysis of Archaeal Geranylgeranyl Reductase

  Daisuke Sasaki; Masahiro Fujihashi; Yuki Iwata; Motomichi Murakami; Tohru Yoshimura; Hisashi Hemmi; Kunio Miki

  JOURNAL OF MOLECULAR BIOLOGY, Jun. 2011, Peer-reviewed

  
• Crystal Structure of Heterodimeric Hexaprenyl Diphosphate Synthase from Micrococcus luteus B-P 26 Reveals That the Small Subunit Is Directly Involved in the Product Chain Length Regulation

  Daisuke Sasaki; Masahiro Fujihashi; Naomi Okuyama; Yukiko Kobayashi; Motoyoshi Noike; Tanetoshi Koyama; Kunio Miki

  JOURNAL OF BIOLOGICAL CHEMISTRY, Feb. 2011, Peer-reviewed

  
• Structure-based Catalytic Optimization of a Type III Rubisco from a Hyperthermophile

  Yuichi Nishitani; Shosuke Yoshida; Masahiro Fujihashi; Kazuya Kitagawa; Takashi Doi; Haruyuki Atomi; Tadayuki Imanaka; Kunio Miki

  JOURNAL OF BIOLOGICAL CHEMISTRY, Dec. 2010, Peer-reviewed

  
• Crystal Structure and the Reaction Mechanism of cis-prenyltransferase

  FUJIHASHI Masahiro

  X-RAYS, 30 Oct. 2009, Peer-reviewed

  
• [Development of automatic crystal mounting system commonly utilizable for SPring-8 and photon factory].

  Fujihashi M; Miki K

  Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, Sep. 2009, Peer-reviewed

  
• Structural Characterization of the Molecular Events during a Slow Substrate-Product Transition in Orotidine 5 '-Monophosphate Decarboxylase

  Masahiro Fujihashi; Lianhu Wei; Lakshmi P. Kotra; Emil F. Pai

  JOURNAL OF MOLECULAR BIOLOGY, Apr. 2009, Peer-reviewed

  
• 2P-008 Structure-based optimization for catalytic activity of a Type III Rubisco from a hyperthermophile(Protein:Structure,The 47th Annual Meeting of the Biophysical Society of Japan)

  Nishitani Yuichi; Fujihashi Masahiro; Doi Takashi; Yoshida Shosuke; Atomi Haruyuki; Imanaka Tadayuki; Miki Kunio

  Seibutsu Butsuri, 2009

  
• Structural and Molecular Characterization of the Prefoldin beta Subunit from Thermococcus Strain KS-1

  Hiroshi Kida; Yuri Sugano; Ryo Iizuka; Masahiro Fujihashi; Masafurni Yohda; Kunio Miki

  JOURNAL OF MOLECULAR BIOLOGY, Nov. 2008, Peer-reviewed

  
• Crystal structure of human cyclophilin D in complex with its inhibitor, cyclosporin A at 0.96-angstrom resolution

  Kenji Kajitani; Masahiro Fujihashi; Yukiko Kobayashi; Shigeomi Shimizu; Yoshihide Tsujimoto; Kunio Miki

  PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, Mar. 2008, Peer-reviewed

  
• 1S6-5 Efficient Crystal Screening and Application of Crystal Mounting Robots(1S6 Cutting edge of protein crystallography with synchrotron radiation,The 46th Annual Meeting of the Biophysical Society of Japan)

  Fujihashi Masahiro

  Seibutsu Butsuri, 2008

  
• 1P-025 Structural investigation of orotidme monophosphate decarboxylase : the role of the catalytic lysine(The 46th Annual Meeting of the Biophysical Society of Japan)

  Kuroda Shingo; Fujihashi Masahiro; Lakshmi P. Kotra; Pai Emil F.; Miki Kunio

  Seibutsu Butsuri, 2008

  
• Structural and molecular characterization of the prefoldin beta subunit from Thermococcus strain

  Kida Hiroshi; Sugano Yuri; Iizuka Ryo; Fujihashi Masahiro; Yohda Masafumi; Miki Kunio

  ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2008, Peer-reviewed

  
• A potent, covalent inhibitor of orotidine 5 '-monophosphate decarboxylase with antimalarial activity

  Angelica M. Bello; Ewa Poduch; Masahiro Fujihashi; Merhnaz Amani; Yan Li; Ian Crandall; Raymond Hui; Ping I. Lee; Kevin C. Kain; Emil F. Pai; Lakshmi P. Kotra

  JOURNAL OF MEDICINAL CHEMISTRY, Mar. 2007, Peer-reviewed

  
• Crystal structure of archaeal photolyase from Sulfolobus tokodaii with two FAD molecules: Implication of a novel light-harvesting cofactor

  Masahiro Fujihashi; Nobutaka Numoto; Yukiko Kobayashi; Akira Mizushima; Masanari Tsujimura; Akira Nakamura; Yutaka Kawarabayasi; Kunio Miki

  JOURNAL OF MOLECULAR BIOLOGY, Jan. 2007, Peer-reviewed

  
• Erratum: A potent, covalent inhibitor of orotidine 5′-monophosphate decarboxylase with antimalarial activity (Journal of Medicinal Chemistry (2007) 50, (965))

  A.M. Bello; E. Poduch; M. Fujihashi; M. Amani; Y. Li; I. Crandall; R. Hui; P.I. Lee; K.C. Kain; E.F. Pai; L.P. Kotra

  Journal of Medicinal Chemistry, 2007, Peer-reviewed

  
• 2P020 Crystal Structure of Prefoldin beta Subunits Oligomer(Proteins-structure and structure-function relationship,Poster Presentations)

  Kida Hiroshi; Sugano Yuri; Iizuka Ryo; Fujihashi Masahiro; Yohda Masafumi; Miki Kunio

  Seibutsu Butsuri, 2007

  
• Design of inhibitors of orotidine monophosphate decarboxylase using bioisosteric replacement and determination of inhibition kinetics

  Ewa Poduch; Angelica M. Bello; Sishi Tang; Masahiro Fujihashi; Emil F. Pai; Lakshmi P. Kotra

  JOURNAL OF MEDICINAL CHEMISTRY, Aug. 2006, Peer-reviewed

  
• The crystal structure of (S)-3-O-geranylgeranylglyceryl phosphate synthase reveals an ancient fold for an ancient enzyme

  J Payandeh; M Fujihashi; W Gillon; EF Pai

  JOURNAL OF BIOLOGICAL CHEMISTRY, Mar. 2006, Peer-reviewed

  
• An unprecedented twist to ODCase catalytic activity

  M Fujihashi; AM Bello; E Poduch; LH Wei; SC Annedi; EF Pai; LP Kotra

  JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Nov. 2005, Peer-reviewed

  
• タンパク質結晶学の位相決定法における新たな展開

  藤橋雅宏; 三木邦夫

  化学, 01 May 2005, Peer-reviewed

  
• The crystal structure of an oxidatively stable subtilisin-like alkaline serine protease, KP-43, with a C-terminal beta-barrel domain

  T Nonaka; M Fujihashi; A Kita; K Saeki; S Ito; K Horikoshi; K Miki

  JOURNAL OF BIOLOGICAL CHEMISTRY, Nov. 2004, Peer-reviewed

  
• Involvement of tyrosines at fucose-binding sites of Aleuria aurantia lectin: Non-equal response to site-directed mutagenesis among five sites

  K Amano; M Fujihashi; A Ando; K Miki; Y Nagata

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Apr. 2004, Peer-reviewed

  
• 1P023 Crystal structure of KP-43 protease and alteration of substrate specificity of subtilisin

  Nonaka T.; Fujihashi M.; Kita A.; Saeki K.; Ito S.; Horikoshi K.; Miki K.

  Seibutsu Butsuri, 2004

  
• Significance of highly conserved aromatic residues in Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase

  Y Khare; YW Zhang; M Fujihashi; K Miki; T Koyama

  JOURNAL OF BIOCHEMISTRY, Dec. 2003, Peer-reviewed

  
• Crystal structure of fucose-specific lectin from Aleuria aurantia binding ligands at three of its five sugar recognition sites

  M Fujihashi; DH Peapus; N Kamiya; Y Nagata; K Miki

  BIOCHEMISTRY, Sep. 2003, Peer-reviewed

  
• Crystal structure of calcium-free alpha-amylase from Bacillus sp strain KSM-K38 (AmyK38) and its sodium ion binding sites

  T Nonaka; M Fujihashi; A Kita; H Hagihara; K Ozaki; S Ito; K Miki

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jul. 2003, Peer-reviewed

  
• Mutational analysis of allylic substrate binding site of Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase

  K Fujikura; YW Zhang; M Fujihashi; K Miki; T Koyama

  BIOCHEMISTRY, Apr. 2003, Peer-reviewed

  
• X-ray crystallographic characterization and phasing of a fucose-specific lectin from Aleuria aurantia

  M Fujihashi; DH Peapus; E Nakajima; T Yamada; J Saito; A Kita; Y Higuchi; Y Sugawara; A Ando; N Kamiya; Y Nagata; K Miki

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Feb. 2003, Peer-reviewed

  
• Crystal structures of the oxidatively stable alkaline serine protease (KP-43 protease) and oxidative inactivation mechanism of subtilisins

  Nonaka T.; Fujihashi M.; Kita A.; Saeki K.; Horikoshi K.; Ito S.; Miki K.

  Seibutsu Butsuri, 2003

  
• Identification of Significant Residues for Homoallylic Substrate Binding of Micrococcus luteus B-P 26 Undecaprenyl Diphosphate Synthase

  Yugesh Kharel; Yuan-Wei Zhang; Masahiro Fujihashi; Kunio Miki; Tanetoshi Koyama

  Journal of Biological Chemistry, 27 Jul. 2001, Peer-reviewed

  
• Crystallization and preliminary X-ray diffraction studies of a novel alkaline serine protease (KP-43) from alkaliphilic Bacillus sp strain KSM-KP43

  T Nonaka; M Fujihashi; A Kita; K Saeki; S Ito; K Miki

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, May 2001, Peer-reviewed

  
• Crystal structure of cis-prenyl chain elongating enzyme, undecaprenyl diphosphate synthase

  M Fujihashi; YW Zhang; Y Higuchi; XY Li; T Koyama; K Miki

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Apr. 2001, Peer-reviewed

  
• 35 Structure and Function of cis-Prenyl Chain Elongating Enzyme : Structure analysis of Micrococcus luteus B-P 26 Undecaprenyl Diphosphate Synthase

  Koyama Tanetoshi; Shimizu Naoto; Zhang Yuan-Wei; Ogura Kyozo; Fujihashi Masahiro; Miki Kunio

  Symposium on the Chemistry of Natural Products, symposium papers, 01 Oct. 2000

  
• Crystallization and preliminary X-ray diffraction studies of undecaprenyl diphosphate synthase from Micrococcus luteus B-P 26

  M Fujihashi; N Shimizu; YW Zhang; T Koyama; K Miki

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Sep. 1999, Peer-reviewed

  
• 大腸菌の転写終結因子Rhoの結晶化

  藤橋 雅宏; 堀口 大吾; 重定 勝哉; 三木 邦夫

  日本結晶学会誌, 1997

  

Misc.

• Genome mining of Class IB terpene synthases

  吾妻秀哉; 大塚生; 藤橋雅宏; 品田哲郎; 上田大次郎; 佐藤努

  香料・テルペンおよび精油化学に関する討論会講演要旨集, 2024

  
• Crystallographic Analysis with Anomalous Dispersion Effects of Phosphorus Atoms Using Remote and Automated Measurements

  Masahiro FUJIHASHI

  Nihon Kessho Gakkaishi, 31 Aug. 2021, Peer-reviewed, Invited, Lead author, Corresponding author

  
• Analysis of cyclic structure formation mechanism of class IB terpene synthase

  浅田和也; 上田大次郎; 三木邦夫; 藤橋雅宏; 品田哲郎; 佐藤努

  日本農芸化学会大会講演要旨集(Web), 2021

  
• クラスIB大型テルペン合成酵素の基質複合体の結晶構造

  金本壮平; 佐藤努; 品田哲朗; 三木邦夫; 深井周也; 藤橋雅宏

  日本結晶学会年会講演要旨集, 2020

  
• Class IB大型テルペン合成酵素の基質認識機構の解析

  藤橋雅宏; 稲木隼人; 西智之; 上田大次郎; 佐藤努; 品田哲朗; 三木邦夫

  イソプレノイド研究会例会講演要旨集, 2019

  
• Analysis of ligand-recognition mechanism of class IB terpene synthase

  稲木隼人; 佐藤努; 保野陽子; 品田哲郎; 三木邦夫; 藤橋雅宏

  量子ビームサイエンスフェスタ(Web), 2019

  
• クラスIBテルペン合成酵素の触媒反応の解析

  西智之; STEPANOVA Rafaella; 菅原啓; 上田大次郎; 藤橋雅宏; 三木邦夫; 保野陽子; 品田哲郎; 佐藤努

  日本農芸化学会大会講演要旨集(Web), 2019

  
• ATPでなくピロリン酸を利用する新規リン酸化酵素の基質認識機構

  永田隆平; 藤橋雅宏

  化学と生物, Nov. 2018, Peer-reviewed

  
• 新規ファミリーに属するテルペン合成酵素の基質認識部位の同定

  稲木隼人; 佐藤努; 保野陽子; 品田哲郎; 三木邦夫; 藤橋雅宏

  日本結晶学会年会講演要旨集, 01 Nov. 2018

  
• Large‐terpene合成酵素の酵素的諸性質の解析と部位特異的変異

  西智之; 菅原啓; 小川佳央; 高橋宏忠; 上田大次郎; 藤橋雅宏; 三木邦夫; 保野陽子; 品田哲郎; 佐藤努

  日本蛋白質科学会年会プログラム・要旨集, 23 May 2018

  
• Large‐terpene合成酵素の酵素的諸性質の解析と部位特異的変異

  菅原啓; 西智之; 小川佳央; 高橋宏忠; 上田大次郎; 藤橋雅宏; 三木邦夫; 保野陽子; 品田哲郎; 佐藤努

  日本農芸化学会大会講演要旨集(Web), 05 Mar. 2018

  
• Large‐terpene合成酵素の結晶構造解析

  藤橋雅宏; 佐藤努; 田中勇真; 山本大輔; 西智之; 上田大次郎; 村上瑞気; 保野陽子; 関原あい; 福和真; 品田哲郎; 三木邦夫

  日本農芸化学会大会講演要旨集(Web), 05 Mar. 2018

  
• Large-terpene合成酵素の構造と機能

  藤橋雅宏; 佐藤努; 田中勇真; 山本大輔; 西智之; 上田大次郎; 村上瑞気; 保野陽子; 関原あい; 福和真; 品田哲朗; 三木邦夫

  日本蛋白質科学会年会プログラム・要旨集, 2018

  
• Large-terpene合成酵素の酵素学的諸性質の解析と部位特異的変異

  西智之; 菅原啓; 小川佳央; 高橋宏忠; 上田大次郎; 藤橋雅宏; 三木邦夫; 保野陽子; 品田哲郎; 佐藤努

  香料・テルペンおよび精油化学に関する討論会講演要旨集, 2018

  
• Large-terpene合成酵素の構造機能相関

  藤橋雅宏; 佐藤努; 田中勇真; 山本大輔; 西智之; 上田大次郎; 村上瑞気; 保野陽子; 関原あい; 福和真; 品田哲朗; 三木邦夫

  イソプレノイド研究会例会講演要旨集, 2018

  
• Bacillus属細菌由来テルペノイドのユニークな生合成経路の解析

  上田大次郎; 松ヶ根沙織; 西智之; 菅原啓; 岡本渉; 藤橋雅宏; 三木邦夫; 橋本昌征; 保野陽子; 品田哲郎; 佐藤努

  イソプレノイド研究会例会講演要旨集, 2018

  
• Moorella thermoacetica由来テトラプレニル‐β‐クルクメン合成酵素ホモログのゲノムマイニング

  藤井友里恵; 田中勇真; 藤橋雅宏; 三木邦夫; 河合優人; 中島田豊; 品田哲郎; 佐藤努

  日本農芸化学会大会講演要旨集(Web), 05 Mar. 2016

  
• 超好熱性アーキアThermococcus kodakarensisにおけるribokinase familyタンパク質の機能解明

  佐藤喬章; 青野陸; 藤橋雅宏; 宮本幸花; 桑田啓子; 日下絵里子; 藤田春雄; 三木邦夫; 今中忠行; 跡見晴幸

  日本生物工学会大会講演要旨集, 05 Aug. 2014

  
• 立体構造に基づくイノシトールリン酸化酵素の基質認識機構と生成物の解明

  NAGATA RYUHEI; FUJIHASHI MASAHIRO; SATO TAKAAKI; SATO TAKAAKI; ATOMI HARUYUKI; ATOMI HARUYUKI; MIKI KUNIO; MIKI KUNIO

  日本生化学会大会(Web), 2014

  
• プレニル鎖の伸長や修飾に関わる酵素の構造機能解析

  藤橋雅宏; 佐々木大輔; 三木邦夫

  日本農芸化学会2013年度大会, 東北大学川内北キャンパス (仙台市), 4SY28-4, 2013, Peer-reviewed

  
• Fully Automated Data Collection Using PAM and the Development of PAM/SPACE Reversible Cassettes

  Masahiko Hiraki; Shokei Watanabe; Leonard M. G. Chavas; Yusuke Yamada; Naohiro Matsugaki; Noriyuki Igarashi; Soichi Wakatsuki; Masahiro Fujihashi; Kunio Miki; Seiki Baba; Go Ueno; Masaki Yamamoto; Mamoru Suzuki; Atsushi Nakagawa; Nobuhisa Watanabe; Isao Tanaka

  SRI 2009: THE 10TH INTERNATIONAL CONFERENCE ON SYNCHROTRON RADIATION INSTRUMENTATION, 2010, Peer-reviewed

  
• Molecular events of a slow substrate-product transition in orotidine 5'-monophosphate decarboxylase

  Masahiro Fujihashi; Angelica M. Bello; Lakshmi P. Kotra; F. Pai

  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Mar. 2009

  
• 結晶の効率的なスクリーニングとロボットの利用

  藤橋雅宏

  日本生物物理学会第46回年会, 福岡国際会議場(福岡市), 1S6-5,, 2008, Peer-reviewed

  
• A potent, covalent inhibitor of orotidine 5 '-monophosphate decarboxylase with antimalarial activity (vol 50, pg 915, 2007)

  Angelica M. Bello; Ewa Poduch; Masahiro Fujihashi; Merhnaz Amani; Yan Li; Ian Crandall; Raymond Hui; Ping I. Lee; Kevin C. Kain; Emil F. Pai; Lakshmi P. Kotra

  JOURNAL OF MEDICINAL CHEMISTRY, May 2007

  
• Characterization of novel inhibitors of orotidine 5 '-monophosphate decarboxylase (ODCase) using isothermal calorimetry

  Ewa Poduch; Angelica M. Bello; Masahiro Fujihashi; Emil F. Pai; Lakshmi P. Kotra

  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Mar. 2007

  
• Covalent inhibitor of orotidine 5 '-monophosphate decarboxylase with antimalarial activity

  Angelica M. Bello; Ewa Poduch; Masahiro Fujihashi; Yan Li; Ian Crandall; Ping I. Lee; Kevin C. Kain; Emil F. Pai; Lakshmi P. Kotra

  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Mar. 2007

  
• Orotidine monophosphate decarboxylase: Novel inhibitors with new twists and turns

  Lakshmi P. Kotra; Emil F. Pai; Angelica M. Bello; Masahiro Fujihashi; Ewa Poduch

  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Mar. 2006

  
• 2P012 Crystallographic study of Paa proteins involved in catabolism of phenylacetic acid

  Kida H; Iino H; Nakagawa N; Hisano T; Fujihashi M; Kita A; Miki K

  Biophysics, 19 Oct. 2005

  
• The crystal structure of a novel subtilisin-like alkaline serine protease、 KP-43.

  T. Nonaka; M. Fujihashi; A. Kita; K. Saeki; S. Ito; K. Hirikoshi; K. Miki

  XX congress of the International Union of Crystallography、 Congress and General Assembly, 2005

  
• Combining chemistry, molecular and structural biology in search of the elusive mechanism of orotidine monophosphate decarboxylase.

  EF Pai; M Fujihashi; N Wu; LH Wei; AM Bello; SC Annedi; LP Kotra

  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Aug. 2004

  
• Crystal Structures of Alkaline α-Amylase、 Amyk38 and Its Complexes with Oligosaccharides.

  T. Nonaka; M. Fujihashi; A. Kita; H. Hagihara; K. Ozaki; S. Ito; K. Miki

  The 1st Pasific-Rim International Conference on Protein Science and the 4th Annual Meeting of the Protein Science Society of Japan, 2004

  
• X-ray crystal structure analysis of a novel type of a-amylase

  T. Nonaka; M. Fujihashi; A. Kita; K. Miki

  SPring-8 User Experimental Report No.8 (2001B), 2002

  
• Crystal Structure of a Calcium-free α-Amylase AMYK38

  T. Nonaka; M. Fujihashi; A. Kita; H. Hagihara; K. Ozaki; S. Ito; K. Miki

  XIXth Congress and General Assembly、 International Union of Crystallography\n, 2002

  

Books and Other Publications

• 日本の結晶学II

  藤橋 雅宏, Contributor, プレニル鎖伸長酵素の構造と鎖長制御機構

  日本結晶学会, 2014, Not refereed

  

Awards

• 27 Oct. 2023

  学術賞（日本結晶学会）
• 28 Nov. 2023

  令和4年度ベストティーチャー賞（大阪医科薬科大学）
• 27 Aug. 2008

  平成２０年度日本結晶学会賞･進歩賞

External funds: Kakenhi

• Visualization of protonated states of dissociative substituents by cooperation of quantum calculations and crystal structures

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields

  Osaka Medical and Pharmaceutical University

  藤橋 雅宏

  From 30 Jun. 2022, To 31 Mar. 2026, Granted

  結晶構造解析;結晶構造;量子計算

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K19278/
• Mass identification and modification of pyrophosphate-dependent kinases based on their three-dimensional structures

  Grant-in-Aid for Scientific Research (B)

  Basic Section 43020:Structural biochemistry-related

  Osaka Medical and Pharmaceutical University

  Masahiro Fujihashi

  From 01 Apr. 2021, To 31 Mar. 2024, Project Closed

  結晶構造解析;ピロリン酸;結晶構造;リン酸化酵素;ピロリン酸依存性リン酸化酵素;無細胞タンパク質合成

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H02413/
• High-Resolution Crystal Structure Analyses of Electron-Transfer Proteins by Combined and Cooperative Use of Synchrotron X-ray and Neutron Diffraction

  Grant-in-Aid for Scientific Research (C)

  Basic Section 43020:Structural biochemistry-related

  Kyoto University

  Kunio Miki

  From 01 Apr. 2020, To 31 Mar. 2025, Project Closed

  電子伝達タンパク質;精密構造解析;中性子線回折;放射光Ｘ線回折;電荷密度解析

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K06523/
• multiple identification of non-ATP-dependent kinases based on registered crystal structures of enzymes with unknown function

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields

  Osaka Medical and Pharmaceutical University;Kyoto University

  Masahiro Fujihashi

  From 28 Jun. 2019, To 31 Mar. 2022, Project Closed

  ピロリン酸;結晶構造;立体構造;リン酸基;リン酸化酵素;リン酸化;キナーゼ;結晶構造解析

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K22385/
• Research on Mining, functional analysis, and modification of biosynthetic enzymes based on three-dimensional structure

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Science and Engineering

  Kyoto University

  藤橋 雅宏

  From 01 Apr. 2019, To 31 Mar. 2021, Project Closed

  結晶構造解析;酵素;ピロリン酸;テルペン;立体構造

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-19H04652/
• Discovery of new terpene cyclization pathway and identification of various novel terpenes

  Grant-in-Aid for Scientific Research (B)

  Basic Section 38040:Bioorganic chemistry-related

  Niigata University

  Tsutomu Sato

  From 01 Apr. 2018, To 31 Mar. 2021, Project Closed

  生合成;テルペン;イソプレノイド;テルペノイド;酵素

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18H02145/
• 新規酵素の立体構造を基にした、有用酵素のマイニングと機能改良

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Science and Engineering

  Kyoto University

  藤橋 雅宏

  From 01 Apr. 2017, To 31 Mar. 2019, Project Closed

  結晶構造解析;テルペン合成酵素;ピロリン酸;リン酸化酵素;酵素反応;構造生物学;タンパク質

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-17H05439/
• Structural Biology of Molecular Mechanism of Hydrogenase Maturation Proteins

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Kunio Miki

  From 01 Apr. 2017, To 31 Mar. 2020, Project Closed

  Ｘ線結晶解析;ヒドロゲナーゼ;タンパク質の成熟化;Hyp成熟化タンパク質;構造機能相関;構造生物学;成熟化タンパク質;X線結晶解析

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17H03642/
• Experimental analysis of the electrostatic repulsion between enzyme and substrates to elucidate the reaction mechanism, and development anti-malaria agent

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Masahiro Fujihashi

  From 01 Apr. 2016, To 31 Mar. 2019, Project Closed

  酵素;X線結晶構造解析;結晶構造;プロトン化状態;酵素反応;構造生物学;タンパク質;結晶構造解析;基質の歪み;オロチジン一リン酸脱炭酸酵素

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16H04751/
• Creation of novel terpenoids from biosynthetic study on sesquarterpene

  Grant-in-Aid for Scientific Research (C)

  Niigata University

  Tsutomu Sato

  From 01 Apr. 2013, To 31 Mar. 2016, Project Closed

  テルペン;テルペノイド;イソプレノイド;生合成

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25450149/
• Elucidation of catalytic mechanism of two characteristic enzymes and its application based on crystal structures.

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Masahiro FUJIHASHI

  From 01 Apr. 2012, To 31 Mar. 2015, Project Closed

  基質の歪み;脱炭酸反応;結晶構造解析;高分解能結晶構造;計算機シミュレーション;酵素反応機構;ODCase;OMPDC;OMPDCase;X線結晶構造;国際情報交換;X線結晶構造解析;酵素反応;結晶構造

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24570130/
• Investigation of the reaction mechanism of orotidine monophosphate decarboxylase using the distortion of the substrate

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  Masahiro FUJIHASHI

  From 01 Apr. 2010, To 31 Mar. 2012, Project Closed

  X線結晶解析;ODCase;反応中間体構造;基質構造の歪み;酵素反応機構;蛋白質X線結晶構造解析;酵素基質複合体;OMPDC;OMPDCase;タンパク質X線結晶構造解析

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22770102/
• Investigation of the structural change of orotidine 5'-monophosphate decarboxylase during the reaction and its catalytic mechanism

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  Masahiro FUJIHASHI

  From 01 Apr. 2008, To 31 Mar. 2010, Project Closed

  酵素;触媒反応機構;X線結晶構造解析;基質構造の歪み;ODCase;OMPDC;OMPDCase;反応中間体構造;反応機構;酵素基質複合体;ピリミジン新規合成

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20770081/
• 結晶構造の時間変化からみるオロチジン-リン酸脱炭酸酵素の反応触媒機構

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  藤橋 雅宏

  From 01 Apr. 2005, To 31 Mar. 2008, Project Closed

  ODCase;OMPDC;OMPDCase;X線結晶構造解析;反応機構;酵素基質複合体;副反応;ピリミジン新規合成;抗分解能X線構造;酵素反応;オロチジン-リン酸脱炭酸酵素;抗マラリア剤;抗ウイルス剤;結晶構造;高分解能X線構造;オロチジン一リン酸脱炭酸酵素;抗ウイルス薬

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17770087/
• Structure and Function of DNA Repair Enzyme and Their Homologous Proteins

  Grant-in-Aid for Scientific Research (A)

  Kyoto University

  Kunio MIKI

  From 01 Apr. 2002, To 31 Mar. 2005, Project Closed

  光回復酵素;クリプトクロム;青色光受容体;結晶化;X線結晶構造解析;BLUFドメイン;FAD;DNA修復;Cryptochrome;体内時計;CRY;X線回折実験;DNAグリコシラーゼ;塩基除去修復;Neil1;X線回析実験, Photolyase;Cryptochrome;Blue-Light Receptor;Crystallization;X-ray Crystallography;BLUE Domain;FAD;DNA Repair

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14208081/

Education

Teaching subject(s)

• From 01 Apr. 2026, To 31 Mar. 2027

  Introduction to Food Bioscience

  7329, Spring, Faculty of Agriculture, 1
• From 01 Apr. 2026, To 31 Mar. 2027

  Seminar on Biochemistry and Microbiology

  7317, Fall, Faculty of Agriculture, 1
• From 01 Apr. 2026, To 31 Mar. 2027

  Advanced Course of Protein Science

  GA35, Fall, Graduate School of Agriculture, 1
• From 01 Apr. 2026, To 31 Mar. 2027

  Food Science and Biotechnology VI

  GA19, Spring, Graduate School of Agriculture, 1
• From 01 Apr. 2026, To 31 Mar. 2027

  Enzyme Chemistry Seminar

  GF01, Spring, Graduate School of Agriculture, 1.5
• From 01 Apr. 2026, To 31 Mar. 2027

  Enzyme Chemistry Seminar

  GF01, Fall, Graduate School of Agriculture, 1.5
• From 01 Apr. 2026, To 31 Mar. 2027

  Laboratory Course in Food Biochemistry

  7325, Spring, Faculty of Agriculture, 3
• From 01 Apr. 2026, To 31 Mar. 2027

  Experimental Course of Enzyme Chemistry

  GG01, Spring, Graduate School of Agriculture, 3
• From 01 Apr. 2026, To 31 Mar. 2027

  Experimental Course of Enzyme Chemistry

  GG01, Fall, Graduate School of Agriculture, 3
• From 01 Apr. 2026, To 31 Mar. 2027

  Enzyme Chemistry

  7227, Fall, Faculty of Agriculture, 2
• From 01 Apr. 2026, To 31 Mar. 2027

  Physical Chemistry in Food Science II

  7219, Spring, Faculty of Agriculture, 2
• From 01 Apr. 2026, To 31 Mar. 2027

  Structural biology

  7331, Fall, Faculty of Agriculture, 2
• From 01 Apr. 2026, To 31 Mar. 2027

  Food Science and Production Technology

  7277, Spring, Faculty of Agriculture, 2
• From 01 Apr. 2025, To 31 Mar. 2026

  Advanced Course of Enzyme Chemistry

  GA22, Fall, Graduate School of Agriculture, 1
• From 01 Apr. 2025, To 31 Mar. 2026

  Introduction to Food Bioscience

  7329, Spring, Faculty of Agriculture, 1
• From 01 Apr. 2025, To 31 Mar. 2026

  Seminar on Biochemistry and Microbiology

  7317, Fall, Faculty of Agriculture, 1
• From 01 Apr. 2025, To 31 Mar. 2026

  Enzyme Chemistry Seminar

  GF01, Spring, Graduate School of Agriculture, 1.5
• From 01 Apr. 2025, To 31 Mar. 2026

  Enzyme Chemistry Seminar

  GF01, Fall, Graduate School of Agriculture, 1.5
• From 01 Apr. 2025, To 31 Mar. 2026

  Laboratory Course in Food Biochemistry

  7325, Spring, Faculty of Agriculture, 3
• From 01 Apr. 2025, To 31 Mar. 2026

  Experimental Course of Enzyme Chemistry

  GG01, Spring, Graduate School of Agriculture, 3
• From 01 Apr. 2025, To 31 Mar. 2026

  Experimental Course of Enzyme Chemistry

  GG01, Fall, Graduate School of Agriculture, 3
• From 01 Apr. 2025, To 31 Mar. 2026

  Structural biology

  7331, Fall, Faculty of Agriculture, 2
• From 01 Apr. 2025, To 31 Mar. 2026

  Fundamental Biochemistry I

  7301, Spring, Faculty of Agriculture, 2
• From 01 Apr. 2025, To 31 Mar. 2026

  Enzyme Chemistry

  7227, Fall, Faculty of Agriculture, 2

Participation in PhD Defense

• Studies on Structure-Function Relationship and Physiological Role of Bacterial Iron-Importing Efe System

  OKUMURA KENJI, Graduate School of Agriculture, Sub-chief Examiner

  23 Mar. 2026

Administration

Faculty management (title, position)

• From 01 Apr. 2026, To 31 Mar. 2027

  評価委員会　委員
• From 01 Apr. 2025, To 31 Mar. 2027

  研究科教務委員会　委員
• From 01 Apr. 2026, To 31 Mar. 2027

  食品生物科学科長