Basic Information

Affiliated programs (koza)

• Graduate School of Energy Science, エネルギー基礎科学専攻 エネルギー物質科学講座, 助教

Email Address

• Email Address

  arafiles.janvincent.7pkyoto-u.ac.jp

Professional Memberships

• From Apr. 2026, To Present

  Chemical Society of Japan
• From 2016, To Present

  Japanese Peptide Society
• From 2012, To Present

  Philippine Society for Biochemistry and Molecular Biology
• From 2022, To 2026

  European Peptide Society
• From 2016, To 2021

  Japanese Pharmeceutical Society

Academic Degree

• 27 Sep. 2017

  京都大学修士（薬学）
• 23 Sep. 2020

  京都大学博士（薬学）

Academic Resume (Undergraduate School/Majors)

• University of Santo Tomas, Faculty of Pharmacy, Department of Biochemistry, 卒業

Research History

• From Apr. 2026, To Present

  Kyoto University, Institute of Advanced Energy, Assistant Professor
• From Apr. 2021, To Mar. 2026

  Leibniz Forschungsinstitut für Molekulare Pharmakologie - Berlin, Biomolecule Modification and Delivery, Alexander von Humboldt Postdoctoral Fellow
• From Oct. 2020, To Mar. 2021

  Kyoto University, Institute for Chemical Research, Postdoctoral researcher

Language of Instruction

• Japanese
• English

ID,URL

• ORCID ID

  0000-0002-7733-7563

• J-Global ID

  202601003852051298

researchmap URL

• https://researchmap.jp/jvarafiles

Research

Research Interests

• Quantitative imaging
• Proteomics
• Photoproximity labeling
• Intracellular delivery
• Biomolecular organization
• Chemical Biology
• peptide
• liquid–liquid phase separation (LLPS)

Research Areas

• Nanotechnology/Materials, Nanobioscience
• Life sciences, Bioorganic chemistry
• Life sciences, Applied biochemistry

Papers

• Protein Modifications for Cellular Protein Delivery

  Jan Vincent V. Arafiles; Jonathan Franke; Palina Dubatouka; Luise Franz; Christian P.R. Hackenberger

  Chemical Reviews, 26 Aug. 2026

  
• Polyfluoralkylierte zellpenetrierende Peptid‐Additive verbessern die intrazelluläre Proteinaufnahme durch eine anhaltende und monomere Lipidinteraktion

  Sarah Hansen; Hana Zupan; Ferhat Mutlu; Jan Vincent V. Arafiles; Rubén Cruz; Palina Dubatouka; Kenichi Ataka; Martin Lehmann; Bettina G. Keller; Joachim Heberle; Christian P. R. Hackenberger

  Angewandte Chemie, 01 Jun. 2026

  
• Polyfluoroalkyl‐Tagged Cell‐Penetrating Peptide‐Additives Enhance Intracellular Protein Delivery via Sustained Monomeric Lipid Interaction

  Sarah Hansen; Hana Zupan; Ferhat Mutlu; Jan Vincent V. Arafiles; Rubén Cruz; Palina Dubatouka; Kenichi Ataka; Martin Lehmann; Bettina G. Keller; Joachim Heberle; Christian P. R. Hackenberger

  Angewandte Chemie International Edition, 01 Jun. 2026

  
• Cellular protein delivery through membrane potential driven water pores

  Jonathan Franke; Palina Dubatouka; Amin Yourdkhani; Sahil Soni; Tillmann Utesch; Juliana Serrano; Tolga Soykan; Martin Lehmann; Han Sun; Jan Vincent V. Arafiles; Christian P. R. Hackenberger

  25 Feb. 2026

  
• Modular Vinyl Phosphonamidates for Cysteine-Directed Protein Targeting

  Christian E. Stieger; Charlotte Völkel; Mathias B. Bertelsen; Michael Lisurek; Jan Vincent V. Arafiles; Jonathan Franke; Leander Crocker; Karl Schuppe; Yunjae Lim; Christiane Groneberg; Mathias Christmann; Han Sun; Christian P. R. Hackenberger

  Journal of the American Chemical Society, 25 Feb. 2026

  
• Active Site-Directed Probes for targeting Bacterial Phosphoarginine Phosphatases

  Christian E. Stieger; Yassmine El Harraoui; Luke Brennan; Michael Lisurek; Jan Vincent V. Arafiles; Charlotte Völkel; Kristin Kemnitz-Hassanin; Han Sun; Stephan Sieber; Christian P.R. Hackenberger

  13 Jan. 2026

  
• Intracellular Delivery of Native Proteins by BioReversible Arginine Modification (BioRAM) on Amino Groups

  Jonathan Franke; Jan Vincent V. Arafiles; Christian Leis; Christian P. R. Hackenberger

  Angewandte Chemie International Edition, 18 Aug. 2025

  
• Intrazellulärer Transport nativer Proteine durch Bioreversible Arginin‐Modifikation (BioRAM) primärer Aminogruppen

  Jonathan Franke; Jan Vincent V. Arafiles; Christian Leis; Christian P. R. Hackenberger

  Angewandte Chemie, 18 Aug. 2025

  
• Unsaturated Phosphine Oxides for Modular Antibody Rebridging and Single Reagent Peptide‐Cyclisation‐Bioconjugation

  Christian E. Stieger; Alastair McMillan; Mark A. R. de Geus; Jan Vincent Arafiles; Luise Franz; Christian Hackenberger

  Angewandte Chemie International Edition, 30 Jun. 2025

  
• Orthogonal Site-Specific Dual Bioconjugation of Aryl and Alkyl Thiols

  Mark A. R. de Geus; Christian E. Stieger; Jan Vincent V. Arafiles; Jean-Romain P. J. Lotthé; Peter Schmieder; Kristin Kemnitz-Hassanin; Beate Kindt; Heinrich Leonhardt; Saskia Schmitt; Marcus Gerlach; Dominik Schumacher; Jonas Helma; Marc-André Kasper; Christian P. R. Hackenberger

  Journal of the American Chemical Society, 04 Jun. 2025

  
• Energy-transfer photoproximity labelling in live cells using an organic cofactor

  Leander B. Crocker; Jan Vincent V. Arafiles; Judith M. Müchler; Max Ruwolt; Kristin Kemnitz-Hassanin; Kilian Roßmann; Christian E. Stieger; Fan Liu; Nataliya Archipowa; Roger Jan Kutta; Christian P. R. Hackenberger

  Nature Chemistry, Dec. 2025

  
• Cell-Surface-Retained Peptide Additives for the Cytosolic Delivery of Functional Proteins

  Jan Vincent V. Arafiles; Jonathan Franke; Luise Franz; Jacobo Gómez-González; Kristin Kemnitz-Hassanin; Christian P. R. Hackenberger

  Journal of the American Chemical Society, 31 Oct. 2023

  
• Struktur‐Wirkungsbeziehung von an zyklische Zell‐penetrierende Peptide gebundenen DABCYL‐Derivaten für den Transport von synthetischen Proteinen in lebende Zellen

  Abhishek Saha; Shaswati Mandal; Jan Vincent V. Arafiles; Jacobo Gómez‐González; Christian P. R. Hackenberger; Ashraf Brik

  Angewandte Chemie, 21 Nov. 2022

  
• Structure–Uptake Relationship Study of DABCYL Derivatives Linked to Cyclic Cell‐Penetrating Peptides for Live‐Cell Delivery of Synthetic Proteins

  Abhishek Saha; Shaswati Mandal; Jan Vincent V. Arafiles; Jacobo Gómez‐González; Christian P. R. Hackenberger; Ashraf Brik

  Angewandte Chemie International Edition, 21 Nov. 2022

  
• Stearylated Macropinocytosis-Inducing Peptides Facilitating the Cellular Uptake of Small Extracellular Vesicles

  Yuna Nakagawa; Jan Vincent V. Arafiles; Yoshimasa Kawaguchi; Ikuhiko Nakase; Hisaaki Hirose; Shiroh Futaki

  Bioconjugate Chemistry, 18 May 2022

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• Piezo1 activation using Yoda1 inhibits macropinocytosis in A431 human epidermoid carcinoma cells

  Masashi Kuriyama; Hisaaki Hirose; Toshihiro Masuda; Masachika Shudou; Jan Vincent V. Arafiles; Miki Imanishi; Masashi Maekawa; Yuji Hara; Shiroh Futaki

  Scientific Reports, 15 Apr. 2022

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• Artificial Nanocage Formed via Self-Assembly of β-Annulus Peptide for Delivering Biofunctional Proteins into Cell Interiors

  Kentarou Sakamoto; Hiroto Furukawa; Jan Vincent V. Arafiles; Miki Imanishi; Kazunori Matsuura; Shiroh Futaki

  Bioconjugate Chemistry, 16 Feb. 2022

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• Grafting Hydrophobic Amino Acids Critical for Inhibition of Protein–Protein Interactions on a Cell-Penetrating Peptide Scaffold

  Yuki Nagano; Jan Vincent V. Arafiles; Keiko Kuwata; Yoshimasa Kawaguchi; Miki Imanishi; Hisaaki Hirose; Shiroh Futaki

  Molecular Pharmaceutics, 07 Feb. 2022

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• Modular solid-phase synthesis of electrophilic cysteine-selective ethynyl-phosphonamidate peptides

  Sarah Hansen; Jan Vincent V. Arafiles; Philipp Ochtrop; Christian P. R. Hackenberger

  Chemical Communications, 2022

  
• Liquid Droplet Formation and Facile Cytosolic Translocation of IgG in the Presence of Attenuated Cationic Amphiphilic Lytic Peptides

  Takahiro Iwata; Hisaaki Hirose; Kentarou Sakamoto; Yusuke Hirai; Jan Vincent V. Arafiles; Misao Akishiba; Miki Imanishi; Shiroh Futaki

  Angewandte Chemie, Sep. 2021

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• Cover Picture: Liquid Droplet Formation and Facile Cytosolic Translocation of IgG in the Presence of Attenuated Cationic Amphiphilic Lytic Peptides (Angew. Chem. Int. Ed. 36/2021)

  Takahiro Iwata; Hisaaki Hirose; Kentarou Sakamoto; Yusuke Hirai; Jan Vincent V. Arafiles; Misao Akishiba; Miki Imanishi; Shiroh Futaki

  Angewandte Chemie International Edition, Sep. 2021

  
• Discovery of a Macropinocytosis-Inducing Peptide Potentiated by Medium-Mediated Intramolecular Disulfide Formation

  Arafiles, J.V.V.; Hirose, H.; Hirai, Y.; Kuriyama, M.; Sakyiamah, M.M.; Nomura, W.; Sonomura, K.; Imanishi, M.; Otaka, A.; Tamamura, H.; Futaki, S.

  Angewandte Chemie - International Edition, 17 May 2021

  
• Piezo1 activation using Yoda1 inhibits macropinocytosis and proliferation of cancer cells

  Kuriyama M; Hirose H; Masuda T; Shudou M; Arafiles JVV; Imanishi M; Maekawa M; Hara Y; Futaki S

  May 2021

  
• Use of homoarginine to obtain attenuated cationic membrane lytic peptides.

  Sakamoto K; Akishiba M; Iwata T; Arafiles JVV; Imanishi M; Futaki S

  Bioorganic & medicinal chemistry letters, Mar. 2021

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• Chemical passports to cross biological borders

  Arafiles, J.V.V.; Futaki, S.

  Nature Chemistry, 2021

  
• A facile combinatorial approach to construct a ratiometric fluorescent sensor: application for the real-time sensing of cellular pH changes

  Eiji Nakata; Hisaaki Hirose; Khongorzul Gerelbaatar; Jan Vincent V. Arafiles; Zhengxiao Zhang; Shiroh Futaki; Takashi Morii

  Chemical Science, 2021

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• Enhancing the Activity of Membrane Remodeling Epsin-Peptide by Trimerization

  Jan Vincent V. Arafiles

  Bioorganic & Medicinal Chemistry Letters, Apr. 2020

  
• Peptide-assisted intracellular delivery of biomacromolecules

  Futaki, S.; Arafiles, J.V.V.; Hirose, H.

  Chemistry Letters, 2020

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• Stimulating Macropinocytosis for Intracellular Nucleic Acid and Protein Delivery: A Combined Strategy with Membrane-Lytic Peptides to Facilitate Endosomal Escape

  Arafiles, J.V.V.; Hirose, H.; Akishiba, M.; Tsuji, S.; Imanishi, M.; Futaki, S.

  Bioconjugate Chemistry, 04 Feb. 2020

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Misc.

• Intracellular delivery of extracellular vesicles using acylated macropinocytosis-inducing peptide

  中川優奈; ARAFILES Jan Vincent V.; 広瀬久昭; 二木史朗

  日本薬学会年会要旨集(Web), 2022

  
• Involvement of mechanosensitive channel Piezo1 in EGF-induced macropinocytosis induction

  栗山理志; 広瀬久昭; 益田俊博; ARAFILES Jan Vincent V.; 今西未来; 二木史朗

  日本薬学会年会要旨集(Web), 2021

  
• 機械受容チャネルPiezo1の活性化によるマクロピノサイトーシス阻害とがん細胞増殖抑制

  栗山理志; 広瀬久昭; 益田俊博; ARAFILES Jan Vincent V.; 今西未来; 二木史朗

  創薬懇話会講演要旨集, 2021

  
• 高分子送達ペプチドとのコアセルベート形成による抗体の細胞内送達

  岩田恭宗; 広瀬久昭; 坂本健太郎; 平井勇祐; ARAFILES Jan Vincent V.; 秋柴美沙穂; 今西未来; 二木史朗

  創薬懇話会講演要旨集, 2021

  
• INTRACELLULAR DELIVERY OF ANTIBODIES BY LIQUID-LIQUID PHASE SEPARATION

  IWATA Takahiro; HIROSE Hisaaki; SAKAMOTO Kentarou; HIRAI Yusuke; ARAFILES Jan Vincent V.; AKISHIBA Misao; IMANISHI Miki; FUTAKI Shiroh

  ペプチド討論会講演要旨集, 2021

  
• EXTRACELLULAR THIOL-DISULFIDE EXCHANGE REACTION PRODUCES POTENT MACROPINOCYTOSIS-INDUCING PEPTIDE ANALOGS

  ARAFILES Jan Vincent V.; HIROSE Hisaaki; FUTAKI Shiroh

  ペプチド討論会講演要旨集, 2020

  
• Macropinocytosis-inducing peptide efficiently delivers bioactive macromolecules

  ARAFILES Jan Vincent V.; HIROSE Hisaaki; FUTAKI Shiroh

  日本薬学会年会要旨集(Web), 2020

  
• INTRACELLULAR DELIVERY OF BIOACTIVE MOLECULES VIA MACROPINOCYTOSIS INDUCTION

  ARAFILES Jan Vincent V.; HIROSE Hisaaki; AKISHIBA Misao; FUTAKI Shiroh

  ペプチド討論会講演要旨集, 2019

  
• 新規マクロピノサイトーシス誘導細胞内込達ペプチド

  ARAFILES Jan Vincent; KONO Ken’ichi; FUTAKI Shiro

  日本薬学会年会要旨集(CD-ROM), 2018

  
• A MALDI-TOF mass spectroscopy-based approach to evaluate the cytosolic translocation efficiency of octaarginine-mediated intracellular protein delivery

  ARAFILES Jan Vincent; KAWAGUCHI Yoshimasa; CHERINE Bechara; FRANCOISE Illien; KONO Ken’ichi; FABIENNE Burlina; SANDRINE Sagan; FUTAKI Shiro

  日本薬学会年会要旨集(CD-ROM), 2017

  
• NOVEL MACROPINOCYTOSIS-INDUCING CELL PENETRATING PEPTIDES

  ARAFILES Jan Vincent V.; KAWANO Kenichi; FUTAKI Shiroh

  ペプチド討論会講演要旨集, 2017

  

Presentations

• Live-Cell DarT-Labelling Maps the Dynamic Interactomes of Polyarginine Cell-penetrating Peptides

  7th Annual Peptides and Proteins Symposium Singapore

  
• Cell-surface-retained cell-penetrating peptide additives: An effective intracellular delivery strategy for functional cargoes

  Jan Vincent V. Arafiles

  Cell-Penetrating Peptides: Next generation delivery systems, 11 Nov. 2024

  
• Crossing borders: Peptide tools for protein delivery

  21st SynPepBio Research Training Group Symposium: Cellular delivery, Invited

  
• Cell-surface-retained cell-penetrating peptide additives

  13th International Peptide Symposium/ 15th Australian Peptide Conference

  

Industrial Property Rights

• 特許第7716101号, JP2020029120, 細胞内送達ペプチド

  二木 史朗; アラフィレス,ジャン ヴィンセント; 秋柴 美沙穂

  
• WO2022/180242A3, PCT/EP2022/054869, Cellular uptake of large biomolecules enabled by cell-surface-reactive cell-penetrating peptide additives

  Christian Hackenberger; Anselm Schneider; Martin Lehmann; Jan Vincent Arafiles

  
• WO2021/020462A1, 特願2019-139068, Intracellular Delivery Peptide

  Shiroh Futaki; Jan Vincent Arafiles; Akishiba Misao

  

External funds: Kakenhi

• Synthetic Biomolecular Condensates for Physicochemical Control of Enzyme Catalysis

  Grant-in-Aid for Research Activity Start-up

  0501:Physical chemistry, functional solid state chemistry, organic chemistry, polymers, organic materials, biomolecular chemistry, and related fields

  Kyoto University

  Arafiles JanVincentValenzuela

  From 31 Jul. 2026, To 31 Mar. 2028, Granted

  Phase separation;Condensates;Peptides;Enzymes;Microenvironment

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26K24770/