Basic Information

Affiliated programs (koza)

• Graduate School of Energy Science, エネルギー基礎科学専攻 エネルギー物質科学講座, 助教

Email Address

• Email Address

  arafiles.janvincent.7pkyoto-u.ac.jp

Professional Memberships

• From 2016, To Present

  Japanese Peptide Society
• From 2012, To Present

  Philippine Society for Biochemistry and Molecular Biology
• From 2022, To 2026

  European Peptide Society
• From 2016, To 2021

  Japanese Pharmeceutical Society

Academic Degree

• 27 Sep. 2017

  京都大学修士（薬学）
• 23 Sep. 2020

  京都大学博士（薬学）

Academic Resume (Undergraduate School/Majors)

• University of Santo Tomas, Faculty of Pharmacy, Department of Biochemistry, 卒業

Research History

• From Apr. 2026, To Present

  Kyoto University, Institute of Advanced Energy, Assistant Professor
• From Apr. 2021, To Mar. 2026

  Leibniz Forschungsinstitut für Molekulare Pharmakologie - Berlin, Biomolecule Modification and Delivery, Alexander von Humboldt Postdoctoral Fellow
• From Oct. 2020, To Mar. 2021

  Kyoto University, Institute for Chemical Research, Postdoctoral researcher

Language of Instruction

• Japanese
• English

ID,URL

• ORCID ID

  0000-0002-7733-7563

• J-Global ID

  202601003852051298

researchmap URL

• https://researchmap.jp/jvarafiles

Research

Research Interests

• Quantitative imaging
• Proteomics
• Photoproximity labeling
• Intracellular delivery
• Biomolecular organization
• Chemical Biology
• peptide
• liquid–liquid phase separation (LLPS)

Research Areas

• Nanotechnology/Materials, Nanobioscience
• Life sciences, Bioorganic chemistry
• Life sciences, Applied biochemistry

Papers

• Protein Modifications for Cellular Protein Delivery

  Jan Vincent V. Arafiles; Jonathan Franke; Palina Dubatouka; Luise Franz; Christian P.R. Hackenberger

  Chemical Reviews, 26 Aug. 2026

  
• Polyfluoralkylierte zellpenetrierende Peptid‐Additive verbessern die intrazelluläre Proteinaufnahme durch eine anhaltende und monomere Lipidinteraktion

  Sarah Hansen; Hana Zupan; Ferhat Mutlu; Jan Vincent V. Arafiles; Rubén Cruz; Palina Dubatouka; Kenichi Ataka; Martin Lehmann; Bettina G. Keller; Joachim Heberle; Christian P. R. Hackenberger

  Angewandte Chemie, 01 Jun. 2026

  
• Polyfluoroalkyl‐Tagged Cell‐Penetrating Peptide‐Additives Enhance Intracellular Protein Delivery via Sustained Monomeric Lipid Interaction

  Sarah Hansen; Hana Zupan; Ferhat Mutlu; Jan Vincent V. Arafiles; Rubén Cruz; Palina Dubatouka; Kenichi Ataka; Martin Lehmann; Bettina G. Keller; Joachim Heberle; Christian P. R. Hackenberger

  Angewandte Chemie International Edition, 01 Jun. 2026

  
• Cellular protein delivery through membrane potential driven water pores

  Jonathan Franke; Palina Dubatouka; Amin Yourdkhani; Sahil Soni; Tillmann Utesch; Juliana Serrano; Tolga Soykan; Martin Lehmann; Han Sun; Jan Vincent V. Arafiles; Christian P. R. Hackenberger

  25 Feb. 2026

  
• Modular Vinyl Phosphonamidates for Cysteine-Directed Protein Targeting

  Christian E. Stieger; Charlotte Völkel; Mathias B. Bertelsen; Michael Lisurek; Jan Vincent V. Arafiles; Jonathan Franke; Leander Crocker; Karl Schuppe; Yunjae Lim; Christiane Groneberg; Mathias Christmann; Han Sun; Christian P. R. Hackenberger

  Journal of the American Chemical Society, 25 Feb. 2026

  
• Active Site-Directed Probes for targeting Bacterial Phosphoarginine Phosphatases

  Christian E. Stieger; Yassmine El Harraoui; Luke Brennan; Michael Lisurek; Jan Vincent V. Arafiles; Charlotte Völkel; Kristin Kemnitz-Hassanin; Han Sun; Stephan Sieber; Christian P.R. Hackenberger

  13 Jan. 2026

  
• Intracellular Delivery of Native Proteins by BioReversible Arginine Modification (BioRAM) on Amino Groups

  Jonathan Franke; Jan Vincent V. Arafiles; Christian Leis; Christian P. R. Hackenberger

  Angewandte Chemie International Edition, 18 Aug. 2025

  
• Intrazellulärer Transport nativer Proteine durch Bioreversible Arginin‐Modifikation (BioRAM) primärer Aminogruppen

  Jonathan Franke; Jan Vincent V. Arafiles; Christian Leis; Christian P. R. Hackenberger

  Angewandte Chemie, 18 Aug. 2025

  
• Unsaturated Phosphine Oxides for Modular Antibody Rebridging and Single Reagent Peptide‐Cyclisation‐Bioconjugation

  Christian E. Stieger; Alastair McMillan; Mark A. R. de Geus; Jan Vincent Arafiles; Luise Franz; Christian Hackenberger

  Angewandte Chemie International Edition, 30 Jun. 2025

  
• Orthogonal Site-Specific Dual Bioconjugation of Aryl and Alkyl Thiols

  Mark A. R. de Geus; Christian E. Stieger; Jan Vincent V. Arafiles; Jean-Romain P. J. Lotthé; Peter Schmieder; Kristin Kemnitz-Hassanin; Beate Kindt; Heinrich Leonhardt; Saskia Schmitt; Marcus Gerlach; Dominik Schumacher; Jonas Helma; Marc-André Kasper; Christian P. R. Hackenberger

  Journal of the American Chemical Society, 04 Jun. 2025

  
• Energy transfer photoproximity labelling in live cells using an organic cofactor

  Leander Crocker; Jan Vincent V. Arafiles; Judith M. Müchler; Max Ruwolt; Kristin Kemnitz-Hassanin; Kilian Roßmann; Christian E. Stieger; Fan Liu; Christian P. R. Hackenberger

  Nature Chemistry, 27 Nov. 2024

  
• Cell-Surface-Retained Peptide Additives for the Cytosolic Delivery of Functional Proteins

  Jan Vincent V. Arafiles; Jonathan Franke; Luise Franz; Jacobo Gómez-González; Kristin Kemnitz-Hassanin; Christian P. R. Hackenberger

  Journal of the American Chemical Society, 31 Oct. 2023

  
• Struktur‐Wirkungsbeziehung von an zyklische Zell‐penetrierende Peptide gebundenen DABCYL‐Derivaten für den Transport von synthetischen Proteinen in lebende Zellen

  Abhishek Saha; Shaswati Mandal; Jan Vincent V. Arafiles; Jacobo Gómez‐González; Christian P. R. Hackenberger; Ashraf Brik

  Angewandte Chemie, 21 Nov. 2022

  
• Structure–Uptake Relationship Study of DABCYL Derivatives Linked to Cyclic Cell‐Penetrating Peptides for Live‐Cell Delivery of Synthetic Proteins

  Abhishek Saha; Shaswati Mandal; Jan Vincent V. Arafiles; Jacobo Gómez‐González; Christian P. R. Hackenberger; Ashraf Brik

  Angewandte Chemie International Edition, 21 Nov. 2022

  
• Stearylated Macropinocytosis-Inducing Peptides Facilitating the Cellular Uptake of Small Extracellular Vesicles

  Yuna Nakagawa; Jan Vincent V. Arafiles; Yoshimasa Kawaguchi; Ikuhiko Nakase; Hisaaki Hirose; Shiroh Futaki

  Bioconjugate Chemistry, 18 May 2022

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• Piezo1 activation using Yoda1 inhibits macropinocytosis in A431 human epidermoid carcinoma cells

  Masashi Kuriyama; Hisaaki Hirose; Toshihiro Masuda; Masachika Shudou; Jan Vincent V. Arafiles; Miki Imanishi; Masashi Maekawa; Yuji Hara; Shiroh Futaki

  Scientific Reports, 15 Apr. 2022

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• Artificial Nanocage Formed via Self-Assembly of β-Annulus Peptide for Delivering Biofunctional Proteins into Cell Interiors

  Kentarou Sakamoto; Hiroto Furukawa; Jan Vincent V. Arafiles; Miki Imanishi; Kazunori Matsuura; Shiroh Futaki

  Bioconjugate Chemistry, 16 Feb. 2022

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• Grafting Hydrophobic Amino Acids Critical for Inhibition of Protein–Protein Interactions on a Cell-Penetrating Peptide Scaffold

  Yuki Nagano; Jan Vincent V. Arafiles; Keiko Kuwata; Yoshimasa Kawaguchi; Miki Imanishi; Hisaaki Hirose; Shiroh Futaki

  Molecular Pharmaceutics, 07 Feb. 2022

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• Modular solid-phase synthesis of electrophilic cysteine-selective ethynyl-phosphonamidate peptides

  Sarah Hansen; Jan Vincent V. Arafiles; Philipp Ochtrop; Christian P. R. Hackenberger

  Chemical Communications, 2022

  
• Liquid Droplet Formation and Facile Cytosolic Translocation of IgG in the Presence of Attenuated Cationic Amphiphilic Lytic Peptides

  Takahiro Iwata; Hisaaki Hirose; Kentarou Sakamoto; Yusuke Hirai; Jan Vincent V. Arafiles; Misao Akishiba; Miki Imanishi; Shiroh Futaki

  Angewandte Chemie, Sep. 2021

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• Cover Picture: Liquid Droplet Formation and Facile Cytosolic Translocation of IgG in the Presence of Attenuated Cationic Amphiphilic Lytic Peptides (Angew. Chem. Int. Ed. 36/2021)

  Takahiro Iwata; Hisaaki Hirose; Kentarou Sakamoto; Yusuke Hirai; Jan Vincent V. Arafiles; Misao Akishiba; Miki Imanishi; Shiroh Futaki

  Angewandte Chemie International Edition, Sep. 2021

  
• Discovery of a Macropinocytosis-Inducing Peptide Potentiated by Medium-Mediated Intramolecular Disulfide Formation

  Arafiles, J.V.V.; Hirose, H.; Hirai, Y.; Kuriyama, M.; Sakyiamah, M.M.; Nomura, W.; Sonomura, K.; Imanishi, M.; Otaka, A.; Tamamura, H.; Futaki, S.

  Angewandte Chemie - International Edition, 17 May 2021

  
• Piezo1 activation using Yoda1 inhibits macropinocytosis and proliferation of cancer cells

  Kuriyama M; Hirose H; Masuda T; Shudou M; Arafiles JVV; Imanishi M; Maekawa M; Hara Y; Futaki S

  May 2021

  
• Use of homoarginine to obtain attenuated cationic membrane lytic peptides.

  Sakamoto K; Akishiba M; Iwata T; Arafiles JVV; Imanishi M; Futaki S

  Bioorganic & medicinal chemistry letters, Mar. 2021

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• Chemical passports to cross biological borders

  Arafiles, J.V.V.; Futaki, S.

  Nature Chemistry, 2021

  
• A facile combinatorial approach to construct a ratiometric fluorescent sensor: application for the real-time sensing of cellular pH changes

  Eiji Nakata; Hisaaki Hirose; Khongorzul Gerelbaatar; Jan Vincent V. Arafiles; Zhengxiao Zhang; Shiroh Futaki; Takashi Morii

  Chemical Science, 2021

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• Enhancing the Activity of Membrane Remodeling Epsin-Peptide by Trimerization

  Jan Vincent V. Arafiles

  Bioorganic & Medicinal Chemistry Letters, Apr. 2020

  
• Peptide-assisted intracellular delivery of biomacromolecules

  Futaki, S.; Arafiles, J.V.V.; Hirose, H.

  Chemistry Letters, 2020

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• Stimulating Macropinocytosis for Intracellular Nucleic Acid and Protein Delivery: A Combined Strategy with Membrane-Lytic Peptides to Facilitate Endosomal Escape

  Arafiles, J.V.V.; Hirose, H.; Akishiba, M.; Tsuji, S.; Imanishi, M.; Futaki, S.

  Bioconjugate Chemistry, 04 Feb. 2020

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Misc.

• Intracellular delivery of extracellular vesicles using acylated macropinocytosis-inducing peptide

  中川優奈; ARAFILES Jan Vincent V.; 広瀬久昭; 二木史朗

  日本薬学会年会要旨集(Web), 2022

  
• Involvement of mechanosensitive channel Piezo1 in EGF-induced macropinocytosis induction

  栗山理志; 広瀬久昭; 益田俊博; ARAFILES Jan Vincent V.; 今西未来; 二木史朗

  日本薬学会年会要旨集(Web), 2021

  
• 機械受容チャネルPiezo1の活性化によるマクロピノサイトーシス阻害とがん細胞増殖抑制

  栗山理志; 広瀬久昭; 益田俊博; ARAFILES Jan Vincent V.; 今西未来; 二木史朗

  創薬懇話会講演要旨集, 2021

  
• 高分子送達ペプチドとのコアセルベート形成による抗体の細胞内送達

  岩田恭宗; 広瀬久昭; 坂本健太郎; 平井勇祐; ARAFILES Jan Vincent V.; 秋柴美沙穂; 今西未来; 二木史朗

  創薬懇話会講演要旨集, 2021

  
• INTRACELLULAR DELIVERY OF ANTIBODIES BY LIQUID-LIQUID PHASE SEPARATION

  IWATA Takahiro; HIROSE Hisaaki; SAKAMOTO Kentarou; HIRAI Yusuke; ARAFILES Jan Vincent V.; AKISHIBA Misao; IMANISHI Miki; FUTAKI Shiroh

  ペプチド討論会講演要旨集, 2021

  
• EXTRACELLULAR THIOL-DISULFIDE EXCHANGE REACTION PRODUCES POTENT MACROPINOCYTOSIS-INDUCING PEPTIDE ANALOGS

  ARAFILES Jan Vincent V.; HIROSE Hisaaki; FUTAKI Shiroh

  ペプチド討論会講演要旨集, 2020

  
• Macropinocytosis-inducing peptide efficiently delivers bioactive macromolecules

  ARAFILES Jan Vincent V.; HIROSE Hisaaki; FUTAKI Shiroh

  日本薬学会年会要旨集(Web), 2020

  
• INTRACELLULAR DELIVERY OF BIOACTIVE MOLECULES VIA MACROPINOCYTOSIS INDUCTION

  ARAFILES Jan Vincent V.; HIROSE Hisaaki; AKISHIBA Misao; FUTAKI Shiroh

  ペプチド討論会講演要旨集, 2019

  
• 新規マクロピノサイトーシス誘導細胞内込達ペプチド

  ARAFILES Jan Vincent; KONO Ken’ichi; FUTAKI Shiro

  日本薬学会年会要旨集(CD-ROM), 2018

  
• A MALDI-TOF mass spectroscopy-based approach to evaluate the cytosolic translocation efficiency of octaarginine-mediated intracellular protein delivery

  ARAFILES Jan Vincent; KAWAGUCHI Yoshimasa; CHERINE Bechara; FRANCOISE Illien; KONO Ken’ichi; FABIENNE Burlina; SANDRINE Sagan; FUTAKI Shiro

  日本薬学会年会要旨集(CD-ROM), 2017

  
• NOVEL MACROPINOCYTOSIS-INDUCING CELL PENETRATING PEPTIDES

  ARAFILES Jan Vincent V.; KAWANO Kenichi; FUTAKI Shiroh

  ペプチド討論会講演要旨集, 2017

  

Industrial Property Rights

• 特許第7716101号, JP2020029120, 細胞内送達ペプチド

  二木 史朗; アラフィレス,ジャン ヴィンセント; 秋柴 美沙穂

  
• WO2022/180242A3, PCT/EP2022/054869, Cellular uptake of large biomolecules enabled by cell-surface-reactive cell-penetrating peptide additives

  Christian Hackenberger; Anselm Schneider; Martin Lehmann; Jan Vincent Arafiles

  
• WO2021/020462A1, 特願2019-139068, Intracellular Delivery Peptide

  Shiroh Futaki; Jan Vincent Arafiles; Akishiba Misao

  

External funds: Kakenhi

• Synthetic Biomolecular Condensates for Physicochemical Control of Enzyme Catalysis

  Grant-in-Aid for Research Activity Start-up

  0501:Physical chemistry, functional solid state chemistry, organic chemistry, polymers, organic materials, biomolecular chemistry, and related fields

  Kyoto University

  Arafiles JanVincentValenzuela

  From 31 Jul. 2026, To 31 Mar. 2028, Adopted

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26K24770/