Basic Information

Affiliated programs (koza)

• Graduate School of Engineering, 物質エネルギー化学専攻 物質変換科学講座, 助教

Academic Degree

• (Nagoya University)
• (Nagoya University)

Research History

• From Apr. 2021, To Present

  Kyoto University, Institute for Chemical Research International Research Center for Elements Science, Assistant Professor
• From Jun. 2019, To Feb. 2021

  University of California, Irvine, Department of Molecular Biology and Biochemistry, Assistant Project Scientist
• From Jul. 2014, To Jun. 2019

  University of California, Irvine, Department of Molecular Biology and Biochemistry, Postdoctoral Researcher
• From Apr. 2014, To Jun. 2014

  Nagoya University, Research Center for Materials Science, Postdoctoral Researcher

Language of Instruction

• Japanese
• English

ID,URL

• ORCID ID

  0000-0003-1500-1332

• J-Global ID

  202101019478961142

• External link

  https://www.om.kuicr.kyoto-u.ac.jp/the-team

researchmap URL

• https://researchmap.jp/tanifuji-kazuki

Research

Research Topics, Overview of the research

• Research Topics

  遷移金属クラスターを用いた高難度分子変換反応の開発

Papers

• Synthesis of Cubic [Mo₃S₄M] (M = Rh, Ir) Clusters for the Borylation of C–H Bonds in Aromatic Compounds

  Hitoshi Izu; Sayaka Shimoyama; Kazuki Tanifuji; Yasuhiro Ohki

  Organometallics, 11 Sep. 2024, Peer-reviewed, Invited

  
• Silylation of N2 catalyzed by cubic [Mo3S4Ni] clusters bearing Mo-bound cyclopentadienyl ligands

  Yuto Matsuoka; Yuta Sakai; Hitoshi Izu; Sayaka Shimoyama; Mayu Fujisawa; Mizuki Tada; Nishshanka M. Lakshan; W.M.C. Sameera; Kazuki Tanifuji; Yasuhiro Ohki

  Coordination Chemistry Research, Aug. 2024, Peer-reviewed

  
• Reversible transformation between cuboidal Fe4S4 and dinuclear Fe2S2 cores

  Kazuki Tanifuji

  JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY

  
• Biosynthesis of the M-Cluster of Mo-Nitrogenase

  Kazuki Tanifuji

  MOLYBDENUM AND TUNGSTEN ENZYMES: BIOCHEMISTRY

  
• Synthesis, Characterizations, and Catalytic Activity of a Cubic [Mo3S4Pd] Cluster Bearing Bulky Cyclopentadienyl Ligands

  Hitoshi Izu; Devashish Girish Bhave; Yuto Matsuoka; W. M. C. Sameera; Kazuki Tanifuji; Yasuhiro Ohki

  European Journal of Inorganic Chemistry, 31 Jul. 2023, Peer-reviewed, Invited

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• Activation of unsaturated small molecules by bio-relevant multinuclear metal-sulfur clusters

  Kazuki Tanifuji; Shun Ohta; Yasuhiro Ohki; Hidetake Seino

  Coordination Chemistry Reviews, 15 Jan. 2023, Peer-reviewed, Invited, Lead author

  
• CO Binding onto Heterometals of [Mo₃S₄M] (M = Fe, Co, Ni) Cubes

  Kazuki Tanifuji; Yuta Sakai; Yuto Matsuoka; Mizuki Tada; W. M. C. Sameera; Yasuhiro Ohki

  Bulletin of the Chemical Society of Japan, 15 Aug. 2022, Peer-reviewed, Lead author

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• Incorporation of an Asymmetric Mo-Fe-S Cluster as an Artificial Cofactor into Nitrogenase.

  Kazuki Tanifuji; Andrew Jasniewski; Chi Chung Lee; Joseph Solomon; Takayuki Nagasawa; Yasuhiro Ohki; Kazuyuki Tatsumi; Britt Hedman; Keith Hodgson; Yilin Hu; Markus Walter Ribbe

  Chembiochem : a European journal of chemical biology, 04 Aug. 2022, Peer-reviewed, Lead author

  
• Nitrogen reduction by the Fe sites of synthetic [Mo3S4Fe] cubes

  Yasuhiro Ohki; Kenichiro Munakata; Yuto Matsuoka; Ryota Hara; Mami Kachi; Keisuke Uchida; Mizuki Tada; Roger E. Cramer; W. M. C. Sameera; Tsutomu Takayama; Yoichi Sakai; Shogo Kuriyama; Yoshiaki Nishibayashi; Kazuki Tanifuji

  Nature, 07 Jul. 2022, Peer-reviewed

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• Evidence of substrate binding and product release via belt-sulfur mobilization of the nitrogenase cofactor

  Chi Chung Lee; Wonchull Kang; Andrew J. Jasniewski; Martin T. Stiebritz; Kazuki Tanifuji; Markus W. Ribbe; Yilin Hu

  Nature Catalysis, May 2022, Peer-reviewed

  
• Characterization of a Nitrogenase Iron Protein Substituted with a Synthetic [Fe4Se4] Cluster.

  Joseph Solomon; Kazuki Tanifuji; Chi Chung Lee; Andrew Jasniewski; Britt Hedman; Keith Hodgson; Yilin Hu; Markus Ribbe

  Angewandte Chemie (International ed. in English), 25 Feb. 2022, Peer-reviewed, Lead author

  
• Tracing the incorporation of the "ninth sulfur" into the nitrogenase cofactor precursor with selenite and tellurite.

  Kazuki Tanifuji; Andrew J Jasniewski; David Villarreal; Martin T Stiebritz; Chi Chung Lee; Jarett Wilcoxen; Yasuhiro Okhi; Ruchira Chatterjee; Isabel Bogacz; Junko Yano; Jan Kern; Britt Hedman; Keith O Hodgson; R David Britt; Yilin Hu; Markus W Ribbe

  Nature chemistry, 11 Oct. 2021, Peer-reviewed, Lead author

  
• An EPR and VTVH MCD spectroscopic investigation of the nitrogenase assembly protein NifB

  Kresimir Rupnik; Lee Rettberg; Kazuki Tanifuji; Johannes G. Rebelein; Markus W. Ribbe; Yilin Hu; Brian J. Hales

  JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, Jun. 2021, Peer-reviewed

  
• Probing the All-Ferrous States of Methanogen Nitrogenase Iron Proteins

  Joseph B. Solomon; Mahtab F. Rasekh; Caleb J. Hiller; Chi Chung Lee; Kazuki Tanifuji; Markus W. Ribbe; Yilin Hu

  JACS AU, Feb. 2021, Peer-reviewed

  
• X-Ray Crystallographic Analysis of NifB with a Full Complement of Clusters: Structural Insights into the Radical SAM-Dependent Carbide Insertion During Nitrogenase Cofactor Assembly.

  Wonchull Kang; Lee A Rettberg; Martin T Stiebritz; Andrew J Jasniewski; Kazuki Tanifuji; Chi Chung Lee; Markus W Ribbe; Yilin Hu

  Angewandte Chemie (International ed. in English), 01 Feb. 2021, Peer-reviewed

  
• Characterization of a Mo-Nitrogenase Variant Containing a Citrate-Substituted Cofactor.

  Jasper Liedtke; Chi Chung Lee; Kazuki Tanifuji; Andrew J Jasniewski; Markus W Ribbe; Yilin Hu

  Chembiochem : a European journal of chemical biology, 05 Jan. 2021, Peer-reviewed

  
• Metal-Sulfur Compounds in N2 Reduction and Nitrogenase-Related Chemistry.

  Kazuki Tanifuji; Yasuhiro Ohki

  Chemical reviews, 24 Jun. 2020, Peer-reviewed, Invited

  
• Electrochemical Characterization of Isolated Nitrogenase Cofactors from Azotobacter vinelandii.

  Brian R Lydon; Chi Chung Lee; Kazuki Tanifuji; Nathaniel S Sickerman; Megan P Newcomb; Yilin Hu; Markus W Ribbe; Jenny Y Yang

  Chembiochem : a European journal of chemical biology, 15 Jun. 2020, Peer-reviewed

  
• A V-Nitrogenase Variant Containing a Citrate-Substituted Cofactor.

  Megan P Newcomb; Chi Chung Lee; Kazuki Tanifuji; Andrew J Jasniewski; Jasper Liedtke; Markus W Ribbe; Yilin Hu

  Chembiochem : a European journal of chemical biology, 15 Jun. 2020, Peer-reviewed

  
• Electron Paramagnetic Resonance and Magnetic Circular Dichroism Spectra of the Nitrogenase M Cluster Precursor Suggest Sulfur Migration upon Oxidation: A Proposal for Substrate and Inhibitor Binding.

  Kresimir Rupnik; Kazuki Tanifuji; Lee Rettberg; Markus W Ribbe; Yilin Hu; Brian J Hales

  Chembiochem : a European journal of chemical biology, 15 Jun. 2020, Peer-reviewed

  
• Identity and function of an essential nitrogen ligand of the nitrogenase cofactor biosynthesis protein NifB.

  Lee A Rettberg; Jarett Wilcoxen; Andrew J Jasniewski; Chi Chung Lee; Kazuki Tanifuji; Yilin Hu; R David Britt; Markus W Ribbe

  Nature communications, 09 Apr. 2020, Peer-reviewed

  
• Spectroscopic Characterization of an Eight-Iron Nitrogenase Cofactor Precursor that Lacks the "9th Sulfur".

  Andrew J Jasniewski; Jarett Wilcoxen; Kazuki Tanifuji; Britt Hedman; Keith O Hodgson; R David Britt; Yilin Hu; Markus W Ribbe

  Angewandte Chemie (International ed. in English), 07 Oct. 2019, Peer-reviewed

  
• Chemical Synthesis of an Asymmetric Mimic of the Nitrogenase Active Site.

  Kazuki Tanifuji; Yasuhiro Ohki

  Methods in molecular biology (Clifton, N.J.), 2019, Invited

  
• Probing the coordination and function of Fe4S4 modules in nitrogenase assembly protein NifB.

  Lee A Rettberg; Jarett Wilcoxen; Chi Chung Lee; Martin T Stiebritz; Kazuki Tanifuji; R David Britt; Yilin Hu

  Nature communications, 19 Jul. 2018, Peer-reviewed

  
• Ambient conversion of CO2 to hydrocarbons by biogenic and synthetic [Fe4S4] clusters

  Martin T. Stiebritz; Caleb J. Hiller; Nathaniel S. Sickerman; Chi Chung Lee; Kazuki Tanifuji; Yasuhiro Ohki; Yilin Hu

  Nature Catalysis, Jun. 2018, Peer-reviewed

  
• Tracing the 'ninth sulfur' of the nitrogenase cofactor via a semi-synthetic approach.

  Kazuki Tanifuji; Chi Chung Lee; Nathaniel S Sickerman; Kazuyuki Tatsumi; Yasuhiro Ohki; Yilin Hu; Markus W Ribbe

  Nature chemistry, May 2018, Peer-reviewed

  
• A Comparative Analysis of the CO-Reducing Activities of MoFe Proteins Containing Mo- and V-Nitrogenase Cofactors.

  Chi Chung Lee; Kazuki Tanifuji; Megan Newcomb; Jasper Liedtke; Yilin Hu; Markus W Ribbe

  Chembiochem : a European journal of chemical biology, 04 Apr. 2018, Peer-reviewed

  
• Recent Advances in the Chemical Synthesis of Nitrogenase Model Clusters

  Kazuki Tanifuji; Yasuhiro Ohki

  Structure and Bonding, 2018, Invited

  
• Radical S-Adenosyl-l-Methionine (SAM) Enzyme Involved in the Maturation of the Nitrogenase Cluster.

  Lee Rettberg; Kazuki Tanifuji; Andrew Jasniewski; Markus Walter Ribbe; Yilin Hu

  Methods in enzymology, 2018, Invited

  
• Synthetic Analogues of Nitrogenase Metallocofactors: Challenges and Developments.

  Nathaniel S Sickerman; Kazuki Tanifuji; Yilin Hu; Markus W Ribbe

  Chemistry (Weinheim an der Bergstrasse, Germany), 12 Sep. 2017, Peer-reviewed, Invited

  
• Reduction of C1 Substrates to Hydrocarbons by the Homometallic Precursor and Synthetic Mimic of the Nitrogenase Cofactor.

  Nathaniel S Sickerman; Kazuki Tanifuji; Chi Chung Lee; Yasuhiro Ohki; Kazuyuki Tatsumi; Markus W Ribbe; Yilin Hu

  Journal of the American Chemical Society, 18 Jan. 2017, Peer-reviewed

  
• Structure and Reactivity of an Asymmetric Synthetic Mimic of Nitrogenase Cofactor.

  Kazuki Tanifuji; Nathaniel Sickerman; Chi Chung Lee; Takayuki Nagasawa; Kosuke Miyazaki; Yasuhiro Ohki; Kazuyuki Tatsumi; Yilin Hu; Markus W Ribbe

  Angewandte Chemie (International ed. in English), 12 Dec. 2016, Peer-reviewed

  
• Interconversion between [Fe4S4] and [Fe2S2] Clusters Bearing Amide Ligands.

  Kazuki Tanifuji; Shunichi Tajima; Yasuhiro Ohki; Kazuyuki Tatsumi

  Inorganic chemistry, 02 May 2016, Peer-reviewed

  
• Combining a Nitrogenase Scaffold and a Synthetic Compound into an Artificial Enzyme.

  Kazuki Tanifuji; Chi Chung Lee; Yasuhiro Ohki; Kazuyuki Tatsumi; Yilin Hu; Markus W Ribbe

  Angewandte Chemie (International ed. in English), 16 Nov. 2015, Peer-reviewed

  
• A convenient route to synthetic analogues of the oxidized form of high-potential iron-sulfur proteins.

  Kazuki Tanifuji; Norihiro Yamada; Tomoyuki Tajima; Takahiro Sasamori; Norihiro Tokitoh; Tsukasa Matsuo; Kohei Tamao; Yasuhiro Ohki; Kazuyuki Tatsumi

  Inorganic chemistry, 21 Apr. 2014, Peer-reviewed

  
• Formation of a nitrogenase P-cluster [Fe8S7] core via reductive fusion of two all-ferric [Fe4S4] clusters.

  Yasuhiro Ohki; Kazuki Tanifuji; Norihiro Yamada; Roger E Cramer; Kazuyuki Tatsumi

  Chemistry, an Asian journal, Oct. 2012, Peer-reviewed

  
• Non-centrosymmetric coordination polymer with a highly hindered octahedral copper center bridged by mandelate.

  Makoto Moriya; Shingo Tominaga; Takayoshi Hashimoto; Kazuki Tanifuji; Tsuyoshi Matsumoto; Yasuhiro Ohki; Kazuyuki Tatsumi; Junichi Kaneshiro; Yoshiaki Uesu; Wataru Sakamoto; Toshinobu Yogo

  Inorganic chemistry, 16 Apr. 2012, Peer-reviewed

  
• Synthetic analogues of [Fe4S4(Cys)3(His)] in hydrogenases and [Fe4S4(Cys)4] in HiPIP derived from all-ferric [Fe4S4{N(SiMe3)2}4].

  Yasuhiro Ohki; Kazuki Tanifuji; Norihiro Yamada; Motosuke Imada; Tomoyuki Tajima; Kazuyuki Tatsumi

  Proceedings of the National Academy of Sciences of the United States of America, 02 Aug. 2011, Peer-reviewed

  

Misc.

• 窒素を還元するクラスターはどう作られ, どう働くか？

  谷藤一樹

  化学と工業, Jul. 2024, Invited, Lead author, Last author, Corresponding author

  
• 自然界に学金属-硫黄原子配列と酵素模倣機能

  谷藤一樹; 大木靖弘

  化学と工業, Jun. 2024, Invited, Lead author

  
• Studies on Biosynthesis and Catalysis of Metal-Sulfur Clusters

  Kazuki Tanifuji

  Bulletin of Japan Society of Coordination Chemistry, 31 May 2023, Lead author, Last author, Corresponding author

  
• ニトロゲナーゼの活性中心を模した合成金属錯体による窒素分子の還元

  谷藤 一樹; 大木 靖弘

  バイオサイエンスとインダストリー, Jan. 2023, Lead author

  
• 酵素を模倣した金属-硫⻩化合物による窒素の還元

  大木 靖弘; 谷藤 一樹

  クリーンエネルギー, Dec. 2022

  
• 金属-硫黄クラスターを用いた窒素還元，ついに成功！

  谷藤一樹; 大木靖弘

  化学, Nov. 2022, Lead author

  
• 有機金属化学に関わる金属-硫黄クラスターによる小分子の活性化および変換反応—Metal-Sulfur Clusters with Relevance to Organometallic Chemistry for Small Molecule Activation and Transformation

  谷藤 一樹; 大木 靖弘; 清野 秀岳

  有機合成化学協会誌 = Journal of synthetic organic chemistry, Japan, Sep. 2022

  

Awards

• 17 Apr. 2024

  文部科学省, 令和６年度科学技術分野の文部科学大臣表彰若手科学者賞
• 28 Sep. 2022

  錯体化学会, 研究奨励賞(錯体化学会)

External funds: Kakenhi

• Structure-function relationships of a Fe/Mo-S-C cluster unique to N2-reducing enzymes

  Grant-in-Aid for Research Activity Start-up

  0502:Inorganic/coordination chemistry, analytical chemistry, inorganic materials chemistry, energy-related chemistry, and related fields

  Kyoto University

  Kazuki Tanifuji

  From 30 Aug. 2021, To 31 Mar. 2023, Project Closed

  窒素固定;鉄;モリブデン;硫黄;炭素;クラスター;窒素還元酵素（ニトロゲナーゼ）

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K20557/
• CO2/CO reduction into short-chain hydrocarbons promoted by cuboidal metal-sulfur clusters

  Grant-in-Aid for Scientific Research (C)

  Basic Section 34010:Inorganic/coordination chemistry-related

  Kyoto University

  谷藤 一樹

  From 01 Apr. 2022, To 31 Mar. 2025, Granted

  クラスター;卑金属;モリブデン;硫黄;二酸化炭素;還元;CO2還元;金属-硫黄クラスター;第一遷移金属

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K05143/
• 触媒応用を志向したキュバン型金属-カルコゲニドクラスターの構造多様化

  Grant-in-Aid for Transformative Research Areas (A)

  Transformative Research Areas, Section (II)

  Kyoto University

  谷藤 一樹

  From 01 Apr. 2025, To 31 Mar. 2027, Adopted

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-25H02027/

Education

Teaching subject(s)

• From 01 Apr. 2023, To 31 Mar. 2024

  Energy and Hydrocarbon Chemistry, Adv.1

  D220, Spring, Graduate School of Engineering, 1.5
• From 01 Apr. 2022, To 31 Mar. 2023

  Fundamental Chemical Experiments

  N308, Spring, Institute for Liberal Arts and Sciences, 2

Part-time lecturer

• From 01 Oct. 2024, To 31 Mar. 2025

  先端化学実験Ⅱ（先端化学）

  後期96時間 , 京都大学, 工学部
• From 01 Oct. 2023, To 31 Mar. 2024

  先端化学実験Ⅱ（先端化学）

  後期96時間, 京都大学, 工学部
• From 01 Oct. 2022, To 31 Mar. 2023

  先端化学実験Ⅱ（先端化学）

  後期96時間, 工学部

Administration

Faculty management (title, position)

• From 01 Apr. 2022, To 31 Mar. 2023

  化学研究所講演委員会委員