Basic Information

Academic Degree

• 23 Mar. 2009

  京都大学修士（医科学）
• 24 Mar. 2014

  京都大学博士（医科学）

Academic Resume (Graduate Schools)

• 京都大学, 大学院医学研究科博士後期課程医科学専攻, 修了
• 京都大学, 大学院医学研究科博士後期課程医科学専攻, 研究指導認定退学
• 京都大学, 大学院医学研究科修士課程医科学専攻, 修了

Academic Resume (Undergraduate School/Majors)

• 京都大学, 農学部資源生物科学科, 卒業

Research History

• From Apr. 2023, To Present

  Kyoto University, Hakubi Center, Assistant professor, Hakubi Researcher
• From Dec. 2022, To Mar. 2023

  Kyoto University, Institute for the Advanced Study of Human Biology (ASHBi), Program-specific Researcher
• From Jun. 2014, To Nov. 2022

  Ludwig Maximilian University of Munich, Department Biology, Anthropology and Human Genomics, Post-Doc Researcher
• From Apr. 2015, To Mar. 2017

  Japan Society for the Promotion of Science, Overseas Fellow
• From Apr. 2012, To May 2014

  Kyoto University, Center for iPS Cell Research and Application (CiRA), Program-specific Researcher
• From Apr. 2009, To Mar. 2012

  Japan Society for the Promotion of Science, Research Fellow DC1

ID,URL

• ORCID ID

  0000-0001-5478-6297

• J-Global ID

  202301017709930050

• Researcher number

  40983606

researchmap URL

• https://researchmap.jp/ohnukimari

Research

Research Interests

• Regulatory element
• Evolution
• Primate
• Transposable element
• iPS cell
• Reprogramming

Papers

• Evidence for compensatory evolution within pleiotropic regulatory elements

  Zane Kliesmete; Peter Orchard; Victor Yan Kin Lee; Johanna Geuder; Simon M. Krauß; Mari Ohnuki; Jessica Jocher; Beate Vieth; Wolfgang Enard; Ines Hellmann

  Genome Research, Oct. 2024

  
• Evidence for compensatory evolution within pleiotropic regulatory elements

  Zane Kliesmete; Peter Orchard; Victor Yan Kin Lee; Johanna Geuder; Simon M. Krauß; Mari Ohnuki; Jessica Jocher; Beate Vieth; Wolfgang Enard; Ines Hellmann

  11 Jan. 2024

  
• Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi.

  Fiona C Edenhofer; Anita Térmeg; Mari Ohnuki; Jessica Jocher; Zane Kliesmete; Eva Briem; Ines Hellmann; Wolfgang Enard

  iScience, 21 Jun. 2024, Peer-reviewed

  
• Generation of chimpanzee induced pluripotent stem cell lines for cross-species comparisons.

  Masanori Imamura; Risako Nakai; Mari Ohnuki; Yusuke Hamazaki; Hideyuki Tanabe; Momoka Sato; Yu Harishima; Musashi Horikawa; Mao Watanabe; Hiroki Oota; Masato Nakagawa; Shunsuke Suzuki; Wolfgang Enard

  In vitro cellular & developmental biology. Animal, 22 Feb. 2024, Peer-reviewed

  
• Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine.

  Jessica Radmer; Johanna Geuder; Fiona C Edenhofer; Wolfgang Enard; Mari Ohnuki

  Journal of visualized experiments : JoVE, 28 Jul. 2023, Peer-reviewed

  
• Regulatory and coding sequences of TRNP1 co-evolve with brain size and cortical folding in mammals.

  Zane Kliesmete; Lucas Esteban Wange; Beate Vieth; Miriam Esgleas; Jessica Radmer; Matthias Hülsmann; Johanna Geuder; Daniel Richter; Mari Ohnuki; Magdelena Götz; Ines Hellmann; Wolfgang Enard

  eLife, 22 Mar. 2023, Peer-reviewed

  
• The pluripotent stem cell-specific transcript ESRG is dispensable for human pluripotency.

  Kazutoshi Takahashi; Michiko Nakamura; Chikako Okubo; Zane Kliesmete; Mari Ohnuki; Megumi Narita; Akira Watanabe; Mai Ueda; Yasuhiro Takashima; Ines Hellmann; Shinya Yamanaka

  PLoS genetics, May 2021, Peer-reviewed

  Access this paper :

  
• A non-invasive method to generate induced pluripotent stem cells from primate urine.

  Johanna Geuder; Lucas E Wange; Aleksandar Janjic; Jessica Radmer; Philipp Janssen; Johannes W Bagnoli; Stefan Müller; Artur Kaul; Mari Ohnuki; Wolfgang Enard

  Scientific reports, 10 Feb. 2021, Peer-reviewed

  
• Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata).

  Risako Nakai; Mari Ohnuki; Kota Kuroki; Haruka Ito; Hirohisa Hirai; Ryunosuke Kitajima; Toko Fujimoto; Masato Nakagawa; Wolfgang Enard; Masanori Imamura

  Scientific reports, 15 Aug. 2018, Peer-reviewed

  Access this paper :

  
• Epigenetic Variation between Human Induced Pluripotent Stem Cell Lines Is an Indicator of Differentiation Capacity.

  Masatoshi Nishizawa; Kazuhisa Chonabayashi; Masaki Nomura; Azusa Tanaka; Masahiro Nakamura; Azusa Inagaki; Misato Nishikawa; Ikue Takei; Akiko Oishi; Koji Tanabe; Mari Ohnuki; Hidaka Yokota; Michiyo Koyanagi-Aoi; Keisuke Okita; Akira Watanabe; Akifumi Takaori-Kondo; Shinya Yamanaka; Yoshinori Yoshida

  Cell stem cell, 01 Sep. 2016

  
• Present and future challenges of induced pluripotent stem cells.

  Mari Ohnuki; Kazutoshi Takahashi

  Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 19 Oct. 2015

  
• Dynamic regulation of human endogenous retroviruses mediates factor-induced reprogramming and differentiation potential.

  Mari Ohnuki; Koji Tanabe; Kenta Sutou; Ito Teramoto; Yuka Sawamura; Megumi Narita; Michiko Nakamura; Yumie Tokunaga; Masahiro Nakamura; Akira Watanabe; Shinya Yamanaka; Kazutoshi Takahashi

  Proceedings of the National Academy of Sciences of the United States of America, 26 Aug. 2014

  
• Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm.

  Kazutoshi Takahashi; Koji Tanabe; Mari Ohnuki; Megumi Narita; Aki Sasaki; Masamichi Yamamoto; Michiko Nakamura; Kenta Sutou; Kenji Osafune; Shinya Yamanaka

  Nature communications, 24 Apr. 2014

  
• Differentiation-defective phenotypes revealed by large-scale analyses of human pluripotent stem cells.

  Michiyo Koyanagi-Aoi; Mari Ohnuki; Kazutoshi Takahashi; Keisuke Okita; Hisashi Noma; Yuka Sawamura; Ito Teramoto; Megumi Narita; Yoshiko Sato; Tomoko Ichisaka; Naoki Amano; Akira Watanabe; Asuka Morizane; Yasuhiro Yamada; Tosiya Sato; Jun Takahashi; Shinya Yamanaka

  Proceedings of the National Academy of Sciences of the United States of America, 17 Dec. 2013

  
• Variation in the safety of induced pluripotent stem cell lines.

  Kyoko Miura; Yohei Okada; Takashi Aoi; Aki Okada; Kazutoshi Takahashi; Keisuke Okita; Masato Nakagawa; Michiyo Koyanagi; Koji Tanabe; Mari Ohnuki; Daisuke Ogawa; Eiji Ikeda; Hideyuki Okano; Shinya Yamanaka

  Nature biotechnology, Aug. 2009

  
• Generation and characterization of human induced pluripotent stem cells.

  Mari Ohnuki; Kazutoshi Takahashi; Shinya Yamanaka

  Current protocols in stem cell biology, Jun. 2009

  
• Induction of pluripotent stem cells from adult human fibroblasts by defined factors.

  Kazutoshi Takahashi; Koji Tanabe; Mari Ohnuki; Megumi Narita; Tomoko Ichisaka; Kiichiro Tomoda; Shinya Yamanaka

  Cell, 30 Nov. 2007

  
• Reprogramming events of mammalian somatic cells induced by Xenopus laevis egg extracts.

  Kei Miyamoto; Tadashi Furusawa; Mari Ohnuki; Sandeep Goel; Tomoyuki Tokunaga; Naojiro Minami; Masayasu Yamada; Keita Ohsumi; Hiroshi Imai

  Molecular reproduction and development, Oct. 2007

  
• Nuclear reprogramming of porcine cells and their use as donor cell for nuclear transfer after treatment in Xenopus egg extracts

  K. Miyamoto; M. Ohnuki; N. Minami; M. Yamada; H. Imai

  REPRODUCTION FERTILITY AND DEVELOPMENT, 2007, Peer-reviewed

  
• A defect in protein farnesylation suppresses a loss of Schizosaccharomyces pombe tsc2+, a homolog of the human gene predisposing to tuberous sclerosis complex.

  Yukiko Nakase; Keiko Fukuda; Yuji Chikashige; Chihiro Tsutsumi; Daisuke Morita; Shinpei Kawamoto; Mari Ohnuki; Yasushi Hiraoka; Tomohiro Matsumoto

  Genetics, Jun. 2006

  

Misc.

• A defect in protein farnesylation suppresses a loss of Schizosaccharomyces pombe tsc2(+), a homolog of the human gene predisposing to tuberous sclerosis complex (vol 173, pg 569, 2006)

  Y. Nakase; K. Fukuda; Y. Chikashige; C. Tsutsumi; D. Morita; S. Kawamoto; M. Ohnuki; Y. Hiraoka; T. Matsumoto

  GENETICS, Aug. 2006

  

Industrial Property Rights

• 特許第6143265号, 特願2014-503890, 人工多能性幹細胞の選別方法

  山中 伸弥; 高橋 和利; 小柳 三千代; 大貫 茉里

  
• 特表2016-520288, 特願2015-560441, 効率的な人工多能性幹細胞の樹立方法

  山中 伸弥; 高橋 和利; 田邊 剛士; 大貫 茉里

  
• 特許第5936134号, 特願2012-556312, ヒト人工多能性幹細胞の選択方法

  山中 伸弥; 高橋 和利; 大貫 茉里

  
• 特許第5682043号, 特願2012-512108, 安全な多能性幹細胞の選択方法

  山中 伸弥; 大貫 茉里

  
• 特表2014-523735, 特願2014-503890, 人工多能性幹細胞の選別方法

  山中 伸弥; 高橋 和利; 小柳 三千代; 大貫 茉里

  
• 特表2013-528356, 特願2012-556312, ヒト人工多能性幹細胞の選択方法

  山中 伸弥; 高橋 和利; 大貫 茉里

  
• 特表2013-503603, 特願2012-512108, 安全な多能性幹細胞の選択方法

  山中 伸弥; 大貫 茉里

  

External funds: Kakenhi

• Establishment of comprehensive analytical techniques for the search for primate-specific transposable elements responsible for stem cell fate decisions

  Grant-in-Aid for Research Activity Start-up

  0701:Biology at molecular to cellular levels, and related fields

  Kyoto University

  Mari Ohnuki

  From 31 Aug. 2023, To 31 Mar. 2025, Project Closed

  転移因子;霊長類;進化;幹細胞;発生;CRISPR;多能性幹細胞;内在性レトロウイルス;CRISPRスクリーニング;CRISPR-Cas13;転写制御

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K19348/
• ヒト特性に関わるノンコーディング変異の網羅的同定

  Grant-in-Aid for Scientific Research (B)

  Basic Section 43050:Genome biology-related

  Kyoto University

  井上 詞貴

  From 01 Apr. 2024, To 31 Mar. 2027, Granted

  エンハンサー;遺伝子発現制御;MPRA;進化;軟骨細胞;神経前駆細胞;ゲノム進化;ヒト進化

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K02004/

Education

Teaching subject(s)

• From 01 Apr. 2025, To 31 Mar. 2026

  ILAS Seminar

  Z001, Spring, Institute for Liberal Arts and Sciences, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  ILAS Seminar

  Z001, Spring, Institute for Liberal Arts and Sciences, 2