Basic Information

Academic Degree

• 修士（農学）(東京大学)
• 博士（農学）(東京大学)

Research History

• From Apr. 2016, To Present

  Kyoto University, Center for iPS Cell Research and Application (CiRA), Sub-PI
• From 2011, To 2016

  National Institute for Basic Biology, 初期発生研究部門, 助教

ID,URL

• J-Global ID

  201501011629868620

• External link

  https://kaken.nii.ac.jp/d/r/20360597.ja.html

researchmap URL

• https://researchmap.jp/yayoitoy

Research

Research Interests

• iPSC
• 幹細胞
• 転写因子
• 細胞分化
• Trophoblast
• 栄養外胚葉
• ES細胞
• 哺乳類
• 発生
• 初期胚

Research Areas

• Life sciences, Cell biology
• Life sciences, Developmental biology

Papers

• Induction of Functional Mesenchymal Stem/Stromal Cells from Human iPCs Via a Neural Crest Cell Lineage Under Xeno-Free Conditions

  Daisuke Kamiya; Nana Takenaka-Ninagawa; Souta Motoike; Mikihito Kajiya; Teppei Akaboshi; Chengzhu Zhao; Mitsuaki Shibata; Sho Senda; Yayoi Toyooka; Hidetoshi Sakurai; Hidemi Kurihara; Makoto Ikeya

  SSRN Electronic Journal, 2020

  
• Morphological Effects of Protein Kinase C Activators in Mouse Late Morulae.

  Takahisa Noto; Shinnji Maeda; Yayoi Toyooka; Tomohiko Okuda; Miki Sugimoto; Hajime Miyamoto; Noboru Manabe

  Journal of Mammalian Ova Research, 1997

  
• Effects of Transfected Human and Mouse SRY/Sry Genes upon the Expression of Hypothetical SRY-Cascade Genes in Cultured Mouse Sertoli Cell Line, TM-4.

  HIROTA Osamu; NISHINO Koichiro; TOYOOKA Yayoi; KODAMA Terutaka; TANAKA Satoshi; YAMANOUCHI Keitaro; TOJO Hideaki; TACHI Chikashi

  Journal of Reproduction and Development, 1996, Peer-reviewed

  
• Wilms' tumor suppressor gene (WT1) as a target gene of SRY function in a mouse ES cell line transfected with SRY.

  Y Toyooka; S S Tanaka; O Hirota; S Tanaka; N Takagi; K Yamanouchi; H Tojo; C Tachi

  The International journal of developmental biology, Nov. 1998, Peer-reviewed

  
• Generation of pulsatile ERK activity in mouse embryonic stem cells is regulated by Raf activity.

  Yayoi Toyooka; Kazuhiro Aoki; Fumiko Matsukawa Usami; Sanae Oka; Azusa Kato; Toshihiko Fujimori

  Scientific reports, 10 Jun. 2023, Peer-reviewed

  
• Induction of functional xeno-free MSCs from human iPSCs via a neural crest cell lineage.

  Daisuke Kamiya; Nana Takenaka-Ninagawa; Souta Motoike; Mikihito Kajiya; Teppei Akaboshi; Chengzhu Zhao; Mitsuaki Shibata; Sho Senda; Yayoi Toyooka; Hidetoshi Sakurai; Hidemi Kurihara; Makoto Ikeya

  NPJ Regenerative medicine, 15 Sep. 2022, Peer-reviewed

  Access this paper :

  
• Generation of a human SOX10 knock-in reporter iPSC line for visualization of neural crest cell differentiation.

  Teruyoshi Yamashita; Daisuke Kamiya; Nami Kawaraichi; Harufu Toyokawa; Teppei Akaboshi; Makoto Ikeya; Yayoi Toyooka

  Stem cell research, Apr. 2022, Peer-reviewed

  Access this paper :

  
• Generation of human GAPDH knock-in reporter iPSC lines for stable expression of tdTomato in pluripotent and differentiated culture conditions.

  Daisuke Kamiya; Teruyoshi Yamashita; Teppei Akaboshi; Yoshiki Yamaguchi; Yayoi Toyooka; Makoto Ikeya

  Stem cell research, Apr. 2022, Peer-reviewed

  Access this paper :

  
• Pluripotent stem cells in the research for extraembryonic cell differentiation.

  Yayoi Toyooka

  Development, growth & differentiation, Feb. 2021, Peer-reviewed, Invited

  
• Pluripotent stem cells in developmental biology (part 2).

  Makoto Ikeya; Yayoi Toyooka; Mototsugu Eiraku

  Development, growth & differentiation, Feb. 2021

  
• Pluripotent stem cells in developmental biology.

  Makoto Ikeya; Yayoi Toyooka; Mototsugu Eiraku

  Development, growth & differentiation, Jan. 2021

  
• Trophoblast lineage specification in the mammalian preimplantation embryo.

  Yayoi Toyooka

  Reproductive medicine and biology, Jul. 2020, Peer-reviewed, Invited

  
• Early preimplantation cells expressing Cdx2 exhibit plasticity of specification to TE and ICM lineages through positional changes.

  Yayoi Toyooka; Sanae Oka; Toshihiko Fujimori

  Developmental biology, 01 Mar. 2016, Peer-reviewed

  
• Nr0b1 is a negative regulator of Zscan4c in mouse embryonic stem cells.

  Setsuko Fujii; Satomi Nishikawa-Torikai; Yoko Futatsugi; Yayoi Toyooka; Mariko Yamane; Satoshi Ohtsuka; Hitoshi Niwa

  Scientific reports, 16 Mar. 2015, Peer-reviewed

  
• Celsr1 is required for the generation of polarity at multiple levels of the mouse oviduct.

  Dongbo Shi; Kouji Komatsu; Mayumi Hirao; Yayoi Toyooka; Hiroshi Koyama; Fadel Tissir; André M Goffinet; Tadashi Uemura; Toshihiko Fujimori

  Development (Cambridge, England), Dec. 2014, Peer-reviewed

  
• Bre1a, a histone H2B ubiquitin ligase, regulates the cell cycle and differentiation of neural precursor cells.

  Yugo Ishino; Yoshitaka Hayashi; Masae Naruse; Koichi Tomita; Makoto Sanbo; Takahiro Fuchigami; Ryoji Fujiki; Kenzo Hirose; Yayoi Toyooka; Toshihiko Fujimori; Kazuhiro Ikenaka; Seiji Hitoshi

  The Journal of neuroscience : the official journal of the Society for Neuroscience, 19 Feb. 2014, Peer-reviewed

  
• Sall4 is essential for stabilization, but not for pluripotency, of embryonic stem cells by repressing aberrant trophectoderm gene expression.

  Shunsuke Yuri; Sayoko Fujimura; Keisuke Nimura; Naoki Takeda; Yayoi Toyooka; Yu-Ichi Fujimura; Hiroyuki Aburatani; Kiyoe Ura; Haruhiko Koseki; Hitoshi Niwa; Ryuichi Nishinakamura

  Stem cells (Dayton, Ohio), Apr. 2009, Peer-reviewed

  
• Identification and characterization of subpopulations in undifferentiated ES cell culture.

  Yayoi Toyooka; Daisuke Shimosato; Kazuhiro Murakami; Kadue Takahashi; Hitoshi Niwa

  Development (Cambridge, England), Mar. 2008, Peer-reviewed

  
• Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells.

  Shinji Masui; Yuhki Nakatake; Yayoi Toyooka; Daisuke Shimosato; Rika Yagi; Kazue Takahashi; Hitoshi Okochi; Akihiko Okuda; Ryo Matoba; Alexei A Sharov; Minoru S H Ko; Hitoshi Niwa

  Nature cell biology, Jun. 2007, Peer-reviewed

  
• Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation.

  Hitoshi Niwa; Yayoi Toyooka; Daisuke Shimosato; Dan Strumpf; Kadue Takahashi; Rika Yagi; Janet Rossant

  Cell, 02 Dec. 2005, Peer-reviewed

  
• An efficient system to establish multiple embryonic stem cell lines carrying an inducible expression unit.

  Shinji Masui; Daisuke Shimosato; Yayoi Toyooka; Rika Yagi; Kazue Takahashi; Hitoshi Niwa

  Nucleic acids research, 01 Mar. 2005, Peer-reviewed

  
• Embryonic stem cells can form germ cells in vitro.

  Yayoi Toyooka; Naoki Tsunekawa; Ryuko Akasu; Toshiaki Noce

  Proceedings of the National Academy of Sciences of the United States of America, 30 Sep. 2003, Peer-reviewed

  
• Expression and intracellular localization of mouse Vasa-homologue protein during germ cell development.

  Y Toyooka; N Tsunekawa; Y Takahashi; Y Matsui; M Satoh; T Noce

  Mechanisms of development, May 2000, Peer-reviewed

  
• The mouse homolog of Drosophila Vasa is required for the development of male germ cells.

  S S Tanaka; Y Toyooka; R Akasu; Y Katoh-Fukui; Y Nakahara; R Suzuki; M Yokoyama; T Noce

  Genes & development, 01 Apr. 2000, Peer-reviewed

  

Misc.

• Vasa positive cell observed in embryogenesis of Knock-in insertion mutation ES cell.

  豊岡やよい; 田中聡; 福井由宇子; 中原陽子; 横山峯介; 野口美穂; 野瀬俊明

  日本発生生物学会大会発表要旨集, 1998

  
• Possibility of which Wilms tumor inhibiting gene WT1 is one of downstream gene clusters of Sry cascade. Comparative examination on in-vitro and in-vivo.

  豊岡やよい; 田中聡; 山内啓太郎; 東条英昭; 舘ちかし

  日本畜産学会大会講演要旨, 1997

  
• Possibility of expression induction of Wilms’ tumor inhibiting gene (WT1) by mouse Sry. Analysis using in vivo and in vitro culture system.

  豊岡やよい; 田中聡; 山内啓太郎; 東条英昭; 舘ちかし

  日本発生生物学会大会発表要旨集, 1997

  
• On the production of transformed cell line by the foreign derived Sry gene transfer in mouse ES cell line TMA-18 with the karyotype of XX.

  豊岡やよい; 西野光一郎; 広田治; 田中聡; 山内啓太郎; 東条英昭; 舘ちかし

  日本畜産学会大会講演要旨, 1996

  
• Examination of the possibility of the expression control of WT1 gene by the Sry gene using ES cell.

  豊岡やよい; 西野光一郎; 田中聡; 山内啓太郎; 東条英昭; 舘りん

  日本繁殖生物学会講演要旨, 1996

  
• ヒトiPSC由来神経堤細胞の維持拡大培養系の構築

  豊岡やよい; 豊岡やよい; 河原市奈美; 上谷大介; 上谷大介; 小茂池勇作; 小茂池勇作; 山下輝芳; 山下輝芳; 池谷真; 池谷真

  日本再生医療学会総会(Web), 2023

  
• ヒトiPS細胞由来神経堤幹細胞の維持培養系の構築

  豊岡やよい; 河原市奈美; 池谷真

  日本分子生物学会年会プログラム・要旨集(Web), 2020

  
• 未分化ES細胞集団中のsub-populationの性質について

  豊岡やよい; 下里大輔; 荒木良子; 安倍真澄; 丹羽仁史

  日本分子生物学会年会講演要旨集, 2005

  
• 未分化(Oct-3/4陽性)ES細胞集団中に存在するsub-populationについて

  豊岡やよい; 下里大輔; 荒木良子; 安倍真澄; 丹羽仁史

  日本発生生物学会大会発表要旨集, 2005

  
• 栄養外はい葉分化に関わる転写因子の機能解析 2004

  丹羽仁史; 高橋一恵; 矢木利香; 豊岡やよい

  日本発生生物学会大会発表要旨集, 2004

  
• ES細胞の培養系における生殖系列細胞の分化

  豊岡やよい; 恒川直樹; 野瀬俊明

  日本獣医学会学術集会講演要旨集, 2003

  
• 発生・再生を支える幹細胞システム ES細胞のin vitro分化誘導におけるVasa陽性生殖細胞の出現

  豊岡 やよい; 小川 峰太郎; 西川 伸一; 田中 智; Rossant Janet; 福井 由宇子; 中原 陽子; 田中 聡; 野瀬 俊明

  日本発生生物学会大会講演要旨集, May 2000

  
• ES細胞のin vitro分化系におけるVasa陽性細胞の出現について

  豊岡 やよい; 田中 聡; 小川 峰太郎; 西川 伸一; 福井 由宇子; 中原 陽子; 横山 峯介; 野瀬 俊明

  日本発生生物学会大会講演要旨集, May 1999

  
• Forced expression of Tbx3 promotes LIF-independent self-renewal of mouse ES cells

  Kazuya Ogawa; Daisuke Shimosato; Kadue Takahashi; Rika Yagi; Yayoi Toyooka; Shinji Masui; R. Matoba; Minoru S. Ko

  DEVELOPMENTAL BIOLOGY, Jun. 2007

  
• ヒトiPS細胞由来神経堤細胞の維持拡大培養系の構築

  豊岡 やよい; 河原市 奈美; 池谷 真

  末梢神経, Dec. 2022

  
• ヒトiPS細胞からの腸管神経前駆細胞誘導法及び拡大培養法の構築

  山下 輝芳; 日吉 秀行; 武 和巳; 小茂池 勇作; 上谷 大介; 豊岡 やよい; 松本 寛和; 池谷 真

  末梢神経, Dec. 2022

  
• 胚性幹細胞 ES細胞を用いた栄養外胚葉分化の研究—第5土曜特集 再生医学--臨床と研究の最前線 ; 基礎編

  豊岡 やよい; 丹羽 仁史

  医学のあゆみ, 29 Apr. 2006

  
• ES細胞の未分化性の維持—再生医学 ; ES細胞

  豊岡 やよい; 丹羽 仁史

  Molecular medicine = モレキュラーメディシン : 分子医学を臨床へ, 2003

  
• ES細胞から生殖系列細胞への分化制御—特集 ES細胞の分化制御と再生医学

  豊岡 やよい; 野瀬 俊明

  細胞工学 = Cell technology, Jul. 2001

  
• 幹細胞ワールド 再生医学への道 急速に進む基礎研究と医学応用の現状 はい性幹細胞と生殖系列細胞

  野瀬俊明; 豊岡やよい

  実験医学, 2001

  

Books and Other Publications

• 動物遺伝育種学実験法

  佐々木, 義之

  朝倉書店, Mar. 1998

  Find Kyoto University's collection of books :

  
• 細胞培養・組織培養の技術

  日本組織培養学会

  朝倉書店, Jul. 2023

  Find Kyoto University's collection of books :

  

Industrial Property Rights

• 特許第7330466号, JP2018043949, 細胞の培養方法

  池谷 真; 上谷(豊岡) やよい

  
• WO2019-107485, JP2018043949, 細胞の培養方法

  池谷 真; 上谷(豊岡) やよい

  
• 特許第7341433号, JP2019032543, 腸管神経前駆細胞の製造方法

  池谷 真; 上谷(豊岡) やよい; 上谷 大介; 山下 輝芳; 武 和巳

  
• WO2020-040166, JP2019032543, 腸管神経前駆細胞の製造方法

  池谷 真; 上谷(豊岡) やよい; 上谷 大介; 山下 輝芳; 武 和巳

  
• 特開2002-065261, 特願2000-260449, 生殖細胞の取得方法

  野瀬 俊明; 豊岡 やよい

  

External funds: Kakenhi

• Analysis of Cdx2 gene expression patterns and cell movement in mouse preimplantation embryos by live-cell imaging

  Grant-in-Aid for Young Scientists (B)

  National Institute for Basic Biology

  Yayoi TOYOOKA

  From 01 Apr. 2011, To 31 Mar. 2013, Project Closed

  細胞分化;栄養外胚葉;転写因子;Trophoblast

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770259/
• Behavioral analyses of genes and cells in early mammalian development

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Biological Sciences

  National Institute for Basic Biology

  Toshihiko FUJIMORI

  From 01 Apr. 2009, To 31 Mar. 2015, Project Closed

  哺乳類;初期発生;細胞分化;ライブイメージング;遺伝子発現;初期胚;発生;細胞

  https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-21116005/
• Oct-3/4およびRex-1遺伝子発現を指標とした成体多能性幹細胞の局在観察

  Grant-in-Aid for Young Scientists (B)

  The Institute of Physical and Chemical Research

  豊岡 やよい

  From 01 Apr. 2004, To 31 Mar. 2006, Project Closed

  成体幹細胞;ES細胞;Oct-3;4遺伝子;MAPC;4;Rex-1;成体多能性幹細胞

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16770177/
• 細胞系譜に着目したiPS細胞由来間葉系幹細胞の基盤的研究

  Grant-in-Aid for Scientific Research (B)

  Basic Section 56020:Orthopedics-related

  Kyoto University

  池谷 真

  From 01 Apr. 2023, To 31 Mar. 2026, Granted

  間葉系幹細胞;発生系譜

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23H03028/
• 細胞系譜に着目したiPS細胞由来間葉系幹細胞の基盤的研究

  Grant-in-Aid for Scientific Research (B)

  Basic Section 56020:Orthopedics-related

  Kyoto University

  池谷 真

  From 01 Apr. 2023, To 31 Mar. 2026, Granted

  間葉系幹細胞;細胞系譜;発生系譜

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K27719/

Administration

Faculty management (title, position)

• From 01 Jan. 2018

  T-CiRAグループ長