Basic Information

Academic Degree

• 修士（医科学）(信州大学)
• 博士（医学）(京都大学)

ID,URL

• J-Global ID

  201601004056330932

researchmap URL

• https://researchmap.jp/7000015503

Research

Research Topics, Overview of the research

• Research Topics

  Development of kidney regeneration using induced pluripotent stem cells

• Overview of the research

  The total number of patients receiving dialysis therapy due to end-stage chronic renal failure continues to increase every year. Effective treatment for renal diseases has not been established yet, coupled with the aging society, it is expected that dialysis medical expenses will continue to increase. On the other hand, research to induce major organs from pluripotent stem cells has been actively conducted in recent years. Therefore, I am developing a method to induce renal cells from iPS cells, elucidating the signal molecules and mechanisms involved in renal development, and developing a novel pathological model for intractable hereditary renal diseases.

Papers

• Differentiation and isolation of iPSC-derived remodeling ductal plate-like cells by use of an AQP1-GFP reporter human iPSC line.

  Matsui S; Ochiai M; Yasuda K; Mae SI; Kotaka M; Toyoda T; Yamamoto T; Osafune K

  Stem cell research, Mar. 2019, Peer-reviewed

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• [Dissecting early development of the kidney by single cell transcriptomics].

  Sakamoto S; Mae SI; Osafune K; Okada C; Kabai R; Watanabe A

  Nihon yakurigaku zasshi. Folia pharmacologica Japonica, 2019, Peer-reviewed

  
• Protocol to Generate Ureteric Bud Structures from Human iPS Cells.

  Mae SI; Ryosaka M; Osafune K

  Methods in molecular biology (Clifton, N.J.), 2019, Peer-reviewed

  
• Development of new method to enrich human iPSC-derived renal progenitors using cell surface markers.

  Hoshina A; Kawamoto T; Sueta SI; Mae SI; Araoka T; Tanaka H; Sato Y; Yamagishi Y; Osafune K

  Scientific Reports, Apr. 2018, Peer-reviewed

  
• Generation of branching ureteric bud tissues from human pluripotent stem cells

  Shin-Ichi Mae; Makoto Ryosaka; Taro Toyoda; Kyoko Matsuse; Yoichi Oshima; Hiraku Tsujimoto; Shiori Okumura; Aya Shibasaki; Kenji Osafune

  Biochemical and Biophysical Research Communications, 01 Jan. 2018, Peer-reviewed

  
• Human pluripotent stem cell-derived erythropoietin-producing cells ameliorate renal anemia in mice

  Hitomi H; Kasahara T; Katagiri N; Hoshina A; Mae SI; Kotaka M; Toyohara T; Rahman A; Nakano D; Niwa A; Saito MK; Nakahata T; Nishiyama A; Osafune K

  Science Translational Medicine, Sep. 2017, Peer-reviewed

  
• Transcriptional Analysis of Intravenous Immunoglobulin Resistance in Kawasaki Disease Using an Induced Pluripotent Stem Cell Disease Model

  Kazuyuki Ikeda; Yasutaka Mizoro; Tomonaga Ameku; Yui Nomiya; Shin-Ichi Mae; Satoshi Matsui; Yuki Kuchitsu; Chinatsu Suzuki; Akiko Hamaoka-Okamoto; Tomoyo Yahata; Masakatsu Sone; Keisuke Okita; Akira Watanabe; Kenji Osafune; Kenji Hamaoka

  CIRCULATION JOURNAL, Jan. 2017, Peer-reviewed

  
• Redefining definitive endoderm subtypes by robust induction of human induced pluripotent stem cells

  Kunihiko Matsuno; Shin-Ichi Mae; Chihiro Okada; Masahiro Nakamura; Akira Watanabe; Taro Toyoda; Eiji Uchida; Kenji Osafune

  DIFFERENTIATION, Dec. 2016, Peer-reviewed

  
• Identification of MMP1 as a novel risk factor for intracranial aneurysms in ADPKD using iPSC models

  Tomonaga Ameku; Daisuke Taura; Masakatsu Sone; Tomohiro Numata; Masahiro Nakamura; Fumihiko Shiota; Taro Toyoda; Satoshi Matsui; Toshikazu Araoka; Tetsuhiko Yasuno; Shin-Ichi Mae; Hatasu Kobayashi; Naoya Kondo; Fumiyo Kitaoka; Naoki Amano; Sayaka Arai; Tomoko Ichisaka; Norio Matsuura; Sumiko Inoue; Takuya Yamamoto; Kazutoshi Takahashi; Isao Asaka; Yasuhiro Yamada; Yoshifumi Ubara; Eri Muso; Atsushi Fukatsu; Akira Watanabe; Yasunori Sato; Tatsutoshi Nakahata; Yasuo Mori; Akio Koizumi; Kazuwa Nakao; Shinya Yamanaka; Kenji Osafune

  SCIENTIFIC REPORTS, Jul. 2016, Peer-reviewed

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• Cell Therapy Using Human Induced Pluripotent Stem Cell-Derived Renal Progenitors Ameliorates Acute Kidney Injury in Mice

  Takafumi Toyohara; Shin-lchi Mae; Shin-lchi Sueta; Tatsuyuki Inoue; Yukiko Yamagishi; Tatsuya Kawamoto; Tomoko Kasahara; Azusa Hoshina; Taro Toyoda; Hiromi Tanaka; Toshikazu Araoka; Aiko Sato-Otsubo; Kazutoshi Takahashi; Yasunori Sato; Noboru Yamaji; Seishi Ogawa; Shinya Yamanaka; Kenji Osafune

  STEM CELLS TRANSLATIONAL MEDICINE, Sep. 2015, Peer-reviewed

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• Kidney regeneration from human iPSCs.

  Mae S; Osafune K

  Current Opinion in Organ Transplantation, Apr. 2015, Peer-reviewed

  
• Cell aggregation optimizes the differentiation of human ESCs and iPSCs into pancreatic bud-like progenitor cells

  Taro Toyoda; Shin-Ichi Mae; Hiromi Tanaka; Yasushi Kondo; Michinori Funato; Yoshiya Hosokawa; Tomomi Sudo; Yoshiya Kawaguchi; Kenji Osafune

  STEM CELL RESEARCH, Mar. 2015, Peer-reviewed

  
• Generation of Alveolar Epithelial Spheroids via Isolated Progenitor Cells from Human Pluripotent Stem Cells

  Shimpei Gotoh; Isao Ito; Tadao Nagasaki; Yuki Yamamoto; Satoshi Konishi; Yohei Korogi; Hisako Matsumoto; Shigeo Muro; Toyohiro Hirai; Michinori Funato; Shin-Ichi Mae; Taro Toyoda; Aiko Sato-Otsubo; Seishi Ogawa; Kenji Osafune; Michiaki Mishima

  STEM CELL REPORTS, Sep. 2014, Peer-reviewed

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• Efficient and Rapid Induction of Human iPSCs/ESCs into Nephrogenic Intermediate Mesoderm Using Small Molecule-Based Differentiation Methods

  Toshikazu Araoka; Shin-ichi Mae; Yuko Kurose; Motonari Uesugi; Akira Ohta; Shinya Yamanaka; Kenji Osafune

  PLOS ONE, Jan. 2014, Peer-reviewed

  
• Monitoring and robust induction of nephrogenic intermediate mesoderm from human pluripotent stem cells

  Shin-Ichi Mae; Akemi Shono; Fumihiko Shiota; Tetsuhiko Yasuno; Masatoshi Kajiwara; Nanaka Gotoda-Nishimura; Sayaka Arai; Aiko Sato-Otubo; Taro Toyoda; Kazutoshi Takahashi; Naoki Nakayama; Chad A. Cowan; Takashi Aoi; Seishi Ogawa; Andrew P. McMahon; Shinya Yamanaka; Kenji Osafune

  Nature Communications, 2013, Peer-reviewed

  

Presentations

• ヒトiPS細胞から三次元構造を有する後腎尿管芽への分化誘導法の確立

  前伸一; 豊田太郎; 荒岡利和; 塩田文彦; 豊原敬文; 笠原朋子; 小川誠司; 山中伸弥; 長船健二

  第56回日本腎臓学会学術総会, 10 May 2013

  
• ヒトiPS細胞から三次元構造を有する後腎尿管芽への分化誘導法の確立

  前伸一; 豊田太郎; 荒岡利和; 塩田文彦; 豊原敬文; 笠原朋子; 小川誠司; 山中伸弥; 長船健二

  第12回日本再生医療学会総会, 22 Mar. 2013

  
• ヒトiPS細胞から腎構成細胞に分化しうる中間中胚葉への高効率分化誘導法の確立

  前伸一; 山中伸弥; 長船健二

  第3回分子腎臓フォーラム, 01 Sep. 2012

  
• Monitoring and robust induction of intermediate mesoderm from human iPSCs and ESCs

  Shin-Ichi Mae; Akemi Shono; Fumihiko Shiota; Tetsuhiko Yasuno; Masatoshi Kajiwara; Nanaka Gotoda-Nishimura; Sayaka Arai; Aiko Sato-Otubo; Taro Toyoda; Kazutoshi Takahashi; Naoki Nakayama; Chad A. Cowan; Takashi Aoi; Seishi Ogawa; Andrew P. McMahon; Shinya Yamanaka; Kenji Osafune

  ISSCR 10th Annual Meeting, 14 Jun. 2012

  
• ヒトiPS細胞から腎構成細胞に分化しうる中間中胚葉への高効率分化誘導法の確立

  前伸一; 庄野朱美; 塩田文彦; 小川誠司; Andrew; P. McMahon; 山中伸弥; 長船健二

  第55回日本腎臓学会学術総会, 03 Jun. 2012

  
• ヒト多能性幹細胞から腎臓系譜に分化する中間中胚葉細胞の高効率な分化誘導法の確立

  前伸一; 長船健二

  日本組織培養学会第85回大会, 17 May 2012

  
• Induction and monitoring of intermediate mesoderm from human iPSCs and ESCs

  Shin-Ichi Mae; Akemi Shono; Fumihiko Shiota; Tetsuhiko Yasuno; Nanaka Nishimura; Sayaka Arai; Kazutoshi Takahashi; Aiko Matsubara; Naoki Nakayama; Seishi Ogawa; Andrew P. McMahon; Shinya Yamanaka; Kenji Osafune

  ISSCR 9th Annual Meeting, 15 Jun. 2011

  

Books and Other Publications

• Current Therapy 2018 Vol.36 No.3

  奥村詩織; 前伸一; 長船健二, Contributor, 腎臓病患者由来iPS細胞の活用

  株式会社ライフメディコム, 2018, Not refereed

  
• 医学のあゆみ 252(2)

  前 伸一; 長船健二, Contributor, iPS 細胞を用いた腎疾患に対する再生医療開発

  医歯薬出版, 2015, Not refereed

  
• Nephrology Frontier 14(2)

  前 伸一, Contributor, 腎疾患患者由来iPS 細胞の臨床活用

  メディカルレビュー社, 2015, Not refereed

  
• 医学のあゆみ 248(2)

  笠原朋子; 前伸一; 長船健二, Contributor, iPS 細胞を用いた腎臓再生研究の進展

  医歯薬出版, 2014, Not refereed

  
• iPS 細胞研究最前線

  前伸一; 長船健二, Contributor, 腎疾患に対する再生医療開発

  医歯薬出版, 2014, Not refereed

  
• 遺伝子

  前伸一; 沖田圭介; 長船健二, Contributor, iPS 細胞への遺伝子導入時における適切なプロトコール

  技術情報協会, 2014, Not refereed

  
• 分子腎臓病学実験操作法

  前伸一; 荒岡利和; 長船健二, Contributor, iPS 細胞培養法

  中外医学社, 2013, Not refereed

  

Industrial Property Rights

• 特願62/587693, Method for generating Ureteric Bud-like Tissues

  Osafune K; Mae S; Ryosaka M

  

Awards

• 18 May 2012

  日本組織培養学会, 日本組織培養学会第85 回大会 奨励賞
• 15 Jun. 2011

  International Society for Stem cell Research (ISSCR), The 9th International Society for Stem cell Research (ISSCR) Travel Award
• 01 Sep. 2012

  分子腎臓フォーラム, 第3回分子腎臓フォーラム優秀賞
• 10 May 2013

  日本腎臓学会, 第56回日本腎臓学会学術総会優秀演題賞

External funds: Kakenhi

• Expansion of human iPS cell-derived ureteric bud organoids

  Grant-in-Aid for Scientific Research (C)

  Basic Section 53040:Nephrology-related

  Kyoto University

  Shin-Ichi Mae

  From 01 Apr. 2019, To 31 Mar. 2022, Project Closed

  iPS細胞;尿管芽;腎臓;拡大培養;ヒトiPS細胞;オルガノイド

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K08703/
• Development of a novel pathological model for intractable hereditary kidney disease using human iPS cells

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  Shin-ichi Mae

  From 01 Apr. 2017, To 31 Mar. 2019, Project Closed

  ヒトiPS細胞;常染色体優性多発性嚢胞腎;尿管芽;腎集合管;iPS細胞

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K15546/
• Induction of human iPS cells to primitive streak that can differentiate into kidney

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  Shin-ichi Mae

  From 01 Apr. 2015, To 31 Mar. 2017, Project Closed

  ヒトiPS細胞;原始線条;腎臓

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K19453/
• 時空間的な腎発生過程を模倣した成熟集合管の作製

  Grant-in-Aid for Scientific Research (C)

  Basic Section 44020:Developmental biology-related

  Kyoto University

  前 伸一

  From 01 Apr. 2022, To 31 Mar. 2025, Granted

  腎臓;集合管;iPS細胞

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K06236/