Basic Information

Faculty

• Faculty of Agriculture

Email Address

• Email Address

  tsuji.satsuki.5nkyoto-u.ac.jp

Professional Memberships

• From Sep. 2023, To Present

  日本魚類学会
• From Mar. 2014, To Present

  日本生態学会

Academic Degree

• 18 Mar. 2016

  龍谷大学修士（工学）
• 15 Mar. 2019

  龍谷大学博士（工学）

Academic Resume (Graduate Schools)

• 龍谷大学, 大学院理工学研究科環境ソリューション工学専攻博士後期課程, 修了
• 龍谷大学, 大学院理工学研究科環境ソリューション工学専攻修士課程, 修了

Academic Resume (Undergraduate School/Majors)

• 龍谷大学, 理工学部環境ソリューション工学科, 卒業

Research History

• From Apr. 2023, To Present

  京都大学大学院情報学研究科, 生物圏情報学講座
• From Apr. 2021, To Mar. 2023

  京都大学大学院理学研究科, 日本学術振興会 特別研究員PD
• From Apr. 2020, To Mar. 2021

  山口大学環境DNA研究センター, 学術研究員
• From Apr. 2019, To Mar. 2020

  日本学術振興会特別研究員 PD
• From Apr. 2018, To Mar. 2019

  日本学術振興会特別研究員 DC2

ID,URL

• ORCID ID

  0000-0003-0963-6200

• J-Global ID

  202001004695615614

• External link

  https://sites.google.com/view/satsuki-tsuji425/home

researchmap URL

• https://researchmap.jp/t-satsuki

Research

Research Interests

• 保全遺伝学
• 水域生態学
• 環境DNA分析

Papers

• Identifying spawning events in fish by observing a spike in environmental DNA concentration after spawning

  Satsuki Tsuji; Naoki Shibata

  Environmental DNA, 01 Jan. 2021

  
• Rapid assessment of invasion front and biological impact of the invasive fish Coreoperca herzi using quantitative eDNA metabarcoding

  Satsuki Tsuji; Hideyuki Doi; Yusuke Hibino; Naoki Shibata; Katsutoshi Watanabe

  Biological Invasions, 12 Jun. 2024, Peer-reviewed, Lead author, Corresponding author

  
• 江の川浜原ダム下流における置土が河川環境に与える影響

  花岡拓身; 齋藤稔; 赤松良久; 宮園誠二; 中尾遼平; 辻冴月; 小林勘太

  土木学会論文集B1（水工学）, Dec. 2021, Peer-reviewed

  
• 環境DNA定量メタバーコーディング法を用いた江の川土師ダム下流における魚類多様性の把握

  宮園誠二; 児玉貴央; 赤松良久; 中尾遼平; 辻冴月; 宮平秀明

  河川技術論文集 第28巻, Jun. 2022, Peer-reviewed

  
• 環境DNA定量メタバ―コーディング法による堰が河川魚類群集構造に及ぼす影響の評価

  宮園誠二; 児玉貴央; 赤松良久; 辻冴月; 中尾遼平

  河川技術論文集, Jun. 2021, Peer-reviewed

  
• A COMPARATIVE STUDY OF THE NATIONAL CENSUS ON RIVER ENVIRONMENTS AND THE QUANTITATIVEeDNA METABARCODING ANALYSIS

  辻冴月; 赤松良久; 中尾遼平; 都築隆禎; 内藤太輔; 横山良太; 畔上正樹; 乾隆帝

  Advances in River Engineering, Jun. 2021, Peer-reviewed, Lead author, Corresponding author

  
• ASSESSMENT ON THE SEASONAL BIOMASS VARIATIONS OF RIVERINE ORGANISMS USING ENVIRONMENTAL DNA ANALYSIS IN THE TAKATSU RIVER, WESTERN JAPAN

  KOBAYASHI Kanta; SAITO Minoru; AKAMATSU Yoshihisa; INUI Ryutei; KONO Takanori; TSUJI Satsuki; NAKAO Ryohei

  Journal of Japan Society of Civil Engineers, Ser. B1 (Hydraulic Engineering), Oct. 2020, Peer-reviewed

  
• Environmental DNA phylogeography: Successful reconstruction of phylogeographic patterns of multiple fish species from cups of water

  Satsuki Tsuji; Naoki Shibata; Ryutei Inui; Ryohei Nakao; Yoshihisa Akamatsu; Katsutoshi Watanabe

  Molecular Ecology Resources, Mar. 2023, Peer-reviewed, Lead author, Corresponding author

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• Quantitative environmental DNA metabarcoding shows high potential as a novel approach to quantitatively assess fish community

  Satsuki Tsuji; Ryutei Inui; Ryohei Nakao; Seiji Miyazono; Minoru Saito; Takanori Kono; Yoshihisa Akamatsu

  Scientific Reports, 13 Dec. 2022, Peer-reviewed, Lead author, Corresponding author

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• Differences in the genetic structure between and within two landlocked Ayu groups with different migration patterns in Lake Biwa revealed by environmental DNA analysis

  Satsuki Tsuji; Naoki Shibata; Hayato Sawada; Katsutoshi Watanabe

  Environmental DNA, 15 Aug. 2022, Peer-reviewed, Lead author, Corresponding author

  
• Comparative study on nuclear and mitochondrial DNA of Ayu Plecoglossus altivelis for environmental DNA-based spawning evaluation

  Minoru Saito; Satsuki Tsuji; Ryohei Nakao; Seiji Miyazono; Yoshihisa Akamatsu

  Landscape and Ecological Engineering, 29 Jul. 2022, Peer-reviewed

  
• Analysis of the Persistence and Particle Size Distributional Shift of Sperm-Derived Environmental DNA to Monitor Jack Mackerel Spawning Activity

  Satsuki Tsuji; Hiroaki Murakami; Reiji Masuda

  Environmental Science & Technology, 22 Jul. 2022, Peer-reviewed, Lead author, Corresponding author

  
• Abundance estimation of riverine macrophyte Egeria densa using environmental DNA: effects of sampling season and location

  Takao Kodama; Seiji Miyazono; Yoshihisa Akamatsu; Satsuki Tsuji; Ryohei Nakao

  Limnology, Apr. 2022, Peer-reviewed

  
• Pre-centrifugation before DNA extraction mitigates extraction efficiency reduction of environmental DNA caused by the preservative solution (benzalkonium chloride) remaining in the filters

  Satsuki Tsuji; Ryohei Nakao; Minoru Saito; Toshifumi Minamoto; Yoshihisa Akamatsu

  Limnology, Jan. 2022, Peer-reviewed, Lead author, Corresponding author

  
• Evaluation of the tributaries as habitats for Ayu in the Gonokawa River using environmental DNA analysis.

  Seiji MIYAZONO; Takao KODAMA; Yoshihisa AKAMATSU; Ryohei NAKAO; Minoru SAITO; Satsuki TSUJI

  Ecology and Civil Engineering, 10 Dec. 2021, Peer-reviewed

  
• EXAMINING ENVIRONMENTAL FACTORS AFFECTING FISH ASSEMBLAGE PATTERNS IN THE TRIBUTARIES USING QUANTITATIVE EDNA METABARCODING

  Seiji MIYAZONO; Takao KODAMA; Yoshihisa AKAMATSU; Ryohei NAKAO; Satsuki TSUJI; Hideaki MIYAHIRA

  Journal of Japan Society of Civil Engineers, Ser. B1 (Hydraulic Engineering), Dec. 2021, Peer-reviewed

  
• Compilation of real‐time PCR conditions toward the standardization of environmental DNA methods

  Hideyuki Doi; Toshifumi Minamoto; Teruhiko Takahara; Satsuki Tsuji; Kimiko Uchii; Satoshi Yamamoto; Izumi Katano; Hiroki Yamanaka

  Ecological Research, May 2021, Peer-reviewed

  
• EFFECTS OF SEDIMENT REPLENISHMENT ON THE RIVERINE ENVIRONMENT IN THE DOWNSTREAM REACHES OF THE HAMAHARA DAM, GONOKAWA RIVER, WESTERN JAPAN

  Takumi HANAOKA; Minoru SAITO; Yoshihisa AKAMATSU; Seiji MIYAZONO; Ryohei NAKAO; Satsuki TSUJI; Kanta KOBAYASHI

  Journal of Japan Society of Civil Engineers, Ser. B1 (Hydraulic Engineering), 2021, Peer-reviewed

  
• Environmental DNA analysis provides an overview of distribution patterns of two dojo loach species within the Naka-ikemi Wetland, Fukui Prefecture, Japan

  Ryuya Okada; Satsuki Tsuji; Naoki Shibata; Keigo Morita; Tadao Kitagawa; Hiroki Yamanaka

  Ichthyological Research, Jan. 2021, Peer-reviewed, Lead author

  
• Quantitative evaluation of intraspecific genetic diversity in a natural fish population using environmental DNA analysis

  Satsuki Tsuji; Naoki Shibata; Hayato Sawada; Masayuki Ushio

  MOLECULAR ECOLOGY RESOURCES, Sep. 2020, Peer-reviewed, Lead author, Corresponding author

  
• Environmental DNA analysis shows high potential as a tool for estimating intraspecific genetic diversity in a wild fish population

  TSUJI Satsuki; MARUYAMA Atsushi; MIYA Masaki; USHIO Masayuki; SATO Hirotoshi; MINAMOTO Toshifumi; YAMANAKA Hiroki

  Molecular Ecology Resources, Apr. 2020, Peer-reviewed, Lead author

  
• Evaluating intraspecific genetic diversity using environmental DNA and denoising approach: A case study using tank water

  Satsuki Tsuji; Masaki Miya; Masayuki Ushio; Hirotoshi Sato; Toshifumi Minamoto; Hiroki Yamanaka

  Environmental DNA, Jan. 2020, Peer-reviewed, Lead author

  
• The detection of aquatic macroorganisms using environmental DNA analysis—A review of methods for collection, extraction, and detection

  Satsuki Tsuji; Teruhiko Takahara; Hideyuki Doi; Naoki Shibata; Hiroki Yamanaka

  Environmental DNA, 20 Jul. 2019, Peer-reviewed, Lead author

  
• Habitat selection and migration of the common shrimp, Palaemon paucidens in Lake Biwa, Japan—An eDNA‐based study

  Qianqian Wu; Ken Kawano; Toshiyuki Ishikawa; Masayuki K. Sakata; Ryohei Nakao; Masayoshi K. Hiraiwa; Satsuki Tsuji; Hiroki Yamanaka; Toshifumi Minamoto

  Environmental DNA, May 2019, Peer-reviewed

  
• Real-time multiplex PCR for simultaneous detection of multiple species from environmental DNA: an application on two Japanese medaka species

  Satsuki Tsuji; Yuka Iguchi; Naoki Shibata; Iori Teramura; Tadao Kitagawa; Hiroki Yamanaka

  SCIENTIFIC REPORTS, Jun. 2018, Peer-reviewed, Lead author

  
• Environmental DNA reveals nonmigratory individuals of Palaemon paucidens overwintering in Lake Biwa shallow waters

  Qianqian Wu; Ken Kawano; Yoshitoshi Uehara; Noboru Okuda; Masamichi Hongo; Satsuki Tsuji; Hiroki Yamanaka; Toshifumi Minamoto

  Freshwater Science, Jun. 2018, Peer-reviewed

  
• A simple method for preserving environmental DNA in water samples at ambient temperature by addition of cationic surfactant

  Hiroki Yamanaka; Toshifumi Minamoto; Junichi Matsuura; Sho Sakurai; Satsuki Tsuji; Hiromu Motozawa; Masamichi Hongo; Yuki Sogo; Naoki Kakimi; Iori Teramura; Masaki Sugita; Miki Baba; Akihiro Kondo

  LIMNOLOGY, Apr. 2017, Peer-reviewed

  
• Water temperature-dependent degradation of environmental DNA and its relation to bacterial abundance

  Satsuki Tsuji; Masayuki Ushio; Sho Sakurai; Toshifumi Minamoto; Hiroki Yamanaka

  PLOS ONE, Apr. 2017, Peer-reviewed, Lead author

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• Nuclear internal transcribed spacer-1 as a sensitive genetic marker for environmental DNA studies in common carp Cyprinus carpio

  Toshifumi Minamoto; Kimiko Uchii; Teruhiko Takahara; Takumi Kitayoshi; Satsuki Tsuji; Hiroki Yamanaka; Hideyuki Doi

  MOLECULAR ECOLOGY RESOURCES, Mar. 2017, Peer-reviewed

  
• Effects of water pH and proteinase K treatment on the yield of environmental DNA from water samples

  Satsuki Tsuji; Hiroki Yamanaka; Toshifumi Minamoto

  LIMNOLOGY, Jan. 2017, Peer-reviewed, Lead author

  
• On-site filtration of water samples for environmental DNA analysis to avoid DNA degradation during transportation

  Hiroki Yamanaka; Hiromu Motozawa; Satsuki Tsuji; Ryohei C. Miyazawa; Teruhiko Takahara; Toshifumi Minamoto

  ECOLOGICAL RESEARCH, Nov. 2016, Peer-reviewed

  
• Environmental DNA as a 'Snapshot' of Fish Distribution: A Case Study of Japanese Jack Mackerel in Maizuru Bay, Sea of Japan (vol 11, e0149786, 2016)

  Satoshi Yamamoto; Kenji Minami; Keiichi Fukaya; Kohji Takahashi; Hideki Sawada; Hiroaki Murakami; Satsuki Tsuji; Hiroki Hashizume; Shou Kubonaga; Tomoya Horiuchi; Masamichi Hongo; Jo Nishida; Yuta Okugawa; Ayaka Fujiwara; Miho Fukuda; Shunsuke Hidaka; Keita W. Suzuki; Masaki Miya; Hitoshi Araki; Hiroki Yamanaka; Atsushi Maruyama; Kazushi Miyashita; Reiji Masuda; Toshifumi Minamoto; Michio Kondoh

  PLOS ONE, Apr. 2016, Peer-reviewed

  
• Environmental DNA as a 'Snapshot' of Fish Distribution: A Case Study of Japanese Jack Mackerel in Maizuru Bay, Sea of Japan

  Satoshi Yamamoto; Kenji Minami; Keiichi Fukaya; Kohji Takahashi; Hideki Sawada; Hiroaki Murakami; Satsuki Tsuji; Hiroki Hashizume; Shou Kubonaga; Tomoya Horiuchi; Masamichi Hongo; Jo Nishida; Yuta Okugawa; Ayaka Fujiwara; Miho Fukuda; Shunsuke Hidaka; Keita W. Suzuki; Masaki Miya; Hitoshi Araki; Hiroki Yamanaka; Atsushi Maruyama; Kazushi Miyashita; Reiji Masuda; Toshifumi Minamoto; Michio Kondoh

  PLOS ONE, Mar. 2016, Peer-reviewed

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Books and Other Publications

• 誰でも再現できるNGS「前」サンプル調製プロトコール : 生物種別DNA、RNA、クロマチン、シングルセル調製の極意

  鹿島, 誠; 伊藤, 佑; 尾崎, 遼, 第1章5 水圏における環境DNA分析, 第3章14 水圏の環境DNA

  羊土社, Jul. 2024

  Find Kyoto University's collection of books :

  
• 環境DNA: 生態系の真の姿を読み解く

  環境DNA学会; 土居, 秀幸; 近藤, 倫生, 第7章 環境DNA技術の未来

  共立出版, 13 Mar. 2021

  Find Kyoto University's collection of books :

  

Awards

• 20 Aug. 2021

  エスペック環境研究奨励賞
• 19 Mar. 2015

  第６２回日本生態学会鹿児島大会ポスター発表優秀賞
• 30 Sep. 2019

  Limnology Excellent Paper Award（日本陸水学会）
• 03 Jun. 2017

  第２３回日本生態学会近畿地区会奨励賞
• 22 Sep. 2022

  ３学会合同大会つくば大会口頭発表優秀賞（日本緑化工学会・日本景観生態学会）
• 03 Nov. 2019

  第２回環境DNA学会神戸大会ポスター発表最優秀賞

External funds: Kakenhi

• 環境DNA分析に基づく外来種による群集・遺伝子レベルでの影響評価手法の開発と実践

  Grant-in-Aid for Early-Career Scientists

  Basic Section 39060:Conservation of biological resources-related

  Kyoto University

  辻 冴月

  From 01 Apr. 2023, To 31 Mar. 2027, Granted

  外来種;環境DNA;群集;遺伝的かく乱

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K13967/
• Development of a method for monitoring fish spawning events using environmental DNA

  Grant-in-Aid for Early-Career Scientists

  Basic Section 40030:Aquatic bioproduction science-related

  Kyoto University;Yamaguchi University

  Satsuki Tsuji

  From 01 Apr. 2020, To 31 Mar. 2023, Project Closed

  環境DNA;産卵;精子;マアジ;アユ;舞鶴湾;産卵行動;地先産卵

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K15578/
• 検出誤差に対して頑健な環境DNAメタバーコーディングの進展

  Grant-in-Aid for Scientific Research (B)

  Basic Section 39060:Conservation of biological resources-related

  National Institute for Environmental Studies

  深谷 肇一

  From 01 Apr. 2023, To 31 Mar. 2027, Granted

  環境DNAメタバーコーディング;サイト占有モデル;群集動態;遺伝的多様性

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23H02240/
• 検出誤差に対して頑健な環境DNAメタバーコーディングの進展

  Grant-in-Aid for Scientific Research (B)

  Basic Section 39060:Conservation of biological resources-related

  National Institute for Environmental Studies

  深谷 肇一

  From 01 Apr. 2023, To 31 Mar. 2027, Granted

  環境DNAメタバーコーディング;サイト占有モデル;群集動態;遺伝的多様性

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K26933/

Education

Teaching subject(s)

• From 01 Apr. 2024, To 31 Mar. 2025

  Laboratory Course in Animal Science II

  5363, Fall, Faculty of Agriculture, 6
• From 01 Apr. 2024, To 31 Mar. 2025

  Bioresource and Environmental Informatics II

  6807, Fall, Faculty of Agriculture, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Bioresource and Environmental Informatics I

  6805, Spring, Faculty of Agriculture, 2

Administration

Faculty management (title, position)

• From 01 Apr. 2024, To 31 Mar. 2025

  連携推進WG委員