Basic Information

Affiliated programs (koza)

• Graduate School of Science, 化学専攻 生体分子動態化学, 教授
• Graduate School of Medicine, 医科学専攻 細胞膜生物学, 教授
• Graduate School of Medicine, 医学専攻 細胞膜生物学, 教授

Academic Degree

• 理学修士(京都大学)
• 理学博士(京都大学)

Language of Instruction

• English

ID,URL

• ORCID ID

  0000-0003-4483-5408

• J-Global ID

  201401097662705880

researchmap URL

• https://researchmap.jp/yoshinoriakiyama

Research

Research Topics, Overview of the research

• Research Topics

  Biogenesis and quality control of E. coli cell surface proteins

• Overview of the research

  Our research projects are concerned with post-translational events in the expression of genetic information. Specifically, the processes of protein translation, protein translocation across and integration into the membrane, membrane protein proteolysis/assembly and extracytoplasmic stress responses are investigated by combined molecular genetic, biochemical biophysical and structural approaches.

Research Areas

• Life sciences, Molecular biology

Papers

• Cryo-EM structure of the bacterial intramembrane metalloprotease RseP in the substrate-bound state.

  Kikuko Asahi; Mika Hirose; Rie Aruga; Yosuke Shimizu; Michiko Tajiri; Tsubasa Tanaka; Yuriko Adachi; Yukari Tanaka; Mika K Kaneko; Yukinari Kato; Satoko Akashi; Yoshinori Akiyama; Yohei Hizukuri; Takayuki Kato; Terukazu Nogi

  Science advances, 28 Feb. 2025, Peer-reviewed

  
• Translation arrest cancellation of VemP, a secretion monitor in Vibrio, is regulated by multiple cis and trans factors, including SecY.

  Yuki Ikeda; Ryoji Miyazaki; Tomoya Tsukazaki; Yoshinori Akiyama; Hiroyuki Mori

  The Journal of biological chemistry, Oct. 2024, Peer-reviewed

  Access this paper :

  
• Cleavage cascade of the sigma regulator FecR orchestrates TonB-dependent signal transduction

  Tatsuhiko Yokoyama; Ryoji Miyazaki; Takehiro Suzuki; Naoshi Dohmae; Hiroki Nagai; Tomoya Tsukazaki; Tomoko Kubori; Yoshinori Akiyama

  Proceedings of the National Academy of Sciences of the United States of America, 08 Jan. 2025, Peer-reviewed, Corresponding author

  
• The origin of esterase activity of Parkinson's disease causative factor DJ-1 implied by evolutionary trace analysis of its prokaryotic homolog HchA

  Aiko Watanabe; Fumika Koyano; Kenichiro Imai; Yohei Hizukuri; Shizuka Ogiwara; Tomoya Ito; Jun Miyamoto; Chihiro Shibuya; Mayumi Kimura; Kazuya Toriumi; Chie Motono; Makoto Arai; Keiji Tanaka; Yoshinori Akiyama; Koji Yamano; Noriyuki Matsuda

  Journal of Biological Chemistry, Jun. 2024, Peer-reviewed

  
• S2P intramembrane protease RseP degrades small membrane proteins and suppresses the cytotoxicity of intrinsic toxin HokB

  Tatsuhiko Yokoyama; Yutaro Yamagata; Saisei Honna; Shinya Mizuno; Shizuka Katagiri; Rika Oi; Terukazu Nogi; Yohei Hizukuri; Yoshinori Akiyama

  mBio, 06 Jul. 2023, Peer-reviewed, Last author, Corresponding author

  Access this paper :

  
• Analyzing protein intermediate interactions in living E. coli cells using site-specific photo-crosslinking combined with chemical crosslinking.

  Ryoji Miyazaki; Yoshinori Akiyama

  STAR protocols, 17 Mar. 2023, Peer-reviewed, Invited, Corresponding author

  Access this paper :

  
• Inner membrane YfgM-PpiD heterodimer acts as a functional unit that associates with the SecY/E/G translocon and promotes protein translocation.

  Ryoji Miyazaki; Mengting Ai; Natusko Tanaka; Takehiro Suzuki; Naoshi Dhomae; Tomoya Tsukazaki; Yoshinori Akiyama; Hiroyuki Mori

  The Journal of biological chemistry, 06 Oct. 2022, Peer-reviewed

  Access this paper :

  
• A Photo-Crosslinking Approach to Monitoring the Assembly of an LptD Intermediate with LptE in a Living Cell.

  Ryoji Miyazaki; Hiroyuki Mori; Yoshinori Akiyama

  Methods in molecular biology (Clifton, N.J.), 2022, Peer-reviewed, Invited, Corresponding author

  
• Mechanistic insights into intramembrane proteolysis by E. coli site-2 protease homolog RseP.

  Yuki Imaizumi; Kazunori Takanuki; Takuya Miyake; Mizuki Takemoto; Kunio Hirata; Mika Hirose; Rika Oi; Tatsuya Kobayashi; Kenichi Miyoshi; Rie Aruga; Tatsuhiko Yokoyama; Shizuka Katagiri; Hiroaki Matsuura; Kenji Iwasaki; Takayuki Kato; Mika K Kaneko; Yukinari Kato; Michiko Tajiri; Satoko Akashi; Osamu Nureki; Yohei Hizukuri; Yoshinori Akiyama; Terukazu Nogi

  Science advances, 26 Aug. 2022, Peer-reviewed, Corresponding author

  Access this paper :

  
• Cryo-EM structure of the entire FtsH-HflKC AAA protease complex.

  Zhu Qiao; Tatsuhiko Yokoyama; Xin-Fu Yan; Ing Tsyr Beh; Jian Shi; Sandip Basak; Yoshinori Akiyama; Yong-Gui Gao

  Cell reports, 31 May 2022, Peer-reviewed, Corresponding author

  Access this paper :

  
• Edge-strand of BepA interacts with immature LptD on the β-barrel assembly machine to direct it to on- and off-pathways.

  Ryoji Miyazaki; Tetsuro Watanabe; Kohei Yoshitani; Yoshinori Akiyama

  eLife, 31 Aug. 2021, Peer-reviewed, Corresponding author

  Access this paper :

  
• Moving toward generalizable NZ-1 labeling for 3D structure determination with optimized epitope-tag insertion.

  Risako Tamura-Sakaguchi; Rie Aruga; Mika Hirose; Toru Ekimoto; Takuya Miyake; Yohei Hizukuri; Rika Oi; Mika K Kaneko; Yukinari Kato; Yoshinori Akiyama; Mitsunori Ikeguchi; Kenji Iwasaki; Terukazu Nogi

  Acta crystallographica. Section D, Structural biology, 01 May 2021, Peer-reviewed

  
• The Escherichia coli S2P intramembrane protease RseP regulates ferric citrate uptake by cleaving the sigma factor regulator FecR.

  Tatsuhiko Yokoyama; Tomoya Niinae; Kazuya Tsumagari; Koshi Imami; Yasushi Ishihama; Yohei Hizukuri; Yoshinori Akiyama

  The Journal of biological chemistry, 15 Apr. 2021, Peer-reviewed, Corresponding author

  Access this paper :

  
• Fine interaction profiling of VemP and mechanisms responsible for its translocation-coupled arrest-cancelation.

  Ryoji Miyazaki; Yoshinori Akiyama; Hiroyuki Mori

  eLife, 15 Dec. 2020, Peer-reviewed

  
• Reversible autoinhibitory regulation of Escherichia coli metallopeptidase BepA for selective β-barrel protein degradation.

  Yasushi Daimon; Shin-Ichiro Narita; Ryoji Miyazaki; Yohei Hizukuri; Hiroyuki Mori; Yoshiki Tanaka; Tomoya Tsukazaki; Yoshinori Akiyama

  Proceedings of the National Academy of Sciences of the United States of America, 10 Nov. 2020, Peer-reviewed, Corresponding author

  
• A photo-cross-linking approach to monitor protein dynamics in living cells.

  Ryoji Miyazaki; Yoshinori Akiyama; Hiroyuki Mori

  Biochimica et biophysica acta. General subjects, Feb. 2020, Peer-reviewed

  
• Involvement of a Membrane-Bound Amphiphilic Helix in Substrate Discrimination and Binding by an Escherichia coli S2P Peptidase RseP.

  Takuya Miyake; Yohei Hizukuri; Yoshinori Akiyama

  Frontiers in microbiology, 2020, Peer-reviewed, Corresponding author

  
• Identification of an internal cavity in the PhoQ sensor domain for PhoQ activity and SafA-mediated control.

  Yoshitani K; Ishii E; Taniguchi K; Sugimoto H; Shiro Y; Akiyama Y; Kato A; Utsumi R; Eguchi Y

  Bioscience, biotechnology, and biochemistry, Apr. 2019, Peer-reviewed

  
• An in vivo protease activity assay for investigating the functions of the Escherichia coli membrane protease HtpX.

  Yoshitani K; Hizukuri Y; Akiyama Y

  FEBS letters, Apr. 2019, Peer-reviewed, Corresponding author

  
• Structural Basis for the Function of the β-Barrel Assembly-Enhancing Protease BepA.

  Shahrizal M; Daimon Y; Tanaka Y; Hayashi Y; Nakayama S; Iwaki S; Narita SI; Kamikubo H; Akiyama Y; Tsukazaki T

  Journal of molecular biology, Feb. 2019, Peer-reviewed, Corresponding author

  
• Heat shock transcription factor σ³² defective in membrane transport can be suppressed by transposon insertion into genes encoding a restriction enzyme subunit or a putative autotransporter in Escherichia coli.

  Yura T; Miyazaki R; Fujiwara K; Ito K; Chiba S; Mori H; Akiyama Y

  Genes & genetic systems, Jan. 2019, Peer-reviewed, Corresponding author

  
• Identification and characterization of a translation arrest motif in VemP by systematic mutational analysis

  Hiroyuki Mori; Sohei Sakashita; Jun Ito; Eiji Ishii; Yoshinori Akiyama

  Journal of Biological Chemistry, 23 Feb. 2018, Peer-reviewed

  
• A photo-cross-linking approach to monitor folding and assembly of newly synthesized proteins in a living cell

  Ryoji Miyazaki; Naomi Myougo; Hiroyuki Mori; Yoshinori Akiyama

  Journal of Biological Chemistry, 12 Jan. 2018, Peer-reviewed, Corresponding author

  
• The TPR domain of BepA is required for productive interaction with substrate proteins and the -barrel assembly machinery complex

  Yasushi Daimon; Chigusa Iwama-Masui; Yoshiki Tanaka; Takuya Shiota; Takehiro Suzuki; Ryoji Miyazaki; Hiroto Sakurada; Trevor Lithgow; Naoshi Dohmae; Hiroyuki Mori; Tomoya Tsukazaki; Shin-ichiro Narita; Yoshinori Akiyama

  MOLECULAR MICROBIOLOGY, Dec. 2017, Peer-reviewed, Corresponding author

  
• Involvement of a conserved GFG motif region in substrate binding by RseP, an Escherichia coli S2P protease

  Koichiro Akiyama; Yohei Hizukuri; Yoshinori Akiyama

  MOLECULAR MICROBIOLOGY, Jun. 2017, Peer-reviewed, Corresponding author

  
• Biochemical Characterization of Function and Structure of RseP, an Escherichia coli S2P Protease

  Y. Hizukuri; K. Akiyama; Y. Akiyama

  ENZYMOLOGY AT THE MEMBRANE INTERFACE: INTRAMEMBRANE PROTEASES, 2017, Peer-reviewed, Corresponding author

  
• A Novel SRP Recognition Sequence in the Homeostatic Control Region of Heat Shock Transcription Factor sigma(32)

  Ryoji Miyazaki; Takashi Yura; Takehiro Suzuki; Naoshi Dohmae; Hiroyuki Mori; Yoshinori Akiyama

  SCIENTIFIC REPORTS, Apr. 2016, Peer-reviewed, Corresponding author

  Access this paper :

  
• Erratum: Nascent chain-monitored remodeling of the Sec machinery for salinity adaptation of marine bacteria (Proceedings of the National Academy of Sciences of the United States of America (2015) 112:51 (E7157))

  Eiji Ishii; Shinobu Chiba; Narimasa Hashimoto; Seiji Kojima; Michio Homma; Koreaki Ito; Yoshinori Akiyama; Hiroyuki Mori

  Proceedings of the National Academy of Sciences of the United States of America, 22 Dec. 2015

  
• 新生鎖が奏でる細胞機能制御 新生鎖の翻訳停止を介したSec膜透過装置のリモデリングによる海洋性細菌の環境適応

  森 博幸; 石井 英治; 千葉 志信; 橋本 成祐; 小嶋 誠司; 本間 道夫; 伊藤 維昭; 秋山 芳展

  日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, Dec. 2015

  
• Nascent chain-monitored remodeling of the Sec machinery for salinity adaptation of marine bacteria

  Eiji Ishii; Shinobu Chiba; Narimasa Hashimoto; Seiji Kojima; Michio Homma; Koreaki Ito; Yoshinori Akiyama; Hiroyuki Mori

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Oct. 2015, Peer-reviewed

  
• Roles of the membrane-reentrant beta-hairpin-like loop of RseP protease in selective substrate cleavage

  Koichiro Akiyama; Shinya Mizuno; Yohei Hizukuri; Hiroyuki Mori; Terukazu Nogi; Yoshinori Akiyama

  ELIFE, Oct. 2015, Peer-reviewed, Corresponding author

  Access this paper :

  
• Activation of Toxin-Antitoxin System Toxins Suppresses Lethality Caused by the Loss of sigma(E) in Escherichia coli

  Yasushi Daimon; Shin-ichiro Narita; Yoshinori Akiyama

  JOURNAL OF BACTERIOLOGY, Jul. 2015, Peer-reviewed, Corresponding author

  
• Novel functions of a bacterial extracytoplasmic stress responsev

  Yoshinori Akiyama

  IFO Research Communications, Dec. 2014, Peer-reviewed, Invited

  
• Expression and purification of integral membrane metallopeptidase HtpX

  Joan L. Arolas; Raquel Garcia-Castellanos; Theodoros Goulas; Yoshinori Akiyama; F. Xavier Gomis-Rueth

  PROTEIN EXPRESSION AND PURIFICATION, Jul. 2014, Peer-reviewed

  
• A Structure-Based Model of Substrate Discrimination by a Noncanonical PDZ Tandem in the Intramembrane-Cleaving Protease RseP

  Yohei Hizukuri; Takashi Oda; Sanae Tabata; Keiko Tamura-Kawakami; Rika Oi; Mamoru Sato; Junichi Takagi; Yoshinori Akiyama; Terukazu Nogi

  STRUCTURE, Feb. 2014, Peer-reviewed

  
• Heat Shock Transcription Factor sigma(32) Co-opts the Signal Recognition Particle to Regulate Protein Homeostasis in E. coli

  Bentley Lim; Ryoji Miyazaki; Saskia Neher; Deborah A. Siegele; Koreaki Ito; Peter Walter; Yoshinori Akiyama; Takashi Yura; Carol A. Gross

  PLOS BIOLOGY, Dec. 2013, Peer-reviewed

  
• Biochemical and structural insights into intramembrane metalloprotease mechanisms.

  Kroos L; Akiyama Y

  Biochimica et biophysica acta, Dec. 2013, Peer-reviewed

  
• Heat Shock Transcription Factor σ32 Co-opts the Signal Recognition Particle to Regulate Protein Homeostasis in E. coli

  Bentley Lim; Ryoji Miyazaki; Saskia Neher; Deborah A. Siegele; Koreaki Ito; Peter Walter; Yoshinori Akiyama; Takashi Yura; Carol A. Gross

  PLoS Biology, Dec. 2013, Peer-reviewed

  
• Protease homolog BepA (YfgC) promotes assembly and degradation of beta-barrel membrane proteins in Escherichia coli

  Shin-ichiro Narita; Chigusa Masui; Takehiro Suzuki; Naoshi Dohmae; Yoshinori Akiyama

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Sep. 2013, Peer-reviewed

  Access this paper :

  
• Structure and mechanism of rhomboid protease

  Ya Ha; Yoshinori Akiyama; Yi Xue

  Journal of Biological Chemistry, 31 May 2013, Peer-reviewed

  
• FtsH Protease

  Teru Ogura; Takashi Okuno; Ryoji Suno; Yoshinori Akiyama

  Handbook of Proteolytic Enzymes, 2013, Peer-reviewed

  
• HtpX Peptidase

  Yoshinori Akiyama; Koreaki Ito

  Handbook of Proteolytic Enzymes, 2013, Peer-reviewed

  
• RseP Peptidase

  Yohei Hizukuri; Koreaki Ito; Yoshinori Akiyama

  Handbook of Proteolytic Enzymes, 2013, Peer-reviewed

  
• 3P032 Substrate discrimination mechanism by a PDZ tandem in the intramembrane protease RseP that regulates extracytoplasmic stress response(01A. Protein: Structure,Poster)

  Hizukuri Yohei; Oda Takashi; Tabata Sanae; Tamura-Kawakami Keiko; Sato Mamoru; Takagi Junichi; Nogi Terukazu; Akiyama Yoshinori

  Seibutsu Butsuri, 2013

  
• PDZ domains of RseP are not essential for sequential cleavage of RseA or stress-induced sE activation in vivo

  Yohei Hizukuri; Yoshinori Akiyama

  MOLECULAR MICROBIOLOGY, Dec. 2012, Peer-reviewed

  
• Conformational transition of the lid helix covering the protease active site is essential for the ATP-dependent protease activity of FtsH

  Ryoji Suno; Masakazu Shimoyama; Akiko Abe; Tatsuro Shimamura; Natsuka Shimodate; Yo-hei Watanabe; Yoshinori Akiyama; Masasuke Yoshida

  FEBS LETTERS, Sep. 2012, Peer-reviewed

  
• Conformational Change in Rhomboid Protease GlpG Induced by Inhibitor Binding to Its S ' Subsites

  Yi Xue; Somenath Chowdhury; Xuying Liu; Yoshinori Akiyama; Jonathan Ellman; Ya Ha

  BIOCHEMISTRY, May 2012, Peer-reviewed

  
• 3PT122 Analysis of a regulatory mechanism of the proteolytic function of RseP by the PDZ domains in extracytoplasmic stress response(The 50th Annual Meeting of the Biophysical Society of Japan)

  Hizukuri Yohei; Akiyama Yoshinori

  Seibutsu Butsuri, 2012

  
• Nascentome Analysis Uncovers Futile Protein Synthesis in Escherichia coli

  Koreaki Ito; Yuhei Chadani; Kenta Nakamori; Shinobu Chiba; Yoshinori Akiyama; Tatsuhiko Abo

  PLOS ONE, Dec. 2011, Peer-reviewed

  
• Nascentome Analysis Uncovers Futile Protein Synthesis in Escherichia coli

  Koreaki Ito; Yuhei Chadani; Kenta Nakamori; Shinobu Chiba; Yoshinori Akiyama; Tatsuhiko Abo

  PLOS ONE, Dec. 2011, Peer-reviewed

  
• Post-liberation cleavage of signal peptides is catalyzed by the site-2 protease (S2P) in bacteria

  Akira Saito; Yohei Hizukuri; Ei-ichi Matsuo; Shinobu Chiba; Hiroyuki Mori; Osamu Nishimura; Koreaki Ito; Yoshinori Akiyama

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Aug. 2011, Peer-reviewed

  
• Recruitment of a species-specific translational arrest module to monitor different cellular processes

  Shinobu Chiba; Takashi Kanamori; Takuya Ueda; Yoshinori Akiyama; Kit Pogliano; Koreaki Ito

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Apr. 2011, Peer-reviewed

  
• Quality control of cytoplasmic membrane proteins in escherichia coli

  Yoshinori Akiyama

  Journal of Biochemistry, Oct. 2009, Peer-reviewed

  
• Quality Control of Cytoplasmic Membrane Proteins in Escherichia coli

  Yoshinori Akiyama

  JOURNAL OF BIOCHEMISTRY, Oct. 2009, Peer-reviewed

  
• A Pair of Circularly Permutated PDZ Domains Control RseP, the S2P Family Intramembrane Protease of Escherichia coli

  Kenji Inaba; Mamoru Suzuki; Ken-ichi Maegawa; Shuji Akiyama; Koreaki Ito; Yoshinori Akiyama

  JOURNAL OF BIOLOGICAL CHEMISTRY, Dec. 2008, Peer-reviewed

  
• Substrate recognition and binding by RseP, an Escherichia coli intramembrane protease

  Kayo Koide; Koreaki Ito; Yoshinori Akiyama

  JOURNAL OF BIOLOGICAL CHEMISTRY, Apr. 2008, Peer-reviewed

  
• The role of L1 loop in the mechanism of rhomboid intramembrane protease GlpG

  Yongcheng Wang; Saki Maegawa; Yoshinori Akiyama; Ya Ha

  JOURNAL OF MOLECULAR BIOLOGY, Dec. 2007, Peer-reviewed

  
• The role of L1 loop in the mechanism of rhomboid intramembrane protease GlpG

  Yongcheng Wang; Saki Maegawa; Yoshinori Akiyama; Ya Ha

  JOURNAL OF MOLECULAR BIOLOGY, Dec. 2007, Peer-reviewed

  
• Sequence features of substrates required for cleavage by GlpG, an Escherichia coli rhomboid protease

  Yoshinori Akiyama; Saki Maegawa

  MOLECULAR MICROBIOLOGY, May 2007, Peer-reviewed

  
• The intramembrane active site of GlpG, an E-coli rhomboid protease, is accessible to water and hydrolyses an extramembrane peptide bond of substrates

  Saki Maegawa; Kayo Koide; Koreaki Ito; Yoshinori Akiyama

  MOLECULAR MICROBIOLOGY, Apr. 2007, Peer-reviewed

  
• Environment of the active site region of RseP, an Escherichia coli regulated intramembrane proteolysis protease, assessed by site-directed cysteine alkylation

  Kayo Koide; Saki Maegawa; Koreaki Ito; Yoshinori Akiyama

  JOURNAL OF BIOLOGICAL CHEMISTRY, Feb. 2007, Peer-reviewed

  
• 水と油のコラボ：膜タンパク質の加水分解

  秋山芳展

  「タンパク質の一生集中マスター細胞における成熟・輸送・品質管理」p139, 2007, Peer-reviewed

  
• SecY alterations that impair membrane protein folding and generate a membrane stress

  Nobuyuki Shimohata; Shushi Nagamori; Yoshinori Akiyama; H. Ronald Kaback; Koreaki Ito

  JOURNAL OF CELL BIOLOGY, Jan. 2007, Peer-reviewed

  
• The Escherichia coli plasma membrane contains two PHB (prohibitin homology) domain protein complexes of opposite orientations

  S Chiba; K Ito; Y Akiyama

  MOLECULAR MICROBIOLOGY, Apr. 2006, Peer-reviewed

  
• Proteolytic action of GlpG, a rhomboid protease in the Escherichia coli cytoplasmic membrane

  S Maegawa; K Ito; Y Akiyama

  BIOCHEMISTRY, Oct. 2005, Peer-reviewed

  
• Proteolytic activity of HtpX, a membrane-bound and stress-controlled protease from Escherichia coli

  M Sakoh; K Ito; Y Akiyama

  JOURNAL OF BIOLOGICAL CHEMISTRY, Sep. 2005, Peer-reviewed

  
• Regulation and route-selection in SecA-SecYEG protein translocase

  Koreaki Ito; Hiromki Mori; Hitoshi Nakatogawa; Yoshinori Akiyama; Nobuyuki Sbimohata

  CELL STRUCTURE AND FUNCTION, Jun. 2005, Peer-reviewed

  
• Peculiar properties of DsbA in its export across the Escherichia coli cytoplasmic membrane

  N Shimohata; Y Akiyama; K Ito

  JOURNAL OF BACTERIOLOGY, Jun. 2005, Peer-reviewed

  
• 大腸菌の膜タンパク質分解

  酒向真智子; 秋山芳展

  実験医学 23 増刊「細胞内タンパク質 の社会学」（永田和宏、遠藤斗志也 編） 2358-2362, 2005, Peer-reviewed

  
• Cellular functions, mechanism of action, and regulation of FtsH protease

  K Ito; Y Akiyama

  ANNUAL REVIEW OF MICROBIOLOGY, 2005, Peer-reviewed

  
• RseP (YaeL), an Escherichia coli RIP protease, cleaves transmembrane sequences

  Y Akiyama; K Kanehara; K Ito

  EMBO JOURNAL, Nov. 2004, Peer-reviewed

  
• [Quality control of cell surface proteins in E. coli].

  Akiyama Y

  Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, May 2004, Peer-reviewed

  
• FtsH exists as an exceptionally large complex containing HflKC in the plasma membrane of Escherichia coli

  N Saikawa; Y Akiyama; K Ito

  JOURNAL OF STRUCTURAL BIOLOGY, Apr. 2004, Peer-reviewed

  
• YaeL proteolysis of RseA is controlled by the PDZ domain of YaeL and a Gln-rich region of RseA

  K Kanehara; K Ito; Y Akiyama

  EMBO JOURNAL, Dec. 2003, Peer-reviewed

  
• [RIP(regulated intramembrane proteolysis): from bacteria to higher organism].

  Kanehara K; Akiyama Y

  Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, Jun. 2003, Peer-reviewed

  
• Reconstitution of membrane proteolysis by FtsH

  Y Akiyama; K Ito

  JOURNAL OF BIOLOGICAL CHEMISTRY, May 2003, Peer-reviewed

  
• Fluorescence resonance energy transfer analysis of protein translocase - SecYE from Thermus thermophilus HB8 forms a constitutive oligomer in membranes

  H Mori; T Tsukazaki; R Masui; S Kuramitsu; S Yokoyama; AE Johnson; Y Kimura; Y Akiyama; K Ito

  JOURNAL OF BIOLOGICAL CHEMISTRY, Apr. 2003, Peer-reviewed

  
• A SecE mutation that modulates SecY-SecE translocase assembly, identified as a specific suppressor of SecY defects

  H Mori; Y Akiyama; K Ito

  JOURNAL OF BACTERIOLOGY, Feb. 2003, Peer-reviewed

  
• RIP(regulated intramembrane proteolysis): from bacteria to higher organism

  K. Kanehara; Y. Akiyama

  Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 2003, Peer-reviewed

  
• Membrane protein degradation,by FtsH can be initiated from either end

  S Chiba; Y Akiyama; K Ito

  JOURNAL OF BACTERIOLOGY, Sep. 2002, Peer-reviewed

  
• YaeL (EcfE) activates the sigma(E) pathway of stress response through a site-2 cleavage of anti-sigma(E), RseA

  K Kanehara; K Ito; Y Akiyama

  GENES & DEVELOPMENT, Aug. 2002, Peer-reviewed

  
• The Cpx stress response system of Escherichia coli senses plasma membrane proteins and controls HtpX, a membrane protease with a cytosolic active site

  N Shimohata; S Chiba; N Saikawa; K Ito; Y Akiyama

  GENES TO CELLS, Jul. 2002, Peer-reviewed

  
• Identification glutamic acid 479 as the gluzincin coordinator of zinc in FtsH (HflB)

  N Saikawa; K Ito; Y Akiyama

  BIOCHEMISTRY, Feb. 2002, Peer-reviewed

  
• Characterization of the yaeL gene product and its S2P-protease motifs in Escherichia coli

  K Kanehara; Y Akiyama; K Ito

  GENE, Dec. 2001, Peer-reviewed

  
• Roles of homooligomerization and membrane association in ATPase and proteolytic activities of FtsH in vitro

  Y Akiyama; K Ito

  BIOCHEMISTRY, Jun. 2001, Peer-reviewed

  
• Revisiting the lysogenization control of bacteriophage λ. Identification and characterization of a new host component, HflD

  Akio Kihara; Yoshinori Akiyama; Koreaki Ito

  Journal of Biological Chemistry, 27 Apr. 2001, Peer-reviewed

  
• Evidence for an active role of the DnaK chaperone system in the degradation of sigma(32)

  T Tatsuta; DM Joo; R Calendar; Y Akiyama; T Ogura

  FEBS LETTERS, Aug. 2000, Peer-reviewed

  
• Roles of multimerization and membrane association in the proteolytic functions of FtsH (HflB)

  Y Akiyama; K Ito

  EMBO JOURNAL, Aug. 2000, Peer-reviewed

  
• Length recognition at the N-terminal tail for the initiation of FtsH-mediated proteolysis

  S Chiba; Y Akiyama; H Mori; E Matsuo; K Ito

  EMBO REPORTS, Jul. 2000, Peer-reviewed

  
• Self-processing of FtsH and its implication for the cleavage specificity of this protease

  Y Akiyama

  BIOCHEMISTRY, Sep. 1999, Peer-reviewed

  
• Dislocation of membrane proteins in FtsH-mediated proteolysis

  A Kihara; Y Akiyama; K Ito

  EMBO JOURNAL, Jun. 1999, Peer-reviewed

  
• A class of integral membrane proteins will be overlooked by the 'proteome' study that is based on two-dimensional gel electrophoresis

  K Ito; E Matsuo; Y Akiyama

  MOLECULAR MICROBIOLOGY, Mar. 1999, Peer-reviewed

  
• Roles of the periplasmic domain of Escherichia coli FtsH (HflB) in protein interactions and activity modulation

  Y Akiyama; A Kihara; H Mori; T Ogura; K Ito

  JOURNAL OF BIOLOGICAL CHEMISTRY, Aug. 1998, Peer-reviewed

  
• Different pathways for protein degradation by the FtsH/HflKC membrane-embedded protease complex: An implication from the interference by a mutant form of a new substrate protein, YccA

  Akio Kihara; Yoshinori Akiyama; Koreaki Ito

  Journal of Molecular Biology, 29 May 1998, Peer-reviewed

  
• Polypeptide binding of Escherichia coli FtsH (HflB)

  Y Akiyama; M Ehrmann; A Kihara; K Ito

  MOLECULAR MICROBIOLOGY, May 1998, Peer-reviewed

  
• Differential in vivo roles played by DsbA and DsbC in the formation of protein disulfide bonds

  M Sone; Y Akiyama; K Ito

  JOURNAL OF BIOLOGICAL CHEMISTRY, Apr. 1997, Peer-reviewed

  
• Control of cellular functions by ATP-dependent proteases in prokaryotes

  T. Ogura; Y. Akiyama

  Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 1997, Peer-reviewed

  
• The SecY complex and the FtsH complex in the E-coli plasma membrane

  K Ito; Y Akiyama; A Kihara; E Matsuo; T Homma; Y Shirai; T Yoshihisa; T Taura; T Shimoike; T Baba; M Sone; G Matsumoto

  MEMBRANE PROTEINS - STRUCTURE, FUNCTION AND EXPRESSION CONTROL: INTERNATIONAL SYMPOSIUM, 1997, Peer-reviewed

  
• Subunit a of proton ATPase F0 sector is a substrate of the FtsH protease in Escherichia coli

  Yoshinori Akiyama; Akio Kihara; Koreaki Ito

  FEBS Letters, 09 Dec. 1996, Peer-reviewed

  
• FtsH (HflB) is an ATP-dependent protease selectively acting on SecY and some other membrane proteins

  Y Akiyama; A Kihara; H Tokuda; K Ito

  JOURNAL OF BIOLOGICAL CHEMISTRY, Dec. 1996, Peer-reviewed

  
• A protease complex in the Escherichia coli plasma membrane: HflKC (HflA) forms a complex with FtsH (HflB), regulating its proteolytic activity against SecY

  Akio Kihara; Yoshinori Akiyama; Koreaki Ito

  EMBO Journal, 15 Nov. 1996, Peer-reviewed

  
• The tolZ gene of Escherichia coli is identified as the ftsH gene

  JN Qu; S Makino; H Adachi; Y Koyama; Y Akiyama; K Ito; T Tomoyasu; T Ogura; H Matsuzawa

  JOURNAL OF BACTERIOLOGY, Jun. 1996, Peer-reviewed

  
• Suppression of ftsH mutant phenotypes by overproduction of molecular chaperones

  Y Shirai; Y Akiyama; K Ito

  JOURNAL OF BACTERIOLOGY, Feb. 1996, Peer-reviewed

  
• A NEW ESCHERICHIA-COLI GENE, FDRA, IDENTIFIED BY SUPPRESSION ANALYSIS OF DOMINANT-NEGATIVE FTSH MUTATIONS

  Y AKIYAMA; K ITO

  MOLECULAR AND GENERAL GENETICS, Nov. 1995, Peer-reviewed

  
• FTSH, A MEMBRANE-BOUND ATPASE, FORMS A COMPLEX IN THE CYTOPLASMIC MEMBRANE OF ESCHERICHIA-COLI

  Y AKIYAMA; T YOSHIHISA; K ITO

  JOURNAL OF BIOLOGICAL CHEMISTRY, Oct. 1995, Peer-reviewed

  
• INTRACELLULAR STABILITY OF ALPHA-FRAGMENTS OF BETA-GALACTOSIDASE - EFFECTS OF AMINO-TERMINALLY FUSED POLYPEPTIDES

  T HOMMA; T YOSHIHISA; A KIHARA; Y AKIYAMA; K ITO

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Oct. 1995, Peer-reviewed

  
• REDOX STATES OF DSBA IN THE PERIPLASM OF ESCHERICHIA-COLI

  S KISHIGAMI; Y AKIYAMA; K ITO

  FEBS LETTERS, May 1995, Peer-reviewed

  
• PRODUCT OF A NEW GENE, SYD, FUNCTIONALLY INTERACTS WITH SECY WHEN OVERPRODUCED IN ESCHERICHIA-COLI

  T SHIMOIKE; T TAURA; A KIHARA; T YOSHIHISA; Y AKIYAMA; K CANNON; K ITO

  JOURNAL OF BIOLOGICAL CHEMISTRY, Mar. 1995, Peer-reviewed

  
• GENETIC-ANALYSIS OF SECY - ADDITIONAL EXPORT DEFECTIVE MUTATIONS AND FACTORS AFFECTING THEIR PHENOTYPES

  T TAURA; Y AKIYAMA; K ITO

  MOLECULAR & GENERAL GENETICS, May 1994, Peer-reviewed

  
• Involvement of FtsH in protein assembly into and through the membrane. I. Mutations that reduce retention efficiency of a cytoplasmic reporter

  Y. Akiyama; T. Ogura; K. Ito

  Journal of Biological Chemistry, 1994, Peer-reviewed

  
• DETERMINANTS OF THE QUANTITY OF THE STABLE SECY COMPLEX IN THE ESCHERICHIA-COLI CELL

  T TAURA; T BABA; Y AKIYAMA; K ITO

  JOURNAL OF BACTERIOLOGY, Dec. 1993, Peer-reviewed

  
• FOLDING AND ASSEMBLY OF BACTERIAL ALKALINE-PHOSPHATASE INVITRO AND INVIVO

  Y AKIYAMA; K ITO

  JOURNAL OF BIOLOGICAL CHEMISTRY, Apr. 1993, Peer-reviewed

  
• INVITRO CATALYSIS OF OXIDATIVE FOLDING OF DISULFIDE-BONDED PROTEINS BY THE ESCHERICHIA-COLI DSBA (PPFA) GENE-PRODUCT

  Y AKIYAMA; S KAMITANI; N KUSUKAWA; K ITO

  JOURNAL OF BIOLOGICAL CHEMISTRY, Nov. 1992, Peer-reviewed

  
• SECY VARIANTS THAT INTERFERE WITH ESCHERICHIA-COLI PROTEIN EXPORT IN THE PRESENCE OF NORMAL SECY

  T SHIMOIKE; Y AKIYAMA; T BABA; T TAURA; K ITO

  MOLECULAR MICROBIOLOGY, May 1992, Peer-reviewed

  
• IDENTIFICATION AND CHARACTERIZATION OF AN ESCHERICHIA-COLI GENE REQUIRED FOR THE FORMATION OF CORRECTLY FOLDED ALKALINE-PHOSPHATASE, A PERIPLASMIC ENZYME

  S KAMITANI; Y AKIYAMA; K ITO

  EMBO JOURNAL, Jan. 1992, Peer-reviewed

  
• INVIVO ANALYSIS OF INTEGRATION OF MEMBRANE-PROTEINS IN ESCHERICHIA-COLI

  K ITO; Y AKIYAMA

  MOLECULAR MICROBIOLOGY, Sep. 1991, Peer-reviewed

  
• Membrane components of the protein secretion machinery.

  K. Ito; Y. Akiyama

  Methods in cell biology, 1991, Peer-reviewed

  
• CHARACTERIZATION OF COLD-SENSITIVE SECY MUTANTS OF ESCHERICHIA-COLI

  T BABA; A JACQ; E BRICKMAN; J BECKWITH; T TAURA; C UEGUCHI; Y AKIYAMA; K ITO

  JOURNAL OF BACTERIOLOGY, Dec. 1990, Peer-reviewed

  
• A POSITIVELY CHARGED REGION IS A DETERMINANT OF THE ORIENTATION OF CYTOPLASMIC MEMBRANE-PROTEINS IN ESCHERICHIA-COLI

  K YAMANE; Y AKIYAMA; K ITO; S MIZUSHIMA

  JOURNAL OF BIOLOGICAL CHEMISTRY, Dec. 1990, Peer-reviewed

  
• SECY, A MULTISPANNING INTEGRAL MEMBRANE-PROTEIN, CONTAINS A POTENTIAL LEADER PEPTIDASE CLEAVAGE SITE

  Y AKIYAMA; T INADA; Y NAKAMURA; K ITO

  JOURNAL OF BACTERIOLOGY, Jun. 1990, Peer-reviewed

  
• SECY PROTEIN, A MEMBRANE-EMBEDDED SECRETION FACTOR OF ESCHERICHIA-COLI, IS CLEAVED BY THE OMPT PROTEASE INVITRO

  Y AKIYAMA; K ITO

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Mar. 1990, Peer-reviewed

  
• STRUCTURE AND FUNCTION OF ESCHERICHIA-COLI SECY PROTEIN, AN INTEGRAL MEMBRANE COMPONENT OF THE PROTEIN TRANSLOCATION SYSTEM

  K ITO; Y AKIYAMA; C UEGUCHI; T TAURA; T BABA

  PROTEIN ENGINEERING //, 1990, Peer-reviewed

  
• EFFECTS OF MUTATIONS IN HEAT-SHOCK GENES GROES AND GROEL ON PROTEIN EXPORT IN ESCHERICHIA-COLI

  N KUSUKAWA; T YURA; C UEGUCHI; Y AKIYAMA; K ITO

  EMBO JOURNAL, Nov. 1989, Peer-reviewed

  
• THE SECY-RPMJ REGION OF THE SPC RIBOSOMAL-PROTEIN OPERON IN ESCHERICHIA-COLI - STRUCTURAL ALTERATIONS AFFECTING SECY EXPRESSION

  C UEGUCHI; M WITTEKIND; M NOMURA; Y AKIYAMA; K ITO

  MOLECULAR AND GENERAL GENETICS, May 1989, Peer-reviewed

  
• TEMPERATURE-SENSITIVE SEC MUTANTS OF ESCHERICHIA-COLI - INHIBITION OF PROTEIN EXPORT AT THE PERMISSIVE TEMPERATURE

  K ITO; Y HIROTA; Y AKIYAMA

  JOURNAL OF BACTERIOLOGY, Mar. 1989, Peer-reviewed

  
• EXPORT OF ESCHERICHIA-COLI ALKALINE-PHOSPHATASE ATTACHED TO AN INTEGRAL MEMBRANE-PROTEIN, SECY

  Y AKIYAMA; K ITO

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jan. 1989, Peer-reviewed

  
• TOPOLOGY ANALYSIS OF THE SECY PROTEIN, AN INTEGRAL MEMBRANE-PROTEIN INVOLVED IN PROTEIN EXPORT IN ESCHERICHIA-COLI

  Y AKIYAMA; K ITO

  EMBO JOURNAL, Nov. 1987, Peer-reviewed

  
• OVERPRODUCTION, ISOLATION AND DETERMINATION OF THE AMINO-TERMINAL SEQUENCE OF THE SECY PROTEIN, A MEMBRANE-PROTEIN INVOLVED IN PROTEIN EXPORT IN ESCHERICHIA-COLI

  Y AKIYAMA; K ITO

  EUROPEAN JOURNAL OF BIOCHEMISTRY, Sep. 1986, Peer-reviewed

  
• DIVERSE EFFECTS OF THE MALE-LACZ HYBRID PROTEIN ON ESCHERICHIA-COLI CELL PHYSIOLOGY

  K ITO; Y AKIYAMA; T YURA; K SHIBA

  JOURNAL OF BACTERIOLOGY, Jul. 1986, Peer-reviewed

  
• INHIBITION OF F-PLASMID REPLICATION IN HTPR MUTANTS OF ESCHERICHIA-COLI DEFICIENT IN SIGMA-32 PROTEIN

  C WADA; Y AKIYAMA; K ITO; T YURA

  MOLECULAR & GENERAL GENETICS, 1986, Peer-reviewed

  
• PROTEIN BLOTTING THROUGH A DETERGENT LAYER, A SIMPLE METHOD FOR DETECTING INTEGRAL MEMBRANE-PROTEINS SEPARATED BY SDS-POLYACRYLAMIDE GEL-ELECTROPHORESIS

  K ITO; Y AKIYAMA

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, Peer-reviewed

  
• THE SECY MEMBRANE COMPONENT OF THE BACTERIAL PROTEIN EXPORT MACHINERY - ANALYSIS BY NEW ELECTROPHORETIC METHODS FOR INTEGRAL MEMBRANE-PROTEINS

  Y AKIYAMA; K ITO

  EMBO JOURNAL, 1985, Peer-reviewed

  
• X-ray crystal structural analysis of membrane bound ATP-dependent protease FtsH

  R. SUNO; Y. AKIYAMA; S. IWATA; M. YOSHIDA

  the Chemical Resources Laboratory, Tokyo Institute of Technology, 2Insitute for Virus Research, Kyoto University, department of Medicine, Kyoto University, Peer-reviewed

  

Misc.

• Analysis of SDS sensitivity in Escherichia coli strains lacking BepA and BamB

  成田新一郎; 渡邉きくの; 秋山芳展

  日本農芸化学会大会講演要旨集(Web), 2024

  
• 超好熱菌Aquifex aeolicus由来膜内切断プロテアーゼRsePの基質結合状態のクライオ電子顕微鏡構造

  旭紀久子; 廣瀬未果; 有賀理江; 清水洋祐; 田尻道子; 田中翼; 安達友里子; 田中ゆかり; 金子美華; 加藤幸成; 明石知子; 秋山芳展; 檜作洋平; 加藤貴之; 禾晃和

  日本結晶学会年会講演要旨集, 2024

  
• 細菌外膜品質管理プロテアーゼBepA可動ループ領域の機能解析

  宮崎亮次; 西建留; 木元麻衣; 大門康志; 塚崎智也; 秋山芳展

  日本分子生物学会年会プログラム・要旨集(Web), 2024

  
• グラム陰性細菌において、鉄シグナル伝達を担う一回膜貫通タンパク質FecRの膜挿入・連続的切断機構の解析

  横山 達彦; 塚崎 智也; 秋山 芳展

  日本生化学会大会プログラム・講演要旨集, Oct. 2023

  
• YhbOのオキソアルデヒド代謝から推察するパーキンソン病原因因子DJ-1の分子機能

  渡辺 藍子; 檜作 洋平; 秋山 芳展; 松田 憲之

  日本生化学会大会プログラム・講演要旨集, Oct. 2023

  
• 細菌の遺伝子発現制御の新展開 ビブリオ属細菌における翻訳アレストを介した遺伝子発現制御

  石井 英治; 松田 重輝; 飯田 哲也; 秋山 芳展; 森 博幸

  日本細菌学雑誌, Feb. 2023

  
• 細菌膜内切断プロテアーゼRsePの基質取り込み・切断時におけるダイナミックな構造変化モデル

  三宅 拓也; 今泉 友希; 高貫 一徳; 小林 達也; 横山 達彦; 大井 里香; 檜作 洋平; 禾 晃和; 秋山 芳展

  日本生化学会大会プログラム・講演要旨集, Nov. 2022

  
• バクテリアの表層変化と生存戦略 細菌パーシスター化における膜内切断プロテアーゼRsePの役割 生理学的及び構造学的アプローチ

  檜作 洋平; 横山 達彦; 三宅 拓也; 小林 達也; 今泉 友希; 高貫 一徳; 大井 里香; 禾 晃和; 秋山 芳展

  日本細菌学雑誌, Feb. 2022

  
• TAシステムを介した細菌の休眠と覚醒:生化学と一細胞解析の融和を目指したアプローチ 大腸菌膜内切断プロテアーゼRsePを介したTA systemの制御 細胞休眠・覚醒における意義

  檜作 洋平; 横山 達彦; 秋山 芳展

  日本生化学会大会プログラム・講演要旨集, Sep. 2020

  
• 細菌の外膜生合成と品質管理に関わる2機能性プロテアーゼBepAの基質認識機構

  宮崎 亮次; 吉谷 亘平; 森 博幸; 秋山 芳展

  日本生化学会大会プログラム・講演要旨集, Sep. 2020

  
• バクテリアの表層構造の構築と機能 大腸菌内膜で働く膜内切断プロテアーゼRsePの新規切断基質探索 パーシスター化制御への関与

  檜作 洋平; 横山 達彦; 秋山 芳展

  日本細菌学雑誌, Jan. 2020

  
• FRET蛍光ペプチドを用いた大腸菌S2Pファミリー膜内切断プロテアーゼのリアルタイム切断kinetics計測系の構築

  檜作 洋平; 秋山 芳展

  日本生化学会大会プログラム・講演要旨集, Sep. 2019

  
• 大腸菌膜内切断プロテアーゼRsePの新規基質切断とその生理的意義 低分子膜タンパク質HokBの切断を介したパーシスター形成制御

  横山 達彦; 檜作 洋平; 秋山 芳展

  日本生化学会大会プログラム・講演要旨集, Sep. 2019

  
• 大腸菌膜内切断プロテアーゼRsePの基質切断と構造維持に重要な膜表在・両親媒性ヘリックスの解析

  三宅 拓也; 檜作 洋平; 秋山 芳展

  日本生化学会大会プログラム・講演要旨集, Sep. 2019

  
• 改良型部位特異的in vivo光架橋法による生細胞内での翻訳途上ポリペプチド鎖の相互作用動態解析

  宮崎 亮次; 秋山 芳展; 森 博幸

  日本生化学会大会プログラム・講演要旨集, Sep. 2019

  
• 膜結合型AAA+プロテアーゼFtsH基質分解モデルの生化学的検討

  寿野良二; 下立夏香; 下山真和; 秋山芳展; 吉田賢右

  生化学, 25 Sep. 2009

  
• 蛍光相関分光で見る生体系の情報伝達(6)原核生物における蛍光相関分光法測定-原核細胞内タンパク質品質管理機構の解明に向けて-

  北村朗; 北村朗; 寿野良二; 寿野良二; 秋山芳展; 吉田腎右; 金城政孝

  蛍光相関分光で見る生体系の情報伝達6 理研シンポジウム 平成21年, 2009

  
• A novel physiological function of RseP, an E. coli intramembrane protease: Transcriptional activation of genes involved in iron uptake through the cleavage of FecR.

  横山達彦; 新苗智也; 津曲和哉; 今見考志; 石濱泰; 檜作洋平; 秋山芳展

  日本農芸化学会大会講演要旨集(Web), 2021

  
• PiXie Analysis for Monitoring Dynamic Protein Interaction and Folding in a Living Cell

  MIYAZAKI Ryoji; MORI Hiroyuki; AKIYAMA Yoshinori

  Seibutsu Butsuri, 2021

  
• 大腸菌S2PファミリープロテアーゼRsePの基質探索:鉄取り込みに関与する転写制御因子の切断とその生理的意義

  横山達彦; 新苗智也; 津曲和哉; 今見考志; 石濱泰; 檜作洋平; 秋山芳展

  日本生化学会大会(Web), 2020

  
• Promotion of mRNA degradation by the nascent polypeptide chain-dependent membrane localization in bacteria

  石井英治; 石井英治; 秋山芳展; 森博幸

  日本農芸化学会大会講演要旨集(Web), 2020

  
• バクテリアの表層構造の構築と機能 大腸菌内膜で働く膜内切断プロテアーゼRsePの新規切断基質探索 パーシスター化制御への関与

  檜作 洋平; 横山 達彦; 秋山 芳展

  日本細菌学雑誌, Jan. 2020

  
• FRET蛍光ペプチドを用いた大腸菌S2Pファミリー膜内切断プロテアーゼのリアルタイム切断kinetics計測系の構築

  檜作 洋平; 秋山 芳展

  日本生化学会大会プログラム・講演要旨集, Sep. 2019

  
• 大腸菌膜内切断プロテアーゼRsePの新規基質切断とその生理的意義 低分子膜タンパク質HokBの切断を介したパーシスター形成制御

  横山 達彦; 檜作 洋平; 秋山 芳展

  日本生化学会大会プログラム・講演要旨集, Sep. 2019

  
• 大腸菌膜内切断プロテアーゼRsePの基質切断と構造維持に重要な膜表在・両親媒性ヘリックスの解析

  三宅 拓也; 檜作 洋平; 秋山 芳展

  日本生化学会大会プログラム・講演要旨集, Sep. 2019

  
• 改良型部位特異的in vivo光架橋法による生細胞内での翻訳途上ポリペプチド鎖の相互作用動態解析

  宮崎 亮次; 秋山 芳展; 森 博幸

  日本生化学会大会プログラム・講演要旨集, Sep. 2019

  
• 大腸菌PhoQセンサードメインのSDポケットの構造と機能

  吉谷亘平; 石井英治; 吉谷亘平; 石井英治; 谷口勝英; 杉本宏; 杉本宏; 城宜嗣; 秋山芳展; 加藤明宣; 内海龍太郎; 江口陽子; 江口陽子

  日本農芸化学会大会講演要旨集(Web), 2019

  
• ビブリオ菌の新生ポリペプチド鎖を介した発現制御におけるmRNA二次構造の役割

  石井英治; 秋山芳展; 森博幸

  日本農芸化学会大会講演要旨集(Web), 2019

  
• 細菌の翻訳途上鎖による翻訳アレストを介したmRNA分解機構の解明

  石井英治; 秋山芳展; 森博幸

  日本分子生物学会年会プログラム・要旨集(Web), 2019

  
• 大腸菌表層ストレス応答 : インプットとアウトプットに働くプロテアーゼの機能 (助成研究のその後)

  秋山 芳展

  Research communications = 発酵研究所研究報告, 2019

  
• 大腸菌膜内切断プロテアーゼRsePの構造維持と機能調節に関わる新規ドメインの解析

  三宅拓也; 檜作洋平; 秋山芳展

  日本生化学会大会(Web), 2018

  
• 大腸菌内膜プロテアーゼHtpXのプロテアーゼ活性能評価系の構築

  吉谷亘平; 秋山芳展

  日本生化学会大会(Web), 2018

  
• 細菌べん毛III型分泌装置構成因子の翻訳後多段階プロセシング

  檜作洋平; 鈴木健裕; 照島功祐; 堂前直; 秋山芳展

  日本生化学会大会(Web), 2018

  
• 大腸菌のペリプラズムプロテアーゼBepAは,TPR domainを介したタンパク質間相互作用により外膜機能を維持する

  大門康志; 舛井千草; 宮崎亮次; 櫻田洋人; 田中良樹; 鈴木健裕; 堂前直; 森博幸; 塚崎智也; 成田新一郎; 秋山芳展

  日本農芸化学会大会講演要旨集(Web), 05 Mar. 2017

  
• 部位特異的in vivo光架橋法による生細胞内での新生タンパク質のフォールディング・複合体形成の動的過程の解析

  宮崎亮次; 明後尚美; 森博幸; 秋山芳展

  日本生化学会大会(Web), 2017

  
• 細菌RhomboidファミリープロテアーゼGlpGはべん毛III型分泌装置の構成因子の分解に関与する

  檜作洋平; 照島功祐; 秋山芳展

  日本生化学会大会(Web), 2017

  
• 細菌の表層ストレス応答に関わる膜内切断プロテアーゼRsePの新規機能調節領域の解析

  三宅拓也; 檜作洋平; 秋山芳展

  日本生化学会大会(Web), 2017

  
• 新生ポリペプチド鎖を介した発現制御におけるmRNA二次構造形成の重要性

  石井英治; 石井英治; 坂下宗平; 秋山芳展; 森博幸

  日本生化学会大会(Web), 2017

  
• 超高親和性タグを内部に挿入した複数回膜貫通型タンパク質の精製法の検討

  高貫一徳; 大井里香; 有森貴夫; 三宅拓也; 檜作洋平; 秋山芳展; 加藤幸成; 高木淳一; 禾晃和

  日本生化学会大会(Web), 2017

  
• 部位特異的in vivo光架橋法を用いた生細胞内の動的タンパク質間相互作用解析

  森博幸; 宮崎亮次; 町田裕紀子; 三登一八; 何あゆみ; 秋山光市郎; 大門康志; 舛井千草; 塚崎智也; 堂前直; 成田新一郎; 秋山芳展

  日本蛋白質科学会年会プログラム・要旨集, 19 May 2016

  
• 細菌RhomboidプロテアーゼGlpGの生理的切断基質の探索及びべん毛III型分泌装置との関係

  檜作洋平; 照島功祐; 秋山芳展

  日本生化学会大会(Web), 2016

  
• ビブリオ属細菌VemPの翻訳アレストを介したV.SecD2発現制御におけるvemP‐V.secD2遺伝子間領域で形成される巨大な二次構造の重要性

  石井英治; 坂下宗平; 秋山芳展; 森博幸

  日本分子生物学会年会プログラム・要旨集(Web), 2016

  
• Nascent chain-monitored remodeling of the Sec machinery for salinity adaptation of marine bacteria (vol 112, pg E5513, 2015)

  Eiji Ishii; Shinobu Chiba; Narimasa Hashimoto; Seiji Kojima; Michio Homma; Koreaki Ito; Yoshinori Akiyama; Hiroyuki Mori

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Dec. 2015

  
• σ^Eの欠失による大腸菌の致死性はTAシステムトキシンの活性化により抑制される

  大門康志; 成田新一郎; 秋山芳展

  日本農芸化学会大会講演要旨集(Web), 05 Mar. 2015

  
• 熱ショック転写因子σ³²とシグナル認識粒子の相互作用

  宮崎亮次; 由良隆; 鈴木健裕; 堂前直; 森博幸; 秋山芳展

  日本生化学会大会(Web), 2015

  
• 細菌表層ストレス応答を制御するS2Pファミリー膜内切断プロテアーゼの細胞内イメージング解析

  檜作洋平; 秋山芳展

  日本生化学会大会(Web), 2015

  
• ビブリオ属細菌の低食塩環境への適応:アレストペプチドVemPによるSecDF発現制御の役割

  石井英治; 千葉志信; 橋本成祐; 小嶋誠司; 本間道夫; 伊藤維昭; 秋山芳展; 森博幸

  日本生化学会大会(Web), 2015

  
• S2Pファミリー膜内切断プロテアーゼRsePの膜内挿入ループ領域を介した基質選別

  秋山光市郎; 水野慎也; 檜作洋平; 森博幸; 禾晃和; 秋山芳展

  日本生化学会大会(Web), 2015

  
• Novel functions of a bacterial extracytoplasmic stress response

  秋山芳展; 檜作洋平; 禾晃和; 小田隆士; 佐藤衛; 高木淳; 田畑早苗; 川上(田村)恵; 成田新一郎; 舛井千草; 堂前直; 鈴木健裕

  Inst Ferment Osaka Res Commun, 20 Dec. 2014

  
• 大腸菌膜内切断プロテアーゼRsePの保存された膜内挿入ループ領域の機能解析

  AKIYAMA KOICHIRO; MIZUNO SHIN'YA; HIZUKURI YOHEI; NOGI TERUKAZU; MORI HIROYUKI; AKIYAMA YOSHINORI

  日本蛋白質科学会年会プログラム・要旨集, 26 May 2014

  
• 大腸菌膜内切断プロテアーゼRsePの保存された膜内挿入ループ領域の機能解析

  AKIYAMA KOICHIRO; MIZUNO SHIN'YA; HIZUKURI YOHEI; NOGI TERUKAZU; MORI HIROYUKI; AKIYAMA YOSHINORI

  日本蛋白質科学会年会プログラム・要旨集, 26 May 2014

  
• 細菌表層ストレス応答制御に関わる膜内切断プロテアーゼRsePのタンデムPDZドメインによる切断基質選別機構の解析

  HIZUKURI YOHEI; ODA TAKASHI; TABATA SANAE; KAWAKAMI(TAMURA) KEIKO; OI RIKA; SATO MAMORU; TAKAGI JUN'ICHI; NOGI TERUKAZU; AKIYAMA YOSHINORI

  日本生化学会大会(Web), 2014

  
• 大腸菌プロテアーゼBepAは外膜タンパク質の組立と分解を促進する

  NARITA SHIN'ICHIRO; MASUI CHIGUSA; SUZUKI TAKEHIRO; DOMAE NAOSHI; AKIYAMA YOSHINORI

  日本農芸化学会東北支部大会プログラム・講演要旨集, 26 Oct. 2013

  
• 部位特異的 in vivo 光架橋法によるタンパク質膜透過促進因子SecDF の基質結合部位の探索

  三登 一八; 町田 裕紀子; 塚崎 智也; 伊藤 維昭; 秋山 芳展; 森 博幸

  第１０回 ２１世紀大腸菌研究会、伊豆, Jun. 2013

  
• AAA+プロテアーゼFtsHのATP依存的な構造変化にともなう基質ポリペプチド鎖分解メカニズム

  SUNO RYOJI; SHIMAMURA TATSURO; ABE AKIKO; HINO TOMOYA; WATANABE YOHEI; SHIMODATE NATSUKA; AKIYAMA YOSHINORI; IWATA SO; YOSHIDA MASASUKE

  日本蛋白質科学会年会プログラム・要旨集, 31 May 2013

  
• 大腸菌βバレル型膜タンパク質の生合成に関わるBepA(YfgC)の機能解析

  NARITA SHIN'ICHIRO; SUZUKI TAKEHIRO; DOMAE NAOSHI; AKIYAMA YOSHINORI

  日本農芸化学会大会講演要旨集(Web), 05 Mar. 2013

  
• 大腸菌膜内切断プロテアーゼRsePの基質認識におけるPDZドメインの役割

  HIZUKURI YOHEI; NOGI TERUKAZU; ODA TAKASHI; TABATA SANAE; KAWAKAMI(TAMURA) KEIKO; SATO MAMORU; TAKAGI JUN'ICHI; AKIYAMA YOSHINORI

  日本生化学会大会(Web), 2013

  
• 大腸菌熱ショック転写因子σ³²の膜へのtargetingを介した機能制御機構

  MIYAZAKI RYOJI; YURA TAKASHI; MORI HIROYUKI; AKIYAMA YOSHINORI

  日本生化学会大会(Web), 2013

  
• 立体構造解析に基づく大腸菌膜内切断プロテアーゼRsePのタンデムPDZドメインによる切断基質選別機構モデル

  HIZUKURI YOHEI; ODA TAKASHI; TABATA SANAE; KAWAKAMI(TAMURA) KEIKO; SATO MAMORU; TAKAGI JUN'ICHI; NOGI TERUKAZU; AKIYAMA YOSHINORI

  日本分子生物学会年会プログラム・要旨集(Web), 2013

  
• 1SDA-01 Structure and function of SecDF, a membrane integrated protein translocation enhancing factor(1SDA Prof Berg's featured lecture and dancing harmonized motility machineries,Symposium,The 51th Annual Meeting of the Biophysical Society of Japan)

  Mori Hiroyuki; Mito Kazuya; Machida Yukiko; Tsukazaki Tomoya; Ito Koreaki; Akiyama Yoshinori

  Seibutsu Butsuri, 2013

  
• 膜内切断プロテアーゼRsePの基質取り込み制御におけるPDZタンデムの役割

  NOGI TERUKAZU; HIZUKURI YOHEI; TABATA SANAE; KAWAKAMI(TAMURA) KEIKO; ODA TAKASHI; SATO MAMORU; TAKAGI JUN'ICHI; AKIYAMA YOSHINORI

  日本蛋白質科学会年会プログラム・要旨集, 31 May 2012

  
• 大腸菌細胞表層の品質管理に関わるプロテアーゼホモログYfgCの機能解析

  NARITA SHIN'ICHIRO; AKIYAMA YOSHINORI

  日本細菌学雑誌, 25 Feb. 2012

  
• 大腸菌表層ストレス応答に関与する膜内切断プロテアーゼRseP のPDZ ドメイ ンによる新たな機能制御メカニズム

  檜作洋平; 禾 晃和; 田畑早苗; 川上-田村恵子; 小田 隆; 佐藤 衛; 高木淳一; 秋山芳展

  第6 回細菌学若手コロッセウム、八王子、2 012 年8月8日-10日, 2012, Peer-reviewed

  
• 大腸菌熱ショック応答に関わる転写因子σ32 の膜へのtargeting を介した機能調節機構の解析

  宮崎亮次; 由良 隆; 森 博幸; 秋山芳展

  2012年度国立遺伝学研究所研 究会「代謝、増殖、分裂研究会」、三島、2 0 12 年12 月8 日-9 日, 2012, Peer-reviewed

  
• 大腸菌RIPプロテアーゼRsePのタンデムPDZドメインによる新たな機能制御メカニズム

  HIZUKURI YOHEI; NOGI TERUKAZU; TABATA SANAE; KAWAKAMI(TAMURA) KEIKO; ODA TAKASHI; SATO MAMORU; TAKAGI JUN'ICHI; AKIYAMA YOSHINORI

  日本生化学会大会(Web), 2012

  
• 膜結合型AAA+プロテアーゼFtsHのATP結合による基質ポリペプチド分解メカニズム

  SUNO RYOJI; SHIMAMURA TATSURO; ABE AKIKO; HINO TOMOYA; SHIMODATE NATSUKA; WATANABE YOHEI; AKIYAMA YOSHINORI; IWATA SO; YOSHIDA MASASUKE

  日本生化学会大会(Web), 2012

  
• ビブリオ菌のタンパク質分泌マシナリー:SecDFパラログの発現制御機構

  MORI HIROYUKI; HASHIMOTO NARIMASA; KOJIMA SEIJI; HONMA MICHIO; AKIYAMA YOSHINORI

  日本生化学会大会(Web), 2012

  
• Analysis of a regulatory mechanism of the proteolytic function of RseP by the PDZ domains in extracytoplasmic stress response.

  檜作洋平; 秋山芳展

  日本生物物理学会第50 回年会、名古屋、2012 年9 月 22日-24日, 2012, Peer-reviewed

  
• 大腸菌RIPプロテアーゼRseP のタンデムPDZ ドメインによる新たな機能制御メカニズム

  檜作洋平; 禾 晃和; 田畑早苗; 川上-田村恵子; 小田 隆; 佐藤 衛; 高木淳一; 秋山芳展

  第85 回日本生化学会大会、福岡、2012年12月14 日-16 日, 2012, Peer-reviewed

  
• ビブリオ菌SecDF パラログの生理機能の解明に向けて

  橋本成祐; 森博幸; 秋山芳展; 本間道夫; 小嶋誠司

  第9 回21世紀大腸菌研究会、長浜、2012年6月21日- 22日, 2012, Peer-reviewed

  
• 大腸菌におけるGE存性表層ストレス応答とtoxin-antitoxin system の関わり

  大門康志; 成田新一郎; 秋山芳展

  第9 回21世紀大腸菌研究会、長浜、2012年6月21 日-22 日, 2012, Peer-reviewed

  
• Nascentome:合成途上鎖の解析

  伊藤維昭; 茶谷悠平; 中森健太; 千葉志信; 秋山芳展; 阿保達彦

  第9回21世紀大腸菌研究会、長浜、2012年6月21日-22 日, 2012, Peer-reviewed

  
• 翻訳途上鎖MifM に よるタンパク質膜組込のモニタリング

  千葉志信; 金森 崇; 上田卓也; 秋山芳展; Kit Pogliano; 伊藤維昭

  第1 回RIBOSOME MEETING 、東広島、2012 年3 月15 日-16 日, 2012, Peer-reviewed

  
• Membrane targeting of heat shock transcription factor sigma 32: An essential step for feedback control

  Takashi Yura; Bentley Lim; Carol Gross; Hiroyuki Mori; Koreaki Ito; Yoshinori Akiyama

  GENES & GENETIC SYSTEMS, Dec. 2011

  
• 熱ショック転写因子シグマ32の細胞膜移行:フィードバック制御の必須段階

  YURA TAKASHI; RIMU BENTORE; GUROSU KYARORU; MORI HIROYUKI; ITO KOREAKI; AKIYAMA YOSHINORI

  日本遺伝学会大会プログラム・予稿集, 31 Aug. 2011

  
• 翻訳途上鎖のダイナミズムによる翻訳伸長の制御とそれを利用した蛋白質局在化モニタリング機構

  CHIBA SHINOBU; KANAMORI TAKASHI; UEDA TAKUYA; AKIYAMA YOSHINORI; POGLIANO KIT; ITO KOREAKI

  日本生体エネルギー研究会討論会講演要旨集, 2011

  
• タンパク質の分泌を促進するSecDF複合体の構造と機能

  MORI HIROYUKI; TSUKAZAKI TOMOYA; ECHIZEN YUKA; MACHIDA YUKIKO; AKIYAMA YOSHINORI; MATURANA ANDRES D; NUREKI OSAMU; ITO KOREAKI

  日本生体エネルギー研究会討論会講演要旨集, 2011

  
• 大腸菌表層ストレス応答に関与する膜内切断プロテアーゼRsePの生理的PDZリガンドの探索

  HIZUKURI YOHEI; AKIYAMA YOSHINORI

  生化学, 2011

  
• 蛋白質膜透過を促進する膜内在性因子SecDFの構造と機能

  MORI HIROYUKI; TSUKAZAKI TOMOYA; ECHIZEN YUKA; AKIYAMA YOSHINORI; NUREKI OSAMU; ITO KOREAKI

  生化学, 2011

  
• In vitrostudy of translation arrest of MifM, a regulatory nascent chain that monitors membrane protein biogenesis inBacillus subtilis.

  Chiba S; Kanomori T; Ueda T; Akiyama Y; Pogliano K; Ito K

  第34回日本分子生物学会年会 Symposium "Regulatory systems mediated by programmed ribosomal stalling". 横浜, 2011年12月13日-16日, 2011, Peer-reviewed

  
• Modularity and species-specificity of translation arrest motifs in translocation monitor proteins.

  Chiba S; Kanamori T; Ueda T; Akiyama Y; Pogliano K; Ito K

  Cold Spring Harbor Asia Conference "Protein Homeostasis in Health and Disease" Suzhou, China, September 26-30, 2011, 2011, Peer-reviewed

  
• Visualization of cellular polypeptidyl-tRNAs uncovers futile protein synthesis in E. coli

  Ito K; Chadani Y; Nakamori K; Chiba S; Akiyama Y; Abo T

  Cold Spring Harbor Asia Conference "Protein Homeostasis in Health and Disease" Suzhou, China, September 26-30, 2011, 2011, Peer-reviewed

  
• 翻訳途上鎖のダイナミズムによる翻訳伸長の制御とそれを利用した蛋白質局在化モニタリング機構

  千葉志信; 金森 崇; 上田卓也; 秋山芳展; Kit Pogliano; 伊藤維昭

  第3 7 回日本生体エネルギー研究会、京都、2011 年12 月20 日-22 日, 2011, Peer-reviewed

  
• 大腸菌表層タンパク質の品質管理に関わる新規プロテアーゼホモログの解析

  成田新一郎; 秋山芳展

  第8回 21世紀大腸菌研究会, 南木曽,2011 年5月19 日, 2011, Peer-reviewed

  
• タンパク質の分泌を促進するSecDF 複合体の構造と機能

  森 博幸; 塚崎智也; 越前友香; 町田裕紀子; 秋山芳展; Andres D. Maturana; 濡木 理; 伊藤維昭

  第37 回日本生体エネルギー研究会、京都、2011 年12 月20 日-22 日, 2011, Peer-reviewed

  
• 大腸菌表層ストレス応答に関与する膜内切断プロテアーゼRseP の生理的PDZ リガンドの探索

  檜作洋平; 秋山芳展

  第84 回日本生化学会大会, 京都 2011 年9 月21 日-24 日, 2011, Peer-reviewed

  
• 蛋白質膜透過を促進する膜内在性因子SecDF の構造と機能

  森 博幸; 塚崎智也; 越前友香; 秋山芳展; 濡木 理; 伊藤維昭

  第84 回日本生化学会大会シンポジウム「生体膜エネルギー変換装置の超分子科学」, 京都 2011 年9 月21 日-24 日, 2011, Peer-reviewed

  
• Function and regulation of RseP, the S2P family intramembrane protease of E. coli.

  Akiyama, Y; Hizukuri, Y

  International Union of Microbiological Societies 2011 Congress, Sapporo, Japan,, 2011

  
• 異常外膜タンパク質蓄積ストレスに応答する表層ストレス応答因子 σE の未知の活性化経路の可能性

  檜作洋平; 秋山芳展

  第８回２１世紀大腸菌研究会、南木曽, 2011

  
• 部位特異的in vivo光架橋実験法を用いたSecG近接因子の同定

  TANAKA NATSUKO; KOBAYASHI GEN; DOMAE NAOSHI; SUZUKI TAKEHIRO; ITO KOREAKI; AKIYAMA YOSHINORI; MORI HIROYUKI

  日本化学会講演予稿集, 12 Mar. 2010

  
• 大腸菌膜内切断プロテアーゼRsePのX線結晶構造解析

  SUZUKI MAMORU; INABA KENJI; MAEGAWA KEN'ICHI; AKIYAMA SHUJI; ITO KOREAKI; AKIYAMA YOSHINORI

  PFシンポジウム要旨集, 2010

  
• 大腸菌表層ストレス応答に関わるS2P膜内プロテアーゼRsePのPDZドメイン機能の解析

  HIZUKURI YOHEI; AKIYAMA YOSHINORI

  生化学, 2010

  
• タンパク質膜透過促進因子SecDFの機能解析

  MORI HIROYUKI; TSUKAZAKI TOMOYA; ECHIZEN YUKA; AKIYAMA YOSHINORI; NUREKI OSAMU; ITO KOREAKI

  日本生体エネルギー研究会討論会講演要旨集, 2010

  
• 膜結合型AAA⁺プロテアーゼFtsHの構造と機能

  SUNO RYOJI; SHIMOYAMA MASAKAZU; SHIMODATE NATSUKA; ABE AKIKO; AKIYAMA YOSHINORI; YOSHIDA MASASUKE

  日本生体エネルギー研究会討論会講演要旨集, 2010

  
• Structure, function and evolution of bacterial protein translocation machinery.

  Mori H; Tsukazaki T; Akiyama Y; Nureki O; Ito K

  第33 回日本分子生物学会年会・第83 回日本 生化学会大会合同大会 ワークショップ「進化からみたタンパク質社会」, 神戸, 2010 年12 月7 日-10日, 2010, Peer-reviewed

  
• The role of the mobile regions in the ATP-dependent protease activity of FtsH. The 3 「(I International Symposium on Protein Community, Nara, September 13-16,2010

  Suno R; Shimoyama M; Shimodate N; Abe A; Akiyama Y; Yoshida M

  The 3rd International Symposium on Protein Community, Nara, September 13-16,2010, 2010, Peer-reviewed

  
• A functionally important intramolecular interaction in SecA: involvement of hydrophobic amino acids in motif IV and anti-parallel beta sheet in translocase activation

  Mori H; Tsukazaki T; Nureki; Ito K; Akiyama Y

  The 3rd International Symposium on Protein Community, Nara, September 13-16, 2010, 2010, Peer-reviewed

  
• 大腸菌表層ストレス応答に関わるS2P膜内プロテアーゼRsePのPDZドメイン機能の解析

  檜作 洋平; 秋山芳展

  第３３回日本分子生物学会年会・第８３回日本生化学会大会合同大会、神戸, 2010

  
• 膜結合型ATP依存性プロテアーゼFtsHの構造と機能

  寿野良二; 下立夏香; 下山真和; 阿部明子; 秋山芳展; 吉田賢右

  日本生体エネルギー研究会、大阪、, 2010

  
• Roles of the two PDZ domains of RseP, the S2P family intramembrane protease of Escherichia coli, in regulation of its protease function.

  Hizukuri, Y; Akiyama, Y

  The 3rd International Symposium on Protein Community, Nara,, 2010

  
• The role of the mobile regions in the ATP-dependent protease activity of FtsH.

  Suno, R; Shimoyama, M; Shimodate, N; Abe, A; Akiyama, Y; Yoshida, M

  The 3rd International Symposium on Protein Community, Nara, 2010

  
• SRP-dependent targeting of sigma 32 to the membrane: A critical step for the chaperone-mediated feedback control in bacteria heat shock response.

  Yura, T; Lim, B; Gross, C; Ito, K; Mori, H; Akiyama, Y

  The 3rd International Symposium on Protein Community, Nara, 2010

  
• Visualizing dynamic "nascentome" of the cell.

  Ito, K; Chiba, S; Akiyama, Y; Abo, T

  FASEB Summer Research Conference "Protein Folding in the Cell", Saxtons River, U.S.A.,, 2010

  
• 細菌表層ストレス応答に関与する膜内プロテアーゼRsePのPDZドメインによる活性制御機構解析

  檜作 洋平; 秋山芳展

  特定領域研究「タンパク質の社会」若手ワークショップ、福岡, 2010

  
• 表層ストレス応答に関わる膜内プロテアーゼRsePのPDZドメインによる活性制御機構解析

  檜作 洋平; 秋山芳展

  第７回２１世紀大腸菌研究会、熊本, 2010

  
• New function of RseP, the E. coli S2P family protease.

  Akiyama, Y

  Gordon Research Conference on Proteolytic enzymes & their inhibitors, Lucca, Italy, 2010

  
• 大腸菌膜内切断プロテアーゼRsePのX線結晶構造解析

  鈴木守; 稲葉謙次; 前川憲一; 秋山修志; 伊藤維昭; 秋山芳展

  第27回PFシンポジウム、つくば, 2010

  
• 大腸菌膜内切断プロテアーゼRsePのPDZドメインの構造解析.

  鈴木守; 稲葉謙次; 前川憲一; 秋山修志; 伊藤維昭; 秋山芳展

  第23回日本放射光学会年会、姫路, 2010

  
• Fate of signal peptides in bacteria: post liberation cleavage by S2P protease

  SAITO AKIRA; CHIBA SHINOBU; MATSUO EIICHI; NISHIMURA OSAMU; ITO KOREAKI; AKIYAMA YOSHINORI

  生化学, 2010

  
• 膜内切断プロテアーゼRsePのPDZドメインの構造解析

  SUZUKI MAMORU; INABA KENJI; MAEGAWA KEN'ICHI; AKIYAMA SHUJI; ITO KOREAKI; AKIYAMA YOSHINORI

  日本放射光学会年会・放射光科学合同シンポジウム予稿集, 10 Dec. 2009

  
• A pair of circularly permutated PDZ domains control RseP, The S2P family intramembrane protease of E. coli.

  Inaba K; Suzuki M; Maegawa K.-i; Akiyama S; Ito K; Akiyama Y

  第 82 回日本生化学会大会、神戸、2 0 0 9 年1O 月21 日-24 日, 2009, Peer-reviewed

  
• 膜結合型ATP 依存性プロテアー ゼFtsH の基質分解モデルの生化学的検討

  寿野良二; 下立夏香; 下山真和; 秋山芳展; 吉田賢右

  第82 回日本生化学会大会、神戸、2009 年10 月21 日-24 日, 2009, Peer-reviewed

  
• 部位特異的 in vivo 光架橋実験法を用いたSecG 近接因子の同定

  田中夏子; 小林元; 堂前直; 鈴木健裕; 伊藤維昭; 秋山芳展; 森博幸

  第6 回21 世紀大腸菌研究会, 静岡, 2009 年6 月11 日-12 日, 2009, Peer-reviewed

  
• 蛋白質の一生

  徳永文稔; 門脇寿枝; 西頭英起; 内木宏延; 樋口京一; 中矢正; 鈴木利治; 後藤祐児; 船津高志; 金子清俊; 中戸川仁; 森和俊; 米田悦啓; 垣塚彰; 馬艶; 久野高義; 和田郁夫; 西川周一; 伊川正人; 吉田賢右; 遠藤斗志也; 永田和宏; 阿部哲也; 養王田正文; 金城政孝; 元島史尋; 田口英樹; 吉田祐美; 中村暢宏; 阪口雅郎; 小椋光; 森正敬; 稲葉謙次; 久保田広志; 森戸大介; 細川暢子; 大塚健三; 秋山芳展; 徳田元; 藤木幸夫

  蛋白質 核酸 酵素, 10 Jun. 2008

  
• SecM の分泌は低温感受性の性 をもつ

  武藤洋樹; 森博幸; 秋山芳展; 伊藤維昭

  大大学白研究所セミナー 「 白 の白 の と 抻入の分子メカニズムーその核心と 抻入の分子メカニズムーその核心にる」吹田 2008 年1 月24-25 日, 2008, Peer-reviewed

  
• Function and regulation of E. coli membrane metalloproteases.

  Akiyama Y

  Gordon Research Conference on Matrix metalloproteinases. June 3-8, Lucca, Italy, 2007, 2007, Peer-reviewed

  
• Substrate specificity and reaction environment of GlpG, an E. coli intramembrane-cleaving protease.

  Akiyama, Y

  International Symposium "Milestones in the Life of Proteins" 16 March, Kyoto 2007, 2007, Peer-reviewed

  
• 大腸菌Rhomboid ファミリー膜プロテアーゼ GlpG の作用メカニズム

  前河早希; 小出佳代; 伊藤維昭; 秋山芳展

  第4 回 21 世紀大腸菌研究会 藤枝 2007, 2007, Peer-reviewed

  
• 膜プロテアーゼによる膜タンパク質の品質管理と機能制御

  秋山芳展

  京都大学ウイルス研究所学術講演会 京都 2007, 2007, Peer-reviewed

  
• Regulatory and reaction mechanisms of E. coli RIP proteases.

  Akiyama Y; Maegawa S; Koide K; Ito K

  The Ringberg Symposium on Regulated Intramembrane Proteolysis. November, Rottach-Egern, Germany, 2006, 2006, Peer-reviewed

  
• Mutations in secY alter insertion and folding of lactose permease in the membrane of E. coli.

  Nagamori S; Shimohata N; Akiyama Y; Kaback H.R; Ito K

  Gordon Research Conference on Bacteria Cell Surfaces. June 25-30 2006, Colby-Sawyer College, New London, NH USA,2006, 2006, Peer-reviewed

  
• Protein quality control devices in the Escherichia coli plasma membrane.

  Ito K; Shimohata N; Nagamori S; Kaback H.R; Sakoh M; Chiba S; Mori H; Akiyama Y

  20th IUBMB International Congress of Biochemistry and Molecular Biology. Symposium Quality Control of Proteins and Proteolysis, Abstract p. 75, Jun 18-23,2006, 2006, Peer-reviewed

  
• Membrane-proximal proteolysis 膜近傍におけるプロテオリシス研究の最先端

  秋山芳展

  蛋白質研究所セミナ、吹田、2006, 2006, Peer-reviewed

  
• 大腸菌RIP (regulated intramembrane proteolysis) プロテアーゼRseP の機能と制御

  秋山芳展; 小出佳代; 前河早希; 伊藤維昭

  第6回日本蛋白質科学会年会ワークショップ「水と蛋白質が織り成す機能発現のメカニズム」. 要旨集P.23, 京都,2006, 2006, Peer-reviewed

  
• 大腸菌FtsH 及びその超分子複合体の電子顕微鏡構造解析から予測される分解調節機構

  才川直哉; 鈴木博文; 秋山芳展; 伊藤維昭; 木村能章

  第6回日本蛋白質科学会年会ワークショップ「AAA+タンパク質の高次構造と作用のダイナミクス」. 要旨集p. 18, 京都,2006, 2006, Peer-reviewed

  
• 大腸菌RseP プロテアーゼの活性部位が存在する環境

  小出佳代; 前河早希; 伊藤維昭; 秋山芳展

  第3回21 世紀大腸菌研究会「モデル生物大腸菌の統合的理解をめざして」. 要旨集P.10, 近江八幡,2006, 2006, Peer-reviewed

  
• 3P131 The three-dimensional structure of FtsH oligomer from E. coli by cryonegative electron-microscopy

  Saikawa N; Suzuki H; Akiyama Y; Ito K; Kimura Y

  Seibutsu Butsuri, 19 Oct. 2005, Peer-reviewed

  
• Regulatory and reaction mechanisms of E. coli RIP (regulated intramembrane proteolysis) proteases.

  Akiyama Y; Kanehara K; Maegawa S; Koide K; Ito K

  International Symposium on Life of Proteins. Maturation, Translocation and Quality Control in the Cell. 10.30-11.3, Awajisima, Hyogo, Japan, 2005, 2005, Peer-reviewed

  
• Coordinated functions of SecA-SecYEG protein translocation system.

  Ito K; Shimohata N; Nakatogawa H; Murakami A; Muto H; Akiyama Y; Tsukazaki T; Mori H

  International Symposium on Life of Proteins. Maturation, Translocation and Quality Control in the Cell. 10.30-11.3, Awajisima, Hyogo, Japan, 2005, 2005, Peer-reviewed

  
• The three-dimensional structure of FtsH oligomer from E. coli by cryo-staining electron microscopy.

  Saikawa N; Suzuki H; Akiyama Y; Ito K; Kimura Y

  The Sixth International Conference on AAA Proteins. September 14-18, Graz, Austria, 2005, 2005, Peer-reviewed

  
• Stress response devices in the E. coli plasma membrane and their ability to sense abnormality of integral membrane proteins.

  Ito K; Shimohata N; Mori H; Kanehara K; Akiyama Y

  IUMS 2005. XI International Congress of Bacteriology and Applied Microbiology. Symposium "Envelope Stress". Abstracts p. 214-215. July 23-28, San Francisco, California, USA, 2005, 2005, Peer-reviewed

  
• 大腸菌のRhomboid ファミリープロテアーゼGlpG の機能

  前河早希; 伊藤維昭; 秋山芳展

  ２１ 世紀大腸菌研究会 「モデル生物大腸菌の統合的理解にむけて」 予稿集p.19. 6/23-24, 三重県志摩市, 2005, 2005, Peer-reviewed

  
• 電子顕微鏡による大腸菌膜プロテアーゼ FtsH の構造解析

  才川直哉; 鈴木博文; 秋山芳展; 伊藤維昭; 木村能章

  第２８回日本分子生物学会年会 ワークショップ「細胞機能のキープレイ ヤーAAA+タンパク質の世界」 要旨集p. 45. 12.7-10, 福岡, 2005, 2005, Peer-reviewed

  
• 膜蛋白質の形成におけるトランスロコンの機能と機能不全感知システム

  伊藤維昭; 森博幸; 下畑宣行; 秋山芳展

  第２８回日本分子生物学会年会 ワークショップ「タンパク質と脂質の オーケストレーションによる膜の形成」 要旨集 p. 112. 12.7-10, 福岡, 2005, 2005, Peer-reviewed

  
• 表層ストレス応答に関わる大腸菌膜プロテアーゼ RseP (YaeL) の活性制御機構

  秋山芳展; 金原和江; 小出佳代; 伊藤維昭

  第２８回日本分子生物学会年会 シンポジウム「小胞体ストレ スとタンパク質の品質管理」 要旨集p. 24. 12.7-10, 福岡, 2005, 2005, Peer-reviewed

  
• 大腸菌FtsH の電子顕微鏡解析

  才川直哉; 鈴木博文; 木村能章; 秋山芳展; 伊藤維昭

  第５回日 本蛋白質科学会年会. 予稿集p. 104. 6.30-7.2, 福岡, 2005, 2005, Peer-reviewed

  
• FtsH protease

  Yoshinori Akiyama; Koreaki Ito; Teru Ogura

  Handbook of Proteolytic Enzymes: Second Edition, 30 Jun. 2004

  
• 大腸菌の表層蛋白質の品質管理 (細胞における 蛋白質の一生--生成・成熟・輸送・管理・分解・病態) -- (蛋白質の品質管理)

  秋山 芳展

  蛋白質核酸酵素, May 2004

  
• Roles of Escherichia coli Integral Membrane Proteases in Membrane Quality Control and Stress Responses

  CHIBA Shinobu; AKIYAMA Yoshinori

  化学と生物, 25 Feb. 2004

  
• 1SA04 Function of YaeL, an E. coli membrane protease involved in extracytoplasmic stress response

  Akiyama Y; Kanehara K; Ito K

  Seibutsu Butsuri, 2004

  
• Roles of Escherichia coli Integral Membrane Proteases in Membrane Quality Control and Stress Responses

  CHIBA Shinobu; AKIYAMA Yoshinori

  KAGAKU TO SEIBUTSU, 2004

  
• 膜内部での蛋白質切断(RIP)によるシグナル分子の生成--細菌から高等生物まで

  金原 和江; 秋山 芳展

  蛋白質核酸酵素, Jun. 2003

  
• Quality control of membrane proteins by AAA protease in Escherichia coli

  SAIKAWA Naoya; AKIYAMA Yoshinori

  Bioscience & industry, 01 May 2003

  
• Proton-motive force stimulates the proteolytic activity of FtsH, a membrane-bound ATP-dependent protease in Escherichia coli

  Y Akiyama

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Jun. 2002

  
• FtsHによる膜蛋白質分解開始機構 N‐terminal tailの長さの認識

  千葉志信; 秋山芳展; 森博幸; 松尾英一; 伊藤維昭

  生化学, 25 Aug. 2000

  
• FtsHのプロテアーゼ活性発現における膜結合領域の役割

  秋山芳展; 木原章雄; 伊藤維昭

  日本分子生物学会年会プログラム・講演要旨集, 22 Nov. 1999

  
• Roles of the periplasmic domain of the Escherichia coli AAA protease FtsH (HflB) in protein interactions and activity modulation

  AKIYAMA Yoshinori; KIHARA Akio; MORI Hiroyuki; ITO Koreaki

  日本分子生物学会年会プログラム・講演要旨集, 01 Dec. 1998

  
• 大腸菌膜結合型AAAプロテアーゼFtsHのペリプラスム領域の機能の解析

  秋山芳展; 木原章雄; 森博幸; 伊藤維昭

  日本分子生物学会年会プログラム・講演要旨集, Nov. 1998

  
• Differential in vivo roles played by DsbA and DsbC in the formation of protein disulfide bonds. (vol 272, pg 10349, 1997)

  M Sone; Y Akiyama; K Ito

  JOURNAL OF BIOLOGICAL CHEMISTRY, Oct. 1998

  
• Use of YccA, a new membrane-bound substrate of FtsH, to study regulatory mechanisms of the FtsH protease.

  KIHARA AKIO; AKIYAMA YOSHINORI; ITO KOREAKI

  日本分子生物学会年会プログラム・講演要旨集, Dec. 1997

  
• Role of SecYEG in orienting a model protein with both signal and transmembrane sequences in the membrane.

  NITTA SHUJI; AKIYAMA YOSHINORI; SHIRAI YOSHINORI; ITO KOREAKI

  日本分子生物学会年会プログラム・講演要旨集, Dec. 1997

  
• Role of periplasmic domain 1 of SecY in protein translocation.

  HONMA TAKAYUKI; MORI HIROYUKI; MATSUMOTO GEN; YOSHIHISA TOORU; NITTA SHINJI; MATSUO EIICHI; AKIYAMA YOSHINORI; ITO KOREAKI

  日本分子生物学会年会プログラム・講演要旨集, Dec. 1997

  
• 原核生物のATP依存性プロテア-ゼによる細胞機能調節 (プロテオリシス--蛋白質分解の分子機構とバイオロジ-) -- (選択的蛋白質分解の分子機構)

  小椋 光; 秋山 芳展

  蛋白質核酸酵素, Oct. 1997

  
• Control of Cellular Functions by ATP-Dependent Proteases in Prokaryotes.

  OGURA TERU; AKIYAMA YOSHINORI

  蛋白質 核酸 酵素, Oct. 1997

  
• Host regulation of lysogenic decision in bacteriophage lambda: Transmembrane modulation of FtsH (HflB), the cII degrading protease, by HflKC (HflA)

  A Kihara; Y Akiyama; K Ito

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, May 1997

  
• Intracellular stability of alpha fragments of beta-galactosidase: Effects of amino-terminally fused polypeptides (vol 215, pg 452, 1995)

  T Homma; T Yoshihisa; A Kihara; Y Akiyama; K Ito

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Oct. 1996

  
• AAAスーパーファミリー蛋白 - 新しいATPaseファミリーの構造類似性とその多様な細胞機能

  小椋 光; 秋山 芳展; 多賀谷 光男; 塚本 利朗

  日本分子生物学会年会プログラム・講演要旨集, Aug. 1996

  
• DsbAによる誤ったジスルフィド結合形成の促進 : 変異型アルカリ性ホスファターゼ(PhoA)を用いた解析

  曾根 道夫; 岸上 哲士; 吉久 徹; 秋山 芳展; 伊藤 維昭

  日本分子生物学会年会プログラム・講演要旨集, 01 Aug. 1996

  
• SecYはSecEとSecGに対するインターフェイスを持つ

  本間 貴之; 吉久 徹; 松尾 英一; 秋山 芳展; 伊藤 維昭

  日本分子生物学会年会プログラム・講演要旨集, 01 Aug. 1996

  
• SecYEGプロテオリポソームへの膜タンパク質の組み込み

  白井 良憲; 秋山 芳展; 吉久 徹; 伊藤 維昭

  日本分子生物学会年会プログラム・講演要旨集, 01 Aug. 1996

  
• FtsHのHflKCとの複合体形成及び活性調節

  木原 章雄; 秋山 芳展; 吉久 徹; 伊藤 維昭

  日本分子生物学会年会プログラム・講演要旨集, 01 Aug. 1996

  
• Structure and Functions of the AAA Superfamily Proteins.

  OGURA TERU; TAGAYA MITSUO; TOMOYASU TOSHIFUMI; ITO KOREAKI; AKIYAMA YOSHINORI; NAKAI TOSHIKI; OSUMI TAKASHI; TSUKAMOTO TOSHIRO

  細胞工学, Jul. 1996

  
• A built-in of a film protein to SecYEG proteo ribosome.

  SHIRAI YOSHINORI; AKIYAMA YOSHINORI; YOSHIHISA TOORU; ITO KOREAKI

  日本分子生物学会年会プログラム・講演要旨集, Jul. 1996

  
• AAA super family protein - structural similarity of new ATPase family and various cell functions.

  OGURA AKIRA; AKIYAMA YOSHINORI; TAGAYA MITSUO; TSUKAMOTO TOSHIAKI

  日本分子生物学会年会プログラム・講演要旨集, Jul. 1996

  
• Erratum: Intracellular stability of α fragments of β-galactosidase: Effects of amino-terminally fused polypeptides (Biochemical and Biophysical Research Communications (1995) 215 No 2 (452-458))

  T. Homma; T. Yoshihisa; A. Kihara; Y. Akiyama; K. Ito

  Biochemical and Biophysical Research Communications, 01 Jan. 1996

  
• FtsH is required for proteolytic elimination of uncomplexed forms of SecY, an essential protein translocase subunit

  Akio Kihara; Yoshinori Akiyama; Koreaki Ito

  Proceedings of the National Academy of Sciences of the United States of America, 09 May 1995

  
• A CYTOPLASMIC DOMAIN IS IMPORTANT FOR THE FORMATION OF A SECY-SECE - TRANSLOCATOR COMPLEX

  T BABA; T TAURA; T SHIMOIKE; Y AKIYAMA; T YOSHIHISA; K ITO

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, May 1994

  
• INVOLVEMENT OF FTSH IN PROTEIN ASSEMBLY INTO AND THROUGH THE MEMBRANE .2. DOMINANT MUTATIONS AFFECTING FTSH FUNCTIONS

  Y AKIYAMA; Y SHIRAI; K ITO

  JOURNAL OF BIOLOGICAL CHEMISTRY, Feb. 1994

  
• CELL-GROWTH AND LAMBDA-PHAGE DEVELOPMENT CONTROLLED BY THE SAME ESSENTIAL ESCHERICHIA-COLI GENE, FTSH HFLB

  C HERMAN; T OGURA; T TOMOYASU; S HIRAGA; Y AKIYAMA; K ITO; R THOMAS; R DARI; P BOULOC

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Nov. 1993

  
• MEMBRANE-COMPONENTS OF THE PROTEIN SECRETION MACHINERY

  K ITO; Y AKIYAMA

  METHODS IN CELL BIOLOGY, 1991

  
• A POSITIVELY CHARGED REGION IS A DETERMINANT OF THE ORIENTATION OF CYTOPLASMIC MEMBRANE-PROTEINS IN ESCHERICHIA-COLI

  K YAMANE; Y AKIYAMA; K ITO; S MIZUSHIMA

  JOURNAL OF BIOLOGICAL CHEMISTRY, Dec. 1990

  
• COMPLEMENTATION OF THE PROTEIN-TRANSPORT DEFECT OF AN ESCHERICHIA-COLI SEC-Y MUTANT (SECY24) BY BACILLUS-SUBTILIS-SEC-Y HOMOLOG

  K NAKAMURA; H TAKAMATSU; Y AKIYAMA; K ITO; K YAMANE

  FEBS LETTERS, Oct. 1990

  
• タンパク質膜透過に関与する大腸菌内在性膜タンパク質SecY

  秋山 芳展

  生化学, Aug. 1989

  
• INVOLVEMENT OF HTPR GENE-PRODUCT (SIGMA-32) IN REPLICATION OF MINI-F PLASMID

  C WADA; Y AKIYAMA; K ITO; T YURA

  JAPANESE JOURNAL OF GENETICS, Dec. 1985

  

Books and Other Publications

• FtsH

  Teru Ogura; Tkashi Okuno; Ryoji Suno; Yoshinori Akiyama, Contributor

  Elsevier Ltd, 2013, Not refereed

  
• HtpX

  Yoshinori Akiyama; Koreaki Ito, Contributor

  Elsevier Ltd, 2013, Not refereed

  
• RseP

  Yohei Hizukuri; Koraki Ito; Yoshinori Akiyama, Contributor

  Elsevier Ltd, 2013, Not refereed

  

Awards

• 01 Apr. 2019

  公益財団法人長瀬科学技術振興財団, 長瀬研究振興賞

External funds: Kakenhi

• Physiological functions and selective substrate-cleavage mechanism of E. coli intramembrane protease RseP

  Grant-in-Aid for Scientific Research (B)

  Basic Section 43030:Functional biochemistry-related

  Kyoto University

  Yoshinori Akiyama

  From 01 Apr. 2018, To 31 Mar. 2021, Project Closed

  膜内切断プロテアーゼ;大腸菌;切断基質;膜内部でのタンパク質切断;S2Pプロテアーゼ;Fecオペロン;鉄取り込み;蛍光ペプチド;速度論的解析;小分子膜タンパク質;RseP;Toxin-antitoxin;HokB;Persister;small membrane protein;FRET;生体膜;プロテアーゼ;膜内切断;蛍光基質

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18H02404/
• Molecular mechanism for quality control of bacterial outer membrane by the periplasmic protease BepA

  Grant-in-Aid for Scientific Research (C)

  Morioka College

  Shin-ichiro Narita

  From 01 Apr. 2015, To 31 Mar. 2018, Project Closed

  細胞内タンパク質分解;表層ストレス応答;細菌細胞表層;プロテアーゼ;シャペロン;外膜タンパク質;ジスルフィド結合;タンパク質間相互作用

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K07008/
• Mechanism of selective substrate cleqvage and novel cellular functions ofintramembrane protease RseP

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Yoshinori Akiyama

  From 01 Apr. 2015, To 31 Mar. 2018, Project Closed

  生体膜;プロテアーゼ;膜内切断;表層ストレス応答;膜タンパク質;S2P;細菌;ストレス応答;膜プロテアーゼ;大腸菌;構造生物学

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15H04350/
• ビブリオ菌における新生鎖機能を介したタンパク質膜透過の制御

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Biological Sciences

  Kyoto University

  秋山 芳展

  From 01 Apr. 2015, To 31 Mar. 2017, Project Closed

  翻訳停止;リボソーム;新生鎖;ビブリオ菌;SecDF;タンパク質;翻訳;細菌;VemP

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-15H01532/
• Elucidation of substrate recognition mechanism of I-CLiP in the trans- and juxta-membrane regions

  Grant-in-Aid for Scientific Research (B)

  Yokohama City University

  Terukazu NOGI

  From 01 Apr. 2014, To 31 Mar. 2018, Project Closed

  タンパク質;シグナル伝達;酵素;膜タンパク質;立体構造解析;蛋白質;膜蛋白質

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26291016/
• Analysis of a chaperone/protease involved in quality control of bacterial cell surface

  Grant-in-Aid for Scientific Research (C)

  Morioka College;Kyoto University

  Shin-ichiro NARITA

  From 01 Apr. 2012, To 31 Mar. 2015, Project Closed

  細菌細胞表層;表層ストレス応答;σE;外膜タンパク質;リポ多糖;プロテアーゼ;ジスルフィド結合;大腸菌;タンパク質品質管理;シグマE;BAM複合体

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24570152/
• A novel regulation mechanism of cellular functions by intramembrane proteolysis

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Yoshinori AKIYAMA

  From 01 Apr. 2012, To 31 Mar. 2015, Project Closed

  大腸菌;膜プロテアーゼ;膜内タンパク質切断;表層ストレス応答;S2Pプロテアーゼ;基質探索;PDZドメイン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24370054/
• Degradation mechanisms of sigma 32 by membrane targeting via SRP pathway

  Grant-in-Aid for Challenging Exploratory Research

  Kyoto University

  Hiroyuki MORI

  From 01 Apr. 2011, To 31 Mar. 2013, Project Closed

  生体膜;σ32;SRP;Ffh;SecY;FtsH;熱ショック応答;分子シャペロン;シグマ３２

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23657128/
• SecDF function and roles of the proton motive force in protein translocation

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Hiroyuki MORI

  From 01 Apr. 2010, To 31 Mar. 2013, Project Closed

  生体膜;SecDF;膜透過;in vivo光架橋実験;イオントランスポーター;SecYEG;細菌;プロトン駆動力;SecA;タンパク質膜透過;SecA ATPase;結晶構造解析;カチオントランスポーター

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22370070/
• X-ray Crystallographic analysis of membrane-bound AAA+ protease FtsH

  Grant-in-Aid for Young Scientists (B)

  Kyoto Sangyo University;Tokyo Institute of Technology

  Ryoji SUNO

  From 01 Apr. 2009, To 31 Mar. 2011, Project Closed

  X線結晶構造解析;AAA+タンパク質;ATP依存性プロテアーゼ;超分子複合体;酵素;プロテアーゼ;タンパク質;AAA+ファミリータンパク質;酵素反応;構造解析;X線構造解析;ATP加水分解;品質管理

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770106/
• 膜内切断(RIP)プロテアーゼの細胞機能

  Grant-in-Aid for Scientific Research on Priority Areas

  Biological Sciences

  Kyoto University

  千葉 志信

  From 01 Apr. 2009, To 31 Mar. 2011, Project Closed

  膜蛋白質分解;プロテアーゼ;原核生物;分子生物学;分泌蛋白質

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-21025016/
• Analysis of the membrane stress response in E. coli

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Yoshinori AKIYAMA

  From 01 Apr. 2008, To 31 Mar. 2011, Project Closed

  生体膜;膜タンパク質;表層ストレス応答;タンパク質分解;RIP;プロテアーゼ;RseP;PDZドメイン;シグマE;品質管理;分子シャペロン;シグナルペプチ;枯草菌;大腸菌;分解;シャペロン;SecY;膜組み込み

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20370078/
• Nascent chain biology

  Grant-in-Aid for Scientific Research (A)

  Kyoto Sangyo University;Kyoto University

  Koreaki ITO

  From 01 Apr. 2008, To 31 Mar. 2013, Project Closed

  翻訳;翻訳制御;リボソーム;翻訳伸長停止配列;アレストペプチド;SecM;MifM;ナッセントーム;polypeptidyl-tRNA;シャペロン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20247020/
• Quality control of E. coli membrane proteins

  Grant-in-Aid for Scientific Research on Priority Areas

  Biological Sciences

  Kyoto University

  Yoshinori AKIYAMA

  From 01 Apr. 2007, To 31 Mar. 2012, Project Closed

  膜タンパク質;品質管理;膜プロテアーゼ;ストレス応答;タンパク質膜透過;膜内切断プロテアーゼ;ロンボイド;基質特異性;大腸菌;RIP;表層ストレス応答;SecDF;プロトン駆動力;purotea-ze

  https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-19058007/
• 大腸菌膜内切断(RIP)プロテアーゼの細胞機能と制御

  Grant-in-Aid for Scientific Research on Priority Areas

  Biological Sciences

  Kyoto University

  秋山 芳展

  From 01 Apr. 2007, To 31 Mar. 2009, Project Closed

  膜内切断;プロテアーゼ;RIP;S2P;機能調節;PDZドメイン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19044020/
• Molecular mechanism ofreguLaled inframembrane proteolysis (RIP)

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Yoshinori AKIYAMA

  From 01 Apr. 2005, To 31 Mar. 2008, Project Closed

  膜内切断;プロテアーゼ;RIP;S2P;Rhomboid;結晶構造;活性部位環境;基質結合;機能調節;膜タンパク質;タンパク質分解;I-CLiP;システインスキャニング;チオール修飾;膜内タンパク質切断;ストレス応答;大腸菌, intramembrane proteolysis;protease;RIP;S2P;Rhomboid;Crystal structure;active site environment;substrate binding

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17370068/
• Control of extracytoplasmic stress responses by regulated intramembrane proteolysis

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Yoshinori AKIYAMA

  From 01 Apr. 2003, To 31 Mar. 2005, Project Closed

  プロテアーゼ;膜タンパク質;RIP;表層ストレス応答;大腸菌;タンパク質分解;ストレス応答;膜プロテアーゼ, protease;membrane proteins;regulated intramembrane proteolysis

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15370084/
• Cellular systems that control protein dynamism across the membrane

  Grant-in-Aid for Scientific Research on Priority Areas

  Biological Sciences

  Kyoto University

  Koreaki ITO

  From 01 Apr. 2002, To 31 Mar. 2007, Project Closed

  蛋白質膜透過;トランスロコン;SecY;SecA;翻訳アレスト;プロテアーゼ;ストレスレスポンス;ジスルフィド結合;膜蛋白質;トンスロコン;タンパク質膜透過;トランスコロン;蛋白質膜透渦, protein translocation across membrane;translocon;SecY;SecA;translation arrest;protease;stress response;disulfide bond

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14037231/
• Regulation mechanism of the DsbA/DsbB system that catalyzes disulfine bond formation

  Grant-in-Aid for Scientific Research (B)

  KYOTO UNIVERSITY

  Koreaki ITO

  From 01 Apr. 2000, To 31 Mar. 2002, Project Closed

  ジスルフィド結合;呼吸鎖;レドックス制御;ペリプラズム;大腸菌;Protein folding;タンパク質フォールディング;キノン;膜タンパク質;DsbA;DsbB, disulfide bond;Respiratory chain;Redox regulation;Periplasm;E.coli;protein folding

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12480188/
• Membrane protein degradation by E. coli FtsH

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Yoshinori AKIYAMA

  From 01 Apr. 1999, To 31 Mar. 2002, Project Closed

  大腸菌;FtsH;ATPase;protein degradation;膜タンパク質;AAAファミリー;Protein degradation;プロテアーゼ;膜ポテンシャル;膜貫通配列, E. coli;FtsH;ATPase;Protein degradation;membrane protein;AAA family

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680697/
• 膜結合型プロテアーゼ複合体とその多様な機能:大腸菌FtsH複合体の解析

  Grant-in-Aid for Scientific Research on Priority Areas (A)

  Kyoto University

  秋山 芳展

  From 01 Apr. 1998, To 31 Mar. 1999, Project Closed

  FtsH;HflKC;タンパク質分解

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10163222/
• Factors involved in the stop transfer process in membrane protein biogenesis

  Grant-in-Aid for Scientific Research (C)

  KYOTO UNIVERSITY

  Yoshinori AKIYAMA

  From 01 Apr. 1997, To 31 Mar. 1999, Project Closed

  FisH;ストップトランスファー;膜タンパク質;FtsH;SecY;prlA, FtsH;stop tranfer;membrane proteins

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680691/
• SecY functions that support the dynamic movement of SecA, a protein-translocating ATPase

  Grant-in-Aid for Scientific Research (B)

  KYOTO UNIVERSITY

  Koreaki ITO

  From 01 Apr. 1997, To 31 Mar. 2000, Project Closed

  蛋白質膜透過;SecY;SecA;膜蛋白質;蛋白質相互作用;ATPase, protein translocation;SecY;SecA;membrane protein;protein-protein interaction

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09480150/
• 膜結合型プロテアーゼ複合体とその多様な機能:大腸菌Frsh複合体の解析

  Grant-in-Aid for Scientific Research on Priority Areas

  Kyoto University

  秋山 芳展

  From 01 Apr. 1997, To 31 Mar. 1998, Project Closed

  タンパク質分解;FtsH;膜タンパク質;HflKC;SecY;溶原化;ATPase

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09267219/
• 蛋白質の機造構築におけるジスルフィド結合の役割とその形成機構

  Grant-in-Aid for Scientific Research on Priority Areas

  Kyoto University

  伊藤 維昭

  From 01 Apr. 1997, To 31 Mar. 1998, Project Closed

  ジスルフィド結合;アルカリ性ホスファターゼ;Dsb因子;呼吸鎖;電子伝達系;タンパク質折り畳み

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09261217/
• 膜結合プロテアーゼ複合体とその多様な機能:大腸菌FtsH複合体の解析

  Grant-in-Aid for Scientific Research on Priority Areas

  Kyoto University

  秋山 芳展

  From 01 Apr. 1996, To 31 Mar. 1997, Project Closed

  FtsH;ATPase;AAAファミリー;HflK;HflC;タンパク質分解;λcII

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08278216/
• Quality control of an integral membrane protein, SecY.

  Grant-in-Aid for Scientific Research (C)

  KYOTO UNIVERSITY

  Yoshinori AKIYAMA

  From 01 Apr. 1995, To 31 Mar. 1997, Project Closed

  FtsH;ATPase;AAAファミリー;膜タンパク質;タンパク質分解;SecY;F_0α;クオリティーコントロール;F_0a;大腸菌, FtsH;ATPase;AAA family;membrane proteins;protein degradation;SecY;F0 a;quality control

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680682/
• Cellular factors that assist in membrane protein anchoring

  Grant-in-Aid for international Scientific Research

  KYOTO UNIVERSITY

  Koreaki ITO

  From 01 Apr. 1995, To 31 Mar. 1997, Project Closed

  膜タンパク質;FtsH;SecY;タンパク質膜透過;分泌;大腸菌;蛍光標識;タンパク質分解;分子シャペロン, membrane protein;FtsH;Sec Y;protein translocation;secretion;Escherichia coli;fluorescent label;proteolysis

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044197/
• 大腸菌の新しいATPase相同体(FtsH)の膜タンパク質アンカリングにおける役割

  Grant-in-Aid for General Scientific Research (C)

  Kyoto University

  秋山 芳展

  From 01 Apr. 1993, To 31 Mar. 1995, Project Closed

  膜タンパク質;膜透過停止配列;ATPase;シャペロン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05680611/
• ペリプラズムにおけるタンパク質folding因子PpfAの構造と機能

  Grant-in-Aid for General Scientific Research (C)

  Kyoto University

  秋山 芳展

  From 01 Apr. 1992, To 31 Mar. 1993, Project Closed

  大腸菌;ペリプラズム;ジスルフィドボンド;DsbA;タンパク質フォールディング

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04833016/
• Dissection of polypeptide translocator function of SecY

  Grant-in-Aid for General Scientific Research (A)

  KYOTO UNIVERSITY

  Koreaki ITO

  From 01 Apr. 1990, To 31 Mar. 1993, Project Closed

  タンパク質分泌;タンパク質膜透過;SecY;SecE;優性変異;ペリプラズム;膜タンパク質;タンパク質のおりたたみ;ジスルフィド結合;シグナルペプチド;プロテア-ゼ;分泌;HNS;サプレッサ-, Protein secretion;protein translocation;SecY;SecE;Dominant mutation;Periplasm;Membrane protein

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02404087/
• 大腸菌BepAによる外膜タンパク質トリアージ機構とその制御

  Grant-in-Aid for Scientific Research (B)

  Basic Section 43030:Functional biochemistry-related

  Kyoto University

  秋山 芳展

  From 01 Apr. 2022, To 31 Mar. 2026, Granted

  大腸菌外膜;膜タンパク質分解;タンパク質構造;タンパク質機能制御;タンパク質品質管理;外膜タンパク質;表層プロテアーゼ;トリアージ;分子シャペロン;大腸菌

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H02571/
• Mechanism and regulation of the outer membrane protein triage by E. coli BepA

  Grant-in-Aid for Scientific Research (B)

  Basic Section 43030:Functional biochemistry-related

  Kyoto University

  秋山 芳展

  From 01 Apr. 2022, To 31 Mar. 2026, Granted

  大腸菌外膜;膜タンパク質分解;タンパク質構造;タンパク質機能制御;タンパク質品質管理;外膜タンパク質;表層プロテアーゼ;トリアージ;分子シャペロン;大腸菌

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K23835/

External funds: others

• Novel function of bacterial extracytoplasmic stress responses

  公益財団法人発酵研 究所助成事業

  From 01 Apr. 2012, To 31 Mar. 2013

  秋山芳展
• Mechanism of biogenesis of LptD, the LPS translocon in E. coli.

  公益財団法人長瀬科学技術振興財団2019年度研究助成

  From 01 Apr. 2019, To 31 Mar. 2020

  秋山芳展

Education

Teaching subject(s)

• From 01 Apr. 2024, To 31 Mar. 2025

  ILAS Seminar

  Z001, Spring, Institute for Liberal Arts and Sciences, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Biochemistry IIC

  7198, Spring, Graduate School of Science, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Biochemistry IIB

  7197, Fall, Graduate School of Science, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Biochemistry IIA

  7196, Spring, Graduate School of Science, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Biochemistry IID

  7199, Fall, Graduate School of Science, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar on Biochemistry IIA

  7092, Spring, Graduate School of Science, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar on Biochemistry IID

  7095, Fall, Graduate School of Science, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar on Biochemistry IIC

  7094, Spring, Graduate School of Science, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar on Biochemistry IIB

  7093, Fall, Graduate School of Science, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Biochemistry III

  8602, Fall, Faculty of Science, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Exercises in Biochemistry

  3628, Fall, Faculty of Science, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Dynamic aspects of proteins in the cell

  7033, Fall, Graduate School of Science, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Advanced Biochemistry IIC

  7198, Spring, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Advanced Biochemistry IIB

  7197, Fall, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Advanced Biochemistry IIA

  7196, Spring, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Advanced Biochemistry IID

  7199, Fall, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Biochemistry IIA

  7092, Spring, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Biochemistry IID

  7095, Fall, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Biochemistry IIC

  7094, Spring, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Biochemistry IIB

  7093, Fall, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Biochemistry III

  8602, Fall, Faculty of Science, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Exercises in Biochemistry

  3628, Fall, Faculty of Science, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Biochemistry IID

  7199, Fall, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Biochemistry IID

  7095, Fall, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Biochemistry III

  8602, Fall, Faculty of Science, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Biochemistry IIC

  7198, Spring, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Biochemistry IIB

  7197, Fall, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Biochemistry IIA

  7092, Spring, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Biochemistry IIA

  7196, Spring, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Biochemistry IIC

  7094, Spring, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Biochemistry IIB

  7093, Fall, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Exercises in Biochemistry

  3628, Fall, Faculty of Science, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Dynamic aspects of proteins in the cell

  7033, Fall, Graduate School of Science, 2
• From Apr. 2011, To Mar. 2012

  生物化学ゼミナールⅡA

  Spring, 理学研究科
• From Apr. 2011, To Mar. 2012

  生物化学ゼミナールⅡB

  Fall, 理学研究科
• From Apr. 2011, To Mar. 2012

  生物化学ゼミナールⅡC

  Spring, 理学研究科
• From Apr. 2011, To Mar. 2012

  生物化学ゼミナールⅡD

  Fall, 理学研究科
• From Apr. 2011, To Mar. 2012

  生物化学演習ⅡA

  Spring, 理学研究科
• From Apr. 2011, To Mar. 2012

  生物化学演習ⅡB

  Fall, 理学研究科
• From Apr. 2011, To Mar. 2012

  生物化学演習ⅡC

  Spring, 理学研究科
• From Apr. 2011, To Mar. 2012

  生物化学演習ⅡD

  Fall, 理学研究科
• 生物化学III
• ウイルスと生命科学
• ポケットゼミ
• 生体分子動態学
• 生物化学演習
• From Apr. 2011, To Mar. 2012

  The Front Line of Life Science Research II

  Spring, 全学共通科目
• From Apr. 2012, To Mar. 2013

  Virus and Life Science

  Fall, 全学共通科目
• From Apr. 2012, To Mar. 2013

  The Front Line of Life Science Research II

  Spring, 全学共通科目
• From Apr. 2012, To Mar. 2013

  Biochemistry III

  Fall, 理学部
• From Apr. 2012, To Mar. 2013

  Exercises in Biochemistry

  Fall, 理学部
• From Apr. 2013, To Mar. 2014

  Exercises in Biochemistry

  Fall, 理学部
• From Apr. 2013, To Mar. 2014

  Biochemistry III

  Fall, 理学部
• From Apr. 2013, To Mar. 2014

  Virus and Life Science

  Fall, 全学共通科目
• From Apr. 2013, To Mar. 2014

  The Front Line of Life Science Research II

  Spring, 全学共通科目
• From Apr. 2014, To Mar. 2015

  Exercises in Biochemistry

  Fall, 理学部
• From Apr. 2014, To Mar. 2015

  Biochemistry III

  Fall, 理学部
• From Apr. 2014, To Mar. 2015

  Dynamic aspects of proteins in the cell

  Fall, 理学研究科
• From Apr. 2014, To Mar. 2015

  Seminar on Biochemistry IIA

  Spring, 理学研究科
• From Apr. 2014, To Mar. 2015

  Seminar on Biochemistry IIB

  Fall, 理学研究科
• From Apr. 2014, To Mar. 2015

  Seminar on Biochemistry IIC

  Spring, 理学研究科
• From Apr. 2014, To Mar. 2015

  Seminar on Biochemistry IID

  Fall, 理学研究科
• From Apr. 2014, To Mar. 2015

  Advanced Biochemistry IIA

  Spring, 理学研究科
• From Apr. 2014, To Mar. 2015

  Advanced Biochemistry IIB

  Fall, 理学研究科
• From Apr. 2014, To Mar. 2015

  Advanced Biochemistry IIC

  Spring, 理学研究科
• From Apr. 2014, To Mar. 2015

  Advanced Biochemistry IID

  Fall, 理学研究科
• From Apr. 2014, To Mar. 2015

  Virus and Life Science

  Fall, 全学共通科目
• From Apr. 2015, To Mar. 2016

  Virus and Life Science

  Fall, 全学共通科目
• From Apr. 2015, To Mar. 2016

  Biochemistry III

  Fall, 理学部
• From Apr. 2015, To Mar. 2016

  Seminar on Biochemistry IIA

  Spring, 理学研究科
• From Apr. 2015, To Mar. 2016

  Seminar on Biochemistry IIB

  Fall, 理学研究科
• From Apr. 2015, To Mar. 2016

  Seminar on Biochemistry IIC

  Spring, 理学研究科
• From Apr. 2015, To Mar. 2016

  Seminar on Biochemistry IID

  Fall, 理学研究科
• From Apr. 2015, To Mar. 2016

  Advanced Biochemistry IIA

  Spring, 理学研究科
• From Apr. 2015, To Mar. 2016

  Advanced Biochemistry IIB

  Fall, 理学研究科
• From Apr. 2015, To Mar. 2016

  Advanced Biochemistry IIC

  Spring, 理学研究科
• From Apr. 2015, To Mar. 2016

  Advanced Biochemistry IID

  Fall, 理学研究科
• From Apr. 2015, To Mar. 2016

  Exercises in Biochemistry

  Fall, 理学部
• From Apr. 2016, To Mar. 2017

  Dynamic aspects of proteins in the cell

  Fall, 理学研究科
• From Apr. 2016, To Mar. 2017

  Biochemistry III

  Fall, 理学部
• From Apr. 2016, To Mar. 2017

  Seminar on Biochemistry IIA

  Spring, 理学研究科
• From Apr. 2016, To Mar. 2017

  Seminar on Biochemistry IIB

  Fall, 理学研究科
• From Apr. 2016, To Mar. 2017

  Seminar on Biochemistry IIC

  Spring, 理学研究科
• From Apr. 2016, To Mar. 2017

  Seminar on Biochemistry IID

  Fall, 理学研究科
• From Apr. 2016, To Mar. 2017

  Advanced Biochemistry IIA

  Spring, 理学研究科
• From Apr. 2016, To Mar. 2017

  Advanced Biochemistry IIB

  Fall, 理学研究科
• From Apr. 2016, To Mar. 2017

  Advanced Biochemistry IIC

  Spring, 理学研究科
• From Apr. 2016, To Mar. 2017

  Advanced Biochemistry IID

  Fall, 理学研究科
• From Apr. 2016, To Mar. 2017

  Exercises in Biochemistry

  Fall, 理学部
• From Apr. 2017, To Mar. 2018

  Biochemistry III

  Fall, 理学部
• From Apr. 2017, To Mar. 2018

  Seminar on Biochemistry IIA

  Spring, 理学研究科
• From Apr. 2017, To Mar. 2018

  Seminar on Biochemistry IIB

  Fall, 理学研究科
• From Apr. 2017, To Mar. 2018

  Seminar on Biochemistry IIC

  Spring, 理学研究科
• From Apr. 2017, To Mar. 2018

  Seminar on Biochemistry IID

  Fall, 理学研究科
• From Apr. 2017, To Mar. 2018

  Advanced Biochemistry IIA

  Spring, 理学研究科
• From Apr. 2017, To Mar. 2018

  Advanced Biochemistry IIB

  Fall, 理学研究科
• From Apr. 2017, To Mar. 2018

  Advanced Biochemistry IIC

  Spring, 理学研究科
• From Apr. 2017, To Mar. 2018

  Advanced Biochemistry IID

  Fall, 理学研究科
• From Apr. 2017, To Mar. 2018

  Exercises in Biochemistry

  Fall, 理学部
• From Apr. 2018, To Mar. 2019

  Dynamic aspects of proteins in the cell

  Fall, 理学研究科
• From Apr. 2018, To Mar. 2019

  Biochemistry III

  Fall, 理学部
• From Apr. 2018, To Mar. 2019

  Seminar on Biochemistry IIA

  Spring, 理学研究科
• From Apr. 2018, To Mar. 2019

  Seminar on Biochemistry IIB

  Fall, 理学研究科
• From Apr. 2018, To Mar. 2019

  Seminar on Biochemistry IIC

  Spring, 理学研究科
• From Apr. 2018, To Mar. 2019

  Seminar on Biochemistry IID

  Fall, 理学研究科
• From Apr. 2018, To Mar. 2019

  Advanced Biochemistry IIA

  Spring, 理学研究科
• From Apr. 2018, To Mar. 2019

  Advanced Biochemistry IIB

  Fall, 理学研究科
• From Apr. 2018, To Mar. 2019

  Advanced Biochemistry IIC

  Spring, 理学研究科
• From Apr. 2018, To Mar. 2019

  Advanced Biochemistry IID

  Fall, 理学研究科
• From Apr. 2018, To Mar. 2019

  Exercises in Biochemistry

  Fall, 理学部
• From Apr. 2019, To Mar. 2020

  Biochemistry III

  Fall, 理学部
• From Apr. 2019, To Mar. 2020

  Seminar on Biochemistry IIA

  Spring, 理学研究科
• From Apr. 2019, To Mar. 2020

  Seminar on Biochemistry IIB

  Fall, 理学研究科
• From Apr. 2019, To Mar. 2020

  Seminar on Biochemistry IIC

  Spring, 理学研究科
• From Apr. 2019, To Mar. 2020

  Seminar on Biochemistry IID

  Fall, 理学研究科
• From Apr. 2019, To Mar. 2020

  Advanced Biochemistry IIA

  Spring, 理学研究科
• From Apr. 2019, To Mar. 2020

  Advanced Biochemistry IIB

  Fall, 理学研究科
• From Apr. 2019, To Mar. 2020

  Advanced Biochemistry IIC

  Spring, 理学研究科
• From Apr. 2019, To Mar. 2020

  Advanced Biochemistry IID

  Fall, 理学研究科
• From Apr. 2019, To Mar. 2020

  Exercises in Biochemistry

  Fall, 理学部
• From Apr. 2020, To Mar. 2021

  Dynamic aspects of proteins in the cell

  Fall, 理学研究科
• From Apr. 2020, To Mar. 2021

  Biochemistry III

  Fall, 理学部
• From Apr. 2020, To Mar. 2021

  Seminar on Biochemistry IIA

  Spring, 理学研究科
• From Apr. 2020, To Mar. 2021

  Seminar on Biochemistry IIB

  Fall, 理学研究科
• From Apr. 2020, To Mar. 2021

  Seminar on Biochemistry IIC

  Spring, 理学研究科
• From Apr. 2020, To Mar. 2021

  Seminar on Biochemistry IID

  Fall, 理学研究科
• From Apr. 2020, To Mar. 2021

  Advanced Biochemistry IIA

  Spring, 理学研究科
• From Apr. 2020, To Mar. 2021

  Advanced Biochemistry IIB

  Fall, 理学研究科
• From Apr. 2020, To Mar. 2021

  Advanced Biochemistry IIC

  Spring, 理学研究科
• From Apr. 2020, To Mar. 2021

  Advanced Biochemistry IID

  Fall, 理学研究科
• From Apr. 2020, To Mar. 2021

  Exercises in Biochemistry

  Fall, 理学部
• From Apr. 2021, To Mar. 2022

  Biochemistry III

  Fall, 理学部
• From Apr. 2021, To Mar. 2022

  Seminar on Biochemistry IIA

  Spring, 理学研究科
• From Apr. 2021, To Mar. 2022

  Seminar on Biochemistry IIB

  Fall, 理学研究科
• From Apr. 2021, To Mar. 2022

  Seminar on Biochemistry IIC

  Spring, 理学研究科
• From Apr. 2021, To Mar. 2022

  Seminar on Biochemistry IID

  Fall, 理学研究科
• From Apr. 2021, To Mar. 2022

  Advanced Biochemistry IIA

  Spring, 理学研究科
• From Apr. 2021, To Mar. 2022

  Advanced Biochemistry IIB

  Fall, 理学研究科
• From Apr. 2021, To Mar. 2022

  Advanced Biochemistry IIC

  Spring, 理学研究科
• From Apr. 2021, To Mar. 2022

  Advanced Biochemistry IID

  Fall, 理学研究科
• From Apr. 2021, To Mar. 2022

  Exercises in Biochemistry

  Fall, 理学部

Participation in PhD Defense

• DNA Nanotechnology and Atomic Level Understanding for a Complex of DNA and a DNA Minor Groove Binder

  ABE KATSUHIKO, Graduate School of Science, Sub-chief Examiner

  25 Mar. 2024
• Development of Cyclic and Hairpin Pyrrole-Imidazole Polyamides for Specific Recognition of Disease-Associated DNA Sequences

  HIROSE YUKI, Graduate School of Science, Sub-chief Examiner

  25 Mar. 2024
• The molecular associations in clathrin-coated pit regulate β-arrestin-mediated MAPK signaling downstream of μ-opioid receptor

  SATO ATSUKO, Graduate School of Medicine, Sub-chief Examiner

  23 Mar. 2023
• 大腸菌膜内切断プロテアーゼRsePが有する新奇生理機能の解明

  YOKOYAMA TATSUHIKO, Graduate School of Science, Chief Examiner

  23 Mar. 2023
• Investigation of FRET System and Fluorine-Containing Nucleic Acids by Artificial Nucleobases

  HIRASHIMA SHINGO, Graduate School of Science, Sub-chief Examiner

  23 Mar. 2023
• Guanylate-Binding Protein 1 Regulates Infection-Induced Autophagy through TBK1 Phosphorylation

  HIKICHI MIYAKO, Graduate School of Medicine, Sub-chief Examiner

  23 Jan. 2023
• Chemical biology studies on nucleic acid recognition, modification, and secondary structures

  JOSEPHBATH SAHAYA SHEELA VINODH, Graduate School of Science, Sub-chief Examiner

  25 Jul. 2022
• 大腸菌S2Pファミリー膜内切断プロテアーゼRsePの基質選別・認識機構

  MIYAKE TAKUYA, Graduate School of Science, Chief Examiner

  23 May 2022
• Investigating Advanced Properties of Modular DNAs as Biohybrid Catalyst and Aptamer

  Yum Ji Hye, Graduate School of Science, Sub-chief Examiner

  23 Mar. 2022
• Biomolecular nanotechnology-based approaches to investigate nucleic acid interactions

  SHUBHAM MISHRA, Graduate School of Science, Sub-chief Examiner

  23 Mar. 2022
• DNA based Photo-controllable Extracellular Matrix-like Scaffolds to Understand and Control Cell Behaviour

  SOUMYA SETHI, Graduate School of Science, Sub-chief Examiner

  23 Mar. 2022
• FBXO2/SCF ubiquitin ligase complex directs xenophagy through recognizing bacterial surface glycan

  YAMADA AKIHIRO, Graduate School of Medicine, Sub-chief Examiner

  24 Jan. 2022
• Dynamic Meso-Scale Anchorage of GPI-Anchored Receptors in the Plasma Membrane: Prion Protein vs. Thy1

  NEMOTO YURI, Graduate School of Medicine, Sub-chief Examiner

  23 Mar. 2022
• Hydroxypropyl Cyclodextrin Improves Amiodarone-Induced Aberrant Lipid Homeostasis of Alveolar Cells

  KANAGAKI SHUHEI, Graduate School of Medicine, Sub-chief Examiner

  23 Mar. 2022

Student achievements: Awards

• 第16回21世紀大腸菌研究会　優秀ポスター発表賞

  Takuya Miyake, 大腸菌研究会, 39 May 2019
• 第16回21世紀大腸菌研究会　優秀ポスター発表賞

  Tatsuhiko Yokoyama, 大腸菌研究会　, 30 May 2019
• 第92回日本生化学会大会　若手優秀発表賞

  Tatsuhiko Yokoyama, 日本生化学会, 19 Sep. 2019
• 第93回日本生化学会大会　若手優秀発表賞

  Tatsuhiko Yokoyama, 日本生化学会, 16 Sep. 2020
• 第17回21世紀大腸菌研究会　口頭発表賞

  Tatsuhiko Yokoyama, 21世紀大腸菌研究会, 20 Aug. 2021
• 第18回21世紀大腸菌研究会　研究優秀賞

  Tatsuhiko Yokoyama, 21世紀大腸菌研究会, 28 Jun. 2022

Student achievements: Papers

• Edge strand of Escherichia coli BepA interacts with immature LptD on the β-barrel assembly machine to direct it to on- and off-pathways.

  2021, Miyazaki, R., Watanabe, T., Yoshitani, K., and Akiyama, Y., eLife, 10, e70541, Peer-reviewed, English
• An in vivo protease activity assay for investigating the functions of the Escherichia coli membrane protease HtpX.

  Yoshitani, K., Hizukuri, Y., and Akiyama, Y.

  2019, FEBS Lett., 593, 843, 851, Peer-reviewed, English
• Involvement of a membrane-bound amphiphilic helix in substrate discrimination and binding by an Escherichia coli S2P peptidase RseP.

  Miyake, T., Hizukuri, Y., and Akiyama, Y.

  2020, Front. Microbiol., 11, 607381, Peer-reviewed, English
• Moving toward generalizable NZ-1 labeling for 3D structure determination with optimized epitope tag insertion.

  Tamura-Sakaguchi, R., Aruga, R., Hirose, M., Ekimoto, T., Miyake, T., Hizukuri, Y., Oi, R., Kaneko, M. M., Kato, Y., Akiyama, Y., Ikeguchi, M., Iwasaki, K., and Nogi, T.

  2021, Acta Crystallogr. D, 77, 645, 662, Peer-reviewed, English, Scientific journal, False

  https://doi.org/https://doi.org/10.1101/2020.10.06.325969
• The Escherichia coli S2P intramembrane protease RseP regulates ferric citrate uptake by cleaving the sigma factor regulator FecR.

  Yokoyama, T., Niinae, T., Tsumagari, K., Imami, K., Ishihama, Y., Hizukuri, Y., and Akiyama, Y.

  2021, J. Biol. Chem., 296, 100637, 100637, Peer-reviewed, English, Scientific journal, False

  https://doi.org/10.1016/j.jbc.2021.100673
• Cryo-EM structure of an entire AAA protease complex FtsH-HflKC.

  Qiao, Z., Yokoyama, T., Yan, X.-F., Beh, I. T., Shi, J., Basek, S., Akiyama, Y., and Gao, Y.-G.

  2022, Cell reports, 39, 110890, 110890, Peer-reviewed, English, Scientific journal, True

  https://doi.org/https://doi.org/10.1016/j.celrep.2022.110890

Student achievements: Presentations

• 大腸菌膜内切断プロテアーゼRsePの新規基質切断とその生理的意義: 低分子膜タンパク質HokBの切断を介したパーシスター形成制御

  13. 横山達彦

  第92回日本生化学会大会, 19 Sep. 2019, From 18 Sep. 2019, To 20 Sep. 2019, English, Poster presentation
• 大腸菌膜内切断プロテアーゼRsePの基質切断と構造維持に重要な膜表在・両親媒性ヘリックスの解析

  三宅拓也

  第92回日本生化学会大会, 19 Sep. 2019, From 18 Sep. 2019, English, Poster presentation
• 部位特異的in vivo光架橋法による大腸菌LptDの生合成過程の解析

  9. 渡邊哲朗

  第16回21世紀大腸菌研究会, 30 May 2019, From 29 May 2019, To 30 May 2019, English, Oral presentation
• 膜内切断プロテアーゼRsePの新奇切断基質とその切断の生理的意義〜toxinタンパク質HokBの切断を介したpersister状態制御〜

  8. 横山達彦

  第16回21世紀大腸菌研究会, 30 May 2019, From 29 May 2019, To 30 May 2019, English, Oral presentation
• 大腸菌膜内切断プロテアーゼRsePの基質切断と構造維持に重要な膜表在・両親媒性ヘリックスPCT-H1の解析

  7. 三宅拓也

  第16回21世紀大腸菌研究会, 29 May 2019, From 29 May 2019, To 30 May 2019, English, Oral presentation
• 部位特異的 in vivo 光架橋法を用いた大腸菌のLPSトランスロコンサブユニットLptDの生合成過程の解析

  4. 渡邉哲朗

  日本農芸化学会2019年度大会, 25 Mar. 2019, From Mar. 2019, To 27 Mar. 2019, English, Poster presentation
• 大腸菌S2PファミリープロテアーゼRsePの基質探索：鉄取り込みに関与する転写制御因子の切断とその生理的意義

  4. 横山達彦

  第93回日本生化学会大会, 15 Sep. 2020, From 14 Sep. 2020, To 16 Sep. 2020, Japanese, Oral presentation
• The Escherichia coli intramembrane protease RseP regulates iron uptake by cleaving the sigma factor regulator FecR.

  Tatsuhiko Yokoyama

  The 27th East Asia Joint Symposium, 28 Oct. 2021, From 27 Oct. 2021, To 29 Oct. 2021, English, Oral presentation
• 部位特異的in vivo光架橋法によるタンパク質膜透過促進因子SecD/Fと膜結合型分子シャペロンPpiD/YfgM複合体の相互作用解析

  Ai Mengting

  第17回21世紀大腸菌研究会, 20 Aug. 2021, From 20 Aug. 2021, To 20 Aug. 2021, Japanese, Oral presentation
• 大腸菌膜内切断プロテアーゼRsePはFecRを膜内で切断し、鉄取り込みに関わる遺伝子群の転写を制御する

  Tatsuhiko Yokoyama

  第17回21世紀大腸菌研究会, 20 Aug. 2021, From 20 Aug. 2021, To 20 Aug. 2021, Japanese, Oral presentation
• 大腸菌膜内切断プロテアーゼRsePの新奇生理機能：FecRの切断を介した鉄取り込みに関わる遺伝子群の転写活性化

  Tatsuhiko Yokoyama

  日本農芸化学会2021年度大会, 20 Mar. 2021, From 18 Mar. 2021, To 21 Mar. 2021, Not invited, Japanese, Oral presentation

Administration

School management (title, position)

• From 01 Apr. 2022, To 31 Mar. 2023

  教育制度委員会 委員
• From 01 Jul. 2009, To 31 Mar. 2011

  英文一覧等小委員会 委員
• From 01 Jul. 2009, To 31 Mar. 2011

  国際交流委員会 委員
• From 01 Apr. 2008, To 31 Mar. 2010

  吉田キャンパス整備専門委員会委員会 委員
• From 01 Apr. 2008, To 31 Mar. 2011

  放射線障害予防小委員会 委員
• From 01 Apr. 2009, To 31 Mar. 2011

  放射性同位元素等管理委員会 委員
• From 01 Apr. 2010

  大学評価委員会　点検・評価実行委員会　委員
• From 01 Apr. 2011, To 31 Mar. 2013

  放射性同位元素等専門委員会　委員
• From 01 Apr. 2011, To 31 Mar. 2012

  放射性障害予防小委員会　委員
• From 01 Apr. 2011, To 31 Mar. 2012

  情報セキュリティ連絡責任者
• From 01 Apr. 2010

  ウイルス研究所放射線取扱主任者
• From 01 Apr. 2011, To 31 Mar. 2013

  国際交流委員会 委員
• From 01 Apr. 2011, To 31 Mar. 2012

  大学評価委員会点検・評価実行委員会 委員
• From 01 Apr. 2012, To 31 Mar. 2013

  大学評価委員会 点検・評価実行委員会 委員
• From 01 Jul. 2009, To 30 Jun. 2011

  国際交流委員会 委員

Faculty management (title, position)

• From 01 Apr. 2024, To 31 Mar. 2025

  放射線管理責任者
• From 01 Apr. 2024, To 31 Mar. 2025

  放射線取扱主任者
• From 01 Apr. 2022, To 31 Mar. 2026

  環境安全保健機構附属放射性同位元素総合センター共同利用検討委員会　委員
• From 01 Apr. 2022

  業績評価委員会
• From 01 Apr. 2022, To 31 Mar. 2024

  予算委員会（医生物学研究所）
• From 01 Apr. 2014

  人権委員会　委員
• From 01 Apr. 2018

  自己点検・評価委員会　委員
• From 01 Apr. 2020

  放射線管理委員会　委員
• From 01 Jun. 2017, To 31 Mar. 2024

  施設整備委員会　委員
• From 01 Apr. 2018, To 05 Jan. 2024

  研究データ保全委員会
• From 01 Apr. 2010, To 31 Mar. 2012

  予算委員会　委員
• From 01 Apr. 2011, To 31 Mar. 2013

  部屋割検討委員会　委員
• From 01 Apr. 2010, To 31 Mar. 2012

  情報セキュリティ委員会　委員
• From 27 Sep. 1993, To 31 Mar. 1995

  コンピュータ・ネットワーク委員会　委員
• From 01 Apr. 2010, To 31 Mar. 2012

  共同利用委員会　委員
• 防火対策委員会　委員
• From 01 Apr. 2014, To 31 Mar. 2017

  職員等配置検討委員会　委員長
• From 01 Apr. 2013, To 31 Mar. 2017

  部屋割検討委員会　委員
• From 01 Apr. 2014

  自己点検・評価委員　委員長
• From 01 Apr. 2014

  病原体安全管理委員会　委員
• From 01 Apr. 2014, To 30 Sep. 2016

  放射線障害防止委員会　委員長
• From 01 Apr. 2014

  ヒトレトロウイルス研究施設業務管理委員会　委員
• From 01 Apr. 2024

  安全衛生委員会　委員
• To 31 Mar. 2017

  コンピュータ・ネットワーク委員会　委員長
• From 01 Oct. 2016, To 31 Mar. 2020

  放射線障害防止委員会　委員
• From 01 Oct. 2016

  ウイルス感染症・生命科学先端融合的共同研究拠点専門委員会　委員
• From 01 Apr. 2011, To 31 Mar. 2013

  自己点検・評価委員会　委員
• From 01 Apr. 2020, To 31 Mar. 2021

  （旧ウイルス研）予算委員会
• From 06 May 2021, To 31 Mar. 2022

  ウイルス・再生医科学研究所予算委員