Basic Information

Email Address

• Email Address

  hayashi.gosuke.7skyoto-u.ac.jp

Professional Memberships

• 日本ペプチド学会
• 日本化学会（生体機能関連化学部会）
• 日本ケミカルバイオロジー学会
• 日本エピジェネティクス研究会
• 日本生化学会
• 日本分子生物学会

Academic Degree

• 24 Mar. 2009

  大阪大学博士（理学）
• 23 Mar. 2006

  京都大学修士（工学）

Academic Resume (Undergraduate School/Majors)

• 京都大学, 工学部工業化学科, 卒業

Research History

• From Oct. 2026, To Present

  Kyoto University, Institute for Life and Medical Science, 教授
• From 01 Mar. 2019, To Present

  Nagoya University, Graduate School of Engineering Biomolecular Engineering 1, Associate professor
• From Feb. 2013, To Feb. 2019

  The University of Tokyo, Graduate School of Engineering, Department of Chemistry and Biotechnology, Assistant Professor
• From Jun. 2012, To Jan. 2013

  The University of Tokyo, Research Center for Advanced Science and Technology, Designated assistant professor
• From Feb. 2011, To May 2012

  Boston University, Department of Biomedical Engineering, Postdoctral Fellow (JSPS Reserch Fellowship for Young Scientists PD and Overseas Research Fellowships)
• From Apr. 2009, To Feb. 2011

  The University of Tokyo, Research Center for Advanced Science and Technology, Postdoctral Fellow (JSPS Research Fellowship for Young Scientists PD)
• From Apr. 2006, To Mar. 2009

  Osaka University, Graduate School of Science, Department of Chemistry, JSPS Research Fellowship of Young Scientists DC1

ID,URL

• ORCID ID

  0000-0001-6853-2706

• J-Global ID

  201901013256543966

• Researcher number

  40648268

researchmap URL

• https://researchmap.jp/ghayashi

Research

Research Interests

• Epigenetics
• Peptide chemistry
• Chemical protein synthesis

Research Areas

• Nanotechnology/Materials, Biochemistry
• Nanotechnology/Materials, Molecular biochemistry

Papers

• Improvement of cDNA TRAP Display via Optimization of Puromycin Linker Design for Enhanced Discovery of Antibody‐Like Proteins

  Haruto Kosugi; Hiroki Nakanishi; Gosuke Hayashi; Hiroshi Murakami

  ChemBioChem, 26 May 2026

  
• De novo chemo-optogenetics through the rational design of photoresponsive molecules and selection of their artificial protein binding pairs

  Tomoki Miyazaki; Tomoshige Fujino; Tatsuyuki Yoshii; Haruto Kosugi; Mamoru Funane; Naoya Murata; Kim Chung Nguyen; Satoru Nagatoishi; Kouhei Tsumoto; Gosuke Hayashi; Hiroshi Murakami; Shinya Tsukiji

  Nature Chemistry, 23 Apr. 2026

  
• Chemical Protein Synthesis via Direction-Switching One-Pot Peptide Ligation Enabled by Orthogonal Cysteinyl Protection.

  Koki Nakatsu; Fumika Yoshitomi; Hiroki Onoda; Reia Shikimachi; Kyohei Arita; Akimitsu Okamoto; Hiroshi Murakami; Gosuke Hayashi

  Journal of the American Chemical Society, 04 Feb. 2026

  
• Design and Evaluation of Stable Cysteine-Modified Monobody Scaffolds for Mirror-Image Synthesis.

  Naoya Iwamoto; Saya Ohno; Kensuke Nakamura; Toshinori Naito; Sayaka Miura; Shinsuke Inuki; Hiroaki Ohno; Gosuke Hayashi; Hiroshi Murakami; Shinya Oishi

  Bioconjugate chemistry, 16 Jul. 2025

  
• De novochemo-optogenetics through rational small-molecule design and TRAP display

  Tomoki Miyazaki; Tomoshige Fujino; Tatsuyuki Yoshii; Mamoru Funane; Naoya Murata; Chung Nguyen Kim; Satoru Nagatoishi; Kouhei Tsumoto; Gosuke Hayashi; Hiroshi Murakami; Shinya Tsukiji

  10 Apr. 2025

  
• Generating a mirror-image monobody targeting MCP-1 via TRAP display and chemical protein synthesis

  Hayashi, Gosuke; Naito, Toshinori; Miura, Sayaka; Iwamoto, Naoya; Usui, Yusuke; Bando-Shimizu, Mika; Suzuki, Sae; Higashi, Katsuaki; Nonaka, Motohiro; Oishi, Shinya; Murakami, Hiroshi

  Nature communications, 23 Dec. 2024

  
• A tRNA modification with aminovaleramide facilitates AUA decoding in protein synthesis

  Kenjyo Miyauchi; Satoshi Kimura; Naho Akiyama; Kazuki Inoue; Kensuke Ishiguro; Thien-Son Vu; Veerasak Srisuknimit; Kenta Koyama; Gosuke Hayashi; Akiko Soma; Asuteka Nagao; Mikako Shirouzu; Akimitsu Okamoto; Matthew K. Waldor; Tsutomu Suzuki

  Nature Chemical Biology, 19 Sep. 2024

  
• Nucleic acid scaffold-assisted peptide ligation for chemical protein synthesis

  Hayashi, G.

  JOURNAL OF PEPTIDE SCIENCE, Aug. 2024

  
• Fmoc solid-phase synthesis of c-terminal diketopiperazine thioester peptides

  Nakajima, Y.; Nakatsu, K.; Hayashi, G.; Murakami, H.

  JOURNAL OF PEPTIDE SCIENCE, Aug. 2024

  
• Chemical protein synthesis via PNA scaffold-assisted peptide ligation

  Suzuki, S.; Mizushima, S.; Takeuchi, Y.; Hayashi, G.; Murakami, H.

  JOURNAL OF PEPTIDE SCIENCE, Aug. 2024

  
• H3K4me1 recruits DNA repair proteins in plants

  Quiroz, Daniela; Oya, Satoyo; Lopez-Mateos, Diego; Zhao, Kehan; Pierce, Alice; Ortega, Lissandro; Ali, Alissza; Carbonell-Bejerano, Pablo; Yarov-Yarovoy, Vladimir; Suzuki, Sae; Hayashi, Gosuke; Osakabe, Akihisa; Monroe, Grey

  The Plant cell, 29 May 2024

  
• Optineurin provides a mitophagy contact site for TBK1 activation

  Yamano, Koji; Sawada, Momoha; Kikuchi, Reika; Nagataki, Kafu; Kojima, Waka; Endo, Ryu; Kinefuchi, Hiroki; Sugihara, Atsushi; Fujino, Tomoshige; Watanabe, Aiko; Tanaka, Keiji; Hayashi, Gosuke; Murakami, Hiroshi; Matsuda, Noriyuki

  The EMBO journal, 01 Mar. 2024

  
• High-affinity mirror-image monobody targeting MCP-1 generated via TRAP display and chemical protein synthesis

  Murakami, Hiroshi; Hayashi, Gosuke; Naito, Toshinori; Miura, Sayaka; Iwamoto, Naoya; Usui, Yusuke; Nonaka, Motohiro; Oishi, Shinya

  Research Square, 2024

  
• One-pot ligation of multiple peptide segments via N-terminal thiazolidine deprotection chemistry

  Nakamura, Genki; Nakatsu, Koki; Hayashi, Gosuke

  Methods in enzymology, 2024

  
• Contributions of histone tail clipping and acetylation in nucleosome transcription by RNA polymerase II

  Oishi, Takumi; Hatazawa, Suguru; Kujirai, Tomoya; Kato, Junko; Kobayashi, Yuki; Ogasawara, Mitsuo; Akatsu, Munetaka; Ehara, Haruhiko; Sekine, Shun-Ichi; Hayashi, Gosuke; Takizawa, Yoshimasa; Kurumizaka, Hitoshi

  Nucleic acids research, 27 Oct. 2023

  
• Large-scale analysis of mRNA sequences localized near the start and amber codons and their impact on the diversity of mRNA display libraries

  Umemoto, Shun; Kondo, Taishi; Fujino, Tomoshige; Hayashi, Gosuke; Murakami, Hiroshi

  Nucleic acids research, 11 Aug. 2023

  
• Thiocholine-Mediated One-Pot Peptide Ligation and Desulfurization.

  Sae Suzuki; Yuya Nakajima; Naoki Kamo; Akihisa Osakabe; Akimitsu Okamoto; Gosuke Hayashi; Hiroshi Murakami

  Molecules (Basel, Switzerland), 22 Apr. 2023

  
• cIAP1-based degraders induce degradation via branched ubiquitin architectures

  Akizuki, Yoshino; Morita, Mai; Mori, Yuki; Kaiho-Soma, Ai; Dixit, Shivani; Endo, Akinori; Shimogawa, Marie; Hayashi, Gosuke; Naito, Mikihiko; Okamoto, Akimitsu; Tanaka, Keiji; Saeki, Yasushi; Ohtake, Fumiaki

  Nature chemical biology, 2023

  
• Structural basis for the unique multifaceted interaction of DPPA3 with the UHRF1 PHD finger

  Hata, Keiichi; Kobayashi, Naohiro; Sugimura, Keita; Qin, Weihua; Haxholli, Deis; Chiba, Yoshie; Yoshimi, Sae; Hayashi, Gosuke; Onoda, Hiroki; Ikegami, Takahisa; Mulholland, Christopher B; Nishiyama, Atsuya; Nakanishi, Makoto; Leonhardt, Heinrich; Konuma, Tsuyoshi; Arita, Kyohei

  Nucleic acids research, 28 Nov. 2022

  
• Repetitive Thiazolidine Deprotection Using a Thioester-Compatible Aldehyde Scavenger for One-Pot Multiple Peptide Ligation.

  Koki Nakatsu; Akimitsu Okamoto; Gosuke Hayashi; Hiroshi Murakami

  Angewandte Chemie (International ed. in English), 26 Sep. 2022

  
• Peptide selenoester synthesis via diketopiperazine formation

  Hayashi, G.; Hashimoto, M.; Nakatsu, K.; Murakami, H.

  JOURNAL OF PEPTIDE SCIENCE, Aug. 2022

  
• One-pot peptide ligation mediated by 2-aminobenzamide-type aldehyde scavengers

  Nakatsu, K.; Okamoto, A.; Murakami, H.; Hayashi, G.

  JOURNAL OF PEPTIDE SCIENCE, Aug. 2022

  
• Monobodies with potent neutralizing activity against SARS-CoV-2 Delta and other variants of concern

  Kondo, Taishi; Matsuoka, Kazuhiro; Umemoto, Shun; Fujino, Tomoshige; Hayashi, Gosuke; Iwatani, Yasumasa; Murakami, Hiroshi

  Life science alliance, Jun. 2022

  
• Construction of a Highly Diverse mRNA Library for in vitro Selection of Monobodies

  Kondo, Taishi; Eguchi, Minori; Tsuzuki, Nariaki; Murata, Naoya; Fujino, Tomoshige; Hayashi, Gosuke; Murakami, Hiroshi

  Bio-protocol, 20 Aug. 2021

  
• Base-resolution analysis of 5-hydroxymethylcytidine by selective oxidation and reverse transcription arrest.

  Kenta Koyama; Gosuke Hayashi; Hiroki Ueda; Satoshi Ota; Genta Nagae; Hiroyuki Aburatani; Akimitsu Okamoto

  Organic & biomolecular chemistry, 28 Jul. 2021

  
• Silyl-protected propargyl glycine for multiple labeling of peptides by chemoselective silyl-deprotection

  Naoki Kamo; Gosuke Hayashi; Akimitsu Okamoto

  Tetrahedron Letters, 08 Jun. 2021

  
• cDNA TRAP display for rapid and stable in vitro selection of antibody-like proteins.

  Taishi Kondo; Minori Eguchi; Seita Kito; Tomoshige Fujino; Gosuke Hayashi; Hiroshi Murakami

  Chemical communications (Cambridge, England), 07 Mar. 2021

  
• Thiazolidine Deprotection by 2-Aminobenzamide-Based Aldehyde Scavenger for One-Pot Multiple Peptide Ligation

  Nakatsu, Koki; Murakami, Hitoshi; Hayashi, Gosuke; Okamoto, Akimitsu

  ChemRxiv, 2021

  
• Organoruthenium-catalyzed chemical protein synthesis to elucidate the functions of epigenetic modifications on heterochromatin factors

  Naoki Kamo; Tomoya Kujirai; Hitoshi Kurumizaka; Hiroshi Murakami; Gosuke Hayashi; Akimitsu Okamoto

  Chemical Science, 2021

  
• Acetylation-modulated communication between the H3 N-terminal tail domain and the intrinsically disordered H1 C-terminal domain

  Hao, Fanfan; Murphy, Kevin J; Kujirai, Tomoya; Kamo, Naoki; Kato, Junko; Koyama, Masako; Okamato, Akimitsu; Hayashi, Gosuke; Kurumizaka, Hitoshi; Hayes, Jeffrey J

  Nucleic acids research, 18 Nov. 2020

  
• L-DNA-tagged fluorescence: In situ hybridization for highly sensitive imaging of RNAs in single cells

  Motoyuki Ogata; Gosuke Hayashi; Anri Ichiu; Akimitsu Okamoto

  Organic and Biomolecular Chemistry, 28 Oct. 2020

  
• Antibody-like proteins that capture and neutralize SARS-CoV-2

  Kondo, T.; Iwatani, Y.; Matsuoka, K.; Fujino, T.; Umemoto, S.; Yokomaku, Y.; Ishizaki, K.; Kito, S.; Sezaki, T.; Hayashi, G.; Murakami, H.

  SCIENCE ADVANCES, Oct. 2020

  
• Toolbox for chemically synthesized histone proteins

  Nakatsu, Koki; Hayashi, Gosuke; Okamoto, Akimitsu

  Current opinion in chemical biology, Oct. 2020

  
• Fmoc-Compatible and C-terminal-Sequence-Independent Peptide Alkyl Thioester Formation Using Cysteinylprolyl Imide

  Nakatsu, Koki; Yanase, Masafumi; Hayashi, Gosuke; Okamoto, Akimitsu

  Organic letters, 19 Jun. 2020

  
• Novel Strategies of Peptide Ligation for Accelerating Chemical Protein Synthesis

  Hayashi Gosuke; Okamoto Akimitsu

  JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY JAPAN, Feb. 2020

  
• Fmoc-Compatible and External-Thiol-Free Peptide C-Terminus Alkyl Thioester Formation Using Cysteinylprolyl Imide

  Nakatsu, Koki; Yanase, Masafumi; Hayashi, Gosuke; Okamoto, Akimitsu

  ChemRxiv, 2020

  
• Ru Catalyst Facilitates Total Chemical Protein Synthesis to Investigate the Effect of Epigenetic Modifications Decorated on Linker Histone H1.2 and Heterochromatin Protein 1α (HP1α)

  Kamo, Naoki; Kujirai, Tomoya; Kurumizaka, Hitoshi; Murakami, Hitoshi; Hayashi, Gosuke; Okamoto, Akimitsu

  ChemRxiv, 2020

  
• タンパク質化学合成を活用した翻訳後修飾研究

  林 剛介

  ファルマシア, 2020

  
• Chemical Synthesis of Cys-Containing Protein via Chemoselective Deprotection with Different Palladium Complexes

  Kamo, Naoki; Hayashi, Gosuke; Okamoto, Akimitsu

  Organic letters, 18 Oct. 2019

  
• Cysteinylprolyl imide (CPI) peptide: a highly reactive and easily accessible crypto-thioester for chemical protein synthesis

  Yanase, Masafumi; Nakatsu, Koki; Cardos, Charlane Joy; Konda, Yoshiki; Hayashi, Gosuke; Okamoto, Akimitsu

  Chemical science, 21 Jun. 2019

  
• Reaction design for highly efficient chemical protein synthesis

  Hayashi Gosuke; Okamoto Akimitsu

  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 31 Mar. 2019

  
• Simultaneous and Traceless Ligation of Peptide Fragments on DNA Scaffold

  Hayashi, Gosuke; Yanase, Masafumi; Nakatsuka, Yu; Okamoto, Akimitsu

  Biomacromolecules, 11 Mar. 2019

  
• Corrigendum: Triple Function of 4-Mercaptophenylacetic Acid Promotes One-Pot Multiple Peptide Ligation

  Kamo, Naoki; Hayashi, Gosuke; Okamoto, Akimitsu

  Angewandte Chemie (International ed. in English), Feb. 2019, Peer-reviewed

  
• Triple Function of 4-Mercaptophenylacetic Acid Promotes One-Pot Multiple Peptide Ligation

  Kamo, Naoki; Hayashi, Gosuke; Okamoto, Akimitsu

  Angewandte Chemie (International ed. in English), 10 Dec. 2018

  
• Chemistry-Driven Epigenetic Investigation of Histone and DNA Modifications

  Sueoka, Takuma; Koyama, Kenta; Hayashi, Gosuke; Okamoto, Akimitsu

  Chemical record (New York, N.Y.), Dec. 2018

  
• Efficient peptide ligation between allyl-protected Asp and Cys followed by palladium-mediated deprotection

  Kamo, Naoki; Hayashi, Gosuke; Okamoto, Akimitsu

  Chemical communications (Cambridge, England), 24 Apr. 2018

  
• Hybridization-sensitive Fluorescent Oligonucleotide Probe Conjugated with Cell-penetrating Peptides for Enhanced Cellular Uptake

  Hayashi Gosuke; Tamai Makoto; Okamoto Akimitsu

  CHEMISTRY LETTERS, Dec. 2017

  
• Regulation of the Stability of the Histone H2A-H2B Dimer by H2A Tyr57 Phosphorylation

  Sueoka, Takuma; Hayashi, Gosuke; Okamoto, Akimitsu

  Biochemistry, 12 Sep. 2017

  
• Chemical synthesis of dual labeled proteins via differently protected alkynes enables intramolecular FRET analysis

  Hayashi, Gosuke; Kamo, Naoki; Okamoto, Akimitsu

  Chemical communications (Cambridge, England), 30 May 2017

  
• Chemically-activatable alkyne-tagged probe for imaging microdomains in lipid bilayer membranes

  Yamaguchi, Satoshi; Matsushita, Taku; Izuta, Shin; Katada, Sumika; Ura, Manami; Ikeda, Taro; Hayashi, Gosuke; Suzuki, Yuta; Kobayashi, Koya; Tokunaga, Kyoya; Ozeki, Yasuyuki; Okamoto, Akimitsu

  Scientific reports, 24 Jan. 2017

  
• Base-Resolution Analysis of 5-Hydroxymethylcytosine by One-Pot Bisulfite-Free Chemical Conversion with Peroxotungstate.

  Hayashi G; Koyama K; Shiota H; Kamio A; Umeda T; Nagae G; Aburatani H; Okamoto A

  Journal of the American Chemical Society, 02 Nov. 2016

  
• In vitro and in cell analysis of chemically synthesized histone H2A with multiple modifications

  Hayashi, Gosuke; Sueoka, Takuma; Okamoto, Akimitsu

  Chemical communications (Cambridge, England), 11 Apr. 2016

  
• Diazirine Photocrosslinking Recruits Activated FTO Demethylase Complexes for Specific N-6-methyladenosine Recognition

  Hyun Seok Jeong; Gosuke Hayashi; Akimitsu Okamoto

  ACS CHEMICAL BIOLOGY, Jun. 2015, Peer-reviewed

  
• 2-Oxazoline formation for selective chemical labeling of 5-hydroxylysine

  Hayashi, Gosuke; Sakamoto, Ryosuke; Okamoto, Akimitsu

  Chemistry, an Asian journal, May 2015

  
• Hybridization-sensitive fluorescent oligonucleotide probe conjugated with a bulky module for compartment-specific mRNA monitoring in a living cell

  Hayashi, Gosuke; Yanase, Masafumi; Takeda, Katsuya; Sakakibara, Daisuke; Sakamoto, Ryosuke; Wang, Dan Ohtan; Okamoto, Akimitsu

  Bioconjugate chemistry, 18 Mar. 2015

  
• Middle-Down and Chemical Proteomic Approaches to Reveal Histone H4 Modification Dynamics in Cell Cycle: Label-Free Semi-Quantification of Histone Tail Peptide Modifications Including Phosphorylation and Highly Sensitive Capture of Histone PTM Binding Proteins Using Photo-Reactive Crosslinkers.

  Yamamoto K; Chikaoka Y; Hayashi G; Sakamoto R; Yamamoto R; Sugiyama A; Kodama T; Okamoto A; Kawamura T

  Mass spectrometry (Tokyo, Japan), 2015

  
• An orthogonal ribosome-tRNA pair via engineering of the peptidyl transferase center.

  Terasaka N; Hayashi G; Katoh T; Suga H

  Nature chemical biology, Jul. 2014

  
• Development of photoswitchable RNA aptamer-ligand complexes

  Hayashi, Gosuke; Nakatani, Kazuhiko

  Methods in molecular biology (Clifton, N.J.), 2014

  
• Probe design for the effective fluorescence imaging of intracellular RNA

  Hayashi, Gosuke; Okamoto, Akimitsu

  Chemical record (New York, N.Y.), Apr. 2013

  
• A nucleic acid probe labeled with desmethyl thiazole orange: a new type of hybridization-sensitive fluorescent oligonucleotide for live-cell RNA imaging

  Okamoto, Akimitsu; Sugizaki, Kaori; Yuki, Mizue; Yanagisawa, Hiroyuki; Ikeda, Shuji; Sueoka, Takuma; Hayashi, Gosuke; Wang, Dan Ohtan

  Organic & biomolecular chemistry, 14 Jan. 2013

  
• Activation of prokaryotic translation by antisense oligonucleotides binding to coding region of mRNA

  Hayashi, Gosuke; Hong, Changfeng; Hagihara, Masaki; Nakatani, Kazuhiko

  Biochemical and biophysical research communications, 07 Dec. 2012

  
• The RNA origin of transfer RNA aminoacylation and beyond

  Suga, Hiroaki; Hayashi, Gosuke; Terasaka, Naohiro

  Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 27 Oct. 2011

  
• [Ribosomal synthesis of nonstandard cyclic peptides and its application to drug discovery]

  Hayashi, Gosuke; Ohshiro, Yukinori; Suga, Hiroaki

  Seikagaku. The Journal of Japanese Biochemical Society, Jun. 2010

  
• Ribosome evolution for two artificial amino acids in E. coli.

  Hayashi G; Goto Y; Suga H

  Chemistry & biology, 23 Apr. 2010

  
• RNA aptamers that reversibly bind photoresponsive azobenzene-containing peptides

  Hayashi, Gosuke; Hagihara, Masaki; Nakatani, Kazuhiko

  Chemistry (Weinheim an der Bergstrasse, Germany), 2009

  
• Genotyping by allele-specific L-DNA-tagged PCR

  Hayashi, Gosuke; Hagihara, Masaki; Nakatani, Kazuhiko

  Journal of biotechnology, 01 Jun. 2008

  
• RNA aptamers that reversibly bind to photoresponsive peptide

  Hayashi, Gosuke; Hagihara, Masaki; Nakatani, Kazuhiko

  Nucleic acids symposium series (2004), 2008

  
• Photoregulation of a peptide-RNA interaction on a gold surface

  Hayashi, Gosuke; Hagihara, Masaki; Dohno, Chikara; Nakatani, Kazuhiko

  Journal of the American Chemical Society, 18 Jul. 2007

  
• Detection of L-DNA-tagged PCR products by surface plasmon resonance imaging.

  Hayashi G; Hagihara M; Kobori A; Nakatani K

  Chembiochem : a European journal of chemical biology, 22 Jan. 2007

  
• Reversible regulation of binding between a photoresponsive peptide and its RNA aptamer

  Hayashi, Gosuke; Hagihara, Masaki; Dohno, Chikara; Nakatani, Kazuhiko

  Nucleic acids symposium series (2004), 2007

  
• N,N'-Bis(3-aminopropyl)-2,7-diamino-1,8-naphthyridine stabilized a single pyrimidine bulge in duplex DNA.

  Suda H; Kobori A; Zhang J; Hayashi G; Nakatani K

  Bioorganic & medicinal chemistry, 15 Jul. 2005

  
• Small-molecule ligand induces nucleotide flipping in (CAG)n trinucleotide repeats.

  Nakatani K; Hagihara S; Goto Y; Kobori A; Hagihara M; Hayashi G; Kyo M; Nomura M; Mishima M; Kojima C

  Nature chemical biology, Jun. 2005

  Access this paper :

  
• Application of L-DNA as a molecular tag

  Hayashi, Gosuke; Hagihara, Masaki; Nakatani, Kazuhiko

  Nucleic acids symposium series (2004), 2005

  
• Solution structure of a small-molecular ligand complexed with CAG trinucleotide repeat DNA.

  Nakatani K; Hagihara S; Goto Y; Kobori A; Hagihara M; Hayashi G; Kyo M; Nomura M; Mishima M; Kojima C

  Nucleic acids symposium series (2004), 2005

  
• SPR fingerprinting of mismatched base pair.

  Kobori A; Peng T; Hayashi G; Nakatani K

  Nucleic acids symposium series (2004), 2004

  
• Detection of guanine-adenine mismatches by surface plasmon resonance sensor carrying naphthyridine-azaquinolone hybrid on the surface.

  Hagihara S; Kumasawa H; Goto Y; Hayashi G; Kobori A; Saito I; Nakatani K

  Nucleic acids research, 2004

  

Books and Other Publications

• Chemical protein synthesis via one-pot multiple peptide ligation strategies

  Hayashi G.; Nakatsu K.; Suzuki S.; Nakajima Y., Single work

  Amino Acids, Peptides and Proteins, 02 Feb. 2024

  

Awards

• 16 Feb. 2022

  有機合成化学奨励賞（有機合成化学協会）
• 05 Mar. 2025

  野口遵賞（公益財団法人野口研究所）
• 25 Oct. 2019

  日本ペプチド学会奨励賞（日本ペプチド学会）

External funds: Kakenhi

• 人工抗体蛍光プローブで拓く細胞の１分子科学

  Grant-in-Aid for Scientific Research (B)

  Basic Section 32010:Fundamental physical chemistry-related

  The University of Tokyo

  藤芳 暁

  From 01 Apr. 2026, To 31 Mar. 2029, Granted

  1分子イメージング;人工抗体;細胞;クライオ蛍光顕微鏡;クライオ電子顕微鏡

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26K01456/
• ケモテクノロジーを駆使した機動性DNAエレメントの解析と制御

  Grant-in-Aid for Transformative Research Areas (A)

  Transformative Research Areas, Section (III)

  Nagoya University

  林 剛介

  From 01 Apr. 2025, To 31 Mar. 2030, Granted

  翻訳後修飾;再構成ゲノム;タンパク質抗体;ヌクレオソーム

  https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-25H01300/
• Biodiversity driven by mobile DNA elements and hosts : host response and trans-generation

  Grant-in-Aid for Transformative Research Areas (A)

  Transformative Research Areas, Section (III)

  Chiba University;Kumamoto University

  石黒 啓一郎

  From 01 Apr. 2025, To 31 Mar. 2030, Granted

  トランスポゾン;反復配列;遺伝子;生物多様性;進化;ゲノム;生殖

  https://kaken.nii.ac.jp/grant/KAKENHI-ORGANIZER-25H01297/
• 膜脂質のユビキチン化とユビキチン様タンパク質修飾によるオルガネラ機能制御

  Grant-in-Aid for Scientific Research (B)

  Basic Section 43030:Functional biochemistry-related

  Juntendo University

  坂巻 純一

  From 01 Apr. 2024, To 31 Mar. 2027, Granted

  ユビキチン;リソソーム;オートファジー;ユビキチン様タンパク質;膜脂質

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K01971/
• タンパク質寿命の解析・制御研究をブーストする人工タンパク質創成技術

  Grant-in-Aid for Transformative Research Areas (A)

  Transformative Research Areas, Section (III)

  Nagoya University

  林 剛介

  From 01 Apr. 2024, To 31 Mar. 2026, Declined

  タンパク質化学合成;ユビキチン化タンパク質;抗体ミメティック;モノボディ;ペプチド化学;人工抗体;進化分子工学

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-24H01893/
• Development of single-molecule peptide sequencing method

  Grant-in-Aid for Scientific Research (S)

  Broad Section D

  Nagoya University

  村上 裕

  From 12 Apr. 2023, To 31 Mar. 2028, Granted

  ペプチド一分子シークエンス;人工抗体;進化分子工学;ペプチド;一分子配列解析;一分子ペプチド配列決定;1分子シークエンス;タンパク質

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25K24575/
• International collaboration for the development of new molecular-targeted antimicrobial agents

  Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

  Medium-sized Section 54:Internal medicine of the bio-information integration and related fields

  Kyoto University

  Kim Minsoo

  From 07 Oct. 2022, To 31 Mar. 2026, Granted

  細菌感染;細胞運動;抗菌剤

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KK0128/
• Ubiquitin Chemo-technology by Chemical Protein Synthesis and Molecular Evolution

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Complex systems

  Nagoya University

  林 剛介

  From 01 Apr. 2021, To 31 Mar. 2023, Project Closed

  人工抗体;モノボディ;タンパク質化学合成;ユビキチン化タンパク質;進化分子工学

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-21H00278/
• Mirror-image Artificial Antibody Working in Multimolecular Crowding Biosystems

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Science and Engineering

  Nagoya University

  林 剛介

  From 01 Apr. 2020, To 31 Mar. 2022, Project Closed

  タンパク質化学合成;in vitroセレクション;人工抗体;進化分子工学;分子進化工学

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-20H04704/
• Efficient Chemical Synthesis of Polyubiquitin and Ubiquitinated Proteins

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Complex systems

  Nagoya University

  林 剛介

  From 01 Apr. 2019, To 31 Mar. 2021, Project Closed

  人工抗体;タンパク質化学合成;ペプチド化学;進化分子工学;タンパク質標識化;ユビキチンプロテオミクス;in vitroセレクション

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-19H05287/
• Chemical synthesis of protein via simultaneous ligation of multiple peptide segments on DNA scaffold

  Grant-in-Aid for Scientific Research (C)

  Basic Section 37010:Bio-related chemistry

  Nagoya University

  Gosuke Hayashi

  From 01 Apr. 2018, To 31 Mar. 2021, Project Closed

  タンパク質化学合成;ペプチド連結反応;DNA templated chemistry

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K05313/
• Development of nucleic acid enzymes for new nucleic acid medicines based on the transition state in molecular degradation

  Grant-in-Aid for Scientific Research (B)

  The University of Tokyo

  Akimitsu Okamoto

  From 10 Jul. 2015, To 31 Mar. 2018, Project Closed

  RNA;アプタマー;核酸構造;バクテリア;核酸;蛍光;発酵;遷移状態;ヒュースゲン環化

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15KT0057/
• Development of a platform for histone-code research by setting nucleosomes with various modifications

  Grant-in-Aid for Young Scientists (B)

  The University of Tokyo

  Gosuke HAYASHI

  From 01 Apr. 2013, To 31 Mar. 2015, Project Closed

  ヒストン;エピジェネティクス;タンパク質化学合成;Native Chemical Ligation;翻訳後修飾;ペプチド合成;ペプチド連結反応

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25870186/