Basic Information

Academic Degree

• Ph.D.(University of Calgary)

Research History

• From Apr. 2013, To Mar. 2018

  Kyoto University, Center for iPS Cell Research and Application (CiRA)/ Hakubi Center for Advanced Research, Associate Professor
• From Apr. 2010, To Mar. 2013

  Kyoto University, Center for iPS Cell Research and Application (CiRA), Assistant Professor
• From Feb. 2009, To Mar. 2010

  Ontario Human iPS Cell Facility, The Hospital For Sick Children, iPS Cell Facility Manager
• From Sep. 2006, To Mar. 2010

  Mount Sinai Hospital, Samuel Lunenfeld Research Institute, Dr. Andras Nagy Lab, Postdoctoral Research Fellow
• From Jan. 2005, To Aug. 2006

  University of Calgary, Centre for Mouse Genomics, Molecular/Gene Targeting Consultant
• From Oct. 2001, To Mar. 2003

  Kyushu University, Faculty of Medicine, Dr.Teruhisa Tsuzuki Lab, Monbukagakusho Research Student

Language of Instruction

• Japanese
• English

ID,URL

• J-Global ID

  201401022033812100

Website(s) (URL(s))

• http://www.cira.kyoto-u.ac.jp/woltjen/

researchmap URL

• https://researchmap.jp/7000008096

Research

Research Interests

• 遺伝子ターゲティング
• SNP
• pluripotent stem cells
• transposons
• recombinases
• CRISPR
• iPS cells
• molecular genetics
• genome editing

Research Areas

• Life sciences, Genetics
• Life sciences, Developmental biology
• Life sciences, Cell biology
• Life sciences, Medical biochemistry
• Life sciences, Molecular biology
• Life sciences, Genomics

Papers

• Uniform transgene activation in Tet-On systems depends on sustained rtTA expression.

  Jun Otomo; Knut Woltjen; Hidetoshi Sakurai

  iScience, 20 Oct. 2023

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• DDX41の病的バリアントと体細胞変異の機能的差異

  中村 桃子; 蝶名林 和久; 丹羽 諒; Woltjen Knut; 越智 陽太郎; 南谷 泰仁; 小川 誠司; 高折 晃史; 吉田 善紀

  日本血液学会学術集会, Oct. 2023

  
• Highly efficient transgenic mouse production using piggyBac and its application to rapid phenotyping at the founder generation

  Eiichi Okamura; Seiya Mizuno; Shoma Matsumoto; Kazuya Murata; Yoko Tanimoto; Dinh Thi Huong Tra; Hayate Suzuki; Woojin Kang; Tomoka Ema; Kento Morimoto; Kanako Kato; Tomoko Matsumoto; Nanami Masuyama; Yusuke Kijima; Toshifumi Morimura; Fumihiro Sugiyama; Satoru Takahashi; Eiji Mizutani; Knut Woltjen; Nozomu Yachie; Masatsugu Ema

  10 Dec. 2023

  
• Enrichment of allelic editing outcomes by Prime Editing in iPS cells

  Ryo Niwa; Tomoko Matsumoto; Alexander Yusuke Liu; Maki Kawato; Takayuki Kondo; Kayoko Tsukita; Haruhisa Inoue; Thomas Luc Maurissen; Knut Woltjen

  19 Mar. 2024

  
• Novel Calmodulin Variant p.E46K Associated With Severe Catecholaminergic Polymorphic Ventricular Tachycardia Produces Robust Arrhythmogenicity in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

  Jingshan Gao; Takeru Makiyama; Yuta Yamamoto; Takuya Kobayashi; Hisaaki Aoki; Thomas L Maurissen; Yimin Wuriyanghai; Asami Kashiwa; Tomohiko Imamura; Takanori Aizawa; Hai Huang; Hirohiko Kohjitani; Misato Nishikawa; Kazuhisa Chonabayashi; Megumi Fukuyama; Hiromi Manabe; Kouichi Nakau; Tsutomu Wada; Koichi Kato; Futoshi Toyoda; Yoshinori Yoshida; Naomasa Makita; Knut Woltjen; Seiko Ohno; Nagomi Kurebayashi; Takashi Murayama; Takashi Sakurai; Minoru Horie; Takeshi Kimura

  Circulation. Arrhythmia and electrophysiology, Mar. 2023

  
• Hypoblast from human pluripotent stem cells regulates epiblast development.

  Takumi Okubo; Nicolas Rivron; Mio Kabata; Hideki Masaki; Keiko Kishimoto; Katsunori Semi; May Nakajima-Koyama; Haruko Kunitomi; Belinda Kaswandy; Hideyuki Sato; Hiromitsu Nakauchi; Knut Woltjen; Mitinori Saitou; Erika Sasaki; Takuya Yamamoto; Yasuhiro Takashima

  Nature, 05 Dec. 2023

  
• 運動器疾患に対するiPS細胞研究の進歩 関節軟骨欠損に対するiPS細胞由来軟骨を用いた治療について

  小林 与人; 武井 義明; 山下 晃弘; 森岡 美帆; 釜谷 崇志; 高才 東; Woltjen Knut; 松田 秀一; 妻木 範行

  日本整形外科学会雑誌, Aug. 2018

  
• Programmable mammalian translational modulators by CRISPR-associated proteins

  Shunsuke Kawasaki; Hiroki Ono; Moe Hirosawa; Takeru Kuwabara; Shunsuke Sumi; Suji Lee; Knut Woltjen; Hirohide Saito

  Nature Communications, 19 Apr. 2023

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• Optimization of the proliferation and persistency of CAR T cells derived from human induced pluripotent stem cells.

  Tatsuki Ueda; Sara Shiina; Shoichi Iriguchi; Seitaro Terakura; Yohei Kawai; Ryotaro Kabai; Satoko Sakamoto; Akira Watanabe; Kohei Ohara; Bo Wang; Huaigeng Xu; Atsutaka Minagawa; Akitsu Hotta; Knut Woltjen; Yasushi Uemura; Yuzo Kodama; Hiroshi Seno; Tetsuya Nakatsura; Koji Tamada; Shin Kaneko

  Nature biomedical engineering, Jan. 2023

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• mRNA-based generation of marmoset PGCLCs capable of differentiation into gonocyte-like cells

  Musashi Kubiura-Ichimaru; Christopher Penfold; Kazuaki Kojima; Constance Dollet; Haruka Yabukami; Katsunori Semi; Yasuhiro Takashima; Thorsten Boroviak; Hideya Kawaji; Knut Woltjen; Aki Minoda; Erika Sasaki; Toshiaki Watanabe

  20 Sep. 2022

  
• A stress-reduced passaging technique improves the viability of human pluripotent cells.

  Kazutoshi Takahashi; Chikako Okubo; Michiko Nakamura; Mio Iwasaki; Yuka Kawahara; Tsuyoshi Tabata; Yousuke Miyamoto; Knut Woltjen; Shinya Yamanaka

  Cell reports methods, 28 Feb. 2022

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• iPSC-based drug repositioning identifies Src/c-Abl as a therapeutic target for ALS motor neurons.

  Imamura K; Izumi Y; Watanabe A; Tsukita K; Woltjen K; Yamamoto T; Hotta A; Kondo T; Kitaoka S; Ohta A; Tanaka A; Watanabe D; Morita M; Kaji R; Takuma H; Tamaoka A; Kunath T; Wray S; Furuya H; Era T; Fujisawa T; Nishotoh H; Kengo H; Ichijo H; Julien J-P; Obata N; Hosokawa M; Akiyama H; Ayaki T; Ito H; Takahashi R; Yamanaka S; Inoue H

  Science Translational Medicine, May 2017, Peer-reviewed

  
• The oncogene-dependent resistance to reprogramming unveils cancer therapeutic targets

  Kenji Ito; Kohei Nagata; Sho Ohta; Yutaka Matsuda; Tomoyo Ukai; Ichiro Yasuda; Akira Ota; Ryota Kobayashi; Mio Kabata; Nao Sankoda; Tatsuya Maeda; Knut Woltjen; Liying Yang; Reo Maruyama; Ryohei Katayama; Takuya Yamamoto; Yasuhiro Yamada

  Cell Reports, Apr. 2022

  
• DMRT1-mediated reprogramming drives development of cancer resembling human germ cell tumors with features of totipotency

  Jumpei Taguchi; Hirofumi Shibata; Mio Kabata; Masaki Kato; Kei Fukuda; Akito Tanaka; Sho Ohta; Tomoyo Ukai; Kanae Mitsunaga; Yosuke Yamada; So I Nagaoka; Sho Yamazawa; Kotaro Ohnishi; Knut Woltjen; Tetsuo Ushiku; Manabu Ozawa; Mitinori Saitou; Yoichi Shinkai; Takuya Yamamoto; Yasuhiro Yamada

  Nature Communications, Dec. 2021

  
• Capturing human trophoblast development with naive pluripotent stem cells in vitro

  Shingo Io; Mio Kabata; Yoshiki Iemura; Katsunori Semi; Nobuhiro Morone; Atsutaka Minagawa; Bo Wang; Ikuhiro Okamoto; Tomonori Nakamura; Yoji Kojima; Chizuru Iwatani; Hideaki Tsuchiya; Belinda Kaswandy; Eiji Kondoh; Shin Kaneko; Knut Woltjen; Mitinori Saitou; Takuya Yamamoto; Masaki Mandai; Yasuhiro Takashima

  Cell Stem Cell, Apr. 2021

  
• Epithelial expression of Gata4 and Sox2 regulates specification of the squamous-columnar junction via MAPK/ERK signaling in mice.

  Nao Sankoda; Wataru Tanabe; Akito Tanaka; Hirofumi Shibata; Knut Woltjen; Tsutomu Chiba; Hironori Haga; Yoshiharu Sakai; Masaki Mandai; Takuya Yamamoto; Yasuhiro Yamada; Shinji Uemoto; Yoshiya Kawaguchi

  Nature communications, 25 Jan. 2021

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• Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation.

  Thomas L Maurissen; Knut Woltjen

  Nature communications, 08 Jun. 2020, Peer-reviewed

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• Recapitulating the human segmentation clock with pluripotent stem cells.

  Mitsuhiro Matsuda; Yoshihiro Yamanaka; Maya Uemura; Mitsujiro Osawa; Megumu K Saito; Ayako Nagahashi; Megumi Nishio; Long Guo; Shiro Ikegawa; Satoko Sakurai; Shunsuke Kihara; Thomas L Maurissen; Michiko Nakamura; Tomoko Matsumoto; Hiroyuki Yoshitomi; Makoto Ikeya; Noriaki Kawakami; Takuya Yamamoto; Knut Woltjen; Miki Ebisuya; Junya Toguchida; Cantas Alev

  Nature, Apr. 2020, Peer-reviewed

  
• Smarcb1 maintains the cellular identity and the chromatin landscapes of mouse embryonic stem cells.

  Sakakura M; Ohta S; Yagi M; Tanaka A; Norihide J; Woltjen K; Yamamoto T; Yamada Y

  Biochemical and biophysical research communications, Nov. 2019, Peer-reviewed

  
• Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations.

  Janin Grajcarek; Jean Monlong; Yoko Nishinaka-Arai; Michiko Nakamura; Miki Nagai; Shiori Matsuo; David Lougheed; Hidetoshi Sakurai; Megumu K Saito; Guillaume Bourque; Knut Woltjen

  Nature communications, 24 Oct. 2019, Peer-reviewed

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• Metalloprotease-Dependent Attenuation of BMP Signaling Restricts Cardiac Neural Crest Cell Fate

  Arai, Hiroyuki N; Sato, Fuminori; Yamamoto, Takuya; Woltjen, Knut; Kiyonari, Hiroshi; Yoshimoto, Yuki; Shukunami, Chisa; Akiyama, Haruhiko; Kist, Ralf; Sehara-Fujisawa, Atsuko

  CELL REPORTS, 15 Oct. 2019, Peer-reviewed

  
• Cell-type dependent enhancer binding of the EWS/ATF1 fusion gene in clear cell sarcomas.

  Shingo Komura; Kenji Ito; Sho Ohta; Tomoyo Ukai; Mio Kabata; Fumiaki Itakura; Katsunori Semi; Yutaka Matsuda; Kyoichi Hashimoto; Hirofumi Shibata; Masamitsu Sone; Norihide Jo; Kazuya Sekiguchi; Takatoshi Ohno; Haruhiko Akiyama; Katsuji Shimizu; Knut Woltjen; Manabu Ozawa; Junya Toguchida; Takuya Yamamoto; Yasuhiro Yamada

  Nature communications, 05 Sep. 2019, Peer-reviewed

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• De Novo DNA Methylation at Imprinted Loci during Reprogramming into Naive and Primed Pluripotency.

  Yagi M; Kabata M; Ukai T; Ohta S; Tanaka A; Shimada Y; Sugimoto M; Araki K; Okita K; Woltjen K; Hochedlinger K; Yamamoto T; Yamada Y

  Stem cell reports, May 2019, Peer-reviewed

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• Platforms of in vivo genome editing with inducibleCas9for advanced cancer modeling

  Norihide Jo; Yuko Sogabe; Yosuke Yamada; Tomoyo Ukai; Harunobu Kagawa; Kanae Mitsunaga; Knut Woltjen; Yasuhiro Yamada

  Cancer Science, Mar. 2019, Peer-reviewed

  
• Modeling the Human Segmentation Clock with Pluripotent Stem Cells

  Mitsuhiro Matsuda; Yoshihiro Yamanaka; Maya Uemura; Mitsujiro Osawa; Megumu K. Saito; Ayako Nagahashi; Megumi Nishio; Long Guo; Shiro Ikegawa; Satoko Sakurai; Shunsuke Kihara; Michiko Nakamura; Tomoko Matsumoto; Hiroyuki Yoshitomi; Makoto Ikeya; Takuya Yamamoto; Knut Woltjen; Miki Ebisuya; Junya Toguchida; Cantas Alev

  bioRxiv, Feb. 2019

  
• OVOL1 Influences the Determination and Expansion of iPSC Reprogramming Intermediates.

  Kagawa H; Shimamoto R; Kim SI; Oceguera-Yanez F; Yamamoto T; Schroeder T; Woltjen K

  Stem cell reports, Feb. 2019, Peer-reviewed

  
• Microhomology-assisted scarless genome editing in human iPSCs

  Shin-Il Kim; Tomoko Matsumoto; Harunobu Kagawa; Michiko Nakamura; Ryoko Hirohata; Ayano Ueno; Maki Ohishi; Tetsushi Sakuma; Tomoyoshi Soga; Takashi Yamamoto; Knut Woltjen

  Nature Communications, 01 Dec. 2018, Peer-reviewed

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• In vivo reprogramming drives Kras-induced cancer development

  Hirofumi Shibata; Shingo Komura; Yosuke Yamada; Nao Sankoda; Akito Tanaka; Tomoyo Ukai; Mio Kabata; Satoko Sakurai; Bunya Kuze; Knut Woltjen; Hironori Haga; Yatsuji Ito; Yoshiya Kawaguchi; Takuya Yamamoto; Yasuhiro Yamada

  Nature Communications, 01 Dec. 2018, Peer-reviewed

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• Editorial overview: Embroidering the canvas of life.

  Woltjen K; Bortvin A

  Current opinion in genetics & development, Oct. 2018, Peer-reviewed

  
• 正しい分化経路をたどったiPS細胞由来T細胞は固形腫瘍に対して優れた集積性および治療効果を示す

  上田 樹; 入口 翔一; 河合 洋平; 南川 淳隆; 三好 浩之; 寺倉 精太郎; 植村 靖史; 中面 哲也; 玉田 耕治; ウォルツェン・クヌート; 児玉 裕三; 妹尾 浩; 金子 新

  日本がん免疫学会総会プログラム・抄録集, Jul. 2018

  
• Generation of CAR-iPS-T cells expressing CD8 beta

  Ueda Tatsuki; Iriguchi Shoichi; Kawai Yohei; Minagawa Atsutaka; Miyoshi Hiroyuki; Terakura Seitaro; Uemura Yasushi; Woltjen Knut; Kodama Yuzo; Seno Hiroshi; Hitoshi Yasumichi; Nakatsura Tetsuya; Tamada Koji; Kaneko Shin

  CANCER RESEARCH, Jul. 2018, Peer-reviewed

  
• Beta-cell replacement strategies for diabetes

  Timothy J Kieffer; Knut Woltjen; Kenji Osafune; Daisuke Yabe; Nobuya Inagaki

  Journal of Diabetes Investigation, 01 May 2018, Peer-reviewed

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• Srf destabilizes cellular identity by suppressing cell-type-specific gene expression programs.

  Takashi Ikeda; Takafusa Hikichi; Hisashi Miura; Hirofumi Shibata; Kanae Mitsunaga; Yosuke Yamada; Knut Woltjen; Kei Miyamoto; Ichiro Hiratani; Yasuhiro Yamada; Akitsu Hotta; Takuya Yamamoto; Keisuke Okita; Shinji Masui

  Nature communications, 11 Apr. 2018, Peer-reviewed

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• iPSC-based CAR-T therapy targeting for GPC3

  Ueda Tatsuki; Irigchi Shoich; Kawai Youhei; Minagawa Atsutaka; Miyoshi Hiroyuki; Terakura Seitaro; Uemura Yasushi; Woltjen Knut; Kodama Yuzo; Tamada Koji; Nakatsura Tetsuya; Seno Hiroshi; Kaneko Shin

  CANCER SCIENCE, Jan. 2018, Peer-reviewed

  
• iPSC-Based Compound Screening and In Vitro Trials Identify a Synergistic Anti-amyloid beta Combination for Alzheimer's Disease

  Takayuki Kondo; Keiko Imamura; Misato Funayama; Kayoko Tsukita; Michiyo Miyake; Akira Ohta; Knut Woltjen; Masato Nakagawa; Takashi Asada; Tetsuaki Arai; Shinobu Kawakatsu; Yuishin Izumi; Ryuji Kaji; Nobuhisa Iwata; Haruhisa Inoue

  CELL REPORTS, Nov. 2017, Peer-reviewed

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• Evolutionarily Distinctive Transcriptional and Signaling Programs Drive Human Germ Cell Lineage Specification from Pluripotent Stem Cells.

  Yoji Kojima; Kotaro Sasaki; Shihori Yokobayashi; Yoshitake Sakai; Tomonori Nakamura; Yukihiro Yabuta; Fumio Nakaki; So Nagaoka; Knut Woltjen; Akitsu Hotta; Takuya Yamamoto; Mitinori Saitou

  Cell stem cell, 05 Oct. 2017, Peer-reviewed

  
• Cell-type-specific genome editing with a microRNA-responsive CRISPR-Cas9 switch

  Moe Hirosawa; Yoshihiko Fujita; Callum J. C. Parr; Karin Hayashi; Shunnichi Kashida; Akitsu Hotta; Knut Woltjen; Hirohide Saito

  NUCLEIC ACIDS RESEARCH, Jul. 2017, Peer-reviewed

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• Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency

  Masamitsu Sone; Nobuhiro Morone; Tomonori Nakamura; Akito Tanaka; Keisuke Okita; Knut Woltjen; Masato Nakagawa; John E. Heuser; Yasuhiro Yamada; Shinya Yamanaka; Takuya Yamamoto

  Cell Metabolism, May 2017, Peer-reviewed

  
• The Src/c-Abl pathway is a potential therapeutic target in amyotrophic lateral sclerosis

  Keiko Imamura; Yuishin Izumi; Akira Watanabe; Kayoko Tsukita; Knut Woltjen; Takuya Yamamoto; Akitsu Hotta; Takayuki Kondo; Shiho Kitaoka; Akira Ohta; Akito Tanaka; Dai Watanabe; Mitsuya Morita; Hiroshi Takuma; Akira Tamaoka; Tilo Kunath; Selina Wray; Hirokazu Furuya; Takumi Era; Kouki Makioka; Koichi Okamoto; Takao Fujisawa; Hideki Nishitoh; Kengo Homma; Hidenori Ichijo; Jean-Pierre Julien; Nanako Obata; Masato Hosokawa; Haruhiko Akiyama; Satoshi Kaneko; Takashi Ayaki; Hidefumi Ito; Ryuji Kaji; Ryosuke Takahashi; Shinya Yamanaka; Haruhisa Inoue

  SCIENCE TRANSLATIONAL MEDICINE, May 2017, Peer-reviewed

  
• Cellular context-dependent consequences of Apc mutations on gene regulation and cellular behavior

  Kyoichi Hashimoto; Yosuke Yamada; Katsunori Semi; Masaki Yagi; Akito Tanaka; Fumiaki Itakura; Hitomi Aoki; Takahiro Kunisada; Knut Woltjen; Hironori Haga; Yoshiharu Sakai; Takuya Yamamoto; Yasuhiro Yamada

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Jan. 2017, Peer-reviewed

  
• EWS-FLI1誘導性骨肉腫モデルによって骨分化障害が肉腫発生に重要な役割をもつことが明らかとなった

  河村 真吾; 蝉 克憲; 板倉 史晃; 柴田 博史; 堀田 秋津; Woltjen Knut; 山本 拓也; 大野 貴敏; 秋山 治彦; 山田 泰広

  日本整形外科学会雑誌, Aug. 2016, Peer-reviewed

  
• マウスEWS/FLI1誘導性骨肉腫モデルが明らかとした肉腫発生に関わる重要事項 EWS/FLI1とcancer genomeがもたらす細胞分化異常

  河村 真吾; 蝉 克憲; 板倉 史晃; 柴田 博史; 堀田 秋津; Woltjen Knut; 山本 拓也; 大野 貴敏; 秋山 治彦; 山田 泰広

  日本整形外科学会雑誌, Jun. 2016, Peer-reviewed

  
• Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives

  Fabian Oceguera-Yanez; Shin-Il Kim; Tomoko Matsumoto; Ghee Wan Tan; Long Xiang; Takeshi Hatani; Takayuki Kondo; Makoto Ikeya; Yoshinori Yoshida; Haruhisa Inoue; Knut Woltjen

  METHODS, May 2016, Peer-reviewed

  
• Report on the Conference on Transposition and Genome Engineering 2015 (TGE 2015): advancing cutting-edge genomics technology in the ancient city of Nara

  Knut Woltjen; Takashi Yamamoto; Chikara Kokubu; Junji Takeda

  GENES TO CELLS, May 2016, Peer-reviewed

  
• An EWS-FLI1-Induced Osteosarcoma Model Unveiled a Crucial Role of Impaired Osteogenic Differentiation on Osteosarcoma Development

  Shingo Komura; Katsunori Semi; Fumiaki Itakura; Hirofumi Shibata; Takatoshi Ohno; Akitsu Hotta; Knut Woltjen; Takuya Yamamoto; Haruhiko Akiyama; Yasuhiro Yamada

  STEM CELL REPORTS, Apr. 2016, Peer-reviewed

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• マウスEWS/FLI1誘導性骨肉腫モデルが明らかとした肉腫発生に関わる重要事項 EWS/FLI1とcancer genomeがもたらす細胞分化異常

  河村 真吾; 蝉 克憲; 板倉 史晃; 柴田 博史; 堀田 秋津; Woltjen Knut; 山本 拓也; 大野 貴敏; 秋山 治彦; 山田 泰広

  日本整形外科学会雑誌, Mar. 2016, Peer-reviewed

  
• At the conflux of human genome engineering and induced pluripotency

  Knut Woltjen; Fabian Oceguera-Yanez; Harunobu Kagawa; Shin-Il Kim

  Genome Editing, 01 Jan. 2016, Peer-reviewed

  
• The piggyBac Transposon as a Platform Technology for Somatic Cell Reprogramming Studies in Mouse.

  Woltjen K; Kim SI; Nagy A

  Methods in molecular biology (Clifton, N.J.), 2016, Peer-reviewed

  
• Directed Myogenic Differentiation of Human Induced Pluripotent Stem Cells.

  Emi Shoji; Knut Woltjen; Hidetoshi Sakurai

  Methods in molecular biology (Clifton, N.J.), 2016, Peer-reviewed, Invited

  
• 生体における未熟なリプログラミングの終結は絨毛がんを誘発する(Premature termination of reprogramming in vivo leads to choriocarcinoma development)

  田口 純平; 蝉 克憲; 山田 洋介; 大西 紘太郎; 柴田 博史; 光永 佳奈枝; Woltjen Knut; 山田 泰広

  日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, Dec. 2015, Peer-reviewed

  
• SS18-SSX, the Oncogenic Fusion Protein in Synovial Sarcoma, Is a Cellular Context-Dependent Epigenetic Modifier

  Sakura Tamaki; Makoto Fukuta; Kazuya Sekiguchi; Yonghui Jin; Sanae Nagata; Kazuo Hayakawa; Sho Hineno; Takeshi Okamoto; Makoto Watanabe; Knut Woltjen; Makoto Ikeya; Tomohisa Kato; Junya Toguchida

  PLOS ONE, Nov. 2015, Peer-reviewed

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• Reprogramming Roadblocks Are System Dependent

  Eleni Chantzoura; Stavroula Skylaki; Sergio Menendez; Shin-Il Kim; Anna Johnsson; Sten Linnarsson; Knut Woltjen; Ian Chambers; Keisuke Kaji

  STEM CELL REPORTS, Sep. 2015, Peer-reviewed

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• Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells.

  Sasaki K; Yokobayashi S; Nakamura T; Okamoto I; Yabuta Y; Kurimoto K; Ohta H; Moritoki Y; Iwatani C; Tsuchiya H; Nakamura S; Sekiguchi K; Sakuma T; Yamamoto T; Mori T; Woltjen K; Nakagawa M; Yamamoto T; Takahashi K; Yamanaka S

  Cell stem cell, Aug. 2015, Peer-reviewed

  
• Mutant IDH1 Dysregulates the Differentiation of Mesenchymal Stem Cells in Association with Gene-Specific Histone Modifications to Cartilage- and Bone-Related Genes

  Yonghui Jin; Hassan Elalaf; Makoto Watanabe; Sakura Tamaki; Sho Hineno; Kazuhito Matsunaga; Knut Woltjen; Yukiko Kobayashi; Sanae Nagata; Makoto Ikeya; Tomohisa Kato; Takeshi Okamoto; Shuichi Matsuda; Junya Toguchida

  PLOS ONE, Jul. 2015, Peer-reviewed

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• KLF4 N-Terminal Variance Modulates Induced Reprogramming to Pluripotency

  Shin-Il Kim; Fabian Oceguera-Yanez; Ryoko Hirohata; Sara Linker; Keisuke Okita; Yasuhiro Yamada; Takuya Yamamoto; Shinya Yamanaka; Knut Woltjen

  STEM CELL REPORTS, Apr. 2015, Peer-reviewed

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• Synthetic lateral inhibition governs cell-type bifurcation with robust ratios

  Mitsuhiro Matsuda; Makito Koga; Knut Woltjen; Eisuke Nishida; Miki Ebisuya

  NATURE COMMUNICATIONS, Feb. 2015, Peer-reviewed

  
• Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons.

  Kim SI; Oceguera-Yanez F; Sakurai C; Nakagawa M; Yamanaka S; Woltjen K

  Methods in Molecular Biology, 2015, Peer-reviewed, Invited

  
• Premature Termination of Reprogramming In Vivo Leads to Cancer Development through Altered Epigenetic Regulation

  Kotaro Ohnishi; Katsunori Semi; Takuya Yamamoto; Masahito Shimizu; Akito Tanaka; Kanae Mitsunaga; Keisuke Okita; Kenji Osafune; Yuko Arioka; Toshiyuki Maeda; Hidenobu Soejima; Hisataka Moriwaki; Shinya Yamanaka; Knut Woltjen; Yasuhiro Yamada

  CELL, Feb. 2014, Peer-reviewed

  
• Nuclease-mediated genome editing: At the front-line of functional genomics technology

  Tetsushi Sakuma; Knut Woltjen

  DEVELOPMENT GROWTH & DIFFERENTIATION, Jan. 2014, Peer-reviewed

  
• 1P135 Reconstitution of an intercellular symmetry breaking mechanism(09. Development & Differentiation,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

  Matsuda Mitsuhiro; Koga Makito; Woltjen Knut; Nishida Eisuke; Ebisuya Miki

  Seibutsu Butsuri, 2014

  
• Transient maternal IL-6 mediates long-lasting changes in neural stem cell pools by deregulating an endogenous self-renewal pathway.

  Gallagher D; Norman AA; Woodard CL; Yang G; Gauthier-Fisher A; Fujitani M; Vessey JP; Cancino GI; Sachewsky N; Woltjen K; Fatt MP; Morshead CM; Kaplan DR; Miller FD

  Cell Stem Cell, Nov. 2013, Peer-reviewed

  
• iPS細胞技術を用いた滑膜肉腫起源の同定への試み

  福田 誠; 早川 和男; 玉置 さくら; 佐藤 信吾; 池谷 真; 大塚 隆信; Woltjen Knut; 戸口田 淳也

  日本整形外科学会雑誌, Aug. 2013

  
• Cartilage tissue engineering identifies abnormal human induced pluripotent stem cells

  Akihiro Yamashita; Shiying Liu; Knut Woltjen; Bradley Thomas; Guoliang Meng; Akitsu Hotta; Kazutoshi Takahashi; James Ellis; Shinya Yamanaka; Derrick E. Rancourt

  SCIENTIFIC REPORTS, Jun. 2013, Peer-reviewed

  
• Efficient and Reproducible Myogenic Differentiation from Human iPS Cells: Prospects for Modeling Miyoshi Myopathy In Vitro

  Akihito Tanaka; Knut Woltjen; Katsuya Miyake; Akitsu Hotta; Makoto Ikeya; Takuya Yamamoto; Tokiko Nishino; Emi Shoji; Atsuko Sehara-Fujisawa; Yasuko Manabe; Nobuharu Fujii; Kazunori Hanaoka; Takumi Era; Satoshi Yamashita; Ken-ichi Isobe; En Kimura; Hidetoshi Sakurai

  PLOS ONE, Apr. 2013, Peer-reviewed

  Access this paper :

  
• Efficient TALEN construction and evaluation methods for human cell and animal applications

  Tetsushi Sakuma; Sayaka Hosoi; Knut Woltjen; Ken-ichi Suzuki; Keiko Kashiwagi; Housei Wada; Hiroshi Ochiai; Tatsuo Miyamoto; Narudo Kawai; Yasunori Sasakura; Shinya Matsuura; Yasushi Okada; Atsuo Kawahara; Shigeo Hayashi; Takashi Yamamoto

  GENES TO CELLS, Apr. 2013, Peer-reviewed

  
• Efficient TALEN construction and evaluation methods for human cell and animal applications

  Tetsushi Sakuma; Sayaka Hosoi; Knut Woltjen; Ken-Ichi Suzuki; Keiko Kashiwagi; Housei Wada; Hiroshi Ochiai; Tatsuo Miyamoto; Narudo Kawai; Yasunori Sasakura; Shinya Matsuura; Yasushi Okada; Atsuo Kawahara; Shigeo Hayashi; Takashi Yamamoto

  Genes to Cells, Apr. 2013

  
• Identification of target genes of synovial sarcoma-associated fusion oncoprotein using human pluripotent stem cells

  Kazuo Hayakawa; Makoto Ikeya; Makoto Fukuta; Knut Woltjen; Sakura Tamaki; Naoko Takahara; Tomohisa Kato; Shingo Sato; Takanobu Otsuka; Junya Toguchida

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Mar. 2013, Peer-reviewed

  
• Highly efficient site-specific transgenesis in cancer cell lines

  Iacovos P. Michael; Claudio Monetti; Anthony C. Chiu; Puzheng Zhang; Takeshi Baba; Koichiro Nishino; Siamak Agha-Mohammadi; Knut Woltjen; Hoon-Ki Sung; Andras Nagy

  MOLECULAR CANCER, Dec. 2012, Peer-reviewed

  
• Erratum to: Induced Pluripotent Stem Cell Lines Derived from Equine Fibroblasts (Stem Cell Rev and Rep, 10.1007/s12015-011-9239-5)

  Kristina Nagy; Hoon-Ki Sung; Puzheng Zhang; Simon Laflamme; Patrick Vincent; Siamak Agha-Mohammadi; Knut Woltjen; Claudio Monetti; Iacovos Prodromos Michael; Lawrence Charles Smith; Andras Nagy

  Stem Cell Reviews and Reports, Jun. 2012, Peer-reviewed

  
• An Alternative Splicing Switch Regulates Embryonic Stem Cell Pluripotency and Reprogramming

  Mathieu Gabut; Payman Samavarchi-Tehrani; Xinchen Wang; Valentina Slobodeniuc; Dave O'Hanlon; Hoon-Ki Sung; Manuel Alvarez; Shaheynoor Talukder; Qun Pan; Esteban O. Mazzoni; Stephane Nedelec; Hynek Wichterle; Knut Woltjen; Timothy R. Hughes; Peter W. Zandstra; Andras Nagy; Jeffrey L. Wrana; Benjamin J. Blencowe

  CELL, Sep. 2011, Peer-reviewed

  
• Induced Pluripotent Stem Cell Lines Derived from Equine Fibroblasts

  Kristina Nagy; Hoon-Ki Sung; Puzheng Zhang; Simon Laflamme; Patrick Vincent; Siamak Agha-Mohammadi; Knut Woltjen; Claudio Monetti; Iacovos Prodromos Michael; Lawrence Charles Smith; Andras Nagy

  STEM CELL REVIEWS AND REPORTS, Sep. 2011, Peer-reviewed

  
• PhiC31 integrase facilitates genetic approaches combining multiple recombinases

  Claudio Monetti; Koichiro Nishino; Steffen Biechele; Puzheng Zhang; Takeshi Baba; Knut Woltjen; Andras Nagy

  METHODS, Apr. 2011, Peer-reviewed

  
• Transposon-based production of genetically unmodified induced pluripotent stem cells from human embryonic fibroblasts

  Woltjen K; Hamalainen R; Kibschull M; Mileikovsky M; Nagy A

  Methods in Molecular Biology 767 (87):103, 2011, Peer-reviewed

  
• Transgene-free production of pluripotent stem cells using piggyBac transposons

  Knut Woltjen; Riikka Hämäläinen; Mark Kibschull; Maria Mileikovsky; Andras Nagy

  Methods in Molecular Biology, 2011, Peer-reviewed

  
• Functional Genomics Reveals a BMP-Driven Mesenchymal-to-Epithelial Transition in the Initiation of Somatic Cell Reprogramming

  Payman Samavarchi-Tehrani; Azadeh Golipour; Laurent David; Hoon-Ki Sung; Tobias A. Beyer; Alessandro Datti; Knut Woltjen; Andras Nagy; Jeffrey L. Wrana

  CELL STEM CELL, Jul. 2010, Peer-reviewed

  
• Obtaining Pluripotency Biomarker Signatures

  Kathryn Sciabica; Knut Woltjen; Akitsu Hotta

  GENETIC ENGINEERING & BIOTECHNOLOGY NEWS, Feb. 2010

  
• Inhibition of Tgf-beta Signaling Improves Mouse Fibroblast Reprogramming

  Knut Woltjen; William L. Stanford

  CELL STEM CELL, Nov. 2009, Peer-reviewed

  
• New strategies to generate induced pluripotent stem cells

  James O'Malley; Knut Woltjen; Keisuke Kaji

  CURRENT OPINION IN BIOTECHNOLOGY, Oct. 2009, Peer-reviewed

  
• Virus-free induction of pluripotency and subsequent excision of reprogramming factors

  Keisuke Kaji; Katherine Norrby; Agnieszka Paca; Maria Mileikovsky; Paria Mohseni; Knut Woltjen

  NATURE, Apr. 2009, Peer-reviewed

  
• piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells

  Knut Woltjen; Iacovos P. Michael; Paria Mohseni; Ridham Desai; Maria Mileikovsky; Riikka Haemaelaeinen; Rebecca Cowling; Wei Wang; Pentao Liu; Marina Gertsenstein; Keisuke Kaji; Hoon-Ki Sung; Andras Nagy

  NATURE, Apr. 2009, Peer-reviewed

  
• Orpheus recombination: A comprehensive bacteriophage system for murine targeting vector construction by transplacement

  Knut Woltjen; Kenichi Ito; Teruhisa Tsuzuki; Derrick E. Rancourt

  Methods in Molecular Biology, 2008, Peer-reviewed

  
• Deep screening of recombination proficient bacteriophage libraries

  B Thomas; K Woltjen; DE Rancourt

  BIOTECHNIQUES, Jan. 2003, Peer-reviewed

  
• Bacteriophage gene targeting vectors generated by transplacement

  C Aoyama; K Woltjen; FC Mansergh; K Ishidate; DE Rancourt

  BIOTECHNIQUES, Oct. 2002, Peer-reviewed

  
• Transplacement mutagenesis. A recombination-based in situ mutagenesis protocol.

  K. Woltjen; M.W. Unger; D.E. Rancourt

  Methods in molecular biology (Clifton, N.J.), 2002, Peer-reviewed, Invited

  
• Retro-recombination screening of a mouse embryonic stem cell genomic library.

  K. Woltjen; G. Bain; D.E. Rancourt

  Nucleic acids research, 2000, Peer-reviewed

  

Misc.

• EWS/ATF1融合遺伝子は細胞種特異的なエンハンサーに結合して明細胞肉腫を発生させる

  河村真吾; 伊藤謙治; 大野貴敏; 清水克時; WOLTJEN Knut; 小沢学; 戸口田淳也; 山本拓也; 秋山治彦; 山田泰広

  日本整形外科学会雑誌, 2022

  
• EWS/ATF1融合遺伝子は細胞種特異的なエンハンサーに結合して明細胞肉腫を発生させる

  河村真吾; 伊藤謙治; 大野貴敏; 清水克時; WOLTJEN Knut; 小沢学; 戸口田淳也; 山本拓也; 秋山治彦; 山田泰広

  日本整形外科学会雑誌, 2020

  
• 腱および腱周囲結合組織を標識する遺伝子改変マウスの樹立

  河村真吾; 平川明弘; 蝉克憲; WOLTJEN Knut; 山田泰広; 秋山治彦

  日本整形外科学会雑誌, 2016

  
• 正しい分化経路をたどったiPS細胞由来T細胞は固形腫瘍に対して優れた集積性および治療効果を示す

  上田 樹; 入口 翔一; 河合 洋平; 南川 淳隆; 三好 浩之; 寺倉 精太郎; 植村 靖史; 中面 哲也; 玉田 耕治; ウォルツェン・クヌート; 児玉 裕三; 妹尾 浩; 金子 新

  日本がん免疫学会総会プログラム・抄録集, Jul. 2018

  
• GPC3を標的としたiPS細胞由来CAR-T療法

  上田 樹; 入口 翔一; 河合 洋平; 南川 淳隆; 三好 浩之; 寺倉 精太郎; 植村 靖史; ウォルツェン・クヌート; 児玉 裕三; 玉田 耕治; 中面 哲也; 妹尾 浩; 金子 新

  日本癌学会総会記事, Sep. 2017

  
• 変異型IDH1は遺伝子特異的なヒストン修飾を介して、間葉系幹細胞から軟骨及び骨への分化を脱制御する

  金 永輝; Elalaf Hassan; 渡辺 真; 玉置 さくら; 日根野 翔; 松永 一仁; Woltjen Knut; 岡本 健; 松田 秀一; 戸口田 淳也

  日本癌学会総会記事, Oct. 2016

  
• 変異型IDH1は遺伝子特異的なヒストン修飾を介して、間葉系幹細胞から軟骨及び骨への分化を脱制御する

  金 永輝; Elalaf Hassan; 渡辺 真; 玉置 さくら; 日根野 翔; 松永 一仁; Woltjen Knut; 岡本 健; 松田 秀一; 戸口田 淳也

  日本癌学会総会記事, Oct. 2016

  
• 変異型IDH1は骨・軟骨関連遺伝子のヒストンを遺伝子特異的に修飾し、間葉系幹細胞の分化能を脱制御する

  金 永輝; Elalaf Hassan; 渡辺 真; 玉置 さくら; 日根野 翔; 松永 一仁; Woltjen Knut; 岡本 健; 松田 秀一; 戸口田 淳也

  日本癌学会総会記事, Oct. 2015

  
• Engineered Nuclease Mediated Genetic Correction in iPSCs Derived From Duchenne Muscular Dystrophy Patient

  Hongmei Lisa Li; Naoko Fujimoto; Noriko Sasakawa; Saya Shirai; Takashi Yamamoto; Knut Woltjen; Akira Watanabe; Hidetoshi Sakurai; Shinya Yamanaka; Akitsu Hotta

  MOLECULAR THERAPY, May 2014

  
• 【ゲノム編集革命:遺伝子改変はZFN・TALEN・CRISPR/Cas三強時代へ】 TALENを用いたヒトiPS細胞におけるゲノム編集

  李 紅梅; Woltjen Knut; 高橋 和利; 山中 伸弥; 堀田 秋津

  細胞工学, Apr. 2013, Invited

  
• Application of iPS cell technology \nfor the analysis of origin-unknown sarcoma characterized by a specific fusion gene．

  Hayakawa K; Fukuta M; TamaiS; Kato T; Ikeya M; Otsuka T; Woltjen K; Toguchida J

  第17 回CTOS （2012.11.16．Prague）, 16 Nov. 2012, Peer-reviewed

  
• Induced Pluripotent Stem Cell Lines Derived from Equine Fibroblasts (vol 7, pg 693, 2011)

  Kristina Nagy; Hoon-Ki Sung; Puzheng Zhang; Simon Laflamme; Patrick Vincent; Siamak Agha-Mohammadi; Knut Woltjen; Claudio Monetti; Iacovos Prodromos Michael; Lawrence Charles Smith; Andras Nagy

  STEM CELL REVIEWS AND REPORTS, Jun. 2012

  
• デュシェンヌ型筋ジストロフィー患者由来iPS 細胞を用いての病態再現

  庄子栄美; 櫻井英俊; 中畑龍俊; 田中章仁; ウォルツェンクヌート; 藤井宣晴; 平家敏男; 真鍋康子; 瀬原-藤沢淳子

  第35 回日本分子生物学会年会（2012.12.12．福岡）, 2012, Peer-reviewed

  
• MYF5遺伝子発現制御によるヒトiPS細胞の骨格筋分化誘導の検討

  近藤潤一; 田中章仁; WOLTJEN Knut; 沖田圭介; 櫻井英俊

  日本分子生物学会年会プログラム・要旨集(Web), 2012

  
• Establishment and functional \nanalysis of iPS cells with drug-inducible SYT-SSX fusion gene．

  Fukuta M; Hayakawa K; Tamaki S; Sato S; Ikeya M; Otsuka Y; Woltjen K; Toguchida J

  第10 回ISSCR（2012.6.14．横浜）, 2012, Peer-reviewed

  
• PS 細胞を用いた肉腫研究：滑膜肉腫の起源細胞の解明をめざして

  福田誠; 早川和男; 玉置さくら; 沖田圭介; 池谷真; Knut Woltjen; 大塚隆信; 戸口田淳也

  第27 回日本整形外科学会基礎学術集会（2012.10.26． 名古屋）, 2012, Peer-reviewed

  
• 薬剤誘導型発現ベクターを用いたSYT-SSX 融合遺伝子導入iPS 細胞の樹立

  早川和男; 福田誠; 玉置さくら; 佐藤信吾; 池谷真; 大塚隆信; Knut Woltjen; 戸口田淳也

  第45 回日本整形外科学会骨軟部腫瘍学術 集会（2012.7.15．東京）, 2012, Peer-reviewed

  

Presentations

• Precise Human Disease Allele Creation and Correction through Microhomology-Mediated End Joining

  Knut Woltjen

  2019 Keystone Symposia 『Genome Engineering: From Mechanisms to Therapies (B4)』, 21 Feb. 2019, Invited

  
• The history and potential of iPSC research

  Knut Woltjen

  AAAS 2019 Annual Meeting, 15 Feb. 2019, Invited

  
• 医療応用に向けたヒトiPS細胞でのゲノム編集

  ウォルツェン クヌート

  OPERA「ゲノム編集」産学共創コンソーシアム・公開シンポジウム、卓越大学院キックオフシンポジウム, 10 Dec. 2018, Invited

  
• Japan’s experience in regenerative medicine and superdonor cells

  Knut Woltjen

  NRC’s HEALTH CHALLENGE PROGRAM WORKSHOP : ‘Transforming Health Outcomes through Engineered Cell and Gene Therapies’, 07 Dec. 2018, Invited

  
• Precise Human Disease Allele Creation and Correction through Microhomology-Mediated End Joining

  Knut Woltjen

  Concordia University Seminar, 06 Dec. 2018, Invited

  
• Precise human disease allele creation and correction through microhomology-mediated end joining

  Knut Woltjen

  The 41st Annual Meeting of the Molecular Biology Society of Japan, 28 Nov. 2018, Invited

  
• Genome editing in stem cells to study the mechanisms of reprogramming and human disease

  Knut Woltjen

  Basel Stem Cell Network (BSCN) International Lecture Series（＠ETH Zürich, D-BSSE）, 14 Sep. 2018, Invited

  
• Creation and correction of human disease alleles in iPSCs through microhomology-mediated end joining

  Knut Woltjen

  EMBO at BASEL LIFE 2018, 13 Sep. 2018

  
• Precise human disease allele creation and correction through microhomology-mediated end joining

  Knut Woltjen

  IREM seminar（ University of Zurich）, 12 Sep. 2018, Invited

  
• Precise human disease allele creation and correction through microhomology-mediated end joining

  Knut Woltjen

  Genome Engineering: The CRISPR-Cas Revolution, 25 Aug. 2018, Invited

  
• Employing microhomology-mediated end joining for the creation and correction of human disease alleles

  Knut Woltjen

  Developmental Biology Guest Research Seminar(Memorial Sloan Kettering Cancer Center), 22 Aug. 2018, Invited

  
• Scarless editing of human disease alleles through microhomology-mediated end joining (MMEJ)

  Knut Woltjen

  3rd Annual Meeting of The Japanese Society for Genome Editing, 19 Jun. 2018, Invited

  
• GENES AND CELLS: THE LIVING DRUGS OF A NEW MEDICAL ERA

  Knut Woltjen

  "Stem Cells and Regenerative Medicine: What does the Future Hold?" （The University of British Columbia ), 29 Mar. 2018, Invited

  
• The development of induced pluripotent stem cells and emerging tools for genome editing

  Knut Woltjen

  "Stem Cells and Regenerative Medicine: What does the Future Hold?" （The University of British Columbia ), 28 Mar. 2018, Invited

  
• Creation and correction of human disease alleles through microhomology-mediated end joining (MMEJ)

  Knut Woltjen

  The 9th Takeda Science Foundation Symposium on PharmaSciences, 07 Feb. 2018, Invited

  
• Endogenous DNA repair pathways for gene editing in human induced pluripotent stem cells

  Knut Woltjen

  Celllular & Physiological Sciences Departmental Seminar（The University of British Columbia ), 14 Sep. 2017, Invited

  
• Genetic engineering of iPS cells for modelling genodermatoses

  Knut Woltjen

  The 38th Annual Meeting of the Japanese Society of Inflammation and Regeneration, 19 Jul. 2017, Invited

  
• Simultaneous derivation of point-mutants and concordant isogenic clones from human iPSCs

  Knut Woltjen

  2nd Annual Meeting of The Japanese Society for Genome Editing, 29 Jun. 2017, Invited

  
• Genetic engineering of pluripotent stem cells by CRISPR/Cas

  Knut Woltjen

  糖尿病ミニシンポジウム「Kyoto Diabetes Mini-Symposium – Beta-Cell Replacement Strategies」, 05 Jun. 2017, Invited

  
• Engineering iPS cell models of human disease

  Knut Woltjen

  The 75th Annual Meeting of the Japanese Cancer Association, 07 Oct. 2016, Invited

  
• An epithelial program driven by KLF4 stoichiometry determines hallmarks of somatic cell reprogramming

  Knut Woltjen

  Liaison Laboratory Regular Seminar/HIGO Program Cutting-edge Seminar, 17 Feb. 2016, Invited

  
• An epithelial program driven by KLF4 stoichiometry determines hallmarks of somatic cell reprogramming

  Knut Woltjen

  九州大学大学院医学研究院 第３５回生命医科学セミナー, 16 Feb. 2016, Invited

  
• At the Conflux of Genome Engineering and Pluripotent Stem Cell Biology

  Knut Woltjen

  Conference on Transposition and Genome Engineering 2015, 20 Nov. 2015, Invited

  
• Engineering the Human Genome: Strategies for iPS Cell Derivation and Disease Modeling

  Knut Woltjen

  Wakate International Symposium “Discovery of Medical Sciences,” (WISDoMS), 06 Nov. 2014, Invited

  
• From Transposons to Nucleases: Genome Engineering Strategies for iPS Cell Derivation and Disease Modeling

  Knut Woltjen

  The 71st of Stem Cell Biology and Regenerative Medicine Forum, 30 Jun. 2014, Invited

  
• Recombinant DNA and Cell Technologies Enabling Human Functional Genomics

  Knut Woltjen

  Tokyo Tech Bio 2013, 29 Jan. 2014, Invited

  
• Recombinant DNA and Cell Technologies Enabling Human Functional Genomics

  Knut Woltjen

  Department of Biological Sciences Molecules, Cells, and Systems Seminar Series (University of Alberta), 04 Nov. 2013, Invited

  
• Transposed and targeted expression systems for guiding somatic cell reprogramming and differentiation

  Knut Woltjen

  Transposition and Genome Engineering 2013, Sep. 2013, Invited

  
• Transposed and Targeted Gene Expression Systems for Guiding Somatic Cell Reprogramming and Differentiation

  Knut Woltjen

  46th Annual Meeting of the Japanese Society of Development Biologists, 31 May 2013, Invited

  
• Cell Identity Transitions During Induced Reprogramming to Pluripotency

  Knut Woltjen

  The 7th Annual Meeting of the Japanese Society for Epigenetics, May 2013, Invited

  
• Cell Identity Transitions During Induced Reprogramming to Pluripotency

  Knut Woltjen

  CDB Seminar, 29 Mar. 2013, Invited

  
• Transposon Systems to Guiding Somatic Cell Reprogramming and Differentiation

  Knut Woltjen

  iCeMS-Stem Cells Australia Joint Symposium, 12 Feb. 2013, Invited

  

Books and Other Publications

• iPS細胞を用いた正確な ゲノム編集法(MhAX法)

  Harunobu Kagawa; Tomoko Matsumoto; Shin-Il Kim; Knut Woltjen, Contributor, pp. 1-8

  実験医学, Oct. 2018, Not refereed

  

Industrial Property Rights

• 特願PCT/IB2017/054736, Method for genome editing

  Knut Woltjen; Shin-Il Kim; Tomoko Matsumoto

  

External funds: Kakenhi

• 肺オルガノイドを用いた呼吸器病原体の革新的研究基盤の構築と病原性発現機序の解明

  Grant-in-Aid for Scientific Research (B)

  Basic Section 54030:Infectious disease medicine-related

  National Institute of Infectious Diseases

  酒井 宏治

  From 01 Apr. 2021, To 31 Mar. 2024, Granted

  SARS-CoV-2;インフルエンザウイルス;iPS細胞;ACE2;TMPRSS2;肺オルガノイド;ゲノム編集

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H02973/
• Evaluating the role of cis-regulatory tandem DNA repeats in human disease and evolution

  Grant-in-Aid for Scientific Research (B)

  Basic Section 43050:Genome biology-related

  Kyoto University

  ウォルツェン クヌート

  From 01 Apr. 2021, To 31 Mar. 2024, Granted

  DNAリピート;ゲノム編集;CRISPR-Cas9;iPS細胞;レポーターアッセイ;ゲノム解析;ヒトiPS細胞

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H02460/
• Developing a versatile technique to create transgenic rodents and medium sized animals

  Grant-in-Aid for Scientific Research (B)

  Basic Section 42040:Laboratory animal science-related

  Shiga University of Medical Science

  依馬 正次

  From 01 Apr. 2021, To 31 Mar. 2024, Granted

  トランスポゾン;トランスジェニック;トランスポザーゼ

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H02388/
• Regulation of gene expression revealed by genome editing of repetitive and polymorphic cis-elements

  Grant-in-Aid for JSPS Fellows

  Basic Section 43050:Genome biology-related

  Kyoto University

  ウォルツェン クヌート

  From 18 Nov. 2021, To 31 Mar. 2023, Project Closed

  ゲノム編集;ゲノム解析;DNAリピート;ヒトiPS細胞;genome editing;genome analysis;DNA repeat;human iPS cells

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21F21382/
• Establishment of universal iPS cells for regenerative medicine applications through regulated HLA expression

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Knut Woltjen

  From 01 Apr. 2017, To 31 Mar. 2020, Project Closed

  Human leukocyte antigen;HLA;ゲノム編集;iPS細胞;CRISPR Cas9;KRAB;B2M;allograft;alloresponse;再生医療;ゲノム生物工学;個別化医療

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K07256/
• CRISPR/Cas9 mediated identification of novel gene targets for XPA-associated skin cancer using in vitro reconstituted skin from iPS cells

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Fabian Oceguera-Yanez

  From 01 Apr. 2017, To 31 Mar. 2020, Project Closed

  Human iPSCs;CRISPR/Cas9;Squamous Cell Carcinoma;Skin differentiation;Xeroderma pigmentosum

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K07165/
• Induction of tendon/ligament progenitor cells from human iPSCs

  Grant-in-Aid for Challenging Exploratory Research

  Kyoto University

  CANTAS ALEV

  From 01 Apr. 2016, To 31 Mar. 2018, Project Closed

  Tendon Progenitor Cell;iPSCs;Mesoderm Differentiation;tendon progenitor cells;mesoderm differentiation;syndetome;Tendon Progenitor Cells;iPS細胞;腱誘導;中胚葉誘導;スクレラキシス;リポーター細胞

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K15664/
• Single nucleotide-level genome engineering using transposon cassettes

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  Knut WOLTJEN

  From 01 Apr. 2011, To 31 Mar. 2013, Project Closed

  遺伝子ターゲティング;ヌクレアーゼ;iPS細胞;TALEN nuclease;transposon;cardiomyocyte;SNP;ion channel;Gene targeting;CRISPR/Cas9 nuclease;iPS Cell;long QT-syndrome;TALEヌクレアーゼ;トランスポゾン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23710240/
• Molecular mechanisms underlying nuclear reprogramming of somatic cells

  Grant-in-Aid for Specially Promoted Research

  Biological Sciences

  Kyoto University

  Shinya YAMANAKA

  From 01 Apr. 2007, To 31 Mar. 2012, Project Closed

  再生医学;幹細胞;分化多能性;核初期化;転写因子

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19002014/
• ゲノム編集を駆使したシスエレメントの反復配列多型による遺伝子発現制御の理解

  Grant-in-Aid for JSPS Fellows

  Basic Section 43050:Genome biology-related

  Kyoto University

  ウォルツェン クヌート

  From 25 Apr. 2023, To 31 Mar. 2024, Granted

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23KF0041/
• ゲノム編集を駆使したシスエレメントの反復配列多型による遺伝子発現制御の理解

  Grant-in-Aid for JSPS Fellows

  Basic Section 43050:Genome biology-related

  Kyoto University

  ウォルツェン クヌート

  From 25 Apr. 2023, To 31 Mar. 2024, Project Closed

  DNAリピート;ゲノム編集;CRISPR-Cas9;iPS細胞;レポーターアッセイ

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23KF0041/
• Evaluating the role of cis-regulatory tandem DNA repeats in human disease and evolution

  Grant-in-Aid for Scientific Research (B)

  Basic Section 43050:Genome biology-related

  Kyoto University

  Knut Woltjen

  From 01 Apr. 2021, To 31 Mar. 2024, Project Closed

  ゲノム編集;ゲノム解析;DNAリピート;ヒトiPS細胞;CRISPR-Cas9;iPS細胞;レポーターアッセイ

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H02460/
• Developing a versatile technique to create transgenic rodents and medium sized animals

  Grant-in-Aid for Scientific Research (B)

  Basic Section 42040:Laboratory animal science-related

  Shiga University of Medical Science

  Masatsugu Ema

  From 01 Apr. 2021, To 31 Mar. 2024, Project Closed

  トランスポゾンベクター;トランスジェニック;トランスポザーゼ;トランスポゾン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H02388/

External funds: others

• Immediate Early Events in Reprogramming Leading to Cellular Plasticity

  From 2010, To 2012

Education

Teaching subject(s)

• From 01 Apr. 2024, To 31 Mar. 2025

  Global Frontier in Life Science A

  1018, Spring, Graduate School of Biostudies, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Global Frontier in Life Science A

  1018, Spring, Graduate School of Biostudies, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Global Frontier in Life Science A

  1018, Spring, Graduate School of Biostudies, 2
• From Apr. 2013, To Mar. 2014

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2014, To Mar. 2015

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2015, To Mar. 2016

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2016, To Mar. 2017

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2017, To Mar. 2018

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2018, To Mar. 2019

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2019, To Mar. 2020

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2020, To Mar. 2021

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Global Frontier in Life Science A

  Spring, 生命科学研究科

Administration

Faculty management (title, position)

• From 01 Apr. 2022, To 01 Apr. 2025

  白眉センター専門委員会
• From 26 May 2022, To 31 Mar. 2026

  創薬技術開発室運営委員会
• From 01 Apr. 2018, To 31 Mar. 2020

  組換えDNA実験安全委員会4号委員

Academic, Social Contribution

Committee Memberships

• From Jul. 2018, To Present

  International Committee, Japanese Society for Genome Editing
• From Jul. 2018, To Present

  国際委員会委員, 日本ゲノム編集学会
• From Jul. 2016, To Present

  Public Relations Committee, Japanese Society for Genome Editing
• From Jul. 2016, To Present

  広報委員会委員, 日本ゲノム編集学会