Basic Information

Academic Degree

• 23 Mar. 2009

  京都大学修士（医科学）
• 24 Mar. 2014

  京都大学博士（医科学）

Academic Resume (Graduate Schools)

• 京都大学, 大学院医学研究科博士後期課程医科学専攻, 修了
• 京都大学, 大学院医学研究科博士後期課程医科学専攻, 研究指導認定退学
• 京都大学, 大学院医学研究科修士課程医科学専攻, 修了

Academic Resume (Undergraduate School/Majors)

• 京都大学, 農学部資源生物科学科, 卒業

Research History

• From Apr. 2023, To Present

  Kyoto University, Hakubi Center, Assistant professor, Hakubi Researcher
• From Dec. 2022, To Mar. 2023

  Kyoto University, Institute for the Advanced Study of Human Biology (ASHBi), Program-specific Researcher
• From Jun. 2014, To Nov. 2022

  Ludwig Maximilian University of Munich, Department Biology, Anthropology and Human Genomics, Post-Doc Researcher
• From Apr. 2015, To Mar. 2017

  Japan Society for the Promotion of Science, Overseas Fellow
• From Apr. 2012, To May 2014

  Kyoto University, Center for iPS Cell Research and Application (CiRA), Program-specific Researcher
• From Apr. 2009, To Mar. 2012

  Japan Society for the Promotion of Science, Research Fellow DC1

ID,URL

• ORCID ID

  0000-0001-5478-6297

• J-Global ID

  202301017709930050

• Researcher number

  40983606

researchmap URL

• https://researchmap.jp/ohnukimari

Research

Research Interests

• Regulatory element
• Evolution
• Primate
• Transposable element
• iPS cell
• Reprogramming

Papers

• Evidence for compensatory evolution within pleiotropic regulatory elements

  Zane Kliesmete; Peter Orchard; Victor Yan Kin Lee; Johanna Geuder; Simon M. Krauß; Mari Ohnuki; Jessica Jocher; Beate Vieth; Wolfgang Enard; Ines Hellmann

  Genome Research, Oct. 2024

  
• Evidence for compensatory evolution within pleiotropic regulatory elements

  Zane Kliesmete; Peter Orchard; Victor Yan Kin Lee; Johanna Geuder; Simon M. Krauß; Mari Ohnuki; Jessica Jocher; Beate Vieth; Wolfgang Enard; Ines Hellmann

  11 Jan. 2024

  
• Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi.

  Fiona C Edenhofer; Anita Térmeg; Mari Ohnuki; Jessica Jocher; Zane Kliesmete; Eva Briem; Ines Hellmann; Wolfgang Enard

  iScience, 21 Jun. 2024, Peer-reviewed

  
• Generation of chimpanzee induced pluripotent stem cell lines for cross-species comparisons.

  Masanori Imamura; Risako Nakai; Mari Ohnuki; Yusuke Hamazaki; Hideyuki Tanabe; Momoka Sato; Yu Harishima; Musashi Horikawa; Mao Watanabe; Hiroki Oota; Masato Nakagawa; Shunsuke Suzuki; Wolfgang Enard

  In vitro cellular & developmental biology. Animal, 22 Feb. 2024, Peer-reviewed

  
• Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine.

  Jessica Radmer; Johanna Geuder; Fiona C Edenhofer; Wolfgang Enard; Mari Ohnuki

  Journal of visualized experiments : JoVE, 28 Jul. 2023, Peer-reviewed

  
• Regulatory and coding sequences of TRNP1 co-evolve with brain size and cortical folding in mammals.

  Zane Kliesmete; Lucas Esteban Wange; Beate Vieth; Miriam Esgleas; Jessica Radmer; Matthias Hülsmann; Johanna Geuder; Daniel Richter; Mari Ohnuki; Magdelena Götz; Ines Hellmann; Wolfgang Enard

  eLife, 22 Mar. 2023, Peer-reviewed

  
• The pluripotent stem cell-specific transcript ESRG is dispensable for human pluripotency.

  Kazutoshi Takahashi; Michiko Nakamura; Chikako Okubo; Zane Kliesmete; Mari Ohnuki; Megumi Narita; Akira Watanabe; Mai Ueda; Yasuhiro Takashima; Ines Hellmann; Shinya Yamanaka

  PLoS genetics, May 2021, Peer-reviewed

  Access this paper :

  
• A non-invasive method to generate induced pluripotent stem cells from primate urine.

  Johanna Geuder; Lucas E Wange; Aleksandar Janjic; Jessica Radmer; Philipp Janssen; Johannes W Bagnoli; Stefan Müller; Artur Kaul; Mari Ohnuki; Wolfgang Enard

  Scientific reports, 10 Feb. 2021, Peer-reviewed

  
• Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata).

  Risako Nakai; Mari Ohnuki; Kota Kuroki; Haruka Ito; Hirohisa Hirai; Ryunosuke Kitajima; Toko Fujimoto; Masato Nakagawa; Wolfgang Enard; Masanori Imamura

  Scientific reports, 15 Aug. 2018, Peer-reviewed

  Access this paper :

  
• Epigenetic Variation between Human Induced Pluripotent Stem Cell Lines Is an Indicator of Differentiation Capacity.

  Masatoshi Nishizawa; Kazuhisa Chonabayashi; Masaki Nomura; Azusa Tanaka; Masahiro Nakamura; Azusa Inagaki; Misato Nishikawa; Ikue Takei; Akiko Oishi; Koji Tanabe; Mari Ohnuki; Hidaka Yokota; Michiyo Koyanagi-Aoi; Keisuke Okita; Akira Watanabe; Akifumi Takaori-Kondo; Shinya Yamanaka; Yoshinori Yoshida

  Cell stem cell, 01 Sep. 2016

  
• Present and future challenges of induced pluripotent stem cells.

  Mari Ohnuki; Kazutoshi Takahashi

  Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 19 Oct. 2015

  
• Dynamic regulation of human endogenous retroviruses mediates factor-induced reprogramming and differentiation potential.

  Mari Ohnuki; Koji Tanabe; Kenta Sutou; Ito Teramoto; Yuka Sawamura; Megumi Narita; Michiko Nakamura; Yumie Tokunaga; Masahiro Nakamura; Akira Watanabe; Shinya Yamanaka; Kazutoshi Takahashi

  Proceedings of the National Academy of Sciences of the United States of America, 26 Aug. 2014

  
• Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm.

  Kazutoshi Takahashi; Koji Tanabe; Mari Ohnuki; Megumi Narita; Aki Sasaki; Masamichi Yamamoto; Michiko Nakamura; Kenta Sutou; Kenji Osafune; Shinya Yamanaka

  Nature communications, 24 Apr. 2014

  
• Differentiation-defective phenotypes revealed by large-scale analyses of human pluripotent stem cells.

  Michiyo Koyanagi-Aoi; Mari Ohnuki; Kazutoshi Takahashi; Keisuke Okita; Hisashi Noma; Yuka Sawamura; Ito Teramoto; Megumi Narita; Yoshiko Sato; Tomoko Ichisaka; Naoki Amano; Akira Watanabe; Asuka Morizane; Yasuhiro Yamada; Tosiya Sato; Jun Takahashi; Shinya Yamanaka

  Proceedings of the National Academy of Sciences of the United States of America, 17 Dec. 2013

  
• Variation in the safety of induced pluripotent stem cell lines.

  Kyoko Miura; Yohei Okada; Takashi Aoi; Aki Okada; Kazutoshi Takahashi; Keisuke Okita; Masato Nakagawa; Michiyo Koyanagi; Koji Tanabe; Mari Ohnuki; Daisuke Ogawa; Eiji Ikeda; Hideyuki Okano; Shinya Yamanaka

  Nature biotechnology, Aug. 2009

  
• Generation and characterization of human induced pluripotent stem cells.

  Mari Ohnuki; Kazutoshi Takahashi; Shinya Yamanaka

  Current protocols in stem cell biology, Jun. 2009

  
• Induction of pluripotent stem cells from adult human fibroblasts by defined factors.

  Kazutoshi Takahashi; Koji Tanabe; Mari Ohnuki; Megumi Narita; Tomoko Ichisaka; Kiichiro Tomoda; Shinya Yamanaka

  Cell, 30 Nov. 2007

  
• Reprogramming events of mammalian somatic cells induced by Xenopus laevis egg extracts.

  Kei Miyamoto; Tadashi Furusawa; Mari Ohnuki; Sandeep Goel; Tomoyuki Tokunaga; Naojiro Minami; Masayasu Yamada; Keita Ohsumi; Hiroshi Imai

  Molecular reproduction and development, Oct. 2007

  
• Nuclear reprogramming of porcine cells and their use as donor cell for nuclear transfer after treatment in Xenopus egg extracts

  K. Miyamoto; M. Ohnuki; N. Minami; M. Yamada; H. Imai

  REPRODUCTION FERTILITY AND DEVELOPMENT, 2007, Peer-reviewed

  
• A defect in protein farnesylation suppresses a loss of Schizosaccharomyces pombe tsc2+, a homolog of the human gene predisposing to tuberous sclerosis complex.

  Yukiko Nakase; Keiko Fukuda; Yuji Chikashige; Chihiro Tsutsumi; Daisuke Morita; Shinpei Kawamoto; Mari Ohnuki; Yasushi Hiraoka; Tomohiro Matsumoto

  Genetics, Jun. 2006

  

Misc.

• A defect in protein farnesylation suppresses a loss of Schizosaccharomyces pombe tsc2(+), a homolog of the human gene predisposing to tuberous sclerosis complex (vol 173, pg 569, 2006)

  Y. Nakase; K. Fukuda; Y. Chikashige; C. Tsutsumi; D. Morita; S. Kawamoto; M. Ohnuki; Y. Hiraoka; T. Matsumoto

  GENETICS, Aug. 2006

  

Industrial Property Rights

• 特許第6143265号, 特願2014-503890, 人工多能性幹細胞の選別方法

  山中 伸弥; 高橋 和利; 小柳 三千代; 大貫 茉里

  
• 特表2016-520288, 特願2015-560441, 効率的な人工多能性幹細胞の樹立方法

  山中 伸弥; 高橋 和利; 田邊 剛士; 大貫 茉里

  
• 特許第5936134号, 特願2012-556312, ヒト人工多能性幹細胞の選択方法

  山中 伸弥; 高橋 和利; 大貫 茉里

  
• 特許第5682043号, 特願2012-512108, 安全な多能性幹細胞の選択方法

  山中 伸弥; 大貫 茉里

  
• 特表2014-523735, 特願2014-503890, 人工多能性幹細胞の選別方法

  山中 伸弥; 高橋 和利; 小柳 三千代; 大貫 茉里

  
• 特表2013-528356, 特願2012-556312, ヒト人工多能性幹細胞の選択方法

  山中 伸弥; 高橋 和利; 大貫 茉里

  
• 特表2013-503603, 特願2012-512108, 安全な多能性幹細胞の選択方法

  山中 伸弥; 大貫 茉里

  

External funds: Kakenhi

• 幹細胞の運命決定を担う霊長類特異的転移因子探索のための網羅的解析技術の確立

  Grant-in-Aid for Research Activity Start-up

  0701:Biology at molecular to cellular levels, and related fields

  Kyoto University

  大貫 茉里

  From 31 Aug. 2023, To 31 Mar. 2025, Project Closed

  転移因子;霊長類;多能性幹細胞;進化;内在性レトロウイルス;CRISPRスクリーニング;CRISPR-Cas13;転写制御

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K19348/
• ヒト特性に関わるノンコーディング変異の網羅的同定

  Grant-in-Aid for Scientific Research (B)

  Basic Section 43050:Genome biology-related

  Kyoto University

  井上 詞貴

  From 01 Apr. 2024, To 31 Mar. 2027, Granted

  エンハンサー;遺伝子発現制御;MPRA;進化;軟骨細胞;神経前駆細胞;ゲノム進化;ヒト進化

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K02004/

Education

Teaching subject(s)

• From 01 Apr. 2025, To 31 Mar. 2026

  ILAS Seminar

  Z001, Spring, Institute for Liberal Arts and Sciences, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  ILAS Seminar

  Z001, Spring, Institute for Liberal Arts and Sciences, 2