Basic Information

Professional Memberships

• From Apr. 2001, To Present

  THE JAPANESE SOCIETY FOR NEUTRON SCIENCE
• 日本生物物理学会
• From Nov. 1999, To Present

  THE CRYSTALLOGRAPHIC SOCIETY OF JAPAN

Academic Degree

• 修士（理学）(東京工業大学)
• 博士（理学）(東京工業大学)

Research History

• From Apr. 2018, To Present

  Kyoto University, 複合原子力科学研究所, 准教授
• From Apr. 2008, To Mar. 2018

  Kyoto University, Research Reactor Institute, 准教授
• From Aug. 2006, To Mar. 2008

  Kyoto University, Research Reactor Institute, 助教授
• From Apr. 2004, To Jul. 2006

  Chiba Institute of Science, Faculty of Pharmacy, 助教授
• From Apr. 2002, To Mar. 2004

  日本原子力研究所, 開放的融合研究推進員
• From Apr. 1999, To Mar. 2002

  日本原子力研究所, 博士研究員

Language of Instruction

• English
• Japanese

ID,URL

• J-Global ID

  201401091254225693

researchmap URL

• https://researchmap.jp/7000008550

Research

Research Topics, Overview of the research

• Research Topics

  Structural study of biomolecules using neutron and X-ray

• Overview of the research

  In order to understand an organism as an assembly of molecular machines, structures and functions of nucleic acids and proteins, which were parts of the molecular machines, are investigated by neutron and X-ray diffraction techniques.

Research Interests

• Protein structure
• hydration
• natto
• nucleic acid structure
• biophysics
• neutron crystallography

Research Areas

• Natural sciences, Bio-, chemical, and soft-matter physics
• Life sciences, Biophysics
• Energy, Quantum beam science, 中性子回折

Papers

• Neutron nucleic acid crystallography

  Chatake, T.

  Methods in Molecular Biology, 2016

  
• Site‐specific relaxation of peptide bond planarity induced by electrically attracted proton/deuteron observed by neutron crystallography

  Kaori Chiba; Takuro Matsui; Toshiyuki Chatake; Takashi Ohhara; Ichiro Tanaka; Katsuhide Yutani; Nobuo Niimura

  Protein Science, 25 Aug. 2023, Peer-reviewed

  
• Characterization of K-binding factor involved in water-soluble complex of menaquinone-7 produced by Bacillus subtilis natto

  Toshiyuki Chatake; Yasuhide Yanagisawa; Risa Murakami; Tadanori Ohsugi; Hiroyuki Sumi; Takumi Takata; Aya Okuda; Ken Morishima; Rintaro Inoue; Masaaki Sugiyama

  The Journal of Biochemistry, 07 Jul. 2023, Peer-reviewed, Lead author, Corresponding author

  
• X-ray analyses of d(GCGAAAGC) and d(GXGAAAGCT), where X = 2'-deoxy-5-iodocytidine.

  Sunami, T.; Kondo, J.; Chatake, T.; Hirao, I.; Watanabe, K.; Miura, K.; Tak{\'e}naka, A.

  Nucleic acids research. Supplement (2001), Nov. 2001

  
• Protonation states of hen egg-white lysozyme observed using D/H contrast neutron crystallography

  Toshiyuki Chatake; Ichiro Tanaka; Katsuhiro Kusaka; Satoru Fujiwara

  Acta Crystallographica Section D Structural Biology, 01 Jun. 2022, Peer-reviewed, Lead author, Corresponding author

  
• Evaluation of Radiation Damage to LAN-cable Sheaths in a Vacuum Vessel for Neutron Scattering

  T. Tominaga; M. Ueda; Y. Kawakita; T. Chatake; T. Saito; N. Sato

  The Japan Society for Neutron Science Hamon, May 2022, Peer-reviewed

  
• Recent structural insights into the mechanism of lysozyme hydrolysis

  Ichiro Tanaka; Ryota Nishinomiya; Ryosuke Goto; Shun Shimazaki; Toshiyuki Chatake

  Acta Crystallographica Section D Structural Biology, 01 Mar. 2021, Peer-reviewed

  Access this paper :

  
• Current status and near future plan of neutron protein crystallography at J-PARC

  Tanaka, I.; Chatake, T.; Fujiwara, S.; Hosoya, T.; Kusaka, K.; Niimura, N.; Yamada, T.; Yano, N.

  Methods in Enzymology, Feb. 2020, Invited

  
• Biophysical study of Bacillus subtilis natto : Physiologically active substances produced by Bacillus subtilis natto, and radioresistance of Bacillus subtilis natto

  T. Chatake; T. Saito; Y. Yanagisawa

  Radiation Biology Research Communications, Dec. 2018, Peer-reviewed, Lead author, Corresponding author

  
• Purification and structural characterization of water-soluble menaquinone-7 produced by Bacillus subtilis natto

  Toshiyuki Chatake; Yasuhide Yanagisawa; Rintaro Inoue; Mastaki Sugiyama; Tatsuhito Matsuo; Satoru Fujiwara; Tadanori Ohsugi; Hiroyuki Sumi

  Journal of food biochemistry, Jun. 2018, Peer-reviewed, Lead author, Corresponding author

  
• Cryoprotectant-free high-pressure cooling and dynamic nuclear polarization for more sensitive detection of hydrogen in neutron protein crystallography

  Tanaka, I.; Komatsuzaki, N.; Yue, W.-X.; Chatake, T.; Kusaka, K.; Niimura, N.; Miura, D.; Iwata, T.; Miyachi, Y.; Nukazuka, G.; Matsuda, H.

  Acta Crystallographica Section D: Structural Biology, Jan. 2018, Peer-reviewed

  
• Ligation-Dependent Picosecond Dynamics in Human Hemoglobin As Revealed by Quasielastic Neutron Scattering

  Satoru Fujiwara; Toshiyuki Chatake; Tatsuhito Matsuo; Fumiaki Kono; Taiki Tominaga; Kaoru Shibata; Ayana Sato-Tomita; Naoya Shibayama

  JOURNAL OF PHYSICAL CHEMISTRY B, Aug. 2017, Peer-reviewed

  
• DNA conformational transitions inferred from re-evaluation of m vertical bar F-o vertical bar - D vertical bar F-c vertical bar electron-density maps

  Tomoko Sunami; Toshiyuki Chatake; Hidetoshi Kono

  ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, Jul. 2017, Peer-reviewed

  
• ナットウキナーゼとメナキノン－７の構造研究

  柳澤泰任; 太田さくら; 足立達美; 内藤佐和; 須見洋行; 齊藤剛; 茶竹俊行

  テンペ研究会誌, 2017

  
• Structural Insight Into Protein Binding of Boron Tracedrug UTX-97 Revealed by the Co-Crystal Structure With Lysozyme at 1.26 angstrom Resolution

  Yukio Morimoto; Hideko Nagasawa; Yoshihiro Uto; Toshiyuki Chatake; Hitoshi Hori

  JOURNAL OF PHARMACEUTICAL SCIENCES, Aug. 2016, Peer-reviewed

  
• A technique for determining the deuterium/hydrogen contrast map in neutron macromolecular crystallography

  Toshiyuki Chatake; Satoru Fujiwara

  ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, Jan. 2016, Peer-reviewed, Lead author, Corresponding author

  
• Site specific oxidation of amino acid residues in rat lens gamma-crystallin induced by low-dose gamma-irradiation

  Ingu Kim; Takeshi Saito; Norihiko Fujii; Takashi Kanamoto; Toshiyuki Chatake; Noriko Fujii

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Oct. 2015, Peer-reviewed

  
• ATP binding and hydration state analyses of DAPK: Step toward neutron protein crystallography studies

  Yamaguchi A; Niimura N; Nakamura S; Kidokoro S; Chatake T; Yokoyama T; Tanaka I

  JPS Conf. Proc., 30 Mar. 2015, Peer-reviewed

  
• Fundamental studies for the proton polarization technique in neutron protein crystallography

  Ichiro Tanaka; Katsuhiro Kusaka; Toshiyuki Chatake; Nobuo Niimura

  JOURNAL OF SYNCHROTRON RADIATION, Nov. 2013, Peer-reviewed

  
• X-ray structure determination and deuteration of nattokinase

  Yasuhide Yanagisawa; Toshiyuki Chatake; Sawa Naito; Tadanori Ohsugi; Chieko Yatagai; Hiroyuki Sumi; Akio Kawaguchi; Kaori Chiba-Kamosida; Megumi Ogawa; Tatsumi Adachi; Yukio Morimoto

  JOURNAL OF SYNCHROTRON RADIATION, Nov. 2013, Peer-reviewed, Corresponding author

  
• Structural fluctuation observed in Z-DNA d(CGCGCG)(2) in the absence of divalent metal cations and polyamines

  Toshiyuki Chatake

  JOURNAL OF SYNCHROTRON RADIATION, Nov. 2013, Peer-reviewed, Lead author, Last author, Corresponding author

  
• Direct interactions between Z-DNA and alkaline earth cations, discovered in the presence of high concentrations of MgCl2 and CaCl2

  Toshiyuki Chatake; Tomoko Sunami

  JOURNAL OF INORGANIC BIOCHEMISTRY, Jul. 2013, Peer-reviewed, Lead author, Corresponding author

  
• Biomacromolecular Neutron Crystallography:The Basic Principles of Neutron Protein Crystallography

  Chatake Toshiyuki; Morimoto Yukio

  hamon, 2013, Peer-reviewed, Invited, Lead author, Corresponding author

  
• High-resolution X-ray study of the effects of deuteration on crystal growth and the crystal structure of proteinase K

  Toshiyuki Chatake; Takuya Ishikawa; Yasuhide Yanagisawa; Taro Yamada; Ichiro Tanaka; Satoru Fujiwara; Yukio Morimoro

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Nov. 2011, Peer-reviewed, Lead author, Corresponding author

  
• Proton polarization technique for neutron protein crystallography (NPC)

  N.Niimura; T.Chatake; I.Tanaka; K.Kusaka

  Acta Cryst. A, Aug. 2011

  
• Purification, crystallization and preliminary X-ray diffraction experiment of nattokinase from Bacillus subtilis natto

  Yasuhide Yanagisawa; Toshiyuki Chatake; Kaori Chiba-Kamoshida; Sawa Naito; Tadanori Ohsugi; Hiroyuki Sumi; Ichiro Yasuda; Yukio Morimoto

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Dec. 2010, Peer-reviewed, Corresponding author

  
• Protonation states of histidine and other key residues in deoxy normal human adult hemoglobin by neutron protein crystallography

  Andrey Kovalevsky; Toshiyuki Chatake; Naoya Shibayama; Sam-Yong Park; Takuya Ishikawa; Marat Mustyakimov; S. Zoe Fisher; Paul Langan; Yukio Morimoto

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Nov. 2010, Peer-reviewed

  
• Direct Determination of Protonation States of Histidine Residues in a 2 angstrom Neutron Structure of Deoxy-Human Normal Adult Hemoglobin and Implications for the Bohr Effect

  Andrey Y. Kovalevsky; Toshiyuki Chatake; Naoya Shibayama; Sam-Yong Park; Takuya Ishikawa; Marat Mustyakimov; Zoe Fisher; Paul Langan; Yukio Morimoto

  JOURNAL OF MOLECULAR BIOLOGY, Apr. 2010, Peer-reviewed

  
• An Approach to DNA Crystallization Using the Thermal Reversible Process of DNA Duplexes

  Toshiyuki Chatake; Gen Sazaki; Tatsuhiko Kikkou; Satoru Fujiwara; Takuya Ishikawa; Osamu Matsumoto; Yukio Morimoto

  CRYSTAL GROWTH & DESIGN, Mar. 2010, Peer-reviewed, Lead author, Corresponding author

  
• Functional aberration of myofibrils by cardiomyopathy-causing mutations in the coiled-coil region of the troponin-core domain

  Fumiko Matsumoto; Kayo Maeda; Toshiyuki Chatake; Yuichiro Maeda; Satoru Fujiwara

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Apr. 2009, Peer-reviewed

  
• An abnormal pK(a) value of internal histidine of the insulin molecule revealed by neutron crystallographic analysis

  Takuya Ishikawa; Toshiyuki Chatake; Yukio Morimoto; Mitsuru Maeda; Kazuo Kurihara; Ichiro Tanaka; Nobuo Niimura

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Nov. 2008, Peer-reviewed

  
• Hydrogen bonds of DsrD protein revealed by neutron crystallography

  Toshiyuki Chatake; Yoshiki Higuchi; Nobuhiro Mizuno; Ichiro Tanaka; Nobuo Niimura; Yukio Morimoto

  JOURNAL OF SYNCHROTRON RADIATION, May 2008, Peer-reviewed, Lead author

  
• Preliminary time-of-flight neutron diffraction study of human deoxyhemoglobin

  A. Y. Kovalevsky; T. Chatake; N. Shibayama; S. -Y. Park; T. Ishikawa; M. Mustyakimov; S. Z. Fisher; P. Langan; Y. Morimoto

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Apr. 2008, Peer-reviewed

  
• A neutron crystallographic analysis of a cubic porcine insulin at pD 6.6

  Takuya Ishikawa; Toshiyuki Chatake; Yuki Ohnishi; Ichiro Tanaka; Kazuo Kurihara; Ryota Kuroki; Nobuo Niimura

  CHEMICAL PHYSICS, Apr. 2008, Peer-reviewed

  
• Development of hydrogen and hydration database for biomolecules (HHDB)

  Okazaki Nobuo; Ohhara Takashi; Umino Hisao; Chatake Toshiyuki; Kurihara Kazuo; Cachau Raul E; Blaber Michael; Niimura Nobuo; Kuroki Ryota

  ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2008, Peer-reviewed

  
• Protonation states of buried histidine residues in human deoxyhemoglobin revealed by neutron crystallography

  Toshiyuki Chatake; Naoya Shibayama; Sam-Yong Park; Kazuo Kurihara; Taro Tamada; Ichiro Tanaka; Nobuo Niimura; Ryota Kuroki; Yukio Morimoto

  JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Dec. 2007, Peer-reviewed, Lead author

  
• Recent results on hydrogen and hydration in biology studied by neutron macromolecular crystallography

  N Niimura; S Arai; K Kurihara; T Chatake; Tanaka, I; R Bau

  CELLULAR AND MOLECULAR LIFE SCIENCES, Feb. 2006, Peer-reviewed

  
• The hydration structure of a Z-DNA hexameric duplex determined by a neutron diffraction technique

  T Chatake; Tanaka, I; H Umino; S Arai; N Niimura

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Aug. 2005, Peer-reviewed, Lead author

  
• Complicated water orientations in the minor groove of the B-DNA decamer d(CCATTAATGG)(2) observed by neutron diffraction measurements

  S Arai; T Chatake; T Ohhara; K Kurihara; Tanaka, I; N Suzuki; Z Fujimoto; H Mizuno; N Niimura

  NUCLEIC ACIDS RESEARCH, 2005, Peer-reviewed

  
• Hydrogen and Hydration DataBase for Bio-Macromolecules (HHDB)

  Kuroki Ryota; Chatake Toshiyuki; Kurihara Kazuo; Ohhara Takashi; Umino Hisao; Cachau Raul E; Blaber Michael; Niimura Nobuo

  ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2005, Peer-reviewed

  
• A neutron crystallographic analysis of a rubredoxin mutant at 1.6 angstrom resolution

  T Chatake; K Kurihara; Tanaka, I; Tsyba, I; R Bau; FE Jenney; MWW Adams; N Niimura

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Aug. 2004, Peer-reviewed, Lead author

  
• Neutron crystallographic study on rubredoxin from Pyrococcus furiosus by BIX-3, a single-crystal diffractometer for biomacromolecules

  K Kurihara; L Tanaka; T Chatake; MWW Adams; FE Jenney; N Moiseeva; R Bau; N Niimura

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Aug. 2004, Peer-reviewed

  
• More rapid evaluation of biomacromolecular crystals for diffraction experiments

  SD Arai; T Chatake; N Suzuki; H Mizuno; N Niimura

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Jun. 2004, Peer-reviewed

  
• Hydration structures in proteins and neutron diffraction experiment on dissimilatory sulfite reductase D (DsrD)

  T Chatake; A Ostermann; K Kurihara; FG Parak; N Mizuno; G Voordouw; Y Higuchi; Tanaka, I; N Niimura

  JOURNAL OF SYNCHROTRON RADIATION, Jan. 2004, Peer-reviewed, Lead author

  
• Crystallization of a large single crystal of cubic insulin for neutron protein crystallography

  M Maeda; T Chatake; Tanaka, I; A Ostermann; N Niimura

  JOURNAL OF SYNCHROTRON RADIATION, Jan. 2004, Peer-reviewed

  
• Hydrogen and hydration in proteins

  N Niimura; T Chatake; K Kurihara; M Maeda

  CELL BIOCHEMISTRY AND BIOPHYSICS, 2004, Peer-reviewed

  
• Crystallization and preliminary neutron analysis of the dissimilatory sulfite reductase D (DsrD) protein from the sulfate-reducing bacterium Desulfovibrio vulgaris

  T Chatake; N Mizuno; G Voordouw; Y Higuchi; S Arai; Tanaka, I; N Niimura

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Dec. 2003, Peer-reviewed, Lead author

  
• Hydration in proteins observed by high-resolution neutron crystallography

  T Chatake; A Ostermann; K Kurihara; FG Parak; N Niimura

  PROTEINS-STRUCTURE FUNCTION AND GENETICS, Feb. 2003, Peer-reviewed, Lead author

  
• Neutron structural biology, complementarity between neutron and X-ray

  Niimura Nobuo; Chatake Toshiyuki

  hamon, 2003, Peer-reviewed

  
• High resolution neutron protein crystallography. Hydrogen and hydration in proteins

  N Niimura; T Chatake; A Ostermann; K Kurihara; Tanaka, I

  ZEITSCHRIFT FUR KRISTALLOGRAPHIE, 2003, Peer-reviewed

  
• A neutron crystallographic analysis of a rubredoxin mutant

  T Chatake; K Kurihara; Tanaka, I; MWW Adams; FE Jenney; Tsyba, I; R Bau; N Niimura

  APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING, Dec. 2002, Peer-reviewed, Lead author

  
• A high-performance neutron diffractometer for biological crystallography (BIX-3)

  Tanaka, I; K Kurihara; T Chatake; N Niimura

  JOURNAL OF APPLIED CRYSTALLOGRAPHY, Feb. 2002, Peer-reviewed

  
• Crystallization of a large single crystal of a B-DNA decamer for a neutron diffraction experiment by the phase-diagram technique

  S Arai; T Chatake; Y Minezaki; N Niimura

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Jan. 2002, Peer-reviewed

  
• 中性子回折計BIX-3が拓く新しい構造の世界

  新村 信雄; 田中 伊知朗; 栗原 和男; 茶竹 俊行; Ostermann Andreas; 大原 高志

  波紋, 2002

  
• Neutron diffraction study of hydrous phase G: Hydrogen in the lower mantle hydrous silicate, phase G

  A Suzuki; E Ohtani; T Kondo; T Kuribayashi; N Niimura; K Kurihara; T Chatake

  GEOPHYSICAL RESEARCH LETTERS, Oct. 2001, Peer-reviewed

  
• Crystallographic studies on damaged DNAsIV. N-4-methoxycytosine shows a second face for Watson-Crick base-pairing, leading to purine transition mutagenesis

  MT Hossain; T Sunami; M Tsunoda; T Hikima; T Chatake; Y Ueno; A Matsuda; A Takenaka

  NUCLEIC ACIDS RESEARCH, Oct. 2001, Peer-reviewed

  
• Crystallographic studies on damaged DNAs: III. N-4-methoxycytosine can form both Watson-Crick type and wobbled base pairs in a B-form duplex

  MT Hossain; T Chatake; T Hikima; M Tsunoda; T Sunami; Y Ueno; A Matsuda; A Takenaka

  JOURNAL OF BIOCHEMISTRY, Jul. 2001, Peer-reviewed

  
• X-ray analyses of two DNA dodecamers containing N4-methoxy-cytosine paired with adenine or guanine.

  M.T. Hossain; T. Hikima; M. Tsunoda; T. Chatake; Y. Ueno; A. Matsuda; A. Takénaka

  Nucleic acids symposium series, Jan. 2000

  
• Crystallographic studies on damaged DNAs. II. N-6-methoxyadenine can present two alternate faces for Watson-Crick base-pairing, leading to pyrimidine transition mutagenesis

  T Chatake; T Hikima; A Ono; Y Ueno; A Matsuda; A Takenaka

  JOURNAL OF MOLECULAR BIOLOGY, Dec. 1999, Peer-reviewed, Lead author

  
• Crystallographic studies on damaged DNAs. I. An N-6-methoxyadenine residue forms a Watson-Crick pair with a cytosine residue in a B-DNA duplex

  T Chatake; A Ono; Y Ueno; A Matsuda; A Takenaka

  JOURNAL OF MOLECULAR BIOLOGY, Dec. 1999, Peer-reviewed, Lead author

  
• Crystallization and preliminary analysis of a DNA dodecamer of d(CGCGmo(6)AATCCGCG) containing 2 '-deoxy-N-6-methoxyadenosine: change in crystal packing with different humidity

  T Chatake; T Sunami; A Ono; Y Ueno; A Matsuda; A Takenaka

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Apr. 1999, Peer-reviewed

  
• X-ray analyses of DNA dodecamers containing 2'-deoxy-N[6]-methoxyadenosine

  Toshiyuki Chatake

  Mar. 1999, Peer-reviewed, Lead author, Corresponding author

  
• X-ray analysis of a DNA dodecamer containing 2'-deoxy-N4-methoxycytidine.

  T. Hikima; M. T. Hossain; T. Chatake; Y. Ueno; A. Matsuda; A. Takénaka

  Nucleic acids symposium series, Jan. 1999

  
• N6-methoxyadenine in damaged DNA has two faces in property of Watson-Crick base pairing.

  T. Chatake; A. Ono; Y. Ueno; A. Matsuda; A. Takénaka

  Nucleic acids symposium series, 01 Jan. 1999, Lead author

  
• X-ray analysis of DNA dodecamer containing 2'-deoxy-N6-methoxyadenosine.

  Chatake, T.; Toyoda, T.; Tsunoda, M.; Sunami, T.; Ono, A.; Ueno, Y.; Matsuda, A.; Takenaka, A.

  Nucleic acids symposium series, 01 Jan. 1997, Lead author

  
• STRUCTURAL COMPOSITION OF HAMMERHEAD RIBOZYMES

  A TAKENAKA; O MATSUMOTO; YX CHEN; S HASEGAWA; T CHATAKE; M TSUNODA; T OHTA; Y KOMATSU; M KOIZUMI; E OHTSUKA

  JOURNAL OF BIOCHEMISTRY, Apr. 1995, Peer-reviewed

  

Misc.

• NAG4リゾチーム複合体のプロトネーションと反応機構について

  河部 雄太; 田中 伊知朗; 茶竹 俊行

  2024年度量子ビームサイエンスフェスタ予稿集, Mar. 2025

  
• リゾチーム酵素活性に及ぼすリガンドや水和水の影響

  栗林 拓実; 茶竹 俊行; 河部 雄太; 田中 伊知朗

  2024年度量子ビームサイエンスフェスタ予稿集, Mar. 2025

  
• ペプチド結合の平面性の部位特異的緩和

  千葉 かおり; 茶竹 俊行; 大原 高志

  2024年度量子ビームサイエンスフェスタ予稿集, Mar. 2025

  
• Crystallographic analysis of protein hydration by D/H contrast technique

  T. Sunami; T. Chatake; S. Fujiwara; I. Tanaka; K. Kusaka

  日本蛋白質科学会年会要旨集, Jun. 2024

  
• Structural study of water-soluble vitamin K from Bacillus subtilis natto

  T. Chatake; A. Okoda; K. Morishima; R. Inoue; M. Sugiyama; T. Takata; Y. Yanagisawa

  KURNS Progress Report 2023, Jul. 2024, Lead author, Corresponding author

  
• DNA Crystallization using temperature-control technique

  茶竹俊行; 佐崎元; 亀甲達彦; 藤原悟; 石川卓哉; 松本治; 森本幸生

  結晶成長国内会議予稿集, 2009

  
• Oral Fibrinolytic Therapy -Nattokinase in Health and Disease Prevention-

  須見 洋行; 柳澤 泰任; 茶竹 俊行; 満尾 正; 井上 浩義; 島谷 和弘; 内藤 佐和; 矢田貝 智恵子

  New food industry = ニューフードインダストリー, Jan. 2024

  
• Study of protein hydration

  Toshiyuki Chatake; Tomoko Sunami; Satoru Fujiwara; Ichiro Tanaka; Katsuhiro Kusaka

  京都大学複合原子力科学研究所学術講演会報文集, Feb. 2024

  
• Study of water-soluble vitamin K2 from Bacillus subtilis natto

  Toshiyuki Chatake; Yasuhide Yanagisawa; Takumi Takata; Aya Okuda; Ken Morishima; Rintaro Inoue; Masaaki Sugiyama

  京都大学複合原子力科学研究所学術講演会報文集, Jan. 2024, Lead author, Corresponding author

  
• Structural study of water-soluble complex of menaquinone-7 produced by Bacillus subtilis natto

  CHATAKE Toshiyuki; YANAGISAWA Yasuhide; MURAKAMI Risa; OHSUGI Tadanori; SUMI Hiroyuki; OKUDA Aya; MORISHIMA Ken; INOUE Rintaro; SUGIYAMA Masaaki

  生物物理(Web), 2021

  
• Analysis of water-soluble vitamin K complex from Bacillus subtilis natto

  T. Chatake; A. Okuda; K. Morishima; R. Inoue; M. Sugiyama; T. Takata; Y. Yanagisawa

  KURNS Progress Report 2022, Jul. 2023, Lead author, Corresponding author

  
• Measurement of the resistance of the spores of Bacillus subtilis natto

  T. ChatakeT; SaitoY. Yanagisawa

  KURNS Progress Report 2022, Jul. 2023, Lead author, Corresponding author

  
• Neutron crystallographic approach to reveal hydration structure of protein

  Toshiyuki Chatake; Ichiro Tanaka; Katsuhiro Kusaka; Tomoko Sunami; Satoru Fujiwara

  Proceedings of the 57th KURNS Scientific Meeting, Feb. 2023, Lead author, Corresponding author

  
• Structural analysis of water-soluble vitamin K2 derived from Bacillus subtilis natto

  T. Chatake; Y. Yanagisawa; A. Okuda; K. Morishima; R. Inoue; M. Sugiyama

  Proceedings of the 56th KURNS Scientific Meeting, Feb. 2022, Lead author, Corresponding author

  
• Evaluation of LAN cable sheath degradation by;gamma irradiation

  Taiki Tominaga; Toshiyuki Chatake; Takeshi Saito; Nobuhiro Sato; Rintaro Inoue; Masaaki Sugiyama

  KURNS Progress Report 2020, Jul. 2021

  
• Preliminary analysis of structural composition of menaquinone-7 Bacillus subtilis natto

  T. Chatake, Y. Yanagiswa, A. Okuda, K. Morishima, R. Inoue, M. Sugiyama

  KURNS Progress Report 2019, Jun. 2020, Lead author, Corresponding author

  
• Fibrinolysis and disease prevention effects of nattokinase

  須見 洋行; 矢田貝 智恵子; 茶竹 俊行; 森本 幸生; 柳澤 泰任; 満尾 正; 井上 浩義; 丸山 眞杉

  Food style 21, Nov. 2018

  
• Biophysical studies of Bacillus subtilis natto

  Y. Yanagisawa; T. Chatake; T. Saito; K. Morishima, R; Inoue; M. Sugiyama

  KURRI PROGRESS REPORT 2017, Jun. 2018

  
• Start-up of SEC-SAXS system

  R. Inoue; T. Chatake; N. Sato; K. Morishima; M. Sugiyama

  京都大学原子炉実験所学術講演会報文集, Jan. 2018

  
• Radiation resistance with gamma rays of Bacillus subtilis natto in a logarithmic growth phase

  T. Chatake; T. Saito; Y. Yanagisawa

  京都大学原子炉実験所学術講演会報文集, Jan. 2018, Lead author

  
• Purification, crystallization, and x-ray diffraction study of lysozyme in D2O and H2O solution

  T. Chatake; S. Fujiwara; I. Tanaka

  KURRI PROGRESS REPORT 2016, Jul. 2017, Lead author, Corresponding author

  
• Preliminary study of radiation resistivity of Bacillus subtilis natto

  Y. Yanagisawa; T. Chatake; T. Saito; T. Ohsugi; H. Sumi

  KURRI PROGRESS REPORT 2016, Jul. 2017

  
• Structural properties of MK-7 and gamma-ray resistance of Bacillus subtilis natto

  Y. Yanagisawa, A. Takane, T. Adachi, T. Chatake, T Saito, R. Inoue, M. Sugiyama, T. Matsuo, S. Fujiwara, T. Ohsugi, H. Sumi

  京都大学原子炉実験所学術講演会報文集, Jan. 2017

  
• D/H contrast method in neutron protein crystallography toward revealing hydration structures of macromolecules

  T. Chatake; S. Fujiwara

  京都大学原子炉実験所学術講演会報文集, Jan. 2017, Lead author, Corresponding author

  
• Development of Experimental Techniques for Neutron Biology

  T. Chatake; S. Fujiwara; Y. Yanagisawa; I. Tanaka

  KURRI PROGRESS REPORT 2015, Jul. 2016, Lead author, Corresponding author

  
• D/H contrast neutron protein crystallography

  T. Chatake; S. Fujiwara

  京都大学原子炉実験所学術講演会報文集, Jan. 2016, Lead author, Corresponding author

  
• Protein solution structural analysis assisted by deuterated protein

  R. Inoue; T. Chatake; Y. Yanagisawa; E. Chatani; M. Hoshino; N. Fujii

  京都大学原子炉実験所学術講演会報文集, Jan. 2016

  
• DNA分光器における生体関連物質の非弾性・準弾性散乱研究の現状と今後の計画

  柴田薫; 川北至信; 高橋伸明; 藤原悟; 松尾龍人; 河野史明; 茶竹俊行; 中川洋; 富永大輝

  日本中性子科学会年会講演概要集, 2016

  
• Development of improved techniques for neutron biology

  T. Chatake; S. Fujiwara; I. Tanaka; Y. Yanagisawa

  KURRI PROGRESS REPORT 2014, Jun. 2015, Lead author, Corresponding author

  
• Structural studies on proton/protonation of the protein molecule.

  森本幸生; 山口宏; 細川桂一; 村上琢人; 喜田昭子; 海野昌喜; 久留一郎; 茶竹俊行; 柳澤泰任; 藤原悟; 田中伊智朗; 日高雄二; 島本茂; 中西健

  京都大学原子炉実験所学術講演会報文集, Jan. 2015

  
• Particular DNA structures

  Toshiyuki Chatake

  Crystallography in Japan (II), Jul. 2014, Peer-reviewed, Invited, Lead author

  
• Preparation of nattokinase and menaquinone-7 for neutron experiment

  Y. Yanagisawa; M. Sakanushi; M. ishiyama; S. Onuma; S. Fujiwara; T. Chatake; Y. Morimoto

  KURRI PROGRESS REPORT 2013, Jun. 2014

  
• Crystallographic study of hydration and metal interactions of DNA

  T. Chatake; S. Fujiwara; Y. Morimoto

  KURRI PROGRESS REPORT 2013, Jun. 2014, Lead author, Corresponding author

  
• Structural study of water-soluble vitamin K2 from Bacillus subtilis natto

  T. Chatake; Y. Yanagisawa; T. Matsuo; S. Fujiwara; Y. Morimoto

  Proceedings of the 48th KURRI Scientific Meeting, Jan. 2014, Lead author

  
• Production of deuterated nattokinase from D2O resistance strain of Bacillus subtilis natto

  T. Chatake; Y. Yanagisawa; Y. Morimoto

  KURRI PROGRESS REPORT 2012, Jun. 2013, Lead author, Corresponding author

  
• Time of flight neutron experiment of Z-DNA hexamer d(CGCGCG)

  T. Chatake; S. Fujiwara; Y. Morimoto

  KURRI PROGRESS REPORT 2012, Jun. 2013, Lead author, Corresponding author

  
• 3P286 Fundamental trials for proton polarization technique in neutron protein crystallography(26. Measurements,Poster)

  Ichiro Tanaka; Katsuhiro Kusaka; Toshiyuki Chatake; Nobuo Niimura

  Seibutsu Butsuri, 2013

  
• 生体高分子の機能発現に重要な役割をもつ水分子およびプロトン化状態の直接観測

  樋口芳樹; 茶竹俊行; 横山武司; 山田太郎

  2012年第2回水和ナノ構造研究会, 2013

  
• Measurements of neutron transmissions of biomacromolecular solutions using 4CND

  T. Chatake; S. Fujiwara; A. Kawaguchi; T. Matsuo; Y. Morimoto

  KURRI PROGRESS REPORT 2011, Jun. 2012, Lead author, Corresponding author

  
• Cultivation of Bacillus subtilis natto in deuterated medium

  T. Chatake; Y. Morimoto; M. Ogawa; Y. Yanagisawa

  KURRI PROGRESS REPORT 2011, Jun. 2012, Lead author, Corresponding author

  
• Approach to a Deuterium Technique for Biological Macromolecules : Open for the KURDshop

  森本 幸生; 杉山 正明; 茶竹 俊行

  京都大学原子炉実験所学術講演会報文集, Jan. 2012

  
• 高塩状態で誘導されるDNA会合の研究

  茶竹俊行

  タンパク質の異常凝集とその防御?修復機構に関する研究会, 2012

  
• 生体試料解析のための重水素化手法の確立

  森本幸生; 杉山正明; 茶竹俊行; 藤井紀子; 斎藤毅; 藤原悟; 日高雄二

  京都大学原子炉実験所第46回学術講演会, 2012

  
• Time of Flight Neutron Crystallographic analysis of Human Carbonmonoxyhemoglobin

  Chatake, T; Shibayama, N; Park, S.Y; Tomoyori, K; Hosoya, T; Ohara, T; Kusaka, K; Kurihara, K; Tanaka, I; Niimura, N; Morimoto, Y

  1st Asia-Oceania Conference on Neutron Scattering, 23 Nov. 2011

  
• High-resolution X-ray crystallographic investigation of the effect of deuteration of crystallization solution

  T. Chatake; S. Fujiwara; T. Ishikawa; Y. Yanagisawa; T. Yamada; I. Tanaka; Y. Morimoto

  KURRI PROGRESS REPORT 2010, Jun. 2011, Lead author, Corresponding author

  
• Structure determination of nattokinase from Bacillus subtilis natto

  Y. Yanagisawa; T. Chatake; K. Chiba-Kamosida; S. Naito; T. Ohsugi; H. Sumi; I. Yasuda; Y. Morimoto

  KURRI PROGRESS REPORT 2010, Jun. 2011

  
• Book Review

  CHATAKE Toshiyuki

  Nihon Kessho Gakkaishi, 2011

  
• Crystallographic study of nattokinase from Bacillus subtilis natto

  柳澤 泰任; 茶竹 俊行; 千葉 かおり

  京都大学原子炉実験所学術講演会報文集, Jan. 2011

  
• DNAの水和構造における水分子と金属イオン

  茶竹俊行

  第一回バイオ単分子?水和ナノ構造合同研究会, 2011

  
• 新刊紹介「Neutron Protein Crystallography」

  茶竹俊行

  日本結晶学会誌, 2011

  
• Crystallization of ribonuclease A and H/D exchange for neutron diffraction experiment

  T. Chatake; S. Fujiwara; Y. Morimoto

  KURRI PROGRESS REPORT 2009, Jun. 2010, Lead author, Corresponding author

  
• Large scale preparation of bio-macromolecules with Jar-fermentor system to deuterium treatments

  T. Chatake; T. Saito; N. Fujii; Y. Morimoto

  KURRI PROGRESS REPORT 2009, Jun. 2010, Lead author

  
• Purification and crystallization of nattokinase from Bacillus subtilis natto

  Y. Yanagisawa; T. Chatake; T. Ishikawa; Y. Morimoto; I. Yasuda; T. Ohsugi; H. Sumi

  KURRI PROGRESS REPORT 2009, Jun. 2010

  
• Study of effect of deuteration on crystal structure of proteinase K by high resolution X-ray diffraction technique

  T. Ishikawa; T. Chatake; A. Kawaguchi; Y. Yanagisawa; S. Fujiwata; Y. Morimoto

  KURRI PROGRESS REPORT 2009, Jun. 2010

  
• 中性子捕捉療法におけるカルボラン薬剤・生体分子複合体の細胞内挙動と分子生物学的研究

  茶竹 俊行

  医科学応用研究財団研究報告, 2010

  
• 1SH1015 Neutron Crystallography in Structural Biology(1SH A New Break of Dawn of "Neutrons in Biology"-New Prospects of Neutron Biophysics with J-PARC-,The 48th Annual Meeting of the Biophysical Society of Japan)

  Chatake Toshiyuki

  Seibutsu Butsuri, 2010

  
• 納豆菌由来メナキノン-7(MK-7)の結晶化

  柳澤泰任; 中里祐; 内田麻友; 染谷智恵; 足立達美; 安田一郎; 大杉忠則; 須見洋行; 茶竹俊行; 森本幸生

  京都大学原子炉実験所学術講演会報文集, 2009

  
• 納豆菌培養液由来ナットウキナーゼの精製と結晶化

  柳澤泰任; 茶竹俊行; 大杉忠則; 千葉かおり; 安田一郎; 森本幸生; 須見洋行

  日本結晶学会年会講演要旨集, 2009

  
• 3SA4-03 Neutron crystallographic analysis of human deoxyhemoglobin(3SA4 Hemoglobin revisited,The 47th Annual Meeting of the Biophysical Society of Japan)

  Chatake Toshiyuki; Morimoto Yukio

  Seibutsu Butsuri, 2009

  
• Neutron crystallographic analysis of human deoxyhemoglobin

  Toshiyuki Chatake; Yukio Morimoto

  日本生物物理学会2009年会マイクロシンポジウム, 2009, Lead author, Last author, Corresponding author

  
• 温度制御によるDNAの結晶化とその応用

  茶竹俊行

  結晶成長ワークショップ, 2009

  
• Preliminary expriment for neutron diffraction study of proteinase K

  T. Chatake; T. Ishikawa; A. Kawaguchi; Y. Morimoto; Y. Yanagisawa; I. Tanaka

  KURRI Progress Report 2007, 2008

  
• Neutron experiment of human deoxyhemoglobin using PCS at LANSCE

  茶竹 俊行; 石川 卓哉; Kovalevskyi Andrii Y

  京都大学原子炉実験所学術講演会報文集, Jan. 2008

  
• The Effect of Deuterium Oxide on Hydration Structure of Proteinase K

  T. Ishikawa; T. Chatake; Y. Morimoto

  XXI Congress of the Int. Union of Crystallography, 2008

  
• Crystal Structure of Z-DNA d(CGCGCG) Complexed with Ca2+ Ion 、 and Mg2+ Ion

  T. Chatake; T. Ishikawa; Y. Morimoto

  XXI Congress of the Int. Union of Crystallography, 2008

  
• Time-of-Flight Neutron Diffraction Study of Human Deoxyhemoglobin

  A. Y. Kovalevsky; T. Chatake; T. Ishikawa; N. Shibayama; S.-Y. Park; M. Mustyakimov; P. Langan; Y. Morimoto

  ACA 2008 (Meeting of the American Crystallographic Association), 2008

  
• Protocol of Refinement in Neutron Crystallographic Analysis

  Chatake Toshiyuki; Morimoto Yukio

  hamon, 2007

  
• Neutron Structural Biology of Nucleic Acids and Proteins

  T. Chatake

  169th Commitee on Structural Biology using Diffraction Techniques、24th meeting、 Oct 25th, 2007

  
• 生体分子の水素・水和構造と中性子結晶解析 (第41回京都大学原子炉実験所学術講演会 報文集)

  茶竹 俊行; 森本 幸生

  京都大学原子炉実験所学術講演会報文集, Jan. 2007

  
• 中性子解析による蛋白質の水素・水和構造の精密化 (第41回京都大学原子炉実験所学術講演会 報文集)

  茶竹 俊行; 森本 幸生

  京都大学原子炉実験所学術講演会報文集, Jan. 2007

  
• Crystallization of Proteinase K in the Deuterated Solution for Neutron Analysis

  T. Chatake; K. Kurihara; R. Kuroki; Y. Morimoto

  International Symposium on Diffraction Structural Biology, 2007

  
• Crystallization of DNA oligomers by a simple temperature-control technique

  T. Chatake; O. Matsumoto; T. Kikkou

  Joint conference of the Asian Crystallographic Association and the Crystallographic Society of Japan\n, 21 Nov. 2006

  
• Orientations of Water Molecules Surrounding B-DNA Observed by Neutron Crystallographic Analysis

  ARAI Shigeki; CHATAKE Toshiyuki; NIIMURA Nobuo

  Nihon Kessho Gakkaishi, 28 Apr. 2006

  
• Z-DNA6量体の中性子結晶解析

  茶竹俊行; 田中伊知朗; 海野久雄; 新井栄揮; 新村信雄

  日本中性子科学会年会講演概要集, 2006

  
• 異なる水素イオン濃度における立方晶インスリンの中性子構造解析

  石川卓哉; 田中伊知朗; 茶竹俊行; 栗原和男; 大西裕季; 日下勝弘; 玉田太郎; 黒木良太; 新村信雄

  日本中性子科学会年会講演概要集, 2006

  
• DNAの水素と水和構造

  茶竹 俊行; 新井 栄揮; 新村 信雄

  現代化学, Oct. 2005, Invited

  
• Hydrogen and Hydration in Proteins and Nucleic Acids. Neutron Diffraction from Bio-macromolecules at JAERI

  Nobuo Niimura; Shigeki Arai; Kazuo Kurihara; Toshiyuki Chatake; Ichiro Tanaka; Robert Bau

  Hydrogen- and Hydration-Sensitive Structural Biology, Jul. 2005, Invited

  
• Neutron and X-ray Crystallographic Analysis of DNA Oligomers. Hydrogen and Hydration in DNA Molecules

  CHATAKE Toshiyuki

  Nihon Kessho Gakkaishi, 30 Jun. 2005, Invited, Lead author, Corresponding author

  
• クリスタリット

  後藤 俊治; 茶竹 俊行; 水島 恒裕; 西堀 英治; 田中 信忠; 池田 卓史

  日本結晶学会誌, 2005

  
• Neutron Diffraction Experiment of DsrD protein

  T. Chatake; N. Mizuno; G. Voordouw; Y. Higuchi; I. Tanaka; N. Niimura

  JAERI-Review, 2004

  
• 3P035 Site-specific twist of peptide bond in human lysozyme by localized hydrogen or hydration water observed by neutron crystallography

  Chiba K.; Matsui T.; Chatake T.; Ohhara T.; Ostermann Andreas; Tanaka I.; Yutani K.; Niimura N.

  Seibutsu Butsuri, 2004

  
• ”Complicated Water Network in the Minor Groove of B-DNA Decamer\nd(CCATTAATGG)2 observed by Neutron Diffraction Measurements”

  S. Arai; T. Chatake; T. Ohhara; K. Kurihara; I. Tanaka; N. Suzuki; Z. Fujimoto; H. Mizuno; N. Niimura

  The 5th International Conference on Biological Physics\n, 2004

  
• タンパク質の水素・水和構造

  新村信雄; 茶竹俊行; 栗原和男

  酵素工学ニュース\n, 2003

  
• Neutron crystallography of B-DNA decamer d(CCATTAATGG)

  Arai S.; Chatake T.; Kurihara K.; Tanaka I.; Ohhara T.; Niimura N.

  Seibutsu Butsuri, 2003

  
• Neutron crystallographic analysis of DsrD protein

  Chatake T.; Mizuo N.; Higuchi Y.; Voordouw Gerrit; Tanaka I.; Niimura N.

  Seibutsu Butsuri, 2003

  
• Neutron protein crystallography of insulin

  Maeda M.; Chatake T.; Tanaka I.; Kurihara K.; Niimura N.

  Seibutsu Butsuri, 2003

  
• ”Crystallization Process of HEWL and Rational Crystallization Design of aLarge Single Crystal for Neutron Protein Crystallography\n \n ”

  N.Niimura M.Ataka; S.Arai; M.Maeda; T.Chatake

  The 9th International Conference. on the Crystallization of Biological Macromolecules\n, 2002

  
• 中性子結晶学による生体分子の水素・水和構造の解析

  茶竹俊行

  結晶化学研究会第52回講演会, 2002

  
• タンパク質中性子回折結果実例紹介

  茶竹俊行

  第219回CBI学会研究講演会, 2002

  
• Two Alternate Faces of Methoxylated Cytosine Residues for Pairing with G and A in the Watson-Crick Geometry、 Leading to Purine transition Mutagenesis”

  M. T. Hossain; T. Hikima; T. Chatake; M. Tsunoda; Y. Ueno; A. Matsuda; A. Takenaka

  The 4th Asian Crystallographic Association Meeting.\n, 18 Nov. 2001

  
• 中性子構造生物学の現状

  新村信雄; 田中伊知朗; 栗原和男; 茶竹俊行; Andreas Ostermann

  構造生物\n, 2001

  
• 中性子回折計BIX-3によるこの1年の成果

  田中伊知朗; 栗原和男; 茶竹俊行; 新井栄樹; OSTERMANN A; 大原高志; 千葉薫; 新村信雄

  日本中性子科学会年会講演概要集, 2001

  
• Neutron Diffractometer for Biological Crystallography (BIX-3)

  I.Tanaka; K.Kurihara; T.Chatake; N.Niimura

  J. Phys. Soc. Jpn., 2001

  
• Study on the crystal growth of cytochrome c'

  Watanabe H; Kohzuma T; Nakamura H; Yoda S; Yamanaka A; Ostermann Andreas; Kurihara K; Chatake T; Tanaka I; Niimura N

  Seibutsu Butsuri, 2001

  
• A new single crystal diffractometer for neutron protein crystallography (BIX-3M)

  K.Kurihara; I.Tanaka; T.Chatake; A.Ostermann; N.Niimura

  The International Conference on Neutron Scattering 2001\n, 2001

  
• Neutron diffraction studies on thermostability of rubredoxin.

  R. Bau; N. Moiseva; I.Tsyba; N.Niimura; T.Chatake; K.Kurihara; MWW. Adams; FE. Jenney

  The International Conference on Neutron Scattering 2001\n, 2001

  
• Crystal structures of DNAs damaged by methoxylation、 and pyrimidine- and purine-transitionmutagenesis

  A. Takenaka; M. T. Hossain; T. Chatake; T. Hikima; A. Ono; Y. Ueno; A. Matsuda

  The 4th International Chemical Congress of Pacific Basin Societies\n, 12 Dec. 2000

  
• DNA中性子結晶解析遺伝情報に関わる水和ネットワーク解明を目指して

  茶竹 俊行

  日本結晶学会誌, 2000

  
• Phase diagram for B-DNA crystallization systems and its application

  Arai S.; Chatake T.; Minezaki Y.; Niimura N.

  Seibutsu Butsuri, 2000

  
• Crystal structures of DNAs damaged by methoxylation reveal the reason why pyrimidine transition and purine transition occur during replication

  A. Takenaka; T. Chatake; T. Hikima; M. T. Hossain; A. Ono; Y. Ueno; A. Matsuda

  The 19th European Crystallographic Meeting\n, 2000

  
• 若手研究者が語る結晶学の夢.生体高分子セッション3

  茶竹俊行

  日本結晶学会創設50周年記念シンポジウム, 2000

  
• 中性子イメージングプレートを用いたphase Gの構造解析

  鈴木昭夫; 大谷栄治; 新村信雄; 栗原和男; 茶竹俊行; 近藤忠; 栗林貴弘

  日本岩石鉱物鉱床学会学術講演会講演要旨集, Sep. 1999

  
• Dynamic reaction mechanism of hammerhead ribozyme

  T. Chatake; M. Tsunoda; T. Hossain; T. Sunami; T. Hikima

  Photon Factory Activity Report Part B, 1999

  
• 安定なヘアピン構造を含むデオキシオリゴヌクレオチドのX線解析

  角南 智子; 茶竹 俊行; 平尾 一郎; 横山 茂之; 渡辺 公綱; 三浦 謹一郎; 竹中 章郎

  日本結晶学会誌, 1998

  
• X-ray analysis of DNA dodecamer containing N6-methoxyadenine

  T. Chatake; T. Toyoda; D. Tsuchiya; Y. Iga; M. Tsunoda; T. Sekiguchi; A. Ono; Y. Ueno; A. Matsuda; A. Takenaka

  Photon Factory Activity Report Part B, 1997, Lead author

  
• X-ray analysis of eucaryotic yeast lioamide dehydrogenase

  T. Toyoda; D. Tsuchiya; Y. Iga; T. Chatake; M. Tsunoda; T. Sekiguchi; A. Takenaka

  Photon Factory Activity Report Part B, 1997

  
• 2'-デオキシ-N6-メトキシアデノシンを含むDNA12量体結晶のパッキング変化

  茶竹 俊行; 角南 智子; 角田 大; 豊田 友彦; 小野 晶; 上野 義仁; 松田 彰; 竹中 章郎

  日本結晶学会誌, 1997

  
• X-ray analysis of 3-isopropylmalate dehydrogenase from the moderate, facultative thermophile Bacillus Coagulans

  D. Tsuchiya; M. Tsunoda; T. Chatake; Y. Iga; T. Toyoda; A. Takenaka

  Photon Factory Activity Report Part B, 1996

  
• X-ray structure of eukaryotic E4, lipoamide dehydrogenase, from yeast

  T. Toyoda; D. Tsuchiya; Y. Iga; T. Chatake; M. Tsunoda; A. Takenaka

  Photon Factory Activity Report Part B, 1996

  
• X-ray analysis of 3-isopropylmalate dehydrogenase from facultative thermaophile, Bacillus Coagulans

  D. Tsuchiya; T. Chatake; Y. Iga; Y. Chen; T. Toyoda; O. Matsumoto; A. Takenaka

  Photon Factory Activity Report Part B, 1995

  
• Crystallographic analysis of yeast lipoamide dehydrogenase

  T. Toyoda; D. Tsuchiya; T. Chatake; Y. Iga; O. Matsumoto; A. Takenaka

  Photon Factory Activity Report Part B, 1995

  
• Structural studies for time resolved reaction mechanism on ribonuclease

  Y. Iga; T. Chatake; M. Tsunoda; Y. Chen; D. Tsuchiya; T. Toyoda; O. Matsumoto; A. Takenaka

  Photon Factory Activity Report Part B, 1995

  
• Structural studies on hammer head ribozymes

  T. Chatake; M. Tsunoda; Y. Iga; Y. Chen; O. Matsumoto; D. Tsuchiya; T. Toyoda; A. Takenaka

  Photon Factory Activity Report Part B, 1995, Lead author

  

Presentations

• D/Hコントラスト中性子結晶解析による水和水の位置決定と検証

  茶竹俊行; 角南智子; 藤原悟; 田中伊知朗; 日下勝弘

  2024日本結晶学会年会, 08 Nov. 2024

  
• Protein hydration observed in D/H contrast neutron crystallography

  T. Chatake; T. Sunami; S. Fujiwara; I. Tanaka; K. Kusaka

  9th International Symposium of Quantum Beam Science, 19 Oct. 2024, Invited

  
• Three-dimensional structure of water-soluble vitamin K2 produced by Bacillus subtilis natto.

  Toshiyuki Chatake; Yasuhide Yanagisawa; Takumi Takata; Aya Okuda; Ken Morishima; Rintaro Inoue; Masaaki Sugiyama

  第15回 タンパク質の異常凝集とその防御・修復機構に関する研究会, 28 Aug. 2024

  
• 生体物質単結晶回折

  茶竹俊行

  中性子ビーム利用基礎講習会, 08 Dec. 2009, Invited

  
• Hydration of biomacromolecules: Protein and DNA

  Toshiyuki Chatake

  中性子構造研究会, 27 Jan. 2010

  
• 温度制御によるDNAの結晶化とその応用

  茶竹俊行

  蛋白質結晶成長研究会, 06 Mar. 2009, Invited

  
• Neutron analysis of protein hydration –solution and crystal -

  T. Chatake; I. Tanaka; K. Kusaka; T. Sunami; S. Fujiwara

  第14回 タンパク質の異常凝集とその防御・修復機構に関する研究会, 10 Mar. 2023

  
• D/H contrast technique for neutron crystallography

  T. Chatake; I. Tanaka; K. Kusaka; S. Fujiwara

  The 6th International Symposium of Quantum Beam Science at Ibaraki University, 22 Feb. 2022

  
• 実空間 D/H コントラスト法による中性子蛋白質結晶解析

  茶竹俊行; 藤原悟

  2017年度中性子科学会年会, 03 Dec. 2017

  
• 新たな解析法による中性子結晶解析からの水和構造へのアプローチ

  茶竹 俊行; 藤原悟

  第3回Neutron in Biology 研究会, Mar. 2015

  
• 核酸科学を中心とした中性子結晶構造解析

  第1回Neutron in Biology研究会, 14 Mar. 2013, Neutron in Biology研究会

  
• Neutron Crystallography in Structural Biology

  日本生物物理学会2010年会マイクロシンポジウム, 20 Sep. 2010, 日本生物物理学会

  
• 中性子結晶解析による生体高分子の機能性プロトンの研究

  日本中性子科学会2010年会

  
• 高塩濃度下で形成されるDNA と二価金属の直接的相互作用

  日本結晶学会2010年会

  
• 生体プロトン解析用中性子回折計の計画

  日本中性子科学会2009年会

  
• 納豆菌培養液由来ナットウキナーゼの精製と結晶化

  日本結晶学会2009年会

  
• 温度制御によるDNAの結晶化

  日本結晶成長学会2009年会

  
• 核酸・蛋白質の中性子構造生物学による展開

  回折構造生物第169委員会 第24回研究会

  
• マクロシーディング法による大型結晶作成

  平成18年度 第２回 新薬創生研究会

  
• Hydration in proteins and DNA

  「新薬創生研究会」国際フォーラム

  
• X線及び中性子線結晶学によるDNAの水素および水和の構造生物学的研究.

  日本結晶学会平成16年度年会

  
• Neutron protein crystallography. Hydrogen and hydration in proteins.

  The 3rd Japan- Korea meeting on neutron science

  

Books and Other Publications

• Crystallography in Japan (II)

  Toshiyuki Chatake, Contributor, 第4章 構造化学・材料化学, 1. 構造化学, DNAの特異な構造, pp.218-219

  日本結晶学会, Jul. 2014

  Find Kyoto University's collection of books :

  
• 生体高分子結晶のX線構造解析 : 実践手法と基礎理論

  竹中, 章郎; 熊坂, 崇; 近藤, 次郎; 角南, 智子; 茶竹, 俊行; 森下, えら, Contributor, 5,6,7章を茶竹と竹中の共著 (pp.90-173)

  丸善出版, Feb. 2022

  Find Kyoto University's collection of books :

  
• Neutron nucleic acid crystallography in Methods Molecular biology 1320, 283, 300

  Toshiyuki Chatake, Contributor, Neutron nucleic acid crystallography pp. 283-300

  Springer, Aug. 2015, Not refereed

  
• 「製剤への物理化学 第2版」(第三章を松本治、亀甲龍彦と担当)

  斎藤博幸; 田中秀治

  廣川書店, Apr. 2012, Not refereed

  Find Kyoto University's collection of books :

  
• 「入門 構造生物学」 (pp.33-34 中性子でDNAを見るを担当)

  高エネルギー加速器研究機構構造生物学研究センター編

  共立出版, 24 Apr. 2010, Not refereed

  
• 製剤への物理化学 第3章 69-90頁

  Toshiyuki Chatake; Tatsuhiko Kikkou; Osamu Matsumoto

  廣川書店, 2006, Not refereed

  

Industrial Property Rights

• 特開2005-003489, 特願2003-166553, 高分子の結晶品質を評価する方法

  新村 信雄; 新井 栄揮; 茶竹 俊行

  
• 特開2004-026528, 特願2002-181988, 生体高分子の結晶育成装置

  新村 信雄; 大西 裕季; 新井 栄揮; 茶竹 俊行; 前田 満; 栗原 和男

  
• 特開2003-034600, 特願2001-218424, DNAオリゴマー大型単結晶育成法

  新村 信雄; 新井 栄揮; 茶竹 俊行

  

Awards

• 27 Nov. 2004

  日本結晶学会, 日本結晶学会進歩賞
• 27 Aug. 1996

  日本生化学会, 第4 回JB 論文賞(連名)

External funds: Kakenhi

• Neutron D/H contrast crystallography for hydrogen and hydration analysis of macromolecules

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Toshiyuki Chatake

  From 01 Apr. 2016, To 31 Mar. 2019, Project Closed

  中性子;水素;水和;結晶学;生体分子;D/Hコントラスト;D/Hコントラスト法;水和構造

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K05014/
• Neutron Crystallographic study of hydration structures of DNA and its fluctuation

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  Toshiyuki CHATAKE

  From 01 Apr. 2011, To 31 Mar. 2014, Project Closed

  水和構造;揺らぎ;DNA;中性子

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770176/
• X-ray crystallographic analysis of DNA using temperature-control crystallization technique

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  Toshiyuki CHATAKE

  From 01 Apr. 2006, To 31 Mar. 2009, Project Closed

  物理化学;核酸;薬剤;放射線・X線・粒子線;結晶化;薬学;結晶成長;金属と生体分子;水和構造;放射線・X点・粒子線

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18790030/
• 中性子線を用いたタンパク質の動的な水和水の解析

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields

  Kyoto University

  茶竹 俊行

  From 30 Jun. 2022, To 31 Mar. 2025, Granted

  水和;中性子;構造解析;蛋白質;D/Hコントラスト;タンパク質

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K19271/

External funds: others

• 中性子補足療法におけるカルボラン薬剤・生体分子複合体の細胞内挙動と分子生物学的研究

  財団法人医科学応用研究財団調査研究助成金

  From 2009, To 2010

  茶竹俊行

Education

Teaching subject(s)

• From 01 Apr. 2024, To 31 Mar. 2025

  Applied Neutron Engineering

  C082, Fall, Graduate School of Engineering, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Radiation and Humanity

  U132, Spring, Institute for Liberal Arts and Sciences, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Applied Neutron Engineering

  C082, Fall, Graduate School of Engineering, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Applied Neutron Engineering

  C082, Fall, Graduate School of Engineering, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  応用中性子工学

  Fall, 大学院工学研究科
• From Apr. 2011, To Mar. 2012

  応用中性子工学

  Fall, 工学研究科
• From Apr. 2012, To Mar. 2013

  Applied Neutron Engineering

  Fall, 工学研究科
• From Apr. 2013, To Mar. 2014

  Applied Neutron Engineering

  Fall, 工学研究科
• From Apr. 2014, To Mar. 2015

  Applied Neutron Engineering

  Fall, 工学研究科
• From Apr. 2015, To Mar. 2016

  Applied Neutron Engineering

  Fall, 工学研究科
• From Apr. 2016, To Mar. 2017

  Radiation and Man

  Spring, 全学共通科目
• From Apr. 2016, To Mar. 2017

  Applied Neutron Engineering

  Fall, 工学研究科
• From Apr. 2017, To Mar. 2018

  Radiation and Man

  Spring, 全学共通科目
• From Apr. 2017, To Mar. 2018

  Applied Neutron Engineering

  Fall, 工学研究科
• From Apr. 2018, To Mar. 2019

  Radiation and Man

  Spring, 全学共通科目
• From Apr. 2018, To Mar. 2019

  Applied Neutron Engineering

  Fall, 工学研究科
• From Apr. 2019, To Mar. 2020

  Radiation and Man

  Spring, 全学共通科目
• From Apr. 2019, To Mar. 2020

  Applied Neutron Engineering

  Fall, 工学研究科
• From Apr. 2020, To Mar. 2021

  Radiation and Man

  Spring, 全学共通科目
• From Apr. 2020, To Mar. 2021

  Applied Neutron Engineering

  Fall, 工学研究科
• From Apr. 2021, To Mar. 2022

  Applied Neutron Engineering

  Fall, 工学研究科

Administration

Faculty management (title, position)

• From 01 Jun. 2022

  量子ビーム生体高分子統合研究センター センター員
• From 13 Apr. 2009

  組換えDNA実験等委員会委員
• From 01 Apr. 2007

  動物実験委員会委員
• From 01 Apr. 2014, To 31 Mar. 2018

  地域広報委員会委員
• From 01 Apr. 2015, To 31 Mar. 2016

  所員会議委員