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Mii, Yusuke
Institute for Life and Medical Sciences (LiMe) Assistant Professor
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Last Updated :2025/05/01
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Email Address
mii.yusuke.7j
kyoto-u.ac.jp
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Research History
- From Apr. 2024, To PresentInstitute for Life and Medical Sciences, Kyoto University, Assistant Professor
- From Apr. 2012, To Mar. 2024National Institute for Basic Biology, 分子発生学研究部門, 助教
- From Oct. 2019, To Mar. 2023Japan Science and Technology Agency, さきがけ研究者
- From Apr. 2009, To Mar. 2012JSPS, Research Fellow (DC1)
Profile
Profile
細胞間のコミュニケーションに関わる分泌性シグナル蛋白質に興味をもち、アフリカツメガエル胚を材料に、顕微鏡で見ることにこだわった研究をしています。
分泌性シグナルタンパク質の中でも「モルフォゲン」として知られるWntやFGF、TGF-betaなどは細胞外に分泌されたあと、拡散することなどによって「濃度勾配」を作って働くと考えられてきました。学部生の頃にそもそも濃度勾配ができる時には拡散などの物理的な作用が働くはずと考え、その仕組みを知りたいということで研究を始めましたが、以後一貫して分泌性蛋白質の研究をしています。特にWnt蛋白質の分布や拡散を実際に顕微鏡で見ることに注力していますが、一般的な濃度勾配のドグマからは外れたところで面白い現象がいろいろ見えてきました。最近はWntによって細胞の方向性「平面細胞極性」が揃えられる現象に興味をもって調べているところです。
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70634129External link
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list
Last Updated :2025/05/01
Research
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Papers
- Soluble Frizzled-related proteins promote exosome-mediated Wnt re-secretionThi Hong Nguyen Tran; Ritsuko Takada; Elena Krayukhina; Takahiro Maruno; Yusuke Mii; Susumu Uchiyama; Shinji TakadaCommunications Biology, 01 Mar. 2024, Peer-reviewed
- Understanding and manipulating extracellular behaviors of Wnt ligandsYusuke MiiIn Vitro Cellular & Developmental Biology - Animal, 20 Feb. 2024, Peer-reviewed, Invited, Lead author, Last author, Corresponding author
- Dissection of N-deacetylase and N-sulfotransferase activities of NDST1 and their effects on Wnt8 distribution and signaling in Xenopus embryosMinako Suzuki; Shinji Takada; Yusuke MiiDevelopment, Growth & Differentiation, Feb. 2024, Peer-reviewed, Last author, Corresponding author
- The Helicobacter pylori CagA oncoprotein disrupts Wnt/PCP signaling and promotes hyperproliferation of pyloric gland base cells.Atsushi Takahashi-Kanemitsu; Mengxue Lu; Christopher Takaya Knight; Takayoshi Yamamoto; Takuo Hayashi; Yusuke Mii; Takuya Ooki; Ippei Kikuchi; Akira Kikuchi; Nick Barker; Etsuo A Susaki; Masanori Taira; Masanori HatakeyamaScience signaling, 18 Jul. 2023, Peer-reviewed
- Intercellular exchange of Wnt ligands reduces cell population heterogeneity during embryogenesisYudai Hatakeyama; Nen Saito; Yusuke Mii; Ritsuko Takada; Takuma Shinozuka; Tatsuya Takemoto; Honda Naoki; Shinji TakadaNature Communications, 06 Apr. 2023, Peer-reviewed
- Alignment of cell long axis by unidirectional tension acts cooperatively with Wnt signalling to establish PCPSayuki Hirano; Yusuke Mii; Guillaume Charras; Tatsuo MichiueDevelopment, May 2022, Peer-reviewed
- Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesisKei Yamamoto; Haruko Miura; Motohiko Ishida; Yusuke Mii; Noriyuki Kinoshita; Shinji Takada; Naoto Ueno; Satoshi Sawai; Yohei Kondo; Kazuhiro AokiNature Communications, Dec. 2021, Peer-reviewed
- Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing discShinya Matsuda; Jonas V. Schaefer; Yusuke Mii; Yutaro Hori; Dimitri Bieli; Masanori Taira; Andreas Plückthun; Markus AffolterNature Communications, Dec. 2021, Peer-reviewed
- Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7–mediated Vangl ubiquitination and endoplasmic reticulum–associated degradationDi Feng; Jin Wang; Wei Yang; Jingyu Li; Xiaochen Lin; Fangzi Zha; Xiaolu Wang; Luyao Ma; Nga Ting Choi; Yusuke Mii; Shinji Takada; Michael S. Y. Huen; Yusong Guo; Liang Zhang; Bo GaoScience Advances, May 2021, Peer-reviewed
- Quantitative analyses reveal extracellular dynamics of Wnt ligands in Xenopus embryosYusuke Mii; Kenichi Nakazato; Chan-Gi Pack; Takafumi Ikeda; Yasushi Sako; Atsushi Mochizuki; Masanori Taira; Shinji TakadaeLife, 27 Apr. 2021, Peer-reviewed, Lead author, Corresponding author
- Heparan Sulfate Clusters Regulate Distribution and Signaling of Wnt MorphogensYusuke MiiTrends in Glycoscience and Glycotechnology, 25 Nov. 2020, Peer-reviewed, Invited, Lead author, Last author, Corresponding author
- Improvement of Phycocyanobilin Synthesis for Genetically Encoded Phytochrome-Based OptogeneticsYouichi Uda; Haruko Miura; Yuhei Goto; Kei Yamamoto; Yusuke Mii; Yohei Kondo; Shinji Takada; Kazuhiro AokiACS Chemical Biology, 09 Nov. 2020, Peer-reviewed
- Heparan Sulfate Proteoglycan Clustering in Wnt Signaling and DispersalYusuke Mii; Shinji TakadaFrontiers in Cell and Developmental Biology, 14 Jul. 2020, Peer-reviewed, Invited, Lead author, Corresponding author
- Ripply3 is required for the maintenance of epithelial sheets in the morphogenesis of pharyngeal pouchesYoshihiro Tsuchiya; Yusuke Mii; Kazunori Okada; Mikio Furuse; Tadashi Okubo; Shinji TakadaDevelopment Growth and Differentiation, 01 Feb. 2018, Peer-reviewed
- Assembly of protein complexes restricts diffusion of Wnt3a proteins.Ritsuko Takada; Yusuke Mii; Elena Krayukhina; Yuusuke Maruyama; Kazuhiro Mio; Yoshikazu Sasaki; Takao Shinkawa; Chan-Gi Pack; Yasushi Sako; Chikara Sato; Susumu Uchiyama; Shinji TakadaCommunications biology, 2018, Peer-reviewed, Lead author
- Roles of two types of heparan sulfate clusters in Wnt distribution and signaling in XenopusYusuke Mii; Takayoshi Yamamoto; Ritsuko Takada; Shuji Mizumoto; Makoto Matsuyama; Shuhei Yamada; Shinji Takada; Masanori TairaNature Communications, Dec. 2017, Peer-reviewed, Lead author
- Differences in the secretion and transport of Wnt proteinsShinji Takada; Sayumi Fujimori; Takuma Shinozuka; Ritsuko Takada; Yusuke MiiJournal of Biochemistry, Jan. 2017, Peer-reviewed
- Secreted Wnt "inhibitors" are not just inhibitors: Regulation of extracellular Wnt by secreted Frizzled-related proteinsYusuke Mii; Masanori TairaDEVELOPMENT GROWTH & DIFFERENTIATION, Oct. 2011, Peer-reviewed, Lead author
- Secreted Frizzled-related proteins enhance the diffusion of Wnt ligands and expand their signalling rangeYusuke Mii; Masanori TairaDevelopment, Dec. 2009, Peer-reviewed, Lead author
Misc.
- Heparan Sulfate Clusters Regulate Distribution and Signaling of Wnt MorphogensYusuke MiiTrends in Glycoscience and Glycotechnology, 25 Nov. 2020, Peer-reviewed, Invited, Lead author, Last author, Corresponding author
- Adapter protein Ripply3 is required for epithelial morphogenesis in the mouse pharyngeal pouchYoshihiro Tsuchiya; Tadashi Okubo; Yusuke Mii; Shinji TakadaMECHANISMS OF DEVELOPMENT, Jul. 2017
Presentations
- Understanding planar cell polarity with Xenopus and in vitro systemsYusuke MiiRIKEN BDR Seminar, 20 Sep. 2024, Invited
- Symmetry breaking of planar cell polarity by Wnt.Yusuke MiiInternational Workshop on Multi-scale Biological Plasticity (Komaba, Tokyo), 08 Jan. 2024, Invited
- Multiple positive feedback loops are involved in the symmetry breaking of planar cell polarity (PCP) by Wnt.三井 優輔; 鈴木 美奈子; 小山 宏史; 高田 律子; 松山 誠; 藤森 俊彦; 高田 慎治第46回日本分子生物学会年会 （神戸市）, Dec. 2023
- 一本鎖抗体（scFv）の応用で見えてきた 平面細胞極性におけるダイナミックな ヘパラン硫酸プロテオグリカンの挙動鈴木 美奈子; 日笠 弘基; 平良 眞規; 高田 慎治; 三井 優輔日本動物学会 第94回山形大会, Sep. 2023
- Mutual regulation among Wnt11 and core PCP components is involved in self-organization of planar cell polarity.Yusuke Mii; Minako Suzuki; Hiroshi Koyama; Ritsuko Takada; Makoto Matsuyama; Toshihiko Fujimori; Shinji TakadaWnt Signaling, Gordon Research Conference, Aug. 2023
- Self-organization of planar cell polarity by mutual regulation among Wnt11 and core PCP components.Yusuke Mii; Minako Suzuki; Ritsuko Takada; Hiroshi Koyama; Makoto Matsuyama; Toshihiko Fujimori; Shinji Takada日本発生生物学会第56回大会, 23 Jul. 2023
- Mutual regulations between Wnt11 and core PCP components establish planar cell polarity.Yusuke MiiThe 3rd NINS-Princeton Joint Symposium, 23 Mar. 2023, Invited
- Mutual regulations between Wnt11 and core PCP components establish planar cell polarity.Yusuke Mii; Minako Suzuki; Hiroshi Koyama; Ritsuko Takada; Makoto Matsuyama; Toshihiko Fujimori; Shinji TakadaEMBO Workshop Wnt 2022, 16 Nov. 2022
- 平面細胞極性因子群によるWnt蛋白質の空間パターンの制御三井 優輔第93回 日本動物学会早稲田大会, 08 Sep. 2022, Invited
- Phosphorylation of Vangl2 is involved in local scaffold formation for Wnt11 in the establishment of planar cell polarity.Yusuke Mii; Minako Suzuki; Hiroshi Koyama; Ritsuko Takada; Makoto Matsuyama; Motosuke Tsutsumi; Tomomi Nemoto; Toshihiko Fujimori; Shinji Takada日本発生生物学会第55回大会, 03 Jun. 2022
- Wnt gradient formation by dynamic exchange of abundant heparan sulfate-bound and rare freely diffusing ligandsYusuke Mii; Kenichi Nakazato; Chan-Gi Pack; Takafumi Ikeda; Yasushi Sako; Atsushi Mochizuki; Masanori Taira; Shinji TakadaEMBO Workshop Long-distance cell-cell signalling in development and disease
- Wnt11とコアPCP因子の相互的制御による平面細胞極性（PCP）の形成三井 優輔第44回日本分子生物学会年会, Dec. 2021, Invited
- Feedback regulation among heparan sulfate proteoglycans, Wnt11, and core PCP components are involved in organization of planar cell polarity.Minako Suzuki; Shinji Takada; Yusuke MiiJSDB 54th Meeting, Jun. 2021
- Dynamic exchange of abundant scaffold-bound and rare freely diffusing ligands forms Wnt gradient.Yusuke Mii; Kenichi Nakazato; Chan-Gi Pack; Takafumi Ikeda; Yasushi Sako; Atsushi Mochizuki; Masanori Taira; Shinji TakadaJSDB 54th Meeting, Jun. 2021
- 119th International Titisee ConferenceYusuke Mii119th International Titisee Conference, Mar. 2019, Invited
- Local and mutual regulations between Wnt and planar cell polarity propagate global coordination of cell polarity throughout the tissue.Yusuke MiiJoint Meeting of JSCB 70th & JSDB 51st, 08 Jun. 2018, Invited
- Extracellular behaviors of Wnt ligands in Xenopus embryoYusuke MiiAmphibian development, regeneration, evolution and beyond（広島大学 国際シンポジウム）, 13 Mar. 2018, Invited
- 分泌性シグナル蛋白質Wnt8の分布とシグナル受容を制御するHSのクラスター構造Yusuke Mii27th Proteoglycan Forum, 18 Feb. 2018, Invited
- ツメガエル初期胚での分泌性シグナル蛋白質Wntの挙動を理解する三井 優輔第9回イメージング若手の会 光塾 （於 山口大学）, 05 Sep. 2017, Invited
Awards
- 17 Jul. 2022自然科学研究機構 若手研究者賞
- 22 Mar. 2012東京大学大学院理学系研究科 研究奨励賞(博士)
- 30 May 2014Best Poster Award， 47th Annual Meeting for the JSDB
- 31 Dec. 2021基礎生物学研究所 若手研究者賞(第1位)
External funds: Kakenhi
- Planar cell polarity as self-organization involving localized Wnt proteinGrant-in-Aid for Scientific Research (B)Basic Section 44020:Developmental biology-relatedKyoto University;National Institute for Basic Biology三井 優輔From 01 Apr. 2022, To 31 Mar. 2027, Granted平面細胞極性;Xenopus;Wnt;ヘパラン硫酸プロテオグリカン;PCP;HSPG
- Dynamic coupling of ECM and secreted signaling proteinsGrant-in-Aid for Transformative Research Areas (A)Transformative Research Areas, Section (III)Kyoto University;National Institute for Basic Biology三井 優輔From 01 Apr. 2023, To 31 Mar. 2028, Granted平面細胞極性;ヘパラン硫酸プロテオグリカン;Wnt;PCP;HSPG;Xenopus;ゼブラフィッシュ
- 領域研究「細胞外情報を統御するマルチモーダルECM」の統括と運営Grant-in-Aid for Transformative Research Areas (A)Transformative Research Areas, Section (III)Institute of Physical and Chemical Research藤原 裕展From 01 Apr. 2023, To 31 Mar. 2028, Granted細胞外マトリックス;技術支援;研究資源共有化;若手支援;広報
- An in vitro system of coordinated planar cell polarity.Grant-in-Aid for Challenging Research (Exploratory)Medium-sized Section 44:Biology at cellular to organismal levels, and related fieldsNational Institute for Basic Biology三井 優輔From 30 Jun. 2022, To 31 Mar. 2025, Granted平面細胞極性;再構成系;ライブイメージング
- Planar cell polarity as self-organization involving localized Wnt proteinGrant-in-Aid for Scientific Research (B)Basic Section 44020:Developmental biology-relatedNational Institute for Basic Biology三井 優輔From 01 Apr. 2022, To 31 Mar. 2027, Granted平面細胞極性;Xenopus;Wnt;ヘパラン硫酸プロテオグリカン;HSPG
- Establishment of cell polarity by interactions between Wnt and PCP factors.Grant-in-Aid for Early-Career ScientistsBasic Section 44020:Developmental biology-relatedNational Institute for Basic BiologyYusuke MiiFrom 01 Apr. 2018, To 31 Mar. 2022, Project ClosedXenopus;アフリカツメガエル;Wnt;モルフォゲン;平面細胞極性;PCP;イメージング;超解像イメージング;ヘパラン硫酸
- Localized Wnt protein and morphogenesis.Grant-in-Aid for Challenging Exploratory ResearchNational Institute for Basic BiologyYusuke MiiFrom 01 Apr. 2015, To 31 Mar. 2018, Project ClosedWnt;平面細胞極性;Xenopus;ヘパラン硫酸;PCP;アフリカツメガエル;noncanonical Wnt;細胞骨格
- Dynamics and signal reception of the Wnt morphogen.Grant-in-Aid for Research Activity Start-upNational Institute for Basic BiologyYusuke MIIFrom 31 Aug. 2012, To 31 Mar. 2014, Project ClosedWnt;ヘパラン硫酸;モルフォゲン;sFRP;アフリカツメガエル;拡散
- Evolutionary mechanisms of the gastrula organizer and blastopore formation by focusing on the gene regulatory networkGrant-in-Aid for Challenging Exploratory ResearchThe University of TokyoMasanori TAIRAFrom 01 Apr. 2012, To 31 Mar. 2013, Project Closed転写因子;分泌性因子
- Moleculara mechanism and regulation of the lymphoid organGrant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)Biological SciencesOkazaki Research Facilities, National Institutes of Natural SciencesShinji TAKADAFrom 28 Jun. 2012, To 31 Mar. 2017, Project Closed遺伝子;細胞分化;シグナル伝達;咽頭弓;胸腺;細胞;発生;細胞・組織;発生・分化;免疫学;免疫
