Basic Information

Faculty

• Faculty of Science

Email Address

• Email Address

  nagahara.shiori.3nkyoto-u.ac.jp

Academic Degree

• 25 Mar. 2013

  名古屋大学修士（理学）
• 25 Mar. 2016

  名古屋大学博士（理学）

Academic Resume (Undergraduate School/Majors)

• 名古屋大学, 理学部生命理学科, 卒業

Research History

• From Nov. 2023, To Present

  Kyoto University, Graduate School of Science Division of Biological Sciences, 助教
• From Apr. 2022, To Oct. 2023

  Nagoya University, Institute of Transformative Bio-Molecules, 研究員
• From Apr. 2019, To Mar. 2022

  Nagoya University, Institute of Transformative Bio-Molecules / Graduate School of Science, 研究員
• From Apr. 2016, To Mar. 2019

  名古屋大学トランスフォーマティブ生命分子研究所, 技術補佐員
• From Apr. 2013, To Mar. 2016

  日本学術振興会特別研究員 (DC1)

ID,URL

• J-Global ID

  202201006243410516

researchmap URL

• https://researchmap.jp/sn-20220524

Research

Research Interests

• 原形質連絡
• 細胞間コミュニケーション
• ライブイメージング
• クロマチン動態
• 重複受精
• 植物生殖

Research Areas

• Life sciences, Plants: molecular biology and physiology

Papers

• The peri-germ cell membrane: poorly characterized but key interface for plant reproduction

  Naoya Sugi; Andrea R. M. Calhau; Nathanaël M. A. Jacquier; Marina Millan-Blanquez; Jörg D. Becker; Kevin Begcy; Frédéric Berger; Cécile Bousquet-Antonelli; Daniel Bouyer; Giampiero Cai; Alice Y. Cheung; Sílvia Coimbra; Philipp Denninger; Thomas Dresselhaus; José A. Feijó; John E. Fowler; Danny Geelen; Ueli Grossniklaus; Tetsuya Higashiyama; David Honys; Tomoko Igawa; Gwyneth Ingram; Yvon Jaillais; Mark A. Johnson; Mariko Kato; Miki Kawachi; Tomokazu Kawashima; Yu-Jin Kim; Hong-Ju Li; Sébastien Mongrand; Kazuki Motomura; Shiori Nagahara; Kohdai P. Nakajima; Brad Nelms; Li-Jia Qu; Arp Schnittger; Stefan Scholten; Stefanie Sprunck; Meng-Xiang Sun; David Twell; Dolf Weijers; Wei-Cai Yang; Daisuke Maruyama; Thomas Widiez

  Nature Plants, 15 Oct. 2024

  
• Development of a Versatile System to Facilitate Targeted Knockout/Elimination Using CRISPR/Cas9 for Highly Duplicated Gene Families inArabidopsisSexual Reproduction

  Hidenori Takeuchi; Shiori Nagahara

  bioRxiv, 26 Apr. 2024

  
• The Chaperone NASP Contributes to de Novo Deposition of the Centromeric Histone Variant CENH3 in Arabidopsis Early Embryogenesis

  Hidenori Takeuchi; Shiori Nagahara; Tetsuya Higashiyama; Frédéric Berger

  Plant And Cell Physiology, 10 Apr. 2024, Peer-reviewed

  
• Deep imaging reveals dynamics and signaling in one-to-one pollen tube guidance

  Yoko Mizuta; Daigo Sakakibara; Shiori Nagahara; Ikuma Kaneshiro; Takuya T Nagae; Daisuke Kurihara; Tetsuya Higashiyama

  EMBO Reports, 21 May 2024, Peer-reviewed

  
• Deep Imaging and Optical Manipulation of Double Fertilization

  Shiori Nagahara; Tetsuya Higashiyama

  CYTOLOGIA, 25 Sep. 2022, Lead author, Corresponding author

  
• Optical Clearing of Plant Tissues for Fluorescence Imaging

  Daisuke Kurihara; Yoko Mizuta; Shiori Nagahara; Yoshikatsu Sato; Tetsuya Higashiyama

  Journal of Visualized Experiments, 05 Jan. 2022, Peer-reviewed

  
• ClearSeeAlpha: Advanced Optical Clearing for Whole-Plant Imaging

  Daisuke Kurihara; Yoko Mizuta; Shiori Nagahara; Tetsuya Higashiyama

  Plant and Cell Physiology, 10 Nov. 2021, Peer-reviewed

  
• Detection of a biolistic delivery of fluorescent markers and CRISPR/Cas9 to the pollen tube

  Shiori Nagahara; Tetsuya Higashiyama; Yoko Mizuta

  Plant Reproduction, Sep. 2021, Peer-reviewed, Lead author

  
• Polyspermy Block in the Central Cell During Double Fertilization of Arabidopsis thaliana

  Shiori Nagahara; Hidenori Takeuchi; Tetsuya Higashiyama

  Frontiers in Plant Science, 12 Jan. 2021, Peer-reviewed, Lead author, Corresponding author

  
• The male gamete membrane protein DMP9/DAU2 is required for double fertilization in flowering plants

  Taro Takahashi; Toshiyuki Mori; Kenji Ueda; Lixy Yamada; Shiori Nagahara; Tetsuya Higashiyama; Hitoshi Sawada; Tomoko Igawa

  Development, 01 Dec. 2018, Peer-reviewed

  
• Pollen differentiation as well as pollen tube guidance and discharge are independent of the presence of gametes

  Barbara Glöckle; Wojciech J. Urban; Shiori Nagahara; Ellen D. Andersen; Tetsuya Higashiyama; Paul E. Grini; Arp Schnittger

  Development, 01 Jan. 2017, Peer-reviewed

  
• Pollen tube contents initiate ovule enlargement and enhance seed coat development without fertilization

  Ryushiro D. Kasahara; Michitaka Notaguchi; Shiori Nagahara; Takamasa Suzuki; Daichi Susaki; Yujiro Honma; Daisuke Maruyama; Tetsuya Higashiyama

  Science Advances, 07 Oct. 2016, Peer-reviewed

  
• Generation of a homozygous fertilization-defective gcs1 mutant by heat-inducible removal of a rescue gene

  Shiori Nagahara; Hidenori Takeuchi; Tetsuya Higashiyama

  Plant Reproduction, Mar. 2015, Peer-reviewed, Lead author

  
• Double fertilization on the move

  Yuki Hamamura; Shiori Nagahara; Tetsuya Higashiyama

  Current Opinion in Plant Biology, Feb. 2012, Peer-reviewed

  

Presentations

• 植物細胞間コミュニケーションを担う原形質連絡のイメージング解析

  永原史織; 中村匡良; 植村知博; 大山泰生; 鈴木友美; 望月伸悦; 黒谷賢一; 野田口理孝

  日本植物学会第88回大会, 14 Sep. 2024

  
• 助細胞における花粉管誘引シグナルの極性分泌ダイナミクスの解析

  永原史織; 丸山大輔; 東山哲也; 水多陽子; 武内秀憲

  日本植物学会第87回大会, 07 Sep. 2023

  
• Maternal epigenetic states contribute to centromeric CENH3 deposition and early embryogenesis in Arabidopsis

  Shiori Nagahara; Tetsuya Higashiyama; Frédéric Berger; Hidenori Takeuchi

  Cold Spring Harbor Asia, Integrative Epigenetics in Plants

  
• シロイヌナズナ受精卵の発生過程におけるCENH3マークの新規形成を制御するクロマチンクロストークの解析

  永原史織; 東山哲也; Frédéric Berger; 武内秀憲

  第63回日本植物生理学会年会

  
• 花粉への一過的遺伝子導入系による花粉管および精細胞の動態解析

  永原史織; 栗原大輔; 東山哲也; 水多陽子

  第60回日本植物生理学会年会

  
• パーティクルボンバードメント法による植物生殖細胞へのCRISPR/Cas9の導入

  永原史織; 栗原大輔; 東山哲也; 水多陽子

  生命科学系学会合同年次大会（第40回 日本分子生物学会年会）

  
• ライブイメージングによる植物生殖過程の細胞動態の解明

  永原史織; 栗原大輔; 水多陽子; 東山哲也

  日本顕微鏡学会第73回学術講演会, Invited

  
• Live-cell analysis of sperm cell positioning during double fertilization in Arabidopsis thaliana

  Shiori Nagahara; Yuki Hamamura; Hidenori Takeuchi; Tetsuya Higashiyama

  24th International Congress on Sexual Plant Reproduction

  
• 受精異常突然変異体 gcs1のホモ接合変異体の作出と生理学的解析

  永原史織; 武内秀憲; 東山哲也

  第56回日本植物生理学会年会

  
• Live-cell analysis of gamete communication during double fertilization in Arabidopsis thaliana by laser disruption technique

  Shiori Nagahara; Yuki Hamamura; Hidenori Takeuchi; Tetsuya Higashiyama

  23rd International Congress on Sexual Plant Reproduction

  
• 顕微細胞操作による重複受精時の精細胞分配機構の解析

  永原史織; 須崎大地; 武内秀憲; 浜村有希; 東山哲也

  日本植物学会第77回大会

  
• 光細胞操作を用いたシロイヌナズナ重複受精における雌雄配偶子間コミュニケーションの解析

  永原史織; 須崎大地; 武内秀憲; 浜村有希; 東山哲也

  第54回日本植物生理学会年会

  
• Analyses of double fertilization mechanism in Arabidopsis thaliana by optical manipulation techniques

  Shiori Nagahara; Daichi Susaki; Hidenori Takeuchi; Yuki Hamamura; Tetsuya Higashiyama

  10th International Congress on Plant Molecular Biology

  
• レーザー細胞操作によるシロイヌナズナ重複受精機構の解析

  永原史織; 須崎大地; 武内秀憲; 浜村有希; 東山哲也

  日本植物学会第76回大会

  
• 光細胞操作によるシロイヌナズナ雌雄生殖細胞機能の解析

  永原史織; 須崎大地; 武内秀憲; 浜村有希; 東山哲也

  日本植物形態学会第24回大会, 14 Sep. 2012

  

Industrial Property Rights

• 特願2020-169978, 花粉への物質導入方法

  栗原陽子; 皆川吉; 村田翔太朗; 永原史織

  
• 特願2018-079316, 植物ゲノム編集方法

  水多陽子; 永原史織

  

Education

Teaching subject(s)

• From 01 Apr. 2024, To 31 Mar. 2025

  Special Study CourseII(Biological Sciences) 9

  8712, Year-long, Faculty of Science, 12
• From 01 Apr. 2024, To 31 Mar. 2025

  Laboratory work in Biological Science C

  3764, Spring, Faculty of Science, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminars in Biological Science B

  4732, Fall, Faculty of Science, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar on Plant Physiology A

  8516, Spring, Graduate School of Science, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Lectures : Plant Physiology B

  8501, Fall, Graduate School of Science, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Lectures : Plant Physiology A

  8500, Spring, Graduate School of Science, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar on Plant Physiology D

  8519, Fall, Graduate School of Science, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar on Plant Physiology C

  8518, Spring, Graduate School of Science, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar on Plant Physiology B

  8517, Fall, Graduate School of Science, 2