Basic Information

Professional Memberships

• From Dec. 2020, To Present

  Japan Society for Bioscience, Biotechnology, and Agrochemistry
• From 2019, To Present

  SPring-8ユーザー共同体(SPRUC)X線自由電子レーザーXFEL利用研究会会員
• From Aug. 2012, To Present

  SPring-8ユーザー共同体(SPRUC)放射光構造生物学研究会
• From Apr. 2002, To Present

  The Japanese Biochemical Society

Academic Degree

• M.S. in Agriculture(Characterization of stilbene synthase genes in Japanese red pine (Pinus densiflora).)
• Ph.D.(Structural and functional diversity of stilbene synthases from Japanese red pine (Pinus densiflora).)

Academic Resume (Graduate Schools)

• Kyoto University, Graduate School of Agriculture, Division of Applied Life Sciences, 修了
• Kyoto University, Graduate School of Agriculture, Division of Applied Life Sciences, 修了

ID,URL

• J-Global ID

  201401042230227449

researchmap URL

• https://researchmap.jp/2418

Research

Research Topics, Overview of the research

• Research Topics

  Molecular mechanism of ABC proteins

• Overview of the research

  ABC (ATP-Binding Cassette) proteins are transmembrane proteins with well-conserved ATP binding domains. In humans, 48 ​​ABC proteins play an important role in maintaining our health. ABC proteins mainly transport lipophilic compounds and are responsible for maintaining our body's defense against xenobiotics as well as maintaining lipid homeostasis. Recently, by studying the three-dimensional structures and function of an MDR1(P-glycoprotein), the most representative ABC protein, we revealed the structural change caused by the binding of ATP to the ATP-binding domain is propagated to the transmembrane domain and the whole molecule by bringing about dynamic structural transformation. We also clarified the ingenious mechanism of the MDR1 molecule that squeezes lipophilic compounds out of cells. Based on the research infrastructure established in MDR1 so far, we are trying to clarify the molecular mechanism of other important human ABC proteins (for example, lipid-transporting ABC proteins) that are still unknown. This study has the potential to provide a blueprint for the development of new therapeutic strategies targeting ABC proteins.

Research Interests

• 脂質代謝
• nascent HDL
• drug discovery
• structure based molecular mechanism
• conformational change
• conformation and activity relationship
• biological macromolecule

Research Areas

• Life sciences, Nutrition and health science, nascent HDL
• Life sciences, Molecular biology
• Life sciences, Cell biology
• Life sciences, Pharmacology
• Life sciences, Structural biochemistry
• Life sciences, Applied biochemistry

Papers

• The role of ATP hydrolysis in conformational changes of human P-glycoprotein in living cells

  Ryota Futamata; Fumihiko Ogasawara; Takafumi Ichikawa; Atsushi Kodan; Yasuhisa Kimura; Noriyuki Kioka; Kazumitsu Ueda

  bioRxiv, Nov. 2019

  
• Structure and Mechanism of a Eukaryotic ABC Multidrug Transporter

  KODAN Atsushi; YAMAGUCHI Tomohiro; NAKATSU Toru; KATO Hiroaki

  Nihon Kessho Gakkaishi, 2014

  
• ABCB1/MDR1/P-gp employs an ATP-dependent twist-and-squeeze mechanism to export hydrophobic drugs.

  Atsushi Kodan; Ryota Futamata; Yasuhisa Kimura; Noriyuki Kioka; Toru Nakatsu; Hiroaki Kato; Kazumitsu Ueda

  FEBS letters, Mar. 2021, Peer-reviewed

  
• ABC proteins in evolution.

  Fumihiko Ogasawara; Atsushi Kodan; Kazumitsu Ueda

  FEBS letters, Dec. 2020, Peer-reviewed

  
• In vivo FRET analyses reveal a role of ATP hydrolysis–associated conformational changes in human P-glycoprotein

  Ryota Futamata; Fumihiko Ogasawara; Takafumi Ichikawa; Atsushi Kodan; Yasuhisa Kimura; Noriyuki Kioka; Kazumitsu Ueda

  Journal of Biological Chemistry, 10 Apr. 2020, Peer-reviewed

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• Inward- and outward-facing X-ray crystal structures of homodimeric P-glycoprotein CmABCB1.

  Kodan A; Yamaguchi T; Nakatsu T; Matsuoka K; Kimura Y; Ueda K; Kato H

  Nature communications, Jan. 2019, Peer-reviewed

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• Experimental phase determination with selenomethionine or mercury-derivatization in serial femtosecond crystallography

  Keitaro Yamashita; Naoyuki Kuwabara; Takanori Nakane; Tomohiro Murai; Eiichi Mizohata; Michihiro Sugahara; Dongqing Pan; Tetsuya Masuda; Mamoru Suzuki; Tomomi Sato; Atsushi Kodan; Tomohiro Yamaguchi; Eriko Nango; Tomoyuki Tanaka; Kensuke Tono; Yasumasa Joti; Takashi Kameshima; Takaki Hatsui; Makina Yabashi; Hiroshi Manya; Tamao Endo; Ryuichi Kato; Toshiya Senda; Hiroaki Kato; So Iwata; Hideo Ago; Masaki Yamamoto; Fumiaki Yumoto; Toru Nakatsu

  IUCRJ, Sep. 2017, Peer-reviewed

  
• An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography

  Keitaro Yamashita; Dongqing Pan; Tomohiko Okuda; Michihiro Sugahara; Atsushi Kodan; Tomohiro Yamaguchi; Tomohiro Murai; Keiko Gomi; Naoki Kajiyama; Eiichi Mizohata; Mamoru Suzuki; Eriko Nango; Kensuke Tono; Yasumasa Joti; Takashi Kameshima; Jaehyun Park; Changyong Song; Takaki Hatsui; Makina Yabashi; So Iwata; Hiroaki Kato; Hideo Ago; Masaki Yamamoto; Toru Nakatsu

  SCIENTIFIC REPORTS, Sep. 2015, Peer-reviewed

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• Luciferin-regenerating enzyme solved by SIRAS using XFEL (refined against Hg derivative data)

  Yamashita K; Pan D; Okuda T; Sugahara M; Kodan A; Yamaguchi T; Murai T; Gomi K; Kajiyama N; Mizohata E; Suzuki M; Nango E; Tono K; Joti Y; Kameshima T; Park J; Song C; Hatsui T; Yabashi M; Iwata S; Kato H; Ago H; Yamamoto M; Nakatsu T

  Worldwide Protein Data Bank, 2015, Peer-reviewed

  
• Luciferin-regenerating enzyme solved by SAD using synchrotron radiation at room temperature

  Yamashita K; Pan D; Okuda T; Sugahara M; Kodan A; Yamaguchi T; Murai T; Gomi K; Kajiyama N; Mizohata E; Suzuki M; Nango E; Tono K; Joti Y; Kameshima T; Park J; Song C; Hatsui T; Yabashi M; Iwata S; Kato H; Ago H; Yamamoto M; Nakatsu T

  Worldwide Protein Data Bank, 2015, Peer-reviewed

  
• Structural basis for gating mechanisms of a eukaryotic P-glycoprotein homolog

  Atsushi Kodan; Tomohiro Yamaguchi; Toru Nakatsu; Keita Sakiyama; Christopher J. Hipolito; Akane Fujioka; Ryo Hirokane; Keiji Ikeguchi; Bunta Watanabe; Jun Hiratake; Yasuhisa Kimura; Hiroaki Suga; Kazumitsu Ueda; Hiroaki Kato

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Mar. 2014, Peer-reviewed

  
• Deleting two C-terminal alpha-helices is effective to crystallize the bacterial ABC transporter Escherichia coli MsbA complexed with AMP-PNP

  Kanako Terakado; Atsushi Kodan; Hiroaki Nakano; Yasuhisa Kimura; Kazumitsu Ueda; Toru Nakatsu; Hiroaki Kato

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Mar. 2010, Peer-reviewed

  
• Functional role of the linker region in purified human P-glycoprotein

  Tomomi Sato; Atsushi Kodan; Yasuhisa Kimura; Kazumitsu Ueda; Toru Nakatsu; Hiroaki Kato

  FEBS JOURNAL, Jul. 2009, Peer-reviewed

  
• Improved expression and purification of human multidrug resistance protein MDR1 from baculovirus-infected insect cells

  Atsushi Kodan; Hiroyuki Shibata; Takashi Matsumoto; Kanako Terakado; Keita Sakiyama; Michinori Matsuo; Kazumitsu Ueda; Hiroaki Kato

  PROTEIN EXPRESSION AND PURIFICATION, Jul. 2009, Peer-reviewed

  
• Selective labeling of tag-fused protein by tryptophan-sensitized luminescence of a terbium complex

  Tasuku Hirayama; Masayasu Taki; Atsushi Kodan; Hiroaki Kato; Yukio Yamamoto

  CHEMICAL COMMUNICATIONS, 2009, Peer-reviewed

  
• Cholesterol fill-in model: mechanism for substrate recognition by ABC proteins

  Yasuhisa Kimura; Atsushi Kodan; Michinori Matsuo; Kazumitsu Ueda

  JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, Dec. 2007, Peer-reviewed

  
• A stilbene synthase from Japanese red pine (Pinus densiflora): Implications for phytoalexin accumulation and down-regulation of flavonoid biosynthesis

  A Kodan; H Kuroda; F Sakai

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Mar. 2002, Peer-reviewed

  
• Simultaneous expression of stilbene synthase genes in Japanese red pine (Pinus densiflora) seedlings

  A Kodan; H Kuroda; F Sakai

  JOURNAL OF WOOD SCIENCE, 2001, Peer-reviewed

  
• An O-methyltransferase (OMT) cDNA clone in Japanese red pine (Pinus densiflora)

  Kazumi Etoh; Atsushi Kodan; Fukumi Sakai; Hiroyuki Kuroda

  Wood Research, 2000, Peer-reviewed

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• Characterization of stilbene synthase genes in Japanese red pine (Pinus densiflora)

  Atsushi Kodan; Hiroyuki Kuroda; Fukumi Sakai

  Wood Research, 1999, Peer-reviewed, Lead author

  
• Molecular Cloning and Expression of the Canine Metallothionein-III Gene

  S. Kojima; A. Shimada; A. Kodan; K. Kobayashi; T. Morita; Y. Yamano; T. Umemura

  Canadian Journal of Veterinary Research, 1998, Peer-reviewed

  

Misc.

• Direct Visualization of ABCA1 Reconstituted in Nanodisc by High-Speed Atomic Force Microscopy (HS-AFM)

  Atsushi Kodan; Romain Amyo; Yasuhisa Kimura; Noriyuki Kodera; Kazumitsu Ueda

  ABC2023, the 9th FEBS Special Meeting “ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Disease”, 26 Feb. 2023, Lead author

  
• Expression and purification of a lipid efflux ABC protein aiming for Cryo-EM analysis

  小段篤史; 光岡薫; 木村泰久; 木岡紀幸; 植田和光

  日本農芸化学会大会講演要旨集(Web), 2021, Lead author

  
• 多剤排出ポンプMDR1の薬剤絞り出しメカニズム

  小段篤史; 木村泰久; 植田和光

  トランスポーター研究会年会抄録集, 2020, Lead author

  
• 多剤排出ポンプMDR1の薬剤絞り出し機構

  小段篤史; 木村泰久; 植田和光

  ファルマシア(Web), 2020, Lead author

  
• X 線自由電子レーザーを用いたP糖タンパク質ホモログの連続フェムト秒X線結晶構造解析

  大山諒; 潘東青; 中津亨; 佐藤友美; 山口知宏; 小段篤史; 植田和光; 岩田想; 加藤博章

  第14回次世代を担う若手のためのフィジカル・ファーマフォーラム, 28 Aug. 2016

  
• 高分解能結晶構造に基づいたABCトランスポーターの分子機構

  小段篤史

  「多様性が切り拓く膜輸送研究の新時代」第11回トランスポーター研究会年会, 03 Jul. 2016, Invited, Lead author

  
• Serial Femtosecond Crystallography of ABC Transporter

  Oyama, R; Pan, D; Nakatsu, T; Sato, T; Yamaguchi, T; Kodan, A; Ueda, K; Iwata. S; Kato, H

  6th Special Meeting, ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases (ABC2016), Mar. 2016

  
• Crystallographic study of the ABC transporter CmABCB1 covalently bound to substrate derivatives

  Matsuoka, K; Hirokane, R; Kodan, A; Yamaguchi, T; Nakatsu, T; Ueda, K; Kato, H

  6th Special Meeting, ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases (ABC2016), Mar. 2016

  
• X線自由電子レーザーを用いた新規タンパク質の立体構造決定

  山下恵太郎; 潘東青; 奥田智彦; 菅原道泰; 小段篤史; 山口知宏; 村井智洋; 溝端栄一; 鈴木守; 南後恵理子; 登野健介; 城地保昌; 亀島敬; 初井宇記; 矢橋牧名; 岩田想; 岩田想; 加藤博章; 加藤博章; 吾郷日出夫; 山本雅貴; 中津亨; 中津亨

  日本薬学会年会要旨集(CD-ROM), Mar. 2016

  
• 真核生物由来ABC輸送体の分子機構

  小段篤史; 植田和光; 加藤博章

  日本生化学会大会(Web), 2016, Lead author

  
• シリアル結晶学における重原子誘導体を用いた位相決定

  山下恵太郎; 潘東青; 長谷川和也; 菅原道泰; 村井智洋; 小段篤史; 溝端栄一; 鈴木守; 鈴木守; 桝田哲哉; 桝田哲哉; 平田邦生; 加藤博章; 加藤博章; 吾郷日出夫; 熊坂崇; 山本雅貴; 中津亨; 中津亨

  日本結晶学会年会講演要旨集, Oct. 2015

  
• X 線自由電子レーザーを用いたタンパク質 X 線結晶 構造解析

  潘東青; 中津亨; 山口知宏; 小段篤史; 小川和浩; 松嶌一樹; 山下恵太郎; 城地保昌; 登野健介; 矢橋牧名; 鈴木守; 南後恵理子; 岩田想; 加藤博章

  日本薬学会第135年会, 27 Mar. 2015

  
• 測定温度の違いがもたらすタンパク質X線結晶構造への影響

  村井智洋; 潘東青; 小段篤史; 山下恵太郎; 上野剛; 加藤博章

  日本薬学会近畿支部総会, 25 Mar. 2015

  
• SACLAによるABCトランスポーターおよびホタル・ルシフェラーゼのX線結晶構造解析(第2報)

  潘東青; 中津亨; 山口知宏; 小段篤史; 小川和浩; 松嶌一樹; 山下恵太郎; 城地保昌; 登野健介; 矢橋牧名; 鈴木守; 南後恵理子; 岩田想; 加藤博章

  平成26年度日本結晶学会年会, 01 Nov. 2014

  
• ABC多剤排出トランスポーターの立体構造の特徴

  山口知宏; 小段篤史; 中津亨; 植田和光; 加藤博章

  第87回日本生化学会大会, 15 Oct. 2014, Invited

  
• SACLAによるABCトランスポーターおよびホタル・ルシフェラーゼのX線結晶構造解析

  潘東青、中津亨、山口知宏、小段篤史、小川和浩、松嶌一樹、城地保昌、登野健介、矢橋牧名、鈴木守、南後恵理子、岩田想、加藤博章

  第１４回日本蛋白質科学会年会, 25 Jun. 2014

  
• SACLAおよびSPring-8において得られるタンパク質結晶構造の比較

  村井智洋; 潘東青; 山下恵太郎; 菅原道泰; 小段篤史; 山口知宏; 上野 剛; 南後恵理子; 登野健介; 城地保昌; 亀島敬; Changyong Song; 初井宇記; 矢橋牧名; 岩田想; 吾郷日出夫; 山本雅貴; 加藤博章; 中津 亨

  第15回日本蛋白質科学会年会, 25 Jun. 2014

  
• Crystal structure of an ABC multidrug exporter from Cyanidioschyzon merolae, a thermophilic unicellular eukaryote

  Atsushi Kodan; Tomohiro Yamaguchi; Toru Nakatsu; Keita Sakiyama; Christopher J. Hipolito; Akane Fujioka; Ryo Hirokane; Yasuhisa Kimura; Hiroaki Suga; Kazumitsu Ueda; Hiroaki Kato

  ABC2014, 5th FEBS Special Meeting, ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases, Mar. 2014, Invited, Lead author

  
• Structure-based functional aspects of the ABC multidrug transporter, CmABCB1 from a thermophilic eukaryote

  Tomohiro Yamaguchi; Atsushi Kodan; Toru Nakatsu; Keita Sakiyama; Christopher J. Hipolito; Akane Fujioka; Ryo Hirokane; Yasuhisa Kimura; Hiroaki Suga; Kazumitsu Ueda; Hiroaki Kato

  ABC2014, 5th FEBS Special Meeting, ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases, Mar. 2014

  
• ABC多剤排出トランスポーターにおける基質取込の立体構造基盤

  山口知宏; 小段篤史; 中津亨; 植田和光; 加藤博章

  日本薬学会年会要旨集(CD-ROM),134th,ROMBUNNO.29PML-047, 2014

  
• 好熱性真核生物のABC多剤排出トランスポーターのX線結晶構造解析

  廣兼諒; 小段篤史; 中津亨; 山口知宏; 植田和光; 加藤博章

  日本蛋白質科学会年会プログラム・要旨集,13th,83, 2013

  
• 創薬ターゲット蛋白質の迅速構造解析法の開発~SACLAにおけるシリアルフェムト秒X線結晶構造解析~

  南後恵理子; 鈴木守; 田中里枝; 登野健介; 城地保昌; 亀島敬; PARK Jaehyun; SONG Changyong; 初井宇記; 矢橋牧名; 潘東青; 小段篤史; 山口知宏; 中津亨; 加藤博章; 田中良樹; 寿野千代; 伊原健太郎; 豊田洋輔; 島村達郎; 溝端栄一; 北郷悠; 高木淳一; 石谷隆一郎; 濡木理; GALLAT Francois‐Xavier; 山田悠介; 松垣直宏; 湯本史明; CHAVAS Leonar; M. G; 岩田想

  日本結晶学会年会講演要旨集,2013,92, 2013

  
• トランスポーターにおけるATP駆動力共役メカニズムの立体構造基盤

  山口知宏; 小段篤史; 中津亨; 藤岡あかね; 植田和光; 加藤博章

  公開シンポジウム「ゆらぎと水―生命のエネルギーと機能の分子機構を探る」, Sep. 2012

  
• 結晶構造に基づく多剤排出型ABCトランスポーターメカニズムの解明

  藤岡あかね; 山口知宏; 小段篤史; 中津亨; 植田和光; 加藤博章

  次世代を担う若手ファーマ・バイオフォーラム講演要旨集,11th,49, Sep. 2012

  
• X-ray crystallographic analysis of an exporter-type ABC transporter

  Atsushi Kodan; Tomohiro Yamaguchi; Toru Nakatsu; Keita Sakiyama; Akane Fujioka; Kazumitsu Ueda; Hiroaki Kato

  ABC2012, the 4th FEBS Special Meeting “ATP-Binding Cassette Proteins: From Multidrug Resistance to Genetic Disease” Hotel Grauer Bär (Innsbruck, Austria), Mar. 2012, Lead author

  
• 好熱性紅藻由来ABCトランスポーターの輸送基質特異性の検討

  山口知宏; 木村泰久; 小段篤史; 池口圭司; 渡辺文太; 平竹潤; 中津亨; 植田和光; 加藤博章

  生体膜と薬物の相互作用シンポジウム講演要旨集,34th,120-121, 2012

  
• 好熱性真核生物由来の多剤排出ABCトランスポーターの機能解析

  山口知宏; 崎山慶太; 地主遼平; 佐藤友美; 小段篤史; 中津亨; 植田和光; 加藤博章

  日本薬学会年会要旨集,131st,3,116, 2011

  
• 好熱性紅藻由来の基質排出型ABCトランスポーターの基質特異性の解析

  山口知宏; 崎山慶太; 佐藤友美; 小段篤史; 中津亨; 植田和光; 加藤博章

  日本蛋白質科学会年会プログラム・要旨集,11th,134, 2011

  
• Improved expression and purification of human multidrug resistance protein MDR1 from baculovirus-infected insect cells

  A. Kodan; K. Terakado; K. Sakiyama; M. Matsuo; K. Ueda; H. Kato

  ABC2010, the 3rd FEBS Special Meeting “ATP-Binding Cassette Proteins: From Multidrug Resistance to Genetic Disease”, Mar. 2010, Lead author

  
• Deleting two C-terminal α-helices is effective to crystallize the bacterial ABC transporter Escherichia coli MsbA complexed with AMP-PNP

  K. Terakado; A. Kodan; H. Nakano; Y Kimura; K. Ueda; T Nakatsu; H. Kato

  ABC2010, the 3rd FEBS Special Meeting “ATP-Binding Cassette Proteins: From Multidrug Resistance to Genetic Disease”, Mar. 2010

  
• Functional role of the linker region in purified human P-glycoprotein

  T. Sato; A. Kodan; Y. Kimura; K. Ueda; T. Nakatsu; H. Kato

  ABC2010, the 3rd FEBS Special Meeting “ATP-Binding Cassette Proteins: From Multidrug Resistance to Genetic Disease”, Mar. 2010

  
• 好熱性紅藻Cyanidioschyzon merolae由来のABCトランスポーターの発現,精製,及び機能解析

  崎山慶太; 山口知宏; 小段篤史; 中津亨; 佐藤友美; 植田和光; 加藤博章

  日本蛋白質科学会年会プログラム・要旨集,10th,162, 2010

  
• 好熱性紅藻由来ABCトランスポーターの多剤排出活性の特徴

  山口知宏; 崎山慶太; 池口圭司; 地主遼平; 佐藤友美; 渡辺文太; 小段篤史; 中津亨; 平竹潤; 植田和光; 加藤博章

  生体膜と薬物の相互作用シンポジウム講演要旨集,32nd,61-62, 2010

  
• Isolation and biochemical characterization of an ABC transporter from a thermophilic red alga, Cyanidioschyzon merolae

  YAMAGUCHI Tomohiro; SAKIYAMA Keita; KODAN Atsushi; NAKATSU Toru; UEDA Kazumitsu; KATO Hiroaki

  第31回生体膜と薬物の相互作用シンポジウム講演要旨集, Nov. 2009

  
• Improved expression and purification of human multidrug resistance protein MDR1 from baculovirus-infected insect cells

  A. Kodan; K. Terakado; K. Sakiyama; M. Matsuo; K. Ueda; H. Kato

  The 5th iCeMS International Symposium, 28 May 2009, Lead author

  
• Improved expression and purification of human multidrug resistance protein MDR1 from baculovirus-infected insect cells

  A. Kodan; K. Terakado; K. Sakiyama; M. Matsuo; K. Ueda; H. Kato

  The 5th Takeda Science Foundation Symposium on PharmaSciences “Bioactive Lipid Molecules and Transporters”, 25 May 2009, Lead author

  
• 多剤排出トランスポーターP糖タンパク質のリンカー領域の薬物輸送における役割

  佐藤友美; 小段篤史; 木村泰久; 植田和光; 中津亨; 加藤博章

  日本薬学会年会要旨集,129th,4,256, 2009

  
• メタノール資化性酵母Pichia pastorisを用いたABCタンパク質の大量生産

  小段篤史; 崎山慶太; 山口知宏; 中津亨; 植田和光; 加藤博章

  生化学,ROMBUNNO.2P-332, 2009, Lead author

  
• C末端の二本のαヘリックスの欠失による大腸菌由来ABCトランスポーターMsbA結晶の改良

  寺角香菜子; 小段篤史; 中野博明; 木村泰久; 中津亨; 植田和光; 加藤博章

  生化学,ROMBUNNO.2P-316, 2009

  
• BIOFLO310を用いた大腸菌高密度培養による膜タンパク質の効率的生産系

  KODAN ATSUSHI; TERAKADO KANAKO; SUEGAMI SHINJI; KUBO TAKESHI; UEDA KAZUMITSU; NISHIKAWA SHIGEMICHI; KATO HIROAKI

  生化学, 2008, Lead author

  
• 構造解析に適した真核生物由来のABCトランスポーターのスクリーニング

  崎山慶太; 木村泰久; 小段篤史; 中津亨; 植田和光; 加藤博章

  日本薬学会年会要旨集,128th,4,65, 2008

  
• 多剤排出トランスポーターP糖タンパク質におけるリンカー領域の役割

  佐藤友美; 小段篤史; 木村泰久; 中津亨; 加藤博章

  日本蛋白質科学会年会プログラム・要旨集,7th,131, 2007

  
• ABC(ATP-binding cassette)タンパク質の構造解析が拓く創薬の未来

  小段篤史; 木村泰久; 加藤博章; 植田和光

  未来材料, Oct. 2006, Peer-reviewed, Lead author

  
• 昆虫細胞Sf+を用いたヒトP 糖タンパク質の大量精製

  佐藤友美; 小段篤史; 寺角香菜子; 崎山慶太; 木村泰久; 中津 亨; 加藤博章

  第27回生体膜と薬物の相互作用シンポジウム, 28 Nov. 2005

  
• 昆虫細胞Sf+を用いたヒトP糖タンパク質の大量精製

  TERAKADO KANAKO; KODAN ATSUSHI; SATO TOMOMI; SHIBATA HIROYUKI; UEDA KAZUMITSU; KATO HIROAKI

  日本薬学会年会要旨集, 05 Mar. 2005

  
• Large scale purification of P-glycoprotein from Sf9 insect cells

  小段篤史; 柴田洋之; 松本崇; 松尾道憲; 植田和光; 加藤博章

  第76回日本生化学大会, Oct. 2003, Lead author

  
• アカマツのスチルベン合成酵素群の性質 -スチルベノイド・フラボノイドの生合成制御

  小段篤史; 黒田宏之; 酒井富久美

  第52回日本木材学会大会, Apr. 2002, Lead author

  
• アカマツO-メチル基転移酵素cDNA群の性質

  下村文恵; 江藤和美; 小段篤史; 酒井富久美

  第52回日本木材学会大会, Apr. 2002

  
• Stilbene Synthase from Japanese Red Pine – Its Role in Stilbenoid Biosynthesis –

  A. Kodan; H. Kuroda; F. Sakai

  The Third International Wood Science Symposium, 02 Nov. 2001, Invited, Lead author

  
• アカマツのＣ末端配列欠失ピノシルビン合成酵素の性質

  小段篤史; 黒田宏之; 酒井富久美

  第51回日本木材学会大会, Apr. 2001, Lead author

  
• 大腸菌で発現させたアカマツ（Pinus densiflora）のスチルベン合成酵素

  小段篤史; 黒田宏之; 酒井富久美

  日本農芸化学大会, Mar. 2001, Lead author

  
• 大腸菌で発現させた３種類のスチルベン合成酵素の性質

  小段篤史; 黒田宏之; 酒井富久美

  植物細胞分子生物学大会, Jul. 2000, Lead author

  
• アカマツのスチルベン合成酵素 –大腸菌発現系による同定と性質解明-

  小段篤史; 黒田宏之; 酒井富久美

  第50回日本木材学会大会, Apr. 2000, Lead author

  
• アカマツのスチルベン合成酵素の大腸菌での発現

  小段篤史; 黒田宏之; 酒井富久美

  日本農芸化学大会, Apr. 2000, Lead author

  
• スチルベン合成酵素遺伝子の大腸菌における発現とその生育阻害

  小段篤史; 黒田宏之; 酒井富久美

  第49回日本木材学会大会, Apr. 1999, Lead author

  
• アカマツのスチルベン合成酵素群

  小段篤史; 黒田宏之; 酒井富久美

  日本農芸化学大会, Apr. 1999, Lead author

  
• スチルベン合成酵素遺伝子プローブによるアカマツ芽生えcDNAライブラリーのスクリーニング

  小段篤史; 黒田宏之; 酒井富久美

  第48回日本木材学会大会, Apr. 1998, Lead author

  
• アカマツのポリケチド合成酵素のcDNAクローニング

  小段篤史; 黒田宏之; 酒井富久美

  日本農芸化学大会, Apr. 1998, Lead author

  
• イヌの 脳におけるメタロチオネイン遺伝子の発現とその加齢における意義

  小嶋聖; 小段篤史; 小林欣滋; 森田剛仁; 島田章則; 山野好章; 梅村孝司

  第124 回日本 獣医学会, Oct. 1997

  

Presentations

• Direct Visualization of ABCA1 Reconstituted in Nanodisc by High-Speed Atomic Force Microscopy (HS-AFM)

  Atsushi Kodan; Romain Amyo; Yasuhisa Kimura; Noriyuki Kodera; Kazumitsu Ueda

  ABC2023, the 9th FEBS Special Meeting “ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Disease” Innsbruck, Austria, 01 Mar. 2023

  
• Cryo-EM解析を目指した脂質輸送型ABC蛋白質の生産・調製基盤の構築

  小段篤史; 光岡薫; 木村泰久; 木岡紀幸; 植田和光

  日本農芸化学大会2021年度大会 （オンライン開催）, 20 Mar. 2021

  
• 多剤排出ポンプMDR1の薬剤絞り出しメカニズム

  小段篤史; 木村泰久; 植田和光

  第15回トランスポーター研究会年会 （オンライン開催）, 16 Oct. 2020, Invited

  
• Structural basis for gating mechanisms of a eukaryotic ABC transporter

  Atsushi Kodan

  WPI–iCeMS Industry Advisory Committee Conference, 14 Oct. 2014, Invited

  
• High-resolution crystal structure of an ABC multidrug exporter from Cyanidioschyzon merolae, a thermophilic unicellular eukaryote

  Atsushi Kodan

  ABC2014, the 5th FEBS Special Meeting “ATP-Binding Cassette Proteins: From Multidrug Resistance to Genetic Disease”, 08 Mar. 2014, Invited

  
• X-ray crystallographic analysis of an exporter-type ABC transporter.

  Atsushi Kodan

  4th FEBS Special Meeting on ABC Proteins - ABC2012, 03 Mar. 2012

  
• X-ray crystallographic analysis of an exporter-type ABC transporter

  Atsushi Kodan

  Kakenhi Kiban (S)/iCeMS Joint International Symposium “ABC2011 in Kyoto -ABC Proteins/Membrane Meso-domains/ES-iPS cells”, 11 Nov. 2011

  
• Stilbene Synthase from Japanese Red Pine – Its Role in Stilbenoid Biosynthesis

  A. Kodan; H. Kuroda; F. Sakai

  The Third International Wood Science Symposium, 02 Nov. 2001, Invited

  

Books and Other Publications

• BIOSCIENCE & INDUSTRY

  小段篤史; 植田和光, Joint work, 脂溶性化合物を“絞り出す”多剤排出ポンプMDR1の分子機構 74(4), 280-283.

  一般財団法人バイオインダストリー協会, Jul. 2021

  
• ファルマシア

  小段篤史; 木村泰久; 植田和光, Contributor, 多剤排出ポンプMDR1の薬剤絞り出し機構 56(6), 1-5.

  日本薬学会, 01 Jun. 2020

  
• 膜タンパク質工学ハンドブック

  監修 津本; 浩平; 浜窪; 隆雄 分担執筆; 木村泰久; 小段篤史; 植田和光, Contributor, 第2編膜タンパク質—医学・薬学への展開、第3章トランスポーター、第1節 多剤輸送体MDR1(ABCB1) p211-216

  エヌ・ティー・エス, Apr. 2020

  Find Kyoto University's collection of books :

  
• 未来材料

  小段篤史; 木村泰久; 加藤博章; 植田和光, Joint work, ABC (ATP-binding cassette) タンパク質の構造解析が拓く創薬の未来 6(10), 30-36

  エヌ・ティー・エス, 10 Oct. 2006, Not refereed

  
• ABC蛋白質

  編集 植田和光; 分担執筆 加藤博章、小段篤史、柴田洋之, Contributor, ABC蛋白質の立体構造研究—現状と課題 p245-274

  学会出版センター, May 2005

  Find Kyoto University's collection of books :

  

Media Coverage

• 京大、多剤排出ポンプが薬を輸送するメカニズムを解明

  日本経済新聞電子版, 10 Jan. 2019, Paper

  
• 薬効かない再発がん、仕組み解明

  読売新聞, 17面, 31 Mar. 2014, Paper

  
• 京大グループ、抗がん剤働きを阻害、原因タンパク質を解明、薬剤開発に期待

  京都新聞, 27面, 04 Mar. 2014, Paper

  
• 「京大と東大、がん化学療法の阻害たんぱく質の類似分子構造を解明」

  日刊工業新聞, 19面, 04 Mar. 2014, Paper

  

External funds: Kakenhi

• 細胞外基質による細胞機能制御機構の包括的理解とその応用基盤の研究

  Grant-in-Aid for Scientific Research (A)

  Medium-sized Section 38:Agricultural chemistry and related fields

  Kyoto University

  木岡 紀幸

  From 05 Apr. 2021, To 31 Mar. 2025, Granted

  接着斑;細胞外マトリックス;細胞分化;メカノバイオロジー;コラーゲン;細胞接着

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H04713/
• Deciphering the Molecular Structure and Functional Role for a Key Lipid Transport ABC protein

  Grant-in-Aid for Scientific Research (B)

  Basic Section 38030:Applied biochemistry-related

  Kyoto University

  Atsushi Kodan

  From 01 Apr. 2020, To 31 Mar. 2023, Project Closed

  脂質輸送型ABC蛋白質;新生HDL;善玉コレステロール;動脈硬化;輸送体;ATP;分子メカニズム;膜タンパク質;脂質輸送型ABCタンパク質;脂質代謝;アポA-I;ATP加水分解活性;脂質輸送;ABC蛋白質;立体構造;機能;分子機構;生体分子;膜蛋白質;機能ー構造相関;コレステロール;脂質;トランスポーター;細胞膜;構造

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20H02912/
• Structural Basis for drug transport by ABC transporters

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Atsushi Kodan

  From 01 Apr. 2017, To 31 Mar. 2020, Project Closed

  ABC輸送体;P糖蛋白質;膜蛋白質;分子メカニズム;機能-構造相関;ATP;生体分子;タンパク質;膜タンパク質;立体構造;機能;蛋白質;構造変化;バイオテクノロジー;薬理学;癌

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K07306/
• Elucidation of the molecular mechanism of ABC transporters

  Grant-in-Aid for Young Scientists (A)

  Kyoto University

  Atsushi KODAN

  From 01 Apr. 2012, To 31 Mar. 2015, Project Closed

  生体分子;膜タンパク質;癌;多剤耐性;立体構造解析;X線結晶構造解析;ABCタンパク質;P糖タンパク質;蛋白質;薬学

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24689029/
• X-ray crystallographic analysis of an ABC transporter

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  Atsushi KODAN

  From 01 Apr. 2010, To 31 Mar. 2012, Project Closed

  生体分子;蛋白質;薬学;癌;ABC蛋白質;X線結晶構造解析;創薬

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790512/
• Struchural biology of membrane protein trabsporters

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Hiroaki KATO

  From 01 Apr. 2005, To 31 Mar. 2008, Project Closed

  ABCトランスポーター;P糖タンパク質;X線結晶解析;単粒子構造解析;膜タンパク質;トランスポーター;構造生物学;電子顕微鏡;多剤耐性;胆汁排出;糖尿病, ABC transporter;P-glycoprotein;X-ray crystalloqraphy;Single Particle Analysis;membrane proteins

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380066/
• X線解析を目指した多剤耐性ポンプMDR1の大量発現系の構築と結晶化

  Grant-in-Aid for Exploratory Research

  Kyoto University

  加藤 博章

  From 01 Apr. 2004, To 31 Mar. 2006, Project Closed

  P糖タンパク質;多剤耐性;抗ガン剤;構造生物学;X線結晶学;構造薬理学;膜タンパク質;X線結晶解析;バキュロウイルス;MDR1;結晶化;昆虫細胞

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16659012/

External funds: others

• Structural basis for drug transport of ABC transporters

  京都大学コアステージバックアップ研究費

  From 01 Apr. 2015, To 31 Mar. 2016

  小段篤史
• Structure-based mechanism for recognition of ES cell specific probe KP-1 by MDR1 and ABCG2.

  京都大学iCeMS加速研究課題バックアップ研究費

  From 01 Apr. 2012, To 31 Mar. 2013

  小段篤史
• Structure-based mechanism for recognition of ES/iPS cell specific probe KP-1 by MDR1.

  京都大学iCeMS加速研究課題バックアップ研究費

  From 01 Apr. 2014, To 31 Mar. 2015

  小段篤史
• Atomic-resolution crystal structure-based molecular mechanism of a eukaryotic ABC multidrug transporter.

  京都大学iCeMS加速研究課題バックアップ研究費

  From 01 Apr. 2015, To 31 Mar. 2016

  小段篤史

Education

Teaching subject(s)

• From 01 Apr. 2025, To 31 Mar. 2026

  Experimental Course of Cellular Biochemistry

  CG01, Spring, Graduate School of Agriculture, 3
• From 01 Apr. 2025, To 31 Mar. 2026

  Experimental Course of Cellular Biochemistry

  CG01, Fall, Graduate School of Agriculture, 3
• From 01 Apr. 2025, To 31 Mar. 2026

  Outline of Physiological Chemistry

  X101, Fall, Graduate School of Agriculture, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Cellular Biochemistry Seminar

  CF01, Spring, Graduate School of Agriculture, 1.5
• From 01 Apr. 2024, To 31 Mar. 2025

  Cellular Biochemistry Seminar

  CF01, Fall, Graduate School of Agriculture, 1.5
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course of Cellular Biochemistry

  CG01, Spring, Graduate School of Agriculture, 3
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course of Cellular Biochemistry

  CG01, Fall, Graduate School of Agriculture, 3
• From 01 Apr. 2023, To 31 Mar. 2024

  Cellular Biochemistry Seminar2

  CB18, Year-long, Graduate School of Agriculture, 3
• From 01 Apr. 2023, To 31 Mar. 2024

  Cellular Biochemistry Seminar1

  CB17, Year-long, Graduate School of Agriculture, 3
• From 01 Apr. 2023, To 31 Mar. 2024

  Experimental Course of Cellular Biochemistry2

  CC18, Year-long, Graduate School of Agriculture, 6
• From 01 Apr. 2023, To 31 Mar. 2024

  Experimental Course of Cellular Biochemistry1

  CC17, Year-long, Graduate School of Agriculture, 6
• From 01 Apr. 2022, To 31 Mar. 2023

  Cellular Biochemistry Seminar2

  CB18, Year-long, Graduate School of Agriculture, 3
• From 01 Apr. 2022, To 31 Mar. 2023

  Cellular Biochemistry Seminar1

  CB17, Year-long, Graduate School of Agriculture, 3
• From 01 Apr. 2022, To 31 Mar. 2023

  Structural Biology

  5638, Spring, Faculty of Agriculture, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course of Cellular Biochemistry2

  CC18, Year-long, Graduate School of Agriculture, 6
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course of Cellular Biochemistry1

  CC17, Year-long, Graduate School of Agriculture, 6
• From Apr. 2015, To Mar. 2016

  Experimental Course of Cellular Biochemistry1

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Experimental Course of Cellular Biochemistry

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Cellular Biochemistry Seminar1

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Cellular Biochemistry Seminar

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Experimental Course of Cellular Biochemistry1

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Experimental Course of Cellular Biochemistry2

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Experimental Course of Cellular Biochemistry

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Cellular Biochemistry Seminar1

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Cellular Biochemistry Seminar2

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Cellular Biochemistry Seminar

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Experimental Course of Cellular Biochemistry1

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Experimental Course of Cellular Biochemistry2

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Experimental Course of Cellular Biochemistry

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Cellular Biochemistry Seminar1

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Cellular Biochemistry Seminar2

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Cellular Biochemistry Seminar

  Year-long, 農学研究科
• From Apr. 2018, To Mar. 2019

  Outline of Physiological Chemistry

  Fall, 全学共通科目
• From Apr. 2018, To Mar. 2019

  Experimental Course of Cellular Biochemistry1

  Year-long, 農学研究科
• From Apr. 2018, To Mar. 2019

  Experimental Course of Cellular Biochemistry2

  Year-long, 農学研究科
• From Apr. 2018, To Mar. 2019

  Cellular Biochemistry Seminar1

  Year-long, 農学研究科
• From Apr. 2018, To Mar. 2019

  Cellular Biochemistry Seminar2

  Year-long, 農学研究科
• From Apr. 2019, To Mar. 2020

  Experimental Course of Cellular Biochemistry1

  Year-long, 農学研究科
• From Apr. 2019, To Mar. 2020

  Experimental Course of Cellular Biochemistry2

  Year-long, 農学研究科
• From Apr. 2019, To Mar. 2020

  Cellular Biochemistry Seminar1

  Year-long, 農学研究科
• From Apr. 2019, To Mar. 2020

  Cellular Biochemistry Seminar2

  Year-long, 農学研究科
• From Apr. 2020, To Mar. 2021

  Structural Biology

  Spring, 農学部
• From Apr. 2020, To Mar. 2021

  Experimental Course of Cellular Biochemistry1

  Year-long, 農学研究科
• From Apr. 2020, To Mar. 2021

  Experimental Course of Cellular Biochemistry2

  Year-long, 農学研究科
• From Apr. 2020, To Mar. 2021

  Cellular Biochemistry Seminar1

  Year-long, 農学研究科
• From Apr. 2020, To Mar. 2021

  Cellular Biochemistry Seminar2

  Year-long, 農学研究科
• From Apr. 2021, To Mar. 2022

  Structural Biology

  Spring, 農学部
• From Apr. 2021, To Mar. 2022

  Experimental Course of Cellular Biochemistry1

  Year-long, 農学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course of Cellular Biochemistry2

  Year-long, 農学研究科
• From Apr. 2021, To Mar. 2022

  Cellular Biochemistry Seminar1

  Year-long, 農学研究科
• From Apr. 2021, To Mar. 2022

  Cellular Biochemistry Seminar2

  Year-long, 農学研究科
• From Apr. 2022, To Mar. 2023

  構造生物学

  Spring, 農学部
• From Apr. 2022, To Mar. 2023

  応用生命科学

  Year-long, 農学研究科

Academic, Social Contribution

Academic Contribution

• Nature Communications

  Peer review

  Springer Nature Ltd., From 21 Nov. 2023, To 06 Aug. 2024

  
• The Journal of Biochemistry

  Peer review

  The Japanese Biochemical Society, From 14 Jan. 2020, To 29 Jan. 2020

  

Social Contribution

• 京都大学iCeMSサイエンスフェスティバル2019

  Guest, Panelist, Logistic support, Performer

  京都大学, 京都大学iCeMSサイエンスフェスティバル2019, 京都大学iCeMS, From 08 Nov. 2019, To 08 Nov. 2019