Basic Information

Affiliated programs (koza)

• Graduate School of Science, 化学専攻 生物化学講座放射線生命化学分科, 准教授

Professional Memberships

• 水晶体研究会
• International Society of Eye Research
• Association for Research in Vision and Ophthalmology
• THE JAPANESE SOCIETY FOR CATARACT RESEARCH
• THE JAPANESE BIOCHEMICAL SOCIETY
• D-AMINO ACID RESEARCH SOCIETY

Academic Degree

• 修士（理学）(京都大学)
• 博士（理学）(京都大学)

Research History

• From Mar. 2025, To Present

  Kyoto University, Kyoto University Institute for Integrated Radiation and Nuclear Science, 教授
• From Apr. 2021, To Present

  JST創発的研究支援事業
• From Apr. 2020, To Feb. 2025

  Kyoto University, Kyoto University Institute for Integrated Radiation and Nuclear Science, 准教授
• From Apr. 2018, To Mar. 2020

  Kyoto University Institute for Integrated Radiation and Nuclear Science, 放射線生命科学研究部門 基礎老化研究部門, 特定准教授
• From Apr. 2017, To Mar. 2018

  Kyoto University, Research Reactor Institute, Division of Radiation Life Science, 特定准教授
• From Sep. 2016, To Mar. 2017

  KYoto University, 助教
• From Apr. 2015, To Aug. 2016

  Tokyo University of Pharmacy and Life Sciences, 助教
• From Oct. 2013, To Mar. 2015

  Kyoto University, Research Reactor Institute, 博士研究員
• From Oct. 2009, To Sep. 2013

  Massachusetts Institute of Technology, Post doc associate
• From Apr. 2006, To Sep. 2009

  Oregon Health and Science University,, Post doc associate

ID,URL

• J-Global ID

  201401030522702123

• Researcher number

  80379007

researchmap URL

• https://researchmap.jp/7000010668

Research

Research Topics, Overview of the research

• Research Topics

  Advanced research for D-amino acid residues in protein

• Overview of the research

  Proteins consist exclusively of L-amino acids; nonetheless, D-aspartic acid (D-Asp) has been detected in various human tissue from elderly individuals. We have developed original D/L analysis systems and identified many isomerization sites in proteins, while pathogenesis has been still obscure. The formation and contribution of D-Asp residues in proteins would be one of significant factors of age-related disease. The goal of our study is to observe actual physiological effect of D-Asp formation in protein, and relate D-Asp residues with age-related diseases.

Research Interests

• クリスタリン
• ヒートショックプロテイン
• シャペロン
• 電磁波
• 水晶体
• 加齢性疾患
• フォールディング
• D-アミノ酸

Research Areas

• Life sciences, Nutrition and health science, aging
• Life sciences, Structural biochemistry, D-amino acid

Papers

• The Importance of the Idea of 'Parachirality' in Life Science

  Noriko Fujii; Takumi Takata; Hiroaki Sakaue

  Advances in Asymmetric Autocatalysis and Related Topics, 23 May 2017

  
• Characterization of different-sized human αA-crystallin homomers and implications to Asp151 isomerization

  Jiayue Sun; Toshiya Matsubara; Tamaki Koide; Kirsten Lampi; Larry David; Takumi Takata

  PLOS ONE, Jul. 2024, Peer-reviewed, Corresponding author

  
• Characterization of βB2-crystallin tryptophan mutants reveals two different folding states in solution

  Jiayue Sun; Ken Morishima; Rintaro Inoue; Masaaki Sugiyama; Takumi Taka

  Protein Science, Jun. 2024, Peer-reviewed, Corresponding author

  
• Identification of D-amino acid residues in proteins using mass spectrometry

  髙田 匠

  BUNSEKI KAGAKU, Jun. 2022, Peer-reviewed, Invited, Lead author, Corresponding author

  Access this paper :

  
• Eye lens β-crystallins are predicted by native ion mobility-mass spectrometry and computations to form compact higher-ordered heterooligomers.

  Amber D Rolland; Takumi Takata; Micah T Donor; Kirsten J Lampi; James S Prell

  Structure (London, England : 1993), 11 Jul. 2023

  
• Characterization of K-binding factor involved in water-soluble complex of menaquinone-7 produced by Bacillus subtilis natto.

  Toshiyuki Chatake; Yasuhide Yanagisawa; Risa Murakami; Tadanori Ohsugi; Hiroyuki Sumi; Takumi Takata; Aya Okuda; Ken Morishima; Rintaro Inoue; Masaaki Sugiyama

  Journal of biochemistry, 07 Jul. 2023

  
• Elucidation of the mechanism of subunit exchange in αB crystallin oligomers.

  Rintaro Inoue; Yusuke Sakamaki; Takumi Takata; Kathleen Wood; Ken Morishima; Nobuhiro Sato; Aya Okuda; Masahiro Shimizu; Reiko Urade; Noriko Fujii; Masaaki Sugiyama

  Scientific reports, 28 Jan. 2021

  Access this paper :

  
• Isomerization of Asp is essential for assembly of amyloid-like fibrils of αA-crystallin-derived peptide.

  Kosuke Magami; Naomi Hachiya; Kazuo Morikawa; Noriko Fujii; Takumi Takata

  PloS one, 2021

  
• Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2).

  Naoki Yamamoto; Shun Takeda; Natsuko Hatsusaka; Noriko Hiramatsu; Noriaki Nagai; Saori Deguchi; Yosuke Nakazawa; Takumi Takata; Sachiko Kodera; Akimasa Hirata; Eri Kubo; Hiroshi Sasaki

  Cells, 11 Dec. 2020

  
• Simultaneous and Rapid Detection of Multiple Epimers and Isomers of Aspartyl Residues in Lens Proteins Using an LC-MS-MRM Method.

  Noriko Fujii; Takumi Takata; Ingu Kim; Toshiya Matsubara

  ACS omega, 27 Oct. 2020

  
• Asp isomerization increases aggregation of α-crystallin and decreases its chaperone activity in human lens of various ages.

  Noriko Fujii; Takumi Takata; Ingu Kim; Ken Morishima; Rintaro Inoue; Kousuke Magami; Toshiya Matsubara; Masaaki Sugiyama; Tamaki Koide

  Biochimica et biophysica acta. Proteins and proteomics, Sep. 2020, Peer-reviewed

  
• Site-Specific Rapid Deamidation and Isomerization in Human Lens αA-crystallin in vitro.

  Takata T; Ha S; Koide T; Fujii N

  Protein Science, Apr. 2020, Peer-reviewed

  
• Negative charge at aspartate 151 is important for human lens αA-crystallin stability and chaperone function.

  Takata T; Matsubara T; Nakamura-Hirota T; Fujii N

  Experimental eye research, May 2019, Peer-reviewed

  
• D-Amino acids in protein: The mirror of life as a molecular index of aging

  Noriko Fujii; Takumi Takata; Norihiko Fujii; Kenzo Aki; Hiroaki Sakaue

  Biochimica et Biophysica Acta - Proteins and Proteomics, 01 Jul. 2018, Peer-reviewed, Invited

  
• Identification of Isomeric Aspartate residues in βB2-crystallin from Aged Human Lens

  Takumi Takata; Kento Murakami; Atsuhiko Toyama; Noriko Fujii

  Biochimica et Biophysica Acta - Proteins and Proteomics, 01 Jul. 2018, Peer-reviewed, Invited

  
• Asp 58 modulates lens αA-crystallin oligomer formation and chaperone function.

  Takata T; Nakamura-Hirota T; Inoue R; Morishima K; Sato N; Sugiyama M; Fujii N

  The FEBS journal, Apr. 2018, Peer-reviewed

  
• Identification of D-amino acid-containing peptides in human serum

  Seongmin Ha; Ingu Kim; Takumi Takata; Tadatoshi Kinouchi; Masaharu Isoyama; Minoru Suzuki; Noriko Fujii

  PLOS ONE, Dec. 2017, Peer-reviewed

  
• Isomeric Replacement of a Single Aspartic Acid Induces a Marked Change in Protein Function: The Example of Ribonuclease A

  Hiroaki Sakaue; Tadatoshi Kinouchi; Noriko Fujii; Norihiko Fujii; Takumi Takata

  ACS Omega, 31 Jan. 2017, Peer-reviewed

  
• The age related increase of D-amino acids and advanced glycation end-products in protein.

  Noriko Fujii; Takumi Takata

  Glycative Stress Research, 2017, Invited

  
• Ferulic Acid Suppresses Amyloid beta Production in the Human Lens Epithelial Cell Stimulated with Hydrogen Peroxide

  Noriaki Nagai; Sachiyo Kotani; Yu Mano; Akina Ueno; Yoshimasa Ito; Toshio Kitaba; Takumi Takata; Noriko Fujii

  BIOMED RESEARCH INTERNATIONAL, 2017, Peer-reviewed

  
• New insight into the dynamical system of alpha B-crystallin oligomers

  Rintaro Inoue; Takumi Takata; Norihiko Fujii; Kentaro Ishii; Susumu Uchiyama; Nobuhiro Sato; Yojiro Oba; Kathleen Wood; Koichi Kato; Noriko Fujii; Masaaki Sugiyama

  SCIENTIFIC REPORTS, Jul. 2016, Peer-reviewed

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• Aggregation of Trp > Glu point mutants of human gamma-D crystallin provides a model for hereditary or UV-induced cataract

  Eugene Serebryany; Takumi Takata; Erika Erickson; Nathaniel Schafheimer; Yongting Wang; Jonathan A. King

  PROTEIN SCIENCE, Jun. 2016, Peer-reviewed

  
• Isomerization of Asp residues plays an important role in A-crystallin dissociation

  Takumi Takata; Noriko Fujii

  FEBS JOURNAL, Mar. 2016, Peer-reviewed

  
• Isomerization of Asp residues plays an important role in αA-crystallin dissociation.

  Takata T; Fujii N

  The FEBS journal, Mar. 2016, Peer-reviewed

  
• D-amino acid residues in proteins related to aging and age-related diseases and a new analysis of the isomers in proteins

  Noriko Fujii; Takumi Takata; Norihiko Fujii; Kenzo Aki; Hiroaki Sakaue

  D-Amino Acids: Physiology, Metabolism, and Application, 01 Jan. 2016, Peer-reviewed

  
• Isomerization of aspartyl residues in crystallins and its influence upon cataract.

  Fujii N; Takata T; Fujii N; Aki K

  Biochimica et biophysica acta, Jan. 2016, Peer-reviewed

  
• Isomerization of aspartyl residues in crystallins and its influence upon cataract

  Noriko Fujii; Takumi Takata; Norihiko Fujii; Kenzo Aki

  BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, Jan. 2016, Peer-reviewed

  
• Quantitative analysis of isomeric (L-alpha-, L-beta-, D-alpha, D-beta-) aspartyl residues in proteins from elderly donors

  Noriko Fujii; Takumi Takata; Norihiko Fujii

  JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, Dec. 2015, Peer-reviewed

  
• Effect of Asp 96 isomerization on the properties of a lens alpha B-crystallin-derived short peptide

  Takumi Takata; Noriko Fujii

  JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, Dec. 2015, Peer-reviewed

  
• Alpha B- and beta A3-crystallins containing D-Aspartic acids exist in a monomeric state

  Hiroaki Sakaue; Takumi Takata; Norihiko Fujii; Hiroshi Sasaki; Noriko Fujii

  BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Jan. 2015, Peer-reviewed

  
• Alpha B- and βA3-crystallins containing d-aspartic acids exist in a monomeric state.

  Sakaue H; Takata T; Fujii N; Sasaki H; Fujii N

  Biochimica et biophysica acta, Jan. 2015, Peer-reviewed

  
• Rapid Survey of Four Asp Isomers in Disease-Related Proteins by LC-MS combined with Commercial Enzymes

  Hiroki Maeda; Takumi Takata; Norihiko Fujii; Hiroaki Sakaue; Satoru Nirasawa; Saori Takahashi; Hiroshi Sasaki; Noriko Fujii

  ANALYTICAL CHEMISTRY, Jan. 2015, Peer-reviewed

  
• Cataract Formation and Age-Dependent Post-Translational Modifications in Crystallin

  Takumi Takata; Noriko Fujii

  The Journal of the Japanese Society for Cataract Research, 2015, Invited

  
• The C-terminal cysteine annulus participates in auto-chaperone function for Salmonella phage P22 tailspike folding and assembly.

  Takata T; Haase-Pettingell C; King J

  Bacteriophage, Jan. 2012, Peer-reviewed

  
• Solvent accessibility of beta B2-crystallin and local structural changes due to deamidation at the dimer interface

  Takumi Takata; Joshua P. Smith; Brian Arbogast; Larry L. David; Kirsten J. Lampi

  EXPERIMENTAL EYE RESEARCH, Sep. 2010, Peer-reviewed

  
• Deamidation alters interactions of beta-crystallins in hetero-oligomers

  Takumi Takata; Luke G. Woodbury; Kirsten J. Lampi

  MOLECULAR VISION, Jan. 2009, Peer-reviewed

  
• Deamidation destabilizes and triggers aggregation of a lens protein, beta A3-crystallin

  Takumi Takata; Julie T. Oxford; Borries Demeler; Kirsten J. Lampi

  PROTEIN SCIENCE, Sep. 2008, Peer-reviewed

  
• Localization of D-beta-aspartic acid-containing proteins in human eyes

  Yuichi Kaji; Tetsuro Oshika; Yutaka Takazawa; Masashi Fukayama; Takumi Takata; Noriko Fujii

  INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Sep. 2007, Peer-reviewed

  
• Deamidation alters the structure and decreases the stability of human lens beta Alpha 3-crystallin

  Takumi Takata; Julie T. Oxford; Theodore R. Brandon; Kirsten J. Lampi

  BIOCHEMISTRY, Jul. 2007, Peer-reviewed

  
• Detection of the specific D-aspartic acid residues in protein

  N. Fujii; T. Takata

  D-Amino Acids: A New Frontier in Amino Acid and Protein Research - Practical Methods and Protocols, 2007, Peer-reviewed

  
• Differential analysis of D-beta-Asp-containing proteins found in normal and infrared irradiated rabbit lens

  T Takata; T Shimo-Oka; M Kojima; K Miki; N Fujii

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, May 2006, Peer-reviewed

  
• Characterization of new D-beta-aspartate-containing proteins in a lens-derived cell line

  T Takata; T Shimo-Oka; K Miki; N Fujii

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Sep. 2005, Peer-reviewed

  
• Comparison of post-translational modifications of alpha A-crystallin from normal and hereditary cataract rats

  N Fujii; N Takeuchi; N Fujii; T Tezuka; K Kuge; T Takata; A Kamei; T Saito

  AMINO ACIDS, Mar. 2004, Peer-reviewed

  
• Characterization of alpha A-crystallin from high molecular weight aggregates in the normal human lens

  N Fujii; M Awakura; L Takemoto; M Inomata; T Takata; N Fujii; T Saito

  MOLECULAR VISION, Jul. 2003, Peer-reviewed

  
• Immunological detection of D-beta-aspartate-containing protein in lens-derived cell lines

  DW Yang; N Fujii; T Takata; T Shimo-Oka; S Tajima; Y Tanaka; T Saito

  MOLECULAR VISION, May 2003, Peer-reviewed

  
• The presence of D-beta-aspartic acid-containing peptides in elastic fibers of sun-damaged skin: a potent marker for ultraviolet-induced skin aging

  N Fujii; S Tajima; N Tanaka; N Fujimoto; T Takata; T Shimo-Oka

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Jun. 2002, Peer-reviewed

  

Misc.

• EXPRESSION OF D-BETA-ASPARTATE CONTAINING PROTEIN IN LENS-DERlVED CELL LINE

  TAKATA Takumi

  Viva origino, 01 Mar. 2002

  
• CHARACTERIZATION OF NEW D-BETA-ASPARTATE CONTAINING PROTEINS IN A LENS-DERIVED CELL LINE(Chiral Homogeneity and D-Amino Acids, International Symposium on Origins of Life and Astrobiology) :

  Takata Takumi; Shimo-Oka Tadashi; Miki Kunio; Fujii Noriko

  Viva origino, 2005

  
• Joinning in the ISOLAB'05 in Niigata

  Takata Takumi

  Viva origino, 2005

  
• Views and Mini Reviews regarding the 31th annual meeting of the SSOEL-JAPAN

  Takata Takumi

  Viva origino, 2006

  
• 28 The Research About D-β-Asp Containing Protein in Lens(General Contribution,The 31th annual meeting of the SSOEL-JAPAN (Abstracts))

  Takata Takumi; Shimo-Oka Tadashi; Kinouchi Tadatoshi; Fujii Noriko

  Viva origino, 2006

  
• The spontaneous isomerization of aspartate residues in lens crystallin

  Takumi Takat; Noriko Fujii

  生化学, Jun. 2019, Invited

  
• Heat stability and chaperone like function of aged lens protein mimics

  Takumi Takata

  JASCO Report, 2019, Invited

  
• 蛋白質中のD-アミノ酸と老化

  藤井紀子; 高田匠; 金仁求

  臨床免疫・アレルギー科, 2018, Invited

  
• 水晶体構成蛋白質中におけるアミノ酸の自発的化学修飾が引き起こす白内障発症機構に関する研究

  Takumi Takata

  日本白内障学会誌, 2018, Invited

  
• 生命科学におけるパラキラリティーの重要性

  Noriko Fujii; Takumi Takata

  日本眼薬理, 2017, Invited

  
• 白内障と酸化ストレス

  Takumi Takata; Ingu KIm; Noriko Fujii

  OCULISTA, 2017, Invited

  
• D-アミノ酸と老化; 新規老化マーカーとしてのD-アミノ酸の有用性

  Noriko Fujii; Takumi Takata

  アンチエイジング医学-日本抗加齢学会雑誌, 2017, Invited

  
• Analysis of the Post-translational Modifications of the Individual Amino Acids in Lens Proteins which were induced by aging and irradiation

  藤井紀子; 金仁求; 齊藤毅; 高田匠

  放射線生物研究, 2017, Invited

  
• Age-related Asp Isomerizations in Dissociated Alpha-crystallin from Aged Lens

  Takumi Takata; Takashi Sato; Noriko Fujii

  INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Sep. 2016

  
• A New Rapid Analysis of Amino Acid Isomers in Crystallins of Cataract Lenses: Toward Prevention and Cure of Cataract

  Fujii Noriko; Takata Takumi; Fujii Norihiko; Kim Ingu

  The Journal of The Japanese Society for Cataract Research, 2016, Invited

  
• 加齢によるタンパク質ホモキラリティーの破れ

  Noriko Fujii; Takumi Takata; Hiroaki Sakaue; Norihiko Fujii

  Viva Origino, 2016, Invited

  
• KINETICS OF SUBUNITS IN α-CRYSTALLIN AS STUDIED BY SMALL-ANGLE NEUTRON SCATTERING

  R. Inoue; T. Takata; N. Fujii; N. Sato; Y. Oba; S. Takata; N. Fujii; M. Sugiyama

  16th International Conference on Small-Angle Scattering, Sep. 2015

  
• 1 Oxidation of amino acid residues of γ-crystallin induced by γ-irradiation(General Session,The 39^ Annual Meeting of the SSOEL-Japan Program)

  Kim Ingu; Takata Takumi; Saito Takeshi; Fujii Norihiko; Kanamoto Takashi; Fujii Noriko

  Viva origino, 2014

  
• Characterization of D-β-Asp containing protein in N/N1003A ce 11 line

  TAKATA Takumi; FUJII Noriko; TANAKA Yoshiko; SHIMOOKA Masashi

  Viva origino, 30 Mar. 2003

  

Presentations

• ヒト水晶体内部シャペロン様分子αB-Crystallin中において部位特異的に修飾を受けるAsp部位の重要性

  佐々木 大輔; 守島 健; 井上 倫太郎; 杉山 正明; 高田 匠

  第97回 日本生化学会, 07 Nov. 2024

  
• Oxidation models of βB2-crystallin and their insights of cataract formation

  Jiayue Sun; Ken Morishima; Rintaro Inoue; Masaaki Sugiyama; Takumi Takata

  第97回 日本生化学会, 07 Nov. 2024

  
• ヒト水晶体内部シャペロン様分子αB-Crystallin中において部位特異的に修飾を受けるAsp部位の重要性

  佐々木 大輔; 守島 健; 井上 倫太郎; 杉山 正明; 高田 匠

  第97回 日本生化学会, 06 Nov. 2024

  
• X線小角散乱法を用いたβB2-クリスタリン酸化モデルフォールディング経路の解析

  孫 嘉玥; 守島 健; 井上 倫太郎; 杉山 正明; 高田 匠

  第63回白内障学会総会、第50回水晶体研究会, 23 Aug. 2024

  
• Analysis of the Susceptible Tryptophan Residues on the Greek Key Motifs of βB2-Crystallin

  Jiayue Sun; Takumi Takata

  9th International Conference on the Lens, 12 Dec. 2023

  
• The site-specific impact of modifications of aspartyl residues on lens αB-crystallin

  Daisuke Sasaki; Takumi Takata

  9th International Conference on the Lens, 11 Dec. 2023

  
• 加齢性または先天性白内障モデルβB２-クリスタリン変異体Foldingにおける温度依存性

  髙田 匠

  第62回日本白内障学会総会／第49回水晶体研究会, 22 Jul. 2023, Invited

  
• βB2-クリスタリン中のGreek key motif内部トリプトファン残基の重要性

  孫 嘉玥; 加賀澤 悠太; 髙田 匠

  第62回日本白内障学会・第49回水晶体研究会, 21 Jul. 2023

  
• Rapid deamidation/isomerization of Asparagine residues and effect of C-terminal adjacent amino acid residues in human lens αA-crystallin

  Takumi Takata

  XXVth biennial meeting of the International Society of Eye Research, 22 Feb. 2023, Invited

  
• 加齢に応じて進行する蛋白質中アミノ酸残基異性化に関する分離分析手法の現状

  高田匠

  第 50 回構造活性相関シンポジウム, 10 Nov. 2022, Invited

  
• 水晶体構成タンパク質中アミノ酸残基の傷と白内障

  高田 匠

  第61回日本白内障学会・第48回水晶体研究会, 28 Aug. 2022, Invited

  
• βB2-クリスタリン中151番目のトリプトファン残基の重要性

  加賀澤悠太; 高田匠

  第61回日本白内障学会・第48回水晶体研究会, 28 Aug. 2022

  
• Effect of C-terminal adjacent amino acid residues on deamidation/isomerization of asparagine residues in lens component proteins

  Takumi Takata

  Fifth International Conference on D-Amino Acid Research, 27 Jul. 2022, Invited

  
• βB1-クリスタリン中のアスパラギン酸残基の異性化

  加賀澤 悠太; 金 仁求; 高田 匠

  第60回日本白内障学会総会／第47回水晶体研究会, 26 Nov. 2021

  
• 水晶体構成蛋白質中アスパラギン酸残基異性化に際するC末端側隣接アミノ酸残基の影響

  菅河 晴菜; 髙田 匠

  第60回日本白内障学会総会／第47回水晶体研究会, 26 Nov. 2021

  
• Asp残基異性化に応じたα-クリスタリン由来ペプチドの機能及び構造の変化

  高田 匠; 眞上晃輔

  第60回日本白内障学会総会・第47回水晶体研究会合同大会, 26 Nov. 2021

  
• Oligomer structure and formation kinetics of cataract-associated proteins revealed with native ion mobility-mass spectrometry

  Amber D Rolland; Takumi Takata; Micah T. Donor; Samuel G. Wheeler; Larry L. David; Kirsten J. Lampi; James S. Prell

  69th American Society for Mass Spectrometry, 01 Nov. 2021

  
• たんぱく質の中にある年輪—新しい老化の指標について—

  高田 匠

  アトムサイエンスフェア講演会2021, 24 Oct. 2021, Invited

  
• Creation and evaluation of model proteins in which the isomerization proceeds rapidly in vitro

  高田 匠

  第16回 D-アミノ酸学会学術講演会, 11 Sep. 2021

  
• Subunit dynamics in alpha-crystallin through deuteration-assisted small-angle neutron scattering

  Inoue, Rintaro; Sakamaki, Yusuke; Takata, Takumi; Morishima, Ken; Wood, Kathleen; Sato, Nobuhiro; Okuda, Aya; Shimizu, Masahiro; Urade, Reiko; Fujii, Noriko; Sugiyama, Masaaki

  XXV General Assembly and Congress of the International Union of Crystallography - IUCr 2021, 16 Aug. 2021

  
• The Effects of Isomerization and Racemization of Asp Residues for Polypeptide Properties

  T. Takata; N. Hachiya; N. Fujii

  第43回日本分子生物学会年会, Dec. 2020, Invited

  
• 蛋白質結合型のアスパラギン酸残基の異性化修飾について

  高田匠; 藤井紀子

  第71回日本電気泳動学会総会, Nov. 2020, Invited

  
• クリスタリンの凝集とアスパラギン酸残基の異性化

  藤井紀子; 高田匠; 金仁求; 松原稔哉

  第59回日本白内障学会総会・第46回水晶体研究会合同大会, May 2020

  
• αA-クリスタリン中のAsp151をAsnに置換した組換え体では、同部位の異性化が10倍速く進行する

  高田 匠; 藤井 紀子

  第59回日本白内障学会総会・第46回水晶体研究会合同学会, May 2020

  
• クリスタリン内部アミノ酸の翻訳後修飾からみる水晶体老化

  高田匠

  第59回日本白内障学会総会・第46回水晶体研究会合同大会, May 2020, Invited

  
• Creation and analysis of isomers-containing lens αA-crystallin

  Takumi Takata; Ken Morishima; Rintaro Inoue; Masaaki Sugiyama; Noriko Fujii

  The 6th International Conference on the Lens 2019, 08 Dec. 2019, Invited

  
• D-amino acid in life science Origin, Aging and Universe

  Takumi Takata; Noriko Fujii

  Kyoto University International Symposium on Advanced Future Studies 2019, 23 Oct. 2019, Invited

  
• CREATION OF ASP-ISOMER-CONTAINING LENS aA-CRYSTALLIN USING THE EXPRESSED PROTEIN LIGATION

  Takumi Takata; Hiroaki Sakaue; Noriko Fujii

  The 4th International Conference of D-Amino Acid Research, 09 Sep. 2019, Invited

  
• 多重反応モニタリング(MRM)法を用いた老化蛋白質中のD-アミノ酸分析

  藤井紀子; 高田匠; 金仁求; 松原稔哉

  日本プロテオーム学会2019年大会 第70回日本電気泳動学会総会, 24 Jul. 2019

  
• クリスタリン中の複数箇所で見られるAsp異性体の一斉分析

  藤井紀子; 高田匠; 金仁求; 松原稔哉

  第58回日本白内障学会総会／第45回水晶体研究会, 19 Jul. 2019

  
• Asp151部位へAsp異性体を導入したαA-クリスタリンの作出

  高田 匠; 藤井 紀子

  第58回日本白内障学会総会・第45回水晶体研究会, 19 Jul. 2019

  
• Lens β­-crystallins preferentially form hetero­oligomers

  Lampi, Kirsten; Donor, Micah; Takata, Takumi; Vetter, Calvin; David, Larry; Prell, James

  The Association For Research in Vision and Ophthalmology 2019, 27 Apr. 2019

  
• D型アミノ酸で語る 新しい老化サイエンス

  高田 匠

  第14回京都大学附置研究所・センターシンポジウム, 09 Mar. 2019, Invited

  
• Asp76異性化による αA-crystallin由来ペプチドαA66-80の機能変化

  眞上晃輔; 木野内忠稔; 金仁求; 高田匠; 藤井紀子

  第14回D-アミノ酸学会, Sep. 2018

  
• クリスタリンの凝集とアスパラギン酸の異性化

  藤井紀子; 高田匠; 金仁求; 守島健; 井上倫太郎; 眞上晃輔; 杉山正明

  第14回D-アミノ酸学会, Sep. 2018

  
• The Mutation Studies for Isomerization Hot spots of Aspartate Residues in Lens αA-Crystallin

  T. Takata; N. Fujii

  XXIII Biennial Meeting of the International Society for Eye Research, Sep. 2018, Invited

  
• β-クリスタリン中のアスパラギン酸残基の異性化

  金 仁求; 高田 匠; 加治 優一; 藤井 紀子

  第14回D-アミノ酸学会, Sep. 2018

  
• ヒト水晶体内αA-クリスタリン中のAsp151残基異性化について

  高田匠; 藤井紀子

  第14回D-アミノ酸学会, Sep. 2018

  
• α-クリスタリンの凝集とアスパラギン酸残基の異性化

  藤井紀子; 高田匠; 金仁求; 守島健; 井上倫太郎; 眞上晃輔; 杉山正明

  第57回日本白内障学会総会・第44回水晶体研究会 合同大会, Jul. 2018

  
• ヒト水晶体内αA-クリスタリン中におけるAsp151部位の重要性

  高田匠; 藤井紀子

  第57回日本白内障学会総会・第44回水晶体研究会 合同大会, Jul. 2018

  
• Isomerization and racemization hot spots in lens αA-crystallin

  Takumi Takata; Noriko Fujii

  １８回日本蛋白質科学会年会, Jun. 2018

  
• The Site-specific Impact of Modifications of Aspartate Residues on Lens αA-Crystallin

  T. Takata; Rintaro Inoue; Ken Morishima; Nobuhiro Sato; Masaaki Sugiyama; Noriko Fujii

  The Association For Research in Vision and Ophthalmology, Apr. 2018

  
• The Site-specific Impact of Modifications of Aspartate Residues on Lens αA-Crystallin

  T. Takata; Rintaro Inoue; Ken Morishima; Nobuhiro Sato; Masaaki Sugiyama; Noriko Fujii

  The Association For Research in Vision and Ophthalmology, 2018

  
• α-クリスタリンの凝集とアスパラギン酸残基の異性化

  藤井紀子; 高田匠; 金仁求; 守島健; 井上倫太郎; 眞上晃輔; 杉山正明

  第57回日本白内障学会総会・第44回水晶体研究会 合同大会, 2018

  
• ヒト水晶体内αA-クリスタリン中におけるAsp151部位の重要性

  高田匠; 藤井紀子

  第57回日本白内障学会総会・第44回水晶体研究会 合同大会, 2018

  
• β-クリスタリン中のアスパラギン酸残基の異性化

  金仁求; 高田匠; 加治優一; 藤井紀子

  第14回D-アミノ酸学会, 2018

  
• The loss of chaperone activity of human lens αA-crystallin-derived peptide 66-80 is dramatically recovered by the replacement of Lβisomer at Asp76

  K. Magami; T. Takata; I. Kim; N. Fujii

  International Conference on the Lens 2017, Dec. 2017

  
• 水晶体構成タンパク質凝集に関与するαA-クリスタリン内のAsp部位

  高田匠; Kirsten Lampi; 藤井紀子

  第10回タンパク質異常凝集専門研究会, Dec. 2017

  
• ヒト血清中のD-アミノ酸含有ペプチド同定

  Seongmin H; 金仁求; 高田匠; 藤井紀子

  第90回 日本生化学会 （ConBio2017）, Dec. 2017

  
• αA-クリスタリンの部分ペプチドを用いたAsp58の異性化速度に関するｐＨの影響

  石原和樹; 金仁求; 高田匠; 藤井紀子

  第90回 日本生化学会 （ConBio2017）, Dec. 2017

  
• The function of αA-crystallin peptide 66-80 is dramatically changed by Asp76 isomerization

  眞上晃輔; 高田匠; 金仁求; 木野内忠稔; 藤井紀子

  第90回 日本生化学会 （ConBio2017）, Dec. 2017

  
• Age-related modification of Asp58 and Asp151 in lens αA-crystallin contributes to cataract formation

  Takumi Takata; Noriko Fujii

  第90回 日本生化学会 （ConBio2017）, Dec. 2017, Invited

  
• LC-MS/MSによる白内障クリスタリンの翻訳後修飾の一斉分析

  金仁求; 高田匠; 加治優一; 藤井紀子

  第56回日本白内障学会総会・第43回水晶体研究会 合同大会, Aug. 2017

  
• alphaA-クリスタリン相互作用能に関与するAsp異性化

  高田匠; 金仁求; 藤井紀子

  第56回日本白内障学会総会・第43回水晶体研究会 合同大会, Aug. 2017

  
• 水晶体構成タンパク質中のアミノ酸の翻訳後修飾が引き起こす白内障発祥の機構解明に関する研究

  高田匠

  第56回日本白内障学会総会・第43回水晶体研究会 合同大会, Aug. 2017, Invited

  
• Analysis of the post-translational modifications of the individual amino acids in lens proteins which were induced by irradiation and aging

  Ingu Kim; Takumi Takata; Noriko Fujii

  The 3rd International Conference of D-Amino Acid Research, Jul. 2017

  
• The mechanism of D-amino acid formation in proteins and new method of protein-bound D-amino acid detection

  Noriko Fujii; Takumi Takata; Norihiko Fujii; Kenzo Aki; Horoaki Sakaue

  The 3rd International Conference of D-Amino Acid Research, Jul. 2017, Invited

  
• Age-related abnormal Asp 58 isomers distribution in lens specific αA-crystallin monomeric state

  Takumi Takata; Noriko Fujii

  The 3rd International Conference of D-Amino Acid Research, Jul. 2017, Invited

  
• Biological uncommon D-amino acids appear in various proteins during aging

  T. Takata

  Transdisciplinary Mie-Symposium 2017 “Origins and Evolutions of Complexity”, Jun. 2017, Invited

  
• 太陽紫外線による老人性白内障の進行とその発症機構の解明

  高田匠

  第65回UV/EB研究会, Jun. 2017, Invited

  
• アスパラギン酸の異性化が引き起こすタンパク質異常凝集と加齢性疾患について

  高田匠

  第二回D-アミノ酸ワークショップ, Feb. 2017, Invited

  
• アスパラギン酸の異性化が引き起こすタンパク質異常凝集と加齢性疾患について

  高田匠

  第二回D-アミノ酸ワークショップ, 2017, Invited

  
• Biological uncommon D-amino acids appear in various proteins during aging

  T. Takata

  Transdisciplinary Mie-Symposium 2017 “Origins and Evolutions of Complexity”, 2017, Invited

  
• 太陽紫外線による老人性白内障の進行とその発症機構の解明

  高田匠

  第65回UV/EB研究会, 2017, Invited

  
• Analysis of the post-translational modifications of the individual amino acids in lens proteins which were induced by irradiation and aging

  Ingu Kim; Takumi Takata; Noriko Fujii

  The 3rd International Conference of D-Amino Acid Research, 2017

  
• The mechanism of D-amino acid formation in proteins and new method of protein-bound D-amino acid detection

  Noriko Fujii; Takumi Takata; Norihiko Fujii; Kenzo Aki; Horoaki Sakaue

  The 3rd International Conference of D-Amino Acid Research, 2017, Invited

  
• Age-related abnormal Asp 58 isomers distribution in lens specific αA-crystallin monomeric state

  Takumi Takata; Noriko Fujii

  The 3rd International Conference of D-Amino Acid Research, 2017, Invited

  
• LC-MS/MSによる白内障クリスタリンの翻訳後修飾の一斉分析

  金仁求; 高田匠; 加治優一; 藤井紀子

  第56回日本白内障学会総会・第43回水晶体研究会 合同大会, 2017

  
• alphaA-クリスタリン相互作用能に関与するAsp異性化

  高田匠; 金仁求; 藤井紀子

  第56回日本白内障学会総会・第43回水晶体研究会 合同大会, 2017

  
• 水晶体構成タンパク質中のアミノ酸の翻訳後修飾が引き起こす白内障発祥の機構解明に関する研究

  高田匠

  第56回日本白内障学会総会・第43回水晶体研究会 合同大会, 2017, Invited

  
• 水晶体構成タンパク質凝集に関与するαA-クリスタリン内のAsp部位

  高田匠; Kirsten Lampi; 藤井紀子

  第10回タンパク質異常凝集専門研究会, 2017

  
• The loss of chaperone activity of human lens αA-crystallin-derived peptide 66-80 is dramatically recovered by the replacement of Lβisomer at Asp76

  K. Magami; T. Takata; I. Kim; N. Fujii

  International Conference on the Lens 2017, 2017

  
• ヒト血清中のD-アミノ酸含有ペプチド同定

  Seongmin Ha; 金仁求; 高田匠; 藤井紀子

  第90回 日本生化学会 （ConBio2017）, 2017

  
• αA-クリスタリンの部分ペプチドを用いたAsp58の異性化速度に関するｐＨの影響

  石原和樹; 金仁求; 高田匠; 藤井紀子

  第90回 日本生化学会 （ConBio2017）, 2017

  
• The function of αA-crystallin peptide 66-80 is dramatically changed by Asp76 isomerization

  眞上晃輔; 高田匠; 金仁求; 木野内忠稔; 藤井紀子

  第90回 日本生化学会 （ConBio2017）, 2017

  
• Age-related modification of Asp58 and Asp151 in lens αA-crystallin contributes to cataract formation

  Takumi Takata; Noriko Fujii

  第90回 日本生化学会 （ConBio2017）, 2017, Invited

  
• EGFR阻害剤によるざ瘡様皮疹における毛嚢内薬物のLC/MS解析

  高田匠; 秋元賀子; 水野晃治; 林伸和; 竹中祐子; 川島眞; 佐藤隆

  第115回日本皮膚科学会総会, Jun. 2016

  
• MEK and BRAF inhibitors augment the production and accumulation of sebum in hamster sebocytes

  T. Sato; T. Takata; T. Nebashi; S. Murakawa; K. Mizuno; N. Akimoto

  Society for Investigative Dermatology 2016 Annual Meeting, May 2016

  
• Age-related Asp Isomerizations in Dissociated Alpha-crystallin from Aged Lens

  T. Takata; S. Sato; N Fujii

  The Association For Research in Vision and Ophthalmology 2016, May 2016

  
• MEK and BRAF inhibitors augment the production and accumulation of sebum in hamster sebocytes

  T. Sato; T. Takata; T. Nebashi; S. Murakawa; K. Mizuno; N. Akimoto

  Society for Investigative Dermatology 2016 Annual Meeting, 2016

  
• Age-related Asp Isomerizations in Dissociated Alpha-crystallin from Aged Lens

  T. Takata; S. Sato; N Fujii

  The Association For Research in Vision and Ophthalmology 2016, 2016

  
• EGFR阻害剤によるざ瘡様皮疹における毛嚢内薬物のLC/MS解析

  高田匠; 秋元賀子; 水野晃治; 林伸和; 竹中祐子; 川島眞; 佐藤隆

  第115回日本皮膚科学会総会, 2016

  
• Age-related abnormal Asp Isomers distribution in lens specific αA-crystallin monomeric and polymeric state

  Takumi Takata; Takashi Sato; Hiroshi Sasaki; Noriko Fujii

  XXII Biennial Meeting of the International Society for Eye Research, 2016, Invited

  
• Age-dependent isomerization and racemization at specific aspartyl residues in lens crystallins: Analysis and biological relevance

  Noriko Fujii; Takumi Takata; Horoaki Sakaue; Hiroshi Sasaki

  XXII Biennial Meeting of the International Society for Eye Research, 2016, Invited

  
• 加齢による蛋白質ホモキラリテイ―の破れ

  藤井紀子; 高田匠; 坂上弘明; 藤井智彦

  第41回生命の起原および進化学会学術講演会, 2016, Invited

  
• RAFおよびMEK阻害剤によるざ瘡様皮疹の発症機構解明

  日本薬学会 第136年会, 2016

  
• ハムスター脂腺細胞におけるABCB1を介したPropionibacterium acnesによる皮脂分泌促進機構

  水野晃治; 秋元賀子; 高田匠; 佐藤隆

  第115回日本皮膚科学会総会, 2016

  
• 皮膚に対する近赤外線の生物活性評価

  秋元賀子; 田中志保; 田匠; 常深祐一郎; 川島眞; 佐藤隆

  第115回日本皮膚科学会総会, 2016

  
• EGFR阻害剤による_瘡様皮疹における毛嚢内薬物のLC/MS解析

  高田匠; 秋元賀子; 水野晃治; 林伸和; 竹中祐子; 川島眞; 佐藤隆

  第115回日本皮膚科学会総会, 2016

  
• ハムスター脂腺細胞におけるTLR2およびABCB1を介したP. acnesによる皮脂分泌促進機構

  水野晃治; 秋元賀子; 高田匠; 佐藤隆

  第41回日本香粧品学会, 2016

  
• 環状ホスファチジン酸によるヒト正常皮膚リンパ管内皮細胞管腔形成促進機構

  新田見恵祐; 水野晃治; 高田匠; 秋元賀子; 佐藤隆

  第48回日本結合組織学会学術大会, 2016

  
• 近赤外線研究の現状と今後の展望

  田中志保; 秋元賀子; 高田匠; 佐藤隆

  第8回近赤外線研究会, 2016

  
• EGFR阻害剤による_瘡様皮疹の発症機構：毛包内へ排出された薬剤による皮脂産生増強

  佐藤隆; 高田匠; 水野晃治; 林伸和; 竹中祐子; 川島眞; 秋元賀子

  第46回日本皮膚アレルギー・接触皮膚炎学会総会学術大会, 2016

  
• Isomerizations of aspartyl residues in lens crystallins from age-related cataracts

  N. Fujii; T. Takata; N. Fujii; H. Sasaki

  The Association For Research in Vision and Ophthalmology-ASIA, 2015

  
• The spontaneous breaking of homochirality in lens crystallins from elderly donors

  N. Fujii; N. Fuji; T. Takata; H. Sasaki

  International Conference on the Lens 2015, 2015, Invited

  
• Isomerization of Asp residues is different between monomer and hetero-polymer of alpha-crystallin in aged human lens.

  T. Takata; N Fujii

  International Conference on the Lens 2015, 2015, Invited

  
• KINETICS OF SUBUNITS IN α-CRYSTALLIN AS STUDIED BY SMALL-ANGLE NEUTRON SCATTERING

  R. Inoue; T. Takata; N. Fujii; N. Sato; Y. Oba; S. Tanaka; N. Fujii; M. Sugiyama

  16th International conference on Small-Angle Scattering 2015, 2015

  
• LC-MSによる結合型D-アミノ酸分析

  藤井紀子; 高田匠

  第40回 生命の起原および進化学会, 2015

  
• 結合型D-アミノ酸形成が誘起する水晶体蛋白質の不溶化

  高田匠; 藤井紀子

  第40回 生命の起原および進化学会, 2015

  
• 血液中のD-アミノ酸含有ペプチドの探索

  HA SEONGMIN; 高田匠; 藤井紀子

  第15 回日本蛋白質科学会年会, 2015

  
• 結合型D-アミノ酸が引き起こす水晶体クリスタリンの機能変化

  高田匠; 藤井紀子

  第11回D-アミノ酸学会, 2015, Invited

  
• 加齢後のヒト水晶体内におけるαA-クリスタリン単量体内と会合体内のAsp異性化率の相違

  高田匠; 藤井紀子

  第11回D-アミノ酸学会, 2015

  
• LC-MS/MSによる老化蛋白質中のD-アミノ酸一斉分析

  藤井紀子; 田匠; 藤井智彦

  第11回D-アミノ酸学会, 2015

  
• 眼内水晶体に対し紫外線および近赤外線が及ぼす影響

  高田匠; 藤井紀子; 佐藤隆

  第6回近赤外線研究会, 2015

  
• LC-MSによる白内障クリスタリン中の異性体アミノ酸の迅速分析法

  藤井紀子; 藤井智彦; 高田匠; 前田裕貴

  第54回日本白内障学会総会・第41回水晶体研究会 合同大会, 2015

  
• Deamidations In β-crystallin Family Are Highly Correlated With Lens Insolubulization During Aging

  T. Takata; N. Fujii; K. Lampi

  第14回蛋白質化学会, 2014

  
• ANALYSIS OF ASPARTYL ISOMERS IN SKIN PROTEIN BY LC-MS/MS

  Y. Suzuki; T. Takata; H. Sakaue; N Fujii

  The 2nd International Conference of D-Amino Acid Research, 2014

  
• A NOVEL RAPID COMPREHENSIVE ANALYSIS OF D-AMINO ACIDS IN CATARACT LENS PROTEINS USING LC-MS/MS

  N. Fujii; N. Fujii; T. Takata; H. Maeda; H. Sakaue

  The 2nd International Conference of D-Amino Acid Research, 2014, Invited

  
• ASP ISOMERIZATION IN EYE LENS DERIVED PEPTIDE ASSOCIATED WITH PEPTIDE-PEPTIDE AND PEPTIDE-PROTEIN INTERACTIONS

  T. Takata; H Sakaue; N Fujii

  The 2nd International Conference of D-Amino Acid Research, 2014, Invited

  
• INVERSION OF ASPARTYL RESIDUE DISTURBS ASSEMBLY OF CRYSTALLIN IN HUMAN CATARACT LENS

  H. Sakaue; T. Takata; N. Fujii; H Sasaki; N Fujii

  The 2nd International Conference of D-Amino Acid Research, 2014

  
• 脱アミド化が引き起こすβB1-βA3クリスタリン分子間相互作用変化

  高田匠; Kirsten Lampi; 藤井紀子

  第40回水晶体研究会, 2014

  
• βB2-クリスタリンC末端Asn残基異性化が引き起こす同一分子間相互作用変化

  西岡紀理; 藤井智彦; 高田匠; 藤井紀子

  第40回水晶体研究会, 2014

  
• ラット水晶体蛋白質に対するγ線照射の影響

  金仁求; 高田匠; 藤井智彦; 藤井紀子

  第40回水晶体研究会, 2014

  
• アスパラギン酸残基の異性化によるクリスタリン会合体の解離

  藤井紀子; 坂上弘明; 高田匠; 藤井智彦; 佐々木洋

  第53回日本白内障学会総会 「白内障基礎研究の最前線」招待シンポジウム, 2014

  
• 白内障とクリスタリンの翻訳後修飾

  高田匠; 藤井紀子

  第53回日本白内障学会総会 「白内障基礎研究の最前線」招待シンポジウム, 2014, Invited

  
• 放射線照射によるγ-クリスタリン中のアミノ酸残基の酸化

  金仁求; 高田匠; 齊藤剛; 藤井智彦; 藤井紀子

  第87回 日本生化学会, 2014

  
• Asp異性化がポリペプチド会合体へ及ぼす影響

  高田匠; 藤井紀子

  第87回 日本生化学会, 2014

  
• アスパラギン酸残基のD-体化による水晶体蛋白質の解離

  藤井紀子; 坂上弘明; 高田匠; 藤井智彦

  第7回タンパク質異常凝集専門研究会, 2014

  
• アスパラギン酸残基の異性化が誘起する水晶体部分ポリペプチドの凝集

  高田匠; 藤井紀子

  第7回タンパク質異常凝集専門研究会, 2014

  
• 脱アミド化が引き起こす水晶体タンパク質の凝集と白内障との関係

  高田匠; Kirsten Lampi; 藤井紀子

  第6回タンパク質異常凝集専門研究会, 2013

  
• Identification of S-Thiolation Sites in Lens Nuclei of O2-Treated Guinea Pigs Using MudPIT Analysis

  M. F. Simpanya; F. J. Giblin; G. Wistow; P. Wilmarth; L. J. Robertson; T. Takata; K. J. Lampi; L. L. David

  The Association For Research in Vision and Ophthalmology, 2009

  
• Amide Hydrogen Exchange Study for Local Structural Alteration in βΑ3-crystallin」The Association For Research in Vision and Ophthalmology

  T. Takata; K. J. Lampi

  The Association For Research in Vision and Ophthalmology, 2009

  
• Deamidations in βA3-crystallin Are Highly Correlated With Insolubilization During Aging

  T. Takata; K. J. Lampi

  The Association For Research in Vision and Ophthalmology, 2008

  
• Deamidation of βA3-crystallin prevents β-subunit exchange

  K. J. Lampi; T. Takata

  18th International Congress of Eye Research, 2008

  
• Deamidation at the Dimer Interface Destabilizes βA3-Crystallin

  T. Takata; K. J. Lampi

  The Association For Research in Vision and Ophthalmology, 2007

  
• 水晶体中のD-β-アスパラギン酸含有蛋白質の発現に関する研究

  高田匠; 下岡正志; 木野内忠稔; 藤井紀子

  第31回生命の起原および進化学会, 2006

  
• 赤外線照射後の水晶体中のD-β-型アスパラギン酸含有タンパク質の網羅的検索

  高田匠; 下岡正志; 三木邦夫; 藤井紀子

  第14回蛋白質化学会, 2006

  
• CHARACTERIZATION OF NEW D-BETA-ASPARTATE CONTAINING PROTEINS IN A LENS-DERIVED CELL LINE

  T. Takata; T. Shimo-Oka; K. Miki; N. Fujii

  International Symposium on Origins of Life and Astrobiology, 2005

  
• MALDI-TOF-MSによるウサギ水晶体上皮由来培養細胞中の新規D型アミノ酸（D-β-型アスパラギン酸）含有タンパク質の同定

  高田匠; 下岡正志; 三木邦夫; 藤井紀子

  第78回 日本生化学会, 2005

  
• 水晶体上皮由来培養細胞中のD-β-アスパラギン酸含有タンパクの同定

  高田匠; 藤井紀子; Dawei Yang; 下岡正志; 田中愛子

  第1回 D-アミノ酸研究会, 2004

  
• Lens培養細胞中のD-beta-Asp 含有タンパクの発現およびその性質

  高田匠; 藤井紀子; Dawei Yang; 下岡正志; 田中愛子

  第28回生命の起源および進化学会, 2003

  
• 水晶体培養細胞中のD-β-Asp含有タンパク質について

  高田匠; Dawei Yang; 下岡正志; 田中愛子; 藤井紀子

  第76回 日本生化学会, 2003

  
• 老化ストレスによる水晶体培養細胞中のD-beta-アスパラギン酸の発現量変化について

  高田匠; Dawei Yang; 下岡正志; 田中愛子; 藤井紀子

  第28回水晶体研究会, 2002

  
• 水晶体培養細胞中のD-beta-アスパラギン酸の発現について

  高田匠; 藤井紀子; Dawei Yang; 下岡正志; 田中愛子

  第27回生命の起源および進化学会, 2002

  
• 老化と水晶体培養細胞中のD-beta-アスパラギン酸の発現について

  高田匠; Dawei Yang; 下岡正志; 田中愛子; 藤井紀子

  第75回 日本生化学会, 2002

  
• EXPRESSION OF D-BETA-ASPARTATE IN LENS-DERIVED CELL LINE

  T. Takata; Y. Dawei; N. Fujii; Y. Tanaka

  US-Japan 2001 Cooperative Cataract Research Group Meeting, 2001

  
• 水晶体上皮由来培養細胞中のD-beta-アスパラギン酸の発現

  高田匠; Dawei Yang; 下岡正志; 田中愛子; 藤井紀子

  第74回 日本生化学会, 2001

  
• Deamidation Induces Local Structural Changes in BetaB2 and BetaA3-Crystallins That Disrupt Stabilizing Interactions With Other Crystallins

  K. Lampi; M. Michiel; T. Takata; E. Duprat; F. Skouri-Panet; S. Finet; A. Tardieu

  The Association For Research in Vision and Ophthalmology

  

Books and Other Publications

• The Importance of the Idea of“Parachirality” in Life Science.

  Noriko Fujii; Norihiko Fujii; Takumi Takata; Hiroaki Sakaue, ?Chapter 6, ?gADVANCES IN ASYMMETRIC AUTOCATALYSIS AND RELATED TOPICS

  Elsevier Inc, 2017, Not refereed

  Find Kyoto University's collection of books :

  
• D-Amino acids: Physiology, Metabolism, and Application

  Noriko Fujii; Takumi Takata; Norihiko Fujii; Kenzo Aki; Hiroaki Sakaue, D-Amino acid residues in proteins related to aging and age-related diseases and a new analysis of the isomers in proteins.

  Springer, 2016, Not refereed

  Find Kyoto University's collection of books :

  
• 皮膚蛋白質中のアスパラギン酸残基異性体と紫外線による皮膚老化の関連性

  藤井紀子; 高田匠

  シーエムシー出版, 2015, Not refereed

  
• D-Amino Acids: A New Frontier in Amino Acid and Protein Rsearch-practical Methods and Protocol's

  Fujii N; Takata T, Detection of the specific D-aspartic acid residues in protein.

  Nova Science Publishers, New York, 2007, Not refereed

  

Awards

• 2017

  日本白内障学会, 日本白内障学会　学術賞
• 2016

  日本皮膚科学会, 第115回日本皮膚科学会総会ポスター賞
• 2015

  D-アミノ酸学会, 第4回 D-アミノ酸学会奨励賞
• 2014

  水晶体研究会, 第40回水晶体研究会・セッション優秀賞

External funds: Kakenhi

• Innovative Research Visualizing Aging in Protein Molecules

  Grant-in-Aid for Scientific Research (C)

  Basic Section 59040:Nutrition science and health science-related

  Kyoto University

  Takumi Takata

  From 01 Apr. 2020, To 31 Mar. 2023, Project Closed

  クリスタリン;白内障;アスパラギン酸;翻訳後修飾;異性化;加齢;質量分析;MRM法;Asp異性化;抗体試薬;結合型D-Asp;D-アミノ酸;老化;水晶体;プロテオミクス

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K11646/
• 加齢に応じてタンパク質中に蓄積する異性化アミノ酸残基の生理的意義を解明する研究

  Fund for the Promotion of Joint International Research (Fostering Joint International Research (A))

  Kyoto University

  高田 匠

  From 01 Apr. 2018, To 31 Mar. 2019, Declined

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18KK0460/
• Isomerization of Aspartate residue as a new indicator of aging

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  Takumi Takata

  From 01 Apr. 2017, To 31 Mar. 2021, Project Closed

  クリスタリン;白内障;アスパラギン酸;翻訳後修飾;異性化;加齢;質量分析;MRM法;Asp異性化;結合型D-アミノ酸;D-アミノ酸;脱アミド化;加齢性疾患;タンパク質異常凝集;老化;水晶体;プロテオーム

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K13221/
• Age-related Asp Isomerizations in Lens Alpha-crystallin

  Grant-in-Aid for Young Scientists (B)

  Kyoto University;Tokyo University of Pharmacy and Life Science

  Takumi Takata

  From 01 Apr. 2015, To 31 Mar. 2017, Project Closed

  D-アミノ酸;クリスタリン;白内障;加齢;タンパク質異常凝集;質量分析;D-Asp;水晶体;crystallin;タンパク質

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K16514/
• 蛋白質中トリプトファン残基の段階的酸化を標的とした新しい光老化科学の基盤研究

  Grant-in-Aid for Scientific Research (C)

  Basic Section 59040:Nutrition science and health science-related

  Kyoto University

  高田 匠

  From 01 Apr. 2023, To 31 Mar. 2026, Granted

  クリスタリン;白内障;トリプトファン;酸化

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K10868/

External funds: others

• 蛋白質中D-アミノ酸を基盤とした未知生命科学研究領域の開拓

  JST 創発的研究支援事業

  From 01 Apr. 2021

  髙田匠
• A study using in vivo homochirality breakdown as a new index of aging

  武田科学振興財団

  高田匠

Education

Teaching subject(s)

• From 01 Apr. 2024, To 31 Mar. 2025

  Radiation and Humanity

  U132, Spring, Institute for Liberal Arts and Sciences, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar on Radiation Chemistry IC

  7138, Spring, Graduate School of Science, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar on Radiation Chemistry IB

  7137, Fall, Graduate School of Science, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar on Radiation Chemistry IA

  7136, Spring, Graduate School of Science, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar on Radiation Chemistry ID

  7139, Fall, Graduate School of Science, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Radiation Chemistry IC

  7242, Spring, Graduate School of Science, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Radiation Chemistry IB

  7241, Fall, Graduate School of Science, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Radiation Chemistry IA

  7240, Spring, Graduate School of Science, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Radiation Chemistry ID

  7243, Fall, Graduate School of Science, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Biochemistry IV

  4614, Spring, Faculty of Science, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Radiation Biochemistry II

  7013, Fall, Graduate School of Science, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Radiation and Humanity

  U132, Spring, Institute for Liberal Arts and Sciences, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Radiation Chemistry IA

  7136, Spring, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Radiation Chemistry ID

  7139, Fall, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Radiation Chemistry IC

  7138, Spring, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Radiation Chemistry IB

  7137, Fall, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Advanced Radiation Chemistry IA

  7240, Spring, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Advanced Radiation Chemistry ID

  7243, Fall, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Advanced Radiation Chemistry IC

  7242, Spring, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Advanced Radiation Chemistry IB

  7241, Fall, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Biochemistry IV

  4614, Spring, Faculty of Science, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Radiation Biochemistry I

  7012, Spring, Graduate School of Science, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Radiation Chemistry IA

  7240, Spring, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Radiation Chemistry IA

  7136, Spring, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Radiation Chemistry IB

  7241, Fall, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Radiation Chemistry ID

  7243, Fall, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Radiation and Man

  U132, Spring, Institute for Liberal Arts and Sciences, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Radiation Chemistry IB

  7137, Fall, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Biochemistry IV

  4614, Spring, Faculty of Science, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Radiation Chemistry ID

  7139, Fall, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Radiation Chemistry IC

  7138, Spring, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Radiation Biochemistry II

  7013, Fall, Graduate School of Science, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Radiation Chemistry IC

  7242, Spring, Graduate School of Science, 1
• From Apr. 2020, To Mar. 2021

  Radiation Biochemistry II

  Fall, 理学研究科
• From Apr. 2020, To Mar. 2021

  Radiation Biochemistry I

  Spring, 理学研究科
• From Apr. 2020, To Mar. 2021

  Seminar on Radiation Chemistry IA

  Spring, 理学研究科
• From Apr. 2020, To Mar. 2021

  Seminar on Radiation Chemistry IB

  Fall, 理学研究科
• From Apr. 2020, To Mar. 2021

  Seminar on Radiation Chemistry IC

  Spring, 理学研究科
• From Apr. 2020, To Mar. 2021

  Seminar on Radiation Chemistry ID

  Fall, 理学研究科
• From Apr. 2020, To Mar. 2021

  Advanced Radiation Chemistry IA

  Spring, 理学研究科
• From Apr. 2020, To Mar. 2021

  Advanced Radiation Chemistry IB

  Fall, 理学研究科
• From Apr. 2020, To Mar. 2021

  Advanced Radiation Chemistry IC

  Spring, 理学研究科
• From Apr. 2020, To Mar. 2021

  Advanced Radiation Chemistry ID

  Fall, 理学研究科
• From Apr. 2021, To Mar. 2022

  Radiation Biochemistry I

  Spring, 理学研究科
• From Apr. 2021, To Mar. 2022

  Seminar on Radiation Chemistry IA

  Spring, 理学研究科
• From Apr. 2021, To Mar. 2022

  Seminar on Radiation Chemistry IB

  Fall, 理学研究科
• From Apr. 2021, To Mar. 2022

  Seminar on Radiation Chemistry IC

  Spring, 理学研究科
• From Apr. 2021, To Mar. 2022

  Seminar on Radiation Chemistry ID

  Fall, 理学研究科
• From Apr. 2021, To Mar. 2022

  Advanced Radiation Chemistry IA

  Spring, 理学研究科
• From Apr. 2021, To Mar. 2022

  Advanced Radiation Chemistry IB

  Fall, 理学研究科
• From Apr. 2021, To Mar. 2022

  Advanced Radiation Chemistry IC

  Spring, 理学研究科
• From Apr. 2021, To Mar. 2022

  Advanced Radiation Chemistry ID

  Fall, 理学研究科

Part-time lecturer

• From 01 Apr. 2022, To 31 Mar. 2023

  生物化学Ⅳ

  通年, 京都大学, 理学部

Administration

Faculty management (title, position)

• From 01 Jun. 2022

  量子ビーム生体高分子統合研究センター センター員
• From 01 Apr. 2024

  学術公開委員会委員
• From 01 Jan. 2022, To 31 Dec. 2025

  内部監査委員会委員
• From 01 Apr. 2023

  人権委員会委員
• From 01 Apr. 2023, To 31 Mar. 2024

  出版チーム員

Academic, Social Contribution

Committee Memberships

• From Dec. 2021, To Present

  運営委員, D-アミノ酸学会
• From Jul. 2019, To Present

  評議員, 日本白内障学会
• From Aug. 2018, To Present

  編集委員, 日本白内障学会
• From 2018, To Present

  世話人, 水晶体研究会