Murai, Masatoshi(Graduate School of Agriculture, Division of Applied Life Sciences) | Activity Database on Education and Research, Kyoto University

Murai, Masatoshi

Graduate School of Agriculture, Division of Applied Life Sciences Associate Professor

Last Updated :2025/04/24

Basic Information

Faculty

農学部

Professional Memberships

Japan Bioenergetics Group

Pesticide Science Society of Japan

Japan Society for Bioscience, Biotechnology, and Agrochemistry

Academic Degree

修士（農学）(京都大学)

博士（農学）(京都大学)

Academic Resume (Undergraduate School/Majors)

京都大学, 農学部生物機能科学科, 卒業

High School

High School
滋賀県立彦根東高等学校

Research History

From Aug. 2018, To Present
Kyoto University, Graduate School of Agriculture, Associate Professor

From Apr. 2009, To Jul. 2018
Kyoto University, Graduate School of Agriculture, Assistant Professor

From Apr. 2007, To Mar. 2009
Japan Society for the Promotion of Science, JSPS research fellow (DC2)

Profile

Profile
Mitochondria are organelles that play an important role in oxidative phosphorylation (ATP production) in eukaryotic cells. They contain a series of respiratory chain complexes, ATP synthase, and membrane transporters, whose structural and functional insights have remained elusive. We are investigating the structure and function of such membrane proteins, which are intimately involved in energy metabolism, through chemical biology approaches.

Current research themes are as follows;
1. Exploring the inhibitor-binding site in mitochondrial NADH-quinone oxidoreductase (complex I).
2. Identification of the quinone/inhibitor-binding site in various redox enzymes involving in energy metabolism.
3. Screening and mode of action study of the chemicals targeting mitochondrial membrane transporters.

Language of Instruction

English

Last Updated :2025/04/24

Research

Research Topics, Overview of the research

Research Topics
1. Exploring the inhibitor-binding site in mitochondrial NADH-quinone oxidoreductase (complex I).
2. Identification of the quinone/inhibitor-binding site in various redox enzymes involving in energy metabolism.
3. Screening and mode of action study of the chemicals targeting mitochondrial membrane transporters.
Overview of the research
Mitochondria are organelles that play an important role in oxidative phosphorylation (ATP production) in eukaryotic cells. They contain a series of respiratory chain complexes, ATP synthase, and membrane transporters, whose structural and functional insights have remained elusive. We are investigating the structure and function of such membrane proteins, which are intimately involved in energy metabolism, through chemical biology approaches.

Research Interests

Chemical Biology

Respiratory complex I

Ubiquinone

Photoaffinity labeling

Organic Synthesis

Respiratory chain

Bioorganic chemistry

Biological organic chemistry

Research Areas

Life sciences, Bioorganic chemistry

Papers

Structural Elucidation of the Mechanism for Inhibitor Resistance in the Na+-Translocating NADH-Ubiquinone Oxidoreductase from Vibrio cholerae
Moe Ishikawa-Fukuda; Jun-ichi Kishikawa; Takahiro Masuya; Takeshi Ito; Nicole L. Butler; Danielle McFee; Takayuki Kato; Blanca Barquera; Hideto Miyoshi; Masatoshi Murai
Biochemistry, Apr. 2025, Peer-reviewed, Corresponding author

Pinpoint introduction of functional molecular probe into the NqrB subunit of Na+-translocating NADH-ubiquinone oxidoreductase from Vibrio cholerae
Saya Miyachi; Hinako Tanaka; Moe Ishikawa; Danielle Mcfee; Wataru Aoki; Masatoshi Murai; Blanca Barquera; Hideto Miyoshi; Takahiro Masuya
Biochimica et Biophysica Acta (BBA) - Bioenergetics, Mar. 2025, Peer-reviewed

Flometoquin, a novel insecticide, acts on mitochondrial complex III as a Qi inhibitor
Haruka Takeuchi; Masahiro Nomura; Ryo Horikoshi; Shu Aasano; Takaaki Miyake; Takeru Kobayashi; Masatoshi Murai; Ying-Ju Chen; Fergus Earley; John Mina; Andrew Crossthwaite
Journal of Pesticide Science, 2024, Peer-reviewed

Mitochondrial respiratory complex I can be inhibited via bypassing the ubiquinone-accessing tunnel
Ryohei Otani; Takahiro Masuya; Hideto Miyoshi; Masatoshi Murai
FEBS Letters, Jun. 2024, Peer-reviewed, Corresponding author

Identification of proteins involved in intracellular ubiquinone trafficking in Saccharomyces cerevisiae using artificial ubiquinone probe
Mirai Mizutani; Seina Kuroda; Masahide Oku; Wataru Aoki; Takahiro Masuya; Hideto Miyoshi; Masatoshi Murai
Biochimica et Biophysica Acta (BBA) - Bioenergetics, Jun. 2024, Peer-reviewed, Corresponding author

Respiratory complex I in mitochondrial membrane catalyzes oversized ubiquinones
Ryo Ikunishi; Ryohei Otani; Takahiro Masuya; Kyoko Shinzawa-Itoh; Tomoo Shiba; Masatoshi Murai; Hideto Miyoshi
Journal of Biological Chemistry, Jun. 2023, Peer-reviewed
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Discovery of Bis-sulfonamides as Novel Inhibitors of Mitochondrial NADH-Quinone Oxidoreductase (Complex I)
Atsuhito Tsuji; Takahiro Masuya; Norihito Arichi; Shinsuke Inuki; Masatoshi Murai; Hideto Miyoshi; Hiroaki Ohno
ACS Medicinal Chemistry Letters, 24 Jan. 2023, Peer-reviewed
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Cryo-EM structures of Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae
Jun-ichi Kishikawa; Moe Ishikawa; Takahiro Masuya; Masatoshi Murai; Yuki Kitazumi; Nicole L. Butler; Takayuki Kato; Blanca Barquera; Hideto Miyoshi
Nature Communications, Jul. 2022, Peer-reviewed
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Diverse reaction behaviors of artificial ubiquinones in mitochondrial respiratory complex I
Shinpei Uno; Takahiro Masuya; Oleksii Zdorevskyi; Ryo Ikunishi; Kyoko Shinzawa-Itoh; Jonathan Lasham; Vivek Sharma; Masatoshi Murai; Hideto Miyoshi
Journal of Biological Chemistry, May 2022, Peer-reviewed
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Cryo-electron microscopy reveals how acetogenins inhibit mitochondrial respiratory complex I
Daniel N. Grba; James N. Blaza; Hannah R. Bridges; Ahmed-Noor A. Agip; Zhan Yin; Masatoshi Murai; Hideto Miyoshi; Judy Hirst
Journal of Biological Chemistry, Mar. 2022, Peer-reviewed

Comprehensive understanding of multiple actions of anticancer drug tamoxifen in isolated mitochondria
Yufu Unten; Masatoshi Murai; Tomoki Koshitaka; Kotaro Kitao; Osamu Shirai; Takahiro Masuya; Hideto Miyoshi
Biochimica et Biophysica Acta (BBA) - Bioenergetics, Dec. 2021, Peer-reviewed

Natural tetramic acids elicit multiple inhibitory actions against mitochondrial machineries presiding over oxidative phosphorylation
Yufu Unten; Masatoshi Murai; Katsuyuki Sakai; Yukihiro Asami; Takenori Yamamoto; Takahiro Masuya; Hideto Miyoshi
Bioscience, Biotechnology, and Biochemistry, 08 Oct. 2021, Peer-reviewed, Corresponding author
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Traminines A and B, produced by Fusarium concentricum, inhibit oxidative phosphorylation in Saccharomyces cerevisiae mitochondria
Katsuyuki Sakai; Yufu Unten; Aoi Kimishima; Kenichi Nonaka; Takumi Chinen; Kazunari Sakai; Takeo Usui; Kazuro Shiomi; Masato Iwatsuki; Masatoshi Murai; Hideto Miyoshi; Yukihiro Asami; Satoshi Ōmura
Journal of Industrial Microbiology and Biotechnology, 03 Aug. 2021, Peer-reviewed

Specific chemical modification explores dynamic structure of the NqrB subunit in Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae
Moe Ishikawa; Takahiro Masuya; Hinako Tanaka; Wataru Aoki; Noam Hantman; Nicole L. Butler; Masatoshi Murai; Blanca Barquera; Hideto Miyoshi
Biochimica et Biophysica Acta (BBA) - Bioenergetics, Apr. 2021, Peer-reviewed

Pinpoint Dual Chemical Cross-Linking Explores the Structural Dynamics of the Ubiquinone Reaction Site in Mitochondrial Complex I
Takahiro Masuya; Shinpei Uno; Masatoshi Murai; Hideto Miyoshi
Biochemistry, 16 Mar. 2021, Peer-reviewed

Inhibitors of a Na+-pumping NADH-ubiquinone oxidoreductase play multiple roles to block enzyme function
Takahiro Masuya; Yuki Sano; Hinako Tanaka; Nicole L Butler; Takeshi Ito; Tatsuhiko Tosaki; Joel E. Morgan; Masatoshi Murai; Blanca Barquera; Hideto Miyoshi
Journal of Biological Chemistry, 20 Jul. 2020, Peer-reviewed
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Synthetic biology based construction of biological activity-related library of fungal decalin-containing diterpenoid pyrones.
Kento Tsukada; Shono Shinki; Akiho Kaneko; Kazuma Murakami; Kazuhiro Irie; Masatoshi Murai; Hideto Miyoshi; Shingo Dan; Kumi Kawaji; Hironori Hayashi; Eiichi N Kodama; Aki Hori; Emil Salim; Takayuki Kuraishi; Naoya Hirata; Yasunari Kanda; Teigo Asai
Nature communications, 14 Apr. 2020, Peer-reviewed
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IACS-010759, a potent inhibitor of glycolysis-deficient hypoxic tumor cells, inhibits mitochondrial respiratory complex I through a unique mechanism
Tsuji A; Akao T; Masuya T; Murai M; Miyoshi H
The Journal of Biological Chemistry, Apr. 2020, Peer-reviewed
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Exploring the binding pocket of quinone/inhibitors in mitochondrial respiratory complex I by chemical biology approaches
Murai, M
Biosci. Biotechnol. Biochem., Apr. 2020, Peer-reviewed, Invited, Lead author, Corresponding author
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Oversized ubiquinones as molecular probes for structural dynamics of the ubiquinone reaction site in mitochondrial respiratory complex I
Uno S; Masuya T; Shinzawa-Itoh K; Lasham J; Haapanen O; Shiba T; Inaoka DK; Sharma V; Murai M; Miyoshi H
The Journal of Biological Chemistry, Feb. 2020, Peer-reviewed

Photoaffinity labeling of respiratory complex I in bovine heart submitochondrial particles by photoreactive [125I]amilorides
Murai M; Miyoshi H
Bio-protocol, Sep. 2019, Peer-reviewed, Invited

Fusaramin, an antimitochondrial compound produced by Fusarium sp., discovered using multidrug-sensitive Saccharomyces cerevisiae.
Katsuyuki Sakai; Yufu Unten; Masato Iwatsuki; Hirotaka Matsuo; Wataru Fukasawa; Tomoyasu Hirose; Takumi Chinen; Kenichi Nonaka; Takuji Nakashima; Toshiaki Sunazuka; Takeo Usui; Masatoshi Murai; Hideto Miyoshi; Yukihiro Asami; Satoshi Ōmura; Kazuro Shiomi
The Journal of antibiotics, Sep. 2019, Peer-reviewed

The antibiotic korormicin A kills bacteria by producing reactive oxygen species
Maynard A; Butler N L; Ito T; da Silva A J; Murai M; Chen T; Koffas M A G; Miyoshi H; Barquera B
Journal of Bacteriology, May 2019, Peer-reviewed

Defining the mechanism of action of S1QELs, specific suppressors of superoxide production in the quinone-reaction site in mitochondrial complex I
Banba A; Tsuji A; Kimura H; Murai M; Miyoshi H
The Journal of Biological Chemistry, Apr. 2019, Peer-reviewed

Pentenediol-type compounds specifically bind to voltage-dependent anion channel 1 in Saccharomyces cerevisiae mitochondria
Unten Y; Murai M; Yamamoto T; Watanabe A; Ichimaru N; Aburaya S; Aoki W; Shinohara Y; Miyoshi H
Biochemistry, Feb. 2019, Peer-reviewed

Exploring the quinone/inhibitor-binding pocket in mitochondrial respiratory complex I by chemical biology approaches,
Uno S; Kimura H; Murai M; Miyoshi H
The Journal of Biological Chemistry, Jan. 2019, Peer-reviewed

Epoxycyclohexenedione-Type Compounds Make Up a New Class of Inhibitors of the Bovine Mitochondrial ADP/ATP Carrier
Ayaki Aoyama; Masatoshi Murai; Naoya Ichimaru; Shunsuke Aburaya; Wataru Aoki; Hideto Miyoshi
Biochemistry, 13 Feb. 2018, Peer-reviewed

Pinpoint Chemical Modification of the Quinone-Access Channel of Mitochondrial Complex I via a Two-Step Conjugation Reaction
Takahiro Masuya; Masatoshi Murai; Takeshi Ito; Shunsuke Aburaya; Wataru Aoki; Hideto Miyoshi
BIOCHEMISTRY, Aug. 2017, Peer-reviewed

Identification of the binding sites for ubiquinone and inhibitors in the Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae by photoaffinity labeling
Takeshi Ito; Masatoshi Murai; Satoshi Ninokura; Yuki Kitazumi; Katherine G. Mezic; Brady F. Cress; Mattheos A. G. Koffas; Joel E. Morgan; Blanca Barquera; Hideto Miyoshi
JOURNAL OF BIOLOGICAL CHEMISTRY, May 2017, Peer-reviewed

Characterization of the ubiquinone-binding protein Coq10 using a synthetic ubiquinone probe (in Japanese)
Murai M; Miyoshi H
Japanese Journal of Pesticide Science, Feb. 2017, Peer-reviewed, Invited

Action mechanism of the inhibitors of mitochondrial respiratory complex I based on structure-activity relationship (SAR) studies (in Japanese)
Miyoshi H; Murai M
Japanese Journal of Pesticide Science, Feb. 2017, Peer-reviewed, Invited

Synthetic Ubiquinones Specifically Bind to Mitochondrial Voltage-Dependent Anion Channel 1 (VDAC1) in Saccharomyces cerevisiae Mitochondria
Masatoshi Murai; Ayaka Okuda; Takenori Yamamoto; Yasuo Shinohara; Hideto Miyoshi
BIOCHEMISTRY, Jan. 2017, Peer-reviewed

Characterization of the reaction of decoupling ubiquinone with bovine mitochondrial respiratory complex I
Takahiro Masuya; Kenji Okuda; Masatoshi Murai; Hideto Miyoshi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Aug. 2016, Peer-reviewed

Current topics on inhibitors of respiratory complex I
Masatoshi Murai; Hideto Miyoshi
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Jul. 2016, Peer-reviewed

Specific Methylation of Asp160 (49 kDa subunit) Located inside the Quinone Binding Cavity of Bovine Mitochondrial Complex I
Masatoshi Murai; Hiroyuki Inaoka; Takahiro Masuya; Shunsuke Aburaya; Wataru Aoki; Hideto Miyoshi
BIOCHEMISTRY, Jun. 2016, Peer-reviewed

Current topics on inhibitors of respiratory complex I.
Murai M; Miyoshi H
Biochimica et biophysica acta, Jun. 2016, Peer-reviewed

Reduction of Synthetic Ubiquinone QT Catalyzed by Bovine Mitochondria! Complex I Is Decoupled from Proton Translocation
Kenji Okuda; Masatoshi Murai; Shunsuke Aburaya; Wataru Aoki; Hideto Miyoshi
BIOCHEMISTRY, Jan. 2016, Peer-reviewed

Ascosteroside C, a new mitochondrial respiration inhibitor discovered by pesticidal screening using recombinant Saccharomyces cerevisiae
Takuya Suga; Yukihiro Asami; Shohei Hashimoto; Kenichi Nonaka; Masato Iwatsuki; Takuji Nakashima; Ryohei Sugahara; Takahiro Shiotsuki; Takenori Yamamoto; Yasuo Shinohara; Naoya Ichimaru; Masatoshi Murai; Hideto Miyoshi; Satoshi Omura; Kazuro Shiomi
JOURNAL OF ANTIBIOTICS, Oct. 2015, Peer-reviewed

Production of new amilorides as potent inhibitors of mitochondrial respiratory complex I
Masatoshi Murai; Sayako Habu; Sonomi Murakami; Takeshi Ito; Hideto Miyoshi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Jul. 2015, Peer-reviewed

Identification of the Binding Position of Amilorides in the Quinone Binding Pocket of Mitochondrial Complex I
Takeshi Ito; Masatoshi Murai; Hironobu Morisaka; Hideto Miyoshi
BIOCHEMISTRY, Jun. 2015, Peer-reviewed

Amilorides Bind to the Quinone Binding Pocket of Bovine Mitochondrial Complex I
Masatoshi Murai; Sonomi Murakami; Takeshi Ito; Hideto Miyoshi
BIOCHEMISTRY, May 2015, Peer-reviewed

Trichopolyn VI: a new peptaibol insecticidal compound discovered using a recombinant Saccharomyces cerevisiae screening system
Takuya Suga; Yukihiro Asami; Shohei Hashimoto; Kenichi Nonaka; Masato Iwatsuki; Takuji Nakashima; Yoshihiro Watanabe; Ryohei Sugahara; Takahiro Shiotsuki; Takenori Yamamoto; Yasuo Shinohara; Naoya Ichimaru; Masatoshi Murai; Hideto Miyoshi; Satoshi Omura; Kazuro Shiomi
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2015, Peer-reviewed

Pinpoint Chemical Modification of Asp160 in the 49 kDa Subunit of Bovine Mitochondrial Complex I via a Combination of Ligand-Directed Tosyl Chemistry and Click Chemistry
Takahiro Masuya; Masatoshi Mural; Hironobu Morisaka; Hideto Miyoshi
BIOCHEMISTRY, Dec. 2014, Peer-reviewed

Chemical modifications of respiratory complex I for structural and functional studies
Masatoshi Murai; Hideto Miyoshi
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, Aug. 2014, Peer-reviewed

Identification of the Binding Site of the Quinone-Head Group in Mitochondrial Coq10 by Photoaffinity Labeling
Masatoshi Murai; Kohei Matsunobu; Sawako Kudo; Kentaro Ifuku; Makoto Kawamukai; Hideto Miyoshi
BIOCHEMISTRY, Jun. 2014, Peer-reviewed

Site-Specific Chemical Labeling of Mitochondrial Respiratory Complex I through Ligand-Directed Tosylate Chemistry
Takahiro Masuya; Masatoshi Murai; Kentaro Ifuku; Hironobu Morisaka; Hideto Miyoshi
BIOCHEMISTRY, Apr. 2014, Peer-reviewed

Fenpyroximate Binds to the Interface between PSST and 49 kDa Subunits in Mitochondrial NADH-Ubiquinone Oxidoreductase
Yusuke Shiraishi; Masatoshi Murai; Naoto Sakiyama; Kentaro Ifuku; Hideto Miyoshi
BIOCHEMISTRY, Mar. 2012, Peer-reviewed

Localization of Ubiquinone-8 in the Na+-pumping NADH: Quinone Oxidoreductase from Vibrio cholerae
Marco S. Casutt; Ruslan Nedielkov; Severin Wendelspiess; Sara Vossler; Uwe Gerken; Masatoshi Murai; Hideto Miyoshi; Heiko M. Moeller; Julia Steuber
JOURNAL OF BIOLOGICAL CHEMISTRY, Nov. 2011, Peer-reviewed

Bis-THF motif of acetogenin binds to the third matrix-side loop of ND1 subunit in mitochondrial NADH-ubiquinone oxidoreductase.
Nakanishi S; Abe M; Yamamoto S; Murai M; Miyoshi H
Biochimica et biophysica acta, Sep. 2011, Peer-reviewed

Bis-THF motif of acetogenin binds to the third matrix-side loop of ND1 subunit in mitochondrial NADH-ubiquinone oxidoreductase
Sayo Nakanishi; Masato Abe; Shuhei Yamamoto; Masatoshi Murai; Hideto Miyoshi
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Sep. 2011, Peer-reviewed

Exploring Interactions between the 49 kDa and ND1 Subunits in Mitochondrial NADH-Ubiquinone Oxidoreductase (Complex I) by Photoaffinity Labeling
Masatoshi Murai; Yuko Mashimo; Judy Hirst; Hideto Miyoshi
BIOCHEMISTRY, Aug. 2011, Peer-reviewed

Synthesis and characterization of photoaffinity probe of acetogenin, a strong inhibitor of mitochondrial complex I
Shuhei Yamamoto; Masato Abe; Sayo Nakanishi; Masatoshi Murai; Hideto Miyoshi
TETRAHEDRON LETTERS, Jun. 2011, Peer-reviewed

New insight into mechanism of ROS suppression in single subunit NADH-ibiquinone oxidoreductase (Ndi1) from Saccharomyces cerevisiae
YAMASHITA TETSUO; Yu Yang; Eiko Nakamaru-Ogiso; HASHIMOTO Takeshi; Masatoshi Murai; IGARASHI Junsuke; Hideto Miyoshi; Akemi Matsuno-Yagi; Takao Yagi; KOSAKA Hiroaki
The Journal of Physiological Sciences, Mar. 2011, Peer-reviewed

Reaction Mechanism of Single Subunit NADH-Ubiquinone Oxidoreductase (Ndi1) from Saccharomyces cerevisiae EVIDENCE FOR A TERNARY COMPLEX MECHANISM
Yu Yang; Tetsuo Yamashita; Eiko Nakamaru-Ogiso; Takeshi Hashimoto; Masatoshi Murai; Junsuke Igarashi; Hideto Miyoshi; Nozomu Mori; Akemi Matsuno-Yagi; Takao Yagi; Hiroaki Kosaka
JOURNAL OF BIOLOGICAL CHEMISTRY, Mar. 2011, Peer-reviewed

Probing the role of tryptophan-derived secondary metabolism in defense responses against Bipolaris oryzae infection in rice leaves by a suicide substrate of tryptophan decarboxylase
Atsushi Ishihara; Takahito Nakao; Yuko Mashimo; Masatoshi Murai; Naoya Ichimaru; Chihiro Tanaka; Hiromitsu Nakajima; Kyo Wakasa; Hisashi Miyagawa
PHYTOCHEMISTRY, Jan. 2011, Peer-reviewed

Exploring the Binding Site of Delta lac-Acetogenin in Bovine Heart Mitochondrial NADH-Ubiquinone Oxidoreductase
Nobuyuki Kakutani; Masatoshi Murai; Naoto Sakiyama; Hideto Miyoshi
BIOCHEMISTRY, Jun. 2010, Peer-reviewed

Complementation of coenzyme Q-deficient yeast by coenzyme Q analogues requires the isoprenoid side chain
Andrew M. James; Helena M. Cocheme; Masatoshi Murai; Hideto Miyoshi; Michael P. Murphy
FEBS JOURNAL, May 2010, Peer-reviewed

Concise procedure for the synthesis of cardiolipins having different fatty acid combinations
Masato Abe; Shigeki Kitsuda; Shunji Ohyama; Shinya Koubori; Masatoshi Murai; Hideto Miyoshi
TETRAHEDRON LETTERS, Apr. 2010, Peer-reviewed

Characterization of the Ubiquinone Binding Site in the Alternative NADH-Quinone Oxidoreductase of Saccharomyces cerevisiae by Photoaffinity Labeling
Masatoshi Murai; Tetsuo Yamashita; Mai Senoh; Yuko Mashimo; Michihiko Kataoka; Hiroaki Kosaka; Akemi Matsuno-Yagi; Takao Yagi; Hideto Miyoshi
BIOCHEMISTRY, Apr. 2010, Peer-reviewed

Exploring the binding site of acetogenin in the ND1 subunit of bovine mitochondrial complex I.
Sekiguchi K; Murai M; Miyoshi H
Biochimica et biophysica acta, Sep. 2009, Peer-reviewed

Exploring the binding site of acetogenin in the ND1 subunit of bovine mitochondrial complex I
Koji Sekiguchi; Masatoshi Murai; Hideto Miyoshi
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Sep. 2009, Peer-reviewed

Characterization of the Inhibitor Binding Site in Mitochondrial NADH-Ubiquinone Oxidoreductase by Photoaffinity Labeling Using a Quinazoline-Type Inhibitor
Masatoshi Murai; Koji Sekiguchi; Takaaki Nishioka; Hideto Miyoshi
BIOCHEMISTRY, Feb. 2009, Peer-reviewed

Synthesis and characterization of new piperazine-type inhibitors for mitochondrial NADH-ubiquinone oxidoreductase (complex I)
Naoya Ichimaru; Masatoshi Murai; Nobuyuki Kakutani; Junko Kako; Atsushi Ishihara; Yoshiaki Nakagawa; Takaaki Nishioka; Takao Yagi; Hideto Miyoshi
BIOCHEMISTRY, Oct. 2008, Peer-reviewed

Identification of receptors of main sex-pheromone components of three Lepidopteran species
Hidefumi Mitsuno; Takeshi Sakurai; Masatoshi Murai; Tetsuya Yasuda; Soichi Kugimiya; Rika Ozawa; Haruhiko Toyohara; Junji Takabayashi; Hideto Miyoshi; Takaaki Nishioka
EUROPEAN JOURNAL OF NEUROSCIENCE, Sep. 2008, Peer-reviewed

Dynamic function of the spacer region of acetogenins in the inhibition of bovine mitochondrial NADH-ubiquinone oxidoreductase (complex I)
Masato Abe; Akina Kubo; Shuhei Yamamoto; Yoshinori Hatoh; Masatoshi Murai; Yasunao Hattori; Hidefumi Makabe; Takaaki Nishioka; Hideto Miyoshi
BIOCHEMISTRY, Jun. 2008, Peer-reviewed

Crucial structural factors and mode of action of polyene Amides as inhibitors for mitochondrial NADH-Ubiquinone oxidoreductase (Complex I)
Takehiko Yoshida; Masatoshi Murai; Masato Abe; Naoya Ichimaru; Toshiyuki Harada; Takaaki Nishioka; Hideto Miyoshi
BIOCHEMISTRY, Sep. 2007, Peer-reviewed

Synthesis and Inhibitory Action of Novel Acetogenin Mimics Δlac-Acetogenins: A New Class of Inhibitors of Mitochondrial NADH-Ubiquinone Oxidoreductase (Complex-I)
Hideto Miyoshi; Naoya Ichimaru; Masatoshi Murai
Pesticide Chemistry: Crop Protection, Public Health, Environmental Safety, 31 May 2007, Peer-reviewed

The ND1 subunit constructs the inhibitor binding domain in bovine heart mitochondrial complex I
Masatoshi Murai; Atsushi Ishihara; Takaaki Nishioka; Takao Yagi; Hideto Miyoshi
BIOCHEMISTRY, May 2007, Peer-reviewed

Mode of inhibitory action of Delta lac-acetogenins, a new class of inhibitors of bovine heart mitochondrial complex I
Masatoshi Murai; Naoya Ichimaru; Masato Abe; Takaaki Nishioka; Hideto Miyoshi
BIOCHEMISTRY, Aug. 2006, Peer-reviewed

Mode of inhibitory action of Delta lac-acetogenins, a new class of inhibitors of bovine heart mitochondrial complex I
Masatoshi Murai; Naoya Ichimaru; Masato Abe; Takaaki Nishioka; Hideto Miyoshi
BIOCHEMISTRY, Aug. 2006, Peer-reviewed

Function of the alkyl side chains of Delta lac-acetogenins in the inhibitory effect on mitochondrial complex I (NADH-ubiquinone oxidoreductase)
N Ichimaru; M Abe; M Murai; M Senoh; T Nishioka; H Miyoshi
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, Jul. 2006, Peer-reviewed

Light control of mitochondrial complex I activity by a photoresponsive inhibitor
D Fujita; M Murai; T Nishioka; H Miyoshi
BIOCHEMISTRY, May 2006, Peer-reviewed

Synthesis of (4R,15R,16R,21S)- and (4R,15S,16S,21S)-rollicosin, squamostolide, and their inhibitory action with bovine heart mitochondrial complex I
H Makabe; Y Kimura; M Higuchi; H Konno; M Murai; H Miyoshi
BIOORGANIC & MEDICINAL CHEMISTRY, May 2006, Peer-reviewed

Synthesis of photolabile Delta lac-acetogenin for lphotoaffinity labeling of mitochondrial complex I
M Murai; N Ichimaru; M Abe; T Nishioka; H Miyoshi
JOURNAL OF PESTICIDE SCIENCE, 2006, Peer-reviewed

Mass spectrometric analysis of the ubiquinol-binding site in cytochrome bd from Escherichia coli
Y Matsumoto; M Murai; D Fujita; K Sakamoto; H Miyoshi; M Yoshida; T Mogi
JOURNAL OF BIOLOGICAL CHEMISTRY, Jan. 2006, Peer-reviewed

Dynamic function of the alkyl spacer of acetogenins in their inhibitory action with mitochondrial complex I (NADH-ubiquinone oxidoreductase)
M Abe; M Murai; N Ichimaru; A Kenmochi; T Yoshida; A Kubo; Y Kimura; A Moroda; H Makabe; T Nishioka; H Miyoshi
BIOCHEMISTRY, Nov. 2005, Peer-reviewed

Synthesis of non-THF analogs of acetogenin toward simplified mimics
D Fujita; N Ichimaru; M Abe; M Murai; T Hamada; T Nishioka; H Miyoshi
TETRAHEDRON LETTERS, Aug. 2005, Peer-reviewed

Synthesis of (4R,15R,16R,21S)- and (4R,15S,16S,21S)-rollicosin
H Makabe; M Higuchi; H Konno; M Murai; H Miyoshi
TETRAHEDRON LETTERS, Jul. 2005, Peer-reviewed

Synthesis and inhibition mechanism of Delta lac-acetogenins, a novel type of inhibitor of bovine heart mitochondrial complex I
N Ichimaru; M Murai; M Abe; T Hamada; Y Yamada; S Makino; T Nishioka; H Makabe; A Makino; T Kobayashi; H Miyoshi
BIOCHEMISTRY, Jan. 2005, Peer-reviewed

Synthesis and inhibition mechanism of Delta lac-acetogenins, a novel type of inhibitor of bovine heart mitochondrial complex I
N Ichimaru; M Murai; M Abe; T Hamada; Y Yamada; S Makino; T Nishioka; H Makabe; A Makino; T Kobayashi; H Miyoshi
BIOCHEMISTRY, Jan. 2005, Peer-reviewed

The side chain of ubiquinone plays a critical role in Na+ translocation by the NADH-ubiquinone oxidoreductase (Na+-NQR) from Vibrio cholerae
Moe Ishikawa; Takahiro Masuya; Seina Kuroda; Shinpei Uno; Nicole L. Butler; Sara Foreman; Masatoshi Murai; Blanca Barquera; Hideto Miyoshi
Biochimica et Biophysica Acta (BBA) - Bioenergetics, Mar. 2022, Peer-reviewed

Misc.

ミトコンドリア複合体-Iによる合成ユビキノンQTの還元はプロトン輸送と共役しない
奥田健司; 村井正俊; 油屋駿介; 青木航; 三芳秀人
日本農芸化学会大会講演要旨集(Web), 2016

トシル化学によるミトコンドリア複合体-Iのユビキノン結合部位の特異的メチル化
稲岡宏幸; 村井正俊; 桝谷貴洋; 油屋駿介; 青木航; 三芳秀人
日本農芸化学会大会講演要旨集(Web), 2016

ミトコンドリア複合体‐I阻害剤としてのアミロライド類の作用機構研究
MURAI MASATOSHI; MURAKAMI SONOMI; ITO TAKESHI; MIYOSHI HIDETO
日本農薬学会大会講演要旨集, 18 Mar. 2015

ミトコンドリア複合体‐Iのピンポイント化学修飾
MASUYA TAKAHIRO; MURAI MASATOSHI; MORISAKA HIRONOBU; MIYOSHI HIDETO
日本農薬学会大会講演要旨集, 18 Mar. 2015

出芽酵母ミトコンドリアVDACにおけるユビキノン結合部位の同定
OKUDA AYAKA; MURAI MASATOSHI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2015

アミロライド類縁体のミトコンドリア複合体‐Iにおける結合部位の同定
ITO TAKESHI; MURAI MASATOSHI; MORISAKA HIRONOBU; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2015

新規eptaibol化合物であるFKI‐6324Aは害虫AAC遺伝子組換え酵母の増殖を抑制する
SUGA TAKUYA; ASAMI YUKIHIRO; HASHIMOTO SHOHEI; IWATSUKI MASATO; NONAKA KEN'ICHI; NAKASHIMA TAKUJI; WATANABE YOSHIHIRO; MATSUMOTO ATSUKO; SUGAWARA RYOHEI; SHIOTSUKI TAKAHIRO; YAMAMOTO TAKENORI; SHINOHARA YASUO; ICHIMARU NAOYA; MURAI MASATOSHI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2015

ミトコンドリア複合体‐Iのピンポイント化学修飾
MASUYA TAKAHIRO; MURAI MASATOSHI; MORISAKA HIRONOBU; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2015

コレラ菌ナトリウム輸送型NADH‐ユビキノン酸化還元酵素(Na⁺‐NQR)におけるユビキノン結合部位の同定
NINOKURA SATOSHI; MURAI MASATOSHI; BARQUERA BLANCA; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2015

ミトコンドリア複合体‐Iのピンポイント化学修飾
MASUYA TAKAHIRO; MURAI MASATOSHI; MORISAKA HIRONOBU; MIYOSHI HIDETO
日本生体エネルギー研究会討論会講演要旨集, 11 Dec. 2014

ミトコンドリア複合体‐Iのメカニズム解明に向けた特異的化学修飾
MIYOSHI HIDETO; MASUTANI TAKAHIRO; MURAI MASATOSHI
生体膜と薬物の相互作用シンポジウム講演要旨集, 20 Nov. 2014

コエンザイムQ結合タンパク質Coq10の解析
KAWAMUKAI MAKOTO; SAI TOSHITADA; KAINO TOMOHIRO; MURAI MASATOSHI; MIYOSHI HIDETO
イソプレノイド研究会例会講演要旨集, 12 Sep. 2014

ミトコンドリア複合体‐Iの位置特異的化学修飾
MASUYA TAKAHIRO; MURAI MASATOSHI; MIYOSHI HIDETO
日本農薬学会大会講演要旨集, 13 Mar. 2014

ミトコンドリア複合体‐I阻害剤としてのアミロライド類縁体の合成と作用機構の解明
MURAKAMI SONOMI; ITO TAKESHI; HABU SAYAKO; MURAI MASATOSHI; MIYOSHI HIDETO
日本農薬学会大会講演要旨集, 13 Mar. 2014

ミトコンドリアNADH‐ユビキノン酸化還元酵素(複合体‐I)の部位特異的化学修飾
MASUYA TAKAHIRO; MURAI MASATOSHI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2014

キナゾリンのミトコンドリアADP/ATP輸送体における結合部位の同定
ASANO SHU; MURAI MASATOSHI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2014

ユビキノン結合性タンパク質Coq10におけるキノン結合部位の同定
MATSUNOBU KOHEI; MURAI MASATOSHI; KAWAMUKAI MAKOTO; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2014

病害虫ミトコンドリアに作用する天然物リガンドの探索
ASAMI YUKIHIRO; MORI MIHOKO; SUGA TAKUYA; IWATSUKI MASATO; NAKASHIMA TAKUJI; HASHIMOTO SHOHEI; NONAKA KEN'ICHI; MATSUMOTO ATSUKO; YUGE HIDETAKA; MASUMA ROKURO; TAKAHASHI YOKO; SUGAHARA RYOHEI; SHIOTSUKI TAKAHIRO; YAMAMOTO TAKENORI; SHINOHARA YASUO; ICHIMARU NAOYA; MURAI MASATOSHI; MIYOSHI HIDETO; OMURA SATOSHI; SHIOMI KAZURO
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2014

合成ユビキノンプローブを用いたミトコンドリアタンパク質Coq10の解析
MURAI MASATOSHI; MATSUNOBU KOHEI; KAWAMUKAI MAKOTO; MIYOSHI HIDETO
日本生体エネルギー研究会討論会講演要旨集, 18 Dec. 2013

ユビキノンプローブの合成とユビキノン結合性タンパク質Coq10の解析
MURAI MASATOSHI; MATSUNOBU KOHEI; KUDO SAWAKO; KAWAMUKAI MAKOTO; MIYOSHI HIDETO
日本農芸化学会関西支部講演会講演要旨集, 05 Sep. 2013

キナゾリン型阻害剤を用いたミトコンドリアNADH‐ユビキノン酸化還元酵素(複合体‐I)のドメイン間相互作用の解明
MURAI MASATOSHI; MASHIMO YUKO; HIRST JUDY; MIYOSHI HIDETO
日本農薬学会大会講演要旨集, 14 Mar. 2013

ユビキノンプローブの合成とユビキノン結合性タンパク質Coq10の解析
MATSUNOBU KOHEI; MURAI MASATOSHI; KUDO SAWAKO; KAWAMUKAI MAKOTO; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2013

ミトコンドリア複合体‐Iに作用する光反応性フェンピロキシメートの結合部位の同定
MURAI MASATOSHI; SHIRAISHI YUSUKE; SAKIYAMA NAOTO; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2013

ミトコンドリア複合体‐I阻害剤としてのアミロライド類縁体の合成と作用機構
HABU SAYAKO; MURAKAMI SONOMI; MURAI MASATOSHI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2013

ミトコンドリア複合体‐Iに作用するキナゾリン型阻害剤の結合部位の同定
ASANO SHU; MURAI MASATOSHI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2013

Characterization of the inhibitor-binding site in mitochondrial NADH-Ubiquinone oxidoreductase (complex I) using fenpyroximate analogues
M. Murai; Y. Shiraishi; N. Sakiyama; H. Miyoshi
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Oct. 2012

ミトコンドリア複合体‐Iの阻害剤結合部位の同定(2):結合部位の概観
MURAI MASATOSHI; SHIRAISHI YUSUKE; SAKIYAMA NAOTO; MIYOSHI HIDETO
日本農薬学会大会講演要旨集, 14 Mar. 2012

ミトコンドリア複合体‐Iの阻害剤結合部位の同定(1):フェンピロキシメートの結合部位
SHIRAISHI YUSUKE; MURAI MASATOSHI; SAKIYAMA NAOTO; MIYOSHI HIDETO
日本農薬学会大会講演要旨集, 14 Mar. 2012

ミトコンドリア複合体‐Iにおけるアセトゲニン結合部位の同定
NAKANISHI SAYO; ABE MASATO; MURAI MASATOSHI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2012

ユビキノンプローブの創製と出芽酵母におけるユビキノン結合性タンパク質のスクリーニング
KUDO SAWAKO; MURAI MASATOSHI; YAZAKI KAZUFUMI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集(Web), 05 Mar. 2012

ミトコンドリア複合体‐Iの阻害剤結合部位の同定
MURAI MASATOSHI; SHIRAISHI YUSUKE; SAKIYAMA NAOTO; MIYOSHI HIDETO
日本生体エネルギー研究会討論会講演要旨集, 2012

機能性ユビキノンプローブの合成と結合タンパク質の同定
DOI SAYAKO; MURAI MASATOSHI; YOKOYAMA MINORU; MIYOSHI HIDETO
日本農芸化学会関西支部講演会講演要旨集, 01 Oct. 2011

カルジオリピン類の合成によるシトクロムcとの分子間相互作用の解析
SHIMBAYASHI RYOTA; KOBORI SHIN'YA; MORIYAMA IKUKO; ABE MASATO; MURAI MASATOSHI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集, 05 Mar. 2011

ミトコンドリア複合体‐Iにおけるアセトゲニンの結合部位の同定
NAKANISHI SAYO; ABE MASATO; YAMAMOTO SHUHEI; MURAI MASATOSHI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集, 05 Mar. 2011

光親和性ユビキノンプローブの合成と標的タンパク質の解析
HABU SAYAKO; YOKOYAMA MINORU; MURAI MASATOSHI; ABE MASATO; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集, 05 Mar. 2011

ミトコンドリア複合体‐Iに作用する光反応性フェンピロキシメートの結合部位の同定
MURAI MASATOSHI; SAKIYAMA NAOTO; SHIRAISHI YUSUKE; ABE MASATO; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集, 05 Mar. 2011

ミトコンドリア複合体‐Iに作用する光反応性フェンピロキシメートの結合部位の同定(I)
MURAI MASATOSHI; SAKIYAMA NAOTO; SHIRAISHI YUSUKE; ABE MASATO; MIYOSHI HIDETO
日本農薬学会大会講演要旨集, 25 Feb. 2011

ミトコンドリア複合体‐Iに作用する光反応性フェンピロキシメートの結合部位の同定(II)
SAKIYAMA NAOTO; MURAI MASATOSHI; SHIRAISHI YUSUKE; ABE MASATO; MIYOSHI HIDETO
日本農薬学会大会講演要旨集, 25 Feb. 2011

光反応性アセトゲニンの合成と作用機構研究
ABE MASATO; NAKANISHI SAYO; YAMAMOTO SHUHEI; MURAI MASATOSHI; MIYOSHI HIDETO
日本農薬学会大会講演要旨集, 25 Feb. 2011

機能性ユビキノンプローブの合成とユビキノン結合性タンパク質の探索
YOKOYAMA MINORU; HABU SAYAKO; MURAI MASATOSHI; ABE MASATO; MIYOSHI HIDETO
日本農芸化学会関西支部講演会講演要旨集, 2011

キナゾリン型阻害剤を用いたミトコンドリアNADH‐ユビキノン酸化還元酵素(複合体‐I)のドメイン間相互作用の解明
MURAI MASATOSHI; MASHIMO YUKO; HIRST JUDY; MIYOSHI HIDETO
日本生体エネルギー研究会討論会講演要旨集, 2011

ミトコンドリア複合体‐Iに作用する光反応性フェンピロキシメートの結合部位の同定
SHIRAISHI YUSUKE; MURAI MASATOSHI; SAKIYAMA NAOTO; MIYOSHI HIDETO
日本生体エネルギー研究会討論会講演要旨集, 2011

酵母Type II NADH脱水素酵素(NDH‐2)において活性酸素の生成が負に制御される分子機構の解析
YAMASHITA TETSUO; YANG YU; OGISO(NAKAMARU) EIKO; HASHIMOTO TAKESHI; MURAI MASATOSHI; MIYOSHI HIDETO; IGARASHI JUNSUKE; YAGI(MATSUNO) AKEMI; YAGI TAKAO; KOSAKA HIROAKI
日本生体エネルギー研究会討論会講演要旨集, 2011

酵母シングルサブユニット・NADH‐キノン酸化還元酵素(Ndi1)の反応機構:活性酸素の生成抑制機構の解析
YAMASHITA TETSUO; YANG YU; NAKAMARU EIKO; HASHIMOTO TAKESHI; MURAI MASATOSHI; IGARASHI JUNSUKE; MIYOSHI HIDETO; YAGI AKEMI; YAGI TAKAO; KOSAKA HIROAKI
生化学, 2011

呼吸鎖酵素最後の秘境複合体‐I(NADH‐ユビキノン酸化還元酵素)の構造と機能
MURAI MASATOSHI; MIYOSHI HIDETO
化学, 01 Jan. 2011

光反応性アセトゲニンの合成と作用機構研究
NAKANISHI SAYO; YAMAMOTO SHUHEI; ABE MASATO; MURAI MASATOSHI; MIYOSHI HIDETO
日本農芸化学会関西支部講演会講演要旨集, 02 Oct. 2010

Characterization of the binding site of Delta lac-acetogenin in bovine mitochondrial complex I by photoaffinity labeling
Masatoshi Murai; Nobuyuki Kakutani; Naoto Sakiyama; Hideto Miyoshi
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Jul. 2010

ミトコンドリア複合体‐I阻害剤Δlac‐アセトゲニンの結合部位の同定
MURAI MASATOSHI; KAKUTANI NOBUYUKI; SAKIYAMA NAOTO; MIYOSHI HIDETO
日本農薬学会大会講演要旨集, 21 Apr. 2010

光反応性アセトゲニンの合成と作用機構研究
ABE MASATO; YAMAMOTO SHUHEI; MURAI MASATOSHI; MIYOSHI HIDETO
日本農薬学会大会講演要旨集, 21 Apr. 2010

広汎なカルジオリピン類の有機合成と生理活性評価
ABE MASATO; OYAMA SHUNJI; KOBORI SHIN'YA; MORIYAMA IKUKO; MURAI MASATOSHI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集, 05 Mar. 2010

ミトコンドリア複合体‐I阻害剤Δlac‐アセトゲニンの結合部位の同定
MURAI MASATOSHI; SUMIYA SHINJI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集, 05 Mar. 2010

ミトコンドリア複合体‐I阻害剤Δlac‐アセトゲニンの結合部位の同定
SUMIYA SHINJI; MURAI MASATOSHI; MIYOSHI HIDETO
日本農芸化学会関西支部講演会講演要旨集, 2010

酵母の非プロトン輸送性NADH‐ユビキノン酸化還元酵素(Ndi1)のユビキノン結合部位の解明
MASHIMO YUKO; MURAI MASATOSHI; YAMASHITA TETSUO; MIYOSHI HIDETO
日本生体エネルギー研究会討論会講演要旨集, 2010

ミトコンドリア複合体‐I阻害剤Δlac‐アセトゲニンの結合部位の解明
MURAI MASATOSHI; KAKUTANI NOBUYUKI; SAKIYAMA NAOTO; ABE MASATO; MIYOSHI HIDETO
日本生体エネルギー研究会討論会講演要旨集, 2010

ミトコンドリア複合体‐Iに作用する光反応性アセトゲニンの合成と結合部位の解析
MURAI MASATOSHI; SEKIGUCHI KOJI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集, 05 Mar. 2009

光親和性標識法によるミトコンドリア複合体‐I阻害剤キナゾリンの結合部位の解析
SEKIGUCHI KOJI; MURAI MASATOSHI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集, 05 Mar. 2009

光親和性標識法によるミトコンドリア複合体‐I阻害剤キナゾリンの結合部位の解析
SEKIGUCHI KOJI; MURAI MASATOSHI; MIYOSHI HIDETO
日本農薬学会大会講演要旨集, 27 Feb. 2009

ミトコンドリア複合体‐Iに作用するアセトゲニンの結合部位の解析
MURAI MASATOSHI; SEKIGUCHI KOJI; MIYOSHI HIDETO
日本農薬学会大会講演要旨集, 27 Feb. 2009

Identification of the inhibitor binding SITE(S) in bovine complex-i by photoaffinity labeling
Masatoshi Murai; Naoya Ichimaru; Takao Yagi; Hideto Miyoshi
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Jul. 2008

ミトコンドリア複合体‐I阻害剤の結合部位解明―光親和性標識実験によるアプローチ―
MURAI MASATOSHI; NISHIOKA TAKAAKI; MIYOSHI HIDETO
日本農薬学会大会講演要旨集, 11 Mar. 2008

ミトコンドリア複合体‐Iの新規阻害剤Δlac‐アセトゲニンの合成と作用機構研究
ICHIMARU NAOYA; MURAI MASATOSHI; NISHIOKA TAKAAKI; MIYOSHI HIDETO
日本農薬学会大会講演要旨集, 11 Mar. 2008

光親和性標識法を用いたミトコンドリア複合体‐I阻害剤の結合部位の解析
MURAI MASATOSHI; NISHIOKA TAKAAKI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集, 05 Mar. 2008

光親和性標識法によるΔlac‐アセトゲニンの結合部位同定
MURAI MASATOSHI; ICHIMARU NAOYA; NISHIOKA TAKAAKI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集, 05 Mar. 2007

ミトコンドリア複合体‐Iの新規阻害剤Δlac‐アセトゲニンの作用機構
ICHIMARU NAOYA; MURAI MASATOSHI; NISHIOKA TAKAAKI; MIYOSHI HIDETO
日本生体エネルギー研究会討論会講演要旨集, 2007

ミトコンドリア複合体‐Iにおける阻害剤結合部位の解明
MURAI MASATOSHI; ICHIMARU NAOYA; NISHIOKA TAKAAKI; MIYOSHI HIDETO
日本生体エネルギー研究会討論会講演要旨集, 2007

Δ‐lacアセトゲニンの複合体‐Iに対する作用機構研究
MURAI MASATOSHI; ICHIMARU NAOYA; ABE MASAHITO; NISHIOKA TAKAAKI; MIYOSHI HIDEHITO
日本農芸化学会大会講演要旨集, 05 Mar. 2006

新規アセトゲニンmimics(Δlac‐アセトゲニン)の合成と作用機構研究
ICHIMARU NAOYA; MURAI MASATOSHI; ABE MASAHITO; SENO MAI; MAKABE HIDEFUMI; NISHIOKA TAKAAKI; MIYOSHI HIDEHITO
日本農芸化学会大会講演要旨集, 05 Mar. 2006

ミトコンドリア複合体‐I阻害剤アセトゲニン類の阻害機構研究:スペーサー部の機能
ABE MASAHITO; MURAI MASATOSHI; ICHIMARU NAOYA; YOSHIDA TAKEHIKO; KUBO AKINA; NISHIOKA TAKAAKI; MIYOSHI HIDEHITO
日本農芸化学会大会講演要旨集, 05 Mar. 2006

ミトコンドリア複合体‐Iの新規阻害剤Δlac‐アセトゲニンの開発
MURAI MASATOSHI; ICHIMARU NAOYA; NISHIOKA TAKAAKI; MIYOSHI HIDETO
日本農芸化学会関西支部講演会講演要旨集, 2006

バンレイシ科アセトゲニンrollicosin,murisolinの合成研究
HATTORI YASUNAO; KIMURA YUKA; MORODA AKI; HIGUCHI MASAHARU; KONNO HIROYUKI; MURAI MASATOSHI; MIYOSHI HIDETO; MAKABE HIDEFUMI
天然有機化合物討論会講演要旨集, 15 Sep. 2005

Δlac‐アセトゲニン類の合成と作用機構研究
ICHIMARU NAOYA; MURAI MASATOSHI; ABE MASATO; HAMADA TSUYOSHI; MAKINO SAE; MAKABE HIDEFUMI; NISHIOKA TAKAAKI; MIYOSHI HIDETO
日本農芸化学会大会講演要旨集, 05 Mar. 2005

ミトコンドリア複合体‐I阻害剤アセトゲニンの阻害機構研究:スペーサー部の機能
ABE MASATO; MURAI MASATOSHI; ICHIMARU NAOYA; YOSHIDA TAKEHIKO; NISHIOKA TAKAAKI; MIYOSHI HIDEHITO
日本農芸化学会関西支部講演会講演要旨集, 2005

新規アセトゲニンmimics(Δlac‐アセトゲニン)の合成と作用機構研究
ICHIMARU NAOYA; MURAI MASATOSHI; ABE MASATO; HAMADA TAKESHI; MAKINO SAE; MAKABE HIDEFUMI; NISHIOKA TAKAAKI; MIYOSHI HIDEHITO
日本農芸化学会関西支部講演会講演要旨集, 2005

Presentations

Elucidation of;he;mechanism for inhibitor;resistance in the Na+-translocating NADH-ubiquinone;oxidoreductase from Vibrio cholerae
Takahiro Masuya; Moe Ishikawa; Jun-ichi Kishikawa; Masatoshi Murai; Danielle McFee; Takayuki Kato; Blanca Barquera; Hideto Miyoshi
Pesticide Science Society of Japan, 50th annual meeting, 13 Mar. 2025

Identification of the binding site of mycothiazole in respiratory complex I
Ryohei Otani; Shuji Miyako; Hiroki Saito; Takahiro Masuya; Masatoshi Murai; Hideto Miyoshi
Pesticide Society of Japan 50th annual meeting, 13 Mar. 2025

合成ユビキノン類を用いた出芽酵母におけるユビキノン取り込みの分子機構研究
桜井直也; 水谷みらい; 奥公秀; 三芳秀人; 村井正俊
日本農芸化学会関西支部第534回講演会, 08 Feb. 2025

The reaction mechanism of decoupling ubiquinone with respiratory complex I
Kohei TOMIZAWA; Tomoki KOSHITAKA; Takahiro MASUYA; Masatoshi MURAI; Hideto MIYOSHI
JSBBA 2025 Annual Meeting, 08 Mar. 2025

Comprehensive screening of proteins involved in intracellular ubiquinone trafficking in Saccharomyces cerevisiae using artificial ubiquinone probe
Masatoshi MURAI Mirai MIZUTANI Seina; KURODA Masahide OKU Wataru AOKI Takahiro MASUYA; Hideto MIYOSHI
JSBBA 2025 Annual Meeting, 06 Mar. 2025

Pinpoint introduction of functional molecular probe into the NqrB subunit of Na+-translocating NADH-ubiquinone oxidoreductase from Vibrio cholerae
Saya Miyachi Hinako; Tanaka Moe; Ishikawa Blanca; Barquera Takahiro Masuya; Masatoshi Murai; Hideto Miyoshi
JSBBA 2025 Annual Meeting, 06 Mar. 2025

Identification of the binding site of mycothiazole in respiratory complex I
Ryohei Otani; Shuji Miyako; Hiroki Saito; Takahiro Masuya; Masatoshi Murai; Hideto Miyoshi
JSBBA 2025 Annual Meeting, 06 Mar. 2025

合成ユビキノンプローブを用いた出芽酵母のユビキノン取り込みに関与するタンパク質の探索
村井正俊
2024年度京都大学・生理化学研究ユニットシンポジウム, 20 Dec. 2024, Invited

Comprehensive screening of proteins involved in intracellular ubiquinone trafficking in Saccharomyces cerevisiae using artificial ubiquinone probe
Murai, M.; Mizutani, M.; Kuroda, S.; Oku, M.; Aoki, W.; Masuya, T.; Miyoshi, H.
Japan Bioenergetics Group 50th meeting, 14 Dec. 2024

「化学」と「生物」で解き明かすエネルギー代謝酵素の構造と機能
村井正俊
第18回関西創農薬研究会, 11 Oct. 2024, Invited

Identification of proteins involved in intracellular ubiquinone trafficking in Saccharomyces cerevisiae using artificial ubiquinone probe,
Murai, M.; Mizutani, M.; Kuroda, S.; Oku, M.; Aoki, W.; Masuya, T.; Miyoshi, H.
The 22th European Bioenergetics Conference, 28 Aug. 2024

「化学」と「生物」で解き明かすエネルギー代謝酵素の構造と機能
村井正俊
京都大学若手研究者産学連携ネットワークイベント：Transform桂, 22 Feb. 2024, Invited

Elcidating the action mechanism of inhibitors against Na+-pumping NADH-quinone oxidoreductase from Vibrio cholera
Moe Ishikawa; Jun-ichi Kishikawa; Takahiro Masuya; Masatoshi Murai; Takayuki Kato; Blanca Barquera; Hideto Miyoshi
Japan Society of Bioscience, Biotechnology, and Agrochemistry, 2024 annual meeting, 27 Mar. 2024

Comprehensive screening of the proteins involved in the trafficking of coenzyme Q in Saccharomyces cerevisiae using a synthetic ubiquinone probe
Mirai Mizutani; Seina Kuroda; Masahide Oku; Takahiro Masuya; Masatoshi Murai; Hideto Miyoshi
Japan Society of Bioscience, Biotechnology, and Agrochemistry, 2024 annual meeting, 26 Mar. 2024

Defining the action mechanism of diariyisooxazole-3-caboxamides, small molecule inhibitors targeting the mitochondrial permeability transition
Taiga Watanabe; Takahiro Masuya; Masatoshi Murai; Hideto Miyoshi
Japan Society of Bioscience, Biotechnology, and Agrochemistry, 2024 annual meeting, 26 Mar. 2024

Characterization of the quinone-binding pocket of respiratory complex I using a PC-quinazoline hybrid inhibitor
Ryohei Otani; Yusuke Shimura; Takahiro Masuya; Masatoshi Murai; Hideto Miyoshi
Japan Society of Bioscience, Biotechnology, and Agrochemistry, 2024 annual meeting, 26 Mar. 2024

Characterization of the conformational change of Na+-pumping NADH-quinone oxidoreductase from Vibrio cholerae through specific chemical modification
Saya Miyachi; Hinako Tanaka; Moe Ishikawa; Blanca Barquera; Takahiro Masuya; Masatoshi Murai; Hideto Miyoshi
Japan Society of Bioscience, Biotechnology, and Agrochemistry, 2024 annual meeting, 25 Mar. 2024

Defining the action mechanism of diarylisoxazole-3-carboxamides (DICs), small molecule inhibitors targeting the mitochondrial permeability transition
Taiga Watanabe; Takahiro Masuya; Hideto Miyoshi; Masatoshi Murai
Pesticide Science Society of Japan, 49th annual meeting, 15 Mar. 2024

Characterization of the quinone reaction site of respiratory complex I using synthetic quinone probes
Takahiro Masuya; Ryo Ikunishi; Ryohei Otani; Masatoshi Murai; Tomoo Shiba; Hideto Miyoshi
Japan Bioenergetics Group 49th annual meeting, 16 Dec. 2023

Elucidating the action mechanism of inhibitors against Na+-pumping NADH-quinone oxidoreductase from Vibrio cholerae
Moe Ishikawa; Jun-ichi Kishikawa; Takahiro Masuya; Masatoshi Murai; Takayuki Kato; Blanca Barquera; Hideto Miyoshi
Japan Bioenergetics Group 49th annual meeting, 15 Dec. 2023

Mode of action study of rotenone in mitochondrial respiratory complex I
Takahiro Masuya; Masatoshi Murai; Hideto Miyoshi
Pesticide Science Society of Japan, 49th annual meeting, 16 Mar. 2023

The reaction mechanism of decoupling quinone with mitochondrial respiratory complex I
Tomoki Koshitaka; Kohei Tomizawa; Takahiro Masuya; Masatoshi Murai; Hideto Miyoshi
JSBBA 2023 Annual Meeting, 15 Mar. 2023

Characterization of the quinone binding pocket of respiratory complex I using synthetic quinone probes
Ryo Ikunishi; Ryohei Otani; Takahiro Masuya; Masatoshi Murai; Tomoo Shiba; Hideto Miyoshi
JSBBA 2023 Annual Meeting, 15 Mar. 2023

Identification of the binding site of acetogenin in mitochondrial respiratory complex I
Misaki Nishida; Takahiro Masuya; Masatoshi Murai; Hideto Miyoshi
JSBBA 2023 Annual Meeting, 15 Mar. 2023

Cryo-EM structures of Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae
Moe Ishikawa; Jun-ichi Kishikawa; Takahiro Masuya; Masatoshi Murai; Yuki Kitazumi; Nicole Butler; Takayuki Kato; Hideto Miyoshi
JSBBA 2023 Annual Meeting, 15 Mar. 2023

Investigation of the binding site of UQ side chain in Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae
Moe Ishikawa; Takahiro Masuya; Masatoshi Murai; Nicole Butler; Blanca Barquera; Hideto Miyoshi
Pesticide Science Society of Japan 48th Meeting, 09 Mar. 2023

Identification of the binding site of acetogenin in mitochondrial respiratory complex I
Misaki Nishida; Takahiro Masuya; Masatoshi Murai; Hideto Miyoshi
Pesticide Science Society of Japan 48th meeting, 09 Mar. 2023

Exploring the ubiquinone-reaction site in mitochondrial complex I using synthetic quinones
Takahiro Masuya; Shinpei Uno; Tomoki Koshitaka; Ryo Ikunishi; Kohei Tomizawa, Naoya; Sakurai; Kenji Okuda; Masatoshi Murai; Hideto Miyoshi
The Japan Bioenergetics Group 48th Meeting, 16 Dec. 2022

Investigation of the binding site of UQ side chain in Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae
Moe Ishikawa; Takahiro Masuya; Masatoshi Murai; Nicole L. Butler; Blanca Barquera; Hideto Miyoshi
The Japan Bioenergetics Group 48th Meeting, 14 Dec. 2022

Cryo-EM structures of Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae
Moe Ishikawa; Jun-ichi Kishikawa; Takahiro Masuya; Masatoshi Murai; Yuki Kitazumi; Nicole L. Butler; Takayuki Kato; Blanca Barquera; Hideto Miyoshi
The 60th Annual Meeting of the Biophysical Society of Japan, 29 Sep. 2022

The side chain regulates diverse reaction manners of artificial ubiquinones in mitochondrial respiratory complex I
Shinpei Uno; Ryo Ikunishi; Takahiro Masuya; Kyoko Shinzawa-Itoh; Masatoshi Murai; Hideto Miyoshi
Japan Bioenergetics Group 47th meetinh, 17 Dec. 2021

Comprehensive understanding of multiple actions of anticancer drug tamoxifen in isolated mitochondria
Yufu Unten; Masatoshi Murai; Tomoki Koshitaka; Kotaro Kitao; Osamu Shirai; Takahiro Masuya; Hideto Miyoshi
Japan Bioenergetics Group 47th meeting, 17 Dec. 2021

The side chain of ubiquinone plays a critical role in Na+ translocation by NADH-ubiquinone oxidoreductase from Vibrio cholerae
Ishikawa M; Masuya T; Kuroda S; Uno S; Shiba T; Inaoka DK; Murai M; Barquera B; Miyoshi H
Japan Bioenergetics Group 47th meeting, 17 Dec. 2021

Inhibition mechanism of manassantin as an inhibitor of mitochondrial respiratory complex I
Nagaoka C; Murai M; Masuya T; Miyoshi H
The 2021 annual meeting of JSBBA, 20 Mar. 2021

Mode of action study of fusaramin, a natural tetramic acid derivative targeting Saccharomyces cerevisiae mitochondria
Unten Y; Murai M; Sakai K; Asami Y; Masuya T; Miyoshi H
The 2021 annal meeting of JSBBA, 20 Mar. 2021

IACS-010759, a potent inhibitor of glycolysis-deficient hypoxic tumor cells, inhibits mitochondrial respiratory complex I through a unique mechanism
Murai M; Tsuji A; Akao T; Masuya T; Miyoshi H
The 2021 annual meeting of JSBBA, 20 Mar. 2021

Pinpoint chemical labeling of the NqrB subunit in Vibrio cholerae Na+-pumping NADH-quinone oxidoreductase
Ishikawa M; Tanaka H; Masuya T; Barquera B; Murai M; Miyoshi H
The 2021 annal meeting of JSBBA, 20 Mar. 2021

Site-specific chemical cross-linking for elucidating the ubiquinone reaction site in mitochondrial complex I
Masuya T; Uno S; Murai M; Miyoshi H
The 2021 annual meeting of JSBBA, 20 Mar. 2021

Exploring the quinone-binding pocket in mitochondrial respiratory complex I using synthetic quinones as molecular probes
Uno S; Masuya T; Shinzawa-Ito K; Shiba T; Inaoka DK; Murai M; Miyoshi H
The 2021 Annual Meeting of JSBBA, 20 Mar. 2021

Exploring the quinone-binding pocket in mitochondrial respiratory complex I using synthetic quinones as molecular probes
Uno S; Masuya T; Shinzawa-Ito K; Shiba T; Inaoka DK; Murai M; Miyoshi H
Pesticide Science Society of Japan, 46th annual meeting, 10 Mar. 2021

Specific chemical modification of the NqrB subunit in Na+-pumping NADH-quinone oxidoreductase from Vibrio cholerae
Ishikawa M; Masuya T; Tanaka H; Murai M; Barquera B; Miyoshi H
Pesticide Science Society of Japan, 46th annual meeting, 09 Mar. 2021

Site-specific chemical cross-linking for elucidating the ubiquinone reaction site in mitochondrial complex I
Masuya T; Uno S; Murai M; Miyoshi H
Japan Bioenergetics Group 46th meeting, 11 Dec. 2020

Inhibition mechanism of mitochondrial respiratory complex I by IACS-010759, a candidate compound of anticancer agent
Tsuji A; Akao T; Masuya T; Murai M; Miyoshi H
JSBBA, 2020 annual meeting, 28 Mar. 2020

Site-specific chemical modification of Na+-pumping NADH-quinone oxidoreductase from Vibrio cholerae
石川萌; 桝谷貴洋; 村井正俊; Blanca Barquera; 三芳秀人
JSBBA, 2020 annual meeting, 28 Mar. 2020

Identification of the inhibitor-binding site in Na+-pumping NADH-quinone oxidoreductase from Vibrio cholerae
Sano Y; Masuya T; Murai M; Barquera B; Miyoshi H
JSBBA, 2020 annual meeting, 28 Mar. 2020

Exploring the quinone-binding pocket in mitochondrial respiratory complex I using synthetic quinones as molecular probes
Uno S; Masuya T; Shinzawa-Ito K; Shiba T; Inaoka DK; Murai M; Miyoshi H
JSBBA, 2020 annual meeting, 26 Mar. 2020

Exploring the quinone-binding pocket in mitochondrial respiratory complex I using synthetic quinones as molecular probes
Uno S; Masuya T; Shinzawa-Ito K; Shiba T; Inaoka DK; Murai M; Miyoshi H
日本農薬学会第45回大会（コロナウイルス感染拡大により中止）, 09 Mar. 2020

Inhibition mechanism of mitochondrial respiratory complex I by IACS-010759, a potent inhibitor of glycolysis-deficient hypoxic tumor cells
Tsuji A; Akao T; Masuya T; Murai M; Miyoshi H
Pesticide Science Society of Japan, 45th annual meeting, 09 Mar. 2020

Identification of the korormicin-binding site in Na+-pumping NADH-quinone oxidoreductase from Vibrio cholerae
Masuya T; Sano Y; Tanaka H; Ito T; Barquera B; Murai M; Miyoshi H
Pesticide Science Society of Japan, 45th annual meeting, 09 Mar. 2020

Defining the mechanism of action of S1QELs, specific suppressors of superoxide production in the quinone-binding site in mitochondrial complex I
Murai M; Banba A; Tsuji A; Miyoshi H
Japan Bioenergetics Group 45th meeting, 22 Dec. 2019

Mode of action study of pentanediol-type compounds, specific inhibitors of VDAC1 in Saccharomyces cerevisiae mitochondria
Unten Y; Murai M; Yamamoto T; Shinohara Y; Ichimaru N; Miyoshi H
Japan Bioenergetics Group 45th meeting, 21 Dec. 2019

Oversized ubiquinone as a molecular probe for structural dynamics of the ubiquinone reaction site in mitochondrial respiratory complex I
Uno S; Masuya T; Shinzawa-Ito K; Shiba T; Inaoka D. K; Murai M; Miyoshi H
Japan Bioenergetics Group 45th meeting, 21 Dec. 2019

Mode of action study of pentanediol-type compounds, specific inhibitors of VDAC1 in Saccharomyces cerevisiae mitochondria
Unten Y; Murai M; Yamatomo T; Ichimaru N; Shinohara Y; Miyoshi H
JSBBA Kansai-Chubu 2019 annual meeting, 22 Sep. 2019

Exploring the structure and function of mitochondrial respiratory complex I through chemical biology approaches
Masatoshi Murai
JSBBA-Kansai 508th meeting, 01 Jun. 2019, Invited

Exploring the quinone/inhibitor-binding pocket in mitochondrial respiratory complex I using synthetic quinones and inhibitors
Uno S; Kimura H; Murai M; Miyoshi H
JSBBA-Kansai 508th meeting, 01 Jun. 2019

Action manner of S1QELs, specific suppressors of superoxide production from mitochondrial respiratory complex I
Banba A; Tsuji A; Murai M; Miyoshi H
JSBBA, the 2019 annual meeting, 26 Mar. 2019

Identification of the binding site of amilorides in respiratory complex I
Kimura H; Murai M; Miyoshi H
JSBBA, the 2019 annual meeting, 26 Mar. 2019

Pentenediol-type compounds specifically inhibit mitochondrial VDAC1 in Saccharomyces cerevisiae mitochondria
Unten Y; Murai M; Ichimaru N; Yamamoto T; Shinohara Y; Miyoshi H
JSBBA, the 2019 annual meeting, 26 Mar. 2019

Exploring the quinone binding pocket in respiratory complex I using synthetic quinone analogues
Uno S; Murai M; Miyoshi H
JSBBA, the 2019 annual meeting, 26 Mar. 2019

Mechanism of aggregation of mitochondrial ADP/ATP carrier induced by epoxycyclohexenedione-type compounds
Aoyama A; Murai M; Miyoshi H
JSBBA, the 2019 annual meeting, 26 Mar. 2019

Action manner of S1QELs, specific suppressors of superoxide production from mitochondrial respiratory complex I
Banba A; Tsuji A; Kimura H; Murai M; Miyoshi H
Pesticide Science Society of Japan, 44th annual meeting, 12 Mar. 2019

Exploring the quinone/inhibitor-binding pocket in mitochondrial respiratory complex I
Uno S; Kimura H; Murai M; Miyoshi H
Pesticide Society of Japan, 44th meeting, 12 Mar. 2019

ミトコンドリア呼吸鎖複合体-Iの機能解明を目指した生物有機化学的研究：低分子化合物で紐解く巨大タンパク質の構造と機能
Masatoshi Murai
有機化学研究会（白鷺セミナー）第84回講演会, 11 Dec. 2018

Exploring the quinone/inhibitor-binding pocket in complex I by chemical biology approaches
Murai M; Uno S; Kimura H; Miyoshi H
Japan Bioenergetics Group 44th meeting, 08 Dec. 2018

Characterization of the quinone-binding pocket of mitochondrial respiratory complex I through specific chemical modifications
Murai M; Masuya T; Miyoshi H
19th European Bioenergetics Conference (EBEC2018), 25 Aug. 2018

Epoxycyclohexenedione-type compounds make up a new class of inhibitors of the bovine mitochondrial ADP/ATP carrierEpoxycyclohexenedione-type Compounds Make Up a New Class of Inhibitors of the Bovine Mitochondrial ADP/ATP Carrier
Aoyama A; Murai M; Miyoshi, H
19th European Bioenergetics Conference (EBEC2018), 25 Aug. 2018

Epoxycyclohexenedione-Type Compounds Make Up a New Class of Inhibitors of the Bovine Mitochondrial ADP/ATP Carrier
Aoyama A; Murai M; Ichimaru N; Miyoshi H
Pesticide Science Society of Japan, 43th annual meeting, 27 May 2018

Characterization of quinone-binding pocket of respiratory complex I through chemical modifications
Murai M; Masuya T; Miyoshi H
Pesticide Science Society of Japan, 43th annual meeting, 27 May 2018

Mode of action study of pentenediol derivatives against VDAC in Saccharomyces cerevisiae mitochondria
Unten Y; Murai M; Ichimaru N; Yamamoto T; Shinohara Y; Miyoshi H
JSBBA Annual Meeting 2018, 17 Mar. 2018

Identification of the binding site of amilorides in respiratory complex I
Kimura H; Murai M; Miyoshi H
JSBBA Annual Meeting 2018, 17 Mar. 2018

Mechanism of the inhibition of mitochondrial ADP/ATP carrier by epoxycyclohexenedione-type compounds
Aoyama A; Murai M; Ichimaru N; Miyoshi H
JSBBA Annual Meeting 2018, 17 Mar. 2018

Identification of the inhibitor binding site in the Na+-pumping NADH-quinone oxidoreductase from Vibrio cholerae
Ito T; Murai M; Ninokura S; Kitazumi Y; Barquera B; Miyoshi H
JSBBA Annual Meeting 2018, 17 Mar. 2018

Pinpoint chemical modification of the quinone-access channel of respiratory complex I
Murai M; Masuya T; Miyoshi H
Japan Bioenergetics Group 43th meeting, 21 Dec. 2017

Identification of the inhibitor binding site in the Na+-pumping NADH-quinone oxidoreductase from Vibrio cholerae
Ito T; Murai M; Ninokura S; Kitazumi Y; Barquera B; Miyoshi H
Japan Bioenergetics Group 43th meeting, 21 Dec. 2017

Pinpoint chemical modification of the quinone-access channel of mitochondrial complex I via a two-step conjugation reaction
Murai M; Masuya T; Miyoshi H
JSBBA-Kansai-Annual Meeting, 22 Sep. 2017

Mechanism of the inhibition of bovine heart mitochondrial ADP/ATP carrier by epoxycyclohexenedione-type inhibitors
Aoyama A; Murai M; Miyoshi H
JSBBA-Kansai-Annual Meeting, 22 Sep. 2017

Identification of the inhibitor binding site in Na+-pumping NADH-quinone oxidoreductase from Vibrio cholerae
Ito T; Murai M; Barquera B; Miyoshi H
JSBBA-Kansai-annunal meeting, 22 Sep. 2017

Characterization of the ubiquinone-binding pocket of respiratory complex I through chemical modifications
Masatoshi Murai
The 2017 Gordon Research Conference on Bioenergetics, 07 Jun. 2017, Invited

Synthetic ubiquinones specifically bind to mitochondrial voltage-dependent anion channel (VDAC) in Saccharomyces cerevisiae mitochondria
Murai M; Okuda A; Yamamoto T; Shinohara Y; Miyoshi H
JSBBA annual meeting 2017, 20 Mar. 2017

Isolation and total synthesis of korormicin, a potent inhibitor of sodium-translocating NADH-ubiquinone oxidoreductase from Vibrio cholerae
Ito T; Tosaki T; Murai M; Barquera B; Miyoshi H
JSBBA annual meeting 2017, 20 Mar. 2017

Site-specific chemical modification of mitochondrial respiratory complex I
Masuya T; Murai M; Miyoshi H
JSBBA annual meeting 2017, 20 Mar. 2017

Specific methylation of the ubiquinone binding cavity of bovine mitochondrial complex I via ligand-directed tosyl chemistry
Masuya T; Inaoka H; Murai M; Miyoshi H
Pesticide Science Society of Japan, 42th meeting, 08 Mar. 2017

Synthetic ubiquinones specifically bind to mitochondrial voltage-dependent anion channel1 (VDAC) in Saccharomyces cerevisiae
Murai M; Okuda A; Yamamoto T; Shinohara Y; Miyoshi H
Pesticide Science Society of Japan, 42th meeting, 08 Mar. 2017

Isolation and total synthesis of korormicin, an inhibitor of sodium-translocating NADH-ubiquinone oxidoreductase from Vibrio cholerae
Ito T; Tosaki T; Murai M; Barquera B; Miyoshi H
Pesticide Science Society of Japan, 42th meeting, 08 Mar. 2017

Reduction of synthetic ubiquinone QT catalyzed by mitochondrial respiratory complex I is decoupled from proton translocation
Murai M; Okuda K; Masuya T; Miyoshi H
Japan Bioenergetics Group 42th meeting, 19 Dec. 2016

Site-specific chemical modification of mitochondrial respiratory complex I
Masuya T; Murai M; Inaoka H; Miyoshi H
Japan Bioenergetics Group 42th meeting, 19 Dec. 2016

Identification of the binding site of amilorides in mitochondrial respiratory complex I
Ito T; Murai M; Miyoshi H
18th European Bioenergetics Conference (EBEC2016), 02 Jul. 2016

Synthetic ubiquinones specifically bind to mitochondrial voltage-dependent anion channel of Saccharomyces cerevisiae
Murai M; Okuda A; Yamamoto T; Shinohara Y; Miyoshi H
18th European Bioenergetics Conference (EBEC2016), 02 Jul. 2016

Site-specific chemical modifications of the quinone binding cavity of bovine heart mitochondrial complex I
TMasuya T; Murai M; Inaoka H; Miyoshi H
18th European Bioenergetics Conference (EBEC2016), 02 Jul. 2016

Reduction of synthetic ubiquinone QT catalyzed by bovine mitochondrial complex I is decoupled with proton translocation
Okuda K; Murai M; Aburaya S; Aoki W; Miyoshi H
JSBBA annual meeting 2016, 29 Mar. 2016

Specific methylation of ubiquinone-binding pocket in mitochondrial complex I through Tosyl chemistry
Inaoka H; Murai M; Masuya T; Aburaya S; Aoki W; Miyoshi H
JSBBA annual meeting 2016, 29 Mar. 2016

Mitochondrial voltage dependent anion channel of Saccharomyces cerevisiae specifically accommodates ubiquinone
Okuda A; Murai M; Yamamoto T; Shinohara Y; Miyoshi H
JSBBA annual meeting 2016, 29 Mar. 2016

Reduction of synthetic ubiquinone QT catalyzed by bovine mitochondrial complex I is decoupled from proton translocation
Masuya T; Okuda K; Murai M; Miyoshi H
Pesticide Science Society of Japan, 41th meeting, 19 Mar. 2016

Characterization of amilorides as potent inhibitors of mitochondrial respiratory complex I
Ito T; Murai M; Murakami S; Morisaka H; Miyoshi H
Pesticide Science Society of Japan, 41th meeting, 19 Mar. 2016

Action mechanism of mitochondrial respiratory complex I inhibitors
Miyoshi H; Murai M
Pesticide Science Society of Japan, 41th meeting (Symposium), 18 Mar. 2016, Invited

Characterization of the ubiquinone-binding protein Coq10 using a synthetic ubiquinone probe
Murai M; Miyoshi H
Pesticide Science Society of Japan 41th meeting (Symposium), 18 Mar. 2016, Invited

Pinpoint chemical modification of mitochondrial NADH-ubiquinone oxidoreductase (complex I)
Masuya T; Murai M; Morisaka H; Miyoshi H
30th Meeting of committee on pesticide design, 12 Nov. 2015

Identification of the binding site of amilorides in mitochondrial NADH-ubiquinone oxidoreductase (complex I)
Ito T; Murai M; Murakami M; Morisaka H; Miyoshi H
30th Meeting of commettee on pesticide design, 12 Nov. 2015

Amilorides bind to the quinone binding pocket of bovine mitochondrial complex I
Murai M; Miyoshi H
Gordon Research Conference on Bioenergetics, 21 Jun. 2015

Pinpoint chemical modification of mitochondrial NADH-ubiquinone oxidoreductase (complex I)
Masuya T; Murai M; Morisaka H; Miyoshi H
JSBBA annual meeting 2015, 27 Mar. 2015

Identification of the binding site of amilorides in mitochondrial complex I
Ito T; Murai M; Morisaka H; Miyoshi H
JSBBA annual meeting 2015, 27 Mar. 2015

Identification of the ubiquinone-binding site in Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae
Ninokura S; Murai M; Barquera B; Miyoshi H
JSBBA annual meeting 2015, 27 Mar. 2015

Characterization of the ubiquinone-binding site in yeast mitochondrial voltage dependent anion channnel
Okuda A; Murai M; Miyoshi H
JABBA annual meeting 2015, 27 Mar. 2015

Pinpoint chemical modification of mitochondrial complex I
Takahiro Masuya; Masatoshi Murai; Hironobu Morisaka; Hideto Miyoshi
Pesticide Science Society of Japan, 40th meeting, 20 Mar. 2015

Synthesis and characterization of amilorides as potent inhibitors of mitochondrial respiratory complex I
Masatoshi Murai; Sonomi Murakami; Takeshi Ito; Hideto Miyoshi
Pesticide Science Society of Japan 40th meeting, 20 Mar. 2015

Pinpoint chemical modification of mitochondrial NADH-ubiquinone oxidoreductase (complex I)
Takahiro Masuya; Masatoshi Murai; Hironobu Morisaka; Hideto Miyoshi
40th Japan Bioenergetics Group Meeting (JBEG2014), 13 Dec. 2014

Site-Specific Chemical Modification of Mitochondrial NADH-Ubiquinone Oxidoreductase (Complex I) Through Ligand-Directed Tosylate Chemistry
Murai M; Masuya T; Morisaka H; Ifuku K; Miyoshi H
18th European Bioenergetics Conference (EBEC2014), 12 Jul. 2014

Identification of the binding site of quinazoline in mitochondrial ADP/ATP carrier
Shu Asano; Masatoshi Murai; Hideto Miyoshi
JSBBA annual meeting 2014, 29 Mar. 2014

Site-specific chemical labeling of mitochondrial NADH-ubiquinone oxidoreductase (complex I)
Takahiro Masuya; Masatoshi Murai; Hideto Miyoshi
JSBBA annual meeting 2014, 29 Mar. 2014

Identification of ubiquinone binding site in mitochondrial Coq10 using a synthetic ubiquinone probe
Kohei Matsunobu; Masatoshi Murai; Makoto Kawamukai; Hideto Miyoshi
JSBBA annual meeting 2014, 29 Mar. 2014

Synthesis and characterization of amiloride derivatives as inhibitors of mitochondrial complex I
Sonomi Murakami; Takeshi Ito; Sayako Habu; Masaoshi Murai; Hideto Miyoshi
Pesticide Science Society of Japan 39th annual meeting, 14 Mar. 2014

Site-specific chemical labeling of mitochondrial complex )
Takahiro Masuya; Masatoshi Murai; Hideto Miyoshi
Pesticide Science Society of Japan 39th annual meeting, 14 Mar. 2014

Characterization of mitochondrial Coq10 using a synthetic ubiquinone probe
Masatoshi Murai; Kohei Matsunobu; Makoto Kawamukai; Hideto Miyoshi
39th Japan Bioenergetics Group meeting (JBEG2013), 19 Dec. 2013

Characterization of mitochondrial Coq10 using a synthetic ubiquinone probe
Masatoshi Murai; Kohei Matsunobu; Sawako Kudo; Makoto Kawamukai; Hideto Miyoshi
JSBBA Kansai-Nishinihon, joint meeting, 06 Sep. 2013

Characterization of the mitochondrial ubiquinone-binding protein Coq10 using a synthetic ubiquinone probe
Masatoshi Murai; Kohei Matsunobu; Sawako Kudo; Makoto Kawamukai; Hideto Miyoshi
Gordon Research Conference on Bioenergetics, Jun. 2013

Characterization of the mitochondrial ubiquinone-‐binding protein Coq10 using a synthetic ubiquinone probe
Kohei Matsunobu; Masatoshi Murai; Sawako Kudoh; Makoto Kawamukai; Hideto Miyoshi
JSBBA Annual Meeting 2013, Mar. 2013

Synthesis and characterization of amiloride derivatives as inhibitors of mitochondrial NADH-ubiquinone oxidoreductase (complex I)
Sayako Habu; Sonomi Murakami; Masatoshi Murai; Hideto Miyoshi
JSBBA Annual Meeting 2013, Mar. 2013

Identificaon of the binding site of quinazoline-type inhibitor of mitochondrial NADH-ubiquinone oxidoreductase (complex I)
Shu Asano; Masatoshi Murai; Hideto Miyoshi
JSBBA Annual Meeting 2013, Mar. 2013

Characterization of the binding site of photolabile fenpyroximate analogues in bovine NADH-‐ubiquinone oxidoreductase (complex I)
Masatoshi Murai; Yusuke Shiraishi; Naoto Sakiyama; Hideto Miyoshi
JSBBA Annual Meeting 2013, Mar. 2013

Exploring the interaction between 49 kDa and ND1 subunits in mitochondrial complex I using a quinazoline-type inhibitor
Masatoshi Murai; Yuko Mashimo; Hideto Miyoshi
Pesticide Society of Japan, 38th Annual Meeting, Mar. 2013

Identification of the inhibitor-binding site in bovine mitochondrial NADH-ubiquinone oxidoreductase (complex I)
Masatoshi Murai; Yusuke Shiraishi; Naoto Sakiyama; Hideto Miyoshi
38th Japan Bioenergetics Group Meeting (JBEG 2012), Dec. 2012

Characterization of the inhibitor-binding site in mitochondrial NADH-Ubiquinone oxidoreductase (Complex I) using fenpyroximate analogues
Masatoshi Murai; Yusuke Shiraishi; Naoto Sakiyama; Hideto Miyoshi
17th European Bioenergetics Conference (EBEC2012), Sep. 2012

Comprehensive survey of ubiquinone-binding protein in Saccaromyces serevisiae using synthetic molecular probes
Sawako Kudo; Masatoshi Murai; Kazufumi Yazaki; Hideto Miyoshi
JSBBA Annual Meeting 2012, Mar. 2012

Identification of the inhibitor binding site in mitochondrial complex I (1): the binding site of fenpyroximate.
Yusuke Shiraishi; Masatoshi Murai; Naoto Sakiyama; Hideto Miyoshi
Pesticide Society of Japan, 37th Annual Meeting, Mar. 2012

Identification of the inhibitor binding site in mitochondrial complex I (2): an overview of the site
Masatoshi Murai; Yusuke Shiraishi; Naoto Sakiyama; Hideto Miyoshi
Pesticide Society of Japan, 37th Annual Meeting, Mar. 2012

Characterization of the binding site of photolabile fenpyroximate analogues in bovine NADH-ubiquinone oxidoreductase (complex I)
Yusuke Shiraishi; Masatoshi Murai; Naoto Sakiyama; Hideto Miyoshi
37th Japan Bioenergetics Group Meeting (JBEG 2011), Dec. 2011

Exploring interactions between the 49 kDa and ND1 subunits in mitochondrial NADH-ubiquinone oxidoreductase (complex I) using a quinazoline-type inhibitor
Masatoshi Murai; Yuko Mashimo; Hideto Miyoshi
37th Japan Bioenergetics Group Meeting (JEBG 2011), Dec. 2011

Exploring interactions between the 49 kDa and ND1 subunits in mitochondrial NADH-ubiquinone oxidoreductase (complex I) by photoaffinity labeling
Masatoshi Murai; Yuko Mashimo; Hideto Miyoshi
Nouyaku Dezain Kenkyu-kai, Nov. 2011

Synthesis of functionalized-ubiquinone probe and identification of the ubiquinone-binding protein
Sayako Habu; Masatoshi Murai; Minoru Yokoyama; Hideto Miyoshi
JSBBA-Kansai and Chubu, Joint Meeting 2011, Oct. 2011

Synthesis of photolabile ubiquinone probes and identification of the ubiquinone-binding protein
Sayako Habu; Minoru Yokoyama; Masatoshi Murai; Masato Abe; Hideto Miyoshi
JSBBA Annual Meeting 2011, Mar. 2011

Synthesis of a photolabile fenpyroximate analogue and identification of its binding site in mitochondrial complex I
Masatoshi Murai; Naoto Sakiyama; Yusuke Shiraishi; Masato Abe; Hideto Miyoshi
JSBBA Annual Meeting 2011, Mar. 2011

Identification of the binding site of photolabile fenpyroximate analogue in bovine mitochondrial complex I (II)
Naoto Sakiyama; Masatoshi Murai; Yusuke Shiraishi; Masato Abe; Hideto Miyoshi
Pesticide Society of Japan, 36th Annual Meeting, Mar. 2011

Identification of the binding site of photolabile fenpyroximate analogue in bovine mitochondrial complex I (I)
Masatoshi Murai; Naoto Sakiyama; Yusuke Shiraishi; Masato Abe; Hideto Miyoshi
Pesticide Society of Japan 38th Annual Meeting, Mar. 2011

Synthesis of functionalized-ubiqinone probes for identification of the ubiquinone-binding protein
Minoru Yokoyama; Sayako Habu; Masatoshi Murai; Masato Abe; Hideto Miyoshi
JSBBA-Kansai 473th Meeting, Jan. 2011

Characterization of the ubiquinone binding site in the alternative NADH-quinone oxidoreductase of Saccharomyces cerevisiae by photoaffinity labeling
Yuko Mashimo; Masatoshi Murai; Tetsuo Yamashita; Hideto Miyoshi
36th Japan Bioenergetics Group Meeting (JBEG 2010), Nov. 2010

Identification of the binding site of Δlac-acetogenin in bovine mitochondrial complex I
Masatoshi Murai; Nobuyuki Kakutani; Naoto Sakiyama; Masato Abe; Hideto Miyoshi
36th Japan Bioenergetics Group Meeting (JBEG 2010), Nov. 2010

Identification and Characterization of the Binding Site of Δlac-Acetogenin in Mitochondrial NADH-Ubiquinone Oxidoreductase (Complex I) by Photoaffinity Labeling
Masatoshi Murai; Nobuyuki Kakutani; Naoto Sakiyama; Hideto Miyoshi
16th European Bioenergetics Conference (EBEC2010), Jun. 2010

Identification of the binding site of Δlac-acetogenin in bovine heart mitochondrial complex I
Masatoshi Murai; Nobuyuki Kakutani; Naoto Sakiyama; Hideto Miyoshi
Pesticide Society of Japan, 35th Annual Meeting, May 2010

Identification of the Binding-Site of Δlac-Acetogenin in Bovine Mitochondrial Complex I
Masatoshi Murai; Nobuyuki Kakutani; Hideto Miyoshi
JSBBA Annual Meeting 2010, Mar. 2010

Identification of the binding site of Δlac-acetogenin in bovine heart mitochondrial NADH-ubiquinone oxidoreductase
Nobuyuki Kakutani; Masatoshi Murai; Hideto Miyoshi
JSBBA-Kansai 463th Meeting, Feb. 2010

Identification of the inhibitor binding site in mitochondrial NADH-ubiquinone oxidoreductase using a quinazoline-type inhibitor
Masatoshi Murai; Hideto Miyoshi
Nouyaku Dezain Kenkyu-kai (in Japanese), Nov. 2009

Exploring the Inhibitor Binding Site in Mitochondrial NADH-Ubiquinone Oxidoreductase (Complex I) using Photoaffinity Labeling Probes
Masatoshi Murai; Hideto Miyoshi
The 11th International Kyoto Conference on New Aspects of Organic Chemistry, Nov. 2009

Exploring the Inhibitor Binding Site in Mitochondrial Complex I by Photoaffinity Labeling
Masatoshi Murai; Hideto Miyoshi
The 1st China-Japan-Korea Workshop on Pesticide Science, Oct. 2009

Synthesis of photoreactive acetogenin and identification of its binding site in mitochondrial complex I
Masatoshi Murai; Koji Sekiguchi; Hideto Miyoshi
JSBBA Annual Meeting 2009, Mar. 2009

Synthesis of photoreactive acetogenin and identification of its binding site in mitochondrial complex I
Masatoshi Murai; Koji Sekiguchi; Hideto Miyoshi
Pesticide Society of Japan, 34th Annual Meeting, Mar. 2009

Characterization of inhibitor binding site in mitochondrial complex I using a quinazoline-type photoaffinity labeling probe
Masatoshi Murai; Koji Sekiguchi; Hideto Miyoshi
34th Japan Bioenergetics Group Meeting (JEBG 2008), Nov. 2008

Identification of the Inhibitor Binding Site(s) in Complex I by photoaffinity labeling
Masatoshi Murai; Naoya Ichimaru; Takao Yagi; Hideto Miyoshi
15th European Bioenergetics Conference (EBEC 2008), Jul. 2008

Probing the inhibitor binding site in mitochondrial complex I (NADH-ubiquinone oxidoreductase)
Masatoshi Murai; Takaaki Nishioka; Hideto Miyoshi
Pesticide Society of Japan, 33th Annual Meeting, Mar. 2008

Probing the Inhibitor Binding Site in Mitochondrial Complex I by Photoaffinity Labeling
Masatoshi Murai; Takaaki Nishioka; Hideto Miyoshi
JABBA Annual Meeting 2008, Mar. 2008

Mitochondrial complex I (NADH-Ubiquinone oxidoreductase); Probing the inhibitor binding site by photoaffinity labeling
Masatoshi Murai; Naoya Ichimaru; Takaaki Nishioka; Hideto Miyoshi
33th Japan Bioenergetics Group meeting (JEBG 2007), Nov. 2007

Identification of the binding site of Δlac-acetogenin by photoaffinity labeling
Masatoshi Murai; Naoya Ichimaru; Takaaki Nishioka; Hideto Miyoshi
JSBBA Annual Meeting 2007, Mar. 2007

Identification of the binding site of delta.lac acetogenin in mitochindrial complex I
Masatoshi Murai; Takaaki Nishioka; Hideto Miyoshi
JSBBA Annual Meeting 2007, Mar. 2007

Mode of inhibitory action of delta.lac-acetogenins, a new class of inhibitors of bovine heart mitochondrial complex I (NADH-ubiquinone oxidoreductase)
Masatoshi Murai; Hideto Miyoshi
32th Japan Bioenergetics Group Meeting (JBEG), Dec. 2006

Delta. lac acetogenin, a new class of inhibitors of bovine heart mitochondrial complex I
Masatoshi Murai; Naoya Ichimaru; Takaaki Nishioka; Hideto Miyoshi
JSBBA Kansai Annual Meeting 2006, Oct. 2006

Mode of inhibitory action of delta.lac-acetogenin on mitochondrial complex I
Masatoshi Murai; Naoya Ichimaru; Masato Abe; Takaaki Nishioka; Hideto Miyoshi
JSBBA Annual Meeting 2006, Mar. 2006

Screening of the proteins involved in uptake of ubiquinone in Saccharomyces cerevisiae using a synthetic ubiquinone probe
Seina Kuroda; Masatoshi Murai; Takahiro Masuya; Masahide Oku; Hideto Miyoshi
JSBBA 2022 annual meeting, 17 Mar. 2022

Comprehensive understanding of multiple actions of anticancer drug tamoxifen in isolated rat liver mitochondria
Yufu Unten; Masatoshi Murai; Tomoki Koshitaka; Kotaro Kitao; Osamu Shirai; Takahiro Masuya; Hideto Miyoshi
JSBBA annual meeting, 17 Mar. 2022

Site-specific chemical modification of Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae
Hinako Tanaka; Moe Ishikawa; Takahiro Masuya; Wataru Aoki; Masatoshi Murai; Blanca Barquera; Hideto Miyoshi
JSBBA 2022 annual meeting, 17 Mar. 2022

Diverse reaction manners of artificial ubiquinones in mitochondrial respiratory complex I
Shinpei Uno; Ryo Ikunishi; Takahiro Masuya; Kyoko Shinzawa-Ito; Masatoshi Murai; Hideto Miyoshi
JSBBA annual meeting, 17 Mar. 2022

The side chain of ubiquinone plays a critical role in Na+ translocation by NADH-ubiquinone oxidoreductase from Vibrio cholerae
Moe Ishikawa; Takahiro Masuya; Seina kuroda; Shinpei Uno; Tomoo Shiba; Ken Daniel Inaoka; Masatoshi Murai; Blanca Barquera; Hideto Miyoshi
JSBBA 2022 annual meeting, 17 Mar. 2022

Dynamic structural properties of the ubiquinone reaction cavity of mitochondrial respiratory complex I
Shinpei Uno; Ryo Ikunishi; Takahiro Masuya; Kyoko Shinzawa-Ito; Masatoshi Murai; Hideto Miyoshi
Pesticide Science Society of Japan 47th annual meeting, 08 Mar. 2022

The side chain of ubiquinone plays a critical role in Na+ translocation by the NADH-ubiquinone oxidoreductase from Vibrio cholerae
Moe Ishikawa; Takahiro Masuya; Seina Kuroda; Shinpei Uno; Tomoo Shiba; Masatoshi Murai; Blanca Barquera; Hideto Miyoshi
Pesticide Science Society of Japan 47th annual meeting, 08 Mar. 2022

Awards

Mar. 2019
Japan Society for Bioscience, Biotechnology and Agrochemistry, JSBBA Award for Young Scientists

External funds: Kakenhi

Mechanism of aggregation of ADP/ATP carrier induced by epoxycyclohexenedione (ECHD)-type inhibitor
Grant-in-Aid for Scientific Research (C)
Basic Section 38040:Bioorganic chemistry-related
Kyoto University
Masatoshi Murai
From 01 Apr. 2018, To 31 Mar. 2021, Project Closed
ミトコンドリア;ADP/ATP輸送体;ECHD類;ケミカルバイオロジー;阻害剤
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K05458/

Novel pathway of coenzyme Q biosynthesis
Grant-in-Aid for Scientific Research (B)
Shimane University
Makoto Kawamukai
From 01 Apr. 2017, To 31 Mar. 2021, Project Closed
coenzyme Q;ubiquinone;S. pombe;fission yeast;PHB;PABA;isoprenoid;コエンザイムQ;ユビキノン;分裂酵母;coenzymeQ;mitochondria;S. pome;ミトコンドリア;電子伝達系;抗酸化;イソプレノド
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17H03806/

Conversion of mitochondrial respiratory complex I to a light-driven proton pump by chemical modification
Grant-in-Aid for Challenging Exploratory Research
Kyoto University
Hideto Miyoshi
From 01 Apr. 2016, To 31 Mar. 2018, Project Closed
生物制御化学;ミトコンドリア呼吸鎖酵素;複合体-I;タンパク質化学修飾;ミトコンドリア;化学修飾;ミトコンドリア呼吸鎖;光スイッチ
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K14914/

Functional analysis of mitochondrial VDAC using synthetic ubiquinone probes
Grant-in-Aid for Scientific Research (C)
Kyoto University
MASATOSHI MURAI
From 01 Apr. 2015, To 31 Mar. 2018, Project Closed
ユビキノン;ミトコンドリア;VDAC;光親和性標識;ケミカルバイオロジー;膜透過性亢進;クリックケミストリー
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K07411/

Site-specific modification of respiratory complex I for single molecule analysis
Grant-in-Aid for Scientific Research (B)
Kyoto University
Hideto Miyoshi
From 01 Apr. 2014, To 31 Mar. 2017, Project Closed
生物制御化学;呼吸鎖複合体-I;化学修飾;ユビキノン;ミトコンドリア呼吸鎖;トシル化学;複合体-I;ミトコンドリア;呼吸鎖;呼吸鎖酵素
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26292060/

Molecular mechanism of mitochondrial proteins using ubiquinone as a cofactor
Grant-in-Aid for Young Scientists (B)
Kyoto University
Masatoshi MURAI
From 01 Apr. 2013, To 31 Mar. 2015, Project Closed
ユビキノン;Coq10;光親和性標識;ミトコンドリア;複合体-I;Click Chemistry;ユビキノン結合性タンパク質
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25850081/

Probing the intercellular traffic mechanism of ubiquinone using synthetic molecular probes
Grant-in-Aid for Young Scientists (B)
Kyoto University
Masatoshi MURAI
From 01 Apr. 2011, To 31 Mar. 2013, Project Closed
ユビキノン;ミトコンドリア;光親和性標識;Click Chemistry;複合体-I;出芽酵母
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780116/

Study on the function of ND1 subunit, a key subunit of respiratory complex I
Grant-in-Aid for Scientific Research (B)
Kyoto University
Hideto MIYOSHI
From 01 Apr. 2011, To 31 Mar. 2014, Project Closed
細胞機能調節物質;ミトコンドリア呼吸鎖酵素;複合体-I;アセトゲニン;光親和性標識;トシルケミストリー;ミトコンドリア;阻害剤;呼吸鎖複合体-I;ND1サブユニット;呼吸鎖阻害剤
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23380064/

Exploring the reaction mechanism of mitochondrial NADH-ubiquinone oxidoreductase (compelx I) using a specific inhibitor as a molecular probe
Grant-in-Aid for Research Activity Start-up
Kyoto University
Masatoshi MURAI
From 01 Apr. 2009, To 31 Mar. 2011, Project Closed
ミトコンドリア;複合体-I;キナゾリン;ユビキノン;光親和性標識;アセトゲニン;生物有機化学
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21880024/

Screening of the proteins involved in uptake of ubiquinone in Saccharomyces cerevisiae using synthetic ubiquinone probes
Grant-in-Aid for Scientific Research (B)
Basic Section 38040:Bioorganic chemistry-related
Kyoto University
村井正俊
From 01 Apr. 2022, To 31 Mar. 2025, Granted
ユビキノン（コエンザイムQ);ミトコンドリア;光親和性標識;出芽酵母;ユビキノン（コエンザイムQ）;ユビキノン;細胞内輸送;クリックケミストリー
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H02273/

Screening of the proteins involved in uptake of ubiquinone in Saccharomyces cerevisiae using synthetic ubiquinone probes
Grant-in-Aid for Scientific Research (B)
Basic Section 38040:Bioorganic chemistry-related
Kyoto University
村井正俊
From 01 Apr. 2022, To 31 Mar. 2025, Granted
ユビキノン（コエンザイムQ）;ミトコンドリア;光親和性標識;出芽酵母;ユビキノン（コエンザイムQ);ユビキノン;細胞内輸送;クリックケミストリー
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K23540/

External funds: others

Generation of “Light-driven” respiratory complex I through chemical modification
上原記念生命科学財団研究奨励金
From 01 Apr. 2018, To 31 Mar. 2019
村井正俊

Probing the structure and function of mitochondrial NADH-ubiquinone oxidoreductase (Complex I) by photoaffinity labeling technique
上原記念生命科学財団　研究奨励金
From 01 Apr. 2010, To 31 Mar. 2011
村井正俊

Last Updated :2025/04/24

Education

Teaching subject(s)

From 01 Apr. 2024, To 31 Mar. 2025
Chemistry of Biological Activity
CA19, Spring, Graduate School of Agriculture, 1

From 01 Apr. 2024, To 31 Mar. 2025
Biofunction Chemistry Seminar
CF09, Fall, Graduate School of Agriculture, 1.5

From 01 Apr. 2024, To 31 Mar. 2025
Biofunction Chemistry Seminar
CF09, Spring, Graduate School of Agriculture, 1.5

From 01 Apr. 2024, To 31 Mar. 2025
Experimental Course of Biofunction Chemistry
CG09, Spring, Graduate School of Agriculture, 3

From 01 Apr. 2024, To 31 Mar. 2025
Experimental Course of Biofunction Chemistry
CG09, Fall, Graduate School of Agriculture, 3

From 01 Apr. 2024, To 31 Mar. 2025
Laboratory Course in Organic Chemistry
5677, Fall, Faculty of Agriculture, 3

From 01 Apr. 2024, To 31 Mar. 2025
Introduction to Applied Life Science IV
5607, Fall, Faculty of Agriculture, 2

From 01 Apr. 2024, To 31 Mar. 2025
Bioorganic Chemistry I
5627, Spring, Faculty of Agriculture, 2

From 01 Apr. 2023, To 31 Mar. 2024
Chemistry of Biological Activity
CA19, Spring, Graduate School of Agriculture, 1

From 01 Apr. 2023, To 31 Mar. 2024
Laboratory Course in Organic Chemistry
5677, Year-long, Faculty of Agriculture, 3

From 01 Apr. 2023, To 31 Mar. 2024
Biofunction Chemistry Seminar2
CB36, Year-long, Graduate School of Agriculture, 3

From 01 Apr. 2023, To 31 Mar. 2024
Biofunction Chemistry Seminar1
CB35, Year-long, Graduate School of Agriculture, 3

From 01 Apr. 2023, To 31 Mar. 2024
Experimental Course of Biofunction Chemistry2
CC36, Year-long, Graduate School of Agriculture, 6

From 01 Apr. 2023, To 31 Mar. 2024
Experimental Course of Biofunction Chemistry1
CC35, Year-long, Graduate School of Agriculture, 6

From 01 Apr. 2023, To 31 Mar. 2024
Bioorganic Chemistry I
5627, Spring, Faculty of Agriculture, 2

From 01 Apr. 2023, To 31 Mar. 2024
Introduction to Applied Life Science IV
5607, Fall, Faculty of Agriculture, 2

From 01 Apr. 2022, To 31 Mar. 2023
Introduction to Applied Life Science IV
5607, Fall, Faculty of Agriculture, 2

From 01 Apr. 2022, To 31 Mar. 2023
Experimental Course of Biofunction Chemistry2
CC36, Year-long, Graduate School of Agriculture, 6

From 01 Apr. 2022, To 31 Mar. 2023
Experimental Course of Biofunction Chemistry1
CC35, Year-long, Graduate School of Agriculture, 6

From 01 Apr. 2022, To 31 Mar. 2023
Chemistry of Biological Activity
CA19, Spring, Graduate School of Agriculture, 1

From 01 Apr. 2022, To 31 Mar. 2023
Laboratory Course in Organic Chemistry
5677, Year-long, Faculty of Agriculture, 3

From 01 Apr. 2022, To 31 Mar. 2023
Biofunction Chemistry Seminar2
CB36, Year-long, Graduate School of Agriculture, 3

From 01 Apr. 2022, To 31 Mar. 2023
Biofunction Chemistry Seminar1
CB35, Year-long, Graduate School of Agriculture, 3

From Apr. 2011, To Mar. 2012
有機化学実験
Fall, 農学部

From Apr. 2011, To Mar. 2012
生物機能制御化学専攻実験
農学研究科

From Apr. 2011, To Mar. 2012
生物機能制御化学専攻演習
農学研究科

From Apr. 2012, To Mar. 2013
Laboratory Course in Organic Chemistry
Fall, 農学部

From Apr. 2013, To Mar. 2014
Laboratory Course in Organic Chemistry
Fall, 農学部

From Apr. 2013, To Mar. 2014
Chemistry of Biological Activity
Fall, 農学研究科

From Apr. 2013, To Mar. 2014
Biofunction Chemistry Seminar
Year-long, 農学研究科

From Apr. 2013, To Mar. 2014
Experimental Course of Biofunction Chemistry
Year-long, 農学研究科

From Apr. 2014, To Mar. 2015
Laboratory Course in Organic Chemistry
Fall, 農学部

From Apr. 2014, To Mar. 2015
Chemistry of Biological Activity
Spring, 農学研究科

From Apr. 2014, To Mar. 2015
Biofunction Chemistry Seminar
Year-long, 農学研究科

From Apr. 2014, To Mar. 2015
Experimental Course of Biofunction Chemistry
Year-long, 農学研究科

From Apr. 2015, To Mar. 2016
Laboratory Course in Organic Chemistry
Fall, 農学部

From Apr. 2015, To Mar. 2016
Experimental Course of Biofunction Chemistry1
Year-long, 農学研究科

From Apr. 2015, To Mar. 2016
Experimental Course of Biofunction Chemistry
Year-long, 農学研究科

From Apr. 2015, To Mar. 2016
Biofunction Chemistry Seminar1
Year-long, 農学研究科

From Apr. 2015, To Mar. 2016
Biofunction Chemistry Seminar
Year-long, 農学研究科

From Apr. 2015, To Mar. 2016
Chemistry of Biological Activity
Spring, 農学研究科

From Apr. 2015, To Mar. 2016
Fundamental Study on Agrochemicals
Spring, 全学共通科目

From Apr. 2016, To Mar. 2017
Laboratory Course in Organic Chemistry
Fall, 農学部

From Apr. 2016, To Mar. 2017
Experimental Course of Biofunction Chemistry1
Year-long, 農学研究科

From Apr. 2016, To Mar. 2017
Experimental Course of Biofunction Chemistry2
Year-long, 農学研究科

From Apr. 2016, To Mar. 2017
Experimental Course of Biofunction Chemistry
Year-long, 農学研究科

From Apr. 2016, To Mar. 2017
Biofunction Chemistry Seminar1
Year-long, 農学研究科

From Apr. 2016, To Mar. 2017
Biofunction Chemistry Seminar2
Year-long, 農学研究科

From Apr. 2016, To Mar. 2017
Biofunction Chemistry Seminar
Year-long, 農学研究科

From Apr. 2016, To Mar. 2017
Chemistry of Biological Activity
Spring, 農学研究科

From Apr. 2017, To Mar. 2018
Laboratory Course in Organic Chemistry
Fall, 農学部

From Apr. 2017, To Mar. 2018
Experimental Course of Biofunction Chemistry1
Year-long, 農学研究科

From Apr. 2017, To Mar. 2018
Experimental Course of Biofunction Chemistry2
Year-long, 農学研究科

From Apr. 2017, To Mar. 2018
Experimental Course of Biofunction Chemistry
Year-long, 農学研究科

From Apr. 2017, To Mar. 2018
Biofunction Chemistry Seminar1
Year-long, 農学研究科

From Apr. 2017, To Mar. 2018
Biofunction Chemistry Seminar2
Year-long, 農学研究科

From Apr. 2017, To Mar. 2018
Biofunction Chemistry Seminar
Year-long, 農学研究科

From Apr. 2017, To Mar. 2018
Chemistry of Biological Activity
Spring, 農学研究科

From Apr. 2018, To Mar. 2019
Laboratory Course in Organic Chemistry
Fall, 農学部

From Apr. 2018, To Mar. 2019
Experimental Course of Biofunction Chemistry1
Year-long, 農学研究科

From Apr. 2018, To Mar. 2019
Experimental Course of Biofunction Chemistry2
Year-long, 農学研究科

From Apr. 2018, To Mar. 2019
Biofunction Chemistry Seminar1
Year-long, 農学研究科

From Apr. 2018, To Mar. 2019
Biofunction Chemistry Seminar2
Year-long, 農学研究科

From Apr. 2018, To Mar. 2019
Chemistry of Biological Activity
Spring, 農学研究科

From Apr. 2019, To Mar. 2020
Introduction to Applied Life Science IV
Fall, 農学部

From Apr. 2019, To Mar. 2020
Laboratory Course in Organic Chemistry
Fall, 農学部

From Apr. 2019, To Mar. 2020
Experimental Course of Biofunction Chemistry1
Year-long, 農学研究科

From Apr. 2019, To Mar. 2020
Experimental Course of Biofunction Chemistry2
Year-long, 農学研究科

From Apr. 2019, To Mar. 2020
Biofunction Chemistry Seminar1
Year-long, 農学研究科

From Apr. 2019, To Mar. 2020
Biofunction Chemistry Seminar2
Year-long, 農学研究科

From Apr. 2019, To Mar. 2020
Chemistry of Biological Activity
Spring, 農学研究科

From Apr. 2020, To Mar. 2021
Scientific English
Spring, 農学部

From Apr. 2020, To Mar. 2021
Introduction to Applied Life Science IV
Fall, 農学部

From Apr. 2020, To Mar. 2021
Laboratory Course in Organic Chemistry
Year-long, 農学部

From Apr. 2020, To Mar. 2021
Experimental Course of Biofunction Chemistry1
Year-long, 農学研究科

From Apr. 2020, To Mar. 2021
Experimental Course of Biofunction Chemistry2
Year-long, 農学研究科

From Apr. 2020, To Mar. 2021
Biofunction Chemistry Seminar1
Year-long, 農学研究科

From Apr. 2020, To Mar. 2021
Biofunction Chemistry Seminar2
Year-long, 農学研究科

From Apr. 2020, To Mar. 2021
Chemistry of Biological Activity
Spring, 農学研究科

From Apr. 2021, To Mar. 2022
Basic Organic Chemistry I
Spring, 全学共通科目

From Apr. 2021, To Mar. 2022
Introduction to Applied Life Science IV
Fall, 農学部

From Apr. 2021, To Mar. 2022
Laboratory Course in Organic Chemistry
Year-long, 農学部

From Apr. 2021, To Mar. 2022
Experimental Course of Biofunction Chemistry1
Year-long, 農学研究科

From Apr. 2021, To Mar. 2022
Experimental Course of Biofunction Chemistry2
Year-long, 農学研究科

From Apr. 2021, To Mar. 2022
Biofunction Chemistry Seminar1
Year-long, 農学研究科

From Apr. 2021, To Mar. 2022
Biofunction Chemistry Seminar2
Year-long, 農学研究科

From Apr. 2021, To Mar. 2022
Chemistry of Biological Activity
Spring, 農学研究科

Last Updated :2025/04/24

Administration

School management (title, position)

From 01 Jan. 2022, To 31 Dec. 2023
オープンキャンパス委員会委員

From 10 Nov. 2016, To 12 Dec. 2016
放射性同位元素総合センター液体シンチレーションカウンター仕様策定委員会委員

Faculty management (title, position)

From 01 Apr. 2019, To 31 Mar. 2027
広報委員会　委員

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Murai, Masatoshi

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Profile

Language of Instruction

ID,URL

ORCID ID

J-Global ID

External link

Website(s) (URL(s))

researchmap URL

Research

Research Topics, Overview of the research

Research Topics

Overview of the research

Research Interests

Research Areas

Papers

Misc.

Presentations

Awards

External funds: Kakenhi

External funds: others

Education

Teaching subject(s)

Administration

School management (title, position)

Faculty management (title, position)

Academic, Social Contribution

Committee Memberships