Basic Information

Faculty

• 農学部

Academic Degree

• 修士（工学）(広島大学)
• 博士（工学）(広島大学)

ID,URL

• J-Global ID

  201501001523052201

researchmap URL

• https://researchmap.jp/mk1

Research

Research Areas

• Life sciences, Wood science
• Environmental science/Agricultural science, Environmental materials/recycling technology
• Life sciences, Molecular biology
• Life sciences, Applied microbiology

Papers

• Pleurotus ostreatus mek1 is essential for meiosis and basidiospore production

  Takehito Nakazawa; Takeshi Kobukata; Fuga Yamasaki; Junko Sugano; Minji Oh; Moriyuki Kawauchi; Yoichi Honda

  Fungal Biology, May 2025

  
• Trans-nuclei CRISPR/Cas9: safe approach for genome editing in the edible mushroom excluding foreign DNA sequences.

  Daishiro Koshi; Junko Sugano; Fuga Yamasaki; Moriyuki Kawauchi; Takehito Nakazawa; Minji Oh; Yoichi Honda

  Applied microbiology and biotechnology, 30 Dec. 2024

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• Role of putative APSES family transcription factor Swi6 in cell wall synthesis regulation in the agaricomycete Pleurotus ostreatus

  Hayase Kojima; Takaya Izumi; Moriyuki Kawauchi; Yuitsu Otsuka; Kenya Tsuji; Akira Yoshimi; Chihiro Tanaka; Shigekazu Yano; Takehito Nakazawa; Yoichi Honda

  Fungal Biology, Feb. 2025

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• Physiological function of hydrophobin Hydph16 in cell wall formation in agaricomycete Pleurotus ostreatus

  Junxian Han; Moriyuki Kawauchi; Yuki Terauchi; Kenya Tsuji; Akira Yoshimi; Chihiro Tanaka; Takehito Nakazawa; Yoichi Honda

  Fungal Genetics and Biology, Nov. 2024

  
• Marker recycling in Lentinula edodes via 5-fluoroorotic acid counter-selection

  Takuma Narutaki; Ayane Kamiya; Yuma Tsujimoto; Masataka Naruse; Shota Nishida; Moriyuki Kawauchi; Yoichi Honda; Hisatoshi Kamitsuji; Toshitsugu Sato; Takuya Sumita; Kosuke Izumitsu; Toshikazu Irie

  FEMS Microbiology Letters, 09 Jan. 2024

  
• (Chemical and Pharmaceutical Bulletin (2010) 58, (934))

  Kawauchi, M.; Arima, T.; Shirota, O.; Sekita, S.; Nakane, T.; Takase, Y.; Kuroyanagi, M.

  Chemical and Pharmaceutical Bulletin, 2010

  
• Disruption of the pkac2 gene in Pleurotus ostreatus alters cell wall structures and enables mycelial dispersion in liquid culture.

  Yuitsu Otsuka; Moriyuki Kawauchi; Kai Yoshizawa; Saori Endo; Kim Schiphof; Kenya Tsuji; Akira Yoshimi; Chihiro Tanaka; Shigekazu Yano; Vladimir Elisashvili; Takehito Nakazawa; Toshikazu Irie; Yoichi Honda

  FEMS microbiology letters, 20 Nov. 2024

  
• Effects of hap2 deletion on mnp/vp transcription in Pleurotus ostreatus grown on lignocellulosic substrates.

  Keita Kayama; Takehito Nakazawa; Iori Yamaguchi; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Applied microbiology and biotechnology, 13 Nov. 2024

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• Putative APSES family transcription factor mbp1 plays an essential role in regulating cell wall synthesis in the agaricomycete Pleurotus ostreatus.

  Hayase Kojima; Moriyuki Kawauchi; Yuitsu Otsuka; Kim Schiphof; Kenya Tsuji; Akira Yoshimi; Chihiro Tanaka; Shigekazu Yano; Takehito Nakazawa; Yoichi Honda

  Fungal genetics and biology : FG & B, 04 Oct. 2024

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• Functional analysis of basidiomycete specific chitin synthase genes in the agaricomycete fungus Pleurotus ostreatus

  Kim Schiphof; Moriyuki Kawauchi; Kenya Tsuji; Akira Yoshimi; Chihiro Tanaka; Takehito Nakazawa; Yoichi Honda

  Fungal Genetics and Biology, Jun. 2024

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• Identification of two genes essential for basidiospore formation during the postmeiotic stages in Pleurotus ostreatus.

  Takeshi Kobukata; Takehito Nakazawa; Fuga Yamasaki; Junko Sugano; Minji Oh; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Fungal genetics and biology : FG & B, 17 Mar. 2024

  
• Pleurotus ostreatus as a model mushroom in genetics, cell biology, and material sciences.

  Takehito Nakazawa; Moriyuki Kawauchi; Yuitsu Otsuka; Junxian Han; Daishiro Koshi; Kim Schiphof; Lucía Ramírez; Antonio G Pisabarro; Yoichi Honda

  Applied microbiology and biotechnology, 19 Feb. 2024

  
• Experimental evidence that lignin-modifying enzymes are essential for degrading plant cell wall lignin by Pleurotus ostreatus using CRISPR/Cas9

  Takehito Nakazawa; Iori Yamaguchi; Yufan Zhang; Chinami Saka; Hongli Wu; Keita Kayama; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Environmental Microbiology, Oct. 2023

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• Visualizing organelles with recombinant fluorescent proteins in the white-rot fungus Pleurotus ostreatus

  Kazuhiro Kurebayashi; Takehito Nakazawa; Shivani; Yuta Higashitarumizu; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Fungal Biology, 01 Oct. 2023

  
• Physiological function of hydrophobin Vmh3 in lignin degradation by white-rot fungus Pleurotus ostreatus

  Junxian Han; Moriyuki Kawauchi; Yuki Terauchi; Akira Yoshimi; Chihiro Tanaka; Takehito Nakazawa; Yoichi Honda

  Letters in Applied Microbiology, Apr. 2023

  
• Features of disruption mutants of genes encoding for hydrophobin Vmh2 and Vmh3 in mycelial formation and resistance to environmental stress in Pleurotus ostreatus.

  Junxian Han; Moriyuki Kawauchi; Kim Schiphof; Yuki Terauchi; Akira Yoshimi; Chihiro Tanaka; Takehito Nakazawa; Yoichi Honda

  FEMS microbiology letters, 17 Jan. 2023

  
• The lignin-degrading abilities of Gelatoporia subvermispora gat1 and pex1 mutants generated via CRISPR/Cas9.

  Takehito Nakazawa; Chikako Inoue; Ryota Morimoto; Dong Xuan Nguyen; Dapeng Bao; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Environmental microbiology, 23 Mar. 2023

  
• Double-gene targeting with pre-assembled Cas9 ribonucleoprotein for safe genome editing in the edible mushroom Pleurotus ostreatus.

  Tatpong Boontawon; Takehito Nakazawa; Yeon-Jae Choi; Hyeon-Su Ro; Minji Oh; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  FEMS microbiology letters, 22 Feb. 2023

  
• Introducing multiple-gene mutations in Pleurotus ostreatus using a polycistronic tRNA and CRISPR guide RNA strategy.

  Haibo Xu; Takehito Nakazawa; Yufan Zhang; Minji Oh; Dapeng Bao; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  FEMS microbiology letters, 09 Nov. 2022

  
• Gene targeting of dikaryotic Pleurotus ostreatus nuclei using the CRISPR/Cas9 system.

  Fuga Yamasaki; Takehito Nakazawa; Minji Oh; Dapeng Bao; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  FEMS microbiology letters, 24 Aug. 2022

  
• CRISPR/Cas9 using a transient transformation system in Ceriporiopsis subvermispora.

  Takehito Nakazawa; Chikako Inoue; Dong Xuan Nguyen; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Applied microbiology and biotechnology, 29 Jul. 2022

  
• Marker-free genome editing in the edible mushroom, Pleurotus ostreatus, using transient expression of genes required for CRISPR/Cas9 and for selection

  Daishiro Koshi; Hiroki Ueshima; Moriyuki Kawauchi; Takehito Nakazawa; Masahiro Sakamoto; Mana Hirata; Kosuke Izumitsu; Takuya Sumita; Toshikazu Irie; Yoichi Honda

  Journal of Wood Science, Dec. 2022

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• Cell Wall Integrity and Its Industrial Applications in Filamentous Fungi

  Akira Yoshimi; Ken Miyazawa; Moriyuki Kawauchi; Keietsu Abe

  Journal of Fungi, 23 Apr. 2022

  
• Overexpressing Pleurotus ostreatus rho1b results in transcriptional upregulation of the putative cellulolytic enzyme-encoding genes observed in ccl1 disruptants

  Nozomi Okuda; Takehito Nakazawa; Masato Horii; Hongli Wu; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Environmental Microbiology, Nov. 2021

  
• Functional analyses of Pleurotus ostreatus pcc1 and clp1 using CRISPR/Cas9

  Tatpong Boontawon; Takehito Nakazawa; Masato Horii; Masami Tsuzuki; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Fungal Genetics and Biology, Sep. 2021, Peer-reviewed

  
• Gene targeting using pre-assembled Cas9 ribonucleoprotein and split-marker recombination in Pleurotus ostreatus

  Tatpong Boontawon; Takehito Nakazawa; Haibo Xu; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  FEMS Microbiology Letters, 07 Jul. 2021, Peer-reviewed

  
• Efficient genome editing with CRISPR/Cas9 in Pleurotus ostreatus.

  Tatpong Boontawon; Takehito Nakazawa; Chikako Inoue; Keishi Osakabe; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  AMB Express, 20 Feb. 2021, Peer-reviewed

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• Comparative transcriptional analyses of Pleurotus ostreatus mutants on beech wood and rice straw shed light on substrate-biased gene regulation.

  Hongli Wu; Takehito Nakazawa; Haibo Xu; Ruiheng Yang; Dapeng Bao; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Applied microbiology and biotechnology, Feb. 2021, Peer-reviewed

  
• A promoter assay system using gene targeting in agaricomycetes Pleurotus ostreatus and Coprinopsis cinerea

  Dong Xuan Nguyen; Takehito Nakazawa; Genki Myo; Chikako Inoue; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Journal of Microbiological Methods, Dec. 2020, Peer-reviewed

  
• Characterization of the effects of terminators and introns on recombinant gene expression in the basidiomycete Ceriporiopsis subvermispora

  Dong Xuan Nguyen; Emi Nishisaka; Moriyuki Kawauchi; Takehito Nakazawa; Masahiro Sakamoto; Yoichi Honda

  Journal of Microbiology, Dec. 2020, Peer-reviewed

  
• Development of CRISPR-Cas13a-based antimicrobials capable of sequence-specific killing of target bacteria

  Kotaro Kiga; Xin Ee Tan; Rodrigo Ibarra-Chávez; Shinya Watanabe; Yoshifumi Aiba; Yusuke Sato’o; Feng Yu Li; Teppei Sasahara; Bintao Cui; Moriyuki Kawauchi; Tanit Boonsiri; Kanate Thitiananpakorn; Yusuke Taki; Aa Haeruman Azam; Masato Suzuki; José R. Penadés; Longzhu Cui

  Nature Communications, 01 Dec. 2020, Peer-reviewed

  
• Corrigendum: Composition and Diversity of CRISPR-Cas13a Systems in the Genus Leptotrichia (Frontiers in Microbiology, (2019), 10, 10.3389/fmicb.2019.02838)

  Shinya Watanabe; Bintao Cui; Kotaro Kiga; Yoshifumi Aiba; Xin Ee Tan; Yusuke Sato'o; Moriyuki Kawauchi; Tanit Boonsiri; Kanate Thitiananpakorn; Yusuke Taki; Fen Yu Li; Aa Haeruman Azam; Yumi Nakada; Teppei Sasahara; Longzhu Cui

  Frontiers in Microbiology, 12 Feb. 2020

  
• Composition and Diversity of CRISPR-Cas13a Systems in the Genus Leptotrichia

  Shinya Watanabe; Bintao Cui; Kotaro Kiga; Yoshifumi Aiba; Xin-Ee Tan; Yusuke Sato’o; Moriyuki Kawauchi; Tanit Boonsiri; Kanate Thitiananpakorn; Yusuke Taki; Fen-Yu Li; Aa Haeruman Azam; Yumi Nakada; Teppei Sasahara; Longzhu Cui

  Frontiers in Microbiology, 10 Dec. 2019, Peer-reviewed

  
• Complete genome sequence of the methicillin-resistant staphylococcus aureus Strain JMUB3031, isolated from a patient with fatal community-acquired pneumonia

  Bintao Cui; Shinya Watanabe; Yusuke Satoo; Fumiya Nihashi; Yoshifumi Aiba; Kotaro Kiga; Teppei Sasahara; Xin Ee Tan; Moriyuki Kawauchi; Tanit Boonsiri; Kanate Thitiananpakorn; Yusuke Taki; Feng Yu Li; Shiro Imokawa; Longzhu Cui

  Microbiology Resource Announcements, 24 Jan. 2019, Peer-reviewed

  
• Molecular cloning and transcriptional analysis of WRKY and solavetivone biosynthetic genes in the hairy roots of Hyoscyamus albus

  Moriyuki Kawauchi; Toshi Hide Arima; Masanori Kuroyanagi

  Plant Gene, 01 Mar. 2016, Peer-reviewed

  
• Development of a promoter shutoff system in Aspergillus oryzae using a sorbitol-sensitive promoter

  Ken Oda; Shiho Terado; Rieko Toyoura; Hisashi Fukuda; Moriyuki Kawauchi; Kazuhiro Iwashita

  Bioscience, Biotechnology and Biochemistry, 2016, Peer-reviewed

  
• Functional analysis of histone deacetylase and its role in stress response, drug resistance and solid-state cultivation in Aspergillus oryzae

  Moriyuki Kawauchi; Kazuhiro Iwashita

  Journal of Bioscience and Bioengineering, Aug. 2014, Peer-reviewed

  
• Molecular cloning and characterization of coumarin glucosyltransferase in hairy roots of Pharbitis nil (Ipomoea nil)

  Hideaki Kanoh; Moriyuki Kawauchi; Masanori Kuroyanagi; Toshihide Arima

  Plant Biotechnology, 2014, Peer-reviewed

  
• Fungus-specific sirtuin HstD coordinates secondary metabolism and development through control of LaeA

  Moriyuki Kawauchi; Mika Nishiura; Kazuhiro Iwashita

  Eukaryotic Cell, Aug. 2013, Peer-reviewed

  
• Production of sesquiterpene-type phytoalexins by hairy roots of Hyoscyamus albus co-treated with cupper sulfate and methyl jasmonate.

  Moriyuki Kawauchi; Toshihide Arima; Osamu Shirota; Setsuko Sekita; Takahisa Nakane; Yoichi Takase; Masanori Kuroyanagi

  Chemical & pharmaceutical bulletin, Jul. 2010, Peer-reviewed

  

Misc.

• Development of CRISPR/Cas9 based trans-nuclei genome editing method for safe mushroom molecular breeding technology using the edible mushroom Pleurotus ostreatus

  越大志朗; 菅野純子; 河内護之; 中沢威人; 坂本正弘; OH Minji; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2024

  
• Development of a high-density liquid culture strain of the white-rot fungus Pleurotus ostreatus

  大塚唯; 河内護之; 辻健也; 吉見啓; 吉見啓; 田中千尋; 田中千尋; 矢野成和; 入江俊一; 中沢威人; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2024

  
• Developing a protocol for mycelial material production using the white-rot fungus Pleurotus ostreatus

  小島隼星; KIM Schiphof; 河内護之; 辻健也; 田中千尋; 田中千尋; JEONG Yong-Hyeon; SHIN Hyun-Jae; 中沢威人; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2024

  
• Functional analysis of highly expressed hydrophobin hydph16 during wood degradation in the white-rot fungus Pleurotus ostreatus

  HAN Junxian; 河内護之; 辻健也; 寺内裕貴; 吉見啓; 吉見啓; 田中千尋; 田中千尋; 中沢威人; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2024

  
• Functional analysis of the transcription factor Swi6, which contributes to the regulation of cell wall synthesis in the white-rot fungus, Pleurotus ostreatus.

  和泉貴哉; 小島隼星; 大塚唯; 河内護之; 辻健也; 吉見啓; 田中千尋; 田中千尋; 矢野成和; 中沢威人; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2024

  
• Analysis of physiological functions of the β-glucan synthase gene of Pleurotus ostreatus for elucidation of cell wall structure of white-rot fungi

  河内護之; 名和義順; 大塚唯; KIM Schiphof; WAN Y.; 辻健也; 吉見啓; 吉見啓; 田中千尋; 田中千尋; 中沢威人; 矢野成和; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2024

  
• 細胞壁多糖三重染色法の開発により見えてきた木材腐朽菌にユニークな細胞壁構造

  大塚唯; 河内護之; 辻健也; 吉見啓; 田中千尋; 中沢威人; 矢野成和; 本田与一

  応用糖質科学, 2024

  
• Experimental evidence that lignin-modifying enzymes are essential for degrading plant cell wall lignin by Pleurotus ostreatus using CRISPR/Cas9.

  Takehito Nakazawa; Iori Yamaguchi; Yufan Zhang; Chinami Saka; Hongli Wu; Keita Kayama; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Environmental microbiology, 22 May 2023

  
• Physiological function of hydrophobin Vmh3 in lignin degradation by white-rot fungus Pleurotus ostreatus.

  Junxian Han; Moriyuki Kawauchi; Yuki Terauchi; Akira Yoshimi; Chihiro Tanaka; Takehito Nakazawa; Yoichi Honda

  Letters in applied microbiology, 03 Apr. 2023

  
• Characterization of cell wall synthesis regulatory transcription factors Mbp1 and Rlm1 in basidiomycete Pleurotus ostreatus

  小島隼星; 亀井志乃; 名和義順; 大塚唯; 河内護之; 寺内裕貴; 吉見啓; 田中千尋; 矢野成和; 中沢威人; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2023

  
• Analysis of xylan synthase genes in grasses

  坂本正弘; 長谷川智彦; 河内護之; 中沢威人; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2023

  
• 細胞壁多糖三重染色法の開発により見えてきた木材腐朽菌にユニークな細胞壁構造

  大塚唯; 河内護之; 辻健也; 吉見啓; 田中千尋; 中沢威人; 矢野成和; 本田与一

  応用糖質科学, 2023

  
• Unique cell wall structure of basidiomycete focused on α-1,3-glucan in Pleurotus ostreatus

  河内護之; 名和義順; 大塚唯; 寺内裕貴; 吉見啓; 田中千尋; 阿部啓悦; 代田修; 矢野成和; 中沢威人; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2023

  
• 木材腐朽菌の細胞壁合成機構の理解を目的としたグルカン合成酵素の解析

  河内護之; 名和義則; 大塚唯; 辻健也; 吉見啓; 田中千尋; 阿部啓悦; 矢野成和; 中沢威人; 本田与一

  応用糖質科学, 2023

  
• Identification and functional analysis of β-glucan synthase genes in Pleurotus ostreatus

  名和義順; 大塚唯; 河内護之; 寺内裕貴; 吉見啓; 田中千尋; 矢野成和; 中沢威人; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2023

  
• Isolation of genes essential for basidiospore production in Pleurotus ostreatus using transcriptome analysis and CRISPR/Cas9

  小深田剛士; 山崎風雅; 中沢威人; 菅野純子; OH Minji; 河内護之; 坂本正弘; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2023

  
• Histone H3K36 methylation affects indirectly transcriptional alternation of cellulose degrading enzyme genes in Pleurotus ostreatus

  奥田希実; 中沢威人; 香山渓太; 河内護之; 坂本正弘; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2022

  
• A marker-free genome editing technique via transient gene expression in an edible mushroom, Pleurotus ostreatus.

  越大志朗; 河内護之; 入江俊一; 中沢威人; 坂本正弘; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2022

  
• Identification of genes which are involved in lacking the outer layer of ECM in Bipolaris maydis

  佐波雅史; 辻健也; 吉見啓; 本田与一; 河内護之; 田中千尋

  日本植物病理学会大会プログラム・講演要旨予稿集, 2022

  
• Function of an autonomously replicating sequence AMA1 in the basidiomycete Pleurotus ostreatus and its application to genome editing

  越大志朗; 河内護之; 中沢威人; 坂本正弘; 本田与一

  日本農芸化学会大会講演要旨集(Web), 2022

  
• Identification and functional analysis of α-1,3-glucan synthase gene in Pleurotus ostreatus

  名和義順; 河内護之; 中沢威人; 坂本正弘; 阿部敬悦; 田中千尋; 吉見啓; 本田与一

  日本農芸化学会大会講演要旨集(Web), 2022

  
• Fluorescence imaging visualizes the distinct cell wall structures of Basidiomycetes and Ascomycetes

  大塚唯; 山田夕月; 辻健也; 河内護之; 中沢威人; 吉見啓; 田中千尋; 坂本正弘; 矢野成和; 本田与一

  日本分子生物学会年会プログラム・要旨集(Web), 2022

  
• Evaluation of ecological role of secondary metabolism of Aspergillus nidulans in a soil environment

  高田万里奈; 河内護之; 大西康夫; 大西康夫; 妹尾啓史; 妹尾啓史; 清水公徳; 浦山俊一; 浦山俊一; 萩原大祐; 萩原大祐

  日本農芸化学会大会講演要旨集(Web), 19 Mar. 2021

  
• Roles of CCAAT-boxes in transcription of ligninolytic enzyme genes in Pleurotus ostreatus on lignocellulose

  中沢威人; 坂知奈美; 山本太一; 河内護之; 坂本正弘; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2021

  
• A transient transformation and its application to gneome editing system in pleurotus ostreatus

  越大志朗; 河内護之; 入江俊一; 中沢威人; 坂本正弘; 本田与一

  日本分子生物学会年会プログラム・要旨集(Web), 2021

  
• Evaluation of the ecological role of Aspergillus nidulans in the soil culture system

  Marina Takata; Moriyuki Kawauchi; Yasuo Ohnishi; Keishi Senoo; Kiminori Shimizu; Syun-ichi Urayama; Daisuke Hagiwara

  IUMS 2020 (International Union of Microbiological Societies Congresses), 12 Oct. 2020

  
• 土壌培養におけるモデル糸状菌Aspergillus nidulansの生態と土壌微生物叢との相互作用の解析

  高田万里奈; 河内護之; 大西康夫; 妹尾啓史; 清水公徳; 浦山俊一; 萩原大祐

  日本土壌微生物学会2020年度大会, 15 Jun. 2020

  
• 土壌培養におけるAspergillus nidulansの生態と土壌微生物叢との相互作用の解析

  高田万里奈; 河内護之; 大西康夫; 妹尾啓史; 清水公徳; 浦山俊一; 萩原大祐

  第19回糸状菌分子生物学コンファレンス, 06 Nov. 2019

  
• 土壌培養におけるモデル糸状菌Aspergillus nidulansのトランスクリプト−ム解析

  高田万里; 河内護之; 大西康夫; 妹尾啓史; 清水公徳; 浦山俊一; 萩原大祐

  日本土壌微生物学会2019年度大会, 15 Jun. 2019

  
• Development of phage genome assembly system using yeast

  河内護之; 氣駕恒太朗; LI Feng-Yu; BOONSIRI Tanit; TAN Xin Ee; 佐藤祐介; 相羽由詞; THITIANANPAKORN Kanate; 渡邊真弥; CUI Longzhu

  日本細菌学雑誌(Web), 23 Apr. 2019

  
• Isolation of the broad-host range phages from lysogenic Pseudomonas aeruginosa strains

  渡邊真弥; LI Feng-Yu; 氣駕恒太朗; 相羽由詞; 佐藤祐介; TAN Xin Ee; 河内護之; BOONSIRI Tanit; KANATE Thitiananpakorn; CUI Longzhu

  日本細菌学雑誌(Web), Mar. 2019

  
• 殺菌キメラファージの開発 酵母を利用したキメラファージ合成技術の開発

  河内 護之; 氣駕 恒太朗; 李 峰宇; Boonsiri Tanit; Tan Xin Ee; 佐藤 祐介; 相羽 由詞; Thitiananpakorn Kanate; 渡邊 真弥; 崔 龍洙

  日本細菌学雑誌, Mar. 2019

  
• AMR bacteria collection and isolation and identification of MRSA phage

  相羽由詞; TAN Xin Ee; THITIANANPAKORN Kanate; 渡邊真弥; 氣駕恒太朗; 佐藤祐介; BOONSIRI Tanit; LI Feng-Yu; 河内護之; CUI Longzhu

  日本細菌学雑誌(Web), Mar. 2019

  
• Establishment of the bacterial infection models of Galleria mellonella and mouse for phage therapy

  佐藤祐介; LI Feng-Yu; 氣駕恒太朗; 渡邊真弥; 相羽由詞; 河内護之; TAN Xin Ee; BOONSIRI Tanit; THITIANANPAKORN Kanate; CUI Longzhu

  日本細菌学雑誌(Web), Mar. 2019

  
• Isolation and characterization of the broad-host range phage from lysogenic E. coli and sewage

  LI Feng-Yu; 氣駕恒太朗; 渡邊真弥; 佐藤祐介; 相羽由詞; 河内護之; TAN Xin Ee; BOONSIRI Tanit; THITIANANPAKORN Kanate; CUI Longzhu

  日本細菌学雑誌(Web), Mar. 2019

  
• Generation of bactericidal chimeric phages using SaPI-phage system of Staphylococcus aureus

  TAN Xin Ee; 氣駕恒太朗; 渡邊真弥; 佐藤祐介; 相羽由詞; 河内護之; THITIANANPAKORN Kanate; CHAVEZ Victor Rodrigo Ibarra; PENADES Jose R; CUI Longzhu

  日本細菌学雑誌(Web), Mar. 2019

  
• MRSAにおけるdaptomycinとvancomycinに対する交差耐性の機序(Mechanisms of cross-resistant to daptomycin and vancomycin in MRSA)

  Thitiananpakorn Kanate; 相羽 由詞; Tan Xin Ee; 渡邊 真弥; 氣駕 恒太朗; 佐藤 祐介; 河内 護之; Boonsiri Tanit; Cui Bintao; 崔 龍洙

  日本細菌学雑誌, Mar. 2019

  
• Characterization of CRISPR-Cas13 system from Leptotrichiaceae

  CUI Bintao; 渡邊真弥; 氣駕恒太朗; 相羽由詞; 河内護之; BOONSIRI Tanit; THITIANANPAKORN Kanate; 佐藤祐介; TAN Xin Ee; CUI Longzhu

  日本細菌学雑誌(Web), Mar. 2019

  
• Soil cultivation system for physiological analysis of Streptomyces griseus

  Moriyuki Kawauchi; Takeaki Tezuka; Keishi Senoo; Yasuo Ohnishi

  IUMS 2017 (International Union of Microbiological Societies Congresses), 23 Jul. 2017

  
• Soil Cultivation System for Physiological Analysis of Streptomyces griseus

  Moriyuki Kawauchi; Takeaki Tezuka; Keishi Senoo; Yasuo Ohnishi

  18th International Symposium on the Biology of Actinomycetes, 23 May 2017

  
• 土壌での放線菌の生理・生態解析を目指したStreptomyces griseusの滅菌土壌培養

  河内護之; 手塚武揚; 妹尾啓史; 大西康夫

  日本農芸化学会大会講演要旨集(Web), 05 Mar. 2017

  
• モロミックス解析による清酒中のアルギニン・アグマチンの生産性に関連する遺伝子の同定

  嶋本孝平; 河内護之; 織田健; 岩下和裕

  日本農芸化学会大会講演要旨集(Web), 05 Mar. 2017

  
• 土壌での放線菌の生理・生態解析を目指したStreptomyces griseusの滅菌土壌培養

  河内護之; 手塚武揚; 妹尾啓史; 大西康夫

  日本微生物生態学会大会(Web), 2017

  
• 滅菌土壌を用いた放線菌Streptomyces griseusの培養―土壌における放線菌の生理・生態解析を目指して―

  河内護之; 手塚武揚; 妹尾啓史; 大西康夫

  日本放線菌学会大会講演要旨集, 08 Sep. 2016

  
• 麹菌の2次代謝遺伝子発現を制御するAospt3とAohstDとの関係

  HIROSE MASATO; KAWAUCHI MORIYUKI; ODA KEN; IWASHITA KAZUHIRO

  日本農芸化学会大会講演要旨集(Web), 05 Mar. 2015

  
• 新規二次代謝制御因子Aospt3の機能解析

  HIROSE MASATO; KAWAUCHI MORIYUKI; ODA KEN; IWASHITA KAZUHIRO

  日本農芸化学会大会講演要旨集(Web), 05 Mar. 2014

  
• Fungal-specific sirtuin type HDAC HstD coordinates the secondary metabolism and development through the LaeA

  河内護之; 廣瀬雅人; 岩下和裕

  第35回日本分子生物学会年会, Dec. 2013

  
• Histone deacetylase HstDとLaeAのジェネティックインタラクション

  河内護之; 廣瀬雅人; 岩下和裕

  第12回糸状菌分子生物学会, Nov. 2013

  
• Fungal-specific sirtuin HstD coordinates the secondary metabolism and development via the LaeA

  Moriyuki Kawauchi

  27th Fungal genetics conference, 12 Mar. 2013

  
• 麹菌必須遺伝子の解析のためのプロモーターシャットオフシステムの開発

  TERADO SHIHO; SHIMAHARA TOMOKO; TOYOURA RIEKO; KAWAUCHI MORIYUKI; IWASHITA KAZUHIRO

  日本農芸化学会大会講演要旨集(Web), 05 Mar. 2013

  
• 4Fp14 Analysis of genetic interaction between histone deacetylase (HDAC) AoHst4 and LaeA

  Kawauchi Moriyuki; Hirose Masato; Iwashita Kazuhiro

  日本生物工学会大会講演要旨集, 25 Sep. 2012

  
• ヒヨス(Hyoscyamus albus)毛状根によるファイトアレキシンの生産と関連遺伝子

  KAWAUCHI MORIYUKI; KUROYANAGI MASANORI; SHIROTA OSAMU; ARIMA TOSHIHIDE

  天然有機化合物討論会講演要旨集, 02 Sep. 2011

  
• ヒストン制御関連遺伝子破壊株の醸造上の特性について

  KAWACHI MORIYUKI; NISHIURA MIKA; ISOGAI ATSUKO; IWASHITA KAZUHIRO

  日本醸造学会大会講演要旨集, 31 Aug. 2011

  
• 麹菌Histone deacetylase(HDAC)破壊株の表現型解析

  KAWACHI MORIYUKI; NISHIURA MIKA; IWASHITA TOMOHIRO; IWASHITA TOMOHIRO; YAMADA OSAMU

  日本農芸化学会大会講演要旨集, 05 Mar. 2011

  
• 麹菌のソルビトール応答遺伝子の機能解析とその応用

  SHIMAHARA AKIKO; TOYOURA RIEKO; KAWACHI MORIYUKI; IWASHITA KAZUHIRO; YAMADA OSAMU

  日本農芸化学会大会講演要旨集, 05 Mar. 2011

  
• Studies on Glucosylation of Phenolic Compounds by Morning Glory Hairy Roots and Related Enzymes

  Kanoh Hideki; Kuroyanagi Masanori; Kawauchi Moriyuki; Arima Toshihide; Yaoya Sayaka; Nakane Takahisa

  天然有機化合物討論会講演要旨集, 01 Sep. 2010

  
• ヒヨス毛状根によるセスキテルペン生産と関連遺伝子のクローニング

  KAWACHI MORIYUKI; ARIMA TOSHIHIDE; KUROYANAGI MASANORI

  日本農芸化学会大会講演要旨集, 05 Mar. 2009

  
• Phytoalexins from Hairy Roots of Hyoscyamus albus Co-treated with Copper Sulfate and Methyl Jasmonate

  河内護之; 有馬寿英; 代田修; 中根孝久; 黒柳正典

  香料・テルペンおよび精油化学に関する討論会講演要旨集, 2008

  
• Phenotypic analyses of the gene disruption strains of hydrophobin RolA and HypB in Aspergillus oryzae

  寺内裕貴; 辻健也; 吉見啓; 田中千尋; 田中千尋; 本田与一; 河内護之

  日本菌学会大会講演要旨集, 2022

  
• Examination of the participation of four putative hydrophobins in the hyphal surface hydrophobicity in Bipolaris maydis.

  吉田裕史; 佐波雅史; 寺内裕貴; 田中千尋; 本田与一; 河内護之; 吉見啓

  日本植物病理学会報, 2021

  
• Loss of pathogenicity in Bipolaris maydis mutants deficient in surface hydrophobicity.

  吉田裕史; 佐波雅史; 寺内裕貴; 田中千尋; 本田与一; 河内護之; 吉見啓

  日本植物病理学会報, 2021

  
• Identification of SSPs specific to several carbon substrates in Aspergillus oryzae and phytopathogenic filamentous fungi

  寺内裕貴; 寺内裕貴; 吉田裕史; 田中千尋; 田中千尋; 本田与一; 河内護之; 吉見啓

  日本農芸化学会大会講演要旨集(Web), 2021

  
• Functional analysis of the vegetative mycelium hydrophobin Vmh3 in the white rot fungus Pleurotus ostreatus

  HAN J.; 河内護之; 寺内裕貴; 吉見啓; 田中千尋; 中沢威人; 坂本正弘; 本田与一

  日本菌学会大会講演要旨集, 2022

  
• Identification of Xylan-specific SSPs from Aspergillus oryzae and analysis of phenotype in A. oryzae by disruption of SSPs

  寺内裕貴; 寺内裕貴; 田中千尋; 田中千尋; 本田与一; 河内護之; 吉見啓

  日本菌学会大会講演要旨集, 2021

  

Presentations

• Cell wall engineering of the mushroom for the eco-friendly material development

  Moriyuki Kawauchi; Yuitsu Otsuka; Kim Schiphof; Hayase Kojima; Shigekazu Yano; Kenya Tsuji; Akira Yoshimi; Chihiro Tanaka; Takehito Nakazawa; Yoichi Honda

  Asian Mycological Congress 2023, 13 Oct. 2023, Invited

  
• Recent progress of cell wall research in Pleurotus ostreatus

  河内護之

  ナバーラ公立大学セミナー, 04 Jul. 2023, Invited

  
• Cell Wall Editing for the Development of Beneficial Mushroom

  河内護之

  The 1st International Conference on Mycological Science and Engineering, 19 Feb. 2023, Invited

  
• Cell Wall Editing for the Development of Sustainable Mushroom Materials

  Moriyuki Kawauchi

  国立慶尚大学特別セミナー, 04 Nov. 2022, Invited

  
• 麹菌のエピジェネティックな二次代謝と分化の制御機構

  河内護之

  平成27年度清酒酵母麹研究会, 05 Oct. 2016, Invited

  
• 糸状菌の二次代謝と分化のコーディネーターLaeAの制御因子

  河内護之

  第18回真核微生物交流会, 12 Jun. 2015, Invited

  
• 2S-Dp02 Control mechanism of secondary metabolism by the fungal-specific epigenetic regulator

  Kawauchi Moriyuki; Iwashita Kazuhiro

  日本生物工学会大会講演要旨集, 25 Aug. 2013, Invited

  

Industrial Property Rights

• 特許第5995218号, 特開2014-093954, 特願2012-245964, ソルビトール誘導性プロモーター

  岩下 和裕; 寺戸 志保; 河内 護之; 豊浦 利枝子

  
• 特許第5846476号, 特開2013-017408, 特願2011-151980, 微生物のコウジ酸生産性を向上させる方法

  岩下 和裕; 河内 護之

  

External funds: Kakenhi

• Mycelial factory for production of biopharmaceuticals using synthetic biology

  Grant-in-Aid for Challenging Research (Pioneering)

  Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields

  Kyoto University

  本田 与一

  From 09 Jul. 2021, To 31 Mar. 2026, Granted

  白色腐朽菌;タンパク質分泌系;タンパク質分泌;ゲノム編集;糖鎖修飾;ゴルジ体

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K18224/
• Development of target-specific bactericidal chimeric phages using a synthetic genome

  Grant-in-Aid for Early-Career Scientists

  Basic Section 38020:Applied microbiology-related

  Kyoto University

  Moriyuki Kawauchi

  From 01 Apr. 2019, To 31 Mar. 2021, Project Closed

  バクテリオファージ;ファージ;人工合成;酵母;CRISPR/Cas13;ファージセラピー;大腸菌

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K15740/
• Modification of the protein secretory system in wood rot fungi

  Grant-in-Aid for Scientific Research (B)

  Basic Section 40020:Wood science-related

  Kyoto University

  Yoichi Honda

  From 01 Apr. 2018, To 31 Mar. 2022, Project Closed

  木材腐朽菌;リグニン分解;タンパク質分泌系;担子菌;分泌系;細胞内小器官;白色腐朽菌;タンパク質分泌;木材分解酵素;遺伝子組換え;リグニン分解酵素;分泌酵素;ゲノム編集;糖鎖修飾

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18H02254/
• 自然環境における放線菌の生理・生態に関する研究

  Grant-in-Aid for JSPS Fellows

  The University of Tokyo

  河内 護之

  From 24 Apr. 2015, To 31 Mar. 2018, Project Closed

  Streptomyces geiseus;autoclaved soil;sporulation;放線菌;土壌培養;Streptomyces griseus;核酸抽出

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15J08354/
• 麹菌のヒストン脱アセチル化酵素遺伝子ＡｏＨｓｔ４の二次代謝制御機構の解析

  Grant-in-Aid for JSPS Fellows

  National Research Institute of Brewing;Hiroshima University

  河内 護之

  From 01 Apr. 2013, To 31 Mar. 2015, Project Closed

  Aohst4;Aospt3;LaeA;二次代謝;分化;SAM;Aspergillus;菌類特異的HDAC;laeA

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13J01633/
• 木材分解酵素の大量生産法を切り拓く腐朽菌の菌糸塊形成機構の解明

  Grant-in-Aid for Scientific Research (C)

  Basic Section 40020:Wood science-related

  Kyoto University

  河内 護之

  From 01 Apr. 2022, To 31 Mar. 2025, Granted

  菌糸塊;木材分解酵素;ヒラタケ;Pleurotus ostreatus;担子菌;酵素生産;高密度培養;細胞壁;腐朽菌

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K05763/
• 糸状菌細胞表層物質の機能解析に基づく基質界面応答の普遍性と特殊性の理解

  Grant-in-Aid for Scientific Research (B)

  Basic Section 38020:Applied microbiology-related

  Kyoto University

  吉見 啓

  From 01 Apr. 2022, To 31 Mar. 2025, Granted

  糸状菌;細胞外マトリクス;分泌タンパク質;細胞表層科学;細胞表層多糖;低分子量分泌タンパク質;固体攻略;菌糸接着

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H02238/
• Isolation of a safe and eco-friendly cultivar in Lentinula edodes by developing new genome editing technologies

  Grant-in-Aid for Scientific Research (A)

  Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields

  Kyoto University

  本田 与一

  From 01 Apr. 2022, To 31 Mar. 2026, Granted

  きのこ;食用担子菌;胞子;遺伝子汚染;ゲノム編集;キノコ;木材腐朽菌

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H00380/
• Genetic pollution caused by edible mushrooms cultivation in east Asia and its technical solution

  Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

  Medium-sized Section 41:Agricultural economics and rural sociology, agricultural engineering, and related fields

  Kyoto University

  本田 与一

  From 07 Oct. 2022, To 31 Mar. 2028, Granted

  食用担子菌;ゲノム編集;遺伝子汚染;胞子形成

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KK0090/
• 糸状菌細胞表層物質の機能解析に基づく基質界面応答の普遍性と特殊性の理解

  Grant-in-Aid for Scientific Research (B)

  Basic Section 38020:Applied microbiology-related

  Kyoto University

  吉見 啓

  From 01 Apr. 2022, To 31 Mar. 2025, Granted

  糸状菌;細胞表層;基質攻略;多糖;SSPs;細胞外マトリクス;分泌タンパク質;細胞表層科学;細胞表層多糖;低分子量分泌タンパク質;固体攻略;菌糸接着

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K23505/
• 環境配慮型バイオマテリアルに適した白色腐朽菌の選抜とその分子育種法の構築

  Fund for the Promotion of Joint International Research (International Collaborative Research)

  Medium-sized Section 38:Agricultural chemistry and related fields

  Kyoto University

  河内 護之

  From 09 Sep. 2024, To 31 Mar. 2029, Adopted

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24KK0119/

External funds: others

• キノコ菌糸の分子育種による環境調和型バイオマテリアルの開発 (2023年度分)

  Japan Society for the Promotion of Science (JSPS), 二国間交流事業共同研究・セミナー

  From 01 Apr. 2023, To 31 Mar. 2024

  代表者

Education

Teaching subject(s)

• From 01 Apr. 2024, To 31 Mar. 2025

  Laboratory Course in Forest Biochemistry

  BG15, Spring, Graduate School of Agriculture, 2.5
• From 01 Apr. 2024, To 31 Mar. 2025

  Laboratory Course in Forest Biochemistry

  BG15, Fall, Graduate School of Agriculture, 2.5
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar in Forest Biochemistry

  BF15, Spring, Graduate School of Agriculture, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar in Forest Biochemistry

  BF15, Fall, Graduate School of Agriculture, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Forest Molecular Biology

  6824, Spring, Faculty of Agriculture, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Forest Biochemistry

  6778, Spring, Faculty of Agriculture, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Laboratory Course in Forest Biochemistry 1

  BC68, Year-long, Graduate School of Agriculture, 5
• From 01 Apr. 2023, To 31 Mar. 2024

  Laboratory Course in Forest Biochemistry 2

  BC69, Year-long, Graduate School of Agriculture, 5
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar in Forest Biochemistry 1

  BB68, Year-long, Graduate School of Agriculture, 4
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar in Forest Biochemistry 2

  BB69, Year-long, Graduate School of Agriculture, 4
• From 01 Apr. 2023, To 31 Mar. 2024

  Forest Molecular Biology

  6824, Spring, Faculty of Agriculture, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Laboratory Course in Forest Biochemistry 2

  BC69, Year-long, Graduate School of Agriculture, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar in Forest Biochemistry 1

  BB68, Year-long, Graduate School of Agriculture, 4
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar in Forest Biochemistry 2

  BB69, Year-long, Graduate School of Agriculture, 4
• From 01 Apr. 2022, To 31 Mar. 2023

  Forest Molecular Biology

  6824, Spring, Faculty of Agriculture, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Laboratory Course in Forest Biochemistry 1

  BC68, Year-long, Graduate School of Agriculture, 5
• From Apr. 2020, To Mar. 2021

  Laboratory Course in Forest Biochemistry 1

  Year-long, 農学研究科
• From Apr. 2020, To Mar. 2021

  Laboratory Course in Forest Biochemistry 2

  Year-long, 農学研究科
• From Apr. 2020, To Mar. 2021

  Seminar in Forest Biochemistry 1

  Year-long, 農学研究科
• From Apr. 2020, To Mar. 2021

  Seminar in Forest Biochemistry2

  Year-long, 農学研究科
• From Apr. 2021, To Mar. 2022

  Laboratory Course in Forest Biochemistry 1

  Year-long, 農学研究科
• From Apr. 2021, To Mar. 2022

  Laboratory Course in Forest Biochemistry 2

  Year-long, 農学研究科
• From Apr. 2021, To Mar. 2022

  Seminar in Forest Biochemistry 1

  Year-long, 農学研究科
• From Apr. 2021, To Mar. 2022

  Seminar in Forest Biochemistry2

  Year-long, 農学研究科