Basic Information

Faculty

• 農学部

Professional Memberships

• 日本応用糖質科学会(旧日本澱粉学会)
• 日本生化学会
• 日本農芸化学会
• The Japanese Society of Applied Glycoscience
• and Agrochemistry
• Biotechnology
• Japan Society for Bioscience
• The Japanese Biochemical Society

Academic Degree

• 農学修士(京都大学)
• 農学博士(京都大学)

Academic Resume (Graduate Schools)

• 京都大学, 大学院農学研究科修士課程食品工学専攻, 修了

Academic Resume (Undergraduate School/Majors)

• 神戸大学, 農学部農芸化学科, 卒業

Research History

• From 1993, To 2005

  京都大学助教授
• From 1993, To 2001

  Kyoto University, Associate Professor
• From 1980, To 1993

  京都大学助手
• From 1980, To 1993

  Kyoto University, Research Assistant

Language of Instruction

• English

ID,URL

• J-Global ID

  200901042900442281

• External link

  http://food.food.kyoto-u.ac.jp

Website(s) (URL(s))

• http://www.structure.kais.kyoto-u.ac.jp/

researchmap URL

• https://researchmap.jp/read0012948

Research

Research Topics, Overview of the research

• Research Topics

  X-Ray crystallography of proteins and enzymes

• Overview of the research

  In order to improve proteins and enzymes important for food industry, we have determined 3D structures of many proteins (Egg white proteins, plant seed proteins, lectins, and so on) and enzymes (amylase, pullulanase, polysaccharide lyase, and so on). The enzyme mechanisms of transglutaminase and protein glutaminase are also investigated by structural analyses of their mature and proenzyme forms.

Research Interests

• 多糖リアーゼ
• アミラーゼ
• 構造生物学

Research Areas

• Life sciences, Food sciences
• Life sciences, Applied biochemistry
• Life sciences, Structural biochemistry

Papers

• Structural and thermodynamic characterization of endo-1,3-β-glucanase: Insights into the substrate recognition mechanism.

  Oda M; Inaba S; Kamiya N; Bekker GJ; Mikami B

  Biochimica et biophysica acta, Mar. 2018, Peer-reviewed

  
• Positive charges on the surface of thaumatin are crucial for the multi-point interaction with the sweet receptor

  Tetsuya Masuda; Satomi Kigo; Mayuko Mitsumoto; Keisuke Ohta; Mamoru Suzuki; Bunzo Mikami; Naofumi Kitabatake; Fumito Tani

  Frontiers in Molecular Biosciences, 13 Feb. 2018, Peer-reviewed

  
• Alternative substrate-bound conformation of bacterial solute-binding protein involved in the import of mammalian host glycosaminoglycans

  Sayoko Oiki; Reiko Kamochi; Bunzo Mikami; Kousaku Murata; Wataru Hashimoto

  SCIENTIFIC REPORTS, Dec. 2017, Peer-reviewed

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• Binding mode of metal ions to the bacterial iron import protein EfeO

  Kanate Temtrirath; Kenji Okumura; Yukie Maruyama; Bunzo Mikami; Kousaku Murata; Wataru Hashimoto

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Nov. 2017, Peer-reviewed

  
• A solute-binding protein in the closed conformation induces ATP hydrolysis in a bacterial ATP-binding cassette transporter involved in the import of alginate

  Ai Kaneko; Kasumi Uenishi; Yukie Maruyama; Nobuhiro Mizuno; Seiki Baba; Takashi Kumasaka; Bunzo Mikami; Kousaku Murata; Wataru Hashimoto

  JOURNAL OF BIOLOGICAL CHEMISTRY, Sep. 2017, Peer-reviewed

  
• A bacterial ABC transporter enables import of mammalian host glycosaminoglycans

  Sayoko Oiki; Bunzo Mikami; Yukie Maruyama; Kousaku Murata; Wataru Hashimoto

  SCIENTIFIC REPORTS, Apr. 2017, Peer-reviewed

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• Atomic resolution structure of serine protease proteinase K at ambient temperature

  Tetsuya Masuda; Mamoru Suzuki; Shigeyuki Inoue; Changyong Song; Takanori Nakane; Eriko Nango; Rie Tanaka; Kensuke Tono; Yasumasa Joti; Takashi Kameshima; Takaki Hatsui; Makina Yabashi; Bunzo Mikami; Osamu Nureki; Keiji Numata; So Iwata; Michihiro Sugahara

  SCIENTIFIC REPORTS, Mar. 2017, Peer-reviewed

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• Role of the Tyr270 residue in 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase from Mesorhizobium loti

  Jun Kobayashi; Hiromi Yoshida; Toshiharu Yagi; Shigehiro Kamitori; Hideyuki Hayashi; Kimihiko Mizutani; Nobuyuki Takahashi; Bunzo Mikami

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, Feb. 2017, Peer-reviewed

  
• Structural and Biochemical Studies Reveal a Putative FtsZ Recognition Site on the Z-ring Stabilizer ZapD

  Hwajung Choi; Kyungjin Min; Bunzo Mikami; Hye-Jin Yoon; Hyung Ho Lee

  MOLECULES AND CELLS, Nov. 2016, Peer-reviewed

  
• "Silent" Amino Acid Residues at Key Subunit Interfaces Regulate the Geometry of Protein Nanocages

  Shengli Zhang; Jiachen Zang; Xiaorong Zhang; Hai Chen; Bunzo Mikami; Guanghua Zhao

  ACS NANO, Nov. 2016, Peer-reviewed

  
• Crystal structure of a putative oligopeptide-binding periplasmic protein from a hyperthermophile

  Hye-Jin Yoon; Hee Jung Kim; Bunzo Mikami; Yeon Gyu Yu; Hyung Ho Lee

  EXTREMOPHILES, Sep. 2016, Peer-reviewed

  
• Conformational Change in the Active Site of Streptococcal Unsaturated Glucuronyl Hydrolase Through Site-Directed Mutagenesis at Asp-115

  Yusuke Nakamichi; Sayoko Oiki; Bunzo Mikami; Kousaku Murata; Wataru Hashimoto

  PROTEIN JOURNAL, Aug. 2016, Peer-reviewed

  
• Structural determinants in bacterial 2-keto-3-deoxy-D-gluconate dehydrogenase KduD for dual-coenzyme specificity

  Ryuichi Takase; Yukie Maruyama; Sayoko Oiki; Bunzo Mikami; Kousaku Murata; Wataru Hashimoto

  PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, Jul. 2016, Peer-reviewed

  
• A Hypersweet Protein: Removal of The Specific Negative Charge at Asp21 Enhances Thaumatin Sweetness

  Tetsuya Masuda; Keisuke Ohta; Naoko Ojiro; Kazuki Murata; Bunzo Mikami; Fumito Tani; Piero Andrea Temussi; Naofumi Kitabatake

  SCIENTIFIC REPORTS, Feb. 2016, Peer-reviewed

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• Crystal structure of the N-myristoylated lipopeptide-bound MHC class I complex

  Daisuke Morita; Yukie Yamamoto; Tatsuaki Mizutani; Takeshi Ishikawa; Juri Suzuki; Tatsuhiko Igarashi; Naoki Mori; Takashi Shiina; Hidetoshi Inoko; Hiroaki Fujita; Kazuhiro Iwai; Yoshimasa Tanaka; Bunzo Mikami; Masahiko Sugita

  NATURE COMMUNICATIONS, Jan. 2016, Peer-reviewed

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• Structure of Spo0M, a sporulation-control protein from Bacillus subtilis

  Yo Sonoda; Kimihiko Mizutani; Bunzo Mikami

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Dec. 2015, Peer-reviewed

  
• Structural and functional studies of a metallo-β-lactamase unveil a new type of structurally encoded nickel-containing heterodinuclear site

  Hwajung Choi; Hee Jung Kim; Atsushi Matsuura; Bunzo Mikami; Hye-Jin Yoon; Hyung Ho Lee

  Acta Crystallographica Section D: Biological Crystallography, 01 Oct. 2015, Peer-reviewed

  
• Preparation and characterization of the RNase H domain of Moloney murine leukemia virus reverse transcriptase

  Kosaku Nishimura; Kanta Yokokawa; Tetsuro Hisayoshi; Kosuke Fukatsu; Ikumi Kuze; Atsushi Konishi; Bunzo Mikami; Kenji Kojima; Kiyoshi Yasukawa

  PROTEIN EXPRESSION AND PURIFICATION, Sep. 2015, Peer-reviewed

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• Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate

  Yukie Maruyama; Takafumi Itoh; Ai Kaneko; Yu Nishitani; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  STRUCTURE, Sep. 2015, Peer-reviewed

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• Metabolic Fate of Unsaturated Glucuronic/Iduronic Acids from Glycosaminoglycans MOLECULAR IDENTIFICATION AND STRUCTURE DETERMINATION OF STREPTOCOCCAL ISOMERASE AND DEHYDROGENASE

  Yukie Maruyama; Sayoko Oiki; Ryuichi Takase; Bunzo Mikami; Kousaku Murata; Wataru Hashimoto

  JOURNAL OF BIOLOGICAL CHEMISTRY, Mar. 2015, Peer-reviewed

  
• Preliminary X-ray analysis of the binding domain of the soybean vacuolar sorting receptor complexed with a sorting determinant of a seed storage protein

  Nobuyuki Maruyama; Tomohiro Goshi; Shigeru Sugiyama; Mayumi Niiyama; Hiroaki Adachi; Kazufumi Takano; Satoshi Murakami; Tsuyoshi Inoue; Yusuke Mori; Hiroyoshi Matsumura; Bunzo Mikami

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Feb. 2015, Peer-reviewed

  
• Crystal structure of class III chitinase from pomegranate provides the insight into its metal storage capacity

  Taro Masuda; Guanghua Zhao; Bunzo Mikami

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Jan. 2015, Peer-reviewed

  
• Crystal structure of 5-Formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase , an NAD(+)-dependent dismutase from Mesorhizobium loti

  Andrew Njagi Mugo; Jun Kobayashi; Bunzo Mikami; Yu Yoshikane; Toshiharu Yagi; Kouhei Ohnishi

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Jan. 2015, Peer-reviewed

  
• Atomic structure of recombinant thaumatin II reveals flexible conformations in two residues critical for sweetness and three consecutive glycine residues

  Tetsuya Masuda; Bunzo Mikami; Fumito Tani

  BIOCHIMIE, Nov. 2014, Peer-reviewed

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• Structure-based Conversion of the Coenzyme Requirement of a Short-chain Dehydrogenase/Reductase Involved in Bacterial Alginate Metabolism

  Ryuichi Takase; Bunzo Mikami; Shigeyuki Kawai; Kousaku Murata; Wataru Hashimoto

  JOURNAL OF BIOLOGICAL CHEMISTRY, Nov. 2014, Peer-reviewed

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• The crystal structure of a crustacean prophenoloxidase provides a clue to understanding the functionality of the type3 copper proteins

  Taro Masuda; Kyosuke Momoji; Takashi Hirata; Bunzo Mikami

  FEBS JOURNAL, Jun. 2014, Peer-reviewed

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• Structure of 4-pyridoxolactonase from Mesorhizobium loti

  Jun Kobayashi; Yu Yoshikane; Toshiharu Yagi; Seiki Baba; Kimihiko Mizutani; Nobuyuki Takahashi; Bunzo Mikami

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Apr. 2014, Peer-reviewed

  
• Crystal Structure of a Bacterial Unsaturated Glucuronyl Hydrolase with Specificity for Heparin*

  Yusuke Nakamichi; Bunzo Mikami; Kousaku Murata; Wataru Hashimoto

  JOURNAL OF BIOLOGICAL CHEMISTRY, Feb. 2014, Peer-reviewed

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• Crystal Structure of Pedobacter heparinus Heparin Lyase Hep III with the Active Site in a Deep Cleft

  Wataru Hashimoto; Yukie Maruyama; Yusuke Nakamichi; Bunzo Mikami; Kousaku Murata

  BIOCHEMISTRY, Feb. 2014, Peer-reviewed

  
• Purification, crystallization and preliminary crystallographic analysis of soybean mature glycinin A1bB2

  Krisna Prak; Bunzo Mikami; Takafumi Itoh; Takako Fukuda; Nobuyuki Maruyama; Shigeru Utsumi

  Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Aug. 2013, Peer-reviewed

  
• Crystal structure of pyridoxine 4-oxidase from Mesorhizobium loti

  Andrew Njagi Mugo; Jun Kobayashi; Taiji Yamasaki; Bunzo Mikami; Kouhei Ohnishi; Yu Yoshikane; Toshiharu Yagi

  BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Jun. 2013, Peer-reviewed

  
• Crystal structure of a major seed storage protein, 11S proglobulin, from Amaranthus hypochondriacus: Insight into its physico-chemical properties

  Mary Rose Tandang-Silvas; Cerrone S. Cabanos; Laura Denisse Carrazco Pena; Ana Paulina Barba De La Rosa; Juan Alberto Osuna-Castro; Shigeru Utsumi; Bunzo Mikami; Nobuyuki Maruyama

  FOOD CHEMISTRY, Nov. 2012, Peer-reviewed

  
• Structure of beta-1,4-mannanase from the common sea hare Aplysia kurodai at 1.05 angstrom resolution

  Kimihiko Mizutani; Sae Tsuchiya; Mayuko Toyoda; Yuko Nanbu; Keiko Tominaga; Keizo Yuasa; Nobuyuki Takahashi; Akihiko Tsuji; Bunzo Mikami

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Oct. 2012, Peer-reviewed

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• Kinetic monitoring of site-directed mutational beta-amylase catalysis on a 27-MHz QCM

  Toshiaki Mori; Masayoshi Shibata; Takanori Nihira; Bunzo Mikami; Yoshio Okahata

  JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, Oct. 2012, Peer-reviewed

  
• Induced-fit motion of a lid loop involved in catalysis in alginate lyase A1-III

  Bunzo Mikami; Mizuho Ban; Sachiko Suzuki; Hye-Jin Yoon; Osamu Miyake; Masayuki Yamasaki; Kohei Ogura; Yukie Maruyama; Wataru Hashimoto; Kousaku Murata

  ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, Sep. 2012, Peer-reviewed

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• Structural and functional characterization of recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris

  Kimihiko Mizutani; Mayuko Toyoda; Yuichiro Otake; Soshi Yoshioka; Nobuyuki Takahashi; Bunzo Mikami

  BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Aug. 2012, Peer-reviewed

  
• The extension peptide of plant ferritin from sea lettuce contributes to shell stability and surface hydrophobicity

  Taro Masuda; Shin-Ichiro Morimoto; Bunzo Mikami; Haruhiko Toyohara

  PROTEIN SCIENCE, Jun. 2012, Peer-reviewed

  
• Recognition of Heteropolysaccharide Alginate by Periplasmic Solute-Binding Proteins of a Bacterial ABC Transporter

  Yu Nishitani; Yukie Maruyama; Takafumi Itoh; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  BIOCHEMISTRY, May 2012, Peer-reviewed

  
• Atomic structure of the sweet-tasting protein thaumatin I at pH 8.0 reveals the large disulfide-rich region in domain II to be sensitive to a pH change

  Tetsuya Masuda; Keisuke Ohta; Bunzo Mikami; Naofumi Kitabatake; Fumito Tani

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Mar. 2012, Peer-reviewed

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• Crystallization and preliminary X-ray analysis of alginate importer from Sphingomonas sp A1

  Yukie Maruyama; Takafumi Itoh; Yu Nishitani; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Mar. 2012, Peer-reviewed

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• X-ray structures of transferrins and related proteins

  Kimihiko Mizutani; Mayuko Toyoda; Bunzo Mikami

  BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, Mar. 2012, Peer-reviewed

  
• Crystallization and preliminary X-ray analysis of pyridoxine 4-oxidase, the first enzyme in pyridoxine degradation pathway I

  Andrew Njagi Mugo; Jun Kobayashi; Bunzo Mikami; Kouhei Ohnishi; Toshiharu Yagi

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Jan. 2012, Peer-reviewed

  
• Crystal Structures of Protein Glutaminase and Its Pro Forms Converted into Enzyme-Substrate Complex

  Ryota Hashizume; Yukiko Maki; Kimihiko Mizutani; Nobuyuki Takahashi; Hiroyuki Matsubara; Akiko Sugita; Kimihiko Sato; Shotaro Yamaguchi; Bunzo Mikami

  JOURNAL OF BIOLOGICAL CHEMISTRY, Nov. 2011, Peer-reviewed

  
• Crystal structure of phosphopantetheine adenylyltransferase from Enterococcus faecalis in the ligand-unbound state and in complex with ATP and pantetheine

  Hye-Jin Yoon; Ji Yong Kang; Bunzo Mikami; Hyung Ho Lee; Se Won Suh

  MOLECULES AND CELLS, Nov. 2011, Peer-reviewed

  
• Crystal structure of the major peanut allergen Ara h 1

  Cerrone Cabanos; Hiroyuki Urabe; Mary Rose Tandang-Silvas; Shigeru Utsumi; Bunzo Mikami; Nobuyuki Maruyama

  MOLECULAR IMMUNOLOGY, Oct. 2011, Peer-reviewed

  
• Formate Oxidase, an Enzyme of the Glucose-Methanol-Choline Oxidoreductase Family, Has a His-Arg Pair and 8-Formyl-FAD at the Catalytic Site

  Daiju Doubayashi; Takumi Ootake; Yosifumi Maeda; Masaya Oki; Yuji Tokunaga; Akihiko Sakurai; Yukio Nagaosa; Bunzo Mikami; Hiroyuki Uchida

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Sep. 2011, Peer-reviewed

  
• Structural Determinants of Discrimination of NAD(+) from NADH in Yeast Mitochondrial NADH Kinase Pos5

  Takuya Ando; Kazuto Ohashi; Akihito Ochiai; Bunzo Mikami; Shigeyuki Kawai; Kousaku Murata

  JOURNAL OF BIOLOGICAL CHEMISTRY, Aug. 2011, Peer-reviewed

  
• Crystal structure of the sweet-tasting protein thaumatin II at 1.27 angstrom

  Tetsuya Masuda; Keisuke Ohta; Fumito Tani; Bunzo Mikami; Naofumi Kitabatake

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Jul. 2011, Peer-reviewed

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• Expression, crystallization and preliminary X-ray diffraction studies of thermostable beta-1,3-xylanase from Thermotoga neapolitana strain DSM 4359

  Fumiyoshi Okazaki; Chiaki Ogino; Akihiko Kondo; Bunzo Mikami; Yoichi Kurebayashi; Hiroki Tsuruta

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Jul. 2011, Peer-reviewed

  
• High-resolution structure of the recombinant sweet-tasting protein thaumatin I

  Tetsuya Masuda; Keisuke Ohta; Bunzo Mikami; Naofumi Kitabatake

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Jun. 2011, Peer-reviewed

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• The Development of Transgenic Crops to Improve Human Health by Advanced Utilization of Seed Storage Proteins

  Nobuyuki Maruyama; Bunzo Mikami; Shigeru Utsumi

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, May 2011, Peer-reviewed

  
• High-throughput construction of expression system using yeast Pichia pastoris, and its application to membrane proteins

  Kimihiko Mizutani; Soshi Yoshioka; Yukiko Mizutani; So Iwata; Bunzo Mikami

  PROTEIN EXPRESSION AND PURIFICATION, May 2011, Peer-reviewed

  
• Heterosubunit Composition and Crystal Structures of a Novel Bacterial M16B Metallopeptidase

  Yukie Maruyama; Asako Chuma; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  JOURNAL OF MOLECULAR BIOLOGY, Mar. 2011, Peer-reviewed

  
• Crystal structure of bacterial cell-surface alginate-binding protein with an M75 peptidase motif

  Yukie Maruyama; Akihito Ochiai; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Feb. 2011, Peer-reviewed

  
• Structural Determinants in Streptococcal Unsaturated Glucuronyl Hydrolase for Recognition of Glycosaminoglycan Sulfate Groups

  Yusuke Nakamichi; Yukie Maruyama; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  JOURNAL OF BIOLOGICAL CHEMISTRY, Feb. 2011, Peer-reviewed

  
• Crystal structure of bacterial cell-surface alginate-binding protein with an M75 peptidase motif

  Yukie Maruyama; Akihito Ochiai; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Feb. 2011, Peer-reviewed

  
• The Crystal Structure of SDR-Type Pyridoxal 4-Dehydrogenase of Mesorhizobium loti

  Huy Nhat Chu; Jun Kobayashi; Bunzo Mikami; Toshiharu Yagi

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Feb. 2011, Peer-reviewed

  
• Single-Crystal X-ray Diffraction Study of a Magnetically Oriented Microcrystal Array of Lysozyme

  Fumiko Kimura; Kimihiko Mizutani; Bunzo Mikami; Tsunehisa Kimurat

  CRYSTAL GROWTH & DESIGN, Jan. 2011, Peer-reviewed

  
• Molecular Design of Seed Storage Proteins for Enhanced Food Physicochemical Properties

  Mary Rose G. Tandang-Silvas; Evelyn Mae Tecson-Mendoza; Bunzo Mikami; Shigeru Utsumi; Nobuyuki Maruyama

  ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 2, 2011, Peer-reviewed

  
• Refolding and Polymerization Pathways of Neuroserpin

  Sayaka Takehara; Juan Zhang; Xiaoyan Yang; Nobuyuki Takahashi; Bunzo Mikami; Maki Onda

  JOURNAL OF MOLECULAR BIOLOGY, Nov. 2010, Peer-reviewed

  
• Functional Analysis of the Cucumisin Propeptide as a Potent Inhibitor of Its Mature Enzyme

  Masataka Nakagawa; Megumi Ueyama; Hiroki Tsuruta; Tomohide Uno; Kengo Kanamaru; Bunzo Mikami; Hiroshi Yamagata

  JOURNAL OF BIOLOGICAL CHEMISTRY, Sep. 2010, Peer-reviewed

  
• The universal mechanism for iron translocation to the ferroxidase site in ferritin, which is mediated by the well conserved transit site

  Taro Masuda; Fumiyuki Goto; Toshihiro Yoshihara; Bunzo Mikami

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Sep. 2010, Peer-reviewed

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• Crystallization and preliminary X-ray analysis of the major peanut allergen Ara h 1 core region

  Cerrone Cabanos; Hiroyuki Urabe; Taro Masuda; Mary Rose Tandang-Silvas; Shigeru Utsumi; Bunzo Mikami; Nobuyuki Maruyama

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Sep. 2010, Peer-reviewed

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• Crystallization and preliminary X-ray analysis of formate oxidase, an enzyme of the glucose-methanol-choline oxidoreductase family

  Yoshifumi Maeda; Daiju Doubayashi; Takumi Ootake; Masaya Oki; Bunzo Mikami; Hiroyuki Uchida

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Sep. 2010, Peer-reviewed

  
• Molecular identification of unsaturated uronate reductase prerequisite for alginate metabolism in Sphingomonas sp A1

  Ryuichi Takase; Akihito Ochiai; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Sep. 2010, Peer-reviewed

  
• Crystal Structure of Exotype Alginate Lyase Atu3025 from Agrobacterium tumefaciens

  Akihito Ochiai; Masayuki Yamasaki; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  JOURNAL OF BIOLOGICAL CHEMISTRY, Aug. 2010, Peer-reviewed

  
• Soybean Basic 7S Globulin: Subunit Heterogeneity and Molecular Evolution

  Andrei D. Shutov; Krisna Park; Takako Fukuda; Sergei V. Rudakov; Angela S. Rudakova; Mary Rose Tandang-Silvas; Keigo Fujiwara; Bunzo Mikami; Shigeru Utsumi; Nobuyuki Maruyama

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Aug. 2010, Peer-reviewed

  
• Mutational studies of the peptidoglycan hydrolase FlgJ of Sphingomonas sp strain A1

  Yukie Maruyama; Akihito Ochiai; Takafumi Itoh; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  JOURNAL OF BASIC MICROBIOLOGY, Aug. 2010, Peer-reviewed

  
• Expression, purification and preliminary crystallization of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.

  Mary Rose Tandang-Silvas; Laura Carrazco-Pena; Ana Paulina Barba de la Rosa; Juan Alberto Osuna-Castro; Shigeru Utsumi; Bunzo Mikami; Nobuyuki Maruyama

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Aug. 2010, Peer-reviewed

  
• Conservation and divergence on plant seed 11S globulins based on crystal structures

  Mary Rose G. Tandang-Silvas; Takako Fukuda; Chisato Fukuda; Krisna Prak; Cerrone Cabanos; Aiko Kimura; Takafumi Itoh; Bunzo Mikami; Shigeru Utsumi; Nobuyuki Maruyama

  BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Jul. 2010, Peer-reviewed

  
• Crystallization and preliminary X-ray analysis of L-azetidine-2-carboxylate hydrolase from Pseudomonas sp strain A2C

  Mayuko Toyoda; Keiji Jitsumori; Bunzo Mikami; Lawrence P. Wackett; Tatsuo Kurihara; Nobuyoshi Esaki

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Jun. 2010, Peer-reviewed

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• X-ray analysis of bilirubin oxidase from Myrothecium verrucaria at 2.3 angstrom resolution using a twinned crystal

  Kimihiko Mizutani; Mayuko Toyoda; Kenta Sagara; Nobuyuki Takahashi; Atsuko Sato; Yuji Kamitaka; Seiya Tsujimura; Yuji Nakanishi; Toshiyuki Sugiura; Shotaro Yamaguchi; Kenji Kano; Bunzo Mikami

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Jun. 2010, Peer-reviewed

  
• Crystallization and preliminary X-ray analysis of SDR-type pyridoxal dehydrogenase from Mesorhizobium loti

  Huy Nhat Chu; Jun Kobayashi; Yu Yoshikane; Bunzo Mikami; Toshiharu Yagi

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Jun. 2010, Peer-reviewed

  
• Crystal Structure of Plant Ferritin Reveals a Novel Metal Binding Site That Functions as a Transit Site for Metal Transfer in Ferritin

  Taro Masuda; Fumiyuki Goto; Toshihiro Yoshihara; Bunzo Mikami

  JOURNAL OF BIOLOGICAL CHEMISTRY, Feb. 2010, Peer-reviewed

  
• Structural and Functional Characterization of Recombinant Human Serum Transferrin Secreted from Pichia pastoris

  Kimihiko Mizutani; Kazuhiko Hashimoto; Nobuyuki Takahashi; Masaaki Hirose; Shigeo Aibara; Bunzo Mikami

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Feb. 2010, Peer-reviewed

  
• Crystal Structure of Cold-Active Alkaline Phosphatase from the Psychrophile Shewanella sp.

  Hiroki Tsuruta; Bunzo Mikami; Tetsuo Higashi; Yasuo Aizono

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Jan. 2010, Peer-reviewed

  
• Protein-Engineering Study of Contribution of Conceivable D-Serine Residues to the Thermostabilization of Ovalbumin under Alkaline Conditions

  Nobuyuki Takahashi; Maki Maeda; Masayuki Yamasaki; Bunzo Mikami

  CHEMISTRY & BIODIVERSITY, 2010, Peer-reviewed

  
• Crystal Structure of Family 14 Polysaccharide Lyase with pH-dependent Modes of Action

  Kohei Ogura; Masayuki Yamasaki; Takashi Yamada; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  JOURNAL OF BIOLOGICAL CHEMISTRY, Dec. 2009, Peer-reviewed

  
• Crystallization and preliminary X-ray analysis of 4-pyridoxolactonase from Mesorhizobium loti

  Sayoko Matsuda; Nana Yokochi; Yu Yoshikane; Jun Kobayashi; Chu Nhat Huy; Seiki Baba; Seiki Kuramitsu; Bunzo Mikami; Toshiharu Yagi

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Sep. 2009, Peer-reviewed

  
• Substrate Specificity of Streptococcal Unsaturated Glucuronyl Hydrolases for Sulfated Glycosaminoglycan

  Yukie Maruyama; Yusuke Nakamichi; Takafumi Itoh; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jul. 2009, Peer-reviewed

  
• Two Crystal Structures of Lysyl-tRNA Synthetase from Bacillus stearothermophilus in Complex with Lysyladenylate-Like Compounds: Insights into the Irreversible Formation of the Enzyme-Bound Adenylate of l-Lysine Hydroxamate

  Haruko Sakurama; Teisuke Takita; Bunzo Mikami; Takafumi Itoh; Kiyoshi Yasukawa; Kuniyo Inouye

  JOURNAL OF BIOCHEMISTRY, May 2009, Peer-reviewed

  
• Crystallization and preliminary crystallographic analysis of the cell-surface alginate-binding protein Algp7 isolated from Sphingomonas sp A1

  Wataru Hashimoto; Akihito Ochiai; Jinshan He; Takafumi Itoh; Bunzo Mikami; Kousaku Murata

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, May 2009, Peer-reviewed

  
• The 2.1-angstrom Crystal Structure of Native Neuroserpin Reveals Unique Structural Elements That Contribute to Conformational Instability

  Sayaka Takehara; Maki Onda; Juan Zhang; Mika Nishiyama; Xiaoyan Yang; Bunzo Mikami; David A. Lomas

  JOURNAL OF MOLECULAR BIOLOGY, Apr. 2009, Peer-reviewed

  
• Structural Determinants Responsible for Substrate Recognition and Mode of Action in Family 11 Polysaccharide Lyases

  Akihito Ochiai; Takafumi Itoh; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  JOURNAL OF BIOLOGICAL CHEMISTRY, Apr. 2009, Peer-reviewed

  
• Crystal structure of the glycosidase family 73 peptidoglycan hydrolase FlgJ

  Wataru Hashimoto; Akihito Ochiai; Keiko Momma; Takafumi Itoh; Bunzo Mikami; Yukie Maruyama; Kousaku Murata

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Mar. 2009, Peer-reviewed

  
• Structure of 3-deoxy-manno-octulosonate cytidylyltransferase from Haemophilus influenzae complexed with the substrate 3-deoxy-manno-octulosonate in the beta-configuration

  Hye-Jin Yoon; Min-Je Ku; Bunzo Mikami; Se Won Suh

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Dec. 2008, Peer-reviewed

  
• The role of group bulkiness in the catalytic activity of psychrophile cold-active protein tyrosine phosphatase

  Hiroki Tsuruta; Bunzo Mikami; Chiaki Yamamoto; Hiroshi Yamagata

  FEBS JOURNAL, Sep. 2008, Peer-reviewed

  
• Substrate recognition by family 7 alginate lyase from Sphingomonas sp A1

  Kohei Ogura; Masayuki Yamasaki; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  JOURNAL OF MOLECULAR BIOLOGY, Jul. 2008, Peer-reviewed

  
• Characterization and crystallography of recombinant 7S globulins of adzuki bean and structure-function relationships with 7S globulins of various crops

  Takako Fukuda; Nobuyuki Maruyama; Mohamad Ramlan Mohamed Salleh; Bunzo Mikami; Shigeru Utsumi

  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, Jun. 2008, Peer-reviewed

  
• Crystal structure of UDP-N-acetylglucosamine enolpyruvyl transferase from Haemophilus influenzae in complex with UDP-N-acetylglucosamine and fosfomycin

  Hye-Jin Yoon; Sang Jae Lee; Bunzo Mikami; Hyun-Ju Park; Jakyung Yoo; Se Won Suh

  PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, May 2008, Peer-reviewed

  
• Crystal structure of YihS in complex with D-mannose: Structural annotation of Escherichia coli and Salmonella enterica yihS-encoded proteins to an aldose-ketose isomerase

  Takafumi Itoh; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  JOURNAL OF MOLECULAR BIOLOGY, Apr. 2008, Peer-reviewed

  
• Crystal structure of a novel bacterial cell-surface flagellin binding to a polysaccharide

  Yukie Maruyama; Misato Momma; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  BIOCHEMISTRY, Feb. 2008, Peer-reviewed

  
• Superchannel of bacteria: Biological significance and new horizons

  Kousaku Murata; Shigeyuki Kawai; Bunzo Mikami; Wataru Hashimoto

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Feb. 2008, Peer-reviewed

  
• Crystal structure of pyridoxamine-pyruvate aminotransferase from Mesorhizobium loti MAFF303099

  Yu Yoshikane; Nana Yokochi; Masayuki Yamasaki; Kimihiko Mizutani; Kouhei Ohnishi; Bunzo Mikami; Hideyuki Hayashi; Toshiharu Yagi

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jan. 2008, Peer-reviewed

  
• A novel structural fold in polysaccharide lyases - Bacillus subtilis family 11 rhamnogalacturonan lyase YesW with an eight-bladed beta-propeller

  Akihito Ochiai; Takafumi Itoh; Yukie Maruyama; Akiko Kawamata; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  JOURNAL OF BIOLOGICAL CHEMISTRY, Dec. 2007, Peer-reviewed

  
• Hydration of vinyl ether groups by unsaturated glycoside hydrolases and their role in bacterial pathogenesis

  Wataru Hashimoto; Takafumi Itoh; Yukie Maruyama; Bunzo Mikami; Kousaku Murata

  INTERNATIONAL MICROBIOLOGY, Dec. 2007, Peer-reviewed

  
• Crystal structure of glycoside hydrolase family 78 alpha-L-rhamnosidase from Bacillus sp GL1

  Zhongli Cui; Yukie Maruyama; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  JOURNAL OF MOLECULAR BIOLOGY, Nov. 2007, Peer-reviewed

  
• Specific and high-affinity binding of tetramerized PD-L1 extracellular domain to PD-1-expressing cells: possible application to enhance T cell function

  Selgo Terawaki; Yoshimasa Tanaka; Tornokazu Nagakura; Tarnon Hayashi; Shiro Shibayama; Kaori Muroi; Taku Okazaki; Bunzo Mikami; David N. Garboczi; Tasuku Honjo; Nagahiro Minato

  INTERNATIONAL IMMUNOLOGY, Jul. 2007, Peer-reviewed

  
• Preliminary crystallographic analysis of L-2-keto-3-deoxyarabonate dehydratase, an enzyme involved in an alternative bacterial pathway of L-arabinose metabolism

  Naoko Shimada; Bunzo Mikami; Seiya Watanabe; Keisuke Makino

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, May 2007, Peer-reviewed

  
• A structural factor responsible for substrate recognition by Bacillus sp GL1 xanthan lyase that acts specifically on pyruvated side chains of Xanthan

  Yukie Maruyama; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  BIOCHEMISTRY, Jan. 2007, Peer-reviewed

  
• Synthesis of pyrophosphate-containing compounds that stimulate V gamma 2V delta 2 T cells: Application to cancer immunotherapy

  Y. Tanaka; H. Kobayashi; T. Terasaki; H. Toma; A. Aruga; T. Uchiyama; K. Mizutani; B. Mikami; C. T. Morita; N. Minato

  MEDICINAL CHEMISTRY, Jan. 2007, Peer-reviewed

  
• Crystal structure of unsaturated glucuronyl hydrolase complexed with substrate

  Takafumi Itoh; Wataru Hashimoto; Bunzo Mikami; Kousaku Murata

  JOURNAL OF BIOLOGICAL CHEMISTRY, Oct. 2006, Peer-reviewed

  
• Structure of unsaturated rhamnogalacturonyl hydrolase complexed with substrate

  Takafumi Toh; Akihito Ochiai; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Sep. 2006, Peer-reviewed

  
• A novel glycoside hydrolase family 105: The structure of family 105 unsaturated rhamnogalacturonyl hydrolase complexed with a disaccharide in comparison with family 88 enzyme complexed with the disaccharide

  Takafumi Itoh; Akihito Ochiai; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  JOURNAL OF MOLECULAR BIOLOGY, Jul. 2006, Peer-reviewed

  
• Crystallization and preliminary crystallographic analysis of the family GH78 alpha-L-rhamnosidase RhaB from Bacillus sp GL1

  ZL Cui; Y Maruyama; B Mikami; W Hashimoto; K Murata

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Jul. 2006, Peer-reviewed

  
• Sequence and analysis of the 46.6-kb plasmid pA1 from Sphingomonas sp A1 that corresponds to the typical IncP-1 beta plasmid backbone without any accessory gene

  KM Harada; Y Aso; W Hashimoto; B Mikami; K Murata

  PLASMID, Jul. 2006, Peer-reviewed

  
• Structure of 8S alpha globulin, the major seed storage protein of mung bean

  T Itoh; RN Garcia; M Adachi; Y Maruyama; EM Tecson-Mendoza; B Mikami; S Utsumi

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Jul. 2006, Peer-reviewed

  
• Crystal structure of pullulanase: Evidence for parallel binding of oligosaccharides in the active site

  Bunzo Mikami; Hiroyuki Iwamoto; Dominggus Malle; Hye-Jin Yoon; Elif Demirkan-Sarikaya; Yoshihiro Mezaki; Yoshio Katsuya

  JOURNAL OF MOLECULAR BIOLOGY, Jun. 2006, Peer-reviewed

  
• Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp GLI

  T Toh; W Hashimoto; B Mikami; K Murata

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, May 2006, Peer-reviewed

  
• Crystallization and preliminary X-ray analysis of an exotype alginate lyase Atu3025 from Agrobacterium tumefaciens strain C58, a member of polysaccharide lyase family 15

  A Ochiai; M Yamasaki; B Mikami; W Hashimoto; K Murata

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, May 2006, Peer-reviewed

  
• Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11

  A Ochiai; M Yamasaki; T Itoh; B Mikami; W Hashimoto; K Murata

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, May 2006, Peer-reviewed

  
• Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168

  D Malle; T Itoh; W Hashimoto; K Murata; S Utsumi; B Mikami

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Apr. 2006, Peer-reviewed

  
• Engineered membrane superchannel improves bioremediation potential of dioxin-degrading bacteria

  Y Aso; Y Miyamoto; KM Harada; K Momma; S Kawai; W Hashimoto; B Mikami; K Murata

  NATURE BIOTECHNOLOGY, Feb. 2006, Peer-reviewed

  
• Crystal structure of a metal ion-bound IS200 transposase

  HH Lee; JY Yoon; HS Kim; JY Kang; KH Kim; DJ Kim; JY Ha; B Mikami; HJ Yoon; SW Suh

  JOURNAL OF BIOLOGICAL CHEMISTRY, Feb. 2006, Peer-reviewed

  
• Crystallization and preliminary X-ray diffraction studies of xylitol dehydrogenase, a key enzyme for bioethanol production using yeast

  J. Matsui; S. Watanabe; T. Kodaki; B. Mikami; K. Makino

  20th IUBMB International Congress of Biochemistry and Molecular Biology，Kyoto Japan，2006.6.18-23, 2006, Peer-reviewed

  
• Crystal structure and some properties of a major house dust mite allergen, Derf 2

  M Suzuki; Y Tanaka; S Korematsu; B Mikami; N Minato

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Jan. 2006, Peer-reviewed

  
• Structure of aluminium-bound ovotransferrin at 2.15 angstrom resolution

  K Mizutani; B Mikami; S Aibara; M Hirose

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Dec. 2005, Peer-reviewed

  
• Proteomics-based identification of outer-membrane proteins responsible for import of macromolecules in Sphingonionas sp A1: Alginate-binding flagellin on the cell surface

  W Hashimoto; JS He; Y Wada; H Nankai; B Mikami; K Murata

  BIOCHEMISTRY, Oct. 2005, Peer-reviewed

  
• A structural basis for depolymerization of alginate by polysaccharide lyase family-7

  M Yamasaki; K Ogura; W Hashimoto; B Mikami; K Murata

  JOURNAL OF MOLECULAR BIOLOGY, Sep. 2005, Peer-reviewed

  
• Glycosylation of genistin into soluble inclusion complex form of cyclic glucans by enzymatic modification

  D Li; SA Roh; JH Shim; B Mikami; MY Baik; CS Park; KH Park

  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, Aug. 2005, Peer-reviewed

  
• Structural basis of a fungal galectin from Agrocybe cylindracea for recognizing sialoconjugate

  M Ban; HJ Yoon; E Demirkan; S Utsumi; B Mikami; F Yagi

  JOURNAL OF MOLECULAR BIOLOGY, Aug. 2005, Peer-reviewed

  
• Crystal structure of nicotinic acid mononucleotide adenylyltransferase from Pseudomonas aeruginosa in its Apo and substrate-complexed forms reveals a fully open conformation

  HJ Yoon; HL Kim; B Mikami; SW Suh

  JOURNAL OF MOLECULAR BIOLOGY, Aug. 2005, Peer-reviewed

  
• Crystal structure of Bacillus sp GL1 xanthan lyase complexed with a substrate: Insights into the enzyme reaction mechanism

  Y Maruyama; W Hashimoto; B Mikami; K Murata

  JOURNAL OF MOLECULAR BIOLOGY, Jul. 2005, Peer-reviewed

  
• alpha-amylase from B-amyloliquefaciens: purification, characterization, raw starch degradation and expression in E-coli

  ES Demirkan; B Mikami; M Adachi; T Higasa; S Utsumi

  PROCESS BIOCHEMISTRY, Jul. 2005, Peer-reviewed

  
• Crystallization and preliminary X-ray crystallographic analysis of UDP-N-Acetylglucosamine enolpyruvyl transferase from Haemophilus influenzae in complex with UDP-N-Acetylglucosamine and fosfomycin

  HJ Yoon; MJ Ku; HJ Ahn; BI Lee; B Mikami; SW Suh

  MOLECULES AND CELLS, Jun. 2005, Peer-reviewed

  
• Molecular conversion of NAD kinase to NADH kinase through single amino acid residue substitution

  S Mori; S Kawai; F Shi; B Mikami; K Murata

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jun. 2005, Peer-reviewed

  
• Direct evidence for Sphingomonas sp A1 periplasmic proteins as macromolecule-binding proteins associated with the ABC transporter: Molecular insights into alginate transport in the periplasm

  K Momma; Y Mishima; W Hashimoto; B Mikami; K Murata

  BIOCHEMISTRY, Apr. 2005, Peer-reviewed

  
• Structure and function of bacterial super-biosystem responsible for import and depolymerization of macromolecules

  W Hashimoto; K Momma; Y Maruyama; M Yamasaki; B Mikami; K Murata

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Apr. 2005, Peer-reviewed

  
• Hypothesis: Structures, evolution, and ancestor of glucose kinases in the hexokinase family

  S Kawai; T Mukai; S Mori; B Mikami; K Murata

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, Apr. 2005, Peer-reviewed

  
• Structural analysis of threonine 342 mutants of soybean-amylase: Role of a conformational change of the inner loop in the catalytic mechanism

  YN Kang; A Tanabe; M Adachi; S Utsumi; B Mikami

  BIOCHEMISTRY, Apr. 2005, Peer-reviewed

  
• Crystallization and preliminary X-ray analysis of alginate lyases A1-II and A1-II ' from Sphingomonas sp A1

  M Yamasaki; K Ogura; S Moriwaki; W Hashimoto; K Murata; B Mikami

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Mar. 2005, Peer-reviewed

  
• NAD-binding mode and the significance of intersubunit contact revealed by the crystal structure of Mycobacterium tuberculosis NAD kinase-NAD complex

  S Mori; M Yamasaki; Y Maruyama; K Momma; S Kawai; W Hashimoto; B Mikami; K Murata

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Feb. 2005, Peer-reviewed

  
• Crystallization and preliminary crystallographic analysis of endo-1,3-beta-glucanase from Arthrobacter sp.

  Z Pang; YN Kang; M Ban; M Oda; R Kobayashi; M Ohnishi; B Mikami

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Jan. 2005, Peer-reviewed

  
• Crystal structure of cold-active protein-tyrosine phosphatase from a psychrophile, Shewanella sp.

  H Tsuruta; B Mikami; Y Aizono

  JOURNAL OF BIOCHEMISTRY, Jan. 2005, Peer-reviewed

  
• Super-channel in bacteria: Structural and functional aspects of a novel biosystem for the import and depolymerization of macromolecules

  W Hashimoto; M Yamasaki; T Itoh; K Momma; B Mikami; K Murata

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, Dec. 2004, Peer-reviewed

  
• Crystallographic studies of Mycobacterium tuberculosis polyphosphate/ATP-NAD kinase complexed with NAD

  S Mori; M Yamasaki; Y Maruyuma; K Momma; S Kawai; W Hashimoto; B Mikami; K Murata

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, Nov. 2004, Peer-reviewed

  
• Crystal structure of bacterial inorganic polyphosphate/ATP-glucomannokinase - Insights into kinase evolution

  T Mukai; S Kawai; S Mori; B Mikami; K Murata

  JOURNAL OF BIOLOGICAL CHEMISTRY, Nov. 2004, Peer-reviewed

  
• Crystal structure of peptide deformylase from Staphylococcus aureus in complex with actinonin, a naturally occurring antibacterial agent

  HJ Yoon; HL Kim; SK Lee; HW Kim; HW Kim; JY Lee; B Mikami; SW Suh

  PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, Nov. 2004, Peer-reviewed

  
• Crystallization and preliminary X-ray analysis of pyridoxal 4-dehydrogenase, the second enzyme in degradation pathway I of pyridoxine

  N Yokochi; Y Yoshikane; T Yagi; M Yamasaki; B Mikami

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Nov. 2004, Peer-reviewed

  
• Engineering of the pH optimum of Bacillus cereus beta-amylase: Conversion of the pH optimum from a bacterial type to a higher-plant type

  A Hirata; M Adachi; S Utsumi; B Mikami

  BIOCHEMISTRY, Oct. 2004, Peer-reviewed

  
• Crystallization and preliminary crystallographic analysis of monodehydroascorbate radical reductase from cucumber

  S Sano; YN Kang; H Shigemizu; N Morishita; HJ Yoon; K Saito; K Asada; B Mikami

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Aug. 2004, Peer-reviewed

  
• Structure and function of a hypothetical Pseudomonas aeruginosa protein PA1167 classified into family PL-7 - A novel alginate lyase with a beta-sandwich fold

  M Yamasaki; S Moriwaki; O Miyake; W Hashimoto; K Murata; B Mikami

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jul. 2004, Peer-reviewed

  
• Crystal structure of unsaturated glucuronyl hydrolase, responsible for the degradation of glycosaminoglycan, from Bacillus sp GL1 at 1.8 angstrom resolution

  T Itoh; S Akao; W Hashimoto; B Mikami; K Murata

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jul. 2004, Peer-reviewed

  
• The roles of Glu186 and Glu380 in the catalytic reaction of soybean beta-amylase

  YN Kang; M Adachi; S Utsumi; B Mikami

  JOURNAL OF MOLECULAR BIOLOGY, Jun. 2004, Peer-reviewed

  
• A redox-controlled molecular switch revealed by the crystal structure of a bacterial heme PAS sensor

  H Kurokawa; DS Lee; M Watanabe; Sagami, I; B Mikami; CS Raman; T Shimizu

  JOURNAL OF BIOLOGICAL CHEMISTRY, May 2004, Peer-reviewed

  
• Crystallization and preliminary X-ray crystallographic analysis of nicotinic acid mononucleotide adenylyltransferase from Pseudomonas aeruginosa

  HL Kim; HJ Yoon; JY Ha; BI Lee; HH Lee; B Mikami; SW Suh

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, May 2004, Peer-reviewed

  
• Posttranslational processing of polysaccharide lyase: maturation route for gellan lyase in Bacillus sp GL1

  O Miyake; E Kobayashi; H Nankai; W Hashimoto; B Mikami; K Murata

  ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, Feb. 2004, Peer-reviewed

  
• Structure of the core region of the soybean beta-conglycinin alpha ' subunit

  Y Maruyama; N Maruyama; B Mikami; S Utsumi

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Feb. 2004, Peer-reviewed

  
• Structural and enzymatic analysis of soybean beta-amylase mutants with increased pH optimum

  A Hirata; M Adachi; A Sekine; YN Kang; S Utsumi; B Mikami

  JOURNAL OF BIOLOGICAL CHEMISTRY, Feb. 2004, Peer-reviewed

  
• Change in the crystal packing of soybean beta-amylase mutants substituted at a few surface amino acid residues

  YN Kang; M Adachi; B Mikami; S Utsumi

  PROTEIN ENGINEERING, Nov. 2003, Peer-reviewed

  
• Recognition mechanism of non-peptide antigens by human gamma delta T cells

  S Yamashita; Y Tanaka; M Harazaki; B Mikami; N Minato

  INTERNATIONAL IMMUNOLOGY, Nov. 2003, Peer-reviewed

  
• X-ray crystallographic studies of HemK from Thermotoga maritima, an N-5-glutamine methyltransferase

  HJ Yoon; KY Kang; HJ Ahn; SM Shim; JY Ha; SK Lee; B Mikami; SW Suh

  MOLECULES AND CELLS, Oct. 2003, Peer-reviewed

  
• Crystallization and preliminary X-ray analysis of inorganic poly phosphate/ATP-glucomannokinase from Arthrobacter sp strain KM

  T Mukai; S Kawai; S Mori; B Mikami; K Murata

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Sep. 2003, Peer-reviewed

  
• Crystallization and preliminary X-ray analysis of alginate lyase, a member of family PL-7, from Pseudomonas aeruginosa

  M Yamasaki; S Moriwaki; W Hashimoto; B Mikami; K Murata

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Aug. 2003, Peer-reviewed

  
• A novel member of glycoside hydrolase family 88: overexpression, purification, and characterization of unsaturated beta-glucuronyl hydrolase of Bacillus sp GL1

  S Mori; S Akao; H Nankai; W Hashimoto; B Mikami; K Murata

  PROTEIN EXPRESSION AND PURIFICATION, May 2003, Peer-reviewed

  
• Crystallization and preliminary X-ray analysis of a novel unsaturated glucuronyl hydrolase from Bacillus sp GL1

  S Mori; S Akao; O Miyake; H Nankai; W Hashimoto; B Mikami; K Murata

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, May 2003, Peer-reviewed

  
• Crystal structure of Bacillus sp GL1 xanthan lyase, which acts on the side chains of xanthan

  W Hashimoto; H Nankai; B Mikami; K Murata

  JOURNAL OF BIOLOGICAL CHEMISTRY, Feb. 2003, Peer-reviewed

  
• Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp A1, complexed with an alginate tetrasaccharide at 1.6-A resolution

  Y Mishima; K Momma; W Hashimoto; B Mikami; K Murata

  JOURNAL OF BIOLOGICAL CHEMISTRY, Feb. 2003, Peer-reviewed

  
• Crystallization and preliminary X-ray crystallographic analysis of plant ferritin from Glycine max

  T Masuda; B Mikami; F Goto; T Yoshihara; S Utsumi

  BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Jan. 2003, Peer-reviewed

  
• Crystallization and preliminary X-ray studies of cold-active protein-tyrosine phosphatase of Shewanella sp.

  H Tsuruta; B Mikami; C Yamamoto; Y Aizono

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Sep. 2002, Peer-reviewed

  
• Structure and function of bacterial "Super-channel" for macromolecule import

  W Hashimoto; B Mikami; K Murata

  SEIKAGAKU, Jul. 2002, Peer-reviewed

  
• Reaction mechanism of alanine racemase from Bacillus stearothermophilus - X-ray crystallographic studies of the enzyme bound with N-(5 '-phosphopyridoxyl)alanine

  A Watanabe; T Yoshimura; B Mikami; H Hayashi; H Kagamiyama; N Esaki

  JOURNAL OF BIOLOGICAL CHEMISTRY, May 2002, Peer-reviewed

  
• Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp A1 at 2.0 angstrom resolution

  K Momma; B Mikami; Y Mishima; W Hashimoto; K Murata

  JOURNAL OF MOLECULAR BIOLOGY, Mar. 2002, Peer-reviewed

  
• Loop-inserted and theromstabilized structure of P1-P1 ' cleaved ovalbumin mutant R339T

  M Yamasaki; Y Arii; B Mikami; M Hirose

  JOURNAL OF MOLECULAR BIOLOGY, Jan. 2002, Peer-reviewed

  
• A super-channel in bacteria: Macromolecule uptake and depolymerization systems of Sphingomonas sp A1 with a special cell surface structure

  Y Mishima; K Momma; O Miyake; W Hashimoto; B Mikami; K Murata

  BIOTECHNOLOGY & GENETIC ENGINEERING REVIEWS, VOL 19, 2002, Peer-reviewed

  
• Essential contribution of germline-encoded lysine residues in J gamma 1.2 segment to the recognition of nonpeptide antigens by human gamma delta T cells

  F Miyagawa; Y Tanaka; S Yamashita; B Mikami; K Danno; M Uehara; N Minato

  JOURNAL OF IMMUNOLOGY, Dec. 2001, Peer-reviewed

  
• Super-channel in bacteria: function and structure of the macromolecule import system mediated by a pit-dependent ABC transporter

  Y Mishima; K Momma; W Hashimoto; B Mikami; K Murata

  FEMS MICROBIOLOGY LETTERS, Nov. 2001, Peer-reviewed

  
• Purification and crystallization of alpha-amylases from mucoid and non-mucoid B-amyloliquefaciens strains

  E Sarikaya; B Mikami

  JOURNAL OF CRYSTAL GROWTH, Nov. 2001, Peer-reviewed

  
• Super-channel in bacteria: Function and structure of a macromolecule import system mediated by a pit-dependent ABC transporter

  W Hashimoto; K Momma; Y Mishima; B Mikami; K Murata

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Sep. 2001, Peer-reviewed

  
• Crystallization and preliminary X-ray analysis of NAD kinase from Mycobacterium tuberculosis H37Rv

  S Mori; S Kawai; B Mikami; K Murata

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Sep. 2001, Peer-reviewed

  
• Anion-mediated Fe3+ release mechanism in ovotransferrin C-lobe - A structurally identified SO42- binding site and its implications for the kinetic pathway

  K Mizutani; BK Muralidhara; H Yamashita; S Tabata; B Mikami; M Hirose

  JOURNAL OF BIOLOGICAL CHEMISTRY, Sep. 2001, Peer-reviewed

  
• Domain closure mechanism in transferrins: New viewpoints about the hinge structure and motion as deduced from high resolution crystal structures of ovotransferrin N-lobe

  K Mizutani; B Mikami; M Hirose

  JOURNAL OF MOLECULAR BIOLOGY, Jun. 2001, Peer-reviewed

  
• Crystallization and preliminary X-ray analysis of AlgS, a bacterial ATP-binding cassette (ABC) protein specific to macromolecule import

  Y Mishima; K Momma; W Hashimoto; B Mikami; K Murata

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Jun. 2001, Peer-reviewed

  
• Crystal structures of recombinant and native soybean beta-conglycinin beta homotrimers

  N Maruyama; M Adachi; K Takahashi; K Yagasaki; M Kohno; Y Takenaka; E Okuda; S Nakagawa; B Mikami; S Utsumi

  EUROPEAN JOURNAL OF BIOCHEMISTRY, Jun. 2001, Peer-reviewed

  
• Effect of His192 mutation on the activity of alginate lyase A1-III from Sphingomonas species A1

  HJ Yoon; YJ Choi; O Miyake; W Hashimoto; K Murata; B Mikami

  JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, Feb. 2001, Peer-reviewed

  
• Crystal structure of soybean proglycinin alaB1b homotrimer

  M Adachi; Y Takenaka; AB Gidamis; B Mikami; S Utsumi

  JOURNAL OF MOLECULAR BIOLOGY, Jan. 2001, Peer-reviewed

  
• Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 Å resolution

  H.-J. Yoon; W. Hashimoto; O. Miyake; K. Murata; B. Mikami

  Journal of Molecular Biology, 2001, Peer-reviewed

  
• Crystal structure of N-acyl-D-glucosamine 2-epimerase from porcine kidney at 2.0 Å resolution

  T. Itoh; B. Mikami; I. Maru; Y. Ohta; W. Hashimoto; K. Murata

  Journal of Molecular Biology, 10 Nov. 2000, Peer-reviewed

  
• C8/119S mutation of major mite allergen Derf-2 leads to degenerate secondary structure and molecular polymerization and induces potent and exclusive Th1 cell differentiation

  S Korematsu; Y Tanaka; S Hosoi; S Koyanagi; T Yokota; B Mikami; N Minato

  JOURNAL OF IMMUNOLOGY, Sep. 2000, Peer-reviewed

  
• Crystallization and preliminary X-ray crystallographic analysis of NADPH: azodicarbonyl/quinone oxidoreductase, a plant zeta-crystallin

  J Mano; HJ Yoon; K Asada; E Babiychuk; D Inze; B Mikami

  BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, Jul. 2000, Peer-reviewed

  
• Overexpression in Escherichia coli, purification, and characterization of Sphingomonas sp A1 alginate lyases

  HJ Yoon; W Hashimoto; O Miyake; M Okamoto; B Mikami; K Murata

  PROTEIN EXPRESSION AND PURIFICATION, Jun. 2000, Peer-reviewed

  
• Crystal structure at 1.9 Å Resolution of the apoovotransferrin N-lobe bound by sulfate anions: Implications for the domain opening and iron release mechanism

  K. Mizutani; H. Yamashita; B. Mikami; M. Hirose

  Biochemistry, 28 Mar. 2000, Peer-reviewed

  
• Crystallization and preliminary X-ray crystallographic analysis of alginate lyase A1-II from Sphingomonas species A1

  H.-J. Yoon; W. Hashimoto; Y. Katsuya; Y. Mezaki; K. Murata; B. Mikami

  Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology, 09 Feb. 2000, Peer-reviewed

  
• Comparison of degradation abilities of alpha- and beta-amylases on raw starch granules

  E Sarikaya; T Higasa; M Adachi; B Mikami

  PROCESS BIOCHEMISTRY, Feb. 2000, Peer-reviewed

  
• Crystal structure at 1.9 (]J1009[) resolution of apo-ovotransferrin N-lobe bound by sulfate anions : Implication for the domain opening and iron release

  三上 文三

  Biochemistry,39/3258,, 2000, Peer-reviewed

  
• Crystal structure of hen apo-ovotransferrin - Both lopes adopt an open conformation upon loss of iron

  H Kurokawa; JC Dewan; B Mikami; JC Sacchettini; M Hirose

  JOURNAL OF BIOLOGICAL CHEMISTRY, Oct. 1999, Peer-reviewed

  
• Tyrosine 265 of alanine racemase serves as a base abstracting alpha-hydrogen from L-alanine: The counterpart residue to lysine 39 specific to D-alanine

  A Watanabe; T Yoshimura; B Mikami; N Esaki

  JOURNAL OF BIOCHEMISTRY, Oct. 1999, Peer-reviewed

  
• Special cell surface structure, and novel macromolecule transport/depolymerization system of Sphingomonas sp A1

  K Momma; W Hashimoto; O Miyake; HJ Yoon; S Kawai; Y Mishima; B Mikami; K Murata

  JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, Oct. 1999, Peer-reviewed

  
• Structure of the starch-binding domain of Bacillus cereus beta-amylase

  HJ Yoon; A Hirata; M Adachi; A Sekine; S Utsumi; B Mikami

  JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, Oct. 1999, Peer-reviewed

  
• Crystal structure of alginate lyase A1-III from Sphingomonas species A1 at 1.78 Å resolution

  H.-J. Yoon; B. Mikami; W. Hashimoto; K. Murata

  Journal of Molecular Biology, 09 Jul. 1999, Peer-reviewed

  
• Structure of raw starch-digesting Bacillus cereus beta-amylase complexed with maltose

  B Mikami; M Adachi; T Kage; E Sarikaya; T Nanmori; R Shinke; S Utsumi

  BIOCHEMISTRY, Jun. 1999, Peer-reviewed

  
• Structure of raw starch-digesting Bacillus cereus beta-amylase complexed with maltose

  B Mikami; M Adachi; T Kage; E Sarikaya; T Nanmori; R Shinke; S Utsumi

  BIOCHEMISTRY, Jun. 1999, Peer-reviewed

  
• Alternative structural state of transferrin - The crystallographic analysis of iron-loaded but domain-opened ovotransferrin N-lobe

  K Mizutani; H Yamashita; H Kurokawa; B Mikami; M Hirose

  JOURNAL OF BIOLOGICAL CHEMISTRY, Apr. 1999, Peer-reviewed

  
• Enzymatic and genetic bases on assimilation, depolymerization, and transport of heteropolysaccharides in bacteria

  W Hashimoto; K Momma; H Miki; Y Mishima; E Kobayashi; O Miyake; S Kawai; H Nankai; B Mikami; K Murata

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, Feb. 1999, Peer-reviewed

  
• Crystal structure of alginate lyase A1-(]G0003[) from Sphingomanas Species A1 at 1.78 A resolution.

  三上 文三

  Journal of Molecular Biology,290/, 1999, Peer-reviewed

  
• Purification and some properties of pheophorbidase in Chenopodium album

  K Watanabe; H Ohta; T Tsuchiya; B Mikami; T Masuda; Y Shioi; K Takamiya

  PLANT AND CELL PHYSIOLOGY, Jan. 1999, Peer-reviewed

  
• Structure of separated starch-binding domain of ┣DBBacillus cereus(/)-┫DB β-amylase

  三上 文三

  The Journal of Microbiology and Biotechnology,9/619,, 1999, Peer-reviewed

  
• Alternative structual state of transterrin

  三上 文三

  Journal of Biological Chemistry,274/, 1999, Peer-reviewed

  
• The crystal structure of the sevenfold mutant of barley β-amylase with increased thermostability at 2.5 Å resolution

  B. Mikami; H.-J. Yoon; N. Yoshigi

  Journal of Molecular Biology, 1999, Peer-reviewed

  
• Crystal structure of recombinant soybean beta-amylase complexed with beta-cyclodextrin

  M Adachi; B Mikami; T Katsube; S Utsumi

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jul. 1998, Peer-reviewed

  
• Purification and characterization of two isozymes of chlorophyllase from mature leaves of Chenopodium album

  T Tsuchiya; H Ohta; T Masuda; B Mikami; N Kita; Y Shioi; K Takamiya

  PLANT AND CELL PHYSIOLOGY, Sep. 1997, Peer-reviewed

  
• β-アミラーゼの構造を見る

  三上 文三

  化学と生物,35/3,199, 1997, Peer-reviewed

  
• The crystal structure of lipase II from Rhizopus niveus at 2.2 Å resolution

  M. Kohno; J. Funatsu; B. Mikami; W. Kugimiya; T. Matsuo; Y. Morita

  Journal of Biochemistry, Sep. 1996, Peer-reviewed

  
• Crystallization and preliminary X-ray diffraction studies of N-acyl-D-glucosamine 2-epimerase from porcine kidney

  Maru, I; J Ohnishi; Y Ohta; W Hashimoto; Y Tsukada; K Murata; B Mikami

  JOURNAL OF BIOCHEMISTRY, Sep. 1996, Peer-reviewed

  
• Crystallization and X-ray Analysis of Normal and Modified Recombinant Soybean Proglycinins: Three-Dimensional Structure of Normal Proglycinin at 6 Å Resolution

  S. Utsumi; A.B. Gidamis; Y. Takenaka; N. Maruyama; M. Adachi; B. Mikami

  ACS Symposium Series, 1996, Peer-reviewed

  
• The crystal structure of lipase II from ┣DBRhizopus(/)-┫DB ┣DBniveus(/)-┫DB at 2.2 (]J1009[) resolution

  三上 文三

  The Journal of Biochemistry,120/,505-510, 1996, Peer-reviewed

  
• THE ROLE OF SH AND S-S GROUPS IN BACILLUS-CEREUS BETA-AMYLASE

  K NOMURA; YONEDA, I; T NANMORI; R SHINKE; Y MORITA; B MIKAMI

  JOURNAL OF BIOCHEMISTRY, Dec. 1995, Peer-reviewed

  
• Crystal structure of diferric hen ovotransferrin at 2.4 Å resolution

  H. Kurokawa; B. Mikami; M. Hirose

  Journal of Molecular Biology, 24 Nov. 1995, Peer-reviewed

  
• MOLECULAR CHARACTERIZATION OF MONODEHYDROASCORBATE RADICAL REDUCTASE FROM CUCUMBER HIGHLY EXPRESSED IN ESCHERICHIA-COLI

  S SANO; C MIYAKE; B MIKAMI; K ASADA

  JOURNAL OF BIOLOGICAL CHEMISTRY, Sep. 1995, Peer-reviewed

  
• β-アミラーゼの活性中心

  三上 文三

  日本農藝化學會誌, 01 Aug. 1995, Peer-reviewed

  
• Active site of soybeanβ-amylase

  三上 文三

  ,69/8,1046-1049, 1995, Peer-reviewed

  
• CRYSTAL-STRUCTURES OF SOYBEAN BETA-AMYLASE REACTED WITH BETA-MALTOSE AND MALTAL - ACTIVE-SITE COMPONENTS AND THEIR APPARENT ROLES IN CATALYSTS

  B MIKAMI; M DEGANO; EJ HEHRE; JC SACCHETTINI

  BIOCHEMISTRY, Jun. 1994, Peer-reviewed

  
• CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF COPPER AMINE OXIDASE FROM ESCHERICHIA-COLI K-12

  JH ROB; H SUZUKI; H KUMAGAI; M YAMASHITA; H AZAKAMI; Y MUROOKA; B MIKAMI

  JOURNAL OF MOLECULAR BIOLOGY, May 1994, Peer-reviewed

  
• CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF SOYBEAN PROGLYCININS MODIFIED BY PROTEIN ENGINEERING

  AB GIDAMIS; B MIKAMI; T KATSUBE; S UTSUMI; M KITO

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Apr. 1994, Peer-reviewed

  
• CRUCIAL ROLE OF INTRALOBE PEPTIDE-PEPTIDE INTERACTIONS IN THE UPTAKE AND RELEASE OF IRON BY OVOTRANSFERRIN

  H KUROKAWA; B MIKAMI; M HIROSE

  JOURNAL OF BIOLOGICAL CHEMISTRY, Mar. 1994, Peer-reviewed

  
• Two sulfhydryl groups near the active site of soybean beta-amylase

  B. Mikami; K. Nomura; Y. Morita

  Bioscience, Biotechnology and Biochemistry, Jan. 1994, Peer-reviewed

  
• CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF BACTERIAL ALGINATE LYASE

  B MIKAMI; T HISANO; T YAMASHITA; T INOSE; M TAKAGI; K SAKAGUCHI; A KIMURA; T IMANAKA; K MURATA

  JOURNAL OF FERMENTATION AND BIOENGINEERING, 1994, Peer-reviewed

  
• STRUCTURAL EVIDENCE FOR A PH-SENSITIVE DILYSINE TRIGGER IN THE HEN OVOTRANSFERRIN N-LOBE - IMPLICATIONS FOR TRANSFERRIN IRON RELEASE

  JC DEWAN; B MIKAMI; M HIROSE; JC SACCHETTINI

  BIOCHEMISTRY, Nov. 1993, Peer-reviewed

  
• CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC ANALYSIS OF THE SOYBEAN PROGLYCININ EXPRESSED IN ESCHERICHIA-COLI

  S UTSUMI; AB GIDAMIS; B MIKAMI; M KITO

  JOURNAL OF MOLECULAR BIOLOGY, Sep. 1993, Peer-reviewed

  
• CLONING OF THE BETA-AMYLASE GENE FROM BACILLUS-CEREUS AND CHARACTERISTICS OF THE PRIMARY STRUCTURE OF THE ENZYME

  T NANMORI; M NAGAI; Y SHIMIZU; R SHINKE; B MIKAMI

  APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb. 1993, Peer-reviewed

  
• The 2.0-Å resolution structure of soybean β-amylase complexed with α-cyclodextrin

  B. Mikami; E.J. Hehre; M. Sato; Y. Katsube; M. Hirose; Y. Morita; J.C. Sacchettini

  Biochemistry, 1993, Peer-reviewed

  
• オボトランスフェリンのX線結晶構造解析

  三上 文三

  京都大学食糧科学研究所報告,56,27-28, 1993, Peer-reviewed

  
• Three-dimensional structure of soybean β-amylase determined at 3.0 Å resolution: Preliminary chain tracing of the complex with α-cyclodextrin

  B. Mikami; M. Sato; T. Shibata; M. Hirose; S. Aibara; Y. Katsube; Y. Morita

  Journal of Biochemistry, 1992, Peer-reviewed

  
• COMPLETE AMINO-ACID-SEQUENCE OF ENDO-BETA-N-ACETYLGLUCOSAMINIDASE FROM FLAVOBACTERIUM SP

  K TAKEGAWA; B MIKAMI; S IWAHARA; Y MORITA; K YAMAMOTO; T TOCHIKURA

  EUROPEAN JOURNAL OF BIOCHEMISTRY, Nov. 1991, Peer-reviewed

  
• X-ray Crystal Structure Analysis of Soybean .BETA.-Amylase.

  MIKAMI Bunzo; SHIBATA Takumi; HIROSE Masaaki; AIBARA Shigeo; SATO Mamoru; KATSUBE Yukiteru; MORITA Yuhei

  Journal of Applied Glycoscience, 1991, Peer-reviewed

  
• X-ray crytal structure analysis of soybean β-amylase

  三上 文三

  Denpun Kagaku,38/2, 1991, Peer-reviewed

  
• CRYSTALLIZATION OF AND PRELIMINARY CRYSTALLOGRAPHIC DATA FOR THE N-TERMINAL HALF-MOLECULE OF OVOTRANSFERRIN

  B MIKAMI; M HIROSE

  JOURNAL OF BIOCHEMISTRY, Dec. 1990, Peer-reviewed

  
• POSITIONS OF SUBSTITUTED AMINO-ACIDS IN SOYBEAN BETA-AMYLASE ISOZYMES

  ES JI; B MIKAMI; JP KIM; Y MORITA

  AGRICULTURAL AND BIOLOGICAL CHEMISTRY, Nov. 1990, Peer-reviewed

  
• CRYSTALLIZATION OF AND PRELIMINARY CRYSTALLOGRAPHIC DATA FOR ALLOSTERIC L-LACTATE DEHYDROGENASE FROM BIFIDOBACTERIUM-LONGUM

  S IWATA; T MINOWA; B MIKAMI; Y MORITA; T OHTA

  JOURNAL OF BIOCHEMISTRY, Oct. 1989, Peer-reviewed

  
• ダイズβ‐アミラーゼの構造とＳＨ基の反応性

  三上文三; 野村啓一; 馬島肇一; 森田雄平

  澱粉科学, Jun. 1989, Peer-reviewed

  
• SPINACH FERREDOXIN-NITRITE REDUCTASE - CHARACTERIZATION OF CATALYTIC ACTIVITY AND INTERACTION OF THE ENZYME WITH SUBSTRATES

  B MIKAMI; S IDA

  JOURNAL OF BIOCHEMISTRY, Jan. 1989, Peer-reviewed

  
• PRIMARY STRUCTURE OF AN N-LINKED SUGAR CHAIN DERIVED FROM GLUCOAMYLASE OF RHIZOPUS-NIVEUS

  K TAKEGAWA; N KAWASAKI; S IWAHARA; K YAMAMOTO; T TOCHIKURA; B MIKAMI; Y MORITA

  BIOCHIMICA ET BIOPHYSICA ACTA, Jan. 1989, Peer-reviewed

  
• Structure of soybean .BETA.-amylase and the reactivity of its sulfhydryl groups.

  MIKAMI Bunzo; NOMURA Keiichi; MAJIMA Keiichi; MORITA Yuhei

  Journal of Applied Glycoscience, 1989, Peer-reviewed

  
• CRYSTALLIZATION OF AND PRELIMINARY CRYSTALLOGRAPHIC DATA FOR BACILLUS-STEAROTHERMOPHILUS CYCLODEXTRIN GLUCANOTRANSFERASE

  M KUBOTA; B MIKAMI; Y TSUJISAKA; Y MORITA

  JOURNAL OF BIOCHEMISTRY, Jul. 1988, Peer-reviewed

  
• ELUCIDATION OF THE ROLE OF SUGAR CHAINS IN GLUCOAMYLASE USING ENDO-BETA-N-ACETYLGLUCOSAMINIDASE FROM FLAVOBACTERIUM SP

  K TAKEGAWA; M INAMI; K YAMAMOTO; H KUMAGAI; T TOCHIKURA; B MIKAMI; Y MORITA

  BIOCHIMICA ET BIOPHYSICA ACTA, Jul. 1988, Peer-reviewed

  
• PURIFICATION, CRYSTALLIZATION, AND CHARACTERIZATION OF PEROXIDASE FROM COPRINUS-CINEREUS

  Y MORITA; H YAMASHITA; B MIKAMI; H IWAMOTO; S AIBARA; M TERADA; J MINAMI

  JOURNAL OF BIOCHEMISTRY, Apr. 1988, Peer-reviewed

  
• PRIMARY STRUCTURE AND FUNCTION OF BETA-AMYLASE

  B MIKAMI; Y MORITA; C FUKAZAWA

  SEIKAGAKU, Mar. 1988, Peer-reviewed

  
• Primary structure and function of beta-amylase

  B. Mikami; Y. Morita; C. Fukazawa

  Seikagaku. The Journal of Japanese Biochemical Society, Mar. 1988, Peer-reviewed

  
• PARTIAL AMINO-ACID-SEQUENCES AROUND SULFHYDRYL-GROUPS OF SOYBEAN BETA-AMYLASE

  K NOMURA; B MIKAMI; Y MORITA

  JOURNAL OF BIOCHEMISTRY, Aug. 1987, Peer-reviewed

  
• EFFECT OF MODIFICATION OF SULFHYDRYL-GROUPS IN SOYBEAN BETA-AMYLASE ON THE INTERACTION WITH SUBSTRATE AND INHIBITORS

  K NOMURA; B MIKAMI; Y NAGAO; Y MORITA

  JOURNAL OF BIOCHEMISTRY, Aug. 1987, Peer-reviewed

  
• INTERACTION OF SOYBEAN BETA-AMYLASE WITH GLUCOSE

  K NOMURA; B MIKAMI; Y MORITA

  JOURNAL OF BIOCHEMISTRY, Nov. 1986, Peer-reviewed

  
• REVERSIBLE INACTIVATION OF FERREDOXIN-NITRATE REDUCTASE FROM THE CYANOBACTERIUM PLECTONEMA-BORYANUM - THE ROLE OF SUPEROXIDE ANION AND CYANIDE

  B MIKAMI; S IDA

  PLANT AND CELL PHYSIOLOGY, Sep. 1986, Peer-reviewed

  
• N-TERMINAL SEQUENCE OF SOYBEAN BETA-AMYLASE

  B MIKAMI; K NOMURA; Y MORITA

  JOURNAL OF BIOCHEMISTRY, Aug. 1986, Peer-reviewed

  
• CHANGES IN LIPOXYGENASE COMPONENTS OF RICE SEEDLINGS DURING GERMINATION

  H OHTA; S IDA; B MIKAMI; Y MORITA

  PLANT AND CELL PHYSIOLOGY, Jul. 1986, Peer-reviewed

  
• SPINACH FERREDOXIN NITRITE REDUCTASE - A PURIFICATION PROCEDURE AND CHARACTERIZATION OF CHEMICAL-PROPERTIES

  S IDA; B MIKAMI

  BIOCHIMICA ET BIOPHYSICA ACTA, Jun. 1986, Peer-reviewed

  
• PURIFICATION AND PROPERTIES OF FERREDOXIN NITRATE REDUCTASE FROM THE CYANOBACTERIUM PLECTONEMA-BORYANUM

  B MIKAMI; S IDA

  BIOCHIMICA ET BIOPHYSICA ACTA, 1984, Peer-reviewed

  
• PURIFICATION AND CHARACTERIZATION OF ASSIMILATORY NITRATE REDUCTASE FROM THE CYANOBACTERIUM PLECTONEMA-BORYANUM

  S IDA; B MIKAMI

  PLANT AND CELL PHYSIOLOGY, 1983, Peer-reviewed

  
• INTERACTION OF NATIVE AND SH-MODIFIED BETA-AMYLASE OF SOYBEAN WITH CYCLOHEXADEXTRIN AND MALTOSE

  B MIKAMI; K NOMURA; Y MORITA

  JOURNAL OF BIOCHEMISTRY, 1983, Peer-reviewed

  
• LOCATION OF SH-GROUPS ALONG THE POLYPEPTIDE-CHAIN OF SOYBEAN BETA-AMYLASE

  B MIKAMI; Y MORITA

  JOURNAL OF BIOCHEMISTRY, 1983, Peer-reviewed

  
• CIRCULAR-DICHROISM AND THE CONFORMATIONAL PROPERTIES OF SOYBEAN BETA-AMYLASE

  E MORI; B MIKAMI; Y MORITA; B JIRGENSONS

  ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1981, Peer-reviewed

  
• CHEMICAL MODIFICATION OF SULFHYDRYL-GROUPS IN SOYBEAN BETA-AMYLASE

  B MIKAMI; S AIBARA; Y MORITA

  JOURNAL OF BIOCHEMISTRY, 1980, Peer-reviewed

  

Misc.

• 変調磁場により作製した蛋白質擬単結晶の構造解析

  三上文三

  第61回日本木材学会大会, 2011, Peer-reviewed

  
• アゼチジン-2-カルボン酸加水分解酵素のX線結晶構造解析

  三上文三

  日本農芸化学会2011年度大会, 2011, Peer-reviewed

  
• フレキシブルループに変異を導入したKlebsiella由来プルラナーゼの熱安定性

  三上文三

  日本農芸化学会2011年度大会, 2011, Peer-reviewed

  
• 結晶構造に基づくβ－アミラーゼの活性部位ループの機能解析

  三上文三

  日本農芸化学会2011年度大会, 2011, Peer-reviewed

  
• 苦味を制御する鶏卵抗体の作製とその評価

  三上文三

  日本農芸化学会2011年度大会, 2011, Peer-reviewed

  
• 出芽酵母Pos5が高いNADHキナーゼ活性を示す構造要因

  三上文三

  日本農芸化学会2011年度大会, 2011, Peer-reviewed

  
• 酵母Pichia pastorisを用いたハイスループット発現系構築法の開発：膜タンパク質・分泌タンパク質への応用

  三上文三

  日本農芸化学会2011年度大会, 2011, Peer-reviewed

  
• 分子モデルに基づいた制限酵素EcoT38Iの機能解析

  三上文三

  日本農芸化学会2011年度大会, 2011, Peer-reviewed

  
• ギ酸オキシダーゼへの変異導入によるフラビン補酵素構造変化

  三上文三

  日本農芸化学会2011年度大会, 2011, Peer-reviewed

  
• ニューロセルピンのリフォールディングとポリマー化経路

  三上文三

  日本農芸化学会2011年度大会, 2011, Peer-reviewed

  
• 生物種を超えて共通するフェリチンにおける鉄貯蔵機構

  三上文三

  日本農芸化学会2011年度大会, 2011, Peer-reviewed

  
• オボアルブミンにおけるアミロイド形成コアの探索

  三上文三

  日本農芸化学会2011年度大会, 2011, Peer-reviewed

  
• 甘味タンパク質ソーマチンの甘味発現機構

  三上文三

  日本農芸化学会2011年度大会, 2011, Peer-reviewed

  
• 連鎖球菌由来不飽和グルクロニルヒドロラーゼのグリコサミノグリカン二糖に対する硫酸基認識

  三上文三

  日本農芸化学会2011年度大会, 2011, Peer-reviewed

  
• 結晶構造に基づくプロテイングルタミナーゼの酵素機能解析

  三上文三

  日本農芸化学会2011年度大会, 2011, Peer-reviewed

  
• 植物フェリチンの立体構造解析より明らかとなった種を超えて共通するフェリチンにおける鉄貯蔵機構

  増田太郎; 後藤文之; 吉原利一; 三上文三

  日本蛋白質科学会年会プログラム・要旨集, 15 May 2010, Peer-reviewed

  
• X-Ray crystallographic analysis of mung bean 8S globulin.

  Mikami, B; Moriya, T; Tandang, M; Masuda, T; Utsumi S

  SPring-8 User Experiment Report 2010A1309, 2010., 2010

  
• リゾチームの擬単結晶のX線構造解析

  三上文三

  第59回高分子学会年次大会, 2010, Peer-reviewed

  
• ギ酸オキシダーゼへの変異の導入によるフラビン補酵素の構造変化

  三上文三

  北陸合同バイオシンポジウム2010, 2010, Peer-reviewed

  
• GMCオキシダーゼファミリーに属するギ酸オキシダーゼの活性部位には8-formyl FAD とHis-Arg 対が存在する

  三上文三

  北陸合同バイオシンポジウム2010, 2010, Peer-reviewed

  
• Crystal Structure of Protein-Glutaminase and Its Pro-Structures Similar to Enzyme-Substrate Complex

  Bunzo Mikami

  26th European Crystallographic Meeting, 2010, Peer-reviewed

  
• ギ酸オキシダーゼの立体構造と補酵素の解明

  三上文三

  日本生化学会北陸支部第28回大会, 2010, Peer-reviewed

  
• Sphingomonas属細菌A1株由来ペプチドグリカン分解酵素の立体構造に基づく触媒機構解析

  三上文三

  日本農芸化学会関西支部大会（第466回講演会）, 2010, Peer-reviewed

  
• 硫酸化グリコサミノグリカンに作用する連鎖球菌由来不飽和グルクロニルヒドロラーゼの基質認識機構

  三上文三

  日本農芸化学会関西支部大会（第466回講演会）, 2010, Peer-reviewed

  
• ニューロセルピンのリフォールディングとポリマー化の関わり

  三上文三

  日本蛋白質科学会第10回年会, 2010, Peer-reviewed

  
• X-ray single crystal analysis of a pseudo single crystal of protein microcrystalline powders

  Bunzo Mikami

  Pacifichem 2010, 2010, Peer-reviewed

  
• 結晶構造比較による11Sグロブリンの保存性と多様性の解析

  三上文三

  日本農芸化学会2010年度大会, 2010, Peer-reviewed

  
• プルラナーゼの保存領域３を含むループのinduced-fit motionは活性発現に必要か？

  三上文三

  日本農芸化学会2010年度大会, 2010, Peer-reviewed

  
• ダイズβ－アミラーゼのフレキシブルループ変異体の結晶構造

  三上文三

  日本農芸化学会2010年度大会, 2010, Peer-reviewed

  
• Mgイオン存在下で再生したCold-active protein tyrosine phosphataseの立体構造解析

  三上文三

  日本農芸化学会2010年度大会, 2010, Peer-reviewed

  
• メダカの分泌タンパク質αアミラーゼの酵母を用いた発現系の構築

  三上文三

  日本農芸化学会2010年度大会, 2010, Peer-reviewed

  
• 甘味タンパク質ソーマチンのX線結晶構造解析 (2)アルギニン82の重要性

  三上文三

  日本農芸化学会2010年度大会, 2010, Peer-reviewed

  
• 植物フェリチンの立体構造と鉄貯蔵機構の解析

  三上文三

  日本農芸化学会2010年度大会, 2010, Peer-reviewed

  
• 天然型ニューロセルピンのポリマー化機構

  三上文三

  日本農芸化学会2010年度大会, 2010, Peer-reviewed

  
• S-オボアルブミン形成におけるセリン残基の役割：D-セリン生成の可能性に関するタンパク質工学的研究

  三上文三

  日本農芸化学会2010年度大会, 2010, Peer-reviewed

  
• Serpinスーパーファミリーに高度に保存されたTrp残基のインヒビター活性発現における役割

  三上文三

  日本農芸化学会2010年度大会, 2010, Peer-reviewed

  
• アルギン酸輸送ABCトランスポーターの機能解析と予備的X線結晶構造解析

  三上文三

  日本農芸化学会2010年度大会, 2010, Peer-reviewed

  
• ヒト苦味受容体（hTAS2R16）の酵母を用いた発現系構築

  三上文三

  日本農芸化学会2010年度大会, 2010, Peer-reviewed

  
• Role of induced-fit motion in the active site of Klebsiella pullulanase

  Bunzo Mikami

  日本生化学会83回大会, 2010, Peer-reviewed

  
• ビタミンB6 分解酵素4-pyridoxolactonase のX 線結晶構造解析

  三上文三

  日本生化学会83回大会, 2010, Peer-reviewed

  
• 結晶構造から明らかにされた制限酵素EcoT38I のユニークな反応機構

  三上文三

  日本生化学会83回大会, 2010, Peer-reviewed

  
• 甘味関連タンパク質の機能発現に関する高分解能構造解析.

  Masuda, T; Kigo, S; Mikami, B; Kitabatake, N

  SPring-8 User Experiment Report 2009B1379, 2009., 2009

  
• X-ray crystallographic analysis of phytoferritin subunit from soybean

  Masuda, T; Goto, F; Yoshihara, T; Yoshimura, T; Mikami, B

  SPring-8 User Experiment Report 2009A1249, 2009., 2009

  
• アルギン酸代謝に関わる細菌由来α-ケト酸還元酵素のX線結晶構造

  三上文三

  日本農芸化学会関西支部第458回講演会, 2009, Peer-reviewed

  
• ニワトリ卵白リゾチーム擬単結晶のX線構造解析

  三上文三

  第4回日本磁気科学会年会, 2009, Peer-reviewed

  
• プロ型プロテイングルタミナーゼのX線結晶構造解析

  三上文三

  第56回日本生化学会近畿支部例会, 2009, Peer-reviewed

  
• X 線結晶構造解析により明らかとなった野生型ニューロセルピンのポリマー化要因

  三上文三

  日本蛋白質科学会第9回年会, 2009, Peer-reviewed

  
• endo‒ 1,3 ‒β‒glucanase の構造や機能における触媒残基とCa2+ の役割

  三上文三

  日本蛋白質科学会第9回年会, 2009, Peer-reviewed

  
• ダイズフェリチン（SFER4）24 量体のX 線結晶構造解析

  三上文三

  日本蛋白質科学会第9回年会, 2009, Peer-reviewed

  
• Structural determinants responsible for mode of action in polysaccharide lyases for biofuel production

  Bunzo Mikami

  日本生化学会82回大会, 2009, Peer-reviewed

  
• A novel streptococcal unsaturated glucuronyl hydrolases specific for sulfated glycosaminoglycans

  Bunzo Mikami

  日本生化学会82回大会, 2009, Peer-reviewed

  
• Coenzyme-binding mode in α-keto acid reductase responsible for alginate metabolism

  三上文三

  日本生化学会82回大会, 2009, Peer-reviewed

  
• 2T6p-17 出芽酵母Pos5 が強力なNADH キナーゼ活性を示し得る構造要因

  三上文三

  日本生化学会82回大会, 2009, Peer-reviewed

  
• ニューロセルピンの結晶構造により明らかとなった複数のポリマー化経路

  三上文三

  日本生化学会82回大会, 2009, Peer-reviewed

  
• 好冷菌Shewanella sp.が産生するCold-active alkaline phosphatase の結晶構造

  三上文三

  日本生化学会82回大会, 2009, Peer-reviewed

  
• ニューロセルピンのX線結晶構造解析

  三上文三

  日本農芸化学会2009年度大会, 2009, Peer-reviewed

  
• デンプン枝切り酵素のサブサイト－２および＋２の役割

  三上文三

  日本農芸化学会2009年度大会, 2009, Peer-reviewed

  
• 褐藻アルギン酸の糖化に関わる細菌由来エキソ型リアーゼの構造・機能相関

  三上文三

  日本農芸化学会2009年度大会, 2009, Peer-reviewed

  
• オボアルブミンのアミロイド線維形成:内在性ペプチドの添加効果

  三上文三

  日本農芸化学会2009年度大会, 2009, Peer-reviewed

  
• ファミリーGH73ペプチドグリカン分解酵素のX線結晶構造

  三上文三

  日本農芸化学会2009年度大会, 2009, Peer-reviewed

  
• ファミリーPL-14多糖リアーゼvAL-1(S)の構造・機能相関

  三上文三

  日本農芸化学会2009年度大会, 2009, Peer-reviewed

  
• 細菌由来アルギン酸輸送ABCトランスポーターの発現・機能解析と結晶化

  三上文三

  日本農芸化学会2009年度大会, 2009, Peer-reviewed

  
• Chryseobacterium proteolyticum 9670株由来プロテイングルタミナーゼの結晶構造解析

  三上文三

  日本農芸化学会2009年度大会, 2009, Peer-reviewed

  
• Streptomyces mobaraensis 由来プロ体トランスグルタミナーゼの結晶構造解析

  三上文三

  日本農芸化学会2009年度大会, 2009, Peer-reviewed

  
• 制限酵素EcoT38Iの結晶構造解析

  三上文三

  日本農芸化学会2009年度大会, 2009, Peer-reviewed

  
• The PD-1/PD-L1 complex resembles the antigen-binding Fv domains of antibodies and T cell receptors

  David Yin-Wei Lin; Yoshimasa Tanaka; Masashi Iwasaki; Apostolos G. Gittis; Hua-Poo Su; Bunzo Mikami; Taku Okazaki; Tasuku Honjo; Nagahiro Minato; David N. Garboczi

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Feb. 2008, Peer-reviewed

  
• Molecular mechanism of the structural change of egg white proteins.

  Takahashi, N; Mikami, B; Oe, T

  SPring-8 User Experiment Report 2008A1266, 2008., 2008

  
• X-Ray crystallographic analysis of a type II restriction endonuclease, R.EcoT38I, from Escherichia coli TH38.

  Kita, K; Nagao, J; Mikami, B

  SPring-8 User Experiment Report 2008A1219, 2008., 2008

  
• 枯草菌による植物細胞壁の分解:多糖ラムノガラクツロナンリアーゼYesWのX線結晶構造

  三上文三

  日本農芸化学会関西支部第449回講演会, 2008, Peer-reviewed

  
• サルモネラ菌由来マンノースイソメラーゼYihSの機能と立体構造

  三上文三

  日本農芸化学会関西支部第454回講演会, 2008, Peer-reviewed

  
• 新規アラビノース代謝経路に関与するL-2-ケト-3-デオキシアラボネート脱水酵素の結晶構造解析

  嶋田直子; 三上文三; 渡邉誠也; 小瀧努; 牧野圭祐

  第30回日本分子生物学会大会第80回日本生化学会大会合同大会，横浜，2007.12.11-15, 2007, Peer-reviewed

  
• 新規アラビノース代謝経路に関与する酵素L-2-keto-3-deoxyarabonate dehydrataseの結晶構造解析

  嶋田直子; 渡邉誠也; 小瀧努; 三上文三; 牧野圭祐

  京都大学21世紀COEプログラム「環境調和型エネルギーの研究教育拠点形成」成果発表会，京都，2007.3.12-13, 2007, Peer-reviewed

  
• バイオマス高効率変換酵素の立体構造解明および機能変換

  松井絢子; 依田香子; 渡邉誠也; 小瀧努; 三上文三; 牧野圭祐

  京都大学21世紀COEプログラム「環境調和型エネルギーの研究教育拠点形成」成果発表会，京都，2007.3.12-13, 2007, Peer-reviewed

  
• Crystal structure of soybean 11S globulin: Glycinin A3B4 homohexamer

  M Adachi; J Kanamori; T Masuda; K Yagasaki; K Kitamura; B Mikami; S Utsumi

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Jun. 2003, Peer-reviewed

  

Presentations

• Phenoloxidases and hemocyanins are similar but distinct proteins that belong to type 3 copper protein family

  Taro MASUDA; Tatsuki KAWAUCHI; Yasuyuki MATOBA; Haruhiko TOYOHARA; Bunzo MIKAMI

  The 2018 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, 17 Mar. 2018

  
• Effects of loop regions in thaumatin on elicitation of sweetness as well as heat stability

  Mayuko MITSUMOTO; Bunzo MIKAMI; Fumito TANI; Tetsuya MASUDA

  The 2018 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, 17 Mar. 2018

  
• Crystallographic analysis of pH dependency of β-amylase(E380A)/substrate complex with capillary crystals

  Bunzou Mikami; Motoyasu Adachi; Youna Kang; Kimihiko Mizutani; Nobuyuki Takahashi

  The 2018 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, 17 Mar. 2018

  
• The activation mechanism of GA metabolic enzyme in rice, revealed by MD simulation

  Sayaka TAKEHARA; Bunzo MIKAMI; Shun SAKURABA; Makoto MATSUOKA; Miyako UEGUCHI; TANAKA

  The 2018 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, 16 Mar. 2018

  
• Elucidation of mechanism of interaction between Klebsiella pneumoniae pullulanase and cyclodextrin

  Naoki SAKA; Keiichiro TANAKA; Konami YAMANO; Kimihiko MIZUTANI; Nobuyuki TAKAHASHI; Hiroyuki IWAMOTO; Bunzo MIKAMI

  The 2018 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, 16 Mar. 2018

  
• A bacterial protein involved in the import of glycosaminoglycans adopts two types of substrate-bound closed conformations

  Sayoko OIKI; Masahiro SATO; Reiko KAMOCHI; Bunzo MIKAMI; Kousaku MURATA; Wataru

  The 2018 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, 16 Mar. 2018

  
• X-ray crystal structural analysis of a high-fidelity mutant of EcoO109I restriction endonuclease in complex with cognate DNA

  Yukio WATANABE; Bunzo MIKAMI; Keiko KITA

  The 2018 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry, 16 Mar. 2018

  
• Domain dynamics of bacterial solute-binding protein involved in import of glycosaminoglycans

  Sayoko OIKI; Reiko KAMOCHI; Bunzo MIKAMI; Kousaku MURATA; Wataru HASHIMOTO

  The 2017 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 20 Mar. 2017

  
• X-ray crystallography and functional analysis of a snail cellulase EG27II

  Taisuke NOMURA; Kimihiko MIZUTANI; Hisamu IWASE; Nobuyuki TAKAHASHI; Bunzo MIKAMI

  The 2017 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 19 Mar. 2017

  
• Crystallographic analysis of structural change of β-amylase/substrate complex caused by pH

  Bunzou Mikami; Kazuhiro Kawamura; Motoyasu Adachi; Youna Kang; Kimihiko Mizutani; Nobuyuki Takahashi

  The 2017 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 18 Mar. 2017

  
• Structural and functional analyses of gibberellin 3-oxidase and 2-oxidase in rice

  Sayaka TAKEHARA; Bunzo MIKAMI; Kyosuke KAWAI; Makoto MATSUOKA; Miyako UEGUCHI; TANAKA

  The 2017 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 18 Mar. 2017

  
• Elicitation sweetness of sweet taste protein thaumatin

  Mayuko MITSUMOTO; Kyohei OKUBO; Bunzo MIKAMI; Fumito TANI; Tetsuya MASUDA

  The 2017 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 18 Mar. 2017

  
• The enhancement of bitter sensitivity by the antibody against a bitter taste receptor

  Atsushi DAN; Kaho KANATANI; Sohei SATO; Kimihiko MIZUTANI; Bunzo MIKAMI; Kenji MAEHASHI; Takashi IINO; Kenji HORIE; Mujo KIM; Yukako HAYASHI

  The 2017 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 18 Mar. 2017

  
• pH Dependent conformational change of bound maltose observed in the crystal of β-amylase at room temperature

  MIKAMI Bunzo

  30th Europian Crystallographic Meeting, 01 Sep. 2016

  
• Crystallographic analysis of structural change of β-amylase/maltose complex caused by pH

  Bunzou Mikami; Kazuhiro Kawamura; Motoyasu Adachi; Youna Kang; Kimihiko Mizutani; Nobuyuki Takahashi

  The 2016 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 30 Mar. 2016

  
• Structural determinants for coenzyme requirement in reductases responsible for metabolism of acidic polysaccharides

  Ryuichi TAKASE; Yukie MARUYAMA; Sayoko OIKI; Bunzo MIKAMI; Kousaku MURATA; Wataru HASHIMOTO

  The 2016 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 30 Mar. 2016

  
• Structural and functional analysis of a sporulation protein Spo0M from Bacillus subtilis

  Yo SONODA; Kimihiko MIZUTANI; Bunzo MIKAMI

  The 2016 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 29 Mar. 2016

  
• Oxidative half-reaction of formate oxidase using nitrite as an electron acceptor

  Naoto Tanabe; Daijyu Doubayashi; Kohei Nakagaki; Masaya Oki; Bunzo Mikami; Hiroyuki Uchida

  The 2016 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 29 Mar. 2016

  
• Gibberellin metabolism is promoted by tetramerization of gibberellin 2-oxidase in rice

  Sayaka TAKEHARA; Bunzo MIKAMI; Toru KASHIO; Makoto MATSUOKA; Miyako UEGUCHI(TANAKA

  The 2016 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 29 Mar. 2016

  
• A pathogenic bacterial ABC transporter involved in the import of animal extracellular matrix, glycosaminoglycan

  Sayoko OIKI; Yukie MARUYAMA; Bunzo MIKAMI; Kousaku MURATA; Wataru HASHIMOTO

  The 2016 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 28 Mar. 2016

  
• Structural and functional analyses for T756R and dG mutants of Soybean-Lipoxygenase

  Yusuke HIOKI; Tsutomu NAGAYA; Bunzo MIKAMI; Nobuyuki TAKAHASHI; Kimihiko MIZUTANI

  The 2016 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 28 Mar. 2016

  
• A Hypersweet sweet-tasting protein, thaumatin

  Tetsuya Masuda; Keisuke Ohta; Naoko Ojiro; Kazuki Murata; Bunzo Mikami; Fumito Tani; Piero Temussi; Naofumi Kitabatake

  The 2016 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 28 Mar. 2016

  
• Preparation and characterization of the RNase H domain of MMLV reverse transcriptase

  Kosaku NISHIMURA; Kanta YOKOKAWA; Kosuke FUKATSU; Ikumi KUZE; Tetsuro HISAYOSHI; Atsushi KONISHI; Bunzo MIKAMI; Kenji KOJIMA; Kiyoshi YASUKAWA

  The 2015 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 29 Mar. 2015

  
• X-ray crystallographic analysis of glucoamylase from Aspergillus oryzae

  Emi Nagayoshi; Kimihiko Mizutani; Nobuyuki Takahashi; Bunzo Mikami; Yukio Takii

  The 2015 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 28 Mar. 2015

  
• Crystal structure of β-amylase/maltose complex in different pH conditions

  Bunzou Mikami; Kazuhiro Kawamura; Motoyasu Adachi; Kimihiko Mizutani; Nobuyuki Takahashi

  The 2015 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 28 Mar. 2015

  
• Degradation of mammalian extracellular matrices, glycosaminoglycans, by streptococci: Reaction mechanism of unsaturated glucuronyl hydrolase

  Yusuke Nakamichi; Sayoko Oiki; Bunzo Mikami; Kousaku Murata; Wataru Hashimoto

  The 2015 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 28 Mar. 2015

  
• Structural analysis of sweet-tasting protein, thaumatin by serial femtosecond crystallography

  Tetsuya Masuda; Eriko Nango; Michihiro Sugahara; Mamoru Suzuki; Kensuke Tono; Yasumasa Joti; Takashi Kameshima; Jaehyun PARK; Changyong SONG; Takaki Hatsui; Makina Yabashi; Kyohei Okubo; Bunzo Mikami; Naofumi Kitabatake; Fumito Tani; So Iwata

  The 2015 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 28 Mar. 2015

  
• Polysaccharide recognition by bacterial solute-binding protein dependent ABC transporter

  Yukie MARUYAMA; Akira TATEISHI; Ai KANEKO; Bunzo MIKAMI; Wataru HASHIMOTO; Kousaku MURATA

  The 2015 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 27 Mar. 2015

  
• UV absorption spectroscopic and X-ray crystallographic analysis of the binding of L-lysine with lysyl-tRNA synthetase and insights into the induced-fit mechanism of the binding

  Teisuke Takita; Bunzo Mikami; Kuniyo Inouye

  The 2015 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 27 Mar. 2015

  
• Structural and functional analysis of gibberellin 2-oxidase in rice

  Sayaka TAKEHARA; Bunzo MIKAMI; Toru KASHIO; Makoto MATSUOKA; Miyako UEGUCHI; TANAKA

  The 2015 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 27 Mar. 2015

  
• Crystal structure of gibberellin biosynthesis and metabolism enzyme

  Sayaka TAKEHARA; Bunzo MIKAMI; Toru KASHIO; Makoto MATSUOKA; Miyako UEGUCHI-TANAKA

  The 2014 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 30 Mar. 2014

  
• Streptococcal metabolic mechanism of host extracellular matrices, glycosaminoglycans

  Wataru Hashimoto; Yukie Maruyama; Ryuichi Takase; Yusuke Nakamichi; Bunzo Mikami; Kousaku Murata

  The 2014 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 29 Mar. 2014

  
• Elucidation of reaction mechanism of soybean β-amylase by X-ray crystallography

  Bunzou Mikami; Youna Kang; Motoyasu Adachi; Kazuhiro Kawamura; Kimihiko Mizutani; Nobuyuki Takahashi

  The 2014 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 28 Mar. 2014

  
• Crystallographic analysis of a novel phenoloxidase from crustacean reveals the mechanism for functional difference among type 3 copper proteins

  Taro MASUDA; Kyosuke Momoji; Takashi Hirata; Bunzo Mikami

  The 2014 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 28 Mar. 2014

  
• The processing of the extension peptide accelerates the iron release from plant ferritin

  Taro MASUDA; Itaru Sugihara; Shin-ichiro Morimoto; Bunzo Mikami; Haruhiko Toyohara

  The 2013 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 27 Mar. 2013

  
• Structure/function relationship and application of pit-dependent ABC transporter involved in polysaccharide import

  Wataru Hashimoto; Yukie Maruyama; Bunzo Mikami; Kousaku Murata

  The 2013 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 27 Mar. 2013

  
• Formate oxidase has an activity of reducing nitrite

  Hiroyuki Uchida; Yuusuke Seko; Miri Kuroyanagi; Anna Wakaizumi; Masaya Oki; Akihiko Sakurai; Bunzou Mikami

  The 2013 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 26 Mar. 2013

  
• Production and the assessment of the chicken egg yolk antibody against the bitter taste receptor hT2R16

  Sohei SATO; Hirotoshi FUJITO; Kimihiko MIZUTANI; Bunzo MIKAMI; Akira FUSHIKI; Kenji MAEHASHI; Yusuke YAMASHITA; Takashi IINO; Kenji HORIE; Mujo KIM; Yasuki MATSUMURA; Yukako HAYASHI

  The 2013 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 26 Mar. 2013

  
• Elucidation of reaction mechanism of β-amylase by X-ray crystallography

  Bunzou Mikami; Youna Kang; Motoyasu Adachi; Kazuhiro Kawamura; Kimihiko Mizutani; Nobuyuki Takahashi

  The 2013 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 25 Mar. 2013

  
• Crystal structure and substrate import of bacterial alginate ABC transporter

  Yukie Maruyama; Ai Kaneko; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  The 2013 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 25 Mar. 2013

  
• Reaction mechanism of azetidine-2-carboxylate hydrolase based on complex crystal structure

  Mayuko TOYODA; Keiji JITSUMORI; Lawrence P. WACKETT; Nobuyoshi ESAKI; Tatsuo KURIHARA; Bunzo MIKAMI

  The 2013 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 25 Mar. 2013

  
• X-ray crystallography of bacterial α-keto acid reductases responsible for polyuronate metabolism

  Ryuichi Takase; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  The 2012 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 25 Mar. 2012

  
• Folding intermediate on the polymerization of ovalbumin

  Chieri Sugita; Nobuyuki Takahashi; Bunzo Mikami

  The 2012 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 24 Mar. 2012

  
• The N-terminal region of phytoferritin is related to the association of multimer of phytoferritin

  Taro MASUDA; Shin-ichito MORIMOTO; Bunzo MIKAMI; Haruhiko TOYOHARA

  The 2012 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 24 Mar. 2012

  
• The function of Arg554 in Formate Oxidase from Aspergillus oryzae RIB40

  Anna Wakaizumi; Takumi Ootake; Masaya Oki; Hiroyuki Uchida; Bunzou Mikami

  The 2012 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 24 Mar. 2012

  
• X-ray crystallographic analysis of bacterial ABC transporter involved in import of alginate polysaccharide

  Yukie Maruyama; Takafumi Itoh; Yu Nishitani; Bunzo Mikami; Wataru Hashimoto; Kousaku Murata

  The 2012 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 24 Mar. 2012

  
• Crystal structure of recombinant medaka alpha-amylase expressed in yeast Pichia pastoris

  Kimihiko Mizutani; Mayuko Toyoda; Soshi Yoshioka; Yuichiro Otake; Bunzo Mikami

  The 2012 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 23 Mar. 2012

  
• Characterization of flexible loop mutant of bacterial pullulanase

  Hiroyuki Iwamoto; Takanori Sato; Dominggus Malle; Bunzo Mikami

  The 2012 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 23 Mar. 2012

  
• Studies on the sweetness determinants in thaumatin by x-ray crystallographic analysis at atomic resolution

  Tetsuya Masuda; Keisuke Ohta; Bunzo Mikami; Naofumi Kitabatake; Fumito Tani

  The 2012 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 23 Mar. 2012

  
• Elucidation of reaction mechanism of beta-amylase by X-ray crystallography

  Bunzo Mikami; Kakuji Okumura; Sousuke Yamada; Motoyasu Adachi; Kimihiko Mizutani; Nobuyuki Takahashi

  The 2012 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 23 Mar. 2012

  
• Structural properties of a thermostable β-1,3-xylanase from marine hyperthermophile Thermotoga neapolitana

  Kiyoshi Yamazaki; Fumiyoshi Okazaki; Chiaki Ogino; Akihiko Kondo; Bunzo Mikami; Yoichi Kurebayashi; Hiroki Tsuruta

  The 2012 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 23 Mar. 2012

  
• Functional analysis of Protein-glutaminase based on mutant crystal structures

  Yukiko MAKI; Ryouta Hashizume; Kimihiko Mizutani; Nobuyuki Takahashi; Hirotaka Matsubara; Akiko Matsunaga; Shoutaro Yamaguchi; Bunzo MIKAMI

  The 2011 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistr, 27 Mar. 2011

  
• 結晶構造に基づいたアミラーゼの触媒機能の解明

  第11回関西グライコサイエンスフォーラム, 2010

  
• 実験室系発生器・放射光施設両方を利用した効果的なデータ収集と構造解析

  日本蛋白質科学会第9回年会, 2009

  

Books and Other Publications

• Lehninger Principles ob Biochemistry 6th edition

  MIKAMI Bunzo, Joint translation, pp.163-223

  Hirokawa Publishing CO., Feb. 2015, Not refereed

  
• Crystallography in Japan (II)

  MIKAMI Bunzo, Contributor, pp. 376

  Crystallographic Society Japan, Jul. 2014, Not refereed

  
• 大豆のすべて 北村啓介 編集 第2節2.1 グロブリンたんぱく質 一時構造、二次構造、三次構造

  三上 文三, Contributor, pp.115-121

  サイエンス フォーラム, Feb. 2010, Not refereed

  
• X-Ray crystallographic analysis of peanut conarachin.

  Mikami, B; Urabe, H; Tandang, M; Masuda, T; Maruyama, N; Utsumi S

  Spring-8 User Experiment Report 2010A6538, 2010., 2010, Not refereed

  
• X-Ray crystallographic analysis of vacuolar sorting receptor (VSR) from soybean cells .

  Mikami, B; Maruyama, N; Utsumi S

  SPring-8 User Experiment Report 2010B6538, 2010., 2010, Not refereed

  
• X-Ray crystallographic analysis of a mutant mung bean 8S globulin.

  Mikami, B; Yamazaki, Y; Maruyama, N; Tandang, M; Masuda, T; Itoh, T; Utsumi S

  SPring-8 User Experiment Report 2010B1456, 2010., 2010, Not refereed

  
• グロブリンタンパク質．大豆のすべて（喜多村啓介他編）

  三上文三; 丸山伸之; 内海 成

  p. 115-121、サイエンスフォーラム、東京、2010., 2010, Not refereed

  
• X-Ray crystallographic analysis of mutant beta-amylase/maltose complex.-Titration of two loop conformations of V99N by maltose-

  Mikami, B; Tanabe, A; Hashizume, R; Yoshimura, T

  Pring-8 User Experiment Report 2010A1489, 2010., 2010, Not refereed

  
• X-Ray crystallographic analysis of mutant beta-amylase/maltose complex.-Titration of two loop conformations of K295A by maltose-.

  Mikami, B; Tanabe, A; Adachi, M; Okumura, T

  SPring-8 User Experiment Report 2010B1181, 2010., 2010, Not refereed

  
• X-Ray crystallographic analysis of mung bean 8S globulin.

  Mikami, B; Moriya, T; Tandang, M; Masuda, T; Utsumi S

  SPring-8 User Experiment Report 2010A1309, 2010., 2010, Not refereed

  
• X-ray Crystal Structure of Sphingomonas sp. A1 α-Keto Acid Reductase for Alginate Metabolism.

  Takase, R; Ochiai, A; Nakamichi, Y; Itoh, T; Ogura, K; Mikami, B; Hashimoto, W; Murata, K

  SPring-8 User Experiment Report 2009A1179, 2009., 2009, Not refereed

  
• X-Ray crystallographic analysis of plant seed globulins.

  Mikami, B; Tandang, M; Maryuama, N; Itoh, T; Takehara, S; Utsumi, S

  SPring-8 User Experiment Report 2009B6935, 2009., 2009, Not refereed

  
• X-Ray crystallographic analysis of pro legumin from amaranth.

  Mikami, B; Tandang, M; Yoshimura, T; Hashizume, R; Masuda, T; Maruyama, N

  SPring-8 User Experiment Report 2009B1299, 2009., 2009, Not refereed

  
• X-ray crystallographic analysis of mutant b-amylase/maltose complex. -Titration of two loop conformations of G97A by maltose-.

  Mikami, B; Toyoda, Y; Saito, T; Adachi, M; Utsumi, S

  SPring-8 User Experiment Report 2009A1168, 2009., 2009, Not refereed

  
• X-ray crystallographic analysis of loop-less beta-amylase/maltose complex.

  Mikami, B; Adachi, M; Tanabe, A; Hashizume, R; Yoshimura, T; Utsumi, S

  SPring-8 User Experiment Report 2009B1461, 2009., 2009, Not refereed

  
• Crystal structure of the metallopeptidase family M16 enzyme from Sphingomonas sp. strain A1.

  Maruyama, Y; Chuma, A; Ochiai, A; Ogura, K; Nakamichi, Y; Mikami, B; Hashimoto, W; Murata, K

  SPring-8 User Experiment Report 2009B1192, 2009., 2009, Not refereed

  
• 植物細胞壁多糖からのバイオエタノール生産に関わる細菌酵素系の構造生物学.

  Takase, R; Ochiai, A; Itoh, T; Ogura, K; Mikami, B; Hashimoto, W; Murata, K

  SPring-8 User Experiment Report 2009B1177, 2009., 2009, Not refereed

  
• 味覚関連タンパク質の機能発現に関する構造生物学的解析

  Masuda, T; Kigo, S; Ohta, K; Mikami, B; Kitabatake, N

  SPring-8 User Experiment Report 2009A1096, 2009., 2009, Not refereed

  
• X-Ray crystallographic analysis of peanut conarachin.

  Mikami, B; Urabe, H; Yoshimura, T; Hashizume, R; Maruyama, N; Utsumi, S

  SPring-8 User Experiment Report 2009A1165, 2009., 2009, Not refereed

  
• X-ray structural analysis of the metallopeptidase family M16 enzyme.

  Maruyama, Y; Chuma, A; Ochiai, A; Ogura, K; Nakamichi, Y; Mikami, B; Hashimoto, W; Murata, K

  SPring-8 User Experiment Report 2009A1329, 2009., 2009, Not refereed

  
• 甘味関連タンパク質の機能発現に関する高分解能構造解析.

  Masuda, T; Kigo, S; Mikami, B; Kitabatake, N

  SPring-8 User Experiment Report 2009B1379, 2009., 2009, Not refereed

  
• X-ray crystallographic analysis of phytoferritin subunit from soybean

  Masuda, T; Goto, F; Yoshihara, T; Yoshimura, T; Mikami, B

  SPring-8 User Experiment Report 2009A1249, 2009., 2009, Not refereed

  
• X-Ray crystallographic analysis of soybean ferritin variant E173A.

  Masuda, T; Kishi, N; Mikami, B; Goto, F; Yoshihara, T

  SPring-8 User Experiment Report 2009A6935, 2009., 2009, Not refereed

  
• Structural biology of polysaccharides-degrading enzymes: X-ray crystallographic analysis of bacterial peptidoglycan hydrolase.

  Ochiai, A; Itoh, T; Ogura, K; Mikami, B; Hashimoto, W; Murata, K

  SPring-8 User Experiment Report 2008A1322, 2008., 2008, Not refereed

  
• Structural Biology of Transport of Metal Ions in Living Body: X-ray Crystallographic Analyses of Transferrin and Transferrin-receptor as a Fundamental Research for Drug-delivery.

  Mizutani, K; Toyoda, M; Mikami, B

  SPring-8 User Experiment Report 2008B1023, 2008., 2008, Not refereed

  
• Crystal Structure of Alginate-Binding Protein (AlgQ1) in Complex with Unsaturated Mannuronic Acid Trisaccharide.

  Itoh, T; Ogura, K; Nakamichi, Y; Mikami, B; Hashimoto, W; Murata, K

  SPring-8 User Experiment Report 2008A1191, 2008., 2008, Not refereed

  
• Molecular mechanism of the structural transition of egg white proteins.

  Takahashi, N; Mikami, B; Mizutani, K; Toyoda, M

  SPring-8 User Experiment Report 2008B1297, 2008., 2008, Not refereed

  
• X-Ray crystallographic analysis of cleaved phaseolin.

  Mikami, B; Park, K; Maruyama, N; Shutov, A; Utsumi, S

  SPring-8 User Experiment Report 2008B1574, 2008., 2008, Not refereed

  
• X-ray crystallographic analysis of beta-amylase/maltose complex -Titration of two loop conformations by maltose-.

  Mikami, B; Kang, Y.N; Tanabe, A; Utsumi, S

  SPring-8 User Experiment Report 2008A1252, 2008., 2008, Not refereed

  
• X-Ray crystallographic analysis of soybean ferritin.

  Masuda, T; Mikami, B; Goto, F; Yoshihara, T

  SPring-8 User Experiment Report 2008B6828, 2008., 2008, Not refereed

  
• Structural Insights into the Reaction mechanism of Coenzyme-Independent Mannose Isomerase.

  Itoh, T; Ochiai, A; Ogura, K; Mikami, B; Hashimoto, W; Murata, K

  SPring-8 User Experiment Report 2008A1119, 2008., 2008, Not refereed

  
• X-ray crystallographic analysis of mutant beta-amylase/maltose complex -Titration of two loop conformations of D101N and D101E by maltose-.

  Mikami, B; Tanabe, A; Utsumi, S

  SPring-8 User Experiment Report 2008B1522, 2008., 2008, Not refereed

  
• X-ray Crystal Structure of Sphingomonas sp. A1 Peptidoglycan Hydrolase Categorized to Family GH-73.

  Hashimoto, W; Ochiai, A; Itoh, T; Ogura, K; Mikami, B; Maruyama, Y; Murata, K

  SPring-8 User Experiment Report 2008A1112, 2008., 2008, Not refereed

  
• Crystal structure analysis of pea prolegumin.

  Mikami, B; Fukuda, T; Fukuda, C; Park, K; Maruyama, N; Utsumi, S

  SPring-8 User Experiment Report 2009B6935, 2009., 2008, Not refereed

  
• Molecular mechanism of the structural change of egg white proteins.

  Takahashi, N; Mikami, B; Oe, T

  SPring-8 User Experiment Report 2008A1266, 2008., 2008, Not refereed

  
• X-Ray crystallographic analysis of a type II restriction endonuclease, R.EcoT38I, from Escherichia coli TH38.

  Kita, K; Nagao, J; Mikami, B

  SPring-8 User Experiment Report 2008A1219, 2008., 2008, Not refereed

  
• Structure of β-amylase in enzyme chemistry and molecular biology of amylases and related enzymes

  三上 文三

  CRC Press Inc.,/,145-153, 1995, Not refereed

  
• Handbook of amylase and related enzymes their sources, isolation methods, properties and applications

  三上 文三

  Pergamon Press,, 1988, Not refereed

  

Works

• 文部科学省タンパク3000プロジェクト （４） タンパク質高次構造形成と機能発現

  From 2002

  
• 農業生物資源研究所イネゲノムプロジェクト タンパク質立体構造解析に基づく遺伝子機能の解明

  From 2002

  
• Rice genome project

  From 2002

  
• Protein 3000 project

  From 2002

  

Awards

• 1994

  農芸化学奨励賞

External funds: Kakenhi

• 動物細胞外粘液物質を介した腸内優占菌と宿主との相利共生機構の解明

  Grant-in-Aid for Scientific Research (B)

  Basic Section 38060:Applied molecular and cellular biology-related

  Kyoto University

  橋本 渉

  From 01 Apr. 2021, To 31 Mar. 2024, Granted

  腸内細菌叢;動物宿主;相利共生;ムチン;グリコサミノグリカン;微生物叢;常在機構;膜小胞;プロバイオティクス;Bacteroides;γ-アミノ酪酸

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H02156/
• Elucidation of dynamics and colonization mechanisms of microbiota targeting animal extracellular matrices and their application development

  Grant-in-Aid for Scientific Research (B)

  Basic Section 38060:Applied molecular and cellular biology-related

  Kyoto University

  Wataru Hashimoto

  From 01 Apr. 2018, To 31 Mar. 2021, Project Closed

  微生物叢;グリコサミノグリカン;常在機構;感染機構;プロバイオティクス;X線結晶構造解析;ムチン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18H02166/
• Functional analysis of food proteins and food-related enzymes.

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Bunzo Mikami

  From 01 Apr. 2016, To 31 Mar. 2019, Project Closed

  構造生物学;タンパク質工学;酵素工学;X線結晶構造解析;アミラーゼ;プルラナーゼ;プロテイングルタミナーゼ;糖質関連酵素;食糧関連酵素・タンパク質;トランスグルタミナーゼ;糖転移酵素;x線結晶構造解析;食糧関連酵素の構造生物学;食糧関連酵素のタンパク質工学;食糧関連酵素のＸ線結晶構造解析;タンパク質結晶構造解析

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16H04909/
• Structural life science of pathogenic bacterial systems targeting animal host extracellular matrix for colonization and infection

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Wataru HASHIMOTO

  From 01 Apr. 2015, To 31 Mar. 2018, Project Closed

  病原性細菌;細胞外マトリクス;グリコサミノグリカン;フォスフォトランスフェラーゼ系;ABCトランスポーター;分解・代謝酵素;遺伝子クラスター;X線結晶構造解析;連鎖球菌;連鎖桿菌;細胞外マトリックス;細菌;分解系;輸送系;宿主細胞外マトリクス;代謝酵素;動物細胞外マトリクス;酵素;輸送体;分解酵素

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15H04629/
• A new strategy to develop therapeutic agents for neurodegenerative diseases by structural biology

  Grant-in-Aid for Scientific Research (C)

  Osaka Prefecture University

  Maki Onda

  From 01 Apr. 2014, To 31 Mar. 2017, Project Closed

  neuroserpin;tPA;inhibitor;drug design;serpin;alzheimer;neurodegeneration;dementia

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26440079/
• Elucidation of molecular structure of wheat protein causing severe allergic symptoms

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  NOBUYUKI MARUYAMA

  From 01 Apr. 2014, To 31 Mar. 2017, Project Closed

  アレルゲン;小麦;エピトープ;食品;アレルギー;食物;植物;種子

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26282018/
• Development of a new metalloprotein based on the structure and function of metalloenzymes

  Grant-in-Aid for Challenging Exploratory Research

  Kyoto University

  Taro MASUDA

  From 01 Apr. 2012, To 31 Mar. 2015, Project Closed

  蛋白質;立体構造;金属;リガンド;メタロプロテイン;フェノールオキシダーゼ;キチナーゼ;フェリチン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24658287/
• Elucidation of mobile loop functions in food-related enzymes

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Bunzo MIKAMI

  From 01 Apr. 2012, To 31 Mar. 2015, Project Closed

  応用構造生物学;タンパク質工学;酵素工学;X線結晶構造解析;酵素反応機構;タンパク質結晶構造解析;構造生物学;タンパク質構造変化;食品関連酵素;酵素反応

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24380054/
• New strategy for the development of drugs to treat serpinopathies based on structural biology

  Grant-in-Aid for Scientific Research (C)

  Osaka Prefecture University

  Maki ONDA

  From 01 Apr. 2011, To 31 Mar. 2014, Project Closed

  serpin;conformational diseases;dementia;folding;neurodegeneration;drug design;neuroserpin;ovalbumin;serpins;Protein folding;Dementia;Protein Folding;Conformational Diseases;Serpin;脳神経疾患;生物物理;蛋白質;痴呆;フォールディング;神経変性;コンフォメーション病

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570195/
• Structure and function of complex of substrate-binding protein and macromolecule-specific ABC transpoter

  Grant-in-Aid for Scientific Research (B)

  Setsunan University;Kyoto University

  Kousaku MURATA

  From 01 Apr. 2011, To 31 Mar. 2014, Project Closed

  ABCトランスポーター;酸性多糖輸送;Sphingomonas属細菌;Ｘ線結晶構造;輸送体分子進化;X線結晶構造;基質結合タンパク質;アルギン酸;スフィンゴモナス属細菌;X線結晶構造解析;プロテオリポソーム再構成系;ヘテロ多糖;高分子輸送;アルギン酸結合タンパク質;ABCタンパク質

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23380049/
• Elucidation of mobile loop functions in food-related enzymes

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Bunzo MIKAMI

  From 01 Apr. 2009, To 31 Mar. 2012, Project Closed

  タンパク質工学;応用構造生物学;X線結晶構造解析;酵素反応機構;酵素;タンパク質構造変化;フレキシブルループ;酵素反応;構造生物学

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380064/
• Structure/function relationship and cell surface localization mechanism of bacterial flagelin

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Kousaku MURATA

  From 01 Apr. 2008, To 31 Mar. 2011, Project Closed

  フラジェリン;鞭毛;ペプチドグリカン加水分解酵素;メタロペプチダーゼ;X線結晶構造解析;体腔形成細菌;細菌細胞表層構造;アルギン酸;ペプチドグリカン加水分解楮;スフィンゴモナス属細菌;超チャネル;ペプチドグリカン構造;ペプチドグリカン分解酵素;X線結晶構造;フラジェリン進化

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380049/
• Studies on the molecular mechanism of shell formation especially focusing on the silk-like proteins

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Haruhiko TOYOHARA

  From 01 Apr. 2006, To 31 Mar. 2009, Project Closed

  バイオミネラリゼーション;マガキ;炭酸カルシウム;石灰化;クモ糸様タンパク質;凝集沈殿剤;貝殻;クモ糸様タンパク;ペプチド;クモ系;バイオミネラリゼーシ;カルシウム;外套膜

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380124/
• Loop technology of food related enzymes and food proteins

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Bunzo MIKAMI

  From 01 Apr. 2006, To 31 Mar. 2008, Project Closed

  ナノバイオ;酵素;タンパク質工学;可動ループ;X線結晶構造解析, nanobio;enzyme;protein eneineering;mobile loop;X-ray crystal analysis

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380067/
• タンパク質の「揺らぎ」と機能発現における水分子の役割

  Grant-in-Aid for Scientific Research on Priority Areas

  Science and Engineering

  Kyoto University

  村田 幸作

  From 01 Apr. 2006, To 31 Mar. 2008, Project Closed

  枯草菌;X線結晶構造解析;植物細胞壁;侵入・感染機構;不飽和ガラクツロニルヒドロラーゼ;ラムノガラクツロナン;水和;ビニルエーテル結合;連鎖球菌;細胞外マトリックス;グリコサミノグリカン;ヒアルロン酸リアーゼ;不飽和グルクロニルヒドロラーゼ;水和反応;反応機構

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18031018/
• Research of the structural factors for eliciting the highly catalytic efficiency of cold-active enzyme

  Grant-in-Aid for Scientific Research (C)

  Kobe University

  Hiroki TSURUTA

  From 01 Apr. 2005, To 31 Mar. 2007, Project Closed

  低温環境;低温活性酵素;フォスファターゼ;X線結晶構造解析;疎水性相互作用;低温活性機構;変異型酵素解析;高分解能X線結晶構造解析;変異体ライブラリー, low temperature environment;cold-active enzyme;phosphatase;X-ray structural analysis;hydrophobic interaction

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17613001/
• Molecular basis for cell surface architecture and evolution of bacterial flagellin by structure and unction analysis of the protein

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Kousaku MURATA

  From 01 Apr. 2005, To 31 Mar. 2008, Project Closed

  Sphingomonas;アルギン酸;フラジェリン;鞭毛;レセプター;X線結晶構造解析;体腔;T4ファージ;フィンガードメイン;鞭毛タンパク質;細菌細胞表層構造;Sphingomonas属細菌;鞭毛進化;細胞表層;襞構造, Sphingomonas;Alginate;Flagellin;Flagellum;Receptor;X-ray crystallography;Pit;T4 phage

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380053/
• Biochemical significance of the non-common amino acid sequences specifying the common functions of enzymes, polysaccharide lyases

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Kousaku MURATA

  From 01 Apr. 2002, To 31 Mar. 2005, Project Closed

  多糖リアーゼ;構造生物学;X線結晶構造解析;タンパク質構造機能相関;Sphingomonas属細菌;Bacillus属細菌;糖質加水分解酵素;糖質エピメラーゼ;翻訳後修飾機構;ヒドロラーゼ;エピメラーゼ;Sphingomonas;Bacillus;アルギン酸リアーゼ;翻訳後修飾;X線結晶構造;オートプロセシング;タンパク質・遺伝子の進化;キサンタンリアーゼ, Polysaccharide lyase;Structural biology;X-ray crystallography;Structure;function relationship of protein;Sphingomonas;Bacillus;Primary structure;Protein module

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14360052/
• Domain engineering of β-amylase

  Grant-in-Aid for Scientific Research (C)

  KYOTO UNIVERSITY

  Bunzo MIKAMI

  From 01 Apr. 2000, To 31 Mar. 2002, Project Closed

  β-アミラーゼ;X線結晶構造解析;至適pH;デンプン吸着ドメイン;タンパク質工学;至適pHの変換, β-amylase;protein engineering;X-ray crystallography;optimum pH;starch binding domain

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660077/
• 細菌細胞表層における体腔の高次空間構造と高分子輸送機構

  Grant-in-Aid for Scientific Research on Priority Areas (A)

  Kyoto University

  村田 幸作

  From 01 Apr. 2000, To 31 Mar. 2001, Project Closed

  ABCトランスポーター;高分子膜輸送;ABCタンパク質;結合タンパク質;アルギン酸;X線結晶構造解析;スフィンゴモナス;体腔

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12019237/
• Domain engineering of β-amylase

  Grant-in-Aid for Scientific Research (C)

  KYOTO UNIVERSITY

  Bunzo MIKAMI

  From 01 Apr. 1998, To 31 Mar. 2000, Project Closed

  β-アミラーゼ;タンパク質工学;X線結晶構造解析;澱粉吸着;澱粉吸着ドメイン, β-amylase;protein engineering;x-ray crystal analysis;starch binding;starch-binding domain

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660084/
• 鉄イオンによるトランスフェリンの分子スイッチ機構:高次構造変換過程のX線結晶構造解析

  Grant-in-Aid for Scientific Research on Priority Areas

  Kyoto University

  広瀬 正明

  From 01 Apr. 1997, To 31 Mar. 1998, Project Closed

  トランスフェリン;オボトランスフェリン;X線結晶構造解析;分子スイッチ;高次構造変換;鉄イオン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09235215/
• Protein engineering of beta-amylase

  Grant-in-Aid for Scientific Research (C)

  KYOTO UNIVERSITY

  Bunzo MIKAMI

  From 01 Apr. 1996, To 31 Mar. 1998, Project Closed

  β-アミラーゼ;X線結晶構造解析;タンパク質工学;生デンプン分解, beta-amylase;protein engineering;X-ray crystal analysis;thermostability

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660109/
• X-ray diffraction study and reaction mechanism of bacterial alginate lyase

  Grant-in-Aid for Scientific Research (C)

  KYOTO UNIVERSITY

  Kousaku MURATA

  From 01 Apr. 1995, To 31 Mar. 1997, Project Closed

  難治性細菌感染症;緑膿菌;アルギン酸;X線結晶構造解析;アルギン酸リアーゼ, Bacterial infection;Pseudomonas aeruginosa;Alginate;X-ray diffraction;Alginate lyase

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660111/
• Protein engineering of beta-amylase

  Grant-in-Aid for General Scientific Research (C)

  KYOTO UNIVERSITY

  Bunzo MIKAMI

  From 01 Apr. 1994, To 31 Mar. 1996, Project Closed

  β-アミラーゼ;タンパク質工学;X線結晶構造解析, beta-amylase;protein engineering;x-ray crystal analysis

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06660104/
• Analysis of beta-Amylase Action by X-Ray Crystallography

  Grant-in-Aid for international Scientific Research

  Kyoto University

  Bunzo MIKAMI

  From 01 Apr. 1994, To 31 Mar. 1995, Project Closed

  β-アミラーゼ;X線結晶構造解析;酵素基質複合体;酵素反応機構;フレキシブルループ, beta-amylase;X-ray crystallography;enzyme mechanism;enzyme-substrate complex

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044121/
• Functional properties and conformational changes of food proteins - Roles of molten globule state

  Grant-in-Aid for General Scientific Research (B)

  KYOTO UNIVERSITY

  Masaaki HIROSE

  From 01 Apr. 1993, To 31 Mar. 1996, Project Closed

  食品蛋白質;モンテングロビュール;食品機能特性;オボアルブミン;オボトランスフェリン;血清アルブミン;モルテングロビュール;タンパク構造変換;タンパク質フラグメント, Food proteins;Molten globule;Food functionality;Ovalbumin;Ovotransferrin;Serum albumin

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05453170/
• ペルオキシダーゼのX線結晶構造解析と機能に関する研究

  Grant-in-Aid for General Scientific Research (C)

  Kyoto University

  森田 雄平

  From 01 Apr. 1988, To 31 Mar. 1989, Project Closed

  ペルオキシダーゼ;タンパク質X線構造解析;ミエロペルオキシダーゼ;ヘムタンパク質

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63560084/
• Studies on the Structure and Function of Myeloperoxidase from Normal Human Leukocytes

  Grant-in-Aid for General Scientific Research (B)

  Kyoto University

  Yuhei MORITA

  From 01 Apr. 1986, To 31 Mar. 1988, Project Closed

  ミエロペルオキシダーゼの構造;カタラーゼ活性;ヒト白血球, Myeloperoxidase, structure of;Catalase activity

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61470128/
• Elucidation of enzyme function by X-ray crystal structure at room temperature.

  Grant-in-Aid for Scientific Research (C)

  Basic Section 38030:Applied biochemistry-related

  Kyoto University

  三上 文三

  From 01 Apr. 2022, To 31 Mar. 2025, Granted

  非凍結X線結晶構造解析;常温Ｘ線結晶構造解析;酵素のpHによる構造変化;酵素反応機構;β-アミラーゼ;結晶内酵素反応;X線結晶構造解析;非凍結結晶;構造生物学

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K05441/

External funds: others

• Studies on the catalytic mechanism of soybean β-amylase by X-ray crystallography
• Studies on the three-dimensional structure of food proteins
• Studies on the three-dimensional structures of food related enzymes

Education

Teaching subject(s)

• From Apr. 2011, To Mar. 2012

  応用生命科学入門I

  Spring, 農学部
• From Apr. 2011, To Mar. 2012

  構造生物学

  Fall, 農学部
• From Apr. 2011, To Mar. 2012

  生物理化学実験

  Spring, 農学部
• From Apr. 2011, To Mar. 2012

  Applied Life Sciences

  Fall, 全学共通科目
• From Apr. 2011, To Mar. 2012

  応用構造生物学専攻実験

  農学研究科
• From Apr. 2011, To Mar. 2012

  応用構造生物学専攻演習

  農学研究科
• From Apr. 2011, To Mar. 2012

  応用構造生物学

  農学研究科
• From Apr. 2012, To Mar. 2013

  Applied Life Sciences

  Fall, 全学共通科目
• From Apr. 2012, To Mar. 2013

  Introduction to Applied Life Science I

  Spring, 農学部
• From Apr. 2012, To Mar. 2013

  Structural Biology

  Fall, 農学部
• From Apr. 2012, To Mar. 2013

  Laboratory Course in Biophysical Chemistry

  Spring, 農学部
• From Apr. 2013, To Mar. 2014

  Introduction to Applied Life Science I

  Spring, 農学部
• From Apr. 2013, To Mar. 2014

  Structural Biology

  Fall, 農学部
• From Apr. 2013, To Mar. 2014

  Laboratory Course in Biophysical Chemistry

  Spring, 農学部
• From Apr. 2013, To Mar. 2014

  Applied Life Sciences I

  Spring, 農学研究科
• From Apr. 2013, To Mar. 2014

  Applied Structural Biology

  Spring, 農学研究科
• From Apr. 2013, To Mar. 2014

  Applied Structural Biology Seminar

  Year-long, 農学研究科
• From Apr. 2013, To Mar. 2014

  Experimental Course of Applied Structural Biology

  Year-long, 農学研究科
• From Apr. 2013, To Mar. 2014

  Applied Life Sciences

  Fall, 全学共通科目
• From Apr. 2014, To Mar. 2015

  Introduction to Applied Life Science I

  Spring, 農学部
• From Apr. 2014, To Mar. 2015

  Structural Biology

  Fall, 農学部
• From Apr. 2014, To Mar. 2015

  Scientific English

  Spring, 農学部
• From Apr. 2014, To Mar. 2015

  Laboratory Course in Biophysical Chemistry

  Spring, 農学部
• From Apr. 2014, To Mar. 2015

  Applied Life Sciences I

  Spring, 農学研究科
• From Apr. 2014, To Mar. 2015

  Applied Structural Biology

  Spring, 農学研究科
• From Apr. 2014, To Mar. 2015

  Applied Structural Biology Seminar

  Year-long, 農学研究科
• From Apr. 2014, To Mar. 2015

  Experimental Course of Applied Structural Biology

  Year-long, 農学研究科
• From Apr. 2014, To Mar. 2015

  Applied Life Sciences

  Fall, 全学共通科目
• From Apr. 2014, To Mar. 2015

  Looking at and capture the protein structure

  Spring, 全学共通科目
• From Apr. 2015, To Mar. 2016

  Experimental Course of Applied Structural Biology1

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Experimental Course of Applied Structural Biology

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Applied Structural Biology Seminar1

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Applied Structural Biology Seminar

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Applied Structural Biology

  Spring, 農学研究科
• From Apr. 2015, To Mar. 2016

  Applied Life Sciences I

  Spring, 農学研究科
• From Apr. 2015, To Mar. 2016

  Introduction to Applied Life Science I

  Spring, 農学部
• From Apr. 2015, To Mar. 2016

  Structural Biology

  Fall, 農学部
• From Apr. 2015, To Mar. 2016

  Laboratory Course in Biophysical Chemistry

  Spring, 農学部
• From Apr. 2016, To Mar. 2017

  ILAS Seminar

  Spring, 全学共通科目
• From Apr. 2016, To Mar. 2017

  Experimental Course of Applied Structural Biology1

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Experimental Course of Applied Structural Biology2

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Experimental Course of Applied Structural Biology

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Applied Structural Biology Seminar1

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Applied Structural Biology Seminar2

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Applied Structural Biology Seminar

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Applied Structural Biology

  Spring, 農学研究科
• From Apr. 2016, To Mar. 2017

  Applied Life Sciences I

  Spring, 農学研究科
• From Apr. 2016, To Mar. 2017

  Introduction to Applied Life Science I

  Spring, 農学部
• From Apr. 2016, To Mar. 2017

  Structural Biology

  Fall, 農学部
• From Apr. 2016, To Mar. 2017

  Laboratory Course in Biophysical Chemistry

  Spring, 農学部
• From Apr. 2017, To Mar. 2018

  Experimental Course of Applied Structural Biology1

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Experimental Course of Applied Structural Biology2

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Experimental Course of Applied Structural Biology

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Applied Structural Biology Seminar1

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Applied Structural Biology Seminar2

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Applied Structural Biology Seminar

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Applied Structural Biology

  Spring, 農学研究科
• From Apr. 2017, To Mar. 2018

  Applied Life Sciences I

  Spring, 農学研究科
• From Apr. 2017, To Mar. 2018

  Introduction to Applied Life Science I

  Spring, 農学部
• From Apr. 2017, To Mar. 2018

  Structural Biology

  Fall, 農学部
• From Apr. 2017, To Mar. 2018

  Laboratory Course in Biophysical Chemistry

  Spring, 農学部
• From Apr. 2018, To Mar. 2019

  Experimental Course of Applied Structural Biology1

  Year-long, 農学研究科
• From Apr. 2018, To Mar. 2019

  Experimental Course of Applied Structural Biology2

  Year-long, 農学研究科
• From Apr. 2018, To Mar. 2019

  Applied Structural Biology Seminar1

  Year-long, 農学研究科
• From Apr. 2018, To Mar. 2019

  Applied Structural Biology Seminar2

  Year-long, 農学研究科
• From Apr. 2018, To Mar. 2019

  Applied Structural Biology

  Spring, 農学研究科
• From Apr. 2018, To Mar. 2019

  Applied Life Sciences I

  Spring, 全学共通科目
• From Apr. 2018, To Mar. 2019

  Applied Life Sciences I

  Spring, 農学研究科
• From Apr. 2018, To Mar. 2019

  Introduction to Applied Life Science I

  Spring, 農学部
• From Apr. 2018, To Mar. 2019

  Structural Biology

  Fall, 農学部
• From Apr. 2018, To Mar. 2019

  Laboratory Course in Biophysical Chemistry

  Spring, 農学部

Part-time lecturer

• From 2010, To 2010

  同志社大学, 生命医学部
• From 2009, To 2009

  同志社大学, 生命医学部
• From 2009, To 2009

  神戸大学, 農学部
• From 2009, To 2009

  島根大学, 農学部
• From 2008, To 2008

  近畿大学, 農学部

Administration

School management (title, position)

• From 01 Apr. 2012, To 31 Mar. 2014

  原子力研究整備委員会 委員
• From 01 Apr. 2014, To 31 Mar. 2016

  原子力研究整備委員会 委員
• From 19 Jun. 2009, To 18 Jun. 2011

  宇治地区学術情報・通信整備運用委員会 委員
• From 19 Jun. 2011, To 31 Mar. 2015

  宇治地区学術情報・通信整備運用委員会 委員
• From 01 Apr. 2010, To 31 Mar. 2012

  宇治地区施設整備・将来計画委員会 委員
• From 01 Apr. 2012, To 31 Mar. 2014

  宇治地区施設整備・将来計画委員会 委員
• From 01 Apr. 2014, To 31 Mar. 2016

  宇治地区施設整備・将来計画委員会 委員
• From 01 Apr. 2012, To 31 Mar. 2013

  附属図書館運営委員会 委員
• From 01 Apr. 2011, To 31 Mar. 2013

  附属図書館協議会 委員
• From 01 Apr. 2011, To 31 Mar. 2012

  附属図書館選書小委員会 委員
• From 01 Apr. 2016, To 31 Mar. 2018

  原子力研究整備委員会 委員
• From 01 Jan. 2016, To 31 Dec. 2016

  宇治キャンパス公開実行委員会 委員
• From 01 Apr. 2016, To 31 Mar. 2018

  宇治地区施設整備・将来計画委員会 委員
• From 01 Apr. 2016, To 31 Mar. 2018

  京都大学化学研究所「化学関連分野の深化・連携を基軸とする先端・学際研究拠点」平成28～29年度専門小委員会

Faculty management (title, position)

• From 01 Apr. 2012, To 31 Mar. 2014

  宇治地区施設整備・将来計画委員会
• From 01 Apr. 2014, To 31 Mar. 2016

  宇治地区施設整備・将来計画委員会
• From 01 Apr. 2013, To 31 Mar. 2017

  情報セキュリティ・システム委員会　委員
• From 01 Apr. 2015, To 31 Mar. 2016

  応用生命科学科長
• From 01 Apr. 2015, To 31 Mar. 2016

  評価委員会　委員
• From 01 Apr. 2005

  放射線障害防止委員会　委員