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Yamashiro, Sawako

Graduate School of Biostudies, Division of Systemic Life Science Senior Lecturer/ Junior Associate Professor

Yamashiro, Sawako
list
    Last Updated :2022/06/24

    Basic Information

    Faculty

    • 医学部

    Email Address

    • Email Address

      yamashiro.sawako.5ckyoto-u.ac.jp

    Professional Memberships

    • American Society for Cell Biology
    • 日本薬理学会
    • 日本分子生物学会
    • 日本細胞生物学会

    Academic Degree

    • 修士(理工学)(上智大学)
    • 博士(学術)(東京大学)

    ID,URL

    researchmap URL

    list
      Last Updated :2022/06/24

      Research

      Research Topics, Overview of the research

      • Research Topics

        蛍光単分子イメージングによるアクチン依存的細胞内力の動態と役割の解明

      Research Areas

      • Life sciences, Cell biology, アクチン細胞骨格、蛍光1分子顕微鏡、メカノバイオロジー

      Papers

      • A mechanism with severing near barbed ends andannealing explains structure and dynamics of dendriticactin networks.
        Danielle Holz; Aaron R Hall; Eiji Usukura; Sawako Yamashiro; Naoki Watanabe; Dimitrios Vavylonis
        eLife, 07 Jun. 2022
      • Lamellipodium tip actin barbed ends serve as a force sensor.
        Koseki K; Taniguchi D; Yamashiro S; Mizuno H; Vavylonis D; Watanabe N
        Genes to cells : devoted to molecular & cellular mechanisms, Nov. 2019, Peer-reviewed
      • Quantitative high-precision imaging of myosin-dependent filamentous actin dynamics.
        Yamashiro S; Watanabe N
        Journal of muscle research and cell motility, Jul. 2019, Peer-reviewed, Invited
      • Convection-Induced Biased Distribution of Actin Probes in Live Cells.
        Yamashiro S; Taniguchi D; Tanaka S; Kiuchi T; Vavylonis D; Watanabe N
        Biophysical journal, Jan. 2019, Peer-reviewed
      • Mechanostress resistance involving formin homology proteins: G- and F-actin homeostasis-driven filament nucleation and helical polymerization-mediated actin polymer stabilization.
        Watanabe N; Tohyama K; Yamashiro S
        Biochemical and biophysical research communications, Nov. 2018, Peer-reviewed
      • Helical rotation of the diaphanous-related formin mDia1 generates actin filaments resistant to cofilin
        Hiroaki Mizuno; Kotaro Tanaka; Sawako Yamashiro; Akihiro Narita; Naoki Watanabe
        Proceedings of the National Academy of Sciences of the United States of America, 29 May 2018, Peer-reviewed
      • Myosin-dependent actin stabilization as revealed by single-molecule imaging of actin turnover.
        Yamashiro S; Tanaka S; McMillen LM; Taniguchi D; Vavylonis D; Watanabe N
        Molecular biology of the cell, May 2018, Peer-reviewed
      • Cell protrusion and retraction driven by fluctuations in actin polymerization: A two-dimensional model
        Gillian L. Ryan; Danielle Holz; Sawako Yamashiro; Daisuke Taniguchi; Naoki Watanabe; Dimitrios Vavylonis
        CYTOSKELETON, Dec. 2017, Peer-reviewed
      • An Infrared Actin Probe for Deep-Cell Electroporation-Based Single-Molecule Speckle (eSiMS) Microscopy
        Sawako Yamashiro; Naoki Watanabe
        SENSORS, Jul. 2017, Peer-reviewed
      • Overview of Single-Molecule Speckle (SiMS) Microscopy and Its Electroporation-Based Version with Efficient Labeling and Improved Spatiotemporal Resolution
        Sawako Yamashiro; Naoki Watanabe
        SENSORS, Jul. 2017, Peer-reviewed
      • UNC-87 isoforms, Caenorhabditis elegans calponin-related proteins, interact with both actin and myosin and regulate actomyosin contractility
        Kanako Ono; Takashi Obinata; Sawako Yamashiro; Zhongmei Liu; Shoichiro Ono
        MOLECULAR BIOLOGY OF THE CELL, May 2015, Peer-reviewed
      • An easy-to-use single-molecule speckle microscopy enabling nanometer-scale flow and wide-range lifetime measurement of cellular actin filaments
        Sawako Yamashiro; Hiroaki Mizuno; Naoki Watanabe
        BIOPHYSICAL METHODS IN CELL BIOLOGY, 2015, Peer-reviewed
      • A new link between the retrograde actin flow and focal adhesions
        Sawako Yamashiro; Naoki Watanabe
        JOURNAL OF BIOCHEMISTRY, Nov. 2014, Peer-reviewed
      • Functional Effects of Mutations in the Tropomyosin-Binding Sites of Tropomodulin1 and Tropomodulin3
        Raymond A. Lewis; Sawako Yamashiro; David S. Gokhin; Velia M. Fowler
        CYTOSKELETON, Jul. 2014, Peer-reviewed
      • New single-molecule speckle microscopy reveals modification of the retrograde actin flow by focal adhesions at nanometer scales
        Sawako Yamashiro; Hiroaki Mizuno; Matthew B. Smith; Gillian L. Ryan; Tai Kiuchi; Dimitrios Vavylonis; Naoki Watanabe
        MOLECULAR BIOLOGY OF THE CELL, Apr. 2014, Peer-reviewed
      • Differential Actin-regulatory Activities of Tropomodulin1 and Tropomodulin3 with Diverse Tropomyosin and Actin Isoforms*
        Sawako Yamashiro; David S. Gokhin; Zhenhua Sui; Sarah E. Bergeron; Peter A. Rubenstein; Velia M. Fowler
        JOURNAL OF BIOLOGICAL CHEMISTRY, Apr. 2014, Peer-reviewed
      • Molecular viewing of actin polymerizing actions and beyond: combination analysis of single-molecule speckle microscopy with modeling, FRAP and s-FDAP (sequential fluorescence decay after photoactivation).
        Watanabe N; Yamashiro S; Vavylonis D; Kiuchi T
        Development, growth & differentiation, May 2013, Peer-reviewed, Invited
      • Tropomodulin Protects alpha-Catenin-Dependent Junctional-Actin Networks under Stress during Epithelial Morphogenesis
        Elisabeth A. Cox-Paulson; Elise Walck-Shannon; Allison M. Lynch; Sawako Yamashiro; Ronen Zaidel-Bar; Celeste C. Eno; Shoichiro Ono; Jeff Hardin
        CURRENT BIOLOGY, Aug. 2012, Peer-reviewed
      • Tropomodulins: Pointed-end capping proteins that regulate actin filament architecture in diverse cell types
        Sawako Yamashiro; David S. Gokhin; Sumiko Kimura; Roberta B. Nowak; Velia M. Fowler
        CYTOSKELETON, Jun. 2012
      • Total Synthesis and Biological Evaluation of Monorhizopodin and 16-epi-Monorhizopodin
        K. C. Nicolaou; Xuefeng Jiang; Peter J. Lindsay-Scott; Andrei Corbu; Sawako Yamashiro; Andrea Bacconi; Velia M. Fowler
        ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, Peer-reviewed
      • Mammalian Tropomodulins Nucleate Actin Polymerization via Their Actin Monomer Binding and Filament Pointed End-capping Activities
        Sawako Yamashiro; Kaye D. Speicher; David W. Speicher; Velia M. Fowler
        JOURNAL OF BIOLOGICAL CHEMISTRY, Oct. 2010, Peer-reviewed
      • Sarcomeric actin organization is synergistically promoted by tropomodulin, ADF/cofilin, AIP1 and profilin in C-elegans
        Sawako Yamashiro; Elisabeth A. Cox; David L. Baillie; Jeff D. Hardin; Shoichiro Ono
        JOURNAL OF CELL SCIENCE, Dec. 2008, Peer-reviewed
      • Caenorhabditis elegans gelsolin-like protein 1 is a novel actin filament-severing protein with four gelsolin-like repeats
        Tuula Klaavuniemi; Sawako Yamashiro; Shoichiro Ono
        JOURNAL OF BIOLOGICAL CHEMISTRY, Sep. 2008, Peer-reviewed
      • Essential role of ADF/cofilin for assembly of contractile actin networks in the C-elegans somatic gonad
        Kanako Ono; Sawako Yamashiro; Shoichiro Ono
        JOURNAL OF CELL SCIENCE, Aug. 2008, Peer-reviewed
      • UNC-87, a calponin-related protein in C. elegans, antagonizes ADF/cofilin-mediated actin filament dynamics
        Sawako Yamashiro; Mario Gimona; Shoichiro Ono
        JOURNAL OF CELL SCIENCE, Sep. 2007, Peer-reviewed
      • IQGAP2 is required for the cadherin-mediated cell-to-cell adhesion in Xenopus laevis embryos
        Sawako Yamashiro; Hiroshi Abe; Issei Mabuchi
        DEVELOPMENTAL BIOLOGY, Aug. 2007, Peer-reviewed
      • Enhancement of actin-depolymerizing factor/cofilin-dependent actin disassembly by actin-interacting protein 1 is required for organized actin filament assembly in the Caenorhabditis elegans body wall muscle
        K Mohri; K Ono; R Yu; S Yamashiro; S Ono
        MOLECULAR BIOLOGY OF THE CELL, May 2006, Peer-reviewed
      • The two Caenorhabditis elegans actin-depolymerizing factor/cofilin proteins differently enhance actin filament severing and depolymerization
        S Yamashiro; K Mohri; S Ono
        BIOCHEMISTRY, Nov. 2005, Peer-reviewed
      • Localization of two IQGAPs in cultured cells and early embryos of Xenopus laevis
        S Yamashiro; T Noguchi; Mabuchi, I
        CELL MOTILITY AND THE CYTOSKELETON, May 2003, Peer-reviewed
      • Microtuble organization in Xenopus eggs during the first cleavage and its role in cytokinesis
        M Takayama; T Noguchi; S Yamashiro; Mabuchi, I
        CELL STRUCTURE AND FUNCTION, Aug. 2002

      Misc.

      • 細胞内蛍光単分子イメージングによるミオシン張力依存的なアクチン線維安定化の証明
        山城佐和子; 渡邊直樹
        生体の科学, Jun. 2021, Invited, Lead author
      • アクチンネットワークを駆け巡る力学作用の分子可視化
        渡邊直樹; 山城佐和子
        生化学, Feb. 2020, Invited, Last author
      • 蛍光単分子イメージングが解き明かすミオシン張力によるアクチン線維安定化
        山城佐和子; 渡邊直樹
        月刊メディカル・サイエンス・ダイジェスト, 30 Jun. 2019
      • 生細胞内でのアクチンフィラメント配向の可視化
        通山 潔; 山城 佐和子; 渡邊 直樹
        日本生化学会大会プログラム・講演要旨集, Sep. 2018
      • 分子から個体まで神経系を見る 分子の窓を通して覗いた細胞の構造としくみ
        渡邊 直樹; 木内 泰; 山城 佐和子
        バイオイメージング, Aug. 2016
      • ナノスケール単分子スペックル解析により明らかにする細胞仮足が「つかみ取る」しくみ
        山城佐和子; 山城佐和子; 田中聡一郎; 渡邊直樹; 渡邊直樹
        日本細胞生物学会大会(Web), 2016
      • 【細胞シグナル操作法】機能からみたシグナル操作法 細胞の動態 細胞骨格(アクチン系)
        山城 佐和子; 渡邊 直樹
        生体の科学, 15 Oct. 2015
      • 【Molecular Imaging 2014 分子イメージングはどこまで進んだか】 分子イメージングの最新動向 光イメージングの最新動向 細胞内蛍光単分子イメージングが解き明かす生命の動的なしくみ
        渡邊 直樹; 山城 佐和子; 水野 裕昭
        INNERVISION, Jun. 2014
      • Update Review アクチン研究の最新動向 構造から調節、恒常性、可視化、モデリングまで
        山城 佐和子; 圓岡 真宏; 水野 裕昭; 渡邊 直樹
        実験医学, Nov. 2012
      • フォルミンmDia1の螺旋回転を介したアクチン脱重合抵抗性
        水野裕昭; 山城佐和子; 渡邊直樹
        日本分子生物学会年会プログラム・要旨集(Web), 2012
      • Inhibition of ADF/cofilin binding to actin filaments by UNC-87, a C-elegans calponin-repeat protein
        S Yamashiro; JH Woo; M Gimona; S Ono
        MOLECULAR BIOLOGY OF THE CELL, Nov. 2004
      • Profilin contributes to cleavage furrow formation in Xenopus eggs
        OKazuhiro Yamada; Sawako Yamashiro; Issei Mabuchi
        CELL STRUCTURE AND FUNCTION, May 2004
      • Analysis of localization and function of two isoforms of Xenopus IQGAP in early embryos and cultured cells
        S Yamashiro; T Noguchi; Mabuchi, I
        MOLECULAR BIOLOGY OF THE CELL, Nov. 2002

      Presentations

      • Coupling between focal adhesion molecules and retrograde actin flow visualized by quantitative live-cell single-molecule imaging
        山城佐和子; 劉穎; 渡邊直樹
        第44回日本分子生物学会年会, 01 Dec. 2021
      • 高精度単分子イメージングによるアクチン繊維流動ー接着斑連関の可視化解明
        山城佐和子; 劉穎; 渡邊直樹
        第73回日本細胞生物学会, 02 Jul. 2021
      • New insights into the retrograde F-actin flow-associated motions of focal adhesion molecules visualized by single-molecule speckle (SiMS) microscopy
        Sawako Yamashiro
        The 43rd Annual Meeting of the Molecular Biology Society of Japan, 04 Dec. 2020, Invited
      • 単分子イメージングによるアクチン線維流動ー接着斑分子連結機構の可視化解明
        山城佐和子; 劉穎; 渡邊直樹
        第19回日本蛋白質科学会・第71回日本細胞生物学会合同年次大会, 25 Jun. 2019
      • Myosin-dependent actin stabilization as revealed by single-molecule speckle (SiMS) analysis of actin turnover.
        Sawako Yamashiro
        The 16th International Membrane Research Forum, 20 Mar. 2019, Invited
      • Retrograde flow-induced biased distribution of actin probes in live cells.
        山城佐和子
        American Society for Cell Biology / EMBO 2018 meeting, 09 Dec. 2018

      Books and Other Publications

      • ラング・デール薬理学
        山城 佐和子, Joint translation
        丸善出版, Dec. 2018, Not refereed
      • 生体の科学 特集:細胞シグナル操作法、第66巻 第5号
        山城 佐和子, Contributor, 細胞骨格(アクチン系)pp504-505
        医学書院, 2015, Not refereed
      • 実験医学 第30巻 第18号
        山城 佐和子, Contributor, アクチン研究の最新動向-構造から調節、恒常性、可視化、モデリングまで: Update Review pp2998-3005
        羊土社, 2012, Not refereed

      External funds: Kakenhi

      • 高精度単分子イメージングによるアクチンダイナミクス-接着斑連関の生理的意義の解明
        Grant-in-Aid for Scientific Research (C)
        Basic Section 44010:Cell biology-related
        Kyoto University
        山城 佐和子
        From 01 Apr. 2021, To 31 Mar. 2024, Granted
        細胞接着;接着斑;アクチン細胞骨格;細胞内蛍光1分子イメージング;アクチン線維流動
      • Visualization of microenvironment sensing mechanism by fluorescence single-molecule imaging
        Grant-in-Aid for Transformative Research Areas (A)
        Transformative Research Areas, Section (III)
        Kyoto University
        山城 佐和子
        From 10 Sep. 2021, To 31 Mar. 2023, Granted
        メカノバイオロジー;剪断応力;細胞膜タンパク質;蛍光1分子顕微鏡;微小環境
      • 高解像度分子可視化による力学的情報メモリの定量的解析
        Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
        Complex systems
        Kyoto University
        山城 佐和子
        From 01 Apr. 2020, To 31 Mar. 2022, Granted
        細胞内蛍光1分子イメージング;メカノセンシング;ずり応力;細胞膜タンパク質;数理モデル解析;メカノバイオロジー;蛍光1分子顕微鏡;細胞膜;接着分子;受容体;GPIアンカー型タンパク質;1分子イメージング;アクチン細胞骨格
      • Quantitative high-precision live-cell single-molecule imaging of focal adhesion molecules
        Grant-in-Aid for Scientific Research (C)
        Basic Section 44010:Cell biology-related
        Kyoto University
        Sawako Yamashiro
        From 01 Apr. 2018, To 31 Mar. 2021, Project Closed
        1分子イメージング;アクチン;細胞内流動;細胞接着;定量的イメージング;数理モデル;接着斑;細胞運動;細胞内蛍光1分子顕微鏡;定量ライブイメージング;数理モデル解析;ミオシン;一分子イメージング;ライブイメージング;アクチン細胞骨格;メカノセンサー分子
      • The role of actomyosin contraction on actin filament stability revealed by using new easy-to-use Single-Molecule Speckle (eSiMS) microscopy.
        Grant-in-Aid for Scientific Research (C)
        Kyoto University
        Yamashiro Sawako
        From 01 Apr. 2015, To 31 Mar. 2018, Project Closed
        一分子イメーシング;定量的イメージング;メカノセンシング;アクチン;ミオシン;一分子イメージング;接着斑;細胞運動
      • 革新的イメージングによる上皮細胞間コミュニケーションの異常における力の役割
        Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
        Biological Sciences
        Kyoto University
        山城 佐和子
        From 01 Apr. 2014, To 31 Mar. 2016, Project Closed
        蛍光単分子イメージング;メカノセンシング;アクチン;接着斑;細胞外基質;顕微鏡技術;一分子イメージング;アクチン細胞骨格;上皮細胞;細胞接着
      • Quantitative analysis of the retrograde actin flow by a new single-molecule speckle microscopy
        Grant-in-Aid for Young Scientists (B)
        Tohoku University
        Sawako YAMASHIRO
        From 01 Apr. 2012, To 31 Mar. 2014, Project Closed
        単分子スペックル法;一分子イメージング;アクチン;細胞運動;計測生物学;国際情報交換
      • 上皮細胞ラテラル領域におけるアクチン繊維流動”力”の機能解明
        Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
        Biological Sciences
        Tohoku University
        山城 佐和子
        From 01 Apr. 2012, To 31 Mar. 2014, Project Closed
        一分子イメージング;アクチン細胞骨格;細胞運動;細胞接着;国際情報交換(アメリカ)
      • 高解像度分子可視化による力学的方向情報伝達機構の解明
        Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
        Complex systems
        Kyoto University
        山城 佐和子
        From 01 Apr. 2022, To 31 Mar. 2024, Granted
        shear stress;蛍光1分子顕微鏡;細胞膜分子;メカノセンス;アクチン細胞骨格
      list
        Last Updated :2022/06/24

        Education

        Teaching subject(s)

        • From 01 Apr. 2022, To 31 Mar. 2023
          Experimental Course and Seminar D
          2009, Spring, Graduate School of Biostudies, 5
        • From 01 Apr. 2022, To 31 Mar. 2023
          Global Frontier in Life Science B
          1033, Fall, Graduate School of Biostudies, 1
        • From 01 Apr. 2022, To 31 Mar. 2023
          Experimental Course and Seminar B
          2007, Fall, Graduate School of Biostudies, 5
        • From 01 Apr. 2022, To 31 Mar. 2023
          Experimental Course and Seminar D
          2009, Fall, Graduate School of Biostudies, 5
        • From 01 Apr. 2022, To 31 Mar. 2023
          Advanced Experiments in Systemic Life Sciences
          5013, Fall, Graduate School of Biostudies, 4
        • From 01 Apr. 2022, To 31 Mar. 2023
          Advanced Course in Immunology and Human Disorders
          1031, Spring, Graduate School of Biostudies, 1
        • From 01 Apr. 2022, To 31 Mar. 2023
          Experimental Course and Seminar C
          2008, Fall, Graduate School of Biostudies, 5
        • From 01 Apr. 2022, To 31 Mar. 2023
          Experimental Course and Seminar C
          2008, Spring, Graduate School of Biostudies, 5
        • From 01 Apr. 2022, To 31 Mar. 2023
          Experimental Course and Seminar A
          2006, Fall, Graduate School of Biostudies, 5
        • From 01 Apr. 2022, To 31 Mar. 2023
          Experimental Course and Seminar A
          2006, Spring, Graduate School of Biostudies, 5
        • From 01 Apr. 2022, To 31 Mar. 2023
          Experimental Course and Seminar B
          2007, Spring, Graduate School of Biostudies, 5
        • From 01 Apr. 2022, To 31 Mar. 2023
          Advanced Experiments in Systemic Life Sciences
          5013, Spring, Graduate School of Biostudies, 4
        • From Apr. 2019, To Mar. 2020
          Advanced Course in Immunology and Human Disorders
          Spring, 生命科学研究科
        • From Apr. 2020, To Mar. 2021
          Global Frontier in Life Science B
          Fall, 生命科学研究科
        • From Apr. 2020, To Mar. 2021
          Advanced Course in Immunology and Human Disorders
          Fall, 生命科学研究科
        • From Apr. 2021, To Mar. 2022
          Global Frontier in Life Science B
          Fall, 生命科学研究科
        • From Apr. 2021, To Mar. 2022
          Experimental Course and Seminar
          Spring, 生命科学研究科
        • From Apr. 2021, To Mar. 2022
          Experimental Course and Seminar
          Spring, 生命科学研究科
        • From Apr. 2021, To Mar. 2022
          Experimental Course and Seminar
          Spring, 生命科学研究科
        • From Apr. 2021, To Mar. 2022
          Experimental Course and Seminar
          Spring, 生命科学研究科
        • From Apr. 2021, To Mar. 2022
          Experimental Course and Seminar
          Fall, 生命科学研究科
        • From Apr. 2021, To Mar. 2022
          Experimental Course and Seminar
          Fall, 生命科学研究科
        • From Apr. 2021, To Mar. 2022
          Experimental Course and Seminar
          Fall, 生命科学研究科
        • From Apr. 2021, To Mar. 2022
          Experimental Course and Seminar
          Fall, 生命科学研究科
        • From Apr. 2021, To Mar. 2022
          Advanced Experiments in Systemic Life Sciences
          Spring, 生命科学研究科
        • From Apr. 2021, To Mar. 2022
          Advanced Experiments in Systemic Life Sciences
          Fall, 生命科学研究科
        • From Apr. 2021, To Mar. 2022
          Advanced Course in Immunology and Human Disorders
          Spring, 生命科学研究科

        Student achievements: Awards

        • 新学術領域「生命の情報物理学」第1回領域研究賞
          野村実里, 新学術領域「情報物理学でひもとく生命の秩序と設計原理」, 15 Feb. 2022
        list
          Last Updated :2022/06/24

          Administration

          Faculty management (title, position)

          • From 01 Apr. 2022
            情報発信・ホームページ委員会
          • From 01 Apr. 2019
            よろず相談窓口委員会 (ハラスメント相談窓口委員会)
          • From 01 Apr. 2021
            男女共同参画推進アクションプラン推進委員会 委員

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