Basic Information

Professional Memberships

• 高分子学会
• 日本ペプチド学会
• 日本化学会

Research History

• From Jan. 2022, To Present

  Kyoto University, Graduate School of Engineering, 特定准教授
• From Nov. 2017, To Dec. 2021

  Kyoto University, Graduate School of Engineering, 特定助教
• From May 2017, To Oct. 2017

  Kyoto University, Graduate School of Engineering, 博士研究員
• From Apr. 2007, To Mar. 2008

  The University of Tokyo, Institute of Industrial Science, Assistant Professor
• From Apr. 2001, To Sep. 2002

  Kyoto University, Institute of Advanced Energy
• From Oct. 2002, To Mar. 2007

  東京大学生産技術研究所 助手
• From Apr. 2008, To Apr. 2017

  Tohoku University, Institute of Multidisciplinary Research for Advanced Materials, Assistant Professor

ID,URL

• J-Global ID

  200901021890000113

researchmap URL

• https://researchmap.jp/read0213310

Research

Research Topics, Overview of the research

• Research Topics

  Chemical labeling of proteins

• Overview of the research

  Methods for chemical labeling of endogenous proteins in live cells and animals are explored.

Research Interests

• ペプチド化学
• タンパク質
• Protrin and peptide chemistry

Research Areas

• Nanotechnology/Materials, Molecular biochemistry

Papers

• Bioorthogonal chemical labeling of endogenous neurotransmitter receptors in living mouse brains

  Hiroshi Nonaka; Seiji Sakamoto; Kazuki Shiraiwa; Mamoru Ishikawa; Tomonori Tamura; Kyohei Okuno; Takumi Kondo; Shigeki Kiyonaka; Etsuo A. Susaki; Chika Shimizu; Hiroki R. Ueda; Wataru Kakegawa; Itaru Arai; Michisuke Yuzaki; Itaru Hamachi

  Proceedings of the National Academy of Sciences, 31 Jan. 2024

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• Coronaridine congeners induce sedative and anxiolytic-like activity in naïve and stressed/anxious mice by allosteric mechanisms involving increased GABAA receptor affinity for GABA

  Hugo R. Arias; Philippe De Deurwaerdère; Petra Scholze; Seiji Sakamoto; Itaru Hamachi; Giuseppe Di Giovanni; Abdeslam Chagraoui

  European Journal of Pharmacology, Aug. 2023

  
• Chemical, Pharmacological, and Structural Characterization of Novel Acrylamide-Derived Modulators of the GABA_AReceptor

  Hugo R. Arias; Spencer R. Pierce; Allison L. Germann; Sophia Q. Xu; Marcelo O. Ortells; Seiji Sakamoto; Dina Manetti; Maria Novella Romanelli; Itaru Hamachi; Gustav Akk

  Molecular Pharmacology, 14 Jun. 2023

  
• Revisiting PFA-mediated tissue fixation chemistry: FixEL enables trapping of small molecules in the brain to visualize their distribution changes

  Hiroshi Nonaka; Takeharu Mino; Seiji Sakamoto; Jae Hoon Oh; Yu Watanabe; Mamoru Ishikawa; Akihiro Tsushima; Kazuma Amaike; Shigeki Kiyonaka; Tomonori Tamura; A. Radu Aricescu; Wataru Kakegawa; Eriko Miura; Michisuke Yuzaki; Itaru Hamachi

  Chem, Feb. 2023

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• Modulation of the mammalian GABA_A receptor by type I and type II positive allosteric modulators of the α7 nicotinic acetylcholine receptor

  Hugo R. Arias; Allison L. Germann; Spencer R. Pierce; Seiji Sakamoto; Marcelo O. Ortells; Itaru Hamachi; Gustav Akk

  British Journal of Pharmacology, 27 Sep. 2022

  
• Structure-dependent recruitment and diffusion of guest proteins in liquid droplets of FUS

  Kiyoto Kamagata; Nanako Iwaki; Saori Kanbayashi; Trishit Banerjee; Rika Chiba; Virginie Gaudon; Bertrand Castaing; Seiji Sakomoto

  Scientific Reports, 02 May 2022

  
• Molecular principles of recruitment and dynamics of guest proteins in liquid droplets

  Kiyoto Kamagata; Nanako Iwaki; Milan Kumar Hazra; Saori Kanbayashi; Trishit Banerjee; Rika Chiba; Seiji Sakomoto; Virginie Gaudon; Bertrand Castaing; Hiroto Takahashi; Michiko Kimura; Hiroyuki Oikawa; Satoshi Takahashi; Yaakov Levy

  Scientific Reports, 29 Sep. 2021

  
• Screening of protein-ligand interactions under crude conditions by native mass spectrometry

  Kotaro Takano; Shunsuke Arai; Seiji Sakamoto; Hiroshi Ushijima; Takahisa Ikegami; Kazumi Saikusa; Tsuyoshi Konuma; Itaru Hamachi; Satoko Akashi

  Analytical and Bioanalytical Chemistry, 23 Apr. 2020

  
• Construction of a Fluorescent Screening System of Allosteric Modulators for the GABA_A Receptor Using a Turn-On Probe

  Seiji Sakamoto; Kei Yamaura; Tomohiro Numata; Fumio Harada; Kazuma Amaike; Ryuji Inoue; Shigeki Kiyonaka; Itaru Hamachi

  ACS Central Science, 16 Aug. 2019

  
• Transient Circular Dichroism Measurement of the Excited Triplet State of Pristine Hexahelicene in Solution at Room Temperature

  Makoto Kuronuma; Takehito Sato; Yasuyuki Araki; Tadashi Mori; Seiji Sakamoto; Yoshihisa Inoue; Osamu Ito; Takehiko Wada

  Chemistry Letters, 05 Apr. 2019

  
• N-Benzoyl-Protected Peptide Nucleic Acid (PNA) Monomers Expand the Range of Nucleobases Available for PNA-DNA Chimera

  Masahito Inagaki; Ryohei Uematsu; Tatsuya Mizutani; Daisuke Unabara; Yasuyuki Araki; Seiji Sakamoto; Hiromu Kashida; Masaki Nishijima; Hiroyuki Asanuma; Yoshihisa Inoue; Takehiko Wada

  Chemistry Letters, Apr. 2019, Peer-reviewed

  
• Ligand-Directed Chemistry of AMPA Receptors Confers Live-Cell Fluorescent Biosensors

  Shigeki Kiyonaka; Seiji Sakamoto; Sho Wakayama; Yuma Morikawa; Muneo Tsujikawa; Itaru Hamachi

  ACS Chemical Biology, 13 Feb. 2018

  
• Peptide ribonucleic acid (PRNA)Arginine Hybrids. Effects of arginine residues alternatingly introduced to PRNA backbone on aggregation, cellular uptake, and cytotoxicity

  Hiroka Sugai; Ikuhiko Nakase; Seiji Sakamoto; Akihiro Nishio; Masahito Inagaki; Masaki Nishijima; Asako Yamayoshi; Yasuyuki Araki; Satoru Ishibashi; Takanori Yokota; Yoshihisa Inoue; Takehiko Wada

  Chemistry Letters, 2018, Peer-reviewed

  
• Activation of p53 Facilitates the Target Search in DNA by Enhancing the Target Recognition Probability

  Yuji Itoh; Agato Murata; Seiji Sakamoto; Kei Nanatani; Takehiko Wada; Satoshi Takahashi; Kiyoto Kamagata

  Journal of Molecular Biology, Jul. 2016

  
• Module Strategy for Peptide Ribonucleic Acid (PRNA)-DNA and PRNA-Peptide Nucleic Acid (PNA)-DNA Chimeras: Synthesis and Interaction of Chimeras with DNA and RNA

  Ryohei Uematsu; Masahito Inagaki; Mitsuo Asai; Hiroka Sugai; Yoshiki Maeda; Akira Nagami; Hirofumi Sato; Seiji Sakamoto; Yasuyuki Araki; Masaki Nishijima; Yoshihisa Inoue; Takehiko Wada

  CHEMISTRY LETTERS, Mar. 2016, Peer-reviewed

  
• Design and semisynthesis of photoactivable split‐GFP by incorporation of photocleavable functionality

  Seiji Sakamoto; Mika Terauchi; Yasuyuki Araki; Takehiko Wada

  Peptide Science, 26 Nov. 2013

  
• Remarkable Enhancement of Sensitivity with the Second Generation of Elliptically Polarization-detected Circular Dichroism Spectroscopy

  Makoto Murakami; Yasuyuki Araki; Seiji Sakamoto; Yoshiki Hamada; Takehiko Wada

  Chemistry Letters, 23 Feb. 2013

  
• Creation of a caspase-3 sensing system using a combination of split-GFP and split-intein

  Seiji Sakamoto; Mika Terauchi; Anna Hugo; Tanner Kim; Yasuyuki Araki; Takehiko Wada

  Chemical Communications, 2013

  
• Synthesis of Indenes by Intramolecular Morita-Baylis-Hillman Reaction in Aqueous Media Catalyzed by Resin-Supported Proline

  Kazuaki Kudo; Kengo Akagawa; Seiji Sakamoto

  Synlett, 22 Feb. 2011

  
• Novel Strategy of Supramolecular Asymmetric Photochirogenesis with Tailor-Made Biopolymers as Chiral Reaction Fields

  Takehiko Wada; Masaki Nishijima; Seiji Sakamoto; Makoto Murakami; Yui Sugawara; Yasuyuki Araki; Yoshihisa Inoue

  Journal of Photopolymer Science and Technology, 2011

  
• Efficient Asymmetric α-Oxyamination of Aldehydes by Resin-Supported Peptide Catalyst in Aqueous Media

  Kengo Akagawa; Takuma Fujiwara; Seiji Sakamoto; Kazuaki Kudo

  Organic Letters, 17 Mar. 2010

  
• Active Control by External Factors of DNA Recognition Behavior of α-Peptide Ribonucleic Acids Containing Basic Amino Acid Residues

  Takehiko Wada; Nobuya Sawa; Hirofumi Sato; Mayuko Kikkawa; Keiko Onodera; Seiji Sakamoto; Yoshihisa Inoue

  Chemistry Letters, 16 Jan. 2010

  
• One-pot sequential alcohol oxidation and asymmetric α-oxyamination in aqueous media using recyclable resin-supported peptide catalyst

  Kengo Akagawa; Takuma Fujiwara; Seiji Sakamoto; Kazuaki Kudo

  Chemical Communications, 2010

  
• Friedel–Crafts-type alkylation in aqueous media using resin-supported peptide catalyst having polyleucine

  Kengo Akagawa; Takuhiro Yamashita; Seiji Sakamoto; Kazuaki Kudo

  Tetrahedron Letters, Oct. 2009

  
• Asymmetric transfer hydrogenation in aqueous media catalyzed by resin-supported peptide having a polyleucine tether

  Kengo Akagawa; Hajime Akabane; Seiji Sakamoto; Kazuaki Kudo

  Tetrahedron: Asymmetry, Mar. 2009

  
• Derived C2-Symmetric Diketopiperazine as Chiral Ligand for Asymmetric Diels-Alder Reactions

  Masahiro Furutani; Seiji Sakamoto; Kazuaki Kudo

  Heterocycles, 2009

  
• Supramolecular Control of Split-GFP Reassembly by Conjugation of β-Cyclodextrin and Coumarin Units

  Seiji Sakamoto; Kazuaki Kudo

  Journal of the American Chemical Society, 27 Jun. 2008

  
• Glucose Responsive Two-step Release of Hydrogel-immobilized Protein

  Kazutoshi Murota; Seiji Sakamoto; Kazuaki Kudo

  Chemistry Letters, 26 Apr. 2008

  
• Organocatalytic Asymmetric Transfer Hydrogenation in Aqueous Media Using Resin-Supported Peptide Having a Polyleucine Tether

  Kengo Akagawa; Hajime Akabane; Seiji Sakamoto; Kazuaki Kudo

  Organic Letters, 12 Apr. 2008

  
• Reversible Immobilization of Protein into Hydrogel Using Designed Coiled-coil Peptides

  Kazutoshi Murota; Seiji Sakamoto; Kazuaki Kudo

  Chemistry Letters, 06 Oct. 2007

  
• Orientation Control of Self-stacking d,l-Alternating Cyclic Octa-α-peptide through Multiple Electrostatic Interactions

  Kazutoshi Murota; Seiji Sakamoto; Kazuaki Kudo

  Chemistry Letters, 21 Jul. 2007

  
• Resin-supported acid- and base-catalyzed one-pot sequential reaction including an enantioselective step

  Kengo Akagawa; Seiji Sakamoto; Kazuaki Kudo

  Tetrahedron Letters, Feb. 2007

  
• Synthesis of Blue Emitting Alicyclic Polyimides using One-pot Alternating Copolymerization Method

  Kazuaki Kudo; Takahiro Imai; Takashi Hamada; Seiji Sakamoto

  High Performance Polymers, Oct. 2006

  
• One‐Pot Synthesis of an Alternating Copolyimide Based on Regioselective Reaction of a Non‐Symmetrical Alicyclic Dianhydride

  Kazuaki Kudo; Takuya Yoshizawa; Takashi Hamada; Jun Li; Seiji Sakamoto; Shinsaku Shiraishi

  Macromolecular Rapid Communications, 31 Aug. 2006

  
• Regulation of Catalytic Activity of Peptide–Heme Conjugate by Conformational Change with Trifluoroethanol

  Seiji Sakamoto; Hidekazu Fukushima; Kazuaki Kudo

  Chemistry Letters, 29 Apr. 2006

  
• Direct asymmetric aldol reaction in aqueous media using polymer-supported peptide

  Kengo Akagawa; Seiji Sakamoto; Kazuaki Kudo

  Tetrahedron Letters, Nov. 2005

  
• Design and Synthesis of Semi-Artificial Myoglobin Possessing DNA-Binding Peptides on Heme Propionates

  Seiji Sakamoto; Kazuaki Kudo

  Bulletin of the Chemical Society of Japan, 01 Oct. 2005

  
• Artificial Assembly of Myoglobin and Flavodoxin Reductase Using Designed Coiled-coil Peptides

  Seiji Sakamoto; Atsushi Itoh; Kazuaki Kudo

  Chemistry Letters, 21 Aug. 2004

  
• De Novo Design, Synthesis, and Function of Semiartificial Myoglobin Conjugated with Coiled‐Coil Two‐α‐Helix Peptides

  Seiji Sakamoto; Atsushi Ito; Kazuaki Kudo; Susumu Yoshikawa

  Chemistry – A European Journal, 22 Jul. 2004

  
• Design of FAD-binding Peptide Using a Combinatorial α-Helix Peptide Library

  Seiji Sakamoto; Ippei Okano; Kazuaki Kudo

  Chemistry Letters, 05 Jul. 2004

  
• Design, synthesis and peroxidase-like activity of 3α-helix proteins covalently bound to heme

  Ikuo Obataya; Tomoyuki Kotaki; Seiji Sakamoto; Akihiko Ueno; Hisakazu Mihara

  Bioorganic & Medicinal Chemistry Letters, Dec. 2000

  
• Construction ofα-Helix Peptides withβ-Cyclodextrin and Dansyl Units and Their Conformational and Molecular Sensing Properties

  Sachiko Matsumura; Seiji Sakamoto; Akihiko Ueno; Hisakazu Mihara

  Chemistry - A European Journal, 15 May 2000

  
• Electron Transfer of Flavin-pendant α-Helical Peptides Self-assembled on an Electrode

  Tetsuya HARUYAMA; Seiji SAKAMOTO; Hisakazu MIHARA; Masuo AIZAWA

  Electrochemistry, 05 Dec. 1999

  
• Nanoparticle Formation of Self-Assembled Two-α-Helix Peptide Induced by Heme-Bindin

  Ikuo Obataya; Seiji Sakamoto; Akihiko Ueno; Hisakazu Mihara

  Protein and Peptide Letters, 1999, Peer-reviewed

  
• Regulation of α/β-folding of a designed peptide by haem binding

  Seiji Sakamoto; Ikuo Obataya; Akihiko Ueno; Hisakazu Mihara

  Chemical Communications, 1999

  
• Effects of amino acids substitution of hydrophobic residues on haem-binding properties of designed two-α-helix peptides

  Seiji Sakamoto; Ikuo Obataya; Akihiko Ueno; Hisakazu Mihara

  Journal of the Chemical Society, Perkin Transactions 2, 1999

  
• Annealing of Two-α-Helix Structure by Metal Ion Binding Regulated with Trifluoroethanol

  Seiji Sakamoto; Akihiko Ueno; Hisakazu Mihara

  Chemistry Letters, 01 Sep. 1998

  
• Molecular assembly of two-α-helix peptide induced by haem binding

  Seiji Sakamoto; Akihiko Ueno; Hisakazu Mihara

  Chemical Communications, 1998

  
• Design and synthesis of haem-binding peptides. Relationship between haem-binding properties and catalytic activities

  Seiji Sakamoto; Akihiko Ueno; Hisakazu Mihara

  Journal of the Chemical Society, Perkin Transactions 2, 1998

  
• Haem binding and catalytic activity of two-α-helix peptide annealed by trifluoroethanol

  Seiji Sakamoto; Shigeo Sakurai; Akihiko Ueno; Hisakazu Mihara

  Chemical Communications, 1997

  
• Artificial Membrane Protein Functionalized with Electron Transfer System

  Hisakazu Mihara; Kin-ya Tomizaki; Tsutomu Fujimoto; Seiji Sakamoto; Haruhiko Aoyagi; Norikazu Nishino

  Chemistry Letters, 01 Mar. 1996

  
• Chiral Assembly of Porphyrins Regulated by Amphiphilic α-Helix Peptides

  Hisakazu Mihara; Yasuaki Haruta; Seiji Sakamoto; Norikazu Nishino; Haruhiko Aoyagi

  Chemistry Letters, 01 Jan. 1996

  
• Design and synthesis of flavin-conjugated peptides and assembly on a gold electrode

  Seiji Sakamoto; Haruhiko Aoyagi; Naotoshi Nakashima; Hisakazu Mihara

  Journal of the Chemical Society, Perkin Transactions 2, 1996

  
• Enhanced Membrane-Perturbing Activities of Bundled Amphiphilic α-Helix Polypeptides on Interaction with Phospholipid Bilayer

  Seiji Sakamoto; Hisakazu Mihara; Eitarou Matsuo; Takuro Niidome; Kazunori Anzai; Yutaka Kirino; Haruhiko Aoyagi

  Bulletin of the Chemical Society of Japan, 01 Oct. 1995

  
• Peptide Synthesis Mediated by Thiolsubtilisin Using Peptide Thioester as Building Block

  Hisakazu Mihara; Shuko Maeda; Ryo Kurosaki; Satoko Ueno; Seiji Sakamoto; Takuro Niidome; Hironobu Hojo; Saburo Aimoto; Haruhiko Aoyagi

  Chemistry Letters, 01 May 1995

  

Misc.

• Protocol to visualize the distribution of exogenously administered small molecules in the mouse brain

  Takeharu Mino; Hiroshi Nonaka; Seiji Sakamoto; Jae Hoon Oh; Itaru Hamachi

  STAR Protocols, Sep. 2023

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• Ligand‐Directed Chemistry for Protein Labeling for Affinity‐Based Protein Analysis

  Seiji Sakamoto; Itaru Hamachi

  Israel Journal of Chemistry, 06 Feb. 2023

  
• Recent applications of N-acyl imidazole chemistry in chemical biology

  Takeharu Mino; Seiji Sakamoto; Itaru Hamachi

  Bioscience, Biotechnology, and Biochemistry, 07 Jan. 2021

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• Construction of ligand assay systems by protein-based semisynthetic biosensors

  Seiji Sakamoto; Shigeki Kiyonaka; Itaru Hamachi

  Current Opinion in Chemical Biology, Jun. 2019

  
• Recent Progress in Chemical Modification of Proteins

  Seiji Sakamoto; Itaru Hamachi

  Analytical Sciences, 12 Oct. 2018

  
• De Novo Design and Synthesis of Myoglobin-Peptide Conjugate

  Peptide Science 2002, 2003

  
• Biochemical Characterization of Flavodoxin-Like Protein from Hyper-Thermophilic Bacterium Aquifx aeolicus

  Peptide Sciene 2001, 2002

  
• Design and Synthesis of 3a-Helix Bundle Peptides Possessing a Hydrophobic

  Peptide Science 1999, 2000

  
• Construction of cyclodextrin-peptide conjugates and their molecular sensing properties

  S Matsumura; S Sakamoto; A Ueno; H Mihara

  PEPTIDE SCIENCE - PRESENT AND FUTURE, 1999

  
• Self-assembly of heme-conjugated two-alpha-helix peptides

  Obtaya, I; S Sakamoto; A Ueno; H Mihara

  PEPTIDE SCIENCE - PRESENT AND FUTURE, 1999

  
• Design, synthesis and characterization of heme-conjugated two-alpha-helix peptides

  S Sakamoto; A Ueno; H Mihara

  PEPTIDE SCIENCE - PRESENT AND FUTURE, 1999

  
• De Novo Design, Synthesis and Characterization of Heme-Conjugated Two-a-Helix Peptides

  Peptide Science 1998, 1999

  
• Supramolecular Assembly of a-Helix Peptide Conjugated with Porphyrins

  Peptides 1996, 1998

  
• Construction of Heme-Binding Two-a-Helix Peptides and Regulation of Binding Property by Peptide Conformation

  J. Inorg. Chem., 1997

  
• Design and Synthesis of Haem-Binding Molecular Forceps Composed of Two-a-Helix Peptide

  Peptide Chemistry 1996, 1997

  

Presentations

• Rapid labeling strategy for endogenous glutamate receptors by a combination of in-brain ligand-directed acyl imidazole chemistry and IEDDA click reaction

  Seiji Sakamoto; Kazuki Shiraiwa; Hiroshi Nonaka; Itaru Hamachi

  ISBC202, 24 Apr. 2024

  
• 脳内リガンド指向性化学とクリックケミストリーの組み合わせによるグルタミン酸受容体の高速ラベル化戦略

  坂本清志; 白岩和樹; 野中洋; 浜地格

  日本化学会 第104春季年会, 20 Mar. 2024

  
• Ligand directed chemistry in live mouse brain (2): 様々な神経伝達物質受容体への拡張と寿命解析

  坂本 清志; 白岩 和樹; 清中 茂樹; 野中 洋; 浜地 格

  日本化学会 第102春季年会, 24 Mar. 2022

  
• 内存性GABA(A) 受容体のマウス脳内固定化駆動ケミカルラベリング

  坂本清志; 原田文雄; 清中茂樹; 野中洋; 浜地格

  日本化学会 第101春季年会, 19 Mar. 2021

  
• リガンド指向性化学による内存性GABA(A)受容体の選択的ケミカルラベリング

  坂本清志; 原田文峰; 清中茂樹; 野中洋; 浜地格

  22 Mar. 2020

  
• A Turn-on Imaging Probe for GABA(A) Receptors Allows for Construction of Fluorescent Biosensor

  Seiji SAKAMOTO; Fumio HARADA; Kei YAMAURA; Kazuma AMAIKE; Tomohiro NUMATA; Ryuji INOUE; Shigeki KIYONAKA; Itaru HAMACHI

  日本化学会第99春季年会, 16 Mar. 2019

  
• Construction of fluorescent biosensor using a turn-on-type imaging probe for GABA(A) receptors to discover allosteric modulators

  Seiji Sakamoto; Fumio Harada; Kazuma Amaike; Kei Yamaura; Shigeki Kiyonaka; Itaru Hamachi

  10th International Peptide Symposium, 03 Dec. 2018

  
• Ligand Directed Acyl Imidazole Chemistry for Drug Sensing of AMPA-Type Glutamate Receptor

  Seiji Sakamoto; Sho Wakayama; Shigeki Kiyonaka; Itaru Hamachi

  日本化学会第98春季年会, 20 Mar. 2018

  
• Ligand Directed Acyl Imidazole Chemistry for Drug Assay of AMPA-Type Glutamate Receptor

  Seiji Sakamoto; Sho Wakayama; Shigeki Kiyonaka; Itaru Hamachi

  ISBC2017, 14 Dec. 2017

  
• リガンド指向性化学のAMPA型グルタミン酸受容体薬剤アッセイへの展開

  坂本清志; 若山翔; 清中茂樹; 浜地格

  第11回バイオ関連化学シンポジウム, 07 Sep. 2017

  

External funds: Kakenhi

• Construction of ligand assay system for GABA(A) receptors using ligand-directed chemistry

  Grant-in-Aid for Scientific Research (C)

  Basic Section 37030:Chemical biology-related

  Kyoto University

  Seiji Sakamoto

  From 01 Apr. 2020, To 31 Mar. 2023, Project Closed

  GABA(A)受容体;リガンド指向性化学;タンパク質ラベル化;バイオセンサー;イメージングプローブ;蛍光イメージング;内在性受容体;分子夾雑化学;スクリーニング;脳化学;神経化学;ケミカルラベル;イメージング;タンパク質化学修飾

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K05746/
• Development of New Strategy of Protein Design Based on Single Phage Sorting Technique

  Grant-in-Aid for Scientific Research (B)

  Tohoku University

  Satoshi TAKAHASHI

  From 01 Apr. 2014, To 31 Mar. 2017, Project Closed

  タンパク質デザイン;ファージ;一分子蛍光観察;ファージディスプレイ;一分子ソーター;GFP;タンパク質;生物・生体工学;生物物理;一分子計測;蛍光タンパク質;一分子選別装置;ファージディスプレイ実験;分割型GFP

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26282213/
• Design of Detection System for Histone Modification Patterns Using Split-Proteins

  Grant-in-Aid for Challenging Exploratory Research

  Tohoku University

  Seiji SAKAMOTO

  From 01 Apr. 2013, To 31 Mar. 2015, Project Closed

  エピジェネティクス;ヒストンテール;翻訳後修飾;分割型タンパク質;GFP;ルシフェラーゼ;バイオプローブ;ヒストン;分割型緑色蛍光タンパク質

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25560412/
• Supramolecular Enantiodifferentiating Photocyclodimerization of 2-Anthracenecarboxylate with Proteins and Protein-Polymer Hybrids as Chiral Reaction Medias

  Grant-in-Aid for Scientific Research (A)

  Tohoku University

  Takehiko WADA

  From 01 Apr. 2012, To 31 Mar. 2015, Project Closed

  生体高分子;血清アルブミン;キラル反応場;不斉合成;光反応;超分子;超分子不斉光反応;PEG;合成坑体;不斉光反応;超分子不斉光化学;人工坑体;2-アントラセンカルボン酸;ファージディスプレイ法;キラルソフト界面

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24245044/
• Creation of A Multi-Color Sensing System for Protease Activities.

  Grant-in-Aid for Challenging Exploratory Research

  Tohoku University

  Seiji SAKAMOTO

  From 01 Apr. 2011, To 31 Mar. 2013, Project Closed

  プロテアーゼ;蛍光タンパク質;発光タンパク質;バイオプローブ;バイオセンサー;分割型タンパク質;分割型蛍光タンパク質;分割型インテイン;カスパーゼ;アポトーシス

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23651230/
• Design and synthesis of histone tail polypeptide library

  Grant-in-Aid for Young Scientists (B)

  Tohoku University;The University of Tokyo

  Seiji SAKAMOTO

  From 01 Apr. 2007, To 31 Mar. 2009, Project Closed

  生体機能関連物質;ポリペプチド;ライブラリ;エピジェネティクス;ヒストンテール;ライブリ;分割型GFP

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19710181/
• タンパク質活性中心近傍における特異的基質認識部位・機能制御部位の人工構築

  Grant-in-Aid for Young Scientists (B)

  The University of Tokyo

  坂本 清志

  From 01 Apr. 2005, To 31 Mar. 2007, Project Closed

  タンパク質;ポリペプチド;ライブラリ;人工酵素;補因子;ヘムタンパク質;コンビナトリアル化学;電子移動

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17710180/
• 特異的糖鎖構造認識能を有する機能性ペプチドアレイの構築

  Grant-in-Aid for Young Scientists (B)

  The University of Tokyo

  坂本 清志

  From 01 Apr. 2003, To 31 Mar. 2005, Project Closed

  α-ヘリックス;β-ターン;ポリペプチド;ライブラリ;機能性アレイ;ボロン酸;糖認識;デノボ設計

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15710157/
• 内在性GABA(A)受容体のケミカルラベルを基軸とする薬剤アッセイ系の構築

  Grant-in-Aid for Scientific Research (C)

  Basic Section 37030:Chemical biology-related

  Kyoto University

  坂本 清志

  From 01 Apr. 2023, To 31 Mar. 2026, Granted

  GABA(A)受容体;リガンド指向性化学;タンパク質ラベル化;バイオセンサー;蛍光イメージング;神経伝達物質受容体;ケミカルラベリング

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K04960/