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Hara, Ryotaro
Graduate School of Agriculture Program-Specific Associate Professor
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Last Updated :2025/06/20
Basic Information
Faculty
Academic Degree
Research History
- Waseda University Faculty of Science and Engineering Waseda Research Institute for Science and Engineering, Junior Researcher(Assistant Professor)
Language of Instruction
ID,URL
ORCID ID
J-Global ID
Researcher number
70553535
researchmap URL
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Last Updated :2025/06/20
Research
Research Areas
Papers
- One-Pot Synthesis of Useful S-Substituted-
l -cysteine Sulfoxides Using Genetically Engineered Escherichia coliTaku Mizutani; Ryotaro Hara; Michiki Takeuchi; Makoto Hibi; Makoto Ueda; Jun OgawaJournal of Agricultural and Food Chemistry, 28 Feb. 2024
- Screening, gene cloning, and characterization of orsellinic acid decarboxylase from Arthrobacter sp. K8 for regio-selective carboxylation of resorcinol derivativesKuniki Kino; Yasutaka Hirokawa; Ryo Gawasawa; Ryota Murase; Ryohei Tsuchihashi; Ryotaro HaraJournal of Biotechnology, 10 Nov. 2020
- 腸内細菌における食事成分・薬効成分代謝の解析と代謝物の生理機能小川 順; 原 良太郎; 安藤 晃規; 竹内 道樹; 岸野 重信生化学, 25 Aug. 2023, Peer-reviewed
- Identification of tryptophanase from Escherichia coli for the synthesis of S-allyl-l-cysteine and related S-substituted cysteine derivativesTaku Mizutani; Ryotaro Hara; Takayuki Iihoshi; Shoko Kozono; Michiki Takeuchi; Makoto Hibi; Satomi Takahashi; Makoto Ueda; Jun OgawaJournal of Bioscience and Bioengineering, Jun. 2022
- Enzymatic Synthesis of l-threo-β-Hydroxy-α-Amino Acids via Asymmetric Hydroxylation Using 2-Oxoglutarate-Dependent Hydroxylase from Sulfobacillus thermotolerans Y0017.Ryotaro Hara; Yuta Nakajima; Hiroaki Yanagawa; Ryo Gawasawa; Izumi Hirasawa; Kuniki KinoApplied and environmental microbiology, 04 Aug. 2021
- A three-component monooxygenase from Rhodococcus wratislaviensis may expand industrial applications of bacterial enzymes.Makoto Hibi; Dai Fukuda; Chihiro Kenchu; Masutoshi Nojiri; Ryotaro Hara; Michiki Takeuchi; Shunsuke Aburaya; Wataru Aoki; Kimihiko Mizutani; Yoshihiko Yasohara; Mitsuyoshi Ueda; Bunzo Mikami; Satomi Takahashi; Jun OgawaCommunications biology, 04 Jan. 2021
- Enzymatic reactions and microorganisms producing the various isomers of hydroxyprolineRyotaro Hara; Kuniki KinoApplied Microbiology and Biotechnology, 15 Apr. 2020, Peer-reviewed
- Ectoine hydroxylase displays selective trans-3-hydroxylation activity towards l-prolineRyotaro Hara; Takeyuki Nishikawa; Takuya Okuhara; Kento Koketsu; Kuniki KinoApplied Microbiology and Biotechnology, 20 Jul. 2019, Peer-reviewed
- Synthesis of D-Amino Acid-Containing Dipeptides Using the Adenylation Domains of Nonribosomal Peptide SynthetaseSoichiro Kano; Shin Suzuki; Ryotaro Hara; Kuniki Kino; Claire VieilleApplied and Environmental Microbiology, 19 Apr. 2019, Peer-reviewed
- A chemoenzymatic process for amide bond formation by an adenylating enzyme-mediated mechanismRyotaro Hara; Kengo Hirai; Shin Suzuki; Kuniki KinoScientific Reports, 01 Dec. 2018, Peer-reviewed
- Production of aminoacyl prolines using the adenylation domain of nonribosomal peptide synthetase with class III polyphosphate kinase 2-mediated ATP regenerationShin Suzuki; Ryotaro Hara; Kuniki KinoJournal of Bioscience and Bioengineering, 01 Jun. 2018, Peer-reviewed
- Discovery of lysine hydroxylases in the clavaminic acid synthase-like superfamily for efficient hydroxylysine bioproductionRyotaro Hara; Kai Yamagata; Ryoma Miyake; Hiroshi Kawabata; Hisatoshi Uehara; Kuniki KinoApplied and Environmental Microbiology, 01 Sep. 2017, Peer-reviewed
- Functional characterization of aconitase X as a cis-3-hydroxy-L-proline dehydrataseSeiya Watanabe; Kunihiko Tajima; Satoshi Fujii; Fumiyasu Fukumori; Ryotaro Hara; Rio Fukuda; Mao Miyazaki; Kuniki Kino; Yasuo WatanabeScientific Reports, 08 Dec. 2016, Peer-reviewed
- Development of a multi-enzymatic cascade reaction for the synthesis of trans-3-hydroxy-l-proline from l-arginineRyotaro Hara; Saki Kitatsuji; Kai Yamagata; Kuniki KinoApplied Microbiology and Biotechnology, 01 Jan. 2016, Peer-reviewed
- One-Pot Production of l-threo-3-Hydroxyaspartic Acid Using Asparaginase-Deficient Escherichia coli Expressing Asparagine Hydroxylase of Streptomyces coelicolor A3(2)Ryotaro HaraApplied and Environmental Microbiology, 01 Jun. 2015, Peer-reviewed
- Hydroxamate-based colorimetric assay to assess amide bond formation by adenylation domain of nonribosomal peptide synthetasesRyotaro HaraAnalytical Biochemistry, May 2015, Peer-reviewed
- Refined regio- and stereoselective hydroxylation of l -pipecolic acid by protein engineering of l -proline cis -4-hydroxylase based on the X-ray crystal structureKento Koketsu; Yasuhito Shomura; Kei Moriwaki; Mikiro Hayashi; Satoshi Mitsuhashi; Ryotaro Hara; Kuniki Kino; Yoshiki HiguchiACS Synthetic Biology, 17 Apr. 2015, Peer-reviewed
- Identification and characterization of 2-oxoglutarate-dependent dioxygenases catalyzing selective cis-hydroxylation of proline and pipecolinic acid from actinomycetesRyotaro HaraJournal of Biotechnology, Feb. 2014, Peer-reviewed
- Regio- and stereoselective oxygenation of proline derivatives by using microbial 2-oxoglutarate-dependent dioxygenasesRyotaro Hara; Naoko Uchiumi; Naoko Okamoto; Kuniki KinoBioscience, Biotechnology and Biochemistry, 2014, Peer-reviewed
- Microbial production of N-acetyl cis-4-hydroxy-l-proline by coexpression of the Rhizobium l-proline cis-4-hydroxylase and the yeast N-acetyltransferase Mpr1Thi Mai Hoa Bach; Ryotaro Hara; Kuniki Kino; Iwao Ohtsu; Nobuyuki Yoshida; Hiroshi TakagiApplied Microbiology and Biotechnology, Jan. 2013, Peer-reviewed
- Enhanced synthesis of 5-hydroxy-l-tryptophan through tetrahydropterin regenerationRyotaro HaraAMB Express, 2013, Peer-reviewed
- Industrial Production of threo-3-Hydroxy-L-Aspartic Acid Using Escherichia coli Resting CellsRyotaro HaraJournal of Biotechnology, Nov. 2010, Peer-reviewed
- Enhancement of l-tryptophan 5-hydroxylation activity by structure-based modification of l-phenylalanine 4-hydroxylase from Chromobacterium violaceumKuniki Kino; Ryotaro Hara; Ai NozawaJournal of Bioscience and Bioengineering, Sep. 2009, Peer-reviewed
- Characterization of novel 2-oxoglutarate dependent dioxygenases converting l-proline to cis-4-hydroxy-l-prolineRyotaro Hara; Kuniki KinoBiochemical and Biophysical Research Communications, 20 Feb. 2009, Peer-reviewed
- Two groups of thermophilic amino acid aminotransferases exhibiting broad substrate specificities for the synthesis of phenylglycine derivativesDaisuke Koma; Toshiya Sawai; Ryotaro Hara; Shigeaki Harayama; Kuniki KinoApplied Microbiology and Biotechnology, Jul. 2008, Peer-reviewed
- Two groups of thermophilic amino acid aminotransferases exhibiting broad substrate specificities for the synthesis of phenylglycine derivativesRyotaro HaraApplied Microbiology and Biotechnology, Jul. 2008, Peer-reviewed
- A high-throughput and generic assay method for the determination of substrate specificities of thermophilic α-aminotransferasesToshiya Sawai; Daisuke Koma; Ryotaro Hara; Kuniki Kino; Shigeaki HarayamaJournal of Microbiological Methods, Oct. 2007, Peer-reviewed
Misc.
- 1Ep08 One-pot synthesis of L-threo-3-hydroxyaspartic acid using Escherichia coli overexpressing amino acid hydroxylase gene from Streptomyces coelicolor A3(2)Hara Ryotaro; Kino Kuniki日本生物工学会大会講演要旨集, 2011
- 2P-1067 Synthesis of L-threo-3-hydroxyaspartic acid with a recombinant Escherichia coli whole-cell reactionHARA Ryotaro; KINO Kuniki日本生物工学会大会講演要旨集, 2010
- 1P-058 Synthesis of various hydroxyamino acids by microbial 2-oxoglutaratedependent dioxygenasesHara Ryotaro; Nakajima Yuta; Kitatsuji Saki; Yamagata Kai; Gawasawa Ryo; Kino Kuniki日本生物工学会大会講演要旨集, 2015
- 1Dp12 Production of czs-4-hydroxy-L-proline using Escherichia coli expressing a novel L-proline cis-4-hydroxylase geneOKAMOTO Naoko; HARA Ryotaro; KINO Kuniki日本生物工学会大会講演要旨集, 2009
- 微生物ダークマターとマイクロバイオータ研究が導く新しい生物工学 物質循環・代謝におけるマイクロバイオータ機能の具現化ツール開発小川 順; 岸野 重信; 安藤 晃規; 竹内 道樹; 原 良太郎; 上田 誠日本生物工学会大会講演要旨集, Aug. 2019
- 1P-104 Discovery of novel microbial lysine hydroxylasesHara Ryotaro; Yamagata Kai; Miyake Ryoma; Kawabata Hiroshi; Kino Kuniki日本生物工学会大会講演要旨集, 2014
- 1P-105 Characterization of ornithine cyclodeaminase and enzymatic synthesis of valuable hydroxyimino acidsKitatsuji Saki; Hara Ryotaro; Kino Kuniki日本生物工学会大会講演要旨集, 2014
- 1P-079 Characterization of Microbial Hydroxylases for Efficient Synthesis of Valuable Hydroxy Imino AcidsHara Ryotaro; Uchiumi Naoko; Kino Kuniki日本生物工学会大会講演要旨集, 2013
- 2Cp19 Characterization of Rhizobial Hydroxyproline Epimerase and Synthesis of D-HydroxyprolineHara Ryotaro; Kino Kuniki日本生物工学会大会講演要旨集, 2012
- 3Cp06 Synthesis of amino acid amides by a module of non-ribosomal peptide synthetaseSato Masaru; Hara Ryotaro; Kino Kuniki日本生物工学会大会講演要旨集, 2012
- 4Dp10 Microbial production of N-acetyl cis-4-hydroxy-L-proline by coexpression of the Rhizobium L-proline cis-4-hydroxylase and the yeast N-acetyltranferase Mpr1 :Hoa Bach Thi Mai; Hara Ryotaro; Kino Kuniki; Takagi Hiroshi日本生物工学会大会講演要旨集, 2012
External funds: Kakenhi
- Exploring novel glucosidase for glucoside synthesis and the characterization.Grant-in-Aid for Scientific Research (C)Basic Section 38020:Applied microbiology-relatedOyama National College of TechnologyMakoto UEDAFrom 01 Apr. 2021, To 31 Mar. 2024, Project Closed配糖体;β-グルコシド結合;β-グルコシダーゼ;糖転移反応;微生物;グルコシダーゼ;アノマー;微生物変換;転移反応
- Screening of microbial amide-bond forming enzyme for stabilization and functionalization of bioactive fatty acidsGrant-in-Aid for Scientific Research (C)Basic Section 38020:Applied microbiology-relatedKyoto UniversityRyotaro HaraFrom 01 Apr. 2021, To 31 Mar. 2024, Project Closed脂肪酸アミド;アミド結合;セリンペプチダーゼ;オレアミド;エルカミド;Mycobacterium;スクリーニング;アミダーゼ;アミド化合物;脂質メディエーター;酵素法;酵素精製;serine protease;酵素;探索;物質生産;高度不飽和脂肪酸;アミド化;微生物酵素;バイオ生産
- Production of (+)-alliin by tandem enzymatic reactionsGrant-in-Aid for Scientific Research (C)Basic Section 38020:Applied microbiology-relatedToyama Prefectural UniversityMakoto HibiFrom 01 Apr. 2019, To 31 Mar. 2022, Project Closedファイトケミカル;バイオプロセス;S-アリルシステインスルフォキシド;(+)-アリイン
- Development of screening tool for amino acid hydroxylase using insufficient TCA-cycle complementation test and its applicationGrant-in-Aid for Scientific Research (C)Basic Section 38020:Applied microbiology-relatedKyoto UniversityRyotaro HaraFrom 01 Apr. 2018, To 31 Mar. 2021, Project ClosedScreening;Hydroxylase;Enzyme;Hydroxy amino acid;Dioxygenase;3-Hydroxyhistidine;3-Hydroxyglutamine;Insufficient TCA-cycle;3-Hydroxyhisitidine;Broken TCA cycle;dioxygenase;screening;amino acid;2-oxoglutarate;hydroxylation;trans-3-hydroxyproline;3-hydroxyhistidine;3-hydroxyglutamine;hydroxy amino acid;Escherichia coli;bioproduction;hydroxylase
- Enzymatic synthesis of useful hydroxyproline derivatives by regio- and stereo-selective hydroxylationGrant-in-Aid for Young Scientists (B)Waseda UniversityRyotaro HARAFrom 01 Apr. 2015, To 31 Mar. 2017, Project Closedプロリン;ヒドロキシプロリン;エクトイン;水酸化酵素;微生物変換;trans-3-ヒドロキシ-L-プロリン;ヒドロキシエクトイン;アミノ酸水酸化酵素;エクトイン水酸化酵素;2-オキソグルタル酸依存型ジオキシゲナーゼ
- Development of production process for sulfer containing amino acids using microbial enzymesGrant-in-Aid for Scientific Research (C)Basic Section 38020:Applied microbiology-relatedKyoto University原 良太郎From 01 Apr. 2024, To 31 Mar. 2027, Granted含硫アミノ酸;S-置換システイン誘導体;合成酵素;酸化酵素;ラセミ化酵素
- Analysis of aldehyde chemistry in gutGrant-in-Aid for Scientific Research (A)Medium-sized Section 38:Agricultural chemistry and related fieldsKyoto University小川 順From 01 Apr. 2024, To 31 Mar. 2027, Grantedaldehyde;oxidoreduction;dehydroxylase;fatty acid;plasmalogen
list
Last Updated :2025/06/20
Education
Teaching subject(s)
- From 01 Apr. 2025, To 31 Mar. 2026Industrial Microbiology (Lecture)CA05, Spring, Graduate School of Agriculture, 1
- From 01 Apr. 2025, To 31 Mar. 2026Fermentation Physiology and Applied Microbiology SeminarCF06, Fall, Graduate School of Agriculture, 1.5
- From 01 Apr. 2025, To 31 Mar. 2026Fermentation Physiology and Applied Microbiology SeminarCF06, Spring, Graduate School of Agriculture, 1.5
- From 01 Apr. 2025, To 31 Mar. 2026Experimental Course of Fermentation Physiology and Applied MicrobiologyCG06, Spring, Graduate School of Agriculture, 3
- From 01 Apr. 2025, To 31 Mar. 2026Experimental Course of Fermentation Physiology and Applied MicrobiologyCG06, Fall, Graduate School of Agriculture, 3
- From 01 Apr. 2025, To 31 Mar. 2026Laboratory Course in Biochemistry5669, Spring, Faculty of Agriculture, 3
- From 01 Apr. 2025, To 31 Mar. 2026Industrial Microbiology5666, Fall, Faculty of Agriculture, 2
- From 01 Apr. 2024, To 31 Mar. 2025Industrial Microbiology (Lecture)CA05, Spring, Graduate School of Agriculture, 1
- From 01 Apr. 2024, To 31 Mar. 2025Fermentation Physiology and Applied Microbiology SeminarCF06, Spring, Graduate School of Agriculture, 1.5
- From 01 Apr. 2024, To 31 Mar. 2025Fermentation Physiology and Applied Microbiology SeminarCF06, Fall, Graduate School of Agriculture, 1.5
- From 01 Apr. 2024, To 31 Mar. 2025Experimental Course of Fermentation Physiology and Applied MicrobiologyCG06, Spring, Graduate School of Agriculture, 3
- From 01 Apr. 2024, To 31 Mar. 2025Experimental Course of Fermentation Physiology and Applied MicrobiologyCG06, Fall, Graduate School of Agriculture, 3
- From 01 Apr. 2024, To 31 Mar. 2025Laboratory Course in Biochemistry5669, Spring, Faculty of Agriculture, 3
- From 01 Apr. 2024, To 31 Mar. 2025Industrial Microbiology5666, Fall, Faculty of Agriculture, 2
- From 01 Apr. 2023, To 31 Mar. 2024Industrial Microbiology (Lecture)CA05, Spring, Graduate School of Agriculture, 1
- From 01 Apr. 2023, To 31 Mar. 2024Laboratory Course in Biochemistry5669, Year-long, Faculty of Agriculture, 3
- From 01 Apr. 2023, To 31 Mar. 2024Fermentation Physiology and Applied Microbiology Seminar2CB30, Year-long, Graduate School of Agriculture, 3
- From 01 Apr. 2023, To 31 Mar. 2024Fermentation Physiology and Applied Microbiology Seminar1CB29, Year-long, Graduate School of Agriculture, 3
- From 01 Apr. 2023, To 31 Mar. 2024Experimental Course of Fermentation Physiology and Applied Microbiology2CC30, Year-long, Graduate School of Agriculture, 6
- From 01 Apr. 2023, To 31 Mar. 2024Experimental Course of Fermentation Physiology and Applied Microbiology1CC29, Year-long, Graduate School of Agriculture, 6
- From 01 Apr. 2023, To 31 Mar. 2024Industrial Microbiology5666, Fall, Faculty of Agriculture, 2
- From 01 Apr. 2022, To 31 Mar. 2023Fermentation Physiology and Applied Microbiology Seminar2CB30, Year-long, Graduate School of Agriculture, 3
- From 01 Apr. 2022, To 31 Mar. 2023Experimental Course of Fermentation Physiology and Applied Microbiology2CC30, Year-long, Graduate School of Agriculture, 6
- From 01 Apr. 2022, To 31 Mar. 2023Fermentation Physiology and Applied Microbiology Seminar1CB29, Year-long, Graduate School of Agriculture, 3
- From 01 Apr. 2022, To 31 Mar. 2023Industrial Microbiology5666, Fall, Faculty of Agriculture, 2
- From 01 Apr. 2022, To 31 Mar. 2023Laboratory Course in Biochemistry5669, Year-long, Faculty of Agriculture, 3
- From 01 Apr. 2022, To 31 Mar. 2023Industrial Microbiology (Lecture)CA05, Spring, Graduate School of Agriculture, 1
- From 01 Apr. 2022, To 31 Mar. 2023Experimental Course of Fermentation Physiology and Applied Microbiology1CC29, Year-long, Graduate School of Agriculture, 6
- From Apr. 2018, To Mar. 2019Laboratory Course in Applied MicrobiologyFall, 農学部
- From Apr. 2018, To Mar. 2019Industrial MicrobiologyFall, 農学部
- From Apr. 2018, To Mar. 2019Industrial Microbiology (Lecture)Spring, 農学研究科
- From Apr. 2018, To Mar. 2019Experimental Course of Fermentation Physiology and Applied Microbiology1Year-long, 農学研究科
- From Apr. 2018, To Mar. 2019Experimental Course of Fermentation Physiology and Applied Microbiology2Year-long, 農学研究科
- From Apr. 2018, To Mar. 2019Fermentation Physiology and Applied Microbiology Seminar1Year-long, 農学研究科
- From Apr. 2018, To Mar. 2019Fermentation Physiology and Applied Microbiology Seminar2Year-long, 農学研究科
- From Apr. 2019, To Mar. 2020Applied Microbiology IISpring, 農学部
- From Apr. 2019, To Mar. 2020Laboratory Course in Applied MicrobiologyFall, 農学部
- From Apr. 2019, To Mar. 2020Industrial MicrobiologyFall, 農学部
- From Apr. 2019, To Mar. 2020Industrial Microbiology (Lecture)Spring, 農学研究科
- From Apr. 2019, To Mar. 2020Experimental Course of Fermentation Physiology and Applied Microbiology1Year-long, 農学研究科
- From Apr. 2019, To Mar. 2020Experimental Course of Fermentation Physiology and Applied Microbiology2Year-long, 農学研究科
- From Apr. 2019, To Mar. 2020Fermentation Physiology and Applied Microbiology Seminar1Year-long, 農学研究科
- From Apr. 2019, To Mar. 2020Fermentation Physiology and Applied Microbiology Seminar2Year-long, 農学研究科
- From Apr. 2020, To Mar. 2021Applied Microbiology IISpring, 農学部
- From Apr. 2020, To Mar. 2021Laboratory Course in Applied MicrobiologyYear-long, 農学部
- From Apr. 2020, To Mar. 2021Industrial MicrobiologyFall, 農学部
- From Apr. 2020, To Mar. 2021Industrial Microbiology (Lecture)Spring, 農学研究科
- From Apr. 2020, To Mar. 2021Experimental Course of Fermentation Physiology and Applied Microbiology1Year-long, 農学研究科
- From Apr. 2020, To Mar. 2021Experimental Course of Fermentation Physiology and Applied Microbiology2Year-long, 農学研究科
- From Apr. 2020, To Mar. 2021Fermentation Physiology and Applied Microbiology Seminar1Year-long, 農学研究科
- From Apr. 2020, To Mar. 2021Fermentation Physiology and Applied Microbiology Seminar2Year-long, 農学研究科
- From Apr. 2021, To Mar. 2022Laboratory Course in BiochemistryYear-long, 農学部
- From Apr. 2021, To Mar. 2022Industrial MicrobiologyFall, 農学部
- From Apr. 2021, To Mar. 2022Industrial Microbiology (Lecture)Spring, 農学研究科
- From Apr. 2021, To Mar. 2022Experimental Course of Fermentation Physiology and Applied Microbiology1Year-long, 農学研究科
- From Apr. 2021, To Mar. 2022Experimental Course of Fermentation Physiology and Applied Microbiology2Year-long, 農学研究科
- From Apr. 2021, To Mar. 2022Fermentation Physiology and Applied Microbiology Seminar1Year-long, 農学研究科
- From Apr. 2021, To Mar. 2022Fermentation Physiology and Applied Microbiology Seminar2Year-long, 農学研究科
