Journal of Bioscience and Bioengineering, 2022年06月
Enzymatic Synthesis of l-threo-β-Hydroxy-α-Amino Acids via Asymmetric Hydroxylation Using 2-Oxoglutarate-Dependent Hydroxylase from Sulfobacillus thermotolerans Y0017.
Ryotaro Hara; Yuta Nakajima; Hiroaki Yanagawa; Ryo Gawasawa; Izumi Hirasawa; Kuniki Kino
Applied and environmental microbiology, 2021年08月04日
A three-component monooxygenase from Rhodococcus wratislaviensis may expand industrial applications of bacterial enzymes.
Applied and Environmental Microbiology, 2019年04月19日, 査読有り
A chemoenzymatic process for amide bond formation by an adenylating enzyme-mediated mechanism
Ryotaro Hara; Kengo Hirai; Shin Suzuki; Kuniki Kino
Scientific Reports, 2018年12月01日, 査読有り
Production of aminoacyl prolines using the adenylation domain of nonribosomal peptide synthetase with class III polyphosphate kinase 2-mediated ATP regeneration
Shin Suzuki; Ryotaro Hara; Kuniki Kino
Journal of Bioscience and Bioengineering, 2018年06月01日, 査読有り
Discovery of lysine hydroxylases in the clavaminic acid synthase-like superfamily for efficient hydroxylysine bioproduction
Ryotaro Hara; Kai Yamagata; Ryoma Miyake; Hiroshi Kawabata; Hisatoshi Uehara; Kuniki Kino
Applied and Environmental Microbiology, 2017年09月01日, 査読有り
Functional characterization of aconitase X as a cis-3-hydroxy-L-proline dehydratase
Seiya Watanabe; Kunihiko Tajima; Satoshi Fujii; Fumiyasu Fukumori; Ryotaro Hara; Rio Fukuda; Mao Miyazaki; Kuniki Kino; Yasuo Watanabe
Scientific Reports, 2016年12月08日, 査読有り
Development of a multi-enzymatic cascade reaction for the synthesis of trans-3-hydroxy-l-proline from l-arginine
Ryotaro Hara; Saki Kitatsuji; Kai Yamagata; Kuniki Kino
Applied Microbiology and Biotechnology, 2016年01月01日, 査読有り
One-Pot Production of l-threo-3-Hydroxyaspartic Acid Using Asparaginase-Deficient Escherichia coli Expressing Asparagine Hydroxylase of Streptomyces coelicolor A3(2)
Ryotaro Hara
Applied and Environmental Microbiology, 2015年06月01日, 査読有り
Hydroxamate-based colorimetric assay to assess amide bond formation by adenylation domain of nonribosomal peptide synthetases
Ryotaro Hara
Analytical Biochemistry, 2015年05月, 査読有り
Refined regio- and stereoselective hydroxylation of l -pipecolic acid by protein engineering of l -proline cis -4-hydroxylase based on the X-ray crystal structure
Identification and characterization of 2-oxoglutarate-dependent dioxygenases catalyzing selective cis-hydroxylation of proline and pipecolinic acid from actinomycetes
Ryotaro Hara
Journal of Biotechnology, 2014年02月, 査読有り
Regio- and stereoselective oxygenation of proline derivatives by using microbial 2-oxoglutarate-dependent dioxygenases
Ryotaro Hara; Naoko Uchiumi; Naoko Okamoto; Kuniki Kino
Bioscience, Biotechnology and Biochemistry, 2014年, 査読有り
Microbial production of N-acetyl cis-4-hydroxy-l-proline by coexpression of the Rhizobium l-proline cis-4-hydroxylase and the yeast N-acetyltransferase Mpr1
Thi Mai Hoa Bach; Ryotaro Hara; Kuniki Kino; Iwao Ohtsu; Nobuyuki Yoshida; Hiroshi Takagi
Applied Microbiology and Biotechnology, 2013年01月, 査読有り
Enhanced synthesis of 5-hydroxy-l-tryptophan through tetrahydropterin regeneration
Ryotaro Hara
AMB Express, 2013年, 査読有り
Industrial Production of threo-3-Hydroxy-L-Aspartic Acid Using Escherichia coli Resting Cells
Ryotaro Hara
Journal of Biotechnology, 2010年11月, 査読有り
Enhancement of l-tryptophan 5-hydroxylation activity by structure-based modification of l-phenylalanine 4-hydroxylase from Chromobacterium violaceum
Kuniki Kino; Ryotaro Hara; Ai Nozawa
Journal of Bioscience and Bioengineering, 2009年09月, 査読有り
Characterization of novel 2-oxoglutarate dependent dioxygenases converting l-proline to cis-4-hydroxy-l-proline
Ryotaro Hara; Kuniki Kino
Biochemical and Biophysical Research Communications, 2009年02月20日, 査読有り
Two groups of thermophilic amino acid aminotransferases exhibiting broad substrate specificities for the synthesis of phenylglycine derivatives
Daisuke Koma; Toshiya Sawai; Ryotaro Hara; Shigeaki Harayama; Kuniki Kino
Applied Microbiology and Biotechnology, 2008年07月, 査読有り
Two groups of thermophilic amino acid aminotransferases exhibiting broad substrate specificities for the synthesis of phenylglycine derivatives
Ryotaro Hara
Applied Microbiology and Biotechnology, 2008年07月, 査読有り
A high-throughput and generic assay method for the determination of substrate specificities of thermophilic α-aminotransferases
4Dp10 Microbial production of N-acetyl cis-4-hydroxy-L-proline by coexpression of the Rhizobium L-proline cis-4-hydroxylase and the yeast N-acetyltranferase Mpr1 :
Hoa Bach Thi Mai; Hara Ryotaro; Kino Kuniki; Takagi Hiroshi