Basic Information

Faculty

• 医学部

Academic Degree

• 修士（理学）(東北大学)
• 博士（理学）(東北大学)

Academic Resume (Graduate Schools)

• 東北大学, 大学院理学研究科博士課程前期２年の課程物理学専攻, 修了
• 東北大学, 大学院理学研究科博士課程後期３年の課程物理学専攻, 修了

Academic Resume (Undergraduate School/Majors)

• 東北大学, 理学部物理学科, 卒業

Research History

• From Feb. 2015, To Present

  Kyoto University, Graduate School of Medicine, 准教授
• From Jul. 2014, To Jan. 2015

  Kyoto University, Graduate School of Medicine, 助教
• From Apr. 2011, To Jun. 2014

  Tohoku University Graduate School of Life Sciences, Department of Biomolecular Sciences, Division of Molecular Biosystems,Laboratory of Single-Molecule Cell Biology, Assistant Professor
• From Mar. 2008, To Mar. 2011

  King's College London, Richard Dimbleby Department of Cancer Research, Randall Division of Cell and Molecular Biophysics, Postdoctoral Research Associate
• From Apr. 2006, To Feb. 2008

  Tohoku University, Graduate School of Medicine, COEフェロー
• From Jul. 2004, To Mar. 2006

  Tohoku University, Graduate School of Life Sciences, 博士研究員

ID,URL

• J-Global ID

  201101056044842027

• Researcher number

  70443984

• External link

  http://db.tohoku.ac.jp/whois/detail/7336f7d5c7002c215832c0ca53ef05c9.html

Website(s) (URL(s))

• http://www.med.kyoto-u.ac.jp/organization-staff/research/doctoral_course/r-020/

researchmap URL

• https://researchmap.jp/read0156360

Research

Research Interests

• 細胞内シグナル伝達
• ErbB
• コフィリン
• アクチン
• 細胞運動
• 細胞骨格
• 超解像顕微鏡法

Research Areas

• Life sciences, Biophysics
• Life sciences, Cell biology

Papers

• Convection-Induced Biased Distribution of Actin Probes in Live Cells

  Yamashiro S; Taniguchi D; Tanaka S; Kiuchi T; Vavylonis D; Watanabe N

  Biophysical Journal, Jan. 2019, Peer-reviewed

  
• Multitarget super-resolution microscopy with high-density labeling by exchangeable probes

  Tai Kiuchi; Makio Higuchi; Akihiro Takamura; Masahiro Maruoka; Naoki Watanabe

  NATURE METHODS, Aug. 2015, Peer-reviewed

  
• The ErbB4 CYT2 variant protects EGFR from ligand-induced degradation to enhance cancer cell motility

  Tai Kiuchi; Elena Ortiz-Zapater; James Monypenny; Daniel R. Matthews; Lan K. Nguyen; Jody Barbeau; Oana Coban; Katherine Lawler; Brian Burford; Daniel J. Rolfe; Emanuele de Rinaldis; Dimitra Dafou; Michael A. Simpson; Natalie Woodman; Sarah Pinder; Cheryl E. Gillett; Viviane Devauges; Simon P. Poland; Gilbert Fruhwirth; Pierfrancesco Marra; Ykelien L. Boersma; Andreas Plueckthun; William J. Gullick; Yosef Yarden; George Santis; Martyn Winn; Boris N. Kholodenko; Marisa L. Martin-Fernandez; Peter Parker; Andrew Tutt; Simon M. Ameer-Beg; Tony Ng

  SCIENCE SIGNALING, Aug. 2014, Peer-reviewed

  
• New single-molecule speckle microscopy reveals modification of the retrograde actin flow by focal adhesions at nanometer scales

  Sawako Yamashiro; Hiroaki Mizuno; Matthew B. Smith; Gillian L. Ryan; Tai Kiuchi; Dimitrios Vavylonis; Naoki Watanabe

  MOLECULAR BIOLOGY OF THE CELL, Apr. 2014, Peer-reviewed

  
• CaMKII beta-mediated LIM-kinase activation plays a crucial role in BDNF-induced neuritogenesis

  Akihiko Saito; Ken Miyajima; Junichi Akatsuka; Hiroshi Kondo; Toshiya Mashiko; Tai Kiuchi; Kazumasa Ohashi; Kensaku Mizuno

  GENES TO CELLS, Jul. 2013, Peer-reviewed

  
• Molecular viewing of actin polymerizing actions and beyond: Combination analysis of single-molecule speckle microscopy with modeling, FRAP and s-FDAP (sequential fluorescence decay after photoactivation)

  Naoki Watanabe; Sawako Yamashiro; Dimitrios Vavylonis; Tai Kiuchi

  Development Growth and Differentiation, May 2013, Peer-reviewed, Invited

  
• F-and G-actin homeostasis regulates mechanosensitive actin nucleation by formins

  Chiharu Higashida; Tai Kiuchi; Yushi Akiba; Hiroaki Mizuno; Masahiro Maruoka; Shuh Narumiya; Kensaku Mizuno; Naoki Watanabe

  Nature Cell Biology, Apr. 2013, Peer-reviewed

  
• Distributed actin turnover in the lamellipodium and FRAP kinetics

  Matthew B. Smith; Tai Kiuchi; Naoki Watanabe; Dimitrios Vavylonis

  Biophysical Journal, 08 Jan. 2013, Peer-reviewed

  
• Visualization of cofilin-actin and Ras-Raf interactions by bimolecular fluorescence complementation assays using a new pair of split Venus fragments

  Kazumasa Ohashi; Tai Kiuchi; Kazuyasu Shoji; Kaori Sampei; Kensaku Mizuno

  BIOTECHNIQUES, Jan. 2012, Peer-reviewed

  
• LIM Kinase Has a Dual Role in Regulating Lamellipodium Extension by Decelerating the Rate of Actin Retrograde Flow and the Rate of Actin Polymerization

  Kazumasa Ohashi; Sachiko Fujiwara; Takuya Watanabe; Hiroshi Kondo; Tai Kiuchi; Masaaki Sato; Kensaku Mizuno

  JOURNAL OF BIOLOGICAL CHEMISTRY, Oct. 2011, Peer-reviewed

  
• Live-cell imaging of G-actin dynamics using sequential FDAP.

  Kiuchi T; Nagai T; Ohashi K; Watanabe N; Mizuno K

  Bioarchitecture, Sep. 2011, Peer-reviewed, Invited

  
• Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension

  Tai Kiuchi; Tomoaki Nagai; Kazumasa Ohashi; Kensaku Mizuno

  JOURNAL OF CELL BIOLOGY, Apr. 2011, Peer-reviewed

  
• LIM-kinase-mediated cofilin phosphorylation decelerates actin retrograde flow in lamellipodia

  K. Ohashi; S. Fujiwara; T. Watanabe; T. Kiuchi; M. Sato; K. Mizuno

  Journal of Biological Chemistry, 2011, Peer-reviewed

  
• The challenges of integrating molecular imaging into the optimization of cancer therapy

  G. S. Patel; T. Kiuchi; K. Lawler; E. Ofo; G. O. Fruhwirth; M. Kelleher; E. Shamil; R. Zhang; P. R. Selvin; G. Santis; J. Spicer; N. Woodman; C. E. Gillett; P. R. Barber; B. Vojnovic; G. Keri; T. Schaeffter; V. Goh; M. J. O'Doherty; P. A. Ellisb; T. Ng

  INTEGRATIVE BIOLOGY, 2011, Peer-reviewed, Invited

  
• The SUMO modification pathway is involved in the BRCA1 response to genotoxic stress

  Joanna R. Morris; Chris Boutell; Melanie Keppler; Ruth Densham; Daniel Weekes; Amin Alamshah; Laura Butler; Yaron Galanty; Laurent Pangon; Tai Kiuchi; Tony Ng; Ellen Solomon

  NATURE, Dec. 2009, Peer-reviewed

  
• An Unbiased Screen Identifies DEP-1 Tumor Suppressor as a Phosphatase Controlling EGFR Endocytosis

  Gabi Tarcic; Shlomit K. Boguslavsky; Jean Wakim; Tai Kiuchi; Angela Liu; Felicia Reinitz; David Nathanson; Takamune Takahashi; Paul S. Mischel; Tony Ng; Yosef Yarden

  CURRENT BIOLOGY, Nov. 2009, Peer-reviewed

  
• Analysis of the regulation of actin cytoskeleton dynamics using dronpa, a photochromic fluorescent protein

  Kazumasa Ohashi; Tai Kiuchi; Tomoaki Nagai; Kensaku Mizuno

  2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, 2008, Peer-reviewed

  
• Cofilin promotes stimulus-induced lamellipodium formation by generating an abundant supply of actin monomers

  Tai Kiuchi; Kazumasa Ohashi; Souichi Kurita; Kensaku Mizuno

  JOURNAL OF CELL BIOLOGY, May 2007, Peer-reviewed

  

Misc.

• 多重染色・高密度標識で従来法の壁を越えるIRIS超解像蛍光顕微鏡法

  渡邊 直樹; 木内 泰

  生体の科学, Oct. 2017, Invited

  
• 細胞生物学とイメージング技術の共鳴誘導 高密度・多重染色超解像顕微鏡法IRISの3次元イメージングへの発展

  木内 泰; 渡邊 直樹

  日本細胞生物学会大会講演要旨集, May 2017

  
• 分子から個体まで神経系を見る 分子の窓を通して覗いた細胞の構造としくみ

  渡邊 直樹; 木内 泰; 山城 佐和子

  バイオイメージング, Aug. 2016

  
• 超解像顕微鏡法IRISの開発による無制限多重染色と高精細な画質の実現

  木内 泰; 渡邊 直樹

  感染・炎症・免疫, Jul. 2016, Invited

  
• 高密度・多重染色超解像蛍光顕微鏡法IRISの原理と実践

  木内 泰; 渡邊 直樹

  生体の科学, Jun. 2016, Invited

  
• 細胞生物学に役に立つ最新技術の展開 多重高密度標識超解像顕微鏡IRISとその応用

  渡邊 直樹; 三好 拓志; 三宅 崇文; 佐々木 瞭; 木内 泰

  日本細胞生物学会大会講演要旨集, May 2016

  
• Multitarget super-resolution microscopy with high-density labeling by exchangeable probes (vol 12, pg 743, 2015)

  Tai Kiuchi; Makio Higuchi; Akihiro Takamura; Masahiro Maruoka; Naoki Watanabe

  NATURE METHODS, Sep. 2015

  
• 蛍光単分子可視化と他の分子動態解析法の融合による細胞内アクチン重合機構の解明

  渡邊 直樹; 木内 泰

  顕微鏡, Jun. 2013, Invited

  
• Dronpaを用いたアクチン動態のライブイメージング

  木内 泰; 水野健作

  実験医学増刊, Oct. 2008, Invited

  
• Slingshot regulates primary dendrite formation in rat hippocampal neurons

  Junichi Akatsuka; Tai Kiuchi; Mitsuharu Endo; Kazumasa Ohashi; Kensaku Mizuno

  NEUROSCIENCE RESEARCH, 2007

  

Industrial Property Rights

• 特許US 10,858,688 B2, 特願15/696,089, OBSERVATION METHOD USING BINDING AND DISSOCIATION PROBE

  KIUCHI Tai; Watanabe Naoki

  
• 特許6422172, 結合解離プローブを用いた観察方法

  木内 泰; 渡邊 直樹; 三好 拓志; 佐々木 瞭

  
• 特願16761865.1, OBSERVATION METHOD USING BOND DISSOCIATION PROBE

  KIUCHI Tai; Watanabe Naoki; MIYOSHI Takushi; SASAKI Ryo

  

Awards

• 26 Feb. 2016

  中谷医工計測技術振興財団, 中谷賞奨励賞

External funds: Kakenhi

• エンドサイトーシスを引き起こす内在性分子群の多色超解像マップの作成

  Grant-in-Aid for Scientific Research (C)

  Basic Section 44010:Cell biology-related

  Kyoto University

  木内 泰

  From 01 Apr. 2021, To 31 Mar. 2024, Granted

  超解像イメージング;エンドサイトーシス;EGF受容体

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K06168/
• 多重染色超解像顕微鏡法による細胞内シグナル伝達の空間マップの作成

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Complex systems

  Kyoto University

  木内 泰

  From 01 Apr. 2019, To 31 Mar. 2021, Project Closed

  細胞内シグナル伝達;超解像イメージング;細胞骨格;EGF受容体;超解像顕微鏡法

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-19H04961/
• The spatial patterning mechanisms of cytoskeletons during cancer cell invasion analyzed by 3D multitarget super-resolution imaging

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Tai Kiuchi

  From 01 Apr. 2017, To 31 Mar. 2020, Project Closed

  超解像顕微鏡法;アクチン;細胞骨格;３次元イメージング;がん浸潤;アクチン骨格;細胞移動

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K07384/
• 細胞内でのタンパク質複合体の多重染色超解像イメージング

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Complex systems

  Kyoto University

  木内 泰

  From 01 Apr. 2015, To 31 Mar. 2017, Project Closed

  超解像顕微鏡;タンパク質複合体;超解像顕微鏡法;細胞骨格;接着斑

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-15H01635/
• The spatial patterning mechanisms of cytoskeletons and focal adhesions analyzed by multitarget super-resolution microscopy

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Tai Kiuchi

  From 01 Apr. 2014, To 31 Mar. 2017, Project Closed

  細胞骨格;超解像顕微鏡;超解像顕微鏡法;接着斑;超解像イメージング;アクチン;微小管;中間径フィラメント

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26440091/
• Self-assembly mechanism for rearrangement of actin cytoskeleton in cell polarization

  Grant-in-Aid for Young Scientists (B)

  Tohoku University

  TAI KIUCHI

  From 01 Apr. 2012, To 31 Mar. 2014, Project Closed

  バイオイメージング;アクチン細胞骨格;アクチンダイナミクス;アクチン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24770146/

External funds: others

• 高密度・多重染色超解像顕微鏡法IRISに用いる結合解離プローブの開発

  GAPファンドプログラム

  From 01 Dec. 2016, To 30 Nov. 2017

  代表

Administration

Faculty management (title, position)

• From 01 Apr. 2015, To 31 Mar. 2019

  動物実験施設運営委員会
• From 01 Sep. 2017, To 31 Mar. 2019

  医学研究科等安全衛生委員会
• From 01 Apr. 2021, To 31 Mar. 2023

  安全衛生委員会委員