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Sakamoto, Masayuki

Graduate School of Biostudies Associate Professor

Sakamoto, Masayuki
list
    Last Updated :2024/06/28

    Basic Information

    Email Address

    • Email Address

      sakamoto.masayuki.2ekyoto-u.ac.jp

    Professional Memberships

    • THE JAPANESE SOCIETY FOR NEUROCHEMISTRY
    • Society for Neuroscience
    • THE MOLECULAR BIOLOGY SOCIETY OF JAPAN
    • THE JAPAN NEUROSCIENCE SOCIETY

    Academic Degree

    • Biostudies(Kyoto University)
    • Biostudies(Kyoto University)

    Academic Resume (Graduate Schools)

    • 京都大学, 大学院生命科学研究科修士課程高次生命科学専攻, 修了
    • 京都大学, 大学院生命科学研究科博士後期課程高次生命科学専攻, 修了

    Academic Resume (Undergraduate School/Majors)

    • 岐阜薬科大学, 薬学部製造薬学科, 卒業

    High School

    • High School

      六甲高校

    Research History

    • From Apr. 2023, To Present
      Kyoto University, Graduate School of Biostudies, Associate Professor
    • From Apr. 2020, To Mar. 2023
      Kyoto University, Graduate School of Biostudies, Associate Professor
    • From Oct. 2019, To Mar. 2023
      科学技術振興機構, さきがけ研究員(兼任)
    • From Apr. 2016, To Mar. 2020
      The University of Tokyo, Graduate School of Medicine, 助教
    • From Oct. 2014, To Mar. 2016
      Columbia University, Department of Biological Sciences, Postdoctoral Fellow
    • From Oct. 2012, To Sep. 2014
      日本学術振興会 海外特別研究員
    • From Apr. 2012, To Sep. 2012
      Kyoto University, Institute for Virus Research, 博士研究員
    • From Apr. 2010, To Mar. 2012
      日本学術振興会 特別研究員(DC2)

    Language of Instruction

    • Japanese
    • English

    ID,URL

    Website(s) (URL(s))

    researchmap URL

    list
      Last Updated :2024/06/28

      Research

      Research Topics, Overview of the research

      • Research Topics

        ・Development of fluorescent probes for monitoring neural activity.
        ・Dendritic computation of electrical and molecular signaling.
        ・Circuit mechanisms underlying odor-induced behaviors.
      • Overview of the research

        Probing functional neural circuits at high spatial-temporal resolution is crucial to understand how neuronal populations work together to achieve higher brain functions such as learning and memory. We aim to understand these circuit mechanisms with cutting-edge multiphoton imaging and optical control technology.

      Research Interests

      • Protein engineering
      • 2光子励起顕微鏡
      • In vivo imaging
      • オプトジェネティクス
      • 樹状突起スパイン
      • 膜電位イメージング
      • カルシウムイメージング

      Research Areas

      • Natural sciences, Bio-, chemical, and soft-matter physics
      • Life sciences, Neuroscience - general
      • Life sciences, Molecular biology

      Papers

      • Probing neuronal activity with genetically encoded calcium and voltage fluorescent indicators
        Sakamoto M; Yokoyama T
        Neuroscience Research, 2024, Peer-reviewed, Invited, Lead author, Corresponding author
      • The A-kinase anchoring protein Yotiao decrease the ER calcium content by inhibiting the store operated calcium entry
        Liuqing Wang; Jiaxuan Zhang; Wanjie Li; Xiaoyan Zhang; Tatsushi Yokoyama; Masayuki Sakamoto; Youjun Wang
        Cell Calcium, Jul. 2024, Peer-reviewed
      • Genome-wide CRISPR screen identifies AC9 as a key regulator of ER Ca2+homeostasis involved in neuronal differentiation
        Liuqing Wang; Jia Li; Yiping Wang; Ziyi Zhong; Yuqing Wang; Rui Huang; Bingwei Zhang; Panpan Liu; Erkejiang Ye; Ruotong Cao; Sher Ali; Yuepeng Ke; Junjie Yang; Tatsushi Yokoyama; Jin Liu; Xiaoyan Zhang; Masayuki Sakamoto; Lin Sun; Yubin Zhou; Youjun Wang
        bioRxiv, 06 Feb. 2024
      • Detection of Membrane Potential-Dependent Rhodopsin Fluorescence Using Low-Intensity Light Emitting Diode for Long-Term Imaging
        Shiho Kawanishi; Keiichi Kojima; Atsushi Shibukawa; Masayuki Sakamoto; Yuki Sudo
        ACS Omega, 25 Jan. 2023, Peer-reviewed
      • A multicolor suite for deciphering population coding of calcium and cAMP in vivo
        Tatsushi Yokoyama; Satoshi Manita; Hiroyuki Uwamori; Mio Tajiri; Itaru Imayoshi; Sho Yagishita; Masanori Murayama; Kazuo Kitamura; Masayuki Sakamoto
        Nature Methods, 21 Mar. 2024, Peer-reviewed, Last author, Corresponding author
      • In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice
        Masayuki Sakamoto; Keisuke Ota; Yayoi Kondo; Michiko Okamura; Hajime Fujii; Haruhiko Bito
        STAR Protocols, Jun. 2022, Peer-reviewed, Lead author, Corresponding author
      • A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo
        Masayuki Sakamoto; Masatoshi Inoue; Atsuya Takeuchi; Shigetaka Kobari; Tatsushi Yokoyama; Shin-ichiro Horigane; Sayaka Takemoto-Kimura; Manabu Abe; Kenji Sakimura; Masanobu Kano; Kazuo Kitamura; Hajime Fujii; Haruhiko Bito
        Cell Reports Methods, Feb. 2022, Peer-reviewed, Lead author, Corresponding author
      • Imaging Voltage with Microbial Rhodopsins
        Xiao Min Zhang; Tatsushi Yokoyama; Masayuki Sakamoto
        Frontiers in Molecular Biosciences, 25 Aug. 2021, Peer-reviewed, Last author, Corresponding author
      • Comparative Studies of the Fluorescence Properties of Microbial Rhodopsins: Spontaneous Emission Versus Photointermediate Fluorescence.
        Keiichi Kojima*; Rika Kurihara*; Masayuki Sakamoto*(Co-first); Tsukasa Takanashi; Hikaru Kuramochi; Xiao Min Zhang; Haruhiko Bito; Tahei Tahara; Yuki Sudo
        The journal of physical chemistry. B, 27 Aug. 2020, Peer-reviewed, Lead author
      • Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics.
        Masatoshi Inoue; Atsuya Takeuchi; Satoshi Manita; Shin-Ichiro Horigane; Masayuki Sakamoto; Ryosuke Kawakami; Kazushi Yamaguchi; Kouhei Otomo; Hiroyuki Yokoyama; Ryang Kim; Tatsushi Yokoyama; Sayaka Takemoto-Kimura; Manabu Abe; Michiko Okamura; Yayoi Kondo; Sean Quirin; Charu Ramakrishnan; Takeshi Imamura; Kenji Sakimura; Tomomi Nemoto; Masanobu Kano; Hajime Fujii; Karl Deisseroth; Kazuo Kitamura; Haruhiko Bito
        Cell, 16 May 2019, Peer-reviewed
      • Comparative evaluation of genetically encoded voltage indicators
        Bando, Y; Sakamoto M; corresponding author; Kim S; Ayzenshtat I; Yuste R
        Cell Reports, Jan. 2019, Peer-reviewed, Lead author, Corresponding author
      • Attenuation of Synaptic Potentials in Dendritic Spines
        Kwon T, Sakamoto M (Co-first author), Peterka D, Yuste R
        Cell Reports, Aug. 2017, Peer-reviewed, Lead author
      • Calmodulin kinases: essential regulators in health and disease
        Takemoto-Kimura S, Suzuki K, Horigane S, Kamijo S, Inoue M, Sakamoto M, Fujii H, Bito H
        Journal of Neurochemistry, Jun. 2017, Peer-reviewed
      • Continuous Postnatal Neurogenesis Contributes to Formation of the Olfactory Bulb Neural Circuits and Flexible Olfactory Associative Learning
        Sakamoto M, Ieki N, Miyoshi G, Mochimaru D, Miyachi H, Imura T, Yamaguchi M, Fishell G, Mori K, Kageyama R, Imayoshi I
        Journal of Neuroscience, Apr. 2014, Peer-reviewed, Lead author
      • The functional significance of newly born neurons integrated into olfactory bulb circuits
        Masayuki Sakamoto; Ryoichiro Kageyama; Itaru Imayoshi
        Frontiers in Neuroscience, 2014, Peer-reviewed, Lead author
      • Genetic visualization of notch signaling in mammalian neurogenesis
        Itaru Imayoshi; Hiromi Shimojo; Masayuki Sakamoto; Toshiyuki Ohtsuka; Ryoichiro Kageyama
        CELLULAR AND MOLECULAR LIFE SCIENCES, Jun. 2013, Peer-reviewed
      • Light-induced silencing of neural activity in Rosa26 knock-in mice conditionally expressing the microbial halorhodopsin eNpHR2.0
        Itaru Imayoshi; Sawako Tabuchi; Kyoko Hirano; Masayuki Sakamoto; Satsuki Kitano; Hitoshi Miyachi; Akihiro Yamanaka; Ryoichiro Kageyama
        Neuroscience Research, Jan. 2013, Peer-reviewed
      • A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination
        Itaru Imayoshi; Kyoko Hirano; Masayuki Sakamoto; Goichi Miyoshi; Tetsuya Imura; Satsuki Kitano; Hitoshi Miyachi; Ryoichiro Kageyama
        Neuroscience Research, May 2012, Peer-reviewed
      • The role of neurogenesis in olfaction-dependent behaviors
        Ryoichiro Kageyama; Itaru Imayoshi; Masayuki Sakamoto
        Behavioural Brain Research, Feb. 2012, Peer-reviewed
      • Continuous neurogenesis in the adult forebrain is required for innate olfactory responses
        Masayuki Sakamoto; Itaru Imayoshi; Toshiyuki Ohtsuka; Masahiro Yamaguchi; Kensaku Mori; Ryoichiro Kageyama
        Proceedings of the National Academy of Science, May 2011, Peer-reviewed, Lead author
      • Genetic Methods to Identify and Manipulate Newly Born Neurons in the Adult Brain
        Itaru Imayoshi; Masayuki Sakamoto; Ryoichiro Kageyama
        Frontiers in Neuroscience, 2011, Peer-reviewed
      • Essential Roles of Notch Signaling in Maintenance of Neural Stem Cells in Developing and Adult Brains
        Itaru Imayoshi; Masayuki Sakamoto; Masahiro Yamaguchi; Kensaku Mori; Ryoichiro Kageyama
        Journal of Neuroscience, Mar. 2010, Peer-reviewed
      • 成体脳ニューロン新生の分子メカニズムと機能的意義
        今吉格; 坂本雅行; 影山龍一郎
        実験医学、28:823-829. 2010., 2010
      • Continuous neurogenesis in the adult brain
        Itaru Imayoshi; Masayuki Sakamoto; Toshiyuki Ohtsuka; Ryoichiro Kageyama
        Development Growth & Differentiation, Apr. 2009, Peer-reviewed
      • 海馬ニューロンの新生―精神疾患と神経機能への関与 03 成体脳ニューロン新生と高次脳機能
        今吉格; 坂本雅行; 影山龍一郎
        メディカルバイオ, 01 Mar. 2009, Peer-reviewed
      • Roles of continuous neurogenesis in the structural and functional integrity of the adult forebrain
        Itaru Imayoshi; Masayuki Sakamoto; Toshiyuki Ohtsuka; Keizo Takao; Tsuyoshi Miyakawa; Masahiro Yamaguchi; Kensaku Mori; Toshio Ikeda; Shigeyoshi Itohara; Ryoichiro Kageyama
        Nature Neuroscience, Oct. 2008, Peer-reviewed

      Misc.

      • イメージング技術を応用した神経活動計測
        坂本雅行
        日本神経回路学会誌, Dec. 2023
      • カルシウムイメージングによる神経活動の計測
        坂本 雅行
        BRAIN and NERVE, Feb. 2023
      • 新規高性能カルシウムセンサー分子設計から脳神経回路の情報動態解明へ
        井上昌俊; 藤井哉; 坂本雅行; 尾藤晴彦
        生体の科学, 2022
      • イメージング技術を用いた神経細胞の電気的活動の計測
        坂本雅行
        生物工学会誌, Jul. 2022
      • 光技術で明らかになった樹状突起スパインの新たな機能
        坂本雅行
        実験医学 2022年5月号, May 2022

      Presentations

      • 神経活動・細胞内シグナル可視化のための 蛍光プローブの開発と応用
        坂本雅行
        第5回神経計算セミナー、同志社大学大学院脳科学研究科(京田辺), 17 Apr. 2024, Invited
      • Fluorescent indicators for probing neuronal activity and biochemical signaling in vivo
        坂本雅行
        The 62nd Frontier Brain Science Seminar, 12 Jan. 2024, Invited
      • 神経活動可視化のための 蛍光プローブの開発と応用
        坂本 雅行
        第5回これからの神経回路研究の会、理化学研究所(神戸), 24 Mar. 2023, Invited
      • Fluorescent biosensors for probing neuronal activity and biochemical signaling
        Masayuki Sakamoto
        Hamburg University - Kyoto University Joint Symposium “Cellular mechanisms of learning and memory”, Kyoto, Japan, 14 Mar. 2023, Invited
      • 神経活動可視化のための蛍光プローブの開発と応用
        坂本 雅行
        第 11 回パラレル脳研究部門 セミナー、東京理科大学 (柏), 09 Mar. 2023, Invited
      • Fluorescent biosensors for probing neuronal activity and signaling
        坂本 雅行
        Cell Biology, Developmental Biology, and Systems Biology Course Meeting, 京都大学(京都), 31 Oct. 2022, Invited
      • Imaging voltage and biochemical signaling in vivo
        Masayuki Sakamoto
        Merocyanine 540/Flash meeting, Marine Biological Laboratory, U.S., 26 Aug. 2022, Invited
      • 膜電位感受性プローブを用いた神経活動計測
        坂本 雅行
        第42 回生体膜と薬物の相互作用シンポジウム(オンライン), 28 Oct. 2021, Invited
      • 合理的な膜電位プローブの設計と応用
        坂本 雅行
        第64回日本神経化学会大会(オンライン), 30 Sep. 2021, Invited
      • Engineering microbial rhodopsins for genetically encoded voltage indicators
        Masayuki Sakamoto
        第44回日本神経科学学会(神戸), 28 Jul. 2021, Invited
      • Genetically encoded voltage indicators and their application
        Masayuki Sakamoto
        遺伝研研究会「哺乳類脳の機能的神経回路の構築メカニズム」(オンライン), 12 Dec. 2020, Invited
      • Imaging voltage in neurons with genetically encoded indicators
        Sakamoto Masayuki
        生理研 記憶研究会(オンライン), 17 Sep. 2020, Invited
      • Imaging voltage in dendritic spines with genetically encoded indicators
        Sakamoto M
        IBRO 2019, Daegu, Korea, 21 Sep. 2019, Invited
      • Genetically encoded voltage indicators and their application
        Sakamoto M
        Neuro2019, 26 Jul. 2019, Invited
      • 膜電位感受性蛍光プローブを用いた神経活動イメージング
        坂本雅行
        シンポジウム「分子の力で生体を視る」、名古屋大学(名古屋), 13 Mar. 2019, Invited
      • 膜電位感受性蛍光プローブを用いた神経活動イメージング
        第1回武田神経科学シンポジウム、武田薬品工業研修所(吹田), 10 Mar. 2018, Invited
      • 膜電位感受性蛍光タンパク質を用いた活動イメージング
        坂本雅行
        第7回生体物理化学セミナー、岡山大学(岡山), 08 Dec. 2016

      Awards

      • 06 Nov. 2020
        コニカミノルタ科学技術振興財団, コニカミノルタ画像科学奨励賞(優秀賞)

      External funds: Kakenhi

      • Development and application of multiphoton excitation for simultaneous multi-color measurement and manipulation of neural activity
        Grant-in-Aid for Challenging Research (Exploratory)
        Medium-sized Section 51:Brain sciences and related fields
        Kyoto University
        Masayuki Sakamoto
        From 09 Jul. 2021, To 31 Mar. 2023, Project Closed
        カルシウムセンサー;光遺伝学ツール;タンパク質工学;多色イメージング;多光子励起;カルシウムイメージング;2光子励起顕微鏡;広視野顕微鏡;FRET;光遺伝学
      • Deciphering functional significance of newly generated neurons at postnatal stage investigated by multi-photon 4D imaging
        Grant-in-Aid for Scientific Research (B)
        Basic Section 59040:Nutrition science and health science-related
        Kyoto University
        Masayuki Sakamoto
        From 01 Apr. 2020, To 31 Mar. 2023, Project Closed
        嗅覚;2光子イメージング;カルシウムセンサー;顆粒細胞;カルシウムイメージング;4Dイメージング;嗅球;高次脳機能;生後発達期
      • 「個性」創発メカニズム解明のためのシナプスイメージング法の開発と応用
        Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
        Complex systems
        Kyoto University;The University of Tokyo
        坂本 雅行
        From 01 Apr. 2019, To 31 Mar. 2021, Project Closed
        「個性」創発;活動イメージング;生後発達期;シナプス
      • Generation and application of transgenic mice for wide-field and multi-color calcium imaging
        Grant-in-Aid for Challenging Research (Exploratory)
        Medium-sized Section 51:Brain sciences and related fields
        The University of Tokyo
        Masayuki Sakamoto
        From 29 Jun. 2018, To 31 Mar. 2020, Project Closed
        カルシウムイメージング;遺伝子改変マウス;2光子励起顕微鏡;多色イメージング;遺伝的改変マウス;2光子励起顕微鏡
      • 「個性」創発メカニズム解明のための樹状突起スパインイメージング法の開発と応用
        Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
        Complex systems
        The University of Tokyo
        坂本 雅行
        From 01 Apr. 2017, To 31 Mar. 2019, Project Closed
        「個性」創発;活動イメージング;生後発達脳;養育行動;樹状突起スパイン
      • 2 光子活動イメージング法を用いた逆転学習を制御する回路メカニズムの解明
        Grant-in-Aid for Research Activity Start-up
        The University of Tokyo
        坂本 雅行
        From 26 Aug. 2016, To 31 Mar. 2018, Granted
        カルシウムイメージング;前頭前皮質;2光子励起顕微鏡;逆転学習;神経科学;イメージング;カルシウムセンサー;眼窩前頭皮質
      • Exploring the functional significance of granule cells in the olfactory bulb using holographic techniques.
        Grant-in-Aid for Scientific Research (B)
        Basic Section 51010:Basic brain sciences-related
        Kyoto University
        坂本 雅行
        From 01 Apr. 2023, To 31 Mar. 2026, Granted
        嗅球;顆粒細胞;カルシウムイメージング;光遺伝学;パターン照射
      • 眠気による細胞内変化可視化のための蛍光プローブ開発
        Grant-in-Aid for Transformative Research Areas (B)
        Transformative Research Areas, Section (III)
        Kyoto University
        坂本 雅行
        From 01 Apr. 2024, To 31 Mar. 2027, Granted
        眠気;蛍光プローブ;2光子励起顕微鏡
      • ホログラム技術を応用した嗅球・顆粒細胞の機能的意義の解明
        Grant-in-Aid for Scientific Research (B)
        Basic Section 51010:Basic brain sciences-related
        Kyoto University
        坂本 雅行
        From 01 Apr. 2024, To 31 Mar. 2026, Granted
        嗅球;顆粒細胞;カルシウムイメージング;光遺伝学;パターン照射
      • 眠気の生成・解消機構の解明のための総括研究
        Grant-in-Aid for Transformative Research Areas (B)
        Transformative Research Areas, Section (III)
        Hirosaki University
        丹羽 康貴
        From 01 Apr. 2024, To 31 Mar. 2027, Granted
        眠気;睡眠行動解析;多光子イメージング;大規模電気生理;数理モデル
      list
        Last Updated :2024/06/28

        Education

        Teaching subject(s)

        • From 01 Apr. 2024, To 31 Mar. 2025
          Introduction to Biology and Life Science
          N484, Spring, Institute for Liberal Arts and Sciences, 2
        • From 01 Apr. 2024, To 31 Mar. 2025
          Mathematical, Statistical and Computational Biology for Expe rimental Biologists
          M344, Year-long, Institute for Liberal Arts and Sciences, 1
        • From 01 Apr. 2024, To 31 Mar. 2025
          Advanced Course in Studies on Signal Transduction
          1029, Spring, Graduate School of Biostudies, 1
        • From 01 Apr. 2024, To 31 Mar. 2025
          Mathematical, Statistical and Computational Biology for Experimental Biologists
          1034, Year-long, Graduate School of Biostudies, 1
        • From 01 Apr. 2024, To 31 Mar. 2025
          Advanced Experiments in Systemic Life Sciences
          5013, Spring, Graduate School of Biostudies, 4
        • From 01 Apr. 2024, To 31 Mar. 2025
          Advanced Experiments in Systemic Life Sciences
          5013, Fall, Graduate School of Biostudies, 4
        • From 01 Apr. 2024, To 31 Mar. 2025
          Experimental Course and Seminar D
          2009, Spring, Graduate School of Biostudies, 5
        • From 01 Apr. 2024, To 31 Mar. 2025
          Experimental Course and Seminar D
          2009, Fall, Graduate School of Biostudies, 5
        • From 01 Apr. 2024, To 31 Mar. 2025
          Experimental Course and Seminar B
          2007, Fall, Graduate School of Biostudies, 5
        • From 01 Apr. 2024, To 31 Mar. 2025
          Experimental Course and Seminar A
          2006, Spring, Graduate School of Biostudies, 5
        • From 01 Apr. 2024, To 31 Mar. 2025
          Experimental Course and Seminar A
          2006, Fall, Graduate School of Biostudies, 5
        • From 01 Apr. 2024, To 31 Mar. 2025
          Experimental Course and Seminar C
          2008, Spring, Graduate School of Biostudies, 5
        • From 01 Apr. 2024, To 31 Mar. 2025
          Experimental Course and Seminar C
          2008, Fall, Graduate School of Biostudies, 5
        • From 01 Apr. 2024, To 31 Mar. 2025
          Experimental Course and Seminar B
          2007, Spring, Graduate School of Biostudies, 5

        Part-time lecturer

        • From 01 Oct. 2023, To 31 Mar. 2024
          富山大学
        list
          Last Updated :2024/06/28

          Academic, Social Contribution

          Committee Memberships

          • From Mar. 2021, To Present
            Frontiers in Behavioral Neuroscience Review editor, Frontiers in Behavioral Neuroscience

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