Basic Information

Email Address

• Email Address

  sakamoto.masayuki.2ekyoto-u.ac.jp

Professional Memberships

• THE JAPANESE SOCIETY FOR NEUROCHEMISTRY
• Society for Neuroscience
• THE MOLECULAR BIOLOGY SOCIETY OF JAPAN
• THE JAPAN NEUROSCIENCE SOCIETY

Academic Degree

• Biostudies(Kyoto University)
• Biostudies(Kyoto University)

Academic Resume (Graduate Schools)

• 京都大学, 大学院生命科学研究科修士課程高次生命科学専攻, 修了
• 京都大学, 大学院生命科学研究科博士後期課程高次生命科学専攻, 修了

Academic Resume (Undergraduate School/Majors)

• 岐阜薬科大学, 薬学部製造薬学科, 卒業

High School

• High School

  六甲高校

Research History

• From Apr. 2023, To Present

  Kyoto University, Graduate School of Biostudies, Associate Professor
• From Apr. 2020, To Mar. 2023

  Kyoto University, Graduate School of Biostudies, Associate Professor
• From Oct. 2019, To Mar. 2023

  科学技術振興機構, さきがけ研究員（兼任）
• From Apr. 2016, To Mar. 2020

  The University of Tokyo, Graduate School of Medicine, 助教
• From Oct. 2014, To Mar. 2016

  Columbia University, Department of Biological Sciences, Postdoctoral Fellow
• From Oct. 2012, To Sep. 2014

  日本学術振興会 海外特別研究員
• From Apr. 2012, To Sep. 2012

  Kyoto University, Institute for Virus Research, 博士研究員
• From Apr. 2010, To Mar. 2012

  日本学術振興会 特別研究員（DC2）

Language of Instruction

• Japanese
• English

ID,URL

• ORCID ID

  0000-0001-6458-113X

• J-Global ID

  201501007912428746

• Researcher number

  00777865

• External link

  https://optoneuro.weebly.com/

Website(s) (URL(s))

• https://optoneuro.weebly.com/

researchmap URL

• https://researchmap.jp/masayukisakamoto

Research

Research Topics, Overview of the research

• Research Topics

  ・Development of fluorescent probes for monitoring neural activity.
  ・Dendritic computation of electrical and molecular signaling.
  ・Circuit mechanisms underlying odor-induced behaviors.

• Overview of the research

  Probing functional neural circuits at high spatial-temporal resolution is crucial to understand how neuronal populations work together to achieve higher brain functions such as learning and memory. We aim to understand these circuit mechanisms with cutting-edge multiphoton imaging and optical control technology.

Research Interests

• Protein engineering
• 2光子励起顕微鏡
• In vivo imaging
• オプトジェネティクス
• 樹状突起スパイン
• 膜電位イメージング
• カルシウムイメージング

Research Areas

• Natural sciences, Bio-, chemical, and soft-matter physics
• Life sciences, Neuroscience - general
• Life sciences, Molecular biology

Papers

• Detection of Membrane Potential-Dependent Rhodopsin Fluorescence Using Low-Intensity Light Emitting Diode for Long-Term Imaging

  Shiho Kawanishi; Keiichi Kojima; Atsushi Shibukawa; Masayuki Sakamoto; Yuki Sudo

  ACS Omega, 25 Jan. 2023, Peer-reviewed

  
• A multicolor suite for deciphering population coding in calcium and cAMP in vivo

  Tatsushi Yokoyama; Satoshi Manita; Hiroyuki Uwamori; Mio Tajiri; Itaru Imayoshi; Sho Yagishita; Masanori Murayama; Kazuo Kitamura; Masayuki Sakamoto

  bioRxiv, 08 Jan. 2023, Last author, Corresponding author

  
• In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice

  Masayuki Sakamoto; Keisuke Ota; Yayoi Kondo; Michiko Okamura; Hajime Fujii; Haruhiko Bito

  STAR Protocols, Jun. 2022, Peer-reviewed, Lead author, Corresponding author

  
• A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo

  Masayuki Sakamoto; Masatoshi Inoue; Atsuya Takeuchi; Shigetaka Kobari; Tatsushi Yokoyama; Shin-ichiro Horigane; Sayaka Takemoto-Kimura; Manabu Abe; Kenji Sakimura; Masanobu Kano; Kazuo Kitamura; Hajime Fujii; Haruhiko Bito

  Cell Reports Methods, Feb. 2022, Peer-reviewed, Lead author, Corresponding author

  
• Imaging Voltage with Microbial Rhodopsins

  Xiao Min Zhang; Tatsushi Yokoyama; Masayuki Sakamoto

  Frontiers in Molecular Biosciences, 25 Aug. 2021, Peer-reviewed, Last author, Corresponding author

  
• Comparative Studies of the Fluorescence Properties of Microbial Rhodopsins: Spontaneous Emission Versus Photointermediate Fluorescence.

  Keiichi Kojima*; Rika Kurihara*; Masayuki Sakamoto*(Co-first); Tsukasa Takanashi; Hikaru Kuramochi; Xiao Min Zhang; Haruhiko Bito; Tahei Tahara; Yuki Sudo

  The journal of physical chemistry. B, 27 Aug. 2020, Peer-reviewed, Lead author

  
• Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of Complex Brain Circuit Dynamics.

  Masatoshi Inoue; Atsuya Takeuchi; Satoshi Manita; Shin-Ichiro Horigane; Masayuki Sakamoto; Ryosuke Kawakami; Kazushi Yamaguchi; Kouhei Otomo; Hiroyuki Yokoyama; Ryang Kim; Tatsushi Yokoyama; Sayaka Takemoto-Kimura; Manabu Abe; Michiko Okamura; Yayoi Kondo; Sean Quirin; Charu Ramakrishnan; Takeshi Imamura; Kenji Sakimura; Tomomi Nemoto; Masanobu Kano; Hajime Fujii; Karl Deisseroth; Kazuo Kitamura; Haruhiko Bito

  Cell, 16 May 2019, Peer-reviewed

  
• Comparative evaluation of genetically encoded voltage indicators

  Bando, Y; Sakamoto M; corresponding author; Kim S; Ayzenshtat I; Yuste R

  Cell Reports, Jan. 2019, Peer-reviewed, Lead author, Corresponding author

  
• Attenuation of Synaptic Potentials in Dendritic Spines

  Kwon T, Sakamoto M (Co-first author), Peterka D, Yuste R

  Cell Reports, Aug. 2017, Peer-reviewed, Lead author

  
• Calmodulin kinases: essential regulators in health and disease

  Takemoto-Kimura S, Suzuki K, Horigane S, Kamijo S, Inoue M, Sakamoto M, Fujii H, Bito H

  Journal of Neurochemistry, Jun. 2017, Peer-reviewed

  
• Continuous Postnatal Neurogenesis Contributes to Formation of the Olfactory Bulb Neural Circuits and Flexible Olfactory Associative Learning

  Sakamoto M, Ieki N, Miyoshi G, Mochimaru D, Miyachi H, Imura T, Yamaguchi M, Fishell G, Mori K, Kageyama R, Imayoshi I

  Journal of Neuroscience, Apr. 2014, Peer-reviewed, Lead author

  
• The functional significance of newly born neurons integrated into olfactory bulb circuits

  Masayuki Sakamoto; Ryoichiro Kageyama; Itaru Imayoshi

  Frontiers in Neuroscience, 2014, Peer-reviewed, Lead author

  
• Genetic visualization of notch signaling in mammalian neurogenesis

  Itaru Imayoshi; Hiromi Shimojo; Masayuki Sakamoto; Toshiyuki Ohtsuka; Ryoichiro Kageyama

  CELLULAR AND MOLECULAR LIFE SCIENCES, Jun. 2013, Peer-reviewed

  
• Light-induced silencing of neural activity in Rosa26 knock-in mice conditionally expressing the microbial halorhodopsin eNpHR2.0

  Itaru Imayoshi; Sawako Tabuchi; Kyoko Hirano; Masayuki Sakamoto; Satsuki Kitano; Hitoshi Miyachi; Akihiro Yamanaka; Ryoichiro Kageyama

  NEUROSCIENCE RESEARCH, Jan. 2013, Peer-reviewed

  
• A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination

  Itaru Imayoshi; Kyoko Hirano; Masayuki Sakamoto; Goichi Miyoshi; Tetsuya Imura; Satsuki Kitano; Hitoshi Miyachi; Ryoichiro Kageyama

  NEUROSCIENCE RESEARCH, May 2012, Peer-reviewed

  
• The role of neurogenesis in olfaction-dependent behaviors

  Ryoichiro Kageyama; Itaru Imayoshi; Masayuki Sakamoto

  BEHAVIOURAL BRAIN RESEARCH, Feb. 2012, Peer-reviewed

  
• Continuous neurogenesis in the adult forebrain is required for innate olfactory responses

  Masayuki Sakamoto; Itaru Imayoshi; Toshiyuki Ohtsuka; Masahiro Yamaguchi; Kensaku Mori; Ryoichiro Kageyama

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, May 2011, Peer-reviewed, Lead author

  
• Genetic methods to identify and manipulate newly born neurons in the adult brain.

  Imayoshi I; Sakamoto M; Kageyama R

  Frontiers in neuroscience, 2011, Peer-reviewed

  
• Essential Roles of Notch Signaling in Maintenance of Neural Stem Cells in Developing and Adult Brains

  Itaru Imayoshi; Masayuki Sakamoto; Masahiro Yamaguchi; Kensaku Mori; Ryoichiro Kageyama

  JOURNAL OF NEUROSCIENCE, Mar. 2010, Peer-reviewed

  
• 成体脳ニューロン新生の分子メカニズムと機能的意義

  今吉格; 坂本雅行; 影山龍一郎

  実験医学、28:823-829. 2010., 2010

  
• Continuous neurogenesis in the adult brain

  Itaru Imayoshi; Masayuki Sakamoto; Toshiyuki Ohtsuka; Ryoichiro Kageyama

  DEVELOPMENT GROWTH & DIFFERENTIATION, Apr. 2009, Peer-reviewed

  
• 海馬ニューロンの新生―精神疾患と神経機能への関与 ０３ 成体脳ニューロン新生と高次脳機能

  今吉格; 坂本雅行; 影山龍一郎

  メディカルバイオ, 01 Mar. 2009, Peer-reviewed

  
• Roles of continuous neurogenesis in the structural and functional integrity of the adult forebrain

  Itaru Imayoshi; Masayuki Sakamoto; Toshiyuki Ohtsuka; Keizo Takao; Tsuyoshi Miyakawa; Masahiro Yamaguchi; Kensaku Mori; Toshio Ikeda; Shigeyoshi Itohara; Ryoichiro Kageyama

  NATURE NEUROSCIENCE, Oct. 2008, Peer-reviewed

  

Misc.

• カルシウムイメージングによる神経活動の計測

  坂本 雅行

  BRAIN and NERVE, Feb. 2023

  
• 革新脳と関連プロジェクトから見えてきた新しい脳科学 II.脳機能マッピングのための新規技術開発 新規高性能カルシウムセンサー分子設計から脳神経回路の情報動態解明へ

  井上昌俊; 藤井哉; 坂本雅行; 尾藤晴彦

  生体の科学, 2022

  
• イメージング技術を用いた神経細胞の電気的活動の計測

  坂本雅行

  生物工学会誌, Jul. 2022

  
• 光技術で明らかになった樹状突起スパインの新たな機能

  坂本雅行

  実験医学 2022年5月号, May 2022

  

Presentations

• 神経活動可視化のための 蛍光プローブの開発と応用

  坂本 雅行

  第５回これからの神経回路研究の会、理化学研究所（神戸）, 24 Mar. 2023, Invited

  
• Fluorescent biosensors for probing neuronal activity and biochemical signaling

  Masayuki Sakamoto

  Hamburg University - Kyoto University Joint Symposium “Cellular mechanisms of learning and memory”, Kyoto, Japan, 14 Mar. 2023, Invited

  
• 神経活動可視化のための蛍光プローブの開発と応用

  坂本 雅行

  第 11 回パラレル脳研究部門 セミナー、東京理科大学 （柏）, 09 Mar. 2023, Invited

  
• Fluorescent biosensors for probing neuronal activity and signaling

  坂本 雅行

  Cell Biology, Developmental Biology, and Systems Biology Course Meeting, 京都大学（京都）, 31 Oct. 2022, Invited

  
• Imaging voltage and biochemical signaling in vivo

  Masayuki Sakamoto

  Merocyanine 540/Flash meeting, Marine Biological Laboratory, U.S., 26 Aug. 2022, Invited

  
• 膜電位感受性プローブを用いた神経活動計測

  坂本 雅行

  第42 回生体膜と薬物の相互作用シンポジウム（オンライン）, 28 Oct. 2021, Invited

  
• 合理的な膜電位プローブの設計と応用

  坂本 雅行

  第64回日本神経化学会大会（オンライン）, 30 Sep. 2021, Invited

  
• Engineering microbial rhodopsins for genetically encoded voltage indicators

  Masayuki Sakamoto

  第44回日本神経科学学会（神戸）, 28 Jul. 2021, Invited

  
• Genetically encoded voltage indicators and their application

  Masayuki Sakamoto

  遺伝研研究会「哺乳類脳の機能的神経回路の構築メカニズム」（オンライン）, 12 Dec. 2020, Invited

  
• Imaging voltage in neurons with genetically encoded indicators

  Sakamoto Masayuki

  生理研 記憶研究会（オンライン）, 17 Sep. 2020, Invited

  
• Imaging voltage in dendritic spines with genetically encoded indicators

  Sakamoto M

  IBRO 2019, Daegu, Korea, 21 Sep. 2019, Invited

  
• Genetically encoded voltage indicators and their application

  Sakamoto M

  Neuro2019, 26 Jul. 2019, Invited

  
• 膜電位感受性蛍光プローブを用いた神経活動イメージング

  坂本雅行

  シンポジウム「分子の力で生体を視る」、名古屋大学（名古屋）, 13 Mar. 2019, Invited

  
• 膜電位感受性蛍光プローブを用いた神経活動イメージング

  第1回武田神経科学シンポジウム、武田薬品工業研修所（吹田）, 10 Mar. 2018, Invited

  
• 膜電位感受性蛍光タンパク質を用いた活動イメージング

  坂本雅行

  第７回生体物理化学セミナー、岡山大学（岡山）, 08 Dec. 2016

  

Awards

• 06 Nov. 2020

  コニカミノルタ科学技術振興財団, コニカミノルタ画像科学奨励賞（優秀賞）

External funds: Kakenhi

• 多光子励起による生体多色活動計測・操作技術の開発と応用

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 51:Brain sciences and related fields

  Kyoto University

  坂本 雅行

  From 09 Jul. 2021, To 31 Mar. 2023, Granted

  光遺伝学ツール;FRET;多色イメージング;光遺伝学;多光子励起

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K19429/
• Deciphering functional significance of newly generated neurons at postnatal stage investigated by multi-photon 4D imaging

  Grant-in-Aid for Scientific Research (B)

  Basic Section 59040:Nutrition science and health science-related

  Kyoto University

  坂本 雅行

  From 01 Apr. 2020, To 31 Mar. 2023, Granted

  カルシウムイメージング;４Dイメージング;嗅球;高次脳機能;生後発達期

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20H04122/
• 「個性」創発メカニズム解明のためのシナプスイメージング法の開発と応用

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Complex systems

  Kyoto University;The University of Tokyo

  坂本 雅行

  From 01 Apr. 2019, To 31 Mar. 2021, Project Closed

  「個性」創発;活動イメージング;生後発達期;シナプス

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-19H04898/
• Generation and application of transgenic mice for wide-field and multi-color calcium imaging

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 51:Brain sciences and related fields

  The University of Tokyo

  Masayuki Sakamoto

  From 29 Jun. 2018, To 31 Mar. 2020, Project Closed

  カルシウムイメージング;遺伝子改変マウス;２光子励起顕微鏡;多色イメージング;遺伝的改変マウス;2光子励起顕微鏡

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K19493/
• 「個性」創発メカニズム解明のための樹状突起スパインイメージング法の開発と応用

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Complex systems

  The University of Tokyo

  坂本 雅行

  From 01 Apr. 2017, To 31 Mar. 2019, Project Closed

  「個性」創発;活動イメージング;生後発達脳;養育行動;樹状突起スパイン

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-17H05941/
• 2 光子活動イメージング法を用いた逆転学習を制御する回路メカニズムの解明

  Grant-in-Aid for Research Activity Start-up

  The University of Tokyo

  坂本 雅行

  From 26 Aug. 2016, To 31 Mar. 2018, Granted

  カルシウムイメージング;前頭前皮質;2光子励起顕微鏡;逆転学習;神経科学;イメージング;カルシウムセンサー;眼窩前頭皮質

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16H06728/
• ホログラム技術を応用した嗅球・顆粒細胞の機能的意義の解明

  Grant-in-Aid for Scientific Research (B)

  Basic Section 51010:Basic brain sciences-related

  Kyoto University

  坂本 雅行

  From 01 Apr. 2023, To 31 Mar. 2026, Adopted

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23H02782/

Academic, Social Contribution

Committee Memberships

• From Mar. 2021, To Present

  Frontiers in Behavioral Neuroscience Review editor, Frontiers in Behavioral Neuroscience