Basic Information

Academic Degree

• 修士（地球環境科学）(北海道大学)
• 博士（地球環境科学）(北海道大学)

ID,URL

• J-Global ID

  201401090445083892

researchmap URL

• https://researchmap.jp/7000009233

Research

Research Topics, Overview of the research

• Research Topics

  Dosage compensation for X-linked genes in primates

• Overview of the research

  In female mammals, including humans (XX), one of the two X chromosomes undergoes coordinated inactivation early in development to balance gene expression with males (XY). This phenomenon is known as X chromosome inactivation (XCI). We are studying the dynamics and mechanisms of XCI in primates using early embryos and embryonic stem cells of crab-eating macaques.

Research Areas

• Life sciences, Molecular biology

Papers

• Generation of functional oocytes from male mice in vitro.

  Kenta Murakami; Nobuhiko Hamazaki; Norio Hamada; Go Nagamatsu; Ikuhiro Okamoto; Hiroshi Ohta; Yoshiaki Nosaka; Yukiko Ishikura; Tomoya S Kitajima; Yuichiro Semba; Yuya Kunisaki; Fumio Arai; Koichi Akashi; Mitinori Saitou; Kiyoko Kato; Katsuhiko Hayashi

  Nature, 15 Mar. 2023, Peer-reviewed

  
• ヒト多能性幹細胞を用いた始原生殖細胞様細胞の試験管内誘導

  横林 しほり; 佐々木 恒太郎; 中村 友紀; 岡本 郁弘; 薮田 幸宏; 栗本 一基; 大田 浩; 守時 良演; 佐々木 裕子; 中川 誠人; 斎藤 通紀

  日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, Dec. 2015

  
• Induction of fetal meiotic oocytes from embryonic stem cells in cynomolgus monkeys

  Sayuri Gyobu‐Motani; Yukihiro Yabuta; Ken Mizuta; Yoshitaka Katou; Ikuhiro Okamoto; Masanori Kawasaki; Ayaka Kitamura; Tomoyuki Tsukiyama; Chizuru Iwatani; Hideaki Tsuchiya; Taro Tsujimura; Takuya Yamamoto; Tomonori Nakamura; Mitinori Saitou

  The EMBO Journal, 16 Mar. 2023, Peer-reviewed

  
• Nucleome programming is required for the foundation of totipotency in mammalian germline development

  Masahiro Nagano; Bo Hu; Shihori Yokobayashi; Akitoshi Yamamura; Fumiya Umemura; Mariel Coradin; Hiroshi Ohta; Yukihiro Yabuta; Yukiko Ishikura; Ikuhiro Okamoto; Hiroki Ikeda; Naofumi Kawahira; Yoshiaki Nosaka; Sakura Shimizu; Yoji Kojima; Ken Mizuta; Tomoko Kasahara; Yusuke Imoto; Killian Meehan; Roman Stocsits; Gordana Wutz; Yasuaki Hiraoka; Yasuhiro Murakawa; Takuya Yamamoto; Kikue Tachibana; Jan‐Michel Peters; Leonid A Mirny; Benjamin A Garcia; Jacek Majewski; Mitinori Saitou

  The EMBO Journal, 04 Jul. 2022, Peer-reviewed

  
• The embryonic ontogeny of the gonadal somatic cells in mice and monkeys.

  Kotaro Sasaki; Akiko Oguchi; Keren Cheng; Yasuhiro Murakawa; Ikuhiro Okamoto; Hiroshi Ohta; Yukihiro Yabuta; Chizuru Iwatani; Hideaki Tsuchiya; Takuya Yamamoto; Yasunari Seita; Mitinori Saitou

  Cell reports, 04 May 2021, Peer-reviewed

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• GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program.

  Yoji Kojima; Chika Yamashiro; Yusuke Murase; Yukihiro Yabuta; Ikuhiro Okamoto; Chizuru Iwatani; Hideaki Tsuchiya; Masataka Nakaya; Tomoyuki Tsukiyama; Tomonori Nakamura; Takuya Yamamoto; Mitinori Saitou

  Life science alliance, May 2021, Peer-reviewed

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• Capturing Human Trophoblast Development with Naive Pluripotent Stem Cells In Vitro

  Shingo Io; Mio Kabata; Yoshiki Iemura; Katsunori Semi; Nobuhiro Morone; Ikuhiro Okamoto; Tomonori Nakamura; Yoji Kojima; Chizuru Iwatani; Hideaki Tsuchiya; Belinda Kaswandy; Eiji Kondoh; Mitinori Saitou; Takuya Yamamoto; Masaki Mandai; Yasuhiro Takashima

  18 Dec. 2020, Peer-reviewed

  
• Induction of the Germ-Cell Fate from Pluripotent Stem Cells in Cynomolgus Monkeys.

  Sakai Y; Nakamura T; Okamoto I; Gyobu-Motani S; Ohta H; Yabuta Y; Tsukiyama T; Iwatani C; Tsuchiya H; Ema M; Morizane A; Takahashi J; Yamamoto T; Saitou M

  Biology of reproduction, Nov. 2019, Peer-reviewed

  
• A symmetric toggle switch explains the onset of random X inactivation in different mammals.

  Mutzel V; Okamoto I; Dunkel I; Saitou M; Giorgetti L; Heard E; Schulz EG

  Nature structural & molecular biology, May 2019, Peer-reviewed

  
• Generation of human oogonia from induced pluripotent stem cells in vitro.

  Yamashiro C; Sasaki K; Yabuta Y; Kojima Y; Nakamura T; Okamoto I; Yokobayashi S; Murase Y; Ishikura Y; Shirane K; Sasaki H; Yamamoto T; Saitou M

  Science (New York, N.Y.), Oct. 2018, Peer-reviewed

  
• Combined Immunofluorescence, RNA FISH, and DNA FISH in Preimplantation Mouse Embryos.

  Okamoto I

  Methods in molecular biology (Clifton, N.J.), 2018, Peer-reviewed, Lead author, Last author, Corresponding author

  
• Contribution of epigenetic landscapes and transcription factors to X-chromosome reactivation in the inner cell mass

  Maud Borensztein; Ikuhiro Okamoto; Laurene Syx; Guillaume Guilbaud; Christel Picard; Katia Ancelin; Rafael Galupa; Patricia Diabangouaya; Nicolas Servant; Emmanuel Barillot; Azim Surani; Mitinori Saitou; Chong-Jian Chen; Konstantinos Anastassiadis; Edith Heard

  NATURE COMMUNICATIONS, Nov. 2017, Peer-reviewed

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• Single-cell transcriptome of early embryos and cultured embryonic stem cells of cynomolgus monkeys

  Tomonori Nakamura; Yukihiro Yabuta; Ikuhiro Okamoto; Kotaro Sasaki; Chizuru Iwatani; Hideaki Tsuchiya; Mitinori Saitou

  Scientific Data, Jun. 2017, Peer-reviewed

  
• In vitro expansion of mouse primordial germ cell-like cells recapitulates an epigenetic blank slate.

  Ohta H; Kurimoto K; Okamoto I; Nakamura T; Yabuta Y; Miyauchi H; Yamamoto T; Okuno Y; Hagiwara M; Shirane K; Sasaki H; Saitou M

  The EMBO journal, May 2017, Peer-reviewed

  
• RNA FISH to study zygotic genome activation in early mouse embryos

  Noémie Ranisavljevic; Ikuhiro Okamoto; Edith Heard; Katia Ancelin

  Methods in Molecular Biology, 2017, Peer-reviewed

  
• In Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells.

  Ishikura Y; Yabuta Y; Ohta H; Hayashi K; Nakamura T; Okamoto I; Yamamoto T; Kurimoto K; Shirane K; Sasaki H; Saitou M

  Cell reports, Dec. 2016, Peer-reviewed

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• The Germ Cell Fate of Cynomolgus Monkeys Is Specified in the Nascent Amnion.

  Sasaki K; Nakamura T; Okamoto I; Yabuta Y; Iwatani C; Tsuchiya H; Seita Y; Nakamura S; Shiraki N; Takakuwa T; Yamamoto T; Saitou M

  Developmental cell, Oct. 2016, Peer-reviewed

  
• A developmental coordinate of pluripotency among mice, monkeys and humans.

  Nakamura T; Okamoto I; Sasaki K; Yabuta Y; Iwatani C; Tsuchiya H; Seita Y; Nakamura S; Yamamoto T; Saitou M

  Nature, Aug. 2016, Peer-reviewed

  
• Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells.

  Sasaki K; Yokobayashi S; Nakamura T; Okamoto I; Yabuta Y; Kurimoto K; Ohta H; Moritoki Y; Iwatani C; Tsuchiya H; Nakamura S; Sekiguchi K; Sakuma T; Yamamoto T; Mori T; Woltjen K; Nakagawa M; Yamamoto T; Takahashi K; Yamanaka S

  Cell stem cell, Aug. 2015, Peer-reviewed

  
• SC3-seq: a method for highly parallel and quantitative measurement of single-cell gene expression.

  Nakamura T; Yabuta Y; Okamoto I; Aramaki S; Yokobayashi S; Kurimoto K; Sekiguchi K; Nakagawa M; Yamamoto T; Saitou M

  Nucleic acids research, May 2015, Peer-reviewed

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• The Gpr1/Zdbf2 locus provides new paradigms for transient and dynamic genomic imprinting in mammals

  Rachel Duffie; Sophie Ajjan; Maxim V. Greenberg; Natasha Zamudio; Martin Escamilla del Arenal; Julian Iranzo; Ikuhiro Okamoto; Sandrine Barbaux; Patricia Fauque; Deborah Bourc'his

  GENES & DEVELOPMENT, Mar. 2014, Peer-reviewed

  
• The two active X chromosomes in female ESCs block exit from the pluripotent state by modulating the ESC signaling network.

  Schulz EG; Meisig J; Nakamura T; Okamoto I; Sieber A; Picard C; Borensztein M; Saitou M; Blüthgen N; Heard E

  Cell Stem Cell, Feb. 2014, Peer-reviewed

  
• RNAi-Dependent and Independent Control of LINE1 Accumulation and Mobility in Mouse Embryonic Stem Cells (Retracted article. See vol. 9, e1005519, 2015)

  Constance Ciaudo; Florence Jay; Ikuhiro Okamoto; Chong-Jian Chen; Alexis Sarazin; Nicolas Servant; Emmanuel Barillot; Edith Heard; Olivier Voinnet

  PLOS GENETICS, Nov. 2013, Peer-reviewed

  
• Initiation of X chromosome inactivation during early embryogenesis in mice and in humans

  Catherine Patrat; Ikuhiro Okamoto; Edith Heard

  BULLETIN DE L ACADEMIE NATIONALE DE MEDECINE, Mar. 2013, Peer-reviewed

  
• Spatial partitioning of the regulatory landscape of the X-inactivation centre

  Elphege P. Nora; Bryan R. Lajoie; Edda G. Schulz; Luca Giorgetti; Ikuhiro Okamoto; Nicolas Servant; Tristan Piolot; Nynke L. van Berkum; Johannes Meisig; John Sedat; Joost Gribnau; Emmanuel Barillot; Nils Bluethgen; Job Dekker; Edith Heard

  NATURE, May 2012, Peer-reviewed

  
• Eutherian mammals use diverse strategies to initiate X-chromosome inactivation during development (vol 472, pg 370, 2011)

  Ikuhiro Okamoto; Catherine Patrat; Dominique Thepot; Nathalie Peynot; Patricia Fauque; Nathalie Daniel; Patricia Diabangouaya; Jean-Philippe Wolf; Jean-Paul Renard; Veronique Duranthon; Edith Heard

  NATURE, Jun. 2011, Peer-reviewed

  
• Eutherian mammals use diverse strategies to initiate X-chromosome inactivation during development

  Ikuhiro Okamoto; Catherine Patrat; Dominique Thepot; Nathalie Peynot; Patricia Fauque; Nathalie Daniel; Patricia Diabangouaya; Jean-Philippe Wolf; Jean-Paul Renard; Veronique Duranthon; Edith Heard

  NATURE, Apr. 2011, Peer-reviewed

  
• A Strand-Specific Burst in Transcription of Pericentric Satellites Is Required for Chromocenter Formation and Early Mouse Development

  Aline V. Probst; Ikuhiro Okamoto; Miguel Casanova; Fatima El Marjou; Patricia Le Baccon; Genevieve Almouzni

  DEVELOPMENTAL CELL, Oct. 2010, Peer-reviewed

  
• Lessons from comparative analysis of X-chromosome inactivation in mammals

  Ikuhiro Okamoto; Edith Heard

  CHROMOSOME RESEARCH, Jul. 2009, Peer-reviewed

  
• Monomethylation of Histone H4-Lysine 20 Is Involved in Chromosome Structure and Stability and Is Essential for Mouse Development

  Hisanobu Oda; Ikuhiro Okamoto; Niall Murphy; Jianhua Chu; Sandy M. Price; Michael M. Shen; Maria Elena Torres-Padilla; Edith Heard; Danny Reinberg

  MOLECULAR AND CELLULAR BIOLOGY, Apr. 2009, Peer-reviewed

  
• The dynamics of imprinted X inactivation during preimplantation development in mice

  I. Okamoto; E. Heard

  CYTOGENETIC AND GENOME RESEARCH, 2006, Peer-reviewed

  
• Evidence for de novo imprinted X-chromosome inactivation independent of meiotic inactivation in mice

  Okamoto, I; D Arnaud; P Le Baccon; AP Otte; CM Disteche; P Avner; E Heard

  NATURE, Nov. 2005, Peer-reviewed

  
• Initiation of epigenetic reprogramming of the X chromosome in somatic nuclei transplanted to a mouse oocyte

  SQ Bao; N Miyoshi; Okamoto, I; T Jenuwein; E Heard; MA Surani

  EMBO REPORTS, Aug. 2005, Peer-reviewed

  
• Imprinted X-inactivation in extra-embryonic endoderm cell lines from mouse blastocysts

  T Kunath; D Arnaud; GD Uy; L Okamoto; C Chureau; Y Yamanaka; E Heard; RL Gardner; P Avner; J Rossant

  DEVELOPMENT, Apr. 2005, Peer-reviewed

  
• Mammalian X-chromosome inactivation: An epigenetics paradigm

  E Heard; J Chaumeil; O Masui; Okamoto, I

  COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 2004, Peer-reviewed

  
• X-chromosome inactivation in mouse embryonic stem cells: Analysis of histone modifications and transcriptional activity using immunofluorescence and FISH

  J Chaumeil; Okamoto, I; E Heard

  CHROMATIN AND CHROMATIN REMODELING ENZYMES, PT B, 2004, Peer-reviewed

  
• Epigenetic dynamics of imprinted X inactivation during early mouse development

  Okamoto, I; AP Otte; CD Allis; D Reinberg; E Heard

  SCIENCE, Jan. 2004, Peer-reviewed

  
• Developmental abnormalities in mouse embryos tetrasomic for chromosome 11: Apparent similarity to embryos functionally disomic for the X chromosome

  H Mizuno; Okamoto, I; N Takagi

  GENES & GENETIC SYSTEMS, Aug. 2002, Peer-reviewed

  
• Integrated kinetics of X chromosome inactivation in differentiating embryonic stem cells

  J Chaumeil; Okamoto, I; M Guggiari; E Heard

  CYTOGENETIC AND GENOME RESEARCH, 2002, Peer-reviewed

  
• X-chromosome inactivation in XX androgenetic mouse embryos surviving implantation

  Okamoto, I; SS Tan; N Takagi

  DEVELOPMENT, Oct. 2000, Peer-reviewed

  
• The X chromosome dosage compensation program during the development of cynomolgus monkeys.

  Ikuhiro Okamoto; Tomonori Nakamura; Kotaro Sasaki; Yukihiro Yabuta; Chizuru Iwatani; Hideaki Tsuchiya; Shin-Ichiro Nakamura; Masatsugu Ema; Takuya Yamamoto; Mitinori Saitou

  Science (New York, N.Y.), 19 Nov. 2021, Peer-reviewed, Lead author, Corresponding author

  

Misc.

• Generation of human oogonia from induced pluripotent stem cells in vitro

  Chika Yamashiro; Kotaro Sasaki; Yukihiro Yabuta; Yoji Kojima; Tomonori Nakamura; Ikuhiro Okamoto; Shihori Yokobayashi; Yusuke Murase; Yukiko Ishikura; Kenjiro Shirane; Hiroyuki Sasaki; Takuya Yamamoto; Mitinori Saitou

  Obstetrical and Gynecological Survey, 01 Mar. 2019

  
• Establishment of macaque trophoblast stem cell lines derived from cynomolgus monkey blastocysts.

  Shoma Matsumoto; Christopher J Porter; Naomi Ogasawara; Chizuru Iwatani; Hideaki Tsuchiya; Yasunari Seita; Yu-Wei Chang; Ikuhiro Okamoto; Mitinori Saitou; Masatsugu Ema; Theodore J Perkins; William L Stanford; Satoshi Tanaka

  Scientific reports, 22 Apr. 2020

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Presentations

• The X-chromosome dosage compensation program during the development of cynomolgus monkeys

  Ikuhiro Okamoto

  X-chromosome inactivation: New insights on its 60th anniversary

  
• カニクイザルの発生過程におけるX染色体遺伝子量補正プログラム

  岡本郁弘

  日本人類遺伝学会第６７大会, 16 Dec. 2022, Invited

  
• The X chromosome dosage compensation program during the development of cynomolgus monkeys

  Ikuhiro Okamoto

  第45回日本分子生物学会年会, 01 Dec. 2022, Invited

  

Books and Other Publications

• X染色体不活化と疾患ー新たな展開

  Joint work, 霊長類におけるX染色体遺伝子量補正

  医歯薬出版株式会社, Nov. 2022

  

External funds: Kakenhi

• Analysis of early post-implantation development and establishment of physiological ex vivo culture method in primate

  Grant-in-Aid for Transformative Research Areas (B)

  Transformative Research Areas, Section (III)

  Kyoto University

  Tomonori Nakamura

  From 02 Oct. 2020, To 31 Mar. 2023, Project Closed

  霊長類;発生学;scRNA-seq;着床後胚発生;次元の呪い;カニクイザル

  https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-20H05761/
• Study of X chromosome inactivation in the monkey embryos

  Grant-in-Aid for Scientific Research (C)

  Basic Section 42040:Laboratory animal science-related

  Kyoto University

  ikuhiro okamoto

  From 01 Apr. 2018, To 31 Mar. 2021, Project Closed

  カニクイザル;X染色体の不活性化;ゲノム編集;ノックアウト細胞;X染色体不活性化;XIST RNA;X染色体の再活性化;初期胚;始原生殖細胞

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K06030/
• The dynamics of X chromosome reactivation in female PGCs

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  ikuhiro okamoto

  From 01 Apr. 2013, To 31 Mar. 2017, Project Closed

  マウス;X染色体;再活性化;始原生殖細胞;始源生殖細胞;不活性X染色体の再活性化;不活性X染色体;リプログラミング;ゲノムリプログラミング

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25291076/
• The study of X chromosome inactivation maintenance in primate.

  Grant-in-Aid for Scientific Research (B)

  Basic Section 42040:Laboratory animal science-related

  Kyoto University

  岡本 郁弘

  From 01 Apr. 2022, To 31 Mar. 2027, Granted

  X染色体の不活性化;霊長類;カニクイザル;XIST;SmcHD1

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H02539/
• The study of X chromosome inactivation maintenance in primate.

  Grant-in-Aid for Scientific Research (B)

  Basic Section 42040:Laboratory animal science-related

  Kyoto University

  岡本 郁弘

  From 01 Apr. 2022, To 31 Mar. 2027, Granted

  X inactivation;XIST;カニクイザル;X染色体の不活性化;霊長類;SmcHD1

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K23804/

Education

Part-time lecturer

• From 21 Jun. 2013, To 21 Jun. 2013

  分子生物学特論

  前期, 北海道大学, 大学院生命科学院
• From 01 Oct. 2018, To 01 Oct. 2018

  大学院特別セミナー　医学特論

  後期, 富山大学, 医学薬学教育部