Basic Information

Faculty

• 医学部

Academic Degree

• 修士（薬学）(大阪大学)
• 博士（薬学）(大阪大学)

Research History

• From Apr. 2013, To Mar. 2017

  ERATO斎藤全能性エピゲノムプロジェクト, 研究総括補佐（兼任）
• From Oct. 2010, To Mar. 2017

  ERATO斎藤全能性エピゲノムプロジェクト, グループリーダー（兼任）
• From Apr. 2019, To Present

  京都大学大学院医学研究科 機能微細形態学, 准教授
• From Apr. 2010, To Mar. 2019

  京都大学大学院医学研究科 機能微細形態学, 助教
• From Oct. 2006, To Mar. 2010

  理化学研究所 神戸研究所, 研究員
• From Oct. 2003, To Sep. 2006

  理化学研究所 神戸研究所, 基礎科学特別研究員
• From Apr. 2003, To Sep. 2003

  理化学研究所 神戸研究所, 研究員
• From Apr. 2001, To Mar. 2003

  日本学術振興会特別研究員

ID,URL

• J-Global ID

  201401054503050634

• Researcher number

  50391892

researchmap URL

• https://researchmap.jp/7000009078

Research

Research Areas

• Life sciences, Developmental biology

Papers

• Generation of functional oocytes from male mice in vitro.

  Kenta Murakami; Nobuhiko Hamazaki; Norio Hamada; Go Nagamatsu; Ikuhiro Okamoto; Hiroshi Ohta; Yoshiaki Nosaka; Yukiko Ishikura; Tomoya S Kitajima; Yuichiro Semba; Yuya Kunisaki; Fumio Arai; Koichi Akashi; Mitinori Saitou; Kiyoko Kato; Katsuhiko Hayashi

  Nature, 15 Mar. 2023

  
• Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells.

  Yukiko Ishikura; Hiroshi Ohta; Masahiro Nagano; Mitinori Saitou

  STAR protocols, 16 Sep. 2022

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• Ex vivo reconstitution of fetal oocyte development in humans and cynomolgus monkeys

  Ken Mizuta; Yoshitaka Katou; Baku Nakakita; Aoi Kishine; Yoshiaki Nosaka; Saki Saito; Chizuru Iwatani; Hideaki Tsuchiya; Ikuo Kawamoto; Masataka Nakaya; Tomoyuki Tsukiyama; Masahiro Nagano; Yoji Kojima; Tomonori Nakamura; Yukihiro Yabuta; Akihito Horie; Masaki Mandai; Hiroshi Ohta; Mitinori Saitou

  The EMBO Journal, Aug. 2022

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• Nucleome programming is required for the foundation of totipotency in mammalian germline development

  Masahiro Nagano; Bo Hu; Shihori Yokobayashi; Akitoshi Yamamura; Fumiya Umemura; Mariel Coradin; Hiroshi Ohta; Yukihiro Yabuta; Yukiko Ishikura; Ikuhiro Okamoto; Hiroki Ikeda; Naofumi Kawahira; Yoshiaki Nosaka; Sakura Shimizu; Yoji Kojima; Ken Mizuta; Tomoko Kasahara; Yusuke Imoto; Killian Meehan; Roman Stocsits; Gordana Wutz; Yasuaki Hiraoka; Yasuhiro Murakawa; Takuya Yamamoto; Kikue Tachibana; Jan‐Michel Peters; Leonid A Mirny; Benjamin A Garcia; Jacek Majewski; Mitinori Saitou

  The EMBO Journal, 04 Jul. 2022

  
• In vitro reconstitution of the whole male germ-cell development from mouse pluripotent stem cells.

  Yukiko Ishikura; Hiroshi Ohta; Takuya Sato; Yusuke Murase; Yukihiro Yabuta; Yoji Kojima; Chika Yamashiro; Tomonori Nakamura; Takuya Yamamoto; Takehiko Ogawa; Mitinori Saitou

  Cell stem cell, 01 Sep. 2021

  
• The embryonic ontogeny of the gonadal somatic cells in mice and monkeys.

  Kotaro Sasaki; Akiko Oguchi; Keren Cheng; Yasuhiro Murakawa; Ikuhiro Okamoto; Hiroshi Ohta; Yukihiro Yabuta; Chizuru Iwatani; Hideaki Tsuchiya; Takuya Yamamoto; Yasunari Seita; Mitinori Saitou

  Cell reports, 04 May 2021

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• Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro†.

  Hiroshi Ohta; Yukihiro Yabuta; Kazuki Kurimoto; Tomonori Nakamura; Yusuke Murase; Takuya Yamamoto; Mitinori Saitou

  Biology of reproduction, 11 Feb. 2021

  
• Long-term expansion with germline potential of human primordial germ cell-like cells in vitro.

  Yusuke Murase; Yukihiro Yabuta; Hiroshi Ohta; Chika Yamashiro; Tomonori Nakamura; Takuya Yamamoto; Mitinori Saitou

  The EMBO journal, 02 Nov. 2020

  
• ZGLP1 is a determinant for the oogenic fate in mice.

  So I Nagaoka; Fumio Nakaki; Hidetaka Miyauchi; Yoshiaki Nosaka; Hiroshi Ohta; Yukihiro Yabuta; Kazuki Kurimoto; Katsuhiko Hayashi; Tomonori Nakamura; Takuya Yamamoto; Mitinori Saitou

  Science (New York, N.Y.), 13 Feb. 2020, Peer-reviewed

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• Induction of the Germ-Cell Fate from Pluripotent Stem Cells in Cynomolgus Monkeys.

  Sakai Y; Nakamura T; Okamoto I; Gyobu-Motani S; Ohta H; Yabuta Y; Tsukiyama T; Iwatani C; Tsuchiya H; Ema M; Morizane A; Takahashi J; Yamamoto T; Saitou M

  Biology of reproduction, Nov. 2019, Peer-reviewed

  
• Establishment of trophoblast stem cell lines from somatic cell nuclear-transferred embryos.

  Oda M; Tanaka S; Yamazaki Y; Ohta H; Iwatani M; Suzuki M; Ohgane J; Hattori N; Yanagimachi R; Wakayama T; Shiota K

  Proceedings of the National Academy of Sciences of the United States of America, Sep. 2009, Peer-reviewed

  
• Long-term preservation of mouse spermatozoa as frozen testicular sections.

  Ohta H; Sakaide Y; Wakayama T

  The Journal of reproduction and development, Aug. 2008, Peer-reviewed

  
• Harmful or not: Trichostatin A treatment of embryos generated by ICSI or ROSI

  Satoshi Kishigami; Hiroshi Ohta; Eiji Mizutani; Sayaka Wakayama; Hong-Thuy Bui; Nguyen Van Thuan; Takafusa Hikichi; Rinako Suetsugu; Teruhiko Wakayama

  CENTRAL EUROPEAN JOURNAL OF BIOLOGY, Sep. 2006, Peer-reviewed

  
• Differentiation potential of parthenogenetic ES cells is improved by nuclear transfer.

  Takafusa Hikichi; Takashi Kohda; Hiroshi Ohta; Sayaka Wakayama; Fumitoshi Ishino; Teruhiko Wakayama

  BIOLOGY OF REPRODUCTION, 2006, Peer-reviewed

  
• mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility.

  Satoko Sakamoto; Dean Thumkeo; Hiroshi Ohta; Zhen Zhang; Shuangru Huang; Pakorn Kanchanawong; Takayoshi Fuu; Sadanori Watanabe; Kentaro Shimada; Yoshitaka Fujihara; Shosei Yoshida; Masahito Ikawa; Naoki Watanabe; Mitinori Saitou; Shuh Narumiya

  PLoS biology, Sep. 2018, Peer-reviewed

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• Induction of fetal primary oocytes and the meiotic prophase from mouse pluripotent stem cells

  Hidetaka Miyauchi; Hiroshi Ohta; Mitinori Saitou

  Methods in Cell Biology, 01 Jan. 2018, Peer-reviewed

  
• Principles for the regulation of multiple developmental pathways by a versatile transcriptional factor, BLIMP1

  Tadahiro Mitani; Yukihiro Yabuta; Hiroshi Ohta; Tomonori Nakamura; Chika Yamashiro; Takuya Yamamoto; Mitinori Saitou; Kazuki Kurimoto

  NUCLEIC ACIDS RESEARCH, Dec. 2017, Peer-reviewed

  
• Bone morphogenetic protein and retinoic acid synergistically specify female germ-cell fate in mice.

  Hidetaka Miyauchi; Hiroshi Ohta; So Nagaoka; Fumio Nakaki; Kotaro Sasaki; Katsuhiko Hayashi; Yukihiro Yabuta; Tomonori Nakamura; Takuya Yamamoto; Mitinori Saitou

  The EMBO journal, 02 Nov. 2017, Peer-reviewed

  
• Fertile offspring from sterile sex chromosome trisomic mice

  Takayuki Hirota; Hiroshi Ohta; Benjamin E. Powell; Shantha K. Mahadevaiah; Obah A. Ojarikre; Mitinori Saitou; James M. A. Turner

  SCIENCE, Sep. 2017, Peer-reviewed

  
• Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis

  Arata Honda; Narantsog Choijookhuu; Haruna Izu; Yoshihiro Kawano; Mizuho Inokuchi; Kimiko Honsho; Ah-Reum Lee; Hiroki Nabekura; Hiroshi Ohta; Tomoyuki Tsukiyama; Yasuhide Ohinata; Asato Kuroiwa; Yoshitaka Hishikawa; Mitinori Saitou; Takamichi Jogahara; Chihiro Koshimoto

  SCIENCE ADVANCES, May 2017, Peer-reviewed

  
• In vitro expansion of mouse primordial germ cell-like cells recapitulates an epigenetic blank slate.

  Ohta H; Kurimoto K; Okamoto I; Nakamura T; Yabuta Y; Miyauchi H; Yamamoto T; Okuno Y; Hagiwara M; Shirane K; Sasaki H; Saitou M

  The EMBO journal, May 2017, Peer-reviewed

  
• In Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells.

  Ishikura Y; Yabuta Y; Ohta H; Hayashi K; Nakamura T; Okamoto I; Yamamoto T; Kurimoto K; Shirane K; Sasaki H; Saitou M

  Cell reports, Dec. 2016, Peer-reviewed

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• Persistent Requirement and Alteration of the Key Targets of PRDM1 During Primordial Germ Cell Development in Mice.

  Chika Yamashiro; Takayuki Hirota; Kazuki Kurimoto; Tomonori Nakamura; Yukihiro Yabuta; So I Nagaoka; Hiroshi Ohta; Takuya Yamamoto; Mitinori Saitou

  Biology of reproduction, Jan. 2016, Peer-reviewed

  
• Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells.

  Sasaki K; Yokobayashi S; Nakamura T; Okamoto I; Yabuta Y; Kurimoto K; Ohta H; Moritoki Y; Iwatani C; Tsuchiya H; Nakamura S; Sekiguchi K; Sakuma T; Yamamoto T; Mori T; Woltjen K; Nakagawa M; Yamamoto T; Takahashi K; Yamanaka S

  Cell stem cell, Aug. 2015, Peer-reviewed

  
• Quantitative Dynamics of Chromatin Remodeling during Germ Cell Specification from Mouse Embryonic Stem Cells

  Kazuki Kurimoto; Yukihiro Yabuta; Katsuhiko Hayashi; Hiroshi Ohta; Hiroshi Kiyonari; Tadahiro Mitani; Yoshinobu Moritoki; Kenjiro Kohri; Hiroshi Kimura; Takuya Yamamoto; Yuki Katou; Katsuhiko Shirahige; Mitinori Saitou

  CELL STEM CELL, May 2015, Peer-reviewed

  
• Induction of Primordial Germ Cell-Like Cells From Mouse Embryonic Stem Cells by ERK Signal Inhibition

  Tohru Kimura; Yoshiaki Kaga; Hiroshi Ohta; Mika Odamoto; Yoichi Sekita; Kunpeng Li; Noriko Yamano; Keita Fujikawa; Ayako Isotani; Norihiko Sasaki; Masashi Toyoda; Katsuhiko Hayashi; Masaru Okabe; Takashi Shinohara; Mitinori Saitou; Toru Nakano

  STEM CELLS, Oct. 2014, Peer-reviewed

  
• A mesodermal factor, T, specifies mouse germ cell fate by directly activating germline determinants.

  Shinya Aramaki; Katsuhiko Hayashi; Kazuki Kurimoto; Hiroshi Ohta; Yukihiro Yabuta; Hiroko Iwanari; Yasuhiro Mochizuki; Takao Hamakubo; Yuki Kato; Katsuhiko Shirahige; Mitinori Saitou

  Developmental cell, 09 Dec. 2013, Peer-reviewed

  
• Context-Dependent Wiring of Sox2 Regulatory Networks for Self-Renewal of Embryonic and Trophoblast Stem Cells

  Kenjiro Adachi; Itoshi Nikaido; Hiroshi Ohta; Satoshi Ohtsuka; Hiroki Ura; Mitsutaka Kadota; Teruhiko Wakayama; Hiroki R. Ueda; Hitoshi Niwa

  MOLECULAR CELL, Nov. 2013, Peer-reviewed

  
• PRDM14 ensures naive pluripotency through dual regulation of signaling and epigenetic pathways in mouse embryonic stem cells.

  Masashi Yamaji; Jun Ueda; Katsuhiko Hayashi; Hiroshi Ohta; Yukihiro Yabuta; Kazuki Kurimoto; Ryuichiro Nakato; Yasuhiro Yamada; Katsuhiko Shirahige; Mitinori Saitou

  Cell stem cell, 07 Mar. 2013, Peer-reviewed

  
• Induction of mouse germ-cell fate by transcription factors in vitro

  Fumio Nakaki; Katsuhiko Hayashi; Hiroshi Ohta; Kazuki Kurimoto; Yukihiro Yabuta; Mitinori Saitou

  Nature, 2013, Peer-reviewed

  
• Offspring from Oocytes Derived from in Vitro Primordial Germ Cell-like Cells in Mice

  Katsuhiko Hayashi; Sugako Ogushi; Kazuki Kurimoto; So Shimamoto; Hiroshi Ohta; Mitinori Saitou

  SCIENCE, Nov. 2012, Peer-reviewed

  
• Autonomic neurocristopathy-associated mutations in PHOX2B dysregulate Sox10 expression

  Mayumi Nagashimada; Hiroshi Ohta; Chong Li; Kazuki Nakao; Toshihiro Uesaka; Jean-Francois Brunet; Jeanne Amiel; Delphine Trochet; Teruhiko Wakayama; Hideki Enomoto

  JOURNAL OF CLINICAL INVESTIGATION, Sep. 2012, Peer-reviewed

  
• Drug-Inducible Gene Recombination by the Dppa3-MER Cre MER Transgene in the Developmental Cycle of the Germ Cell Lineage in Mice

  Takayuki Hirota; Hiroshi Ohta; Mayo Shigeta; Hitoshi Niwa; Mitinori Saitou

  BIOLOGY OF REPRODUCTION, Aug. 2011, Peer-reviewed

  
• Reconstitution of the Mouse Germ Cell Specification Pathway in Culture by Pluripotent Stem Cells

  Katsuhiko Hayashi; Hiroshi Ohta; Kazuki Kurimoto; Shinya Aramaki; Mitinori Saitou

  CELL, Aug. 2011, Peer-reviewed

  
• TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice

  Yukihiro Yabuta; Hiroshi Ohta; Takaya Abe; Kazuki Kurimoto; Shinichiro Chuma; Mitinori Saitou

  JOURNAL OF CELL BIOLOGY, Mar. 2011, Peer-reviewed

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• Functional full-term placentas formed from parthenogenetic embryos using serial nuclear transfer

  Takafusa Hikichi; Hiroshi Ohta; Sayaka Wakayama; Teruhiko Wakayama

  DEVELOPMENT, Sep. 2010, Peer-reviewed

  
• DNA Methylation Is Dispensable for the Growth and Survival of the Extraembryonic Lineages

  Morito Sakaue; Hiroshi Ohta; Yuichi Kumaki; Masaaki Oda; Yuko Sakaide; Chisa Matsuoka; Akiko Yamagiwa; Hitoshi Niwa; Teruhiko Wakayama; Masaki Okano

  CURRENT BIOLOGY, Aug. 2010, Peer-reviewed

  
• Male Germline and Embryonic Stem Cell Lines from NOD Mice: Efficient Derivation of GS Cells from a Nonpermissive Strain for ES Cell Derivation

  Hiroshi Ohta; Yasuhide Ohinata; Masahito Ikawa; Yuka Morioka; Yuko Sakaide; Mitinori Saitou; Osami Kanagawa; Teruhiko Wakayama

  BIOLOGY OF REPRODUCTION, Dec. 2009, Peer-reviewed

  
• A Signaling Principle for the Specification of the Germ Cell Lineage in Mice

  Yasuhide Ohinata; Hiroshi Ohta; Mayo Shigeta; Kaori Yamanaka; Teruhiko Wakayama; Mitinori Saitou

  CELL, May 2009, Peer-reviewed

  
• Age- and substrain-dependent sperm abnormalities in BALB/c mice and functional assessment of abnormal sperm by ICSI

  Hiroshi Ohta; Yuko Sakaide; Teruhiko Wakayama

  HUMAN REPRODUCTION, Apr. 2009, Peer-reviewed

  
• Functional Analysis of Male Mouse Haploid Germ Cells of Various Differentiation Stages: Early and Late Round Spermatids Are Functionally Equivalent in Producing Progeny

  Hiroshi Ohta; Yuko Sakaide; Teruhiko Wakayama

  BIOLOGY OF REPRODUCTION, Mar. 2009, Peer-reviewed

  
• Generation of mice derived from embryonic stem cells using blastocysts of different developmental ages

  Hiroshi Ohta; Yuko Sakaide; Teruhiko Wakayama

  REPRODUCTION, Nov. 2008, Peer-reviewed

  
• Production of healthy cloned mice from bodies frozen at-20 degrees C for 16 years

  Sayaka Wakayama; Hiroshi Ohta; Takafusa Hikichi; Eiji Mizutani; Takamasa Iwaki; Osami Kanagawa; Teruhiko Wakayama

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Nov. 2008, Peer-reviewed

  
• Increasing the cell number of host tetraploid embryos can improve the production of mice derived from embryonic stem cells

  Hiroshi Ohta; Yuko Sakaide; Kazuo Yamagata; Teruhiko Wakayama

  BIOLOGY OF REPRODUCTION, Sep. 2008, Peer-reviewed

  
• Effect of human leukemia cells in testicular tissues grafted into immunodeficient mice

  Kazutoshi Fujita; Akira Tsujimura; Toshiaki Hirai; Hiroshi Ohta; Yasuhiro Matsuoka; Yasushi Miyagawa; Tetsuya Takao; Shingo Takada; Norio Nonomura; Akihiko Okuyama

  INTERNATIONAL JOURNAL OF UROLOGY, Aug. 2008, Peer-reviewed

  
• Effect of volume of oocyte cytoplasm on embryo development after parthenogenetic activation, intracytoplasmic sperm injection, or somatic cell nuclear transfer

  Wakayama Sayaka; Kishigami Satoshi; Nguyen Van Thuan; Ohta Hiroshi; Hikichi Takafusa; Mizutani Eiji; Bui Hong Thuy; Miyake Masashi; Wakayama Teruhiko

  ZYGOTE, Aug. 2008, Peer-reviewed

  
• The birth of mice from testicular spermatozoa retrieved from frozen testicular sections

  Hiroshi Ohta; Yuko Sakaide; Teruhiko Wakayama

  BIOLOGY OF REPRODUCTION, May 2008, Peer-reviewed

  
• Therapeutic cloning in individual parkinsonian mice

  Viviane Tabar; Mark Tomishima; Georgia Panagiotakos; Sayaka Wakayama; Jayanthi Menon; Bill Chan; Eiji Mizutani; George Al-Shamy; Hiroshi Ohta; Teruhiko Wakayama; Lorenz Studer

  NATURE MEDICINE, Apr. 2008, Peer-reviewed

  
• Generation of progeny via ICSI following enrichment of elongated spermatids from mouse testis by flow-cytometric cell sorting

  Hiroshi Ohta; Yuko Sakaide; Teruhiko Wakayama

  HUMAN REPRODUCTION, Jun. 2007, Peer-reviewed

  
• The 193-base pair Gsg2 (haspin) promoter region regulates germ cell-specific expression bidirectionally and synchronously

  Keizo Tokuhiro; Yasushi Miyagawa; Shuichi Yamada; Mika Hirose; Hiroshi Ohta; Yoshitake Nishimune; Hiromitsu Tanaka

  BIOLOGY OF REPRODUCTION, Mar. 2007, Peer-reviewed

  
• Successful mouse cloning of an outbred strain by trichostatin A treatment after somatic nuclear transfer

  Satoshi Kishigami; Hong-Thuy Bui; Sayaka Wakayama; Kenzo Tokunaga; Nguyen Van Thuan; Takafusa Hikichi; Eiji Mizutani; Hiroshi Ohta; Rinako Suetsugu; Tetsutaro Sata; Teruhiko Wakayama

  JOURNAL OF REPRODUCTION AND DEVELOPMENT, Feb. 2007, Peer-reviewed

  
• Establishment of mouse embryonic stem cell lines from somatic cell nuclei by nuclear transfer into aged, fertilization-failure mouse oocytes

  Sayaka Wakayama; Rinako Suetsugu; Nguyen Van Thuan; Hiroshi Ohta; Satoshi Kishigami; Teruhiko Wakayama

  CURRENT BIOLOGY, Feb. 2007, Peer-reviewed

  
• Regulation of chromatin and chromosome morphology by histone H3 modifications in pig oocytes

  Hong-Thuy Bui; Nguyen Van Thuan; Satoshi Kishigami; Sayaka Wakayama; Takafusa Hikichi; Hiroshi Ohta; Eiji Mizutani; Emi Yamaoka; Teruhiko Wakayama; Takashi Miyano

  REPRODUCTION, Feb. 2007, Peer-reviewed

  
• Differentiation potential of parthenogenetic embryonic stem cells is improved by nuclear transfer

  Takafusa Hikichi; Sayaka Wakayama; Eiji Mizutani; Yasuhiro Takashima; Satoshi Kishigami; Nguyen Van Thuan; Hiroshi Ohta; Hong Thuy Bui; Shin-Ichi Nishikawa; Teruhiko Wakayama

  STEM CELLS, Jan. 2007, Peer-reviewed

  
• Developmental ability of cloned embryos from neural stem cells

  Eiji Mizutani; Hiroshi Ohta; Satoshi Kishigami; Nguyen Van Thuan; Takafusa Hikichi; Sayaka Wakayama; Mitsuko Kosaka; Eimei Sato; Teruhiko Wakayama

  REPRODUCTION, Dec. 2006, Peer-reviewed

  
• Carboxypeptidase E in the mouse placenta

  Umashankar Singh; Yang Yu; Elena Kalinina; Toshihiro Konno; Tong Sun; Hiroshi Ohta; Teruhiko Wakayama; Michael J. Soares; Myriam Hemberger; Reinald H. Fundele

  DIFFERENTIATION, Dec. 2006, Peer-reviewed

  
• Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts

  Sayaka Wakayama; Martin L. Jakt; Masako Suzuki; Ryoko Araki; Takafusa Hikichi; Satoshi Kishigami; Hiroshi Ohta; Nguyen Van Thuan; Eiji Mizutani; Yuko Sakaide; Sho Senda; Satoshi Tanaka; Mitsuhiro Okada; Masashi Miyake; Masumi Abe; Shin-Ichi Nishikawa; Kunio Shiota; Teruhiko Wakayama

  STEM CELLS, Sep. 2006, Peer-reviewed

  
• Injection of somatic cell cytoplasm into oocytes before intracytoplasmic sperm injection impairs full-term development and increases placental weight in mice

  N Van Thuan; S Wakayama; S Kishigami; H Ohta; T Hikichi; E Mizutani; HT Bui; T Wakayama

  BIOLOGY OF REPRODUCTION, May 2006, Peer-reviewed

  
• Normal specification of the extraembryonic lineage after somatic nuclear transfer

  S Kishigami; T Hikichi; N Van Thuan; H Ohta; S Wakayama; HT Bui; E Mizutani; T Wakayama

  FEBS LETTERS, Mar. 2006, Peer-reviewed

  
• Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer

  S Kishigami; E Mizutani; H Ohta; T Hikichi; N Van Thuan; S Wakayama; HT Bui; T Wakayama

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Feb. 2006, Peer-reviewed

  
• Retinal stem/progenitor properties of iris pigment epithelial cells

  GW Sun; M Asami; H Ohta; J Kosaka; M Kosaka

  DEVELOPMENTAL BIOLOGY, Jan. 2006, Peer-reviewed

  
• Epigenetic abnormalities of the mouse paternal zygotic genome associated with microinsemination of round spermatids

  S Kishigami; N Van Thuan; T Hikichi; H Ohta; S Wakayama; E Mizutani; T Wakayama

  DEVELOPMENTAL BIOLOGY, Jan. 2006, Peer-reviewed

  
• Production of cloned mice by somatic cell nuclear transfer

  Satoshi Kishigami; Sayaka Wakayama; Nguyen Van Thuan; Hiroshi Ohta; Eiji Mizutani; Takafusa Hikichi; Hong-Thuy Bui; Sebastian Balbach; Atsuo Ogura; Michele Boiani; Teruhiko Wakayama

  NATURE PROTOCOLS, 2006, Peer-reviewed

  
• Mice cloned by nuclear transfer from somatic and ntES cells derived from the same individuals

  S Wakayama; E Mizutani; S Kishigami; N Van Thuan; H Ohta; T Hikichi; HT Bui; M Miyake; T Wakayama

  JOURNAL OF REPRODUCTION AND DEVELOPMENT, Dec. 2005, Peer-reviewed

  
• Expression and functional analysis of genes deregulated in mouse placental overgrowth models: Car2 and Ncam1

  U Singh; T Sun; W Shi; R Schulz; UA Nuber; A Varanou; MC Hemberger; RW Elliott; H Ohta; T Wakayama; R Fundele

  DEVELOPMENTAL DYNAMICS, Dec. 2005, Peer-reviewed

  
• Generation of normal progeny by intracytoplasmic sperm injection following grafting of testicular tissue from cloned mice that died postnatally

  H Ohta; T Wakayama

  BIOLOGY OF REPRODUCTION, Sep. 2005, Peer-reviewed

  
• Generation of progeny from embryonic stem cells by microinsemination of male germ cells from chimeric mice

  E Mizutani; H Ohta; S Kishigami; N Van Thuan; T Hikichi; S Wakayama; E Sato; T Wakayama

  GENESIS, Sep. 2005, Peer-reviewed

  
• Transplantation of spermatogonial stem cells isolated from leukemic mice restores fertility without inducing leukemia

  K Fujita; H Ohta; A Tsujimura; T Takao; Y Miyagawa; S Takada; K Matsumiya; T Wakayama; A Okuyama

  JOURNAL OF CLINICAL INVESTIGATION, Jul. 2005, Peer-reviewed

  
• Establishment of male and female nuclear transfer embryonic stem cell lines from different mouse strains and tissues

  S Wakayama; H Ohta; S Kishigami; N Van Thuan; T Hikichi; E Mizutani; M Miyake; T Wakayama

  BIOLOGY OF REPRODUCTION, Apr. 2005, Peer-reviewed

  
• Round spermatids stained with MitoTracker can be used to produce offspring more simply

  T Hikichi; S Kishigami; N Van Thuan; H Ohta; E Mizutani; S Wakayama; T Wakayama

  ZYGOTE, Feb. 2005, Peer-reviewed

  
• Propagation of an infertile hermaphrodite mouse lacking germ cells by using nuclear transfer and embryonic stem cell technology

  S Wakayama; S Kishigami; N Van Thuan; H Ohta; T Hikichi; E Mizutani; R Yanagimachi; T Wakayama

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Jan. 2005, Peer-reviewed

  
• cAMP-responsive element in TATA-less core promoter is essential for haploid-specific gene expression in mouse testis

  P Somboonthum; H Ohta; S Yamada; M Onishi; A Ike; Y Nishimune; M Nozaki

  NUCLEIC ACIDS RESEARCH, 2005, Peer-reviewed

  
• Production of offspring from one-day-old oocytes stored at room temperature

  S Wakayama; NV Thuan; S Kishigami; H Ohta; E Mizutani; T Hikichi; M Miyake; T Wakayama

  JOURNAL OF REPRODUCTION AND DEVELOPMENT, Dec. 2004, Peer-reviewed

  
• Full-term development of offspring using round spermatids produced ectopically from fetal male germ cells

  H Ohta; T Wakayama

  JOURNAL OF REPRODUCTION AND DEVELOPMENT, Aug. 2004, Peer-reviewed

  
• Commitment of fetal male germ cells to spermatogonial stem cells during mouse embryonic development

  H Ohta; T Wakayama; Y Nishimune

  BIOLOGY OF REPRODUCTION, May 2004, Peer-reviewed

  
• Rosbin: A novel homeobox-like protein gene expressed exclusively in round spermatids

  T Takahashi; H Tanaka; N Iguchi; K Kitamura; YL Chen; M Maekawa; H Nishimura; H Ohta; Y Miyagawa; K Matsumiya; A Okuyama; Y Nishimune

  BIOLOGY OF REPRODUCTION, May 2004, Peer-reviewed

  
• Transient expression analysis of the mouse ornithine decarboxylase antizyme haploid-specific promoter using in vivo electroporation

  A Ike; H Ohta; M Onishi; N Iguchi; Y Nishimune; M Nozaki

  FEBS LETTERS, Feb. 2004, Peer-reviewed

  
• Proliferation and differentiation of spermatogonial stem cells in the W/W-v mutant mouse testis

  H Ohta; A Tohda; Y Nishimune

  BIOLOGY OF REPRODUCTION, Dec. 2003, Peer-reviewed

  
• Methylation of CpG dinucleotides in the open reading frame of a testicular germ cell-specific intronless gene, Tact1/Actl7b, represses its expression in somatic cells

  M Hisano; H Ohta; Y Nishimune; M Nozaki

  NUCLEIC ACIDS RESEARCH, Aug. 2003, Peer-reviewed

  
• Functional analysis of the p53 gene in apoptosis induced by heat stress or loss of stem cell factor signaling in mouse male germ cells

  H Ohta; S Aizawa; Y Nishimune

  BIOLOGY OF REPRODUCTION, Jun. 2003, Peer-reviewed

  
• Nectin couples cell-cell adhesion and the actin scaffold at heterotypic testicular junctions

  K Ozaki-Kuroda; H Nakanishi; H Ohta; H Tanaka; H Kurihara; S Mueller; K Irie; W Ikeda; T Sakai; E Wimmer; Y Nishimune; Y Takai

  CURRENT BIOLOGY, Jul. 2002, Peer-reviewed

  
• Development of the stages of the cycle in mouse seminiferous epithelium after transplantation of green fluorescent protein-labeled spermatogonial stem cells

  S Ventela; H Ohta; M Parvinen; Y Nishimune

  BIOLOGY OF REPRODUCTION, May 2002, Peer-reviewed

  
• Homeostatic regulation of germinal stem cell proliferation by the GDNF/FSH pathway

  Y Tadokoro; K Yomogida; H Ohta; A Tohda; Y Nishimune

  MECHANISMS OF DEVELOPMENT, Apr. 2002, Peer-reviewed

  
• Defect in germ cells, not in supporting cells, is the cause of male infertility in the jsd mutant mouse: proliferation of spermatogonial stem cells without differentiation

  H Ohta; K Yomogida; Y Tadokoro; A Tohda; K Dohmae; Y Nishimune

  INTERNATIONAL JOURNAL OF ANDROLOGY, Feb. 2001, Peer-reviewed

  
• Isolation and characterization of a haploid germ cell-specific novel complementary deoxyribonucleic acid; Testis-specific homologue of succinyl CoA: 3-oxo acid CoA transferase

  M Koga; H Tanaka; K Yomogida; M Nozaki; J Tsuchida; H Ohta; Y Nakamura; K Masai; Y Yoshimura; M Yamanaka; N Iguchi; H Nojima; K Matsumiya; A Okuyama; Y Nishimune

  BIOLOGY OF REPRODUCTION, Dec. 2000, Peer-reviewed

  
• Molecular cloning and characterization of phosphatidylcholine transfer protein-like protein gene expressed in murine haploid germ cells

  M Yamanaka; M Koga; H Tanaka; Y Nakamura; H Ohta; K Yomogida; J Tsuchida; N Iguchi; H Nojima; M Nozaki; K Matsumiya; A Okuyama; K Toshimori; Y Nishimune

  BIOLOGY OF REPRODUCTION, Jun. 2000, Peer-reviewed

  
• Regulation of proliferation and differentiation in spermatogonial stem cells: the role of c-kit and its ligand SCF

  H Ohta; K Yomogida; K Dohmae; Y Nishimune

  DEVELOPMENT, May 2000, Peer-reviewed

  
• Real-time observation of transplanted 'green germ cells': Proliferation and differentiation of stem cells

  H Ohta; K Yomogida; S Yamada; M Okabe; Y Nishimune

  DEVELOPMENT GROWTH & DIFFERENTIATION, Apr. 2000, Peer-reviewed

  

Misc.

• 核移植胚への同一ntES細胞注入によるクローン作出効率改善の試み

  水谷英二; 岸上哲士; NGUYEN Van Thuan; 大田浩; 引地貴亮; BUI Hong Thuy; 若山清香; 若山照彦

  J Reprod Dev, 25 Aug. 2006

  
• 極体および初期胚割球からのES細胞樹立の試み

  若山清香; 岸上哲士; 坂出裕子; NGUYEN Van Thuan; 大田浩; 引地貴亮; 水谷英二; BUI Hong Thuy; 若山照彦

  J Reprod Dev, 25 Aug. 2006

  

External funds: Kakenhi

• Establish a culture system for somatic ovarian cells that can support in vitro oogenesis

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 48:Biomedical structure and function and related fields

  Kyoto University

  Hiroshi Ohta

  From 30 Jul. 2020, To 31 Mar. 2023, Project Closed

  卵子形成;再構成卵巣;雌性生殖巣体細胞;前顆粒膜細胞;細胞培養;PGCLC;マウス;生殖巣;体細胞;培養;支持細胞;始原生殖細胞

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K21500/
• Elucidation of the gene expression cascade responsible for male sexuality and acquisition of fertility

  Grant-in-Aid for Scientific Research (A)

  Medium-sized Section 44:Biology at cellular to organismal levels, and related fields

  Jichi Medical University

  本多 新

  From 01 Apr. 2020, To 31 Mar. 2025, Granted

  性決定;絶滅危惧種;iPS細胞;生殖巣;生殖細胞;アマミトゲネズミ;胚盤胞補完法;マウス;ラット;生殖細胞体外再構築

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20H00469/
• Establishment of culture system for inducing oocytes from primordial germ cell-like cells

  Grant-in-Aid for Scientific Research (B)

  Basic Section 52050:Embryonic medicine and pediatrics-related

  Kyoto University

  Hiroshi Ohta

  From 01 Apr. 2019, To 31 Mar. 2023, Project Closed

  卵原細胞;卵母細胞;卵子形成;再構成卵巣;始原生殖細胞;試験管内誘導;ヒト;多能性幹細胞;始原生殖細胞様細胞;試験管内;胎生期生殖巣;iPS細胞;in vitro;細胞分化

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H03618/
• Establishment of efficient induction system for primordial germ cells from ES cells in vitro

  Grant-in-Aid for Challenging Exploratory Research

  Kyoto University

  Hiroshi Ohta

  From 01 Apr. 2015, To 31 Mar. 2017, Project Closed

  始原生殖細胞;ES細胞;EpiLC;分化誘導;増殖;分化

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K14365/
• Analysis of proliferation and differentiation of primordial germ cell-like cells derived from ES cells

  Grant-in-Aid for Young Scientists (A)

  Kyoto University

  Hiroshi Ohta

  From 01 Apr. 2015, To 31 Mar. 2019, Project Closed

  始原生殖細胞;ES細胞;配偶子形成;増殖;分化;細胞培養;培養;細胞増殖;細胞分化;試験管内

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15H05636/
• Establishment of novel technique using primordial germ cells induced from embryonic stem cells

  Grant-in-Aid for Young Scientists (A)

  Kyoto University

  Hiroshi OHTA

  From 01 Apr. 2012, To 31 Mar. 2015, Project Closed

  始原生殖細胞;ES細胞;小分子化合物;増殖・分化;精子形成;卵子形成;分化誘導;培養;chemical libray;high-throughput screening

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24680045/
• ヒト卵原細胞から卵子への試験管内誘導系の確立

  Grant-in-Aid for Scientific Research (B)

  Basic Section 56040:Obstetrics and gynecology-related

  Kyoto University

  大田 浩

  From 01 Apr. 2023, To 31 Mar. 2027, Granted

  卵巣;卵胞;卵母細胞;培養;ヒト

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23H03042/
• Establishment of an in vitro induction system from human oogonia to oocytes.

  Grant-in-Aid for Scientific Research (B)

  Basic Section 56040:Obstetrics and gynecology-related

  Kyoto University

  大田 浩

  From 01 Apr. 2023, To 31 Mar. 2027, Granted

  卵原細胞;卵子;卵胞;ヒト;培養;原始卵胞;卵子形成;卵巣;卵母細胞

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K27733/

Administration

Faculty management (title, position)

• From 01 Apr. 2023, To 31 Mar. 2024

  医学部構内実験排水系管理委員会委員
• From 01 Apr. 2013, To 31 Mar. 2019

  医学図書館図書連絡委員
• From 01 Apr. 2015, To 31 Mar. 2019

  動物実験施設運営委員会
• From 01 Apr. 2021, To 31 Mar. 2022

  医学部構内実験排水系管理委員会委員