基本情報

学部兼担

• 医学部

学位

• 修士（薬学）(大阪大学)
• 博士（薬学）(大阪大学)

経歴

• 自 2013年04月, 至 2017年03月

  ERATO斎藤全能性エピゲノムプロジェクト, 研究総括補佐（兼任）
• 自 2010年10月, 至 2017年03月

  ERATO斎藤全能性エピゲノムプロジェクト, グループリーダー（兼任）
• 自 2019年04月, 至 現在

  京都大学大学院医学研究科 機能微細形態学, 准教授
• 自 2010年04月, 至 2019年03月

  京都大学大学院医学研究科 機能微細形態学, 助教
• 自 2006年10月, 至 2010年03月

  理化学研究所 神戸研究所, 研究員
• 自 2003年10月, 至 2006年09月

  理化学研究所 神戸研究所, 基礎科学特別研究員
• 自 2003年04月, 至 2003年09月

  理化学研究所 神戸研究所, 研究員
• 自 2001年04月, 至 2003年03月

  日本学術振興会特別研究員

ID,URL

• J-Global ID

  201401054503050634

• 研究者番号

  50391892

researchmap URL

• https://researchmap.jp/7000009078

研究

研究分野

• ライフサイエンス, 発生生物学

論文

• Generation of functional oocytes from male mice in vitro.

  Kenta Murakami; Nobuhiko Hamazaki; Norio Hamada; Go Nagamatsu; Ikuhiro Okamoto; Hiroshi Ohta; Yoshiaki Nosaka; Yukiko Ishikura; Tomoya S Kitajima; Yuichiro Semba; Yuya Kunisaki; Fumio Arai; Koichi Akashi; Mitinori Saitou; Kiyoko Kato; Katsuhiko Hayashi

  Nature, 2023年03月15日

  
• Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells.

  Yukiko Ishikura; Hiroshi Ohta; Masahiro Nagano; Mitinori Saitou

  STAR protocols, 2022年09月16日

  この論文にアクセスする：

  
• Ex vivo reconstitution of fetal oocyte development in humans and cynomolgus monkeys

  Ken Mizuta; Yoshitaka Katou; Baku Nakakita; Aoi Kishine; Yoshiaki Nosaka; Saki Saito; Chizuru Iwatani; Hideaki Tsuchiya; Ikuo Kawamoto; Masataka Nakaya; Tomoyuki Tsukiyama; Masahiro Nagano; Yoji Kojima; Tomonori Nakamura; Yukihiro Yabuta; Akihito Horie; Masaki Mandai; Hiroshi Ohta; Mitinori Saitou

  The EMBO Journal, 2022年08月

  この論文にアクセスする：

  
• Nucleome programming is required for the foundation of totipotency in mammalian germline development

  Masahiro Nagano; Bo Hu; Shihori Yokobayashi; Akitoshi Yamamura; Fumiya Umemura; Mariel Coradin; Hiroshi Ohta; Yukihiro Yabuta; Yukiko Ishikura; Ikuhiro Okamoto; Hiroki Ikeda; Naofumi Kawahira; Yoshiaki Nosaka; Sakura Shimizu; Yoji Kojima; Ken Mizuta; Tomoko Kasahara; Yusuke Imoto; Killian Meehan; Roman Stocsits; Gordana Wutz; Yasuaki Hiraoka; Yasuhiro Murakawa; Takuya Yamamoto; Kikue Tachibana; Jan‐Michel Peters; Leonid A Mirny; Benjamin A Garcia; Jacek Majewski; Mitinori Saitou

  The EMBO Journal, 2022年07月04日

  
• In vitro reconstitution of the whole male germ-cell development from mouse pluripotent stem cells.

  Yukiko Ishikura; Hiroshi Ohta; Takuya Sato; Yusuke Murase; Yukihiro Yabuta; Yoji Kojima; Chika Yamashiro; Tomonori Nakamura; Takuya Yamamoto; Takehiko Ogawa; Mitinori Saitou

  Cell stem cell, 2021年09月01日

  
• The embryonic ontogeny of the gonadal somatic cells in mice and monkeys.

  Kotaro Sasaki; Akiko Oguchi; Keren Cheng; Yasuhiro Murakawa; Ikuhiro Okamoto; Hiroshi Ohta; Yukihiro Yabuta; Chizuru Iwatani; Hideaki Tsuchiya; Takuya Yamamoto; Yasunari Seita; Mitinori Saitou

  Cell reports, 2021年05月04日

  この論文にアクセスする：

  
• Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro†.

  Hiroshi Ohta; Yukihiro Yabuta; Kazuki Kurimoto; Tomonori Nakamura; Yusuke Murase; Takuya Yamamoto; Mitinori Saitou

  Biology of reproduction, 2021年02月11日

  
• Long-term expansion with germline potential of human primordial germ cell-like cells in vitro.

  Yusuke Murase; Yukihiro Yabuta; Hiroshi Ohta; Chika Yamashiro; Tomonori Nakamura; Takuya Yamamoto; Mitinori Saitou

  The EMBO journal, 2020年11月02日

  
• ZGLP1 is a determinant for the oogenic fate in mice.

  So I Nagaoka; Fumio Nakaki; Hidetaka Miyauchi; Yoshiaki Nosaka; Hiroshi Ohta; Yukihiro Yabuta; Kazuki Kurimoto; Katsuhiko Hayashi; Tomonori Nakamura; Takuya Yamamoto; Mitinori Saitou

  Science (New York, N.Y.), 2020年02月13日, 査読有り

  この論文にアクセスする：

  
• Induction of the Germ-Cell Fate from Pluripotent Stem Cells in Cynomolgus Monkeys.

  Sakai Y; Nakamura T; Okamoto I; Gyobu-Motani S; Ohta H; Yabuta Y; Tsukiyama T; Iwatani C; Tsuchiya H; Ema M; Morizane A; Takahashi J; Yamamoto T; Saitou M

  Biology of reproduction, 2019年11月, 査読有り

  
• Establishment of trophoblast stem cell lines from somatic cell nuclear-transferred embryos.

  Oda M; Tanaka S; Yamazaki Y; Ohta H; Iwatani M; Suzuki M; Ohgane J; Hattori N; Yanagimachi R; Wakayama T; Shiota K

  Proceedings of the National Academy of Sciences of the United States of America, 2009年09月, 査読有り

  
• Long-term preservation of mouse spermatozoa as frozen testicular sections.

  Ohta H; Sakaide Y; Wakayama T

  The Journal of reproduction and development, 2008年08月, 査読有り

  
• Harmful or not: Trichostatin A treatment of embryos generated by ICSI or ROSI

  Satoshi Kishigami; Hiroshi Ohta; Eiji Mizutani; Sayaka Wakayama; Hong-Thuy Bui; Nguyen Van Thuan; Takafusa Hikichi; Rinako Suetsugu; Teruhiko Wakayama

  CENTRAL EUROPEAN JOURNAL OF BIOLOGY, 2006年09月, 査読有り

  
• Differentiation potential of parthenogenetic ES cells is improved by nuclear transfer.

  Takafusa Hikichi; Takashi Kohda; Hiroshi Ohta; Sayaka Wakayama; Fumitoshi Ishino; Teruhiko Wakayama

  BIOLOGY OF REPRODUCTION, 2006年, 査読有り

  
• mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility.

  Satoko Sakamoto; Dean Thumkeo; Hiroshi Ohta; Zhen Zhang; Shuangru Huang; Pakorn Kanchanawong; Takayoshi Fuu; Sadanori Watanabe; Kentaro Shimada; Yoshitaka Fujihara; Shosei Yoshida; Masahito Ikawa; Naoki Watanabe; Mitinori Saitou; Shuh Narumiya

  PLoS biology, 2018年09月, 査読有り

  この論文にアクセスする：

  
• Induction of fetal primary oocytes and the meiotic prophase from mouse pluripotent stem cells

  Hidetaka Miyauchi; Hiroshi Ohta; Mitinori Saitou

  Methods in Cell Biology, 2018年01月01日, 査読有り

  
• Principles for the regulation of multiple developmental pathways by a versatile transcriptional factor, BLIMP1

  Tadahiro Mitani; Yukihiro Yabuta; Hiroshi Ohta; Tomonori Nakamura; Chika Yamashiro; Takuya Yamamoto; Mitinori Saitou; Kazuki Kurimoto

  NUCLEIC ACIDS RESEARCH, 2017年12月, 査読有り

  
• Bone morphogenetic protein and retinoic acid synergistically specify female germ-cell fate in mice.

  Hidetaka Miyauchi; Hiroshi Ohta; So Nagaoka; Fumio Nakaki; Kotaro Sasaki; Katsuhiko Hayashi; Yukihiro Yabuta; Tomonori Nakamura; Takuya Yamamoto; Mitinori Saitou

  The EMBO journal, 2017年11月02日, 査読有り

  
• Fertile offspring from sterile sex chromosome trisomic mice

  Takayuki Hirota; Hiroshi Ohta; Benjamin E. Powell; Shantha K. Mahadevaiah; Obah A. Ojarikre; Mitinori Saitou; James M. A. Turner

  SCIENCE, 2017年09月, 査読有り

  
• Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis

  Arata Honda; Narantsog Choijookhuu; Haruna Izu; Yoshihiro Kawano; Mizuho Inokuchi; Kimiko Honsho; Ah-Reum Lee; Hiroki Nabekura; Hiroshi Ohta; Tomoyuki Tsukiyama; Yasuhide Ohinata; Asato Kuroiwa; Yoshitaka Hishikawa; Mitinori Saitou; Takamichi Jogahara; Chihiro Koshimoto

  SCIENCE ADVANCES, 2017年05月, 査読有り

  
• In vitro expansion of mouse primordial germ cell-like cells recapitulates an epigenetic blank slate.

  Ohta H; Kurimoto K; Okamoto I; Nakamura T; Yabuta Y; Miyauchi H; Yamamoto T; Okuno Y; Hagiwara M; Shirane K; Sasaki H; Saitou M

  The EMBO journal, 2017年05月, 査読有り

  
• In Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells.

  Ishikura Y; Yabuta Y; Ohta H; Hayashi K; Nakamura T; Okamoto I; Yamamoto T; Kurimoto K; Shirane K; Sasaki H; Saitou M

  Cell reports, 2016年12月, 査読有り

  この論文にアクセスする：

  
• Persistent Requirement and Alteration of the Key Targets of PRDM1 During Primordial Germ Cell Development in Mice.

  Chika Yamashiro; Takayuki Hirota; Kazuki Kurimoto; Tomonori Nakamura; Yukihiro Yabuta; So I Nagaoka; Hiroshi Ohta; Takuya Yamamoto; Mitinori Saitou

  Biology of reproduction, 2016年01月, 査読有り

  
• Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells.

  Sasaki K; Yokobayashi S; Nakamura T; Okamoto I; Yabuta Y; Kurimoto K; Ohta H; Moritoki Y; Iwatani C; Tsuchiya H; Nakamura S; Sekiguchi K; Sakuma T; Yamamoto T; Mori T; Woltjen K; Nakagawa M; Yamamoto T; Takahashi K; Yamanaka S

  Cell stem cell, 2015年08月, 査読有り

  
• Quantitative Dynamics of Chromatin Remodeling during Germ Cell Specification from Mouse Embryonic Stem Cells

  Kazuki Kurimoto; Yukihiro Yabuta; Katsuhiko Hayashi; Hiroshi Ohta; Hiroshi Kiyonari; Tadahiro Mitani; Yoshinobu Moritoki; Kenjiro Kohri; Hiroshi Kimura; Takuya Yamamoto; Yuki Katou; Katsuhiko Shirahige; Mitinori Saitou

  CELL STEM CELL, 2015年05月, 査読有り

  
• Induction of Primordial Germ Cell-Like Cells From Mouse Embryonic Stem Cells by ERK Signal Inhibition

  Tohru Kimura; Yoshiaki Kaga; Hiroshi Ohta; Mika Odamoto; Yoichi Sekita; Kunpeng Li; Noriko Yamano; Keita Fujikawa; Ayako Isotani; Norihiko Sasaki; Masashi Toyoda; Katsuhiko Hayashi; Masaru Okabe; Takashi Shinohara; Mitinori Saitou; Toru Nakano

  STEM CELLS, 2014年10月, 査読有り

  
• A mesodermal factor, T, specifies mouse germ cell fate by directly activating germline determinants.

  Shinya Aramaki; Katsuhiko Hayashi; Kazuki Kurimoto; Hiroshi Ohta; Yukihiro Yabuta; Hiroko Iwanari; Yasuhiro Mochizuki; Takao Hamakubo; Yuki Kato; Katsuhiko Shirahige; Mitinori Saitou

  Developmental cell, 2013年12月09日, 査読有り

  
• Context-Dependent Wiring of Sox2 Regulatory Networks for Self-Renewal of Embryonic and Trophoblast Stem Cells

  Kenjiro Adachi; Itoshi Nikaido; Hiroshi Ohta; Satoshi Ohtsuka; Hiroki Ura; Mitsutaka Kadota; Teruhiko Wakayama; Hiroki R. Ueda; Hitoshi Niwa

  MOLECULAR CELL, 2013年11月, 査読有り

  
• PRDM14 ensures naive pluripotency through dual regulation of signaling and epigenetic pathways in mouse embryonic stem cells.

  Masashi Yamaji; Jun Ueda; Katsuhiko Hayashi; Hiroshi Ohta; Yukihiro Yabuta; Kazuki Kurimoto; Ryuichiro Nakato; Yasuhiro Yamada; Katsuhiko Shirahige; Mitinori Saitou

  Cell stem cell, 2013年03月07日, 査読有り

  
• Induction of mouse germ-cell fate by transcription factors in vitro

  Fumio Nakaki; Katsuhiko Hayashi; Hiroshi Ohta; Kazuki Kurimoto; Yukihiro Yabuta; Mitinori Saitou

  Nature, 2013年, 査読有り

  
• Offspring from Oocytes Derived from in Vitro Primordial Germ Cell-like Cells in Mice

  Katsuhiko Hayashi; Sugako Ogushi; Kazuki Kurimoto; So Shimamoto; Hiroshi Ohta; Mitinori Saitou

  SCIENCE, 2012年11月, 査読有り

  
• Autonomic neurocristopathy-associated mutations in PHOX2B dysregulate Sox10 expression

  Mayumi Nagashimada; Hiroshi Ohta; Chong Li; Kazuki Nakao; Toshihiro Uesaka; Jean-Francois Brunet; Jeanne Amiel; Delphine Trochet; Teruhiko Wakayama; Hideki Enomoto

  JOURNAL OF CLINICAL INVESTIGATION, 2012年09月, 査読有り

  
• Drug-Inducible Gene Recombination by the Dppa3-MER Cre MER Transgene in the Developmental Cycle of the Germ Cell Lineage in Mice

  Takayuki Hirota; Hiroshi Ohta; Mayo Shigeta; Hitoshi Niwa; Mitinori Saitou

  BIOLOGY OF REPRODUCTION, 2011年08月, 査読有り

  
• Reconstitution of the Mouse Germ Cell Specification Pathway in Culture by Pluripotent Stem Cells

  Katsuhiko Hayashi; Hiroshi Ohta; Kazuki Kurimoto; Shinya Aramaki; Mitinori Saitou

  CELL, 2011年08月, 査読有り

  
• TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice

  Yukihiro Yabuta; Hiroshi Ohta; Takaya Abe; Kazuki Kurimoto; Shinichiro Chuma; Mitinori Saitou

  JOURNAL OF CELL BIOLOGY, 2011年03月, 査読有り

  この論文にアクセスする：

  
• Functional full-term placentas formed from parthenogenetic embryos using serial nuclear transfer

  Takafusa Hikichi; Hiroshi Ohta; Sayaka Wakayama; Teruhiko Wakayama

  DEVELOPMENT, 2010年09月, 査読有り

  
• DNA Methylation Is Dispensable for the Growth and Survival of the Extraembryonic Lineages

  Morito Sakaue; Hiroshi Ohta; Yuichi Kumaki; Masaaki Oda; Yuko Sakaide; Chisa Matsuoka; Akiko Yamagiwa; Hitoshi Niwa; Teruhiko Wakayama; Masaki Okano

  CURRENT BIOLOGY, 2010年08月, 査読有り

  
• Male Germline and Embryonic Stem Cell Lines from NOD Mice: Efficient Derivation of GS Cells from a Nonpermissive Strain for ES Cell Derivation

  Hiroshi Ohta; Yasuhide Ohinata; Masahito Ikawa; Yuka Morioka; Yuko Sakaide; Mitinori Saitou; Osami Kanagawa; Teruhiko Wakayama

  BIOLOGY OF REPRODUCTION, 2009年12月, 査読有り

  
• A Signaling Principle for the Specification of the Germ Cell Lineage in Mice

  Yasuhide Ohinata; Hiroshi Ohta; Mayo Shigeta; Kaori Yamanaka; Teruhiko Wakayama; Mitinori Saitou

  CELL, 2009年05月, 査読有り

  
• Age- and substrain-dependent sperm abnormalities in BALB/c mice and functional assessment of abnormal sperm by ICSI

  Hiroshi Ohta; Yuko Sakaide; Teruhiko Wakayama

  HUMAN REPRODUCTION, 2009年04月, 査読有り

  
• Functional Analysis of Male Mouse Haploid Germ Cells of Various Differentiation Stages: Early and Late Round Spermatids Are Functionally Equivalent in Producing Progeny

  Hiroshi Ohta; Yuko Sakaide; Teruhiko Wakayama

  BIOLOGY OF REPRODUCTION, 2009年03月, 査読有り

  
• Generation of mice derived from embryonic stem cells using blastocysts of different developmental ages

  Hiroshi Ohta; Yuko Sakaide; Teruhiko Wakayama

  REPRODUCTION, 2008年11月, 査読有り

  
• Production of healthy cloned mice from bodies frozen at-20 degrees C for 16 years

  Sayaka Wakayama; Hiroshi Ohta; Takafusa Hikichi; Eiji Mizutani; Takamasa Iwaki; Osami Kanagawa; Teruhiko Wakayama

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008年11月, 査読有り

  
• Increasing the cell number of host tetraploid embryos can improve the production of mice derived from embryonic stem cells

  Hiroshi Ohta; Yuko Sakaide; Kazuo Yamagata; Teruhiko Wakayama

  BIOLOGY OF REPRODUCTION, 2008年09月, 査読有り

  
• Effect of human leukemia cells in testicular tissues grafted into immunodeficient mice

  Kazutoshi Fujita; Akira Tsujimura; Toshiaki Hirai; Hiroshi Ohta; Yasuhiro Matsuoka; Yasushi Miyagawa; Tetsuya Takao; Shingo Takada; Norio Nonomura; Akihiko Okuyama

  INTERNATIONAL JOURNAL OF UROLOGY, 2008年08月, 査読有り

  
• Effect of volume of oocyte cytoplasm on embryo development after parthenogenetic activation, intracytoplasmic sperm injection, or somatic cell nuclear transfer

  Wakayama Sayaka; Kishigami Satoshi; Nguyen Van Thuan; Ohta Hiroshi; Hikichi Takafusa; Mizutani Eiji; Bui Hong Thuy; Miyake Masashi; Wakayama Teruhiko

  ZYGOTE, 2008年08月, 査読有り

  
• The birth of mice from testicular spermatozoa retrieved from frozen testicular sections

  Hiroshi Ohta; Yuko Sakaide; Teruhiko Wakayama

  BIOLOGY OF REPRODUCTION, 2008年05月, 査読有り

  
• Therapeutic cloning in individual parkinsonian mice

  Viviane Tabar; Mark Tomishima; Georgia Panagiotakos; Sayaka Wakayama; Jayanthi Menon; Bill Chan; Eiji Mizutani; George Al-Shamy; Hiroshi Ohta; Teruhiko Wakayama; Lorenz Studer

  NATURE MEDICINE, 2008年04月, 査読有り

  
• Generation of progeny via ICSI following enrichment of elongated spermatids from mouse testis by flow-cytometric cell sorting

  Hiroshi Ohta; Yuko Sakaide; Teruhiko Wakayama

  HUMAN REPRODUCTION, 2007年06月, 査読有り

  
• The 193-base pair Gsg2 (haspin) promoter region regulates germ cell-specific expression bidirectionally and synchronously

  Keizo Tokuhiro; Yasushi Miyagawa; Shuichi Yamada; Mika Hirose; Hiroshi Ohta; Yoshitake Nishimune; Hiromitsu Tanaka

  BIOLOGY OF REPRODUCTION, 2007年03月, 査読有り

  
• Successful mouse cloning of an outbred strain by trichostatin A treatment after somatic nuclear transfer

  Satoshi Kishigami; Hong-Thuy Bui; Sayaka Wakayama; Kenzo Tokunaga; Nguyen Van Thuan; Takafusa Hikichi; Eiji Mizutani; Hiroshi Ohta; Rinako Suetsugu; Tetsutaro Sata; Teruhiko Wakayama

  JOURNAL OF REPRODUCTION AND DEVELOPMENT, 2007年02月, 査読有り

  
• Establishment of mouse embryonic stem cell lines from somatic cell nuclei by nuclear transfer into aged, fertilization-failure mouse oocytes

  Sayaka Wakayama; Rinako Suetsugu; Nguyen Van Thuan; Hiroshi Ohta; Satoshi Kishigami; Teruhiko Wakayama

  CURRENT BIOLOGY, 2007年02月, 査読有り

  
• Regulation of chromatin and chromosome morphology by histone H3 modifications in pig oocytes

  Hong-Thuy Bui; Nguyen Van Thuan; Satoshi Kishigami; Sayaka Wakayama; Takafusa Hikichi; Hiroshi Ohta; Eiji Mizutani; Emi Yamaoka; Teruhiko Wakayama; Takashi Miyano

  REPRODUCTION, 2007年02月, 査読有り

  
• Differentiation potential of parthenogenetic embryonic stem cells is improved by nuclear transfer

  Takafusa Hikichi; Sayaka Wakayama; Eiji Mizutani; Yasuhiro Takashima; Satoshi Kishigami; Nguyen Van Thuan; Hiroshi Ohta; Hong Thuy Bui; Shin-Ichi Nishikawa; Teruhiko Wakayama

  STEM CELLS, 2007年01月, 査読有り

  
• Developmental ability of cloned embryos from neural stem cells

  Eiji Mizutani; Hiroshi Ohta; Satoshi Kishigami; Nguyen Van Thuan; Takafusa Hikichi; Sayaka Wakayama; Mitsuko Kosaka; Eimei Sato; Teruhiko Wakayama

  REPRODUCTION, 2006年12月, 査読有り

  
• Carboxypeptidase E in the mouse placenta

  Umashankar Singh; Yang Yu; Elena Kalinina; Toshihiro Konno; Tong Sun; Hiroshi Ohta; Teruhiko Wakayama; Michael J. Soares; Myriam Hemberger; Reinald H. Fundele

  DIFFERENTIATION, 2006年12月, 査読有り

  
• Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts

  Sayaka Wakayama; Martin L. Jakt; Masako Suzuki; Ryoko Araki; Takafusa Hikichi; Satoshi Kishigami; Hiroshi Ohta; Nguyen Van Thuan; Eiji Mizutani; Yuko Sakaide; Sho Senda; Satoshi Tanaka; Mitsuhiro Okada; Masashi Miyake; Masumi Abe; Shin-Ichi Nishikawa; Kunio Shiota; Teruhiko Wakayama

  STEM CELLS, 2006年09月, 査読有り

  
• Injection of somatic cell cytoplasm into oocytes before intracytoplasmic sperm injection impairs full-term development and increases placental weight in mice

  N Van Thuan; S Wakayama; S Kishigami; H Ohta; T Hikichi; E Mizutani; HT Bui; T Wakayama

  BIOLOGY OF REPRODUCTION, 2006年05月, 査読有り

  
• Normal specification of the extraembryonic lineage after somatic nuclear transfer

  S Kishigami; T Hikichi; N Van Thuan; H Ohta; S Wakayama; HT Bui; E Mizutani; T Wakayama

  FEBS LETTERS, 2006年03月, 査読有り

  
• Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer

  S Kishigami; E Mizutani; H Ohta; T Hikichi; N Van Thuan; S Wakayama; HT Bui; T Wakayama

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006年02月, 査読有り

  
• Retinal stem/progenitor properties of iris pigment epithelial cells

  GW Sun; M Asami; H Ohta; J Kosaka; M Kosaka

  DEVELOPMENTAL BIOLOGY, 2006年01月, 査読有り

  
• Epigenetic abnormalities of the mouse paternal zygotic genome associated with microinsemination of round spermatids

  S Kishigami; N Van Thuan; T Hikichi; H Ohta; S Wakayama; E Mizutani; T Wakayama

  DEVELOPMENTAL BIOLOGY, 2006年01月, 査読有り

  
• Production of cloned mice by somatic cell nuclear transfer

  Satoshi Kishigami; Sayaka Wakayama; Nguyen Van Thuan; Hiroshi Ohta; Eiji Mizutani; Takafusa Hikichi; Hong-Thuy Bui; Sebastian Balbach; Atsuo Ogura; Michele Boiani; Teruhiko Wakayama

  NATURE PROTOCOLS, 2006年, 査読有り

  
• Mice cloned by nuclear transfer from somatic and ntES cells derived from the same individuals

  S Wakayama; E Mizutani; S Kishigami; N Van Thuan; H Ohta; T Hikichi; HT Bui; M Miyake; T Wakayama

  JOURNAL OF REPRODUCTION AND DEVELOPMENT, 2005年12月, 査読有り

  
• Expression and functional analysis of genes deregulated in mouse placental overgrowth models: Car2 and Ncam1

  U Singh; T Sun; W Shi; R Schulz; UA Nuber; A Varanou; MC Hemberger; RW Elliott; H Ohta; T Wakayama; R Fundele

  DEVELOPMENTAL DYNAMICS, 2005年12月, 査読有り

  
• Generation of normal progeny by intracytoplasmic sperm injection following grafting of testicular tissue from cloned mice that died postnatally

  H Ohta; T Wakayama

  BIOLOGY OF REPRODUCTION, 2005年09月, 査読有り

  
• Generation of progeny from embryonic stem cells by microinsemination of male germ cells from chimeric mice

  E Mizutani; H Ohta; S Kishigami; N Van Thuan; T Hikichi; S Wakayama; E Sato; T Wakayama

  GENESIS, 2005年09月, 査読有り

  
• Transplantation of spermatogonial stem cells isolated from leukemic mice restores fertility without inducing leukemia

  K Fujita; H Ohta; A Tsujimura; T Takao; Y Miyagawa; S Takada; K Matsumiya; T Wakayama; A Okuyama

  JOURNAL OF CLINICAL INVESTIGATION, 2005年07月, 査読有り

  
• Establishment of male and female nuclear transfer embryonic stem cell lines from different mouse strains and tissues

  S Wakayama; H Ohta; S Kishigami; N Van Thuan; T Hikichi; E Mizutani; M Miyake; T Wakayama

  BIOLOGY OF REPRODUCTION, 2005年04月, 査読有り

  
• Round spermatids stained with MitoTracker can be used to produce offspring more simply

  T Hikichi; S Kishigami; N Van Thuan; H Ohta; E Mizutani; S Wakayama; T Wakayama

  ZYGOTE, 2005年02月, 査読有り

  
• Propagation of an infertile hermaphrodite mouse lacking germ cells by using nuclear transfer and embryonic stem cell technology

  S Wakayama; S Kishigami; N Van Thuan; H Ohta; T Hikichi; E Mizutani; R Yanagimachi; T Wakayama

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005年01月, 査読有り

  
• cAMP-responsive element in TATA-less core promoter is essential for haploid-specific gene expression in mouse testis

  P Somboonthum; H Ohta; S Yamada; M Onishi; A Ike; Y Nishimune; M Nozaki

  NUCLEIC ACIDS RESEARCH, 2005年, 査読有り

  
• Production of offspring from one-day-old oocytes stored at room temperature

  S Wakayama; NV Thuan; S Kishigami; H Ohta; E Mizutani; T Hikichi; M Miyake; T Wakayama

  JOURNAL OF REPRODUCTION AND DEVELOPMENT, 2004年12月, 査読有り

  
• Full-term development of offspring using round spermatids produced ectopically from fetal male germ cells

  H Ohta; T Wakayama

  JOURNAL OF REPRODUCTION AND DEVELOPMENT, 2004年08月, 査読有り

  
• Commitment of fetal male germ cells to spermatogonial stem cells during mouse embryonic development

  H Ohta; T Wakayama; Y Nishimune

  BIOLOGY OF REPRODUCTION, 2004年05月, 査読有り

  
• Rosbin: A novel homeobox-like protein gene expressed exclusively in round spermatids

  T Takahashi; H Tanaka; N Iguchi; K Kitamura; YL Chen; M Maekawa; H Nishimura; H Ohta; Y Miyagawa; K Matsumiya; A Okuyama; Y Nishimune

  BIOLOGY OF REPRODUCTION, 2004年05月, 査読有り

  
• Transient expression analysis of the mouse ornithine decarboxylase antizyme haploid-specific promoter using in vivo electroporation

  A Ike; H Ohta; M Onishi; N Iguchi; Y Nishimune; M Nozaki

  FEBS LETTERS, 2004年02月, 査読有り

  
• Proliferation and differentiation of spermatogonial stem cells in the W/W-v mutant mouse testis

  H Ohta; A Tohda; Y Nishimune

  BIOLOGY OF REPRODUCTION, 2003年12月, 査読有り

  
• Methylation of CpG dinucleotides in the open reading frame of a testicular germ cell-specific intronless gene, Tact1/Actl7b, represses its expression in somatic cells

  M Hisano; H Ohta; Y Nishimune; M Nozaki

  NUCLEIC ACIDS RESEARCH, 2003年08月, 査読有り

  
• Functional analysis of the p53 gene in apoptosis induced by heat stress or loss of stem cell factor signaling in mouse male germ cells

  H Ohta; S Aizawa; Y Nishimune

  BIOLOGY OF REPRODUCTION, 2003年06月, 査読有り

  
• Nectin couples cell-cell adhesion and the actin scaffold at heterotypic testicular junctions

  K Ozaki-Kuroda; H Nakanishi; H Ohta; H Tanaka; H Kurihara; S Mueller; K Irie; W Ikeda; T Sakai; E Wimmer; Y Nishimune; Y Takai

  CURRENT BIOLOGY, 2002年07月, 査読有り

  
• Development of the stages of the cycle in mouse seminiferous epithelium after transplantation of green fluorescent protein-labeled spermatogonial stem cells

  S Ventela; H Ohta; M Parvinen; Y Nishimune

  BIOLOGY OF REPRODUCTION, 2002年05月, 査読有り

  
• Homeostatic regulation of germinal stem cell proliferation by the GDNF/FSH pathway

  Y Tadokoro; K Yomogida; H Ohta; A Tohda; Y Nishimune

  MECHANISMS OF DEVELOPMENT, 2002年04月, 査読有り

  
• Defect in germ cells, not in supporting cells, is the cause of male infertility in the jsd mutant mouse: proliferation of spermatogonial stem cells without differentiation

  H Ohta; K Yomogida; Y Tadokoro; A Tohda; K Dohmae; Y Nishimune

  INTERNATIONAL JOURNAL OF ANDROLOGY, 2001年02月, 査読有り

  
• Isolation and characterization of a haploid germ cell-specific novel complementary deoxyribonucleic acid; Testis-specific homologue of succinyl CoA: 3-oxo acid CoA transferase

  M Koga; H Tanaka; K Yomogida; M Nozaki; J Tsuchida; H Ohta; Y Nakamura; K Masai; Y Yoshimura; M Yamanaka; N Iguchi; H Nojima; K Matsumiya; A Okuyama; Y Nishimune

  BIOLOGY OF REPRODUCTION, 2000年12月, 査読有り

  
• Molecular cloning and characterization of phosphatidylcholine transfer protein-like protein gene expressed in murine haploid germ cells

  M Yamanaka; M Koga; H Tanaka; Y Nakamura; H Ohta; K Yomogida; J Tsuchida; N Iguchi; H Nojima; M Nozaki; K Matsumiya; A Okuyama; K Toshimori; Y Nishimune

  BIOLOGY OF REPRODUCTION, 2000年06月, 査読有り

  
• Regulation of proliferation and differentiation in spermatogonial stem cells: the role of c-kit and its ligand SCF

  H Ohta; K Yomogida; K Dohmae; Y Nishimune

  DEVELOPMENT, 2000年05月, 査読有り

  
• Real-time observation of transplanted 'green germ cells': Proliferation and differentiation of stem cells

  H Ohta; K Yomogida; S Yamada; M Okabe; Y Nishimune

  DEVELOPMENT GROWTH & DIFFERENTIATION, 2000年04月, 査読有り

  

MISC

• 核移植胚への同一ntES細胞注入によるクローン作出効率改善の試み

  水谷英二; 岸上哲士; NGUYEN Van Thuan; 大田浩; 引地貴亮; BUI Hong Thuy; 若山清香; 若山照彦

  J Reprod Dev, 2006年08月25日

  
• 極体および初期胚割球からのES細胞樹立の試み

  若山清香; 岸上哲士; 坂出裕子; NGUYEN Van Thuan; 大田浩; 引地貴亮; 水谷英二; BUI Hong Thuy; 若山照彦

  J Reprod Dev, 2006年08月25日

  

外部資金：科学研究費補助金

• 試験管内において卵子形成の支持が可能な卵巣体細胞の新規培養法の確立

  挑戦的研究(萌芽)

  中区分48:生体の構造と機能およびその関連分野

  京都大学

  大田 浩

  自 2020年07月30日, 至 2023年03月31日, 完了

  卵子形成;再構成卵巣;雌性生殖巣体細胞;前顆粒膜細胞;細胞培養;PGCLC;マウス;生殖巣;体細胞;培養;支持細胞;始原生殖細胞

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K21500/
• 雄性と妊孕性獲得を担う遺伝子発現カスケードの解明

  基盤研究(A)

  中区分44:細胞レベルから個体レベルの生物学およびその関連分野

  自治医科大学

  本多 新

  自 2020年04月01日, 至 2025年03月31日, 交付

  性決定;絶滅危惧種;iPS細胞;生殖巣;生殖細胞;アマミトゲネズミ;胚盤胞補完法;マウス;ラット;生殖細胞体外再構築

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20H00469/
• ヒト始原生殖細胞様細胞の卵母細胞誘導法の確立

  基盤研究(B)

  小区分52050:胎児医学および小児成育学関連

  京都大学

  大田 浩

  自 2019年04月01日, 至 2023年03月31日, 完了

  卵原細胞;卵母細胞;卵子形成;再構成卵巣;始原生殖細胞;試験管内誘導;ヒト;多能性幹細胞;始原生殖細胞様細胞;試験管内;胎生期生殖巣;iPS細胞;in vitro;細胞分化

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H03618/
• 試験管内におけるES細胞から始原生殖細胞の効率的な分化誘導方法の開発

  挑戦的萌芽研究

  京都大学

  大田 浩

  自 2015年04月01日, 至 2017年03月31日, 完了

  始原生殖細胞;ES細胞;EpiLC;分化誘導;増殖;分化

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K14365/
• ES細胞由来始原生殖細胞の増殖・分化機構の解明

  若手研究(A)

  京都大学

  大田 浩

  自 2015年04月01日, 至 2019年03月31日, 完了

  始原生殖細胞;ES細胞;配偶子形成;増殖;分化;細胞培養;培養;細胞増殖;細胞分化;試験管内

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15H05636/
• ＥＳ細胞由来始原生殖細胞を用いた新規発生工学技術の開発

  若手研究(A)

  京都大学

  大田 浩

  自 2012年04月01日, 至 2015年03月31日, 完了

  始原生殖細胞;ES細胞;小分子化合物;増殖・分化;精子形成;卵子形成;分化誘導;培養;chemical libray;high-throughput screening

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24680045/
• ヒト卵原細胞から卵子への試験管内誘導系の確立

  基盤研究(B)

  小区分56040:産婦人科学関連

  京都大学

  大田 浩

  自 2023年04月01日, 至 2027年03月31日, 交付

  卵巣;卵胞;卵母細胞;培養;ヒト

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23H03042/
• ヒト卵原細胞から卵子への試験管内誘導系の確立

  基盤研究(B)

  小区分56040:産婦人科学関連

  京都大学

  大田 浩

  自 2023年04月01日, 至 2027年03月31日, 交付

  卵原細胞;卵子;卵胞;ヒト;培養;原始卵胞;卵子形成;卵巣;卵母細胞

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K27733/

大学運営

部局運営(役職等)

• 自 2023年04月01日, 至 2024年03月31日

  医学部構内実験排水系管理委員会委員
• 自 2022年04月01日, 至 2023年03月31日

  医学部構内実験排水系管理委員会委員
• 自 2013年04月01日, 至 2019年03月31日

  医学図書館図書連絡委員
• 自 2015年04月01日, 至 2019年03月31日

  動物実験施設運営委員会
• 自 2021年04月01日, 至 2022年03月31日

  医学部構内実験排水系管理委員会委員