Basic Information

Academic Degree

• 修士（農学）(京都工芸繊維大学)
• 博士（医学）(京都大学)

Research History

• From Jan. 2023, To Present

  京都大学医生物学研究所, 細胞生物学研究部門, 特定准教授
• From Oct. 2021, To Dec. 2022

  京都大学大学院生命科学研究科, 脳機能発達再生生物学, 講師
• From Apr. 2018, To Sep. 2021

  Kyoto University, Graduate School of Biostudies, 特定助教
• From Jul. 2013, To Mar. 2018

  Kyoto University, Graduate School of Medicine, 特定助教
• From Apr. 2011, To Jun. 2013

  日本学術振興会, 特別研究員PD

Profile

• Profile

  2004年 京都工芸繊維大学繊維学部応用生物学科卒業
  2006年 京都工芸繊維大学大学院工芸科学研究科修士課程応用生物学専攻修了
  2010年 京都大学大学院医学研究科博士課程医学専攻修了
  2010年 博士（医学）（京都大学）取得
  
  2008年 日本学術振興会特別研究員(DC2) 京都大学大学院医学研究科
  2010年 国立精神・神経医療研究センター 神経研究所 流動研究員
  2011年 日本学術振興会特別研究員(PD) 国立精神・神経医療研究センター 神経研究所
  2013年 京都大学大学院医学研究科メディカルイノベーションセンターSKプロジェクト 特定助教
  2018年 京都大学大学院生命科学研究科 特定助教

  
  

  2021年 京都大学大学院生命科学研究科 講師

  
  

  2023年 京都大学医生物学研究所 特定准教授

ID,URL

• J-Global ID

  201101009464292229

researchmap URL

• https://researchmap.jp/myamada

Research

Research Topics, Overview of the research

• Research Topics

  The analysis of neural stem cell differentiation by using the novel optogenetics

• Overview of the research

  The mammalian brain consists of a complex ensemble of neurons and glial cells. Their production during development and remodelling is tightly controlled by various regulatory mechanisms in neural stem cells. We have recently developed a photoactivatable-Tet system for precise temporal control of gene expression at single-cell resolution. By applying the novel light-inducible gene expression system, I will regulate the dynamic expression changes of several transcription factors, and I will investigate the mechanism of neural stem cell differentiation.

Research Interests

• Oligodendrocyte
• Gene expression profile
• Optogenetics

Papers

• Enhancement of Vivid-based photo-activatable Gal4 transcription factor in mammalian cells

  Shinji C. Nagasaki; Tomonori D. Fukuda; Mayumi Yamada; Yusuke III Suzuki; Ryo Kakutani; Adam T. Guy; Itaru Imayoshi

  Cell Structure and Function, 2023

  Access this paper :

  
• Separate optogenetic manipulation of Nerve/glial antigen 2 (NG2) glia and mural cells using the NG2 promoter

  Mitsuhiro Oishi; Stefan Passlick; Yoshihiko Yamazaki; Miyuki Unekawa; Ruka Adachi; Mayumi Yamada; Itaru Imayoshi; Yoshifumi Abe; Christian Steinhäuser; Kenji F. Tanaka

  Glia, 27 Sep. 2022, Peer-reviewed

  
• Cell cycle arrest determines adult neural stem cell ontogeny by an embryonic Notch-nonoscillatory Hey1 module

  Yujin Harada; Mayumi Yamada; Itaru Imayoshi; Ryoichiro Kageyama; Yutaka Suzuki; Takaaki Kuniya; Shohei Furutachi; Daichi Kawaguchi; Yukiko Gotoh

  Nature Communications, 21 Dec. 2021, Peer-reviewed

  
• Functional rejuvenation of aged neural stem cells by Plagl2 and anti-Dyrk1a activity

  Takashi Kaise; Masahiro Fukui; Risa Sueda; Wenhui Piao; Mayumi Yamada; Taeko Kobayashi; Itaru Imayoshi; Ryoichiro Kageyama

  Genes & Development, 01 Jan. 2022, Peer-reviewed

  Access this paper :

  
• An Optimized Preparation Method for Long ssDNA Donors to Facilitate Quick Knock-In Mouse Generation

  Yukiko U. Inoue; Yuki Morimoto; Mayumi Yamada; Ryosuke Kaneko; Kazumi Shimaoka; Shinji Oki; Mayuko Hotta; Junko Asami; Eriko Koike; Kei Hori; Mikio Hoshino; Itaru Imayoshi; Takayoshi Inoue

  Cells, 30 Apr. 2021, Peer-reviewed

  
• Down syndrome cell adhesion molecule like-1 (DSCAML1) links the GABA system and seizure susceptibility

  Yoneko Hayase; Shigeru Amano; Koichi Hashizume; Takashi Tominaga; Hiroyuki Miyamoto; Yukie Kanno; Yukiko Ueno-Inoue; Takayoshi Inoue; Mayumi Yamada; Shigehiro Ogata; Shabeesh Balan; Ken Hayashi; Yoshiki Miura; Kentaro Tokudome; Yukihiro Ohno; Takuma Nishijo; Toshihiko Momiyama; Yuchio Yanagawa; Akiko Takizawa; Tomoji Mashimo; Tadao Serikawa; Akihiro Sekine; Eiji Nakagawa; Eri Takeshita; Takeo Yoshikawa; Chikako Waga; Ken Inoue; Yu-ichi Goto; Yoichi Nabeshima; Nobuo Ihara; Kazuhiro Yamakawa; Shinichiro Taya; Mikio Hoshino

  Acta Neuropathologica Communications, Dec. 2020

  
• Optimization of Light-Inducible Gal4/UAS Gene Expression System in Mammalian Cells

  Mayumi Yamada; Shinji C. Nagasaki; Yusuke Suzuki; Yukinori Hirano; Itaru Imayoshi

  iScience, Sep. 2020, Lead author

  Access this paper :

  
• Light-mediated control of Gene expression in mammalian cells

  Mayumi Yamada; Shinji C. Nagasaki; Takeaki Ozawa; Itaru Imayoshi

  Neuroscience Research, Mar. 2020, Peer-reviewed

  Access this paper :

  
• Regulatory Mechanism of Neural Progenitor Cells Revealed by Optical Manipulation of Gene Expressions

  Itaru Imayoshi; Mayumi Yamada; Yusuke Suzuki

  Make Life Visible, 2020, Peer-reviewed

  
• Enhanced lysosomal degradation maintains the quiescent state of neural stem cells

  Taeko Kobayashi; Wenhui Piao; Toshiya Takamura; Hiroshi Kori; Hitoshi Miyachi; Satsuki Kitano; Yumiko Iwamoto; Mayumi Yamada; Itaru Imayoshi; Seiji Shioda; Andrea Ballabio; Ryoichiro Kageyama

  Nature Communications, Nov. 2019, Peer-reviewed

  Access this paper :

  
• Light Control of the Tet Gene Expression System in Mammalian Cells

  Mayumi Yamada; Yusuke Suzuki; Shinji C. Nagasaki; Hiroyuki Okuno; Itaru Imayoshi

  Cell Reports, Oct. 2018, Peer-reviewed

  
• Precerebellar nuclei

  Mayumi Yamada; Mikio Hoshino

  Essentials of Cerebellum and Cerebellar Disorders: A Primer for Graduate Students, 01 Jan. 2016, Peer-reviewed

  
• Spatiotemporal regulation of neural progenitor identities by transcription factors

  Hoshino Mikio; Seto Yusuke; Nakatani Tomoya; Kawaguchi Yoshiya; Ikenaka Kazuhiro; Takebayashi Hirohide; Ono Yuichi; Yamada Mayumi

  INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, Dec. 2015, Peer-reviewed

  
• Specification of Spatial Identities of Cerebellar Neuron Progenitors by Ptf1a and Atoh1 for Proper Production of GABAergic and Glutamatergic Neurons

  Mayumi Yamada; Yusuke Seto; Shinichiro Taya; Tomoo Owa; Yukiko U. Inoue; Takayoshi Inoue; Yoshiya Kawaguchi; Yo-ichi Nabeshima; Mikio Hoshino

  JOURNAL OF NEUROSCIENCE, Apr. 2014, Peer-reviewed

  
• Temporal identity transition from Purkinje cell progenitors to GABAergic interneuron progenitors in the cerebellum

  Yusuke Seto; Tomoya Nakatani; Norihisa Masuyama; Shinichiro Taya; Minoru Kumai; Yasuko Minaki; Akiko Hamaguchi; Yukiko U. Inoue; Takayoshi Inoue; Satoshi Miyashita; Tomoyuki Fujiyama; Mayumi Yamada; Heather Chapman; Kenneth Campbell; Mark A. Magnuson; Christopher V. Wright; Yoshiya Kawaguchi; Kazuhiro Ikenaka; Hirohide Takebayashi; Shin'ichi Ishiwata; Yuichi Ono; Mikio Hoshino

  NATURE COMMUNICATIONS, Feb. 2014, Peer-reviewed

  
• Specification of cerebellar and precerebellar neurons

  Mikio Hoshino; Yusuke Seto; Mayumi Yamada

  Handbook of the Cerebellum and Cerebellar Disorders, 01 Jan. 2013, Peer-reviewed

  
• Thioredoxin Mediates Oxidation-Dependent Phosphorylation of CRMP2 and Growth Cone Collapse

  Akifumi Morinaka; Mayumi Yamada; Rurika Itofusa; Yosuke Funato; Yuta Yoshimura; Fumio Nakamura; Takeshi Yoshimura; Kozo Kaibuchi; Yoshio Goshima; Mikio Hoshino; Hiroyuki Kamiguchi; Hiroaki Miki

  SCIENCE SIGNALING, Apr. 2011, Peer-reviewed

  
• Function and mutual expression control of bHLH transcription factors, Ptf1a and Atoh1, in the developing cerebellum

  Yamada Mayumi; Taya Shinichiro; Owa Tomoo; Kawaguchi Yoshiya; Nabeshima Yo-ichi; Hoshino Mikio

  NEUROSCIENCE RESEARCH, 2010, Peer-reviewed

  
• Inhibitory and excitatory subtypes of cochlear nucleus neurons are defined by distinct bHLH transcription factors, Ptf1a and Atoh1

  Tomoyuki Fujiyama; Mayumi Yamada; Mami Terao; Toshio Terashima; Hiroyuki Hioki; Yukiko U. Inoue; Takayoshi Inoue; Norihisa Masuyama; Kunihiko Obata; Yuchio Yanagawa; Yoshiya Kawaguchi; Yo-ichi Nabeshima; Mikio Hoshino

  DEVELOPMENT, Jun. 2009, Peer-reviewed

  
• IgLON cell adhesion molecule Kilon is a crucial modulator for synapse number in hippocampal neurons

  Takashi Hashimoto; Mayumi Yamada; Shohei Maekawa; Toshihiro Nakashima; Seiji Miyata

  BRAIN RESEARCH, Aug. 2008, Peer-reviewed

  
• Genetic studies on the development of cochlear nucleus neurons using Ptf1a-cre knock-in mice

  Fujiyama Tomoyuki; Yamada Mayumi; Terao Mami; Terashima Toshio; Yanagawa Yuchio; Obata Kunihiko; Kawaguchi Yoshiya; Nabeshima Yo-Ichi; Hoshino Mikio

  NEUROSCIENCE RESEARCH, 2008, Peer-reviewed

  
• Origin of climbing fiber neurons and their developmental dependence on Ptf1a

  Mayumi Yamada; Mami Terao; Toshio Terashima; Tomoyuki Fujiyama; Yoshiya Kawaguchi; Yo-ichi Nabeshima; Mikio Hoshino

  JOURNAL OF NEUROSCIENCE, Oct. 2007, Peer-reviewed

  
• Synaptic adhesion molecule OBCAM; synaptogenesis and dynamic internalization

  Mayumi Yamada; Takashi Hashimoto; Noriko Hayashi; Maiko Higuchi; Akira Murakami; Toshihiro Nakashima; Shohei Maekawa; Seiji Miyata

  BRAIN RESEARCH, Aug. 2007, Peer-reviewed

  
• Origin of climbing fiber neurons and their developmental dependence on Ptf1a

  Yamada Mayumi; Terao Mami; Terashima Toshio; Fujiyama Tomoyuki; Kawaguchi Yoshiya; Nabeshima Yo-Ichi; Hoshino Mikio

  NEUROSCIENCE RESEARCH, 2007, Peer-reviewed

  
• Neuronal expression of the chondroitin sulfate proteoglycans receptor-type protein-tyrosine phosphatase beta and phosphacan

  N Hayashi; S Miyata; M Yamada; K Kamei; A Oohira

  NEUROSCIENCE, 2005, Peer-reviewed

  

Misc.

• 生後脳・成体脳におけるニューロン新生と神経幹細胞の制御機構

  今吉 格; 山田 真弓

  月刊 細胞, Jun. 2018

  
• Clinical Neuroscience

  今吉格; 山田真弓

  Clinical Neuroscience, 2018, Invited

  
• Spine-specific cell adhesion molecule OBCAM is crucial for synapse formation

  Mayumi Yamada; Shohei Maekawa; Seiji Miyata

  NEUROSCIENCE RESEARCH, 2006

  
• Neuronal expression of the chondroitin sulfate proteoglycans RPTP beta and phosphacan

  N Hayashi; S Miyata; M Yamada; K Kamei; A Oohira

  GLYCOBIOLOGY, Nov. 2004

  

Presentations

• Analysis of regulatory mechanism of neural stem cells by optical manipulation of bHLH transcription factor expressions

  Mayumi Yamada

  The 29th East Asia Joint Symposium, 26 Nov. 2023, Invited

  
• 青色光を用いた遺伝子発現の光操作技術の開発

  Mayumi Yamada

  第45回日本分子生物学会年会, 02 Dec. 2022, Invited

  
• Analysis of neural stem cell regulatory mechanisms using optogenetics

  Mayumi Yamada

  第16回生命医科学研究所ネットワーク国際シンポジウム, 12 Nov. 2021, Invited

  
• Analysis of neural stem cell regulatory mechanism using optogenetics

  山田真弓

  京都大学大学院医学研究科 大学院教育コース「再生医療・臓器再建医学コース」, 09 Jul. 2021, Invited

  
• 「青色光」による遺伝子スイッチの制御

  山田真弓

  バイオインダストリー協会“未来へのバイオ技術”勉強会, Apr. 2021, Invited

  
• Dynamic transcriptional regulation of neural stem cells by bHLH factors

  山田真弓

  第63回日本神経化学会大会シンポジウム, Sep. 2020, Invited

  
• Optimization of the photo-activatable gene expression system in mammalian cells

  Mayumi Yamada

  The 17th International Joint Mini-Symposium on Molecular and Cell Biology, 30 Jun. 2018, Invited

  
• New optical tools for manipulating gene expressions in neural stem cells

  Mayumi Yamada

  第9回光操作研究会（2017）, Nov. 2017, Invited

  
• Impairment of the hippocampal postnatal neurogenesis and its involvement in the neurodevelopmental disorder

  Mayumi Yamada; Itaru Imayoshi

  Neuro2016, 2016, Invited

  

Books and Other Publications

• 生後脳・成体脳におけるニューロン新生と神経幹細胞の制御機構

  今吉 格; 山田 真弓, Joint work

  月刊 細胞, Jun. 2018, Not refereed

  
• 遺伝子発現の光制御

  今吉 格; 山田 真弓, Joint work

  Clinical Neuroscience 36(8), 2018, Not refereed

  
• Anatomy and histology of the cerebellum; Pre-cerebellar nuclei

  Mayumi Yamada M; Mikio Hoshino, Joint work

  Essentials of cerebellum and cerebellar disorders, Springer, 2016, Not refereed

  
• Specification of Cerebellar and Precerebellar Neurons

  Mikio Hoshino; Satoshi Miyashita; Yusuke Seto; Mayumi Yamada, Joint work

  Handbook of the Cerebellum and Cerebellar Disorders, Springer, Not refereed

  

Industrial Property Rights

• 特願K108P26JP, 光活性化可能なＴｅｔ発現制御システム

  今吉 格; 山田 真弓; 鈴木 裕輔; 長崎真治

  

External funds: Kakenhi

• Analysis of neural stem cell regulatory mechanisms using a novel light manipulation system

  Grant-in-Aid for Scientific Research (C)

  Basic Section 44020:Developmental biology-related

  Kyoto University

  Mayumi Yamada

  From 01 Apr. 2020, To 31 Mar. 2023, Project Closed

  神経幹細胞;光操作;ニューロン分化;転写因子;遺伝子発現;細胞増殖;RNA-seq解析;分化運命決定因子;RNAシークエンス解析;遺伝子ネットワーク;光遺伝学;細胞分化;Tetシステム;神経発生;RNAシークエンス;RNA-seq;ニューロン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K06667/
• Finding novel adult neurogenesis-related genes in the mouse brain

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  Mayumi Yamada

  From 01 Apr. 2017, To 31 Mar. 2020, Project Closed

  神経幹細胞;遺伝子発現プロファイル;RNAシークエンス;神経新生;光遺伝学;海馬歯状回;側脳室周囲;転写因子;ニューロン新生;FACS;光作動性転写因子;遺伝子発現プロファイリング;神経発生;トランスジェニックマウス

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K14950/
• Analysis of the temporal gene expression changes in neural stem cells during brain maturation and aging

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  Mayumi Yamada

  From 01 Apr. 2015, To 31 Mar. 2018, Project Closed

  神経新生;神経発生;RNAシークエンス;遺伝子発現プロファイリング;海馬歯状回;側脳室周囲;光遺伝学;神経幹細胞;ニューロン新生;遺伝子発現プロファイル;成体脳

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K18362/
• 小脳をモデル系とした、bHLH型転写因子による神経細胞多様性獲得機構の解明

  Grant-in-Aid for Young Scientists (B)

  National Center of Neurology and Psychiatry

  山田 真弓

  From 28 Apr. 2011, To 31 Mar. 2012, Declined

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23700407/
• 光操作技術とNGS解析を用いた神経幹細胞の制御メカニズムの解析

  Grant-in-Aid for Scientific Research (C)

  Basic Section 44020:Developmental biology-related

  Kyoto University

  山田 真弓

  From 01 Apr. 2023, To 31 Mar. 2026, Granted

  神経幹細胞;光操作技術;NGS;転写因子;ニューロン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K05792/

Education

Teaching subject(s)

• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Course in Multicellular Network

  1030, Spring, Graduate School of Biostudies, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar C

  2008, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar A

  2006, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar D

  2009, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Experiments in Systemic Life Sciences

  5013, Fall, Graduate School of Biostudies, 4
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar D

  2009, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar C

  2008, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar B

  2007, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar B

  2007, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Experiments in Systemic Life Sciences

  5013, Spring, Graduate School of Biostudies, 4
• From 01 Apr. 2022, To 31 Mar. 2023

  Introduction to Biology and Life Science

  N484, Spring, Institute for Liberal Arts and Sciences, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar A

  2006, Spring, Graduate School of Biostudies, 5

Administration

Faculty management (title, position)

• From 01 Apr. 2023

  共同利用機器等委員会　委員
• From 01 Apr. 2023

  化学物質管理委員会　委員
• From 01 Oct. 2021

  情報発信・ホームページ委員会