Basic Information

Affiliated programs (koza)

• Graduate School of Biostudies, 統合生命科学専攻 微細構造ウイルス学分野, 准教授

Professional Memberships

• From 2022, To Present

  Protein Science Society of Japan
• From 2018, To Present

  THE BIOPHYSICAL SOCIETY OF JAPAN
• From 2010, To Present

  THE JAPANESE SOCIETY FOR VIROLOGY

Academic Degree

• 博士（医学）(東京大学)

Research History

• From Apr. 2025, To Present

  Kyoto University, Institute for Life and Medical Sciences/Graduate School of Biostudies, Associate professor
• From Jan. 2023, To Mar. 2025

  Kyoto University, Hakubi Center/Institute for Life and Medical Sciences/Graduate School of Biostudies, Associate professor
• From Jan. 2020, To Dec. 2022

  Kyoto University, Hakubi Center/Institute for Life and Medical Sciences, Program-Specific Assistant Professor
• From Jan. 2020, To Mar. 2021

  Osaka University, Institute for Protein Research, 招へい教員
• From Feb. 2019, To Dec. 2019

  Osaka University, Institute for Protein Research, Specially Appointed Assistant Professor
• From Feb. 2018, To Jan. 2019

  Osaka University, Institute for Protein Research, Specially Appointed Researcher
• From Apr. 2014, To Jan. 2018

  Okinawa Institute of Science and Technology, Molecular Cryo-electron Microscopy Unit, Postdoctoral Scholar
• From Apr. 2012, To Mar. 2014

  Japan Society for the Promotion Science, Research Fellow (DC2)

Language of Instruction

• Japanese
• English

ID,URL

• ORCID ID

  0000-0001-6861-4840

• J-Global ID

  201501018306606622

• Researcher number

  00734469

• External link

  https://kaken.nii.ac.jp/d/r/00734469.ja.html

Website(s) (URL(s))

• https://www.notion.so/Yukihiko-Sugita-DVM-PhD-c3ade339e55c47b4ad451016c4a89215

researchmap URL

• https://researchmap.jp/y-sugita

Research

Research Interests

• mononegavirus
• structural biology
• Cryo-electron microscopy
• Virology
• Veterinary Medicine
• Ebola virus
• influenza virus

Research Areas

• Life sciences, Structural biochemistry
• Life sciences, Morphology, anatomy
• Life sciences, Virology
• Life sciences, Veterinary medicine

Papers

• Structural analysis reveals how tetrameric tyrosine-phosphorylated STAT1 is targeted by the rabies virus P-protein

  Yuichiro Takekawa; Yukihiko Sugita; Xiaomei Sun; Toyoyuki Ose; min yao; Miku Minami; Tomo Nomai; Nana Yabuno; Gregory Moseley; Aoi Sugiyama; Mika Hirose; Shiho Takei; Satomi Inaba, Satomi Inaba-Inoue; Kaito Ugajin; Paul R. Gooley; Xinxin Jiang; katsumi maenaka; Shunsuke Kita; Mina Sasaki

  Science Signaling, 18 Mar. 2025

  
• Structural basis for Ebola virus nucleocapsid assembly and function regulated by VP24

  Yoko Fujita-Fujiharu; Shangfan Hu; Ai Hirabayashi; Yuki Takamatsu; Yen Ni Ng; Kazuya Houri; Yukiko Muramoto; Masahiro Nakano; Yukihiko Sugita; Takeshi Noda

  Nature Communications, 10 Mar. 2025

  
• Neurotransmitter recognition by human vesicular monoamine transporter 2.

  Dohyun Im; Mika Jormakka; Narinobu Juge; Jun-Ichi Kishikawa; Takayuki Kato; Yukihiko Sugita; Takeshi Noda; Tomoko Uemura; Yuki Shiimura; Takaaki Miyaji; Hidetsugu Asada; So Iwata

  Nature communications, 16 Sep. 2024

  Access this paper :

  
• Induction of IGHV3-53 public antibodies with broadly neutralising activity against SARS-CoV-2 including Omicron subvariants in a Delta breakthrough infection case

  Takeo Kuwata; Yu Kaku; Shashwata Biswas; Kaho Matsumoto; Mikiko Shimizu; Yoko Kawanami; Ryuta Uraki; Kyo Okazaki; Rumi Minami; Yoji Nagasaki; Mami Nagashima; Isao Yoshida; Kenji Sadamasu; Kazuhisa Yoshimura; Mutsumi Ito; Maki Kiso; Seiya Yamayoshi; Masaki Imai; Terumasa Ikeda; Kei Sato; Mako Toyoda; Takamasa Ueno; Takako Inoue; Yasuhito Tanaka; Kanako Tarakado Kimura; Takao Hashiguchi; Yukihiko Sugita; Takeshi Noda; Hiroshi Morioka; Yoshihiro Kawaoka; Shuzo Matsushita; Jumpei Ito; Naoko Misawa; Arnon Plianchaisuk; Ziyi Guo; Alfredo Hina; Keiya Uriu; Kaoru Usui; Wilaiporn Saikruang; Spyridon Lytras; Ryo Yoshimura; Shusuke Kawakubo; Luca Nishimura; Yusuke Kosugi; Shigeru Fujita; Luo Chen; Jarel Elgin M. Tolentino; Lin Pan; Wenye Li; Maximilian Stanley Yo; Kio Horinaka

  eBioMedicine, Nov. 2024

  
• Step-by-Step Maturation Mechanism of Binary Toxin Pore Revealed by Cryo-EM Analysis

  Tomohito Yamada; Yukihiko Sugita; Toru Yoshida; Takeshi Noda; Hideaki Tsuge

  10 Oct. 2024

  
• Structural and Dynamic Insights into the Biased Signaling Mechanism of the Human Kappa Opioid Receptor

  Chiyo Suno-Ikeda; Ryo Nishikawa; Riko Suzuki; Seiya Iwata; Tomoyo Takai; Takaya Ogura; Mika Hirose; Akitoshi Inoue; Eri Asai; Ryoji Kise; Yukihiko Sugita; Takayuki Kato; Hiroshi Nagase; Tsuyoshi Saitoh; Kota Katayama; Asuka Inoue; Hideki Kandori; Takuya Kobayashi; Ryoji Suno

  15 Apr. 2024

  
• Protein design of two-component tubular assemblies like cytoskeletons

  Yuta Suzuki; Masahiro Noji; Yukihiko Sugita; Yosuke Yamazaki; Makito Miyazaki

  21 Oct. 2024

  
• Structural basis of Ebola virus nucleocapsid assembly and functions

  Yoko Fujita-Fujiharu; Shangfan Hu; Ai Hirabayashi; Yuki Takamatsu; Yen Ni Ng; Kazuya Houri; Yukiko Muramoto; Masahiro Nakano; Yukihiko Sugita; Takeshi Noda

  bioRxiv, 19 Feb. 2024

  
• Structural insights into the agonists binding and receptor selectivity of human histamine H4 receptor.

  Dohyun Im; Jun-Ichi Kishikawa; Yuki Shiimura; Hiromi Hisano; Akane Ito; Yoko Fujita-Fujiharu; Yukihiko Sugita; Takeshi Noda; Takayuki Kato; Hidetsugu Asada; So Iwata

  Nature communications, 20 Oct. 2023

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• Cryoelectron microscopic structure of the nucleoprotein–RNA complex of the European filovirus, Lloviu virus

  Shangfan Hu; Yoko Fujita-Fujiharu; Yukihiko Sugita; Lisa Wendt; Yukiko Muramoto; Masahiro Nakano; Thomas Hoenen; Takeshi Noda

  PNAS Nexus, 03 Apr. 2023

  Access this paper :

  
• Structural insights into the G protein selectivity revealed by the human EP3-Gi signaling complex.

  Ryoji Suno; Yukihiko Sugita; Kazushi Morimoto; Hiroko Takazaki; Hirokazu Tsujimoto; Mika Hirose; Chiyo Suno-Ikeda; Norimichi Nomura; Tomoya Hino; Asuka Inoue; Kenji Iwasaki; Takayuki Kato; So Iwata; Takuya Kobayashi

  Cell reports, 13 Sep. 2022

  Access this paper :

  
• Structural insight into Marburg virus nucleoprotein-RNA complex formation.

  Yoko Fujita-Fujiharu; Yukihiko Sugita; Yuki Takamatsu; Kazuya Houri; Manabu Igarashi; Yukiko Muramoto; Masahiro Nakano; Yugo Tsunoda; Ichiro Taniguchi; Stephan Becker; Takeshi Noda

  Nature communications, 04 Mar. 2022, Peer-reviewed

  Access this paper :

  
• Functional Analysis of the GPI Transamidase Complex by Screening for Amino Acid Mutations in Each Subunit.

  Si-Si Liu; Fei Jin; Yi-Shi Liu; Yoshiko Murakami; Yukihiko Sugita; Takayuki Kato; Xiao-Dong Gao; Taroh Kinoshita; Motoyuki Hattori; Morihisa Fujita

  Molecules (Basel, Switzerland), 08 Sep. 2021, Peer-reviewed

  
• Ultrastructure of influenza virus ribonucleoprotein complexes during viral RNA synthesis.

  Masahiro Nakano; Yukihiko Sugita; Noriyuki Kodera; Sho Miyamoto; Yukiko Muramoto; Matthias Wolf; Takeshi Noda

  Communications biology, 09 Jul. 2021, Peer-reviewed

  Access this paper :

  
• Cryo-EM structure of the Ebola virus nucleoprotein-RNA complex at 3.6 Å resolution.

  Yukihiko Sugita; Hideyuki Matsunami; Yoshihiro Kawaoka; Takeshi Noda; Matthias Wolf

  Nature, Nov. 2018, Peer-reviewed

  
• Lung-Derived Exosomal miR-483-3p Regulates the Innate Immune Response to Influenza Virus Infection.

  Tadashi Maemura; Satoshi Fukuyama; Yukihiko Sugita; Tiago J S Lopes; Tomomi Nakao; Takeshi Noda; Yoshihiro Kawaoka

  The Journal of infectious diseases, 11 Apr. 2018, Peer-reviewed

  
• Structural Basis of Heterochromatin Formation by Human HP1.

  Machida S; Takizawa Y; Ishimaru M; Sugita Y; Sekine S; Nakayama J; Wolf M; Kurumizaka H

  Molecular Cell, 01 Feb. 2018, Peer-reviewed

  
• Structural Basis of Heterochromatin Formation by Human HP1

  Shinichi Machida; Yoshimasa Takizawa; Masakazu Ishimaru; Yukihiko Sugita; Satoshi Sekine; Jun-ichi Nakayama; Matthias Wolf; Hitoshi Kurumizaka

  Molecular Cell, Feb. 2018, Peer-reviewed

  
• Genetic and antigenic characterization of bovine viral diarrhea viruses isolated from cattle in Hokkaido, Japan

  Yuri Abe; Tomokazu Tamura; Shiho Torii; Shiho Wakamori; Makoto Nagai; Kazuya Mitsuhashi; Junki Mine; Yuri Fujimoto; Naofumi Nagashima; Fumi Yoshino; Yukihiko Sugita; Takushi Nomura; Masatoshi Okamatsu; Hiroshi Kida; Yoshihiro Sakoda

  JOURNAL OF VETERINARY MEDICAL SCIENCE, Jan. 2016, Peer-reviewed

  
• Structure of the Ebola Virus Nucleocapsid Core by Single Particle Cryo-Electron Microscopy

  Sugita Yukihiko; Kawaoka Yoshihiro; Noda Takeshi; Wolf Matthias

  Microscopy and Microanalysis, 2016, Peer-reviewed, Lead author

  
• Actin-Modulating Protein Cofilin Is Involved in the Formation of Measles Virus Ribonucleoprotein Complex at the Perinuclear Region

  Ritsuko Koga; Yukihiko Sugita; Takeshi Noda; Yusuke Yanagi; Shinji Ohno

  JOURNAL OF VIROLOGY, Oct. 2015, Peer-reviewed

  
• Influenza Virus-Host Interactome Screen as a Platform for Antiviral Drug Development

  Tokiko Watanabe; Eiryo Kawakami; Jason E. Shoemaker; Tiago J. S. Lopes; Yukiko Matsuoka; Yuriko Tomita; Hiroko Kozuka-Hata; Takeo Gorai; Tomoko Kuwahara; Eiji Takeda; Atsushi Nagata; Ryo Takano; Maki Kiso; Makoto Yamashita; Yuko Sakai-Tagawa; Hiroaki Katsura; Naoki Nonaka; Hiroko Fujii; Ken Fujii; Yukihiko Sugita; Takeshi Noda; Hideo Goto; Satoshi Fukuyama; Shinji Watanabe; Gabriele Neumann; Masaaki Oyama; Hiroaki Kitano; Yoshihiro Kawaoka

  CELL HOST & MICROBE, Dec. 2014, Peer-reviewed

  
• Configuration of Viral Ribonucleoprotein Complexes within the Influenza A Virion

  Yukihiko Sugita; Hiroshi Sagara; Takeshi Noda; Yoshihiro Kawaoka

  JOURNAL OF VIROLOGY, Dec. 2013, Peer-reviewed, Lead author

  
• Characterization of H7N9 influenza A viruses isolated from humans

  Tokiko Watanabe; Maki Kiso; Satoshi Fukuyama; Noriko Nakajima; Masaki Imai; Shinya Yamada; Shin Murakami; Seiya Yamayoshi; Kiyoko Iwatsuki-Horimoto; Yoshihiro Sakoda; Emi Takashita; Ryan McBride; Takeshi Noda; Masato Hatta; Hirotaka Imai; Dongming Zhao; Noriko Kishida; Masayuki Shirakura; Robert P. de Vries; Shintaro Shichinohe; Masatoshi Okamatsu; Tomokazu Tamura; Yuriko Tomita; Naomi Fujimoto; Kazue Goto; Hiroaki Katsura; Eiryo Kawakami; Izumi Ishikawa; Shinji Watanabe; Mutsumi Ito; Yuko Sakai-Tagawa; Yukihiko Sugita; Ryuta Uraki; Reina Yamaji; Amie J. Eisfeld; Gongxun Zhong; Shufang Fan; Jihui Ping; Eileen A. Maher; Anthony Hanson; Yuko Uchida; Takehiko Saito; Makoto Ozawa; Gabriele Neumann; Hiroshi Kida; Takato Odagiri; James C. Paulson; Hideki Hasegawa; Masato Tashiro; Yoshihiro Kawaoka

  NATURE, Sep. 2013, Peer-reviewed

  
• Three-dimensional analysis of ribonucleoprotein complexes in influenza A virus

  Takeshi Noda; Yukihiko Sugita; Kazuhiro Aoyama; Ai Hirase; Eiryo Kawakami; Atsuo Miyazawa; Hiroshi Sagara; Yoshihiro Kawaoka

  NATURE COMMUNICATIONS, Jan. 2012, Peer-reviewed

  
• Ultracentrifugation deforms unfixed influenza A virions

  Yukihiko Sugita; Takeshi Noda; Hiroshi Sagara; Yoshihiro Kawaoka

  JOURNAL OF GENERAL VIROLOGY, Nov. 2011, Peer-reviewed, Lead author

  

Misc.

• アフターコロナを生きる高分子 新興ウイルスの構造研究

  杉田征彦

  高分子, 13 Jul. 2023

  
• Architecture of the Heterochromatin Unit Revealed by Cryo-EM

  Takizawa Yoshimasa; Machida Shinichi; Ishimaru Masakazu; Sekine Satoshi; Sugita Yukihiko; Nakayama Jun-ichi; Kurumizaka Hitoshi; Wolf Matthias

  BIOPHYSICAL JOURNAL, Feb. 2018, Peer-reviewed

  
• エボラウイルスのクライオ電子顕微鏡解析

  杉田 征彦

  感染・炎症・免疫, Oct. 2020

  
• Electron Microscopic Analyses on Pathogenic RNA Viruses

  Sugita Yukihiko

  KENBIKYO, 2020

  
• [Structural studies on negative-strand RNA virus].

  Yukihiko Sugita

  Uirusu, 2020

  
• Ebola virus

  杉田 征彦

  ウイルス = Virus, Dec. 2019, Invited

  

Presentations

• エボラウイルスヌクレオカプシドの構造機能解析により明らかになったVP24のウイルス増殖における役割

  藤春(藤田)陽子; 藤春(藤田)陽子; 藤春(藤田)陽子; 胡上帆; 胡上帆; 胡上帆; 平林愛; 平林愛; 高松由基; グ イエニ; グ イエニ; 祝部和也; 祝部和也; 村本裕紀子; 村本裕紀子; 中野雅博; 中野雅博; 杉田征彦; 杉田征彦; 杉田征彦; 野田岳志; 野田岳志

  日本分子生物学会年会プログラム・要旨集(Web), 2024

  
• 広域中和抗体誘導効率向上を目標とするCOVID-19ワクチン抗原設計指針の確立

  逸見拓矢; 城田凛; 石川青空; 石川青空; 杉田征彦; 杉田征彦; 矢島久乃; 王一寧; 佐々木慈英; 相内章; 相内章; 野田岳志; 鈴木忠樹; 橋口隆生

  日本ワクチン学会学術集会プログラム・抄録集, 2024

  
• Establishment of universal corona vaccine antigen design guidelines

  逸見拓矢; 城田凜; 石川青空; 石川青空; 杉田征彦; 杉田征彦; 矢島久乃; 相内章; 相内章; 野田岳志; 鈴木忠樹; 橋口隆生

  日本ウイルス学会学術集会プログラム・予稿集(Web), 2024

  
• Molecular basis of inhibition of type II interferon pathway by rabies virus P-protein

  南未来; 武川祐一郎; 杉山葵; 飯塚祐太; 稲葉理美; MOSELEY Gregory W.; 杉田征彦; 尾瀬農之

  日本ウイルス学会学術集会プログラム・予稿集(Web), 2024

  
• クライオ電子顕微鏡を用いた,狂犬病ウイルスによるヒトSTAT1経路阻害の構造基盤

  南未来; 杉山葵; 武川祐一郎; 姚閔; 姚閔; 稲葉理美; 稲葉理美; 廣瀬未果; 杉田征彦; 尾瀬農之; 尾瀬農之

  日本蛋白質科学会年会(Web), 2024

  
• Search for respiratory syncytial virus therapeutic targets through phenotypic screening from drug repurposing in pediatric drugs

  小笠原徳子; 小笠原徳子; 山本聡; 谷向由佳; 吉田有梨枝; 杉田征彦; 佐藤彰彦; 佐藤彰彦; 澤洋文; 大場靖子; 勝山彬; 勝山彬; 勝山彬; 市川聡; 市川聡; 市川聡; 吉田圭太朗; 高野賢一; 横田伸一

  日本ウイルス学会学術集会プログラム・予稿集(Web), 2024

  
• 狂犬病ウイルスP蛋白質によるSTAT阻害機構解明のための,複合体クライオ電顕構造解析に向けた取り組み

  杉山葵; 南未来; 杉田征彦; 廣瀬未果; 喜多俊介; 喜多俊介; 有森貴夫; 高木淳一; 前仲勝実; 前仲勝実; 姚閔; 姚閔; 尾瀬農之; 尾瀬農之

  日本蛋白質科学会年会(Web), 2024

  
• Insights into the structure of the Lloviu virus nucleoprotein-RNA complex through cryo-EM

  HU Shangfan; FUJITA-FUJIHARU Yoko; SUGITA Yukihiko; WENDT Lisa; MURAMOTO Yukiko; NAKANO Masahiro; HOENEN Thomas; NODA Takeshi

  日本ウイルス学会学術集会プログラム・予稿集(Web), 26 Sep. 2023

  
• Structural insights into human kappa opioid receptor signaling by biased ligand

  SUNO Chiyo; SUNO Ryoji; NISHIKAWA Ryo; SUZUKI Riko; IWATA Seiya; TAKAI Tomoyo; OGURA Takaya; HIROSE Mika; INOUE Akitoshi; KISE Ryoji; SUGITA Yukihiko; SAITO Tsuyoshi; KATAYAMA Kota; INOUE Asuka; KATO Takayuki; NAGASE Hiroshi; KANDORI Hideki; KOBAYASHI Takuya

  日本分子生物学会年会プログラム・要旨集(Web), 2023

  
• Significance of the “rule of 6” for the genome replication of negative-strand RNA viruses

  MATSUMOTO Yusuke; KAWASAKI Junna; SUGITA Yukihiko; URATA Shuzo

  日本RNA学会年会要旨集, 2023

  
• SARS-CoV-2始祖株とオミクロンBA.5の比較による有用ワクチン抗原設計指針の探索

  逸見 拓矢; 石川 青空; 矢島 久乃; 佐々木 慈英; 杉田 征彦; 相内 章; 野田 岳志; 鈴木 忠樹; 橋口 隆生

  臨床とウイルス, Sep. 2023, 日本臨床ウイルス学会

  
• Three-dimensional structures of the mononegavirus nucleoprotein-RNA supermolecular complex

  Yukihiko Sugita

  日本分子生物学会年会プログラム・要旨集(Web), 08 Dec. 2023, Invited

  
• 狂犬病ウイルスPタンパク質によるSTAT阻害機構の解明

  南未来; 杉山葵; 武川祐一郎; 姚閔; 姚閔; 廣瀬未果; 杉田征彦; 尾瀬農之; 尾瀬農之

  日本蛋白質科学会年会プログラム・要旨集, 2023

  
• ボルナ病ウイルス核タンパク質-RNA複合体形成の構造基盤

  後藤真也; 平井悠哉; 朝長啓造; 野田岳志; 堀江真行; 杉田征彦; 杉田征彦

  日本蛋白質科学会年会プログラム・要旨集, 05 Jul. 2023

  
• Structural insights into the Borna disease virus 1 nucleoprotein-RNA complex assembly

  後藤真也; 平井悠哉; 朝長啓造; 野田岳志; 堀江真行; 杉田征彦

  日本ウイルス学会学術集会プログラム・予稿集(Web), 28 Sep. 2023

  
• Three-dimensional structures of the Borna disease virus 1 nucleoprotein-RNA complex

  杉田征彦; 杉田征彦; 杉田征彦; 平井悠哉; 後藤真也; 後藤真也; 朝長啓造; 朝長啓造; 朝長啓造; 野田岳志; 野田岳志; 堀江真行; 堀江真行

  日本ウイルス学会学術集会プログラム・予稿集(Web), 26 Sep. 2023

  
• クライオ電子顕微鏡から見えてきたウェルシュ菌イオタ毒素の膜孔形成機構

  山田等仁; 杉田征彦; 杉田征彦; 野田岳志; 津下英明

  トキシンシンポジウム予稿集, 2023

  
• 電子顕微鏡でウイルスをみる

  杉田征彦

  トキシンシンポジウム予稿集, 06 Sep. 2023, Invited

  
• Neutralizing mechanism of Influenza virus mediated by multimeric IgA

  山内康司; 藤田陽子; 平林愛; 杉田征彦; 齊藤慎二; 鈴木忠樹; 中野雅博; 村本裕紀子; 野田岳志

  日本ウイルス学会学術集会プログラム・予稿集(Web), 26 Sep. 2023

  
• Exploring useful vaccine antigens using spike proteins with different structures

  逸見拓矢; 逸見拓矢; 石川青空; 石川青空; 矢島久乃; 佐々木慈英; 杉田征彦; 杉田征彦; 相内章; 相内章; 野田岳志; 鈴木忠樹; 橋口隆生

  日本ウイルス学会学術集会プログラム・予稿集(Web), 2023

  
• Structural studies of;pathogenic RNA viruses using electron microscopy

  Yukihiko Sugita

  Innovation seminar, Graduate School of Life sciences, Tohoku University, 13 Mar. 2023, Takeshi Yokoyama, Invited

  
• Cryo-EM structure of the nucleoprotein-RNA complex of a novel filovirus, Lloviu virus

  Shangfan Hu; Yoko Fujita-Fujiharu; Yukihiko Sugita; Lisa Wend; Yukiko Muramoto; Masahiro Nakano; Thomas Hoenen; Takeshi Noda

  The 60th Annual Meeting of the Biophysical Society of Japan, 29 Sep. 2022

  
• マイナス鎖RNAウイルスの核タンパク質−RNA複合体構造

  杉田 征彦

  日本顕微鏡学会第65回シンポジウム, 05 Nov. 2022, Invited

  
• 単粒子解析法による螺旋構造解析

  杉田征彦

  日本蛋白質科学会年会プログラム・要旨集, 2021, Invited

  
• ヒトプロスタグランジンE2受容体EP3-Gタンパク質複合体のクライオ電子顕微鏡単粒子解析

  寿野良二; 杉田征彦; 森本和志; 辻本浩一; 廣瀬未果; 寿野千代; 野村紀通; 岩崎憲二; 加藤貴之; 岩田想; 小林拓也

  日本蛋白質科学会年会プログラム・要旨集, 2021

  
• Structural studies on negative-strand RNA viruses using cryo-electron microscopy

  Yukihiko Sugita

  日本分子生物学会年会プログラム・要旨集(Web), 2020, Invited

  
• Structure of the Ebola virus core by single-particle cryo-EM

  Yukihiko Sugita

  International Symposium on Diffraction Structure Biology 2019, 19 Oct. 2019, Invited

  
• 病原体の生化学-基礎感染症学から創薬開発まで- クライオ電子顕微鏡法を用いたエボラウイルス・核タンパク質-RNA複合体の構造解析

  杉田 征彦; 松波 秀行; 河岡 義裕; 野田 岳志; ウォルフ・マティアス

  日本生化学会大会プログラム・講演要旨集, Sep. 2019, (公社)日本生化学会, Invited

  
• Cryo‐EM structure of Ebola virus nucleoprotein‐RNA complex

  SUGITA Yukihiko; MATSUNAMI Hideyuki; KAWAOKA Yoshihiro; NODA Takeshi; WOLF Matthias

  日本細胞生物学会大会(Web), 25 Jun. 2019, Invited

  
• Ebola Virus - Core structure of the Ebola virus -

  Yukihiko Sugita

  Fondation Pasteur Japon Seminar Series 1 "Structure and Functions of Virus", 14 Jun. 2019, Invited

  
• エボラウイルス・コア構造のクライオ電子顕微鏡解析

  杉田 征彦

  創薬産業構造解析コンソーシアム 分野別研究会, 15 Mar. 2019, Invited

  
• クライオ電子顕微鏡による原子分解能解析

  岩崎憲治; 杉田征彦; 廣瀬未果; 川本晃大; 宮崎直幸

  日本農芸化学会大会講演要旨集(Web), 05 Mar. 2019, Invited

  
• ウイルス蛋白質‐核酸複合体サンプルの調製と解析

  杉田征彦

  KEK Proceedings (Web), Feb. 2019

  
• エボラウイルスのNP-RNA複合体とヌクレオカプシドの形成に重要なアミノ酸残基の同定

  祝部和也; 藤田陽子; 杉田征彦; 村本裕紀子; 胡上帆; 宮本翔; 中野雅博; WOLF Matthias; 野田岳志

  日本ウイルス学会学術集会プログラム・予稿集(Web), 2019

  
• Cryo-EM structure of a flexible helical complex - The core structure of the Ebola virus-

  Yukihiko Sugita

  NIPS EM Workshop 2018, 14 Nov. 2018

  
• Cryo-electron microscopic structure of the Ebola virus nucleoprotein-RNA complex at near-atomic resolution

  SUGITA Yukihiko; MATSUNAMI Hideyuki; KAWAOKA Yoshihiro; KAWAOKA Yoshihiro; NODA Takeshi; WOLF Matthias

  日本ウイルス学会学術集会プログラム・抄録集, 18 Sep. 2018

  
• Purification and Analysis on viral protein-RNA complexes

  Yukihiko Sugita

  Photon Factroy Workshop, 08 Sep. 2018, Invited

  
• Structure of Ebola virus nucleoprotein-RNA complex by single-particle cryo-electron microscopy

  SUGITA Yukihiko; MATSUNAMI Hideyuki; KAWAOKA Yoshihiro; KAWAOKA Yoshihiro; NODA Takeshi; WOLF Matthias

  The 56th Annual Meeting of the Biophysical Society of Japan. China-Japan Joint Symposium: Cryo electron microscopy, 20 Aug. 2018, Invited

  
• 最先端クライオ電子顕微鏡による近原子分解能解析の支援提供

  廣瀬未果; 宮崎直幸; 川本晃大; 杉田征彦; 常住規代; 岩崎憲治

  日本蛋白質科学会年会プログラム・要旨集, 23 May 2018

  
• Architecture of the Heterochromatin Unit Revealed by Cryo-EM

  Takizawa Yoshimasa; Machida Shinichi; Ishimaru Masakazu; Sekine Satoshi; Sugita Yukihiko; Nakayama Jun-ichi; Kurumizaka Hitoshi; Wolf Matthias

  BIOPHYSICAL JOURNAL, Feb. 2018

  
• Identification of lung-derived exosomal microRNAs that regulate the innate immune response to influenza virus infection

  MAEMURA Tadashi; FUKUYAMA Satoshi; LOPES Tiago J. S; SUGITA Yukihiko; NODA Takeshi; KAWAOKA Yoshihiro; KAWAOKA Yoshihiro

  日本ウイルス学会学術集会プログラム・抄録集, 30 Sep. 2016

  
• Cryo-electron microscopic analysis of influenza virus ribonucleoprotein complex during transcription

  Sugita Y; Nakano M; Shindo K; Muramoto Y; Kodera N; Kawaoka Y; Noda T; Wolf M

  East Asia Joint Symposium 2015, 15 Nov. 2015

  
• cryo-electron tomographic analysis of influenza viral transcription

  Sugita Y; Nakano M; Shindo K; Muramoto Y; Kodera N; Kawaoka Y; Wolf M; Noda T

  Gordon Research Conference Three Dimensional Electron Microscopy, 14 Jun. 2015

  
• インフルエンザウイルス感染マウスの肺由来エクソソームの同定

  前村忠; 福山聡; 杉田征彦; 河岡義裕

  日本獣医学会学術集会講演要旨集, 11 Aug. 2014

  
• Morphogenesis of influenza virus particle, Invited Seminar of Okinawa Institute of Science and Technology

  Yukihiko Sugita

  Invited Seminar of Okinawa Institute of Science and Technology, 08 Jan. 2014, Invited

  
• インフルエンザウイルスRNPのウイルス粒子内への取込み方向性の解析

  杉田征彦; 相良洋; 野田岳志; 河岡義裕

  日本ウイルス学会学術集会プログラム・抄録集, 31 Oct. 2012

  
• 近年北海道で分離された牛ウイルス性下痢ウイルスの遺伝子と抗原性の解析

  迫田義博; 田村友和; 杉田征彦; 吉野史; 岡松正敏; 喜田宏

  日本ウイルス学会学術集会プログラム・抄録集, 15 Oct. 2010

  
• 豚コレラウイルスの病原性解析に用いる感染性クローンの作出

  吉野史; 迫田義博; 野村拓志; 山本直樹; 杉田征彦; 岡松正敏; RUGGLI Nicolas; 喜田宏

  日本ウイルス学会学術集会プログラム・抄録集, 15 Oct. 2010

  
• 牛ウイルス性下痢ウイルス2b亜型(BVDV‐2b)株の性状

  田村友和; 迫田義博; 吉野史; 杉田征彦; 岡松正敏; 長井誠; 伊藤美加; 青木博史; 喜田宏

  日本ウイルス学会学術集会プログラム・抄録集, 15 Oct. 2010

  
• 近年北海道で分離された牛ウイルス性下痢ウイルスの遺伝子解析

  田村友和; 迫田義博; 杉田征彦; 吉野史; 岡松正敏; 喜田宏

  日本獣医学会学術集会講演要旨集, 2010

  
• 牛ウイルス性下痢ウイルス(BVDV)の表面糖蛋白E2に対するモノクローン抗体の作出と抗原性解析

  杉田征彦; 迫田義博; 吉田裕美; 岡松正敏; 喜田宏

  日本獣医学会学術集会講演要旨集, 2010

  
• 豚コレラ弱毒生ワクチン株の豚扁桃継代による病原性の復帰

  吉野史; 迫田義博; 杉田征彦; 野村拓志; 山本直樹; 岡松正敏; 喜田宏

  日本獣医学会学術集会講演要旨集, 2010

  
• 豚コレラウイルスの病原性の分子基盤―弱毒生ワクチン株の豚継代による病原性の部分復帰―

  迫田義博; 岡松正敏; 野村拓志; 杉田征彦; 吉野史; 喜田宏

  日本ウイルス学会学術集会プログラム・抄録集, 01 Oct. 2009

  
• バキュロウイルスの特性を利用した鳥インフルエンザワクチンの開発

  栗林沙弥; 迫田義博; 杉田征彦; 城本麻衣; 城本麻衣; 津田祥美; 岡松正敏; 松浦善治; 喜田宏

  日本ウイルス学会北海道支部夏季シンポジウム講演抄録, 2008

  
• Introduction of the cryo-EM facility at Institute for Frontier Life and Medical Sciences, Kyoto University

  Sugita Y; Noda T

  日本分子生物学会年会プログラム・要旨集(Web), 2021, Invited

  

Awards

• Oct. 2019

  日本ウイルス学会, 杉浦奨励賞
• Nov. 2019

  大阪大学, 大阪大学賞（若手教員部門）
• Mar. 2020

  風戸研究奨励会, 風戸研究奨励賞

Media Coverage

• The excitement of "seeing the structure" is the driving force behind the exploration.

  Preducts Inc., Preducts Journal, Dec. 2023, Internet

  
• 関西医科大、発熱、炎症などに関与するプロスタグランジン受容体EP3 シグナリング複合体の可視化～緑内障、高眼圧症治療薬の合理的設計に貢献～

  Nikkei Business Publications,Inc., Nikkei Business Publications, 14 Sep. 2022, Internet

  
• 創発的研究支援事業の研究者８人 末松文科相と車座対話

  科学新聞, 科学新聞, 15 Apr. 2022, Paper

  
• エボラ中心部 内部構造解明 沖縄科技大学院大など

  Newton 1月号, 7ページ, 26 Nov. 2018, Paper

  
• 世界を震撼させたエボラ出血熱 – 「見えない敵」との闘いに挑む

  朝日新聞ＧＬＯＢＥ＋, 26 Oct. 2018, Internet

  
• エボラ中心部 内部構造解明 沖縄科技大学院大など

  東京読売新聞, 3ページ, 22 Oct. 2018, Paper

  
• エボラの構造を解明-OISTなど 薬品開発に期待

  琉球新報, 28面社会, 18 Oct. 2018, Paper

  
• エボラウイルス 構造解明 OISTなど共同研究チーム

  沖縄タイムス, 29面社会, 18 Oct. 2018, Paper

  
• 世界初-エボラウイルスの基本構造解明

  日本テレビ, 日テレNEWS24, 18 Oct. 2018, Media report

  
• エボラウイルスの構造解明＝治療法開発に貢献期待ー沖縄科技大など

  時事通信, 18 Oct. 2018, Paper

  
• エボラ出血熱の治療薬へ道 沖縄科技大、ウイルス構造解明

  日刊工業新聞, 18 Oct. 2018, Paper

  
• エボラウイルス構造解明 沖縄科技大など 治療薬開発に道

  日本経済新聞, 34面, 18 Oct. 2018, Paper

  

External funds: Kakenhi

• Structural basis of the filovirus assembly and replication

  Grant-in-Aid for Scientific Research (C)

  Basic Section 49060:Virology-related

  Kyoto University

  杉田 征彦; 杉田征彦

  From 01 Apr. 2021, To 31 Mar. 2024, Granted

  フィロウイルス;クエヴァウイルス;エボラウイルス;マールブルグウイルス;クライオ電子顕微鏡;ヌクレオカプシド

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K07052/
• Molecular basis of counteraction against the JAK-STAT signaling pathway by virus

  Grant-in-Aid for Scientific Research (B)

  Basic Section 43020:Structural biochemistry-related

  Hokkaido University

  尾瀬 農之

  From 01 Apr. 2021, To 31 Mar. 2024, Granted

  複合体解析;相互作用解析;NMR;クライオ電顕;結晶解析;シグナル伝達阻害;ウイルス蛋白質;クライオ電子顕微鏡

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H02408/
• Structural basis for the formation of influenza virus ribonucleoprotein complex

  Grant-in-Aid for Early-Career Scientists

  Basic Section 49060:Virology-related

  Kyoto University

  Yukihiko Sugita

  From 01 Apr. 2019, To 31 Mar. 2021, Project Closed

  インフルエンザウイルス;微細構造;電子顕微鏡;クライオ電子顕微鏡法;クライオ電子顕微鏡

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K16667/
• Assembly mechanism of the influenza virus genome

  Grant-in-Aid for Young Scientists (B)

  Osaka University

  Yukihiko SUGITA

  From 01 Apr. 2017, To 31 Mar. 2019, Project Closed

  ウイルス;電子顕微鏡;インフルエンザウイルス

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K15704/
• Mechanism of Ebola viral transcription and replication

  Grant-in-Aid for Research Activity Start-up

  Okinawa Institute of Science and Technology Graduate University

  Sugita Yukihiko

  From 29 Aug. 2014, To 31 Mar. 2016, Project Closed

  エボラウイルス;クライオ電子顕微鏡;ウイルス学;電子顕微鏡

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26892028/
• Design of highly ordered protein assemblies with mesoscale conformational change ability

  Grant-in-Aid for Transformative Research Areas (B)

  Transformative Research Areas, Section (II)

  Kyoto University

  鈴木 雄太

  From 23 Aug. 2021, To 31 Mar. 2024, Granted

  タンパク質デザイン;タンパク質工学;バイオマテリアル;タンパク質集合体

  https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-21H05117/
• Mechanism of mononegavirus nucleocapsid formation

  Grant-in-Aid for Scientific Research (B)

  Basic Section 49060:Virology-related

  Kyoto University

  杉田 征彦

  From 01 Apr. 2024, To 31 Mar. 2027, Granted

  RNAウイルス;モノネガウイルス;ヌクレオカプシド;クライオ電子顕微鏡

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K02284/
• Toxin membrane translocation system approached by liposome single particle analysis

  Grant-in-Aid for Scientific Research (B)

  Basic Section 43040:Biophysics-related

  Kyoto Sangyo University

  津下 英明

  From 01 Apr. 2024, To 31 Mar. 2027, Granted

  トキシン膜透過システム;二成分毒素;タンパク質膜透過;クライオ電子顕微鏡;１分子測定

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K01993/
• 出力多様性を司るSTAT多量体化の構造基盤と，それに抗するウイルス蛋白質の解析

  Grant-in-Aid for Scientific Research (B)

  Basic Section 43020:Structural biochemistry-related

  Hokkaido University

  尾瀬 農之

  From 01 Apr. 2024, To 31 Mar. 2028, Granted

  JAK-STAT;免疫逃避;クライオ電顕;NMR

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K01959/
• A Preliminary Cross-disciplinary Study of Traditional Medicine on Zoonotic diseases in Indo-Tibetan cultures

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 1:Philosophy, art, and related fields

  Koyasan University

  菊谷 竜太

  From 28 Jun. 2024, To 31 Mar. 2027, Granted

  インド・チベット仏教文化圏;人畜共通感染症;伝統医学;ウイルス学;生物多様性

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K21354/

External funds: others

• やわらかな病原性エンベロープウイルスの構造解明

  国立研究開発法人科学技術振興機構, 創発的研究支援事業

  From 01 Apr. 2022, To 31 Mar. 2029

  杉田 征彦
• クライオ電子顕微鏡によるインフルエンザウイルス・リボ核タンパク質複合体の構造解析

  武田科学振興財団, ライフサイエンス研究助成

  From 01 Nov. 2019, To 01 Apr. 2021

  杉田　征彦

Education

Teaching subject(s)

• From 01 Apr. 2024, To 31 Mar. 2025

  Introduction to Biology and Life Science

  N484, Spring, Institute for Liberal Arts and Sciences, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Course in Immunology and Human Disorders

  1031, Fall, Graduate School of Biostudies, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course and Seminar A

  2006, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course and Seminar B

  2007, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course and Seminar B

  2007, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course and Seminar A

  2006, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Experiments in Integrated Life Sciences

  5012, Spring, Graduate School of Biostudies, 4
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Experiments in Integrated Life Sciences

  5012, Fall, Graduate School of Biostudies, 4
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course and Seminar C

  2008, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course and Seminar D

  2009, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course and Seminar D

  2009, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course and Seminar C

  2008, Spring, Graduate School of Biostudies, 5

Other educational achievements

• ILAS Seminar :Come in touch with Life and Medical Sciences
  Institute for Life and Medical Sciences, Kyoto University
• 新興再興感染症制御学特別講義
  岐阜大学・鳥取大学共同獣医学科
  2022年12月
• ILAS Seminar :The Front Line of Virology and Immunology
  Institute for Liberal Arts and Sciences, Kyoto University

Administration

Faculty management (title, position)

• From 20 Jul. 2023

  学術委員会　委員

Academic, Social Contribution

Academic Contribution

• [Invited Instructor] 2024 Cryo-Electron Microscopy Course

  Other

  Kazutoshi Tani, Bruno M. Humbel, Okinawa Institute of Science and Technology, From 05 Feb. 2024, To 09 Feb. 2024

  
• [Invited Instructor] 2023 Cryo-Electron Microscopy Course at OIST

  Other

  Bruno M. Humbel, Kazutoshi Tani, Okinawa Institute of Science and Technology, From 06 Feb. 2023, To 10 Feb. 2023

  
• [Invited Instructor] FY2020 Cryo-Electron Microscopy Course

  Other

  Bruno M. Humbel, Okinawa Institute of Science and Technology, Okinawa Institute of Science and Technology, From 15 Feb. 2021, To 19 Feb. 2021

  
• [Invited Instructor] FY2019 Cryo-Electron Microscopy Course

  Other

  Bruno M. Humbel, Okinawa Institute of Science and Technology, Okinawa Institute of Science and Technology, From 03 Feb. 2020, To 07 Feb. 2020

  
• [Invited Instructor] Cryo-Electron Microscopy Course

  Other

  Bruno M. Humbel, Okinawa Institute of Science and Technology, Okinawa Institute of Science and Technology, From 18 Feb. 2019, To 22 Feb. 2019

  
• [Invited Instructor] FY2021 Cryo-Electron Microscopy Course

  Other

  Bruno M. Humbel, Okinawa Institute of Science and Technology, From 28 Feb. 2022, To 04 Mar. 2022