Basic Information

Faculty

• Faculty of Science

Email Address

• Email Address

  goto.yuhei.4ckyoto-u.ac.jp

Professional Memberships

• THE MOLECULAR BIOLOGY SOCIETY OF JAPAN
• 酵母遺伝学フォーラム
• JAPAN SOCIETY FOR CELL BIOLOGY
• 日本農芸化学会

Academic Degree

• 25 Mar. 2013

  東京大学修士（理学）
• 24 Apr. 2017

  東京大学博士（理学）

Academic Resume (Undergraduate School/Majors)

• 東京大学, 理学部生物化学科, 卒業

Research History

• From Jun. 2024, To Present

  京都大学, 生命科学研究科, 准教授
• From Nov. 2019, To May 2024

  National Institute for Basic Biology, Division of Quantitative Biology, Research Associate
• From Apr. 2017, To Oct. 2019

  National Institute for Basic Biology, Division of quantitative biology, Post-Doc
• From Apr. 2015, To Mar. 2017

  Japan Society for the Promotion of Science, 特別研究員 DC2

Profile

• Profile

  細胞が外界からの刺激や自分自身の状態からどのような表現型を選択していくのかに興味を持っています。

  
  

  分裂酵母やヒト培養細胞を材料とし、蛍光プローブをもとにした定量イメージングから細胞運命決定にかかわる分子メカニズムを見出し、また、それを光遺伝学などを用いて構成的に理解することを目指しています。

  
  

  直近の興味は、細胞周期停止からの再開の運命決定機構、細胞周期の長さの決定機構やその進化についてです。

ID,URL

• J-Global ID

  201701007885674335

researchmap URL

• https://researchmap.jp/yuheigoto

Research

Research Interests

• Quantitative Biology
• 蛍光バイオセンサー
• Optogenetics
• Cell cycle
• 細胞休眠
• 細胞質物性
• Fission yeast
• Phytochrome
• FCS/FCCS
• CRISPR/Cas9 Knock-in

Research Areas

• Life sciences, Cell biology, Development of Optogenetics tool
• Life sciences, Cell biology, single-cell quantitative imaging

Papers

• Opto-p53: A Light-Controllable p53 Signaling for Regulating p53-Dependent Cell Fate.

  Tatsuki Tsuruoka; Yuhei Goto; Kazuhiro Aoki

  bioRxiv, 06 Apr. 2025

  
• Network topology creates independent control of G2-M from G1-S checkpoints in the fission yeast cell cycle system

  Yuhei Yamauchi; Hironori Sugiyama; Yuhei Goto; Kazuhiro Aoki; Atsushi Mochizuki

  bioRxiv, 13 Mar. 2025

  
• Possible roles of CAHS proteins from Tardigrade in osmotic stress tolerance in mammalian cells

  Takahiro Bino; Yuhei Goto; Gembu Maryu; Kazuharu Arakawa; Kazuhiro Aoki

  Cell Structure and Function, Nov. 2024, Peer-reviewed

  
• Quantification of Cyclin-CDK dissociation constants in living cells using fluorescence cross-correlation spectroscopy with green and near-infrared fluorescent proteins.

  Aika Toyama; Yuhei Goto; Yuhei Yamauchi; Hironori Sugiyama; Yohei Kondo; Atsushi Mochizuki; Kazuhiro Aoki

  bioRxiv, 15 Nov. 2024, Corresponding author

  
• Cytoplasmic fluidization contributes breaking spore dormancy in fission yeast.

  Keiichiro Sakai; Yohei Kondo; Yuhei Goto; Kazuhiro Aoki

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 18 Jun. 2024, Peer-reviewed, Corresponding author

  Access this paper :

  
• Live-cell fluorescence imaging and optogenetic control of PKA kinase activity in fission yeastSchizosaccharomyces pombe.

  Keiichiro Sakai; Kazuhiro Aoki; Yuhei Goto

  Yeast, 07 Apr. 2024, Peer-reviewed, Last author, Corresponding author

  
• Live-cell imaging defines a threshold in CDK activity at the G2/M transition

  Hironori Sugiyama; Yuhei Goto; Yohei Kondo; Damien Coudreuse; Kazuhiro Aoki

  Developmental Cell, 15 Jan. 2024, Peer-reviewed, Lead author

  
• Cellular responses to compound stress induced by atmospheric-pressure plasma in fission yeast

  Yoko Otsubo; Akira Yamashita; Yuhei Goto; Keiichiro Sakai; Tetsushi Iida; Shinji Yoshimura; Katsuki Johzuka

  Journal of Cell Science, 22 Nov. 2023, Peer-reviewed

  
• Chemogenetic Manipulation of Endogenous Proteins in Fission Yeast Using a Self-Localizing Ligand-Induced Protein Translocation System.

  Akinobu Nakamura; Yuhei Goto; Hironori Sugiyama; Shinya Tsukiji; Kazuhiro Aoki

  ACS chemical biology, 22 Nov. 2023, Peer-reviewed, Corresponding author

  
• Quantitative live-cell imaging of GPCR downstream signaling dynamics

  Ryosuke Tany; Yuhei Goto; Yohei Kondo; Kazuhiro Aoki

  Biochemical Journal, 29 Apr. 2022, Peer-reviewed, Corresponding author

  
• Near-infrared imaging in fission yeast using a genetically encoded phycocyanobilin biosynthesis system

  Keiichiro Sakai; Yohei Kondo; Hiroyoshi Fujioka; Mako Kamiya; Kazuhiro Aoki; Yuhei Goto

  Journal of Cell Science, 15 Dec. 2021, Peer-reviewed, Last author, Corresponding author

  
• A novel red fluorescence dopamine biosensor selectively detects dopamine in the presence of norepinephrine in vitro

  Chihiro Nakamoto; Yuhei Goto; Yoko Tomizawa; Yuko Fukata; Masaki Fukata; Kasper Harpsøe; David E. Gloriam; Kazuhiro Aoki; Tomonori Takeuchi

  Molecular Brain, Dec. 2021, Peer-reviewed, Lead author

  
• Identification of ksg1 mutation showing long‐lived phenotype in fission yeast

  Kotaro Matsui; Keisuke Okamoto; Tomoka Hasegawa; Hokuto Ohtsuka; Takafumi Shimasaki; Kunio Ihara; Yuhei Goto; Kazuhiro Aoki; Hirofumi Aiba

  Genes to Cells, 17 Sep. 2021, Peer-reviewed

  
• A microtubule‐LUZP1 association around tight junction promotes epithelial cell apical constriction

  Tomoki Yano; Kazuto Tsukita; Hatsuho Kanoh; Shogo Nakayama; Hiroka Kashihara; Tomoaki Mizuno; Hiroo Tanaka; Takeshi Matsui; Yuhei Goto; Akira Komatsubara; Kazuhiro Aoki; Ryosuke Takahashi; Atsushi Tamura; Sachiko Tsukita

  The EMBO Journal, 21 Dec. 2020, Peer-reviewed

  
• Improvement of Phycocyanobilin Synthesis for Genetically Encoded Phytochrome-Based Optogenetics

  Youichi Uda; Haruko Miura; Yuhei Goto; Kei Yamamoto; Yusuke Mii; Yohei Kondo; Shinji Takada; Kazuhiro Aoki

  ACS chemical biology, Nov. 2020, Peer-reviewed

  
• Development of optogenetic tools to manipulate cell cycle checkpoints

  Yuhei Goto; Kazuhiro Aoki

  bioRxiv, 23 Jun. 2020, Lead author, Corresponding author

  
• Single-cell quantification of the concentrations and dissociation constants of endogenous proteins.

  Akira T Komatsubara; Yuhei Goto; Yohei Kondo; Michiyuki Matsuda; Kazuhiro Aoki

  The Journal of biological chemistry, 12 Apr. 2019, Peer-reviewed, Lead author

  
• Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling

  Youichi Uda; Yuhei Goto; Shigekazu Oda; Takayuki Kohchi; Michiyuki Matsuda; Kazuhiro Aoki

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Nov. 2017, Peer-reviewed

  
• Pds5 Regulates Sister-Chromatid Cohesion and Chromosome Bi-orientation through a Conserved Protein Interaction Module

  Yuhei Goto; Yuya Yamagishi; Miyuki Shintomi-Kawamura; Mayumi Abe; Yuji Tanno; Yoshinori Watanabe

  CURRENT BIOLOGY, Apr. 2017, Peer-reviewed, Lead author

  
• Crystal structures of the TRIC trimeric intracellular cation channel orthologues

  Go Kasuya; Masahiro Hiraizumi; Andres D. Maturana; Kaoru Kumazaki; Yuichiro Fujiwara; Keihong Liu; Yoshiko Nakada-Nakura; So Iwata; Keisuke Tsukada; Tomotaka Komori; Sotaro Uemura; Yuhei Goto; Takanori Nakane; Mizuki Takemoto; Hideaki E. Kato; Keitaro Yamashita; Miki Wada; Koichi Ito; Ryuichiro Ishitani; Motoyuki Hattori; Osamu Nureki

  CELL RESEARCH, Dec. 2016, Peer-reviewed

  
• Mad1 promotes chromosome congression by anchoring a kinesin motor to the kinetochore

  Takashi Akera; Yuhei Goto; Masamitsu Sato; Masayuki Yamamoto; Yoshinori Watanabe

  NATURE CELL BIOLOGY, Sep. 2015, Peer-reviewed

  
• Kinetochore composition and its function: lessons from yeasts

  Yuya Yamagishi; Takeshi Sakuno; Yuhei Goto; Yoshinori Watanabe

  FEMS Microbiology Reviews, 01 Mar. 2014, Peer-reviewed

  

Misc.

• Molecular and Biophysical Perspectives on Dormancy Breaking: Lessons from Yeast Spore

  Keiichiro Sakai; Yohei Kondo; Kazuhiro Aoki; Yuhei Goto

  Biomolecules, 11 May 2025, Peer-reviewed, Invited, Last author, Corresponding author

  
• Capturing CDKs in Action: Live-Cell Biosensors Pioneer the New Frontiers in Cell Cycle Research

  Sachiya Nakashima; Aika Toyama; Hironori Sugiyama; Kazuhiro Aoki; Yuhei Goto

  Cell Structure and Function, Mar. 2025, Peer-reviewed, Last author, Corresponding author

  
• 分裂酵母の胞子発芽に伴う細胞質流動化の仕組み

  酒井啓一郎; 後藤祐平; 青木一洋

  バイオサイエンスとインダストリー, 10 Jan. 2025, Peer-reviewed, Invited

  
• GPCRリガンドセンサーの開発

  後藤 祐平; 青木 一洋

  月刊「細胞」2023年 4月臨時増刊号 蛍光タンパク質センサー, 31 Mar. 2023, Invited, Lead author

  
• Shedding light on developmental ERK signaling with genetically encoded biosensors

  Akinobu Nakamura; Yuhei Goto; Yohei Kondo; Kazuhiro Aoki

  Development, 15 Sep. 2021, Peer-reviewed, Invited

  
• ライブセルイメージングによる細胞内シグナル伝達の可視化と光操作

  後藤 祐平; 青木 一洋

  顕微鏡, Sep. 2021, Peer-reviewed, Invited, Lead author, Corresponding author

  
• Visualization and Manipulation of Intracellular Signaling.

  Yuhei Goto; Yohei Kondo; Kazuhiro Aoki

  Advances in experimental medicine and biology, 2021, Peer-reviewed, Invited, Lead author

  
• Red/far-red Light-induced Control of Intracellular Signaling in Mammalian Cells

  GOTO Yuhei; AOKI Kazuhiro

  Seibutsu Butsuri, 2019, Lead author

  
• Optogenetic Tools for Quantitative Biology: The Genetically Encoded PhyB–PIF Light-inducible Dimerization System and Its Application for Controlling Signal Transduction

  S Oda; Y Uda; Y Goto; H Miura; K Aoki

  Optogenetics, Sep. 2018, Peer-reviewed, Invited

  
• クローズアップ実験法(series 301) 細胞外からの発色団添加を必要としない赤色光/近赤外光によるシグナル伝達系の光操作

  青木 一洋; 宇田 耀一; 小田 茂和; 後藤 祐平

  実験医学, Aug. 2018

  

Presentations

• CDK活性のライブセルイメージングから酵母の細胞周期を考える

  後藤祐平

  第24回酵母合同シンポジウム, 17 Nov. 2023, Invited

  
• Development of biosensors for measuring TOR and PKA activity to elucidate the nutrition sensing system in fission yeast

  Yuhei Goto; Keiichiro Sakai; Yoshiaki Kamada; Yoko Otsubo; kazuhiro Aoki

  The 61st Annual Meeting of the Biophysical Society of Japan, 15 Nov. 2023, Invited

  
• Development of optogenetic tools to manipulate cell cycle checkpoints

  Yuhei Goto

  3rd Optogenetic Technologies and Applications Conference, 02 Dec. 2022, Invited

  
• Live-Cell Imaging and Optogenetics Reveals AND Gate Role of ERK and Akt in G1/S transition

  Yuhei Goto; Gembu Maryu; Akira T. Komatsubara; Michiyuki Matsuda; Kazuhiro Aoki

  分子生物学会, 04 Dec. 2019, Invited

  

Awards

• 23 Jan. 2025

  第19回わかしゃち奨励賞基礎科学研究部門優秀賞
• 23 Jan. 2025

  第19回わかしゃち奨励賞基礎科学研究部門優秀賞
• 01 Nov. 2019

  地神芳文記念研究助成賞（酵母細胞研究会）
• 25 Mar. 2013

  理学系研究科研究奨励賞（修士）（東京大学）
• 10 Jun. 2020

  若手優秀発表賞（日本細胞生物学会）
• 27 Feb. 2024

  NIBB若手研究者賞（基礎生物学研究所）

Media Coverage

• ニコニコサイエンス生放送「生命の基本単位『細胞』を見て、感じて、操作しよう！〜基礎生物学研究所の最先端技術を見せちゃいます〜」

  ニコニコ生放送, 生命の基本単位『細胞』を見て、感じて、操作しよう！〜基礎生物学研究所の最先端技術を見せちゃいます〜【24時間ニコニコサイエンス】, https://live.nicovideo.jp/watch/lv343110846, 17 Nov. 2023, Internet

  

External funds: Kakenhi

• 固体化した細胞質をもつ休眠細胞からの復帰機構の解明

  Grant-in-Aid for Early-Career Scientists

  Basic Section 44010:Cell biology-related

  National Institute for Basic Biology

  後藤 祐平

  From 01 Apr. 2022, To 31 Mar. 2024, Granted

  分裂酵母;休眠細胞;細胞質固体化;光遺伝学;胞子;定量イメージング

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K15110/
• Analysis of the mechanism underlying G1/S checkpoint threshold by CDK-Cyclin

  Grant-in-Aid for Early-Career Scientists

  Basic Section 43010:Molecular biology-related

  National Institute for Basic Biology

  Yuhei Goto

  From 01 Apr. 2019, To 31 Mar. 2022, Project Closed

  定量イメージング;CDK;サイクリン;分裂酵母;光遺伝学;PhyB;Cry2;FRET;細胞周期;蛍光相関分光法;微小流路;ノックイン;CRISPR-Cas9

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K16050/

Academic, Social Contribution

Committee Memberships

• From Feb. 2021, To Present

  酵母遺伝資源運営委員
• From Oct. 2018, To Mar. 2023

  酵母研究若手の会 運営副委員長
• From Aug. 2017, To Mar. 2023

  運営委員, 酵母研究若手の会
• From Sep. 2022, To Sep. 2022

  酵母遺伝学フォーラム第55回研究報告会 実行委員