Basic Information

Professional Memberships

• Association for Research in Otolaryngology, Regular Member
• THE JAPANESE SOCIETY OF INFLAMMATION AND REGENERATION
• THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE
• THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

Academic Degree

• 24 Mar. 2000

  奈良先端科学技術大学院大学修士（バイオサイエンス）
• 23 Mar. 2005

  京都大学博士（医学）

Academic Resume (Graduate Schools)

• 奈良先端科学技術大学院大学, バイオサイエンス研究科博士前期課程, 修了
• 京都大学, 大学院医学研究科博士課程分子医学系専攻, 研究指導認定退学

Academic Resume (Undergraduate School/Majors)

• 近畿大学, 農学部農芸化学科, 卒業

High School

• High School

  大阪府立　大手前高等学校

Research History

• From Apr. 2004, To Mar. 2007

  Osaka University, Research Institute for Microbial Diseases, 特任研究員
• From Apr. 2007, To Sep. 2008

  産業技術総合研究所, 糖鎖医工学研究センター 分子医用技術開発チーム, 特別研究員
• From Oct. 2008, To Mar. 2012

  産業技術総合研究所, セルエンジニアリング研究部門 組織・再生工学研究グループ, 特別研究員
• From Apr. 2012, To Mar. 2016

  Kyoto University, 耳鼻咽喉科・頭頸部外科, 特定研究員
• From Apr. 2016, To Mar. 2018

  Kyoto University, 耳鼻咽喉科・頭頸部外科, 特定助教
• From Apr. 2018, To Mar. 2022

  Kyoto University, 耳鼻咽喉科・頭頸部外科, 研究員
• From Apr. 2022, To Present

  Kyoto University, Department of Otolaryngology, Head and Neck Surgery, Program Specific Assistant Professor

Language of Instruction

• Japanese

ID,URL

• ORCID ID

  0000-0003-3437-680X

• J-Global ID

  201401070053710782

researchmap URL

• https://researchmap.jp/7000009675

Research

Research Topics, Overview of the research

• Research Topics

  Research on regeneration of otorhinolaryngological tissues using stem cells

• Overview of the research

  Analysis of mechanisms of ENT tissue development, regeneration and aging using molecular and cell biological approaches with stem cells (iPS cells, MSCs, and tissue stem cells).

Research Interests

• nasal epithelium
• larynx
• trachea
• inner ear
• stem cell

Research Areas

• Life sciences, Otorhinolaryngology
• Life sciences, Medical biochemistry, molecular and cellular biology

Papers

• Transplantation of Human Induced Pluripotent Stem Cell-Derived Airway Epithelia at Different Induction Stages into Nude Rat

  Keisuke Mizuno; Hiroe Ohnishi; Yo Kishimoto; Hideaki Okuyama; Yoshitaka Kawai; Masayuki Kitano; Yasuyuki Hayashi; Koichi Omori

  Cellular Reprogramming, 01 Dec. 2024

  
• Identification of stem cell marker-positive subpopulations in the vocal fold of the larynx through transcriptomic analyses

  Keiichi Tamura; Hiroe Ohnishi; Koki Hasebe; Shintaro Fujimura; Tatsuya Katsuno; Zhaonan Zou; Shinya Oki; Yasuyuki Ohkawa; Koichi Omori

  26 Mar. 2025

  
• In vitro model to evaluate effect of acidic pepsin on vocal fold barrier function

  Keisuke Kojima; Tatsuya Katsuno; Yo Kishimoto; Masanobu Mizuta; Ryosuke Nakamura; Hiroe Ohnishi; Koichiro Yamada; Yoshitaka Kawai; Ichiro Tateya; Koichi Omori

  Biochemical and Biophysical Research Communications, Nov. 2024

  
• Rat Tracheal Cartilage Regeneration Using Mesenchymal Stem Cells Derived From Human iPS Cells

  Keisuke Mizuno; Hiroe Ohnishi; Yo Kishimoto; Tsuyoshi Kojima; Shintaro Fujimura; Yoshitaka Kawai; Masayuki Kitano; Makoto Ikeya; Koichi Omori

  Tissue Engineering Part A, 29 Jul. 2024

  
• EBF1 Limits the Numbers of Cochlear Hair and Supporting Cells and Forms the Scala Tympani and Spiral Limbus during Inner Ear Development

  Hiroki Kagoshima; Hiroe Ohnishi; Ryosuke Yamamoto; Akiyoshi Yasumoto; Yosuke Tona; Takayuki Nakagawa; Koichi Omori; Norio Yamamoto

  The Journal of Neuroscience, 04 Jan. 2024, Peer-reviewed

  
• Changes in the Proportion of Each Cell Type After hiPSC-Derived Airway Epithelia Transplantation

  Masayuki Kitano; Yasuyuki Hayashi; Hiroe Ohnishi; Hideaki Okuyama; Masayoshi Yoshimatsu; Keisuke Mizuno; Fumihiko Kuwata; Takeshi Tada; Yo Kishimoto; Satoshi Morita; Koichi Omori

  Cell Transplantation, Jan. 2024, Peer-reviewed, Corresponding author

  
• An infection model for SARS-CoV-2 using rat transplanted with hiPSC-airway epithelial cells.

  Masayuki Kitano; Hiroe Ohnishi; Akiko Makino; Tatsuo Miyamoto; Yasuyuki Hayashi; Keisuke Mizuno; Shinji Kaba; Yoshitaka Kawai; Tsuyoshi Kojima; Yo Kishimoto; Norio Yamamoto; Keizo Tomonaga; Koichi Omori

  Tissue engineering. Part A, 04 Jun. 2024, Peer-reviewed

  
• Comparative study of immunodeficient rat strains in engraftment of hiPSC-derived airway epithelia

  Yasuyuki Hayashi; Hiroe Ohnishi; Masayuki Kitano; Yo Kishimoto; Toshiaki Takezawa; Hideaki Okuyama; Masayoshi Yoshimatsu; Fumihiko Kuwata; Takeshi Tada; Keisuke Mizuno; Koichi Omori

  Tissue Engineering Part A, 11 Nov. 2023, Peer-reviewed

  
• Transplantation of Induced Pluripotent Stem Cell-Derived Airway Epithelia with a Collagen Scaffold into the Nasal Cavity

  Yuji Kitada; Hiroe Ohnishi; Norio Yamamoto; Fumihiko Kuwata; Masayuki Kitano; Keisuke Mizuno; Koichi Omori

  Tissue Engineering Part C: Methods, 01 Nov. 2023, Peer-reviewed

  
• Laryngeal Cartilage Regeneration of Nude Rats by Transplantation of Mesenchymal Stem Cells Derived from Human-Induced Pluripotent Stem Cells

  Keisuke Mizuno; Hiroe Ohnishi; Masayoshi Yoshimatsu; Chengzhu Zhao; Yasuyuki Hayashi; Fumihiko Kuwata; Shinji Kaba; Hideaki Okuyama; Yoshitaka Kawai; Nao Hiwatashi; Yo Kishimoto; Tatsunori Sakamoto; Makoto Ikeya; Koichi Omori

  Cell Transplantation, Jan. 2023, Peer-reviewed

  Access this paper :

  
• Possible application of regenerative medicine to bilateral vestibulopathy

  Taura, A.; Ohnishi, H.; Nishimura, K.; Ogita, H.; Miwa, T.; Ito, J.

  Equilibrium Research, 2021

  
• Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium

  Mami Matsunaga; Ryosuke Yamamoto; Tomoko Kita; Hiroe Ohnishi; Norio Yamamoto; Takayuki Okano; Koichi Omori; Takayuki Nakagawa

  iScience, 17 Feb. 2023, Peer-reviewed

  Access this paper :

  
• Future view of regenerative research for vestibular disorders

  Akiko Taura; Hiroe Ohnishi; Koji Nishimura; Hideaki Ogida; Takayuki Nakagawa; Norio Yamamoto; Takayuki Okano; Koichi Ohmori; Juichi Ito

  Equilibrium Research, 2019

  
• iPS細胞由来気道上皮細胞を用いた中耳粘膜移植を目指す基礎的検討

  多田 剛志; 大西 弘恵; 桑田 文彦; 森野 常太郎; 葛西 善行; 小島 博己; 大森 孝一; 山本 典生

  日本耳鼻咽喉科学会会報, Apr. 2021

  
• Transplantation of a human induced pluripotent stem cell-derived airway epithelial cell sheet into the middle ear of rats

  Takeshi Tada; Hiroe Ohnishi; Norio Yamamoto; Fumihiko Kuwata; Yasuyuki Hayashi; Hideaki Okuyama; Tsunetaro Morino; Yoshiyuki Kasai; Hiromi Kojima; Koichi Omori

  Regenerative Therapy, Mar. 2022, Peer-reviewed

  Access this paper :

  
• Transplantation of human iPS cell-derived airway cells on vitrigel membrane into rat nasal cavity

  Fumihiko Kuwata; Hiroe Ohnishi; Norio Yamamoto; Toshiaki Takezawa; Masaru Yamashita; Hideaki Okuyama; Yasuyuki Hayashi; Masayoshi Yoshimatsu; Yuji Kitada; Takeshi Tada; Masayoshi Kobayashi; Koichi Omori

  Tissue Engineering Part A, 27 Nov. 2021

  
• Insulin-like growth factor 1 promotes the extension of Tracheal Epithelium in an in Vitro Tracheal organ culture model

  Ippei Kishimoto; Hiroe Ohnishi; Kohei Yamahara; Takayuki Nakagawa; Masaru Yamashita; Koichi Omori; Norio Yamamoto

  Auris Nasus Larynx, Jun. 2021, Peer-reviewed

  Access this paper :

  
• iPS細胞由来気道上皮細胞を用いた中耳粘膜移植を目指す基礎的検討

  多田 剛志; 大西 弘恵; 桑田 文彦; 森野 常太郎; 葛西 善行; 小島 博己; 大森 孝一; 山本 典生

  日本耳鼻咽喉科学会会報, Apr. 2021

  
• In vivo regeneration of rat laryngeal cartilage with mesenchymal stem cells derived from human induced pluripotent stem cells via neural crest cells

  Masayoshi Yoshimatsu; Hiroe Ohnishi; Chengzhu Zhao; Yasuyuki Hayashi; Fumihiko Kuwata; Shinji Kaba; Hideaki Okuyama; Yoshitaka Kawai; Nao Hiwatashi; Yo Kishimoto; Tatsunori Sakamoto; Makoto Ikeya; Koichi Omori

  Stem Cell Research, Apr. 2021, Peer-reviewed

  Access this paper :

  
• Semi-automated single-molecule microscopy screening of fast-dissociating specific antibodies directly from hybridoma cultures

  Takushi Miyoshi; Qianli Zhang; Takafumi Miyake; Shin Watanabe; Hiroe Ohnishi; Jiji Chen; Harshad D. Vishwasrao; Oisorjo Chakraborty; Inna A. Belyantseva; Benjamin J. Perrin; Hari Shroff; Thomas B. Friedman; Koichi Omori; Naoki Watanabe

  Cell Reports, Feb. 2021, Peer-reviewed

  Access this paper :

  
• In silico analysis of inner ear development using public whole embryonic body single-cell RNA-sequencing data

  Ryosuke Yamamoto; Hiroe Ohnishi; Koichi Omori; Norio Yamamoto

  Developmental Biology, Jan. 2021, Peer-reviewed

  Access this paper :

  
• Activation of PKC induces leukocyte adhesion by the dephosphorylation of ERM

  Tachibana, K.; Ohnishi, H.; Ali Haghparast, S.M.; Kihara, T.; Miyake, J.

  Biochemical and Biophysical Research Communications, 2020, Peer-reviewed

  
• Transplantation of multiciliated airway cells derived from human iPS cells using an artificial tracheal patch into rat trachea.

  Hideaki Okuyama; Hiroe Ohnishi; Ryosuke Nakamura; Masaru Yamashita; Yo Kishimoto; Ichiro Tateya; Atsushi Suehiro; Shimpei Gotoh; Toshiaki Takezawa; Tatsuo Nakamura; Koichi Omori

  Journal of tissue engineering and regenerative medicine, Jun. 2019, Peer-reviewed

  
• Recurrent laryngeal nerve regeneration using a self-assembling peptide hydrogel

  Masayoshi Yoshimatsu; Ryosuke Nakamura; Yo Kishimoto; Hirofumi Yurie; Yasuyuki Hayashi; Shinji Kaba; Hiroe Ohnishi; Masaru Yamashita; Ichiro Tateya; Koichi Omori

  Laryngoscope, 2019, Peer-reviewed

  
• 基礎研究の将来展望 前庭再生医療研究の将来展望

  田浦 晶子; 大西 弘恵; 西村 幸司; 扇田 秀章; 岡野 高之; 山本 典生; 中川 隆之; 伊藤 壽一

  Equilibrium Research, Oct. 2018

  
• Transplantation of neurons derived from human iPS cells cultured on collagen matrix into guinea-pig cochleae

  Masaaki Ishikawa; Hiroe Ohnishi; Desislava Skerleva; Tatsunori Sakamoto; Norio Yamamoto; Akitsu Hotta; Juichi Ito; Takayuki Nakagawa

  JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, Jun. 2017, Peer-reviewed

  
• 前庭障害に対するiPS細胞移植の可能性

  田浦 晶子; 大西 弘恵

  月刊 臨床神経科学 Clinical neurosciense, Oct. 2016, Invited

  
• Regenerative therapy for vestibular disorders using human induced pluripotent stem cells (iPSCs): neural differentiation of human iPSC-derived neural stem cells after in vitro transplantation into mouse vestibular epithelia

  Akiko Taura; Noriyuki Nakashima; Hiroe Ohnishi; Takayuki Nakagawa; Kazuo Funabiki; Juichi Ito; Koichi Omori

  ACTA OTO-LARYNGOLOGICA, Oct. 2016, Peer-reviewed

  
• Generation of Xeroderma Pigmentosum-A Patient-Derived Induced Pluripotent Stem Cell Line for Use As Future Disease Model

  Hiroe Ohnishi; Takashi Kawasaki; Tomonori Deguchi; Shunsuke Yuba

  CELLULAR REPROGRAMMING, Aug. 2015, Peer-reviewed

  
• Limited hair cell induction from human induced pluripotent stem cells using a simple stepwise method

  Hiroe Ohnishi; Desislava Skerleva; Shin-ichiro Kitajiri; Tatsunori Sakamoto; Norio Yamamoto; Juichi Ito; Takayuki Nakagawa

  NEUROSCIENCE LETTERS, Jul. 2015, Peer-reviewed

  Access this paper :

  
• 段階的分化誘導法を用いたヒトiPS細胞からの内耳有毛細胞様細胞の誘導.

  大西 弘恵; Desislava skerleva; 北尻 真一郎; 坂本 達則; 山本 典生; 伊藤 壽一; 中川 隆之

  ニューロサイエンス研究会会報, 2015

  
• Ex Vivo Expanded Allogeneic Mesenchymal Stem Cells With Bone Marrow Transplantation Improved Osteogenesis in Infants With Severe Hypophosphatasia

  Takeshi Taketani; Chigusa Oyama; Aya Mihara; Yuka Tanabe; Mariko Abe; Tomohiro Hirade; Satoshi Yamamoto; Ryosuke Bo; Rie Kanai; Taku Tadenuma; Yuko Michibata; Soichiro Yamamoto; Miho Hattori; Yoshihiro Katsube; Hiroe Ohnishi; Mari Sasao; Yasuaki Oda; Koji Hattori; Shunsuke Yuba; Hajime Ohgushi; Seiji Yamaguchi

  CELL TRANSPLANTATION, 2015, Peer-reviewed

  
• 1 Effects of mouse utricle stromal tissues on hair cell induction from induced pluripotent stem cells

  Akiko Taura; Hiroe Ohnishi; Shohei Ochi; Fumi Ebisu; Takayuki Nakagawa; Juichi Ito

  BMC NEUROSCIENCE, Nov. 2014, Peer-reviewed

  
• 特集・神経をどう扱うか：再生・再建の工夫：前駆細胞・幹細胞移植.

  大西 弘恵

  JOHNS, Oct. 2014, Invited

  
• Non-neuronal acetylcholine as an endogenous regulator of proliferation and differentiation of Lgr5-positive stem cells in mice

  Toshio Takahashi; Hiroe Ohnishi; Yuki Sugiura; Kurara Honda; Makoto Suematsu; Takashi Kawasaki; Tomonori Deguchi; Takeshi Fujii; Kaoru Orihashi; Yoshitaka Hippo; Takehiro Watanabe; Tohru Yamagaki; Shunsuke Yuba

  FEBS JOURNAL, Oct. 2014, Peer-reviewed

  
• Pluripotent stem cells

  Tatsunori Sakamoto; Koji Nishimura; Hiroe Ohnishi; Takehiro Iki

  Regenerative Medicine for the Inner Ear, 2014, Peer-reviewed

  
• Otic induction

  Hiroe Ohnishi; Tatsunori Sakamoto

  Regenerative Medicine for the Inner Ear, 2014, Peer-reviewed

  
• Reprogramming of Mouse Cochlear Cells by Transcription Factors to Generate Induced Pluripotent Stem Cells

  Xiang-Xin Lou; Takayuki Nakagawa; Koji Nishimura; Hiroe Ohnishi; Norio Yamamoto; Tatsunori Sakamoto; Juichi Ito

  CELLULAR REPROGRAMMING, Dec. 2013, Peer-reviewed

  
• Regulation of cell shape and adhesion by CD34

  Hiroe Ohnishi; Hiroyuki Sasaki; Yoshihiko Nakamura; Shunichi Kato; Kiyoshi Ando; Hisashi Narimatsu; Kouichi Tachibana

  CELL ADHESION & MIGRATION, Sep. 2013, Peer-reviewed

  
• Otospheres derived from neonatal mouse cochleae retain the progenitor cell phenotype after ex vivo expansions

  Xiang-Xin Lou; Takayuki Nakagawa; Hiroe Ohnishi; Koji Nishimura; Juichi Ito

  NEUROSCIENCE LETTERS, Feb. 2013, Peer-reviewed

  
• Allogeneic Mesenchymal Stem Cells for Bone Tissue Engineering: Clinical Experience of Perinatal Hypophosphatasia Patient (Chapter 17).

  Ohnishi H; Katsube Y; Tadokoro M; Yuba S; Ohgushi H

  Stem cells and Bone tissue CRC Press, 2013., Jan. 2013, Peer-reviewed

  
• Identification and developmental expression of leucine-rich repeat-containing G protein-coupled receptor 6 (lgr6) in the medaka fish, Oryzias latipes

  Tomonori Deguchi; Takashi Kawasaki; Hiroe Ohnishi; Shunsuke Yuba; Toshio Takahashi

  DEVELOPMENT GENES AND EVOLUTION, Jul. 2012, Peer-reviewed

  
• A comparative study of induced pluripotent stem cells generated from frozen, stocked bone marrow- and adipose tissue-derived mesenchymal stem cells

  Hiroe Ohnishi; Yasuaki Oda; Tetsuhiro Aoki; Mika Tadokoro; Yoshihiro Katsube; Hajime Ohgushi; Koji Hattori; Shunsuke Yuba

  JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, Apr. 2012, Peer-reviewed

  
• Generation of induced pluripotent stemcells from mesenchymal stromal cells derived fromhuman thirdmolars (method)

  Yasuaki Oda; Hiroe Ohnishi; Shunsuke Yuba; Hajime Ohgushi

  Stem Cells and Cancer Stem Cells, Volume 2: Stem Cells and Cancer Stem Cells, Therapeutic Applications in Disease and Injury: Volume 2, 01 Jan. 2012, Peer-reviewed

  
• Formation of microvilli and phosphorylation of ERM family proteins by CD43, a potent inhibitor for cell adhesion Cell detachment is a potential cue for ERM phosphorylation and organization of cell morphology

  Junko Yamane; Hiroe Ohnishi; Hiroyuki Sasaki; Hisashi Narimatsu; Hajime Ohgushi; Kouichi Tachibana

  CELL ADHESION & MIGRATION, Mar. 2011, Peer-reviewed

  
• Forced Expression of Transcription Factors in Human Mesenchymal cells to Proliferation and Osteogenic differentiation.

  Hiroe Ohnishi; Shunsuke Yuba; Hajime Ohgushi

  Bioceramics Development and Applications, 2011, Invited

  
• Induction of Pluripotent Stem Cells from Human Third Molar Mesenchymal Stromal Cells

  Yasuaki Oda; Yasuhide Yoshimura; Hiroe Ohnishi; Mika Tadokoro; Yoshihiro Katsube; Mari Sasao; Yoko Kubo; Koji Hattori; Shigeru Saito; Katsuhisa Horimoto; Shunsuke Yuba; Hajime Ohgushi

  JOURNAL OF BIOLOGICAL CHEMISTRY, Sep. 2010, Peer-reviewed

  
• Generation of Induced Pluripotent Stem Cells from Human Adipose-Derived Stem Cells Without c-MYC

  Tetsuhiro Aoki; Hiroe Ohnishi; Yasuaki Oda; Mika Tadokoro; Mari Sasao; Hiroyuki Kato; Koji Hattori; Hajime Ohgushi

  TISSUE ENGINEERING PART A, Jul. 2010, Peer-reviewed

  
• Stem cell technology using bioceramics: hard tissue regeneration towards clinical application

  Hiroe Ohnishi; Yasuaki Oda; Hajime Ohgushi

  SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS, Feb. 2010, Invited

  
• The morphological changes in cultured cells caused by Bordetella pertussis adenylate cyclase toxin

  Hiroe Ohnishi; Masami Miyake; Shigeki Kamitani; Yasuhiko Horiguchi

  FEMS MICROBIOLOGY LETTERS, Feb. 2008, Peer-reviewed

  
• Enteropathogenic Escherichia coli, Shigella flexneri, and Listeria monocytogenes recruit a junctional protein, zonula occludens-1, to actin tails and pedestals

  Miyuki Hanajima-Ozawa; Takeshi Matsuzawa; Aya Fukui; Shigeki Kamitani; Hiroe Ohnishi; Akio Abe; Yasuhiko Horiguchi; Masami Miyake

  INFECTION AND IMMUNITY, Feb. 2007, Peer-reviewed

  
• JACOP, a novel plaque protein localizing at the apical junctional complex with sequence similarity to cingulin

  H Ohnishi; T Nakahara; K Furuse; H Sasaki; S Tsukita; M Furuse

  JOURNAL OF BIOLOGICAL CHEMISTRY, Oct. 2004, Peer-reviewed

  
• Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12

  A Kato; H Ohnishi; K Yamamoto; E Furuta; H Tanabe; R Utsumi

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Jun. 2000, Peer-reviewed

  
• Establishment of a radiation-induced vocal fold fibrosis mouse model.

  Yuki Tanigami; Yoshitaka Kawai; Shinji Kaba; Ryuji Uozumi; Hiroe Ohnishi; Tomoko Kita; Koichi Omori; Yo Kishimoto

  Biochemical and biophysical research communications, 23 Apr. 2022, Peer-reviewed

  
• PPARγ Agonist Attenuates Vocal Fold Fibrosis in Rats via Regulation of Macrophage Activation.

  Shinji Kaba; Yoshitaka Kawai; Yuki Tanigami; Hiroe Ohnishi; Tomoko Kita; Masayoshi Yoshimatsu; Koichi Omori; Yo Kishimoto

  The American journal of pathology, 18 Feb. 2022

  Access this paper :

  

Misc.

• 低ホスファターゼ症に対する骨髄移植を併用した間葉系幹細胞移植治療の検討

  竹谷 健; 金井 理恵; 小山 千草; 安部 真理子; 斎藤 敦郎; 鬼形 和道; 福田 誠司; 山口 清次; 三原 綾; 勝部 好裕; 大西 弘恵; 小田 泰昭; 田所 美香; 服部 耕治; 弓場 俊輔; 大串 始

  小児がん, Nov. 2011

  
• 親知らず由来ヒト間葉系細胞からのiPS細胞の樹立

  小田 泰昭; 吉村 康秀; 大西 弘恵; 田所 美香; 勝部 好裕; 笹尾 真理; 服部 耕治; 齊藤 秀; 堀本 勝久; 弓場 俊輔; 大串 始

  日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, Dec. 2010

  
• レトロウイルスベクターを用いたOct3/4・Sox2・Klf4導入によるヒト脂肪組織由来幹細胞からのiPS細胞の樹立のこころみ

  青木 哲宏; 大西 弘恵; 小田 泰昭; 加藤 博之; 大串 始

  移植, Jun. 2010

  
• コラーゲンビトリゲル膜を用いたヒトiPS細胞由来気道上皮細胞のラット鼻腔への移植

  桑田文彦; 大西弘恵; 奥山英晃; 北田有史; 山下勝; 小林正佳; 山本典生; 大森孝一

  日本耳鼻咽喉科学会会報, 2021

  
• 基礎研究の将来展望 前庭再生医療研究の将来展望

  田浦 晶子; 大西 弘恵; 西村 幸司; 扇田 秀章; 中川 隆之; 山本 典生; 岡野 高之; 大森 孝一; 伊藤 壽一

  Equilibrium Research, Jun. 2019

  
• JACOP: A novel cytoplasmic plaque protein localizing at the apical junctional belt

  Hiroe Ohnishi; Takuo Nakahara; Kyoko Furuse; Hiroyuki Sasaki; Shoichiro Tsukita; Mikio Furuse

  CELL STRUCTURE AND FUNCTION, May 2004

  

Presentations

• ヒトiPS細胞由来間葉系幹細胞移植によるX-SCIDラット甲状軟骨再生研究

  大西 弘恵

  第15回 ラットリサーチ研究会, 18 Feb. 2022, Invited

  
• Trial for Improvement of Hair Cell Induction Method from Human Induced Pluripotent Stem Cell.

  Ohnishi H; Skerleva D; Okuyama H; Miyamoto T; Matsuura S; Kirino K; Yamamoto N; Ito J; Omori K; Saito M; Tsukita K; Inoue H; Nakagawa T

  Association for Research in Otolaryngology 42th MidWinter Meeting, 11 Feb. 2019

  
• ビトリゲル上でのヒトiPS細胞からの気道上皮細胞分化誘導

  大西 弘恵; 中村 亮介; 奥山 英晃; 竹澤 俊明; 後藤 慎平; 興梠 陽平; 小西 聡志; 山下 勝; 岸本 曜; 楯谷 一郎; 山本 典生; 中川 隆之; 大森 孝一

  第16回 日本再生医療学会総会, 09 Mar. 2017

  
• 段階的分化誘導法を用いたヒトiPS細胞からの内耳有毛細胞様細胞の誘導

  大西 弘恵; Desislava Skerleva; 北尻 真一郎; 坂本達則; 山本典生; 伊藤壽一; 中川隆之

  第33回 耳鼻咽喉科ニューロサイエンス研究会, 29 Aug. 2015

  
• Differentiation of Human Induced Pluripotent Stem Cells into Glutamatergic Neurons on 3D Scaffolds.

  Ohnishi H; Skerleva D; Sakamoto T; Yamamoto N; Ito J; Nakagawa T

  Inner Ear Biology Workshop, 04 Nov. 2014

  
• Transplantation of terminally differentiated neurons derived from iPS cells into cochleae using the 3D collagen matrix.

  大西 弘恵

  大学院教育コース 64th 再生医療・臓器再建医学コースミーティング、京都大学 iPS細胞研究所, 24 Jan. 2014, Invited

  
• 細胞表在性シアロムチンCD34は微絨毛の形成とintegrin依存性細胞接着を制御する.

  大西弘恵; 佐々木博之; 安藤潔; 成松久; 立花宏一

  第31回日本分子生物学会年会・第81回 日本生化学会大会合同大会, 10 Dec. 2008

  

Books and Other Publications

• 「iPS細胞の安全・高品質な作製技術、第6章、第4節 ヒトiPS細胞からの前庭神経節細胞作製の開発」

  田浦 晶子; 大西 弘恵, Contributor

  株式会社 技術情報協会, Oct. 2016, Not refereed

  
• In Regenerative Medicine for the Inner Ear (Ito J. Ed)

  Sakamoto T; Nishimura K; Ohnishi H; Iki T, Joint work, Pluripotent stem cells. (Chapter 31)

  Springer Japan KK, 2014, Not refereed

  
• Otic induction (Chapter 11). In Regenerative Medicine for the Inner Ear (Ito J. Ed)

  Hiroe Ohnishi; Tatsunori Sakamoto, Contributor

  Springer Japan KK., 2014, Not refereed

  
• Pluripotent stem cells. (Chapter 31). In Regenerative Medicine for the Inner Ear (Ito J. Ed)

  Tatsunori Sakamoto; Kouji Nishimura; Hiroe Ohnishi; Takehiro Iki, Contributor

  Springer Japan KK., 2014, Not refereed

  
• Allogeneic Mesenchymal Stem Cells for Bone Tissue Engineering: Clinical Experience of Perinatal Hypophosphatasia Patient”, Stem cells and Bone tissue, Chapter 17

  Hiroe Ohnishi; Yoshihiro Katsube; Mika Tadokoro; Shunsuke Yuba; Hajime Ohgushi, Contributor

  CRC Press., 2013, Not refereed

  
• Stem cells and Bone tissue

  Ohnishi H; Katsube Y; Tadokoro M; Yuba S; Ohgushi H, Joint work, Allogeneic Mesenchymal Stem Cells for Bone Tissue Engineering: Clinical Experience of Perinatal Hypophosphatasia Patient (Chapter 17)

  CRC Press, 2013, Not refereed

  
• ”Generation of Induced Pluripotent Stem Cells from Mesenchymal Stromal Cells Derived from Human Third Molars.” Stem cells and Cancer Stem Cells Volume 2,

  Yasuaki Oda; Hiroe Ohnishi; Shunsuke Yuba; Hajime Ohgushi, Contributor

  Springer, 2012, Not refereed

  
• ”Lineage-Specific Differentiation of Human Embryonic and Induced Pluripotent Stem Cells: Methods and Protocols Protocol book”, Part II, 12.Mesenchymal stem cells and iPS cells,

  Hiroe Ohnishi; Yasuaki Oda; Hajime Ohgushi, Contributor

  Humana Press, Springer International Publisher Science., 2012, Not refereed

  
• Stem cells and Cancer Stem Cells Volume 12

  Oda Y; Ohnishi H; Yuba S; Ohgushi H, Joint work, Generation of Induced Pluripotent Stem Cells from Mesenchymal Stromal Cells Derived from Human Third Molars (Chapter 9)

  Springer, 2012, Not refereed

  
• Mesenchymal stem cells and iPS cells : Methods and Protocols Protocol book

  Ohnishi H; Oda Y; Ohgushi H, Joint work, (Chapter 12). Lineage-Specific Differentiation of Human Embryonic and Induced Pluripotent Stem Cells, Part II

  Humana Press, Springer International Publisher Science., 2012, Not refereed

  
• 「iPS細胞の産業的応用技術」, 第3章、2.転写因子導入によるヒト間葉系幹細胞の賦活化

  大西弘恵; 大串始, Contributor

  株式会社CMC出版, 2009, Not refereed

  

Industrial Property Rights

• 特許5545460, 特開2010-094077, 特願2008-267678, 細胞のインテグリンを介した接着性の制御方法

  立花宏一; 大西 弘恵

  

Awards

• 22 Feb. 2014

  Association for Research in Otolaryngology, “ARO Graduate Student / Postdoctoral Fellow Travel Award”

External funds: Kakenhi

• Analysis of the mechanism of cochlear planar polarity formation by spatial transcriptome analysis

  Grant-in-Aid for Scientific Research (C)

  Basic Section 56050:Otorhinolaryngology-related

  Kyoto University

  大西 弘恵

  From 01 Apr. 2024, To 31 Mar. 2027, Granted

  ヒトiPS細胞;蝸牛;平面極性;空間トランスクリプトーム;発生

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K12646/
• マクロファージ動態制御による声帯線維化予防技術開発

  Grant-in-Aid for Scientific Research (C)

  Basic Section 56050:Otorhinolaryngology-related

  Kyoto University

  岸本 曜

  From 01 Apr. 2023, To 31 Mar. 2026, Granted

  声帯;瘢痕化;線維化;マクロファージ

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K08935/
• Generation of the transplantation model of human iPS cell-derived airway epithelial sheet into nasal cavity

  Grant-in-Aid for Scientific Research (C)

  Basic Section 56050:Otorhinolaryngology-related

  Kyoto University

  北田 有史

  From 01 Apr. 2023, To 31 Mar. 2026, Granted

  ヒトiPS細胞;気道上皮;鼻腔粘膜;免疫不全ラット;移植

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K08961/
• ヒトiPS細胞由来気道上皮細胞移植における生着効率向上因子の探索

  Grant-in-Aid for Scientific Research (C)

  Basic Section 56050:Otorhinolaryngology-related

  Kyoto University

  桑田 文彦

  From 01 Apr. 2022, To 31 Mar. 2025, Granted

  気管;ヒトiPS細胞;線毛上皮細胞;トランスクリプトーム;免疫不全ラット;気道上皮;移植;トランスクリプトーム解析

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K09666/
• 光刺激人工内耳技術とiPS細胞移植蝸牛神経再生の融合による新規難聴治療法の開発

  Grant-in-Aid for Scientific Research (C)

  Basic Section 56050:Otorhinolaryngology-related

  Kyoto University

  伊藤 壽一

  From 01 Apr. 2022, To 31 Mar. 2026, Granted

  内耳;再生;iPS細胞;人工内耳;光刺激;難聴;光刺激人工内耳;iPS細胞移植

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K09738/
• 光刺激人工内耳が加速させる細胞移植と分化転換からの蝸牛神経再生による新規難聴治療

  Grant-in-Aid for Scientific Research (B)

  Basic Section 56050:Otorhinolaryngology-related

  Kyoto University;Teikyo University

  西村 幸司

  From 01 Apr. 2022, To 31 Mar. 2026, Granted

  蝸牛神経;光遺伝学;遺伝子導入;細胞移植;分化転換;人工内耳

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H03239/
• Elucidation of Inner Ear Development Mechanisms using multi-omics

  Grant-in-Aid for Scientific Research (B)

  Basic Section 56050:Otorhinolaryngology-related

  Kobe City Medical Center General Hospital(First Clinical Division, Second Clinical Division, Third

  山本 典生

  From 01 Apr. 2022, To 31 Mar. 2025, Granted

  内耳発生;網羅的発現解析;遺伝子;タンパク質;エピジェネティクス

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H03234/
• Elucidation of the cochlear development and inner ear pathology with imaging mass-spectrometry for the treatment of sensorineural hearing loss

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields

  Kobe City Medical Center General Hospital(First Clinical Division, Second Clinical Division, Third;Kyoto University

  山本 典生

  From 09 Jul. 2021, To 31 Mar. 2024, Granted

  タンパク質;網羅的発現解析;蝸牛;内耳;発生;感音難聴;イメージング質量分析

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K19564/
• CRISPR/Cas系による新型コロナウイルス感染と気道上皮バリア破壊機構の解明

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields

  Kyoto University

  大森 孝一

  From 09 Jul. 2021, To 31 Mar. 2024, Granted

  COVID-19;CRISPR/Cas9システム;ヒトiPS細胞由来気道上皮移植ラット;SARS-CoV-2 Spikeタンパク質;シュードウイルス;ノックアウトスクリーニング;オミクロン株;CRISPR/CAS9;新型コロナウイルス;気道上皮;バリア破壊

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K19567/
• ヒト多能性幹細胞（iPS細胞）由来内耳オルガノイドの内耳移植による内耳再生研究

  Grant-in-Aid for Scientific Research (C)

  Basic Section 56050:Otorhinolaryngology-related

  Research Institute, Shiga Medical Center

  藤野 清大

  From 01 Apr. 2021, To 31 Mar. 2025, Granted

  内耳;再生;iPS細胞;オルガノイド;ロボット手術;難聴

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K09622/
• 内耳蝸牛三次元構造形成メカニズムの解明と蝸牛型オルガノイド作製への応用

  Grant-in-Aid for Scientific Research (C)

  Basic Section 56050:Otorhinolaryngology-related

  Kyoto University

  大西 弘恵

  From 01 Apr. 2021, To 31 Mar. 2024, Granted

  オルガノイド;Carbonic anhydrase 13;極性;三次元構造;蝸牛;ヒトiPS細胞;SVIP;内耳蝸牛;ゲノム編集;組換えマウス

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K09628/
• Mechanisms for the initial process of hair cell regeneration in avian inner ears

  Grant-in-Aid for Scientific Research (C)

  Basic Section 56050:Otorhinolaryngology-related

  Kyoto University

  中川 隆之

  From 01 Apr. 2020, To 31 Mar. 2024, Granted

  感音難聴;有毛細胞;再生;分化転換;鳥類

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K09708/
• Development of novel therapeutic strategy for upper airway fibrotic lesions by controlling macrophage polarity

  Grant-in-Aid for Scientific Research (C)

  Basic Section 56050:Otorhinolaryngology-related

  Kyoto University

  Yo Kishimoto

  From 01 Apr. 2020, To 31 Mar. 2023, Project Closed

  声帯;喉頭;線維化;マクロファージ;極性;瘢痕;気道線維化病変

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K09730/
• 宇宙環境における内耳前庭の発生維持とバイオメカニクス

  Grant-in-Aid for Scientific Research (C)

  Basic Section 56050:Otorhinolaryngology-related

  Aino University

  田浦 晶子

  From 01 Apr. 2020, To 31 Mar. 2024, Granted

  微小重力;前庭;有毛細胞;宇宙環境;平衡障害

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K09722/
• Elucidation of the develpmental mechanism of the cochlear sensory epithelia based on the single cell analysis

  Grant-in-Aid for Scientific Research (B)

  Basic Section 56050:Otorhinolaryngology-related

  Kyoto University

  Norio Yamamoto

  From 01 Apr. 2019, To 31 Mar. 2022, Project Closed

  網羅的遺伝子発現解析;蝸牛;有毛細胞;単一細胞;内耳発生;遺伝子発現;網羅的遺伝子解析;蝸牛上皮

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H03803/
• Analyses of the mechanisms for development of sensory epithelia in cochleae and vestibula and application to generating the model for deafness using hiPSC

  Grant-in-Aid for Scientific Research (C)

  Basic Section 56050:Otorhinolaryngology-related

  Kyoto University

  Hiroe Ohnishi

  From 01 Apr. 2018, To 31 Mar. 2023, Project Closed

  ヒトiPS細胞;蝸牛;難聴モデル;前庭;オルガノイド;内耳;iPS細胞;内耳オルガノイド;分化誘導;内耳感覚上皮細胞;トランスクリプトーム解析;ゲノム編集

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K09318/
• Development of a new treatment method for hearing loss by fusing cochlear implant technology and cochlear nerve regeneration

  Grant-in-Aid for Challenging Research (Pioneering)

  Research Institute, Shiga Medical Center

  JUICHI ITO

  From 30 Jun. 2017, To 31 Mar. 2022, Project Closed

  人工内耳;蝸牛神経;再生;ヒト多能性幹細胞（iPS）;細胞移植;難聴;内耳;光刺激人工内耳;iPS細胞移植;ロボット手術;オルガノイド;聴神経;有毛細胞;iPS;チャンネルロドプシン;蝸牛神経（聴神経）（;ラセン神経節細胞;ヒト多能性幹細胞（iPS細胞）;iPS細胞由来神経前駆細胞;聴神経（ラセン神経節細胞）;間葉系幹細胞（MSC）;聴力再生;蝸牛有毛細胞;チャネルロドプシン;再生医学;トランスレーショナルリサーチ

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K20307/
• Development of new therapy for balance disorders

  Grant-in-Aid for Scientific Research (C)

  Aino University;Department of Clinical Research, National Hospital Organization Kyoto Medical Center

  Akiko Taura

  From 01 Apr. 2017, To 31 Mar. 2023, Project Closed

  前庭障害;細胞移植;iPS;iPS細胞;移植治療;ｈNSC;移植;hNSC;前庭動眼反射;前庭再生

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K11349/
• Roles of IGF-1-Netrin axis in the cochlea

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Takayuki Nakagawa

  From 01 Apr. 2017, To 31 Mar. 2021, Project Closed

  インスリン様細胞増殖因子１;蝸牛;有毛細胞;シナプス;再生;保護;IGF-1;感音難聴;インスリン様細胞増殖因子;創薬;ネトリン１

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K11325/
• The comprehesive analysis of human hereditary deafness

  Grant-in-Aid for Scientific Research (B)

  Shinshu University

  Shin-ichiro Kitajiri

  From 01 Apr. 2017, To 31 Mar. 2020, Project Closed

  DIAPH1;CLDN14;OTOA;MYO6;POLD1;EPHA2;遺伝性難聴;聴覚;内耳機能;TRIOBP;リポジトリ;新規変異;機能解析;内耳;難聴;遺伝子;MYPT;耳科学;細胞生物学;遺伝学

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17H04345/
• Development of regenerative medicine for head and neck tissues using murine and human iPS cells

  Grant-in-Aid for Scientific Research (B)

  Kyoto University;Fukushima Medical University

  Koichi Omori

  From 01 Apr. 2014, To 31 Mar. 2017, Project Closed

  iPS細胞;気道上皮細胞;筋細胞;分化誘導;移植;軟骨細胞;人工材料

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26293371/
• Elucidation of pathogenic mechanisms of sensorineural hearing loss and establishment of disease model using XP-A patient-derived iPS cells

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Ohnishi Hireo

  From 01 Apr. 2015, To 31 Mar. 2019, Project Closed

  疾患特異的iPS細胞;神経分化誘導;聴神経;内耳感覚上皮組織培養;色素性乾皮症;神経細胞;シナプス形成;共培養;分化誘導

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K10748/
• 血液迷路関門（BLB）障害による平衡障害の病態解明と新規治療法の開発

  Grant-in-Aid for Scientific Research (C)

  Basic Section 56050:Otorhinolaryngology-related

  Aino University

  田浦 晶子

  From 01 Apr. 2024, To 31 Mar. 2029, Granted

  血液迷路関門;前庭;再生医療

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K12686/
• 光刺激人工内耳が加速させる細胞移植と分化転換からの蝸牛神経再生による新規難聴治療

  Grant-in-Aid for Scientific Research (B)

  Basic Section 56050:Otorhinolaryngology-related

  Kyoto University;Teikyo University

  西村 幸司

  From 01 Apr. 2022, To 31 Mar. 2026, Granted

  ラセン神経節;光遺伝学;細胞移植;遺伝子導入;蝸牛神経;分化転換;人工内耳

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K24498/
• Elucidation of inner ear development using multi-omics

  Grant-in-Aid for Scientific Research (B)

  Basic Section 56050:Otorhinolaryngology-related

  Kobe City Medical Center General Hospital(First Clinical Division, Second Clinical Division, Third

  山本 典生

  From 01 Apr. 2022, To 31 Mar. 2025, Granted

  内耳発生;網羅的発現解析;遺伝子;タンパク質;エピジェネティクス

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K24493/

External funds: others

• ヒトiPS細胞を用いた内耳有毛細胞および前駆細胞特異的表面マーカーの探索 ―創薬スクリーニングの為の内耳有毛細胞大量調製を目指して―

  藤原記念財団, 少壮研究者奨励金

  From 2016, To 2016

  大西　弘恵

  中川隆之、山本典生