Basic Information

Professional Memberships

• Society for Neuroscience, USA
• THE PHYSIOLOGICAL SOCIETY OF JAPAN
• THE JAPAN NEUROSCIENCE SOCIETY

Academic Degree

• Master of Science, Neurobiology(Kyoto University)
• Ph.D., Neurobiology(Kyoto University)

High School

• High School

  兵庫県立神戸高等学校（理数コース）

Research History

• From Apr. 2020, To Present

  Kyoto University, Graduate School of Medicine, Department of Drug Discovery Medicine, Program-specific Professor
• From Apr. 2015, To Mar. 2020

  Kyoto University, The Hakubi Center for Advanced Research, Program-specific Associate Professor
• From Apr. 2011, To Mar. 2015

  Kyushu University, Graduate School of Medical Sciences, Specially Appointed Assistant Professor
• From Jul. 2008, To Mar. 2011

  The University of Chicago, Department of Neurobiology, Postdoctoral Scholar
• From Apr. 2007, To Jul. 2008

  Erasmus University Medical Center（Netherlands）, Postdoctoral Fellow
• From Apr. 2006, To Mar. 2008

  JSPS Research Fellowships for Young Scientists

Profile

• Profile

  これまで、京都大学大学院医学研究科 創薬医学講座の特定教授として、脳の炎症と精神疾患発症機序の究明を目指し、げっ歯類とヒトでの精神障害の回復を目指す研究を行ってきました。2025年度からは、高次脳科学講座 神経生物学分野にて、神経変性疾患と精神疾患に関するマウスモデルとヒト病態の機序解明と回復法の開発に取り組んでいます。
  
  研究手法は、電気生理学的手法（多細胞パッチクランプ、樹状突起記録、シナプス伝達、膜興奮性、長期可塑性など、脳切片下の記録）と in vivo 電気生理学記録、イメージング（共焦点顕微鏡、二光子顕微鏡、超解像顕微鏡、小動物核磁気画像法（fMRI））、ミクログリア解析、げっ歯類での行動実験、光・薬理遺伝学的操作、大規模データ解析（細胞レベルイメージングデータ、深層学習、生成AI、ヒトコホートデータ解析（安静時MRI）、超多重抗体染色（空間プロテオミクス）、RNA空間解析、Patch-seqなど）を行っています。他に、細胞レベル数理モデルシミュレーションなども行いました。現在はマルチオミクスと細胞種特異的遺伝子改変を用いた精神疾患と神経変性疾患のプログラミングや人工知能を使った解析および治療法開発を目指しています。特に小脳と脳免疫の機能連関に興味があります。

  
  

  尊敬する多くの先生方の背中を見て、日々チームの皆で研究開発に精励しております。いつか世界中の疾患に苦しまれる多くの方々の力になる研究開発を推進させます。変わらぬご指導ご鞭撻のほど戴けましたら、幸甚にございます。

Language of Instruction

• Japanese
• English

ID,URL

• ORCID ID

  0000-0003-3004-5886

• J-Global ID

  201501068092093432

Website(s) (URL(s))

• http://www.mic.med.kyoto-u.ac.jp/dddm/members/ohtsuki.html
• http://www.mic.med.kyoto-u.ac.jp/dddm_en/members/ohtsuki.html

researchmap URL

• https://researchmap.jp/7000011336

Research

Research Topics, Overview of the research

• Research Topics

  Mental disorders (depression, mood disorders, autism spectrum disorder, schizophrenia)
  Neurodegenerative diseases (ALS, dementia)

Research Areas

• Life sciences, Neuroscience - general

Papers

• Maternal immune activation followed by peripubertal stress combinedly produce reactive microglia and confine cerebellar cognition

  Momoka Hikosaka; Md Sorwer Alam Parvez; Yuki Yamawaki; Souichi Oe; Yuan Liang; Yayoi Wada; Yukie Hirahara; Taro Koike; Hirohiko Imai; Naoya Oishi; Sina M. Schalbetter; Asuka Kumagai; Mari Yoshida; Takeshi Sakurai; Masaaki Kitada; Urs Meyer; Shuh Narumiya; Gen Ohtsuki

  Communications Biology, 03 Mar. 2025, Peer-reviewed, Last author, Corresponding author

  Access this paper :

  
• Sustained antidepressant effects of ketamine metabolite involve GABAergic inhibition-mediated molecular dynamics in aPVT glutamatergic neurons

  Ayako Kawatake-Kuno; Haiyan Li; Hiromichi Inaba; Momoka Hikosaka; Erina Ishimori; Takatoshi Ueki; Yury Garkun; Hirofumi Morishita; Shuh Narumiya; Naoya Oishi; Gen Ohtsuki; Toshiya Murai; Shusaku Uchida

  Neuron, Feb. 2024, Peer-reviewed, Corresponding author

  
• Editorial: Cerebellum-related learning and psychiatric diseases.

  Gen Ohtsuki; Taegon Kim; Peng Sun; Yongjun Chen; Egidio Ugo D'Angelo

  Frontiers in cellular neuroscience, 2023, Peer-reviewed, Invited, Lead author, Corresponding author

  
• 

  Immune-triggered forms of plasticity across brain regions

  Momoka Hikosaka; Takeo Kawano; Yayoi Wada; Tomoki Maeda; Takeshi Sakurai; Gen Ohtsuki

  Frontiers in Cellular Neuroscience, Jun. 2022, Peer-reviewed, Last author, Corresponding author

  
• Insights from a computational analysis of the SARS-CoV-2 Omicron variant: Host-pathogen interaction, pathogenicity, and possible drug therapeutics.

  Md Sorwer Alam Parvez; Manash Kumar Saha; Md Ibrahim; Yusha Araf; Md Taufiqul Islam; Gen Ohtsuki; Mohammad Jakir Hosen

  Immunity, inflammation and disease, Jul. 2022, Corresponding author

  Access this paper :

  
• Acute Cerebellar Inflammation and Related Ataxia: Mechanisms and Pathophysiology.

  Md Sorwer Alam Parvez; Gen Ohtsuki

  Brain sciences, 10 Mar. 2022, Peer-reviewed, Invited, Last author, Corresponding author

  
• 

  Microglia-triggered hypoexcitability plasticity of pyramidal neurons in the rat medial prefrontal cortex

  Yuki Yamawaki; Yayoi Wada; Sae Matsui; Gen Ohtsuki

  Current Research in Neurobiology, Feb. 2022, Peer-reviewed, Invited, Last author, Corresponding author

  Access this paper :

  
• High permeability of the CSF flow in the juvenile mouse brain by brain-wide DCE-MRI

  Yayoi Wada; Hirohiko Imai; Yuki Yamawaki; Md Sorwer Alam Parvez; Sae Matsui; Gen Ohtsuki

  24 Sep. 2021

  
• 

  A Destruction Model of the Vascular and Lymphatic Systems in the Emergence of Psychiatric Symptoms.

  Kohei Segawa; Yukari Blumenthal; Yuki Yamawaki; Gen Ohtsuki

  Biology, 06 Jan. 2021, Peer-reviewed, Invited, Last author, Corresponding author

  
• Psychosis symptoms following aberrant immunity in the brain.

  Akitoshi Ozaki; Yuki Yamawaki; Gen Ohtsuki

  Neural regeneration research, Mar. 2021, Peer-reviewed, Invited, Last author, Corresponding author

  
• 

  Synergistic excitability plasticity in cerebellar functioning

  Gen Ohtsuki; Mari Shishikura; Akitoshi Ozaki

  The FEBS Journal, 11 Nov. 2020, Peer-reviewed, Invited, Lead author, Corresponding author

  
• 

  Modification of synaptic-input clustering by intrinsic excitability plasticity on cerebellar Purkinje cell dendrites

  Gen Ohtsuki

  The Journal of Neuroscience, 21 Nov. 2019, Peer-reviewed, Lead author, Last author, Corresponding author

  
• Microglia-Triggered Plasticity of Intrinsic Excitability Modulates Psychomotor Behaviors in Acute Cerebellar Inflammation

  Yamamoto, M.; Kim, M.; Imai, H.; Itakura, Y.; Ohtsuki, G.

  Cell Reports, 10 Sep. 2019, Peer-reviewed, Last author, Corresponding author

  
• Microglia-triggered hyperexcitability in the cerebellum depresses animal behaviors

  Yamamoto M; Kim M; Imai H; Ohtsuki G

  bioRxiv, Jun. 2018

  
• Synaptic Potential and Plasticity of an SK2 Channel Gate Regulate Spike Burst Activity in Cerebellar Purkinje Cells

  Ohtsuki, G.; Hansel, C.

  iScience, Mar. 2018, Peer-reviewed, Lead author

  Access this paper :

  
• Activity-Dependent Plasticity of Spike Pauses in Cerebellar Purkinje Cells

  Grasselli, G.; He, Q.; Wan, V.; Adelman, J.P.; Ohtsuki, G.; Hansel, C.

  Cell Reports, Mar. 2016, Peer-reviewed

  Access this paper :

  
• The Spontaneous Ataxic Mouse Mutant Tippy is Characterized by a Novel Purkinje Cell Morphogenesis and Degeneration Phenotype

  Shih, E.K.; Sekerková, G.; Ohtsuki, G.; Aldinger, K.A.; Chizhikov, V.V.; Hansel, C.; Mugnaini, E.; Millen, K.J.

  Cerebellum, Jun. 2015, Peer-reviewed

  
• Similarity of visual selectivity among clonally related neurons in visual cortex

  Ohtsuki, G.; Nishiyama, M.; Yoshida, T.; Murakami, T.; Histed, M.; Lois, C.; Ohki, K.

  Neuron, Jul. 2012, Peer-reviewed

  
• SK2 channel modulation contributes to compartment-specific dendritic plasticity in cerebellar Purkinje cells

  Ohtsuki, G.; Piochon, C.; Adelman, J.P.; Hansel, C.

  Neuron, Jul. 2012, Peer-reviewed

  
• Intrinsic plasticity complements long-term potentiation in parallel fiber input gain control in cerebellar Purkinje cells

  Belmeguenai, A.; Hosy, E.; Bengtsson, F.; Pedroarena, C.M.; Piochon, C.; Teuling, E.; He, Q.; Ohtsuki, G.; De Jeu, M.T.G.; Elgersma, Y.; De Zeeuw, C.I.; Jörntell, H.; Hansel, C.

  Journal of Neuroscience, Oct. 2010, Peer-reviewed

  
• Purkinje cell-specific knockout of the protein phosphatase PP2B impairs potentiation and cerebellar motor learning

  Schonewille, M.; Belmeguenai, A.; Koekkoek, S.K.; Houtman, S.H.; Boele, H.J.; van Beugen, B.J.; Gao, Z.; Badura, A.; Ohtsuki, G.; Amerika, W.E.; Hosy, E.; Hoebeek, F.E.; Elgersma, Y.; Hansel, C.; De Zeeuw, C.I.

  Neuron, Aug. 2010, Peer-reviewed

  
• Purkinje cell NMDA receptors assume a key role in synaptic gain control in the mature cerebellum

  Piochon, C.; Levenes, C.; Ohtsuki, G.; Hansel, C.

  Journal of Neuroscience, 2010, Peer-reviewed

  
• Climbing fiber signaling and cerebellar gain control

  Gen Ohtsuki; Claire Piochon; Christian Hansel

  FRONTIERS IN CELLULAR NEUROSCIENCE, Jul. 2009, Peer-reviewed

  
• Bidirectional plasticity at developing climbing fiber-Purkinje neuron synapses

  Gen Ohtsuki; Tomoo Hirano

  EUROPEAN JOURNAL OF NEUROSCIENCE, Dec. 2008, Peer-reviewed

  
• Enhancement of Both Long-Term Depression Induction and Optokinetic Response Adaptation in Mice Lacking Delphilin

  Tomonori Takeuchi; Gen Ohtsuki; Takashi Yoshida; Masahiro Fukaya; Tasuku Wainai; Manami Yamashita; Yoshito Yamazaki; Hisashi Mori; Kenji Sakimura; Susumu Kawamoto; Masahiko Watanabe; Tomoo Hirano; Masayoshi Mishina

  PLOS ONE, May 2008, Peer-reviewed

  
• Threshold regulation of cerebellar LTD is critical for motor learning efficiency

  Tomonori Takeuchi; Gen Ohtsuki; Takashi Yoshida; Masahiro Fukaya; Tasuku Wainai; Hisashi Mori; Kenji Sakimura; Susumu Kawamoto; Masahiko Watanabe; Tomoo Hirano; Masayoshi Mishina

  NEUROSCIENCE RESEARCH, 2006, Peer-reviewed

  
• Enhanced inhibitory synaptic transmission in the cerebellar molecular layer of the GluR delta 2 knock-out mouse

  G Ohtsuki; S Kawaguchi; M Mishina; T Hirano

  JOURNAL OF NEUROSCIENCE, Dec. 2004, Peer-reviewed

  
• Oscillating Purkinje neuron activity causing involuntary eye movement in a mutant mouse deficient in the glutamate receptor delta 2 subunit

  T Yoshida; A Katoh; G Ohtsuki; M Mishina; T Hirano

  JOURNAL OF NEUROSCIENCE, Mar. 2004, Peer-reviewed

  

Misc.

• Threshold regulation of LTD at PF-PC synapses is crucial for the efficiency of OKR gain-increase adaptation

  Tomonori Takeuchi; Gen Ohtsuki; Takashi Yoshida; Masahiro Fukaya; Masahiko Watanabe; Tomoo Hitano; Masayoshi Mishina

  JOURNAL OF PHARMACOLOGICAL SCIENCES, 2007

  
• Facilitated cerebellar LTD and OKR adaptation in mice lacking Delphilin

  T Takeuchi; T Yoshida; G Ohtsuki; T Hirano; T Wainai; M Fukaya; M Watanabe; H Mori; K Sakimura; S Kawamoto; M Mishina

  JOURNAL OF PHARMACOLOGICAL SCIENCES, 2006

  

Presentations

• Immune-triggered excitability plasticity and psychiatric disease models

  Ohtsuki G

  The 25th Annual Meeting of the Korean Society for Brain and Neural Sciences (Incheon, Korea), Invited

  
• Plasticity of the intrinsic excitability in the acute inflammation and psychiatric disease

  Ohtsuki G

  The 99th Annual Meeting of the Physiological Society of Japan (Sendai, Japan)

  
• Intrinsic plasticity of the Purkinje-cell dendrites and immune stress

  Ohtsuki G

  1st China-Japan-Korea International Meeting and 44th Annual Meeting of the Japan Neuroscience Society (Kobe, Japan), Invited

  
• Excitability plasticity and mental depressiveness during acute inflammation in the cerebellum

  大槻 元

  生理学研究所研究会 「ミクロからマクロに至る脳の構造と機能のダイナミクス」, 07 Nov. 2019

  
• Aberrant Immunity in the Cerebellum Modulates Our Mood

  Gen Ohtsuki

  Neuroscience2019 in SfN - Press Conferrence, 21 Oct. 2019, Society for Neuroscience, Invited

  
• Excitability plasticity and mental depressiveness during acute inflammation in the cerebellum

  Gen Ohtsuki

  WMU-IFAR, Wenzhou Medical University Institute for Advanced Research, 15 Sep. 2019, Jian Yang and Zi-Bing JIN, Invited

  
• Functional Diversification in Clonal Cells after Eye-Opening in Mouse Visual Cortex

  Gen Ohtsuki

  京都大学大学院 理学研究科 ミニシンポジウム, Jul. 2015, 田川義晃

  
• Functional organization of clonally related neurons in mouse visual cortex during development

  Ohtsuki G; Nishiyama M; Yoshida T; Murakami T; Histed M; Lois C; Ohki K

  日本神経科学学会, Jul. 2013, 日本神経科学学会

  
• Activity-dependent control of signaling gains in neuronal dendrites

  Gen Ohtsuki

  Gordon Research Seminar Dendrites: Molecules, Structure & Function, 12 Mar. 2011, Georgeann S. Sack and Denise Cook

  
• Long-term plasticity at cerebellar climbing fiber-Purkinje cell synapses

  Gen Ohtsuki

  Long-term plasticity at cerebellar climbing fiber-Purkinje cell synapses, 2010, ノースウエスタン大学 医学部, Invited

  

Books and Other Publications

• 生体の科学 Vol.72 No.1 [特集]小脳研究の未来 プルキンエ細胞興奮性の可塑性

  大槻 元; 山脇優輝; 谷垣宏亮, Joint work

  医学書院, Feb. 2021

  
• Modulation of the neural function based on the plasticity of intrinsic excitability in the cerebellum

  Gen Ohtsuki, Contributor, Neurophysiology

  (公財)ブレインサイエンス振興財団, Jun. 2019, Not refereed

  

External funds: Kakenhi

• Dimensional approaches to psychiatric disorders associated with circuit abnormalities induced by genetic and environmental insults combined with inflammation in the brain

  Grant-in-Aid for Scientific Research (B)

  Basic Section 51030:Pathophysiologic neuroscience-related

  Kyoto University

  Takeshi Sakurai

  From 01 Apr. 2019, To 31 Mar. 2022, Project Closed

  社会性ストレス;思春期;マウスモデル;脳内炎症;精神疾患

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H03547/
• Analysis of the functional development of clonally-related neurons in the visual cortex

  Grant-in-Aid for Young Scientists (A)

  Kyoto University;Kyushu University

  Gen Ohtsuki

  From 01 Apr. 2014, To 31 Mar. 2018, Project Closed

  クローン細胞;遺伝子改変マウス;大脳皮質;一次視覚野;生体下Ca2+イメージング;細胞位置機能解析;機能発達;３次元位置機能解析;大脳皮質一次視覚野;多細胞同時パッチクランプ;3次元位置機能解析;4細胞同時パッチクランプ記録

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26710002/
• 複合炎症精神疾患モデルマウスの小脳機能低下の神経生理学的機序解明

  Grant-in-Aid for Scientific Research (C)

  Basic Section 46010:Neuroscience-general-related

  Kyoto University

  大槻 元

  From 01 Apr. 2023, To 31 Mar. 2026, Granted

  複合炎症ストレス;精神疾患モデル;小脳;シナプス可塑性;内因性興奮性可塑性;学習障害;妊娠期母体免疫活性化;発達期社会敗北ストレス;精神疾患;免疫誘導型可塑性

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K05978/
• 心の健康増進をめざしたストレス感受性の生物学的基盤の解明

  Grant-in-Aid for Scientific Research (B)

  Basic Section 59040:Nutrition science and health science-related

  Nagoya City University;Kyoto University

  内田 周作

  From 01 Apr. 2022, To 31 Mar. 2025, Granted

  ストレス;神経回路;うつ病;ストレス脆弱性;レジリエンス;個体差;エピジェネティクス;アンヘドニア;社交性;分子メカニズム

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K24789/

External funds: others

• 認知症克服に向けた脳のレジリエンスを支えるリザバー機能とその増強法の開発研究（代表: 伊佐正）

  国立研究開発法人日本医療研究開発機構, ムーンショット型研究開発事業

  From 01 Nov. 2024, To 31 Sep. 2027

  分担
• 妊娠・発達期複合炎症ストレスに起因する精神疾患症状・学習障害の疾患関連免疫細胞を標的とした治療法の研究開発

  PRIME

  国立研究開発法人日本医療研究開発機構, 令和6年度 PRIME「ストレスへの応答と疾病発症に至るメカニズムの解明」

  From 01 Oct. 2024, To 31 Mar. 2028

  代表
• イメージングマスサイトメトリーを用いた孤発性ALSにおけるシングルセルプロテオミクス解析

  武田薬品工業, 【武田薬品工業共催 学内限定公募】ニューロサイエンス領域 学内限定研究助成

  From 01 Apr. 2023, To 31 Mar. 2025

  代表
• 脈管・免疫系の発達と破綻に着目した精神疾患発症機序

  生命科学研究助成

  公益財団法人 武田科学振興財団, 武田科学振興財団　2021年度生命科学研究助成

  From 01 Apr. 2021, To 31 Mar. 2023

  代表
• 発達期小脳炎症による精神病様症状の免疫操作による回復

  自然科学研究助成 一般助成

  公益財団　三菱財団, 三菱財団　第51回（2020年度）自然科学研究助成 一般助成

  From 01 Apr. 2020, To 31 Mar. 2023

  代表
• 脳内免疫-神経連関に着目した脳炎発症機序の解明とその抑制

  第１回次世代育成支援研究助成金

  内藤記念科学振興財団, 内藤記念科学振興財団　第１回次世代育成支援研究助成金

  From 01 Apr. 2017, To 31 Mar. 2020
• 脳炎に関わる免疫-神経連関を介した興奮性可塑性機序の全容解明

  研究奨励金Ⅰ

  東京生化学研究会, 東京生化学研究会、平成28年度 研究助成金・研究奨励金Ⅰ

  From 01 Apr. 2017, To 31 Mar. 2018

  代表
• 神経細胞樹状突起の共起性シナプス応答解析

  ブレインサイエンス振興財団, ブレインサイエンス振興財団、第30回 研究助成

  From 01 Apr. 2016, To 31 Mar. 2017

  代表
• クローン細胞に着目した学習思考原理の解明と脳機能不全への応用

  白眉プロジェクト運営費

  京都大学, 京都大学　白眉プロジェクト

  From 01 Apr. 2015, To 31 Mar. 2020

  代表
• 神経幹細胞への光遺伝学導入による脳梗塞後の機能回復

  興和生命科学振興財団, 興和生命科学振興財団、平成26年度 研究助成

  From 01 Apr. 2014, To 31 Mar. 2015

  代表

Education

Teaching subject(s)

• From 01 Apr. 2025, To 31 Mar. 2026

  RNA Informatics Exercise

  E072, Fall, Graduate School of Medicine, 2
• From Apr. 2015, To Mar. 2016

  Interdisciplinary Life Science Course

  Fall, 理学研究科
• From Apr. 2016, To Mar. 2017

  Interdisciplinary Life Science Course

  Fall, 理学研究科
• From Apr. 2017, To Mar. 2018

  Interdisciplinary Life Science Course

  Fall, 理学研究科

Academic, Social Contribution

Academic Contribution

• Cerebellum-Related Learning and Psychiatric Diseases: Topic Editor (Frontiers In Cellular Neuroscience)

  Other

  Gen Ohtsuki, Taegon Kim, Peng Sun, Yong-Jun Chen & Egidio D‘Angelo