Basic Information

Affiliated programs (koza)

• Graduate School of Medicine, 医学系専攻 遺伝医学講座　神経・細胞薬理学分野, 教授

Faculty

• 医学部

Professional Memberships

• 日本生物物理学会
• 日本分子生物学会
• 日本薬理学会
• 日本生化学会
• 日本細胞生物学会

Academic Degree

• 博士（医学）(京都大学)

Academic Resume (Graduate Schools)

• 京都大学, 大学院医学研究科博士課程生理系専攻, 研究指導認定退学
• 京都大学, 大学院医学研究科博士課程生理系専攻, 修了

Academic Resume (Undergraduate School/Majors)

• 京都大学, 医学部医学科, 卒業
• 京都大学, 医学部医学進学課程, 修了

Research History

• From Jan. 2015, To Present

  Kyoto University Graduate School of Biostudies, Laboratory of Single-Molecule Cell Biology, Professor
• From Jul. 2014, To Present

  Kyoto University Faculty of Medicine, Pharmacology, Professor
• From Apr. 2010, To Jun. 2014

  Tohoku University Graduate School of Life Sciences, Department of Biomolecular Sciences, Division of Molecular Biosystems,Laboratory of Single-Molecule Cell Biology, Professor
• From Jun. 2002, To Mar. 2010

  Kyoto University Faculty of Medicine, Pharmacology, Associate Professor
• From 1999, To 2002

  Harvard University
• From 1999, To 2002

  Harvard Medical School
• From 01 Jun. 1991

  -, -
• From 01 Jun. 1990

  -, -

Language of Instruction

• English

ID,URL

• J-Global ID

  201001069870112040

• Researcher number

  80303816

• External link

  http://www.med.kyoto-u.ac.jp/en/organization-staff/research/doctoral_course/r-020/

Website(s) (URL(s))

• http://www.pharm2.med.kyoto-u.ac.jp/

researchmap URL

• https://researchmap.jp/read0152050

Research

Research Topics, Overview of the research

• Research Topics

  Elucidation of cell locomotion and mechanotransduction mechanisms and pursuit of druggable targets by real-time and super-resolution monitoring of intracellular molecular behavior

• Overview of the research

  By applying visualization techniques such as live-cell single-molecular imaging and original multi-target high-density super resolution microscopy IRIS, I aim at elucidating cell locomotion and mechanotransduction mechanisms regulated by actin remodeling processes and its major polymerizing factor, formin homology proteins. I also elucidate the regulatory mechanisms of cancer, immune and nervous systems by directly capturing the actions of signaling molecules in the complex living systems, and aim to reveal unforeseen mechanisms of action of drugs and pursue druggable targets.

Research Interests

• Brownian ratchet force sensor
• kinase inhibitor
• Single-molecule imaging
• Pharmacology
• Cell Biology
• Super-resolution microscopy
• 分子標的薬
• メカノセンス
• フォルミンファミリー
• 細胞分子イメージング
• target-based drug
• mechanotransduction
• formin homology proteins
• live-cell single-molecule imaging

Research Areas

• Life sciences, Cell biology

Papers

• Force transmission by retrograde actin flow-induced dynamic molecular stretching of Talin

  Sawako Yamashiro; David M. Rutkowski; Kelli Ann Lynch; Ying Liu; Dimitrios Vavylonis; Naoki Watanabe

  Nature Communications, 20 Dec. 2023, Peer-reviewed, Last author

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• Protocol to generate fast-dissociating recombinant antibody fragments for multiplexed super-resolution microscopy

  Qianli Zhang; Akitoshi Miyamoto; Naoki Watanabe

  STAR Protocols, Sep. 2023

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• Possible application of IRIS multiplexed super-resolution microscopy for pathological analysis

  Akitoshi Miyamoto; Naoki Watanabe

  Precision Medicine, Jul. 2023, Invited, Last author

  
• Brownian Ratchet-based Force Sensor, Lamellipodium

  Naoki Watanabe

  Seibutsu Butsuri, May 2023, Peer-reviewed, Invited, Lead author, Last author, Corresponding author

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• Brownian ratchet force sensor at the contacting point between F-actin barbed end and lamellipodium tip plasma membrane

  Naoki Watanabe

  Plasma Membrane Shaping, 2023, Peer-reviewed, Invited, Lead author, Last author, Corresponding author

  
• Engineered fast-dissociating antibody fragments for multiplexed super-resolution microscopy

  Qianli Zhang; Akitoshi Miyamoto; Shin Watanabe; Takao Arimori; Masanori Sakai; Madoka Tomisaki; Tai Kiuchi; Junichi Takagi; Naoki Watanabe

  Cell Reports Methods, Sep. 2022, Peer-reviewed, Last author, Corresponding author

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• Fast-dissociating but highly specific antibodies are novel tools in biology, especially useful for multiplex super-resolution microscopy.

  Takushi Miyoshi; Thomas B Friedman; Naoki Watanabe

  STAR protocols, 17 Dec. 2021, Last author

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• Probing Actin Polymerization-Driven Cell Motility Machinery by Single-Molecule Imaging

  WATANABE Naoki; HIGASHIDA Chiharu; MIYOSHI Takushi

  Biophysics, 25 Nov. 2005

  
• A mechanism with severing near barbed ends andannealing explains structure and dynamics of dendriticactin networks.

  Danielle Holz; Aaron R Hall; Eiji Usukura; Sawako Yamashiro; Naoki Watanabe; Dimitrios Vavylonis

  eLife, 07 Jun. 2022, Peer-reviewed

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• Paradoxical activation of c-Src as a drug-resistant mechanism.

  Makio Higuchi; Kenichi Ishiyama; Masahiro Maruoka; Ryosuke Kanamori; Akifumi Takaori-Kondo; Naoki Watanabe

  Cell reports, 23 Mar. 2021, Peer-reviewed, Last author, Corresponding author

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• Semi-automated single-molecule microscopy screening of fast-dissociating specific antibodies directly from hybridoma cultures.

  Takushi Miyoshi; Qianli Zhang; Takafumi Miyake; Shin Watanabe; Hiroe Ohnishi; Jiji Chen; Harshad D Vishwasrao; Oisorjo Chakraborty; Inna A Belyantseva; Benjamin J Perrin; Hari Shroff; Thomas B Friedman; Koichi Omori; Naoki Watanabe

  Cell reports, 02 Feb. 2021, Peer-reviewed, Last author, Corresponding author

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• mDia1/3-dependent actin polymerization spatiotemporally controls LAT phosphorylation by Zap70 at the immune synapse.

  D Thumkeo; Y Katsura; Y Nishimura; P Kanchanawong; K Tohyama; T Ishizaki; S Kitajima; C Takahashi; T Hirata; N Watanabe; M F Krummel; S Narumiya

  Science advances, Jan. 2020, Peer-reviewed

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• Lamellipodium tip actin barbed ends serve as a force sensor.

  Koseki K; Taniguchi D; Yamashiro S; Mizuno H; Vavylonis D; Watanabe N

  Genes to cells : devoted to molecular & cellular mechanisms, Nov. 2019, Peer-reviewed, Last author, Corresponding author

  
• RECK in Neural Precursor Cells Plays a Critical Role in Mouse Forebrain Angiogenesis.

  Li H; Miki T; Almeida GM; Hanashima C; Matsuzaki T; Kuo CJ; Watanabe N; Noda M

  iScience, Sep. 2019, Peer-reviewed

  
• Quantitative high-precision imaging of myosin-dependent filamentous actin dynamics.

  Yamashiro S; Watanabe N

  Journal of muscle research and cell motility, Jul. 2019, Peer-reviewed, Last author

  
• Convection-Induced Biased Distribution of Actin Probes in Live Cells.

  Yamashiro S; Taniguchi D; Tanaka S; Kiuchi T; Vavylonis D; Watanabe N

  Biophysical journal, Jan. 2019, Peer-reviewed, Last author, Corresponding author

  
• Mechanostress resistance involving formin homology proteins: G- and F-actin homeostasis-driven filament nucleation and helical polymerization-mediated actin polymer stabilization.

  Watanabe N; Tohyama K; Yamashiro S

  Biochemical and biophysical research communications, Oct. 2018, Peer-reviewed, Lead author, Corresponding author

  
• mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility.

  Satoko Sakamoto; Dean Thumkeo; Hiroshi Ohta; Zhen Zhang; Shuangru Huang; Pakorn Kanchanawong; Takayoshi Fuu; Sadanori Watanabe; Kentaro Shimada; Yoshitaka Fujihara; Shosei Yoshida; Masahito Ikawa; Naoki Watanabe; Mitinori Saitou; Shuh Narumiya

  PLoS biology, Sep. 2018, Peer-reviewed

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• MTSS1 Regulation of Actin-Nucleating Formin DAAM1 in Dendritic Filopodia Determines Final Dendritic Configuration of Purkinje Cells.

  Kawabata Galbraith K; Fujishima K; Mizuno H; Lee SJ; Uemura T; Sakimura K; Mishina M; Watanabe N; Kengaku M

  Cell reports, Jul. 2018, Peer-reviewed

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• Helical rotation of the diaphanous-related formin mDia1 generates actin filaments resistant to cofilin

  Hiroaki Mizuno; Kotaro Tanaka; Sawako Yamashiro; Akihiro Narita; Naoki Watanabe

  Proceedings of the National Academy of Sciences of the United States of America, 29 May 2018, Peer-reviewed, Last author, Corresponding author

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• Myosin-dependent actin stabilization as revealed by single-molecule imaging of actin turnover.

  Yamashiro S; Tanaka S; McMillen LM; Taniguchi D; Vavylonis D; Watanabe N

  Molecular biology of the cell, May 2018, Peer-reviewed, Last author

  
• Cell protrusion and retraction driven by fluctuations in actin polymerization: A two-dimensional model

  Gillian L. Ryan; Danielle Holz; Sawako Yamashiro; Daisuke Taniguchi; Naoki Watanabe; Dimitrios Vavylonis

  CYTOSKELETON, Dec. 2017, Peer-reviewed

  
• Overview of Single-Molecule Speckle (SiMS) Microscopy and Its Electroporation-Based Version with Efficient Labeling and Improved Spatiotemporal Resolution

  Sawako Yamashiro; Naoki Watanabe

  SENSORS, Jul. 2017, Peer-reviewed, Invited, Last author

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• An Infrared Actin Probe for Deep-Cell Electroporation-Based Single-Molecule Speckle (eSiMS) Microscopy

  Sawako Yamashiro; Naoki Watanabe

  SENSORS, Jul. 2017, Peer-reviewed, Last author

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• Constitutive activation of DIA1 (DIAPH1) via C-terminal truncation causes human sensorineural hearing loss

  Takehiko Ueyama; Yuzuru Ninoyu; Shin-ya Nishio; Takushi Miyoshi; Hiroko Torii; Koji Nishimura; Kazuma Sugahara; Hideaki Sakata; Dean Thumkeo; Hirofumi Sakaguchi; Naoki Watanabe; Shin-ichi Usami; Naoaki Saito; Shin-ichiro Kitajiri

  EMBO MOLECULAR MEDICINE, Nov. 2016, Peer-reviewed

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• Multitarget super-resolution microscopy with high-density labeling by exchangeable probes

  Tai Kiuchi; Makio Higuchi; Akihiro Takamura; Masahiro Maruoka; Naoki Watanabe

  NATURE METHODS, Aug. 2015, Peer-reviewed, Last author, Corresponding author

  
• An easy-to-use single-molecule speckle microscopy enabling nanometer-scale flow and wide-range lifetime measurement of cellular actin filaments

  Sawako Yamashiro; Hiroaki Mizuno; Naoki Watanabe

  BIOPHYSICAL METHODS IN CELL BIOLOGY, 2015, Peer-reviewed, Invited, Last author

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• A new link between the retrograde actin flow and focal adhesions

  Sawako Yamashiro; Naoki Watanabe

  JOURNAL OF BIOCHEMISTRY, Nov. 2014, Peer-reviewed, Invited, Last author

  
• New single-molecule speckle microscopy reveals modification of the retrograde actin flow by focal adhesions at nanometer scales

  Sawako Yamashiro; Hiroaki Mizuno; Matthew B. Smith; Gillian L. Ryan; Tai Kiuchi; Dimitrios Vavylonis; Naoki Watanabe

  MOLECULAR BIOLOGY OF THE CELL, Apr. 2014, Peer-reviewed, Last author, Corresponding author

  
• Rotational Movement of Formins Evaluated by Using Single-Molecule Fluorescence Polarization

  Hiroaki Mizuno; Naoki Watanabe

  RECONSTITUTING THE CYTOSKELETON, 2014, Peer-reviewed, Invited

  
• Molecular viewing of actin polymerizing actions and beyond: Combination analysis of single-molecule speckle microscopy with modeling, FRAP and s-FDAP (sequential fluorescence decay after photoactivation)

  Naoki Watanabe; Sawako Yamashiro; Dimitrios Vavylonis; Tai Kiuchi

  Development Growth and Differentiation, May 2013, Peer-reviewed, Invited, Lead author, Corresponding author

  
• Can filament treadmilling alone account for the F-actin turnover in lamellipodia?

  Takushi Miyoshi; Naoki Watanabe

  CYTOSKELETON, Apr. 2013, Peer-reviewed

  
• F-and G-actin homeostasis regulates mechanosensitive actin nucleation by formins

  Chiharu Higashida; Tai Kiuchi; Yushi Akiba; Hiroaki Mizuno; Masahiro Maruoka; Shuh Narumiya; Kensaku Mizuno; Naoki Watanabe

  Nature Cell Biology, Apr. 2013, Peer-reviewed, Last author, Corresponding author

  
• Distributed actin turnover in the lamellipodium and FRAP kinetics

  Matthew B. Smith; Tai Kiuchi; Naoki Watanabe; Dimitrios Vavylonis

  Biophysical Journal, 08 Jan. 2013, Peer-reviewed

  
• Image Analysis Tools to Quantify Cell Shape and Protein Dynamics near the Leading Edge

  Gillian L. Ryan; Naoki Watanabe; Dimitrios Vavylonis

  CELL STRUCTURE AND FUNCTION, 2013, Peer-reviewed

  
• RhoD activated by fibroblast growth factor induces cytoneme-like cellular protrusions through mDia3C

  Kazuhisa Koizumi; Kazunori Takano; Akiko Kaneyasu; Haruko Watanabe-Takano; Emi Tokuda; Tomoyuki Abe; Naoki Watanabe; Tadaomi Takenawa; Takeshi Endo

  MOLECULAR BIOLOGY OF THE CELL, Dec. 2012, Peer-reviewed

  
• Excitable Actin Dynamics in Lamellipodial Protrusion and Retraction

  Gillian L. Ryan; Heather M. Petroccia; Naoki Watanabe; Dimitrios Vavylonis

  BIOPHYSICAL JOURNAL, Apr. 2012, Peer-reviewed

  
• A review of models of fluctuating protrusion and retraction patterns at the leading edge of motile cells

  Gillian L. Ryan; Naoki Watanabe; Dimitrios Vavylonis

  CYTOSKELETON, Apr. 2012, Peer-reviewed

  
• Diffusion, capture and recycling of SCAR/WAVE and Arp2/3 complexes observed in cells by single-molecule imaging

  Arthur Millius; Naoki Watanabe; Orion D. Weiner

  JOURNAL OF CELL SCIENCE, Mar. 2012, Peer-reviewed

  
• Abi-1-bridged tyrosine phosphorylation of VASP by Abelson kinase impairs association of VASP to focal adhesions and regulates leukaemic cell adhesion

  Masahiro Maruoka; Mizuho Sato; Yunfeng Yuan; Masayoshi Ichiba; Ryosuke Fuji; Takuya Ogawa; Norihiro Ishida-Kitagawa; Tatsuo Takeya; Naoki Watanabe

  BIOCHEMICAL JOURNAL, Feb. 2012, Peer-reviewed

  
• mDia1 and formins: Screw cap of the actin filament

  Hiroaki Mizuno; Naoki Watanabe

  Biophysics (Japan), 2012, Peer-reviewed, Last author, Corresponding author

  
• Liprin-alpha controls stress fiber formation by binding to mDia and regulating its membrane localization

  Satoko Sakamoto; Toshimasa Ishizaki; Katsuya Okawa; Sadanori Watanabe; Takatoshi Arakawa; Naoki Watanabe; Shuh Narumiya

  JOURNAL OF CELL SCIENCE, Jan. 2012, Peer-reviewed

  
• FLUORESCENCE SINGLE-MOLECULE IMAGING OF ACTIN TURNOVER AND REGULATORY MECHANISMS

  Naoki Watanabe

  METHODS IN ENZYMOLOGY, VOL 505: IMAGING AND SPECTROSCOPIC ANALYSIS OF LIVING CELLS, 2012, Invited, Lead author, Last author, Corresponding author

  
• Actin-Independent Behavior and Membrane Deformation Exhibited by the Four-Transmembrane Protein M6a

  Yasufumi Sato; Naoki Watanabe; Nanae Fukushima; Sakura Mita; Tatsumi Hirata

  PLOS ONE, Dec. 2011, Peer-reviewed

  
• Interactive, Computer-Assisted Tracking of Speckle Trajectories in Fluorescence Microscopy: Application to Actin Polymerization and Membrane Fusion

  Matthew B. Smith; Erdem Karatekin; Andrea Gohlke; Hiroaki Mizuno; Naoki Watanabe; Dimitrios Vavylonis

  BIOPHYSICAL JOURNAL, Oct. 2011, Peer-reviewed

  
• Live-cell imaging of G-actin dynamics using sequential FDAP.

  Kiuchi T; Nagai T; Ohashi K; Watanabe N; Mizuno K

  Bioarchitecture, Sep. 2011, Peer-reviewed

  
• Rotational Movement of the Formin mDia1 Along the Double Helical Strand of an Actin Filament

  Hiroaki Mizuno; Chiharu Higashida; Yunfeng Yuan; Toshimasa Ishizaki; Shuh Narumiya; Naoki Watanabe

  SCIENCE, Jan. 2011, Peer-reviewed, Last author, Corresponding author

  
• Inside view of cell locomotion through single-molecule: fast F-/G-actin cycle and G-actin regulation of polymer restoration

  Naoki Watanabe

  PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, Jan. 2010, Peer-reviewed, Invited, Lead author, Last author, Corresponding author

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• Migration without a clutch

  Shuh Narumiya; Naoki Watanabe

  NATURE CELL BIOLOGY, Dec. 2009, Invited, Last author

  
• Cdc42 and Rac Family GTPases Regulate Mode and Speed but Not Direction of Primary Fibroblast Migration during Platelet-Derived Growth Factor-Dependent Chemotaxis

  James Monypenny; Daniel Zicha; Chiharu Higashida; Fabian Oceguera-Yanez; Shuh Narumiya; Naoki Watanabe

  MOLECULAR AND CELLULAR BIOLOGY, May 2009, Peer-reviewed, Last author

  
• An Order of Magnitude Faster AIP1-Associated Actin Disruption than Nucleation by the Arp2/3 Complex in Lamellipodia

  Takahiro Tsuji; Takushi Miyoshi; Chiharu Higashida; Shuh Narumiya; Naoki Watanabe

  PLOS ONE, Mar. 2009, Peer-reviewed, Last author, Corresponding author

  
• A Possible Role of Homeostasis Between Monomeric and Filamentous Actin in Filament Nucleation Revealed by Pharmacokinetic Modeling

  Naoki Watanabe; Chiharu Higashida

  SYSTEMS BIOLOGY: THE CHALLENGE OF COMPLEXITY, 2009, Peer-reviewed, Lead author, Corresponding author

  
• Imatinib Mesylate (STI571)-Induced Cell Edge Translocation of Kinase-Active and Kinase-Defective Abelson Kinase: Requirements of Myristoylation and src Homology 3 Domain

  Akiko Fujita; Tomoyuki Shishido; Yunfeng Yuan; Eiji Inamoto; Shuh Narumiya; Naoki Watanabe

  MOLECULAR PHARMACOLOGY, Jan. 2009, Peer-reviewed, Last author, Corresponding author

  
• G-actin regulates rapid induction of actin nucleation by mDia1 to restore cellular actin polymers

  Chiharu Higashida; Shiro Suetsugu; Takahiro Tsuji; James Monypenny; Shuh Narumiya; Naoki Watanabe

  JOURNAL OF CELL SCIENCE, Oct. 2008, Peer-reviewed, Last author, Corresponding author

  
• Shootin1 interacts with actin retrograde flow and L1-CAM to promote axon outgrowth

  Tadayuki Shimada; Michinori Toriyama; Kaori Uemura; Hiroyuki Kamiguchi; Tadao Sugiura; Naoki Watanabe; Naoyuki Inagaki

  JOURNAL OF CELL BIOLOGY, Jun. 2008, Peer-reviewed

  
• mDia2 induces the actin scaffold for the contractile ring and stabilizes its position during cytokinesis in NIH 3T3 cells

  Sadanori Watanabe; Yoshikazu Ando; Shingo Yasuda; Hiroshi Hosoya; Naoki Watanabe; Toshimasa Ishizaki; Shuh Narumiya

  MOLECULAR BIOLOGY OF THE CELL, May 2008, Peer-reviewed

  
• [Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen].

  Kinoshita M; Watanabe N

  Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, Oct. 2007, Invited

  
• [Actin polymerization machinery in live cells as revealed by single-molecule imaging].

  Tsuji T; Watanabe N

  Nihon rinsho. Japanese journal of clinical medicine, Feb. 2007, Invited

  
• Actin turnover-dependent fast dissociation of capping protein in the dendritic nucleation actin network: evidence of frequent filament severing

  Takushi Miyoshi; Takahiro Tsuji; Chiharu Higashida; Maud Hertzog; Akiko Fujita; Shuh Narumiya; Giorgio Scita; Naoki Watanabe

  JOURNAL OF CELL BIOLOGY, Dec. 2006, Peer-reviewed, Last author, Corresponding author

  
• [Actin dynamics].

  Watanabe N

  Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, May 2006, Invited

  
• [Probing actin biochemistry in living cells using single-molecule speckle microscopy].

  Higashida C; Miyoshi T; Watanabe N

  Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, Sep. 2005, Invited

  
• mDia1 and formins: Processive cappers surfing along with the fast-growing actin end

  N Watanabe; C Higashida

  SEIKAGAKU, Feb. 2005, Peer-reviewed, Invited

  
• [Actin polymerization kinetics in living cells disclosed by single-molecule speckle microscopy].

  Watanabe N

  Nihon yakurigaku zasshi. Folia pharmacologica Japonica, Feb. 2005, Peer-reviewed, Invited

  
• Formins: processive cappers of growing actin filaments

  N Watanabe; C Higashida

  EXPERIMENTAL CELL RESEARCH, Nov. 2004, Peer-reviewed, Invited, Lead author, Corresponding author

  
• Actin polymerization-driven molecular movement of mDia1 in living cells

  C Higashida; T Miyoshi; A Fujita; F OcegueraYanez; J Monypenny; Y Andou; S Narumiya; N Watanabe

  SCIENCE, Mar. 2004, Peer-reviewed, Last author, Corresponding author

  
• Single-molecule speckle analysis of Aactin filament turnover in lamellipodia

  N Watanabe; TJ Mitchison

  SCIENCE, Feb. 2002, Peer-reviewed, Lead author, Corresponding author

  
• Localization of a mammalian homolog of diaphanous, mDia1, to the mitotic spindle in HeLa cells

  T Kato; N Watanabe; Y Morishima; A Fujita; T Ishizaki; S Narumiya

  JOURNAL OF CELL SCIENCE, Feb. 2001, Peer-reviewed

  
• Rho-dependent transfer of Citron-kinase to the cleavage furrow of dividing cells

  M. Eda; S. Yonemura; T. Kato; N. Watanabe; T. Ishizaki; P. Madaule; S. Narumiya

  Journal of Cell Science, 2001

  
• Ropporin, a sperm-specific binding protein of rhophilin, that is localized in the fibrous sheath of sperm flagella

  A Fujita; K Nakamura; T Kato; N Watanabe; T Ishizaki; K Kimura; A Mizoguchi; S Narumiya

  JOURNAL OF CELL SCIENCE, Jan. 2000, Peer-reviewed

  
• Signaling from rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase

  M Maekawa; T Ishizaki; S Boku; N Watanabe; A Fujita; A Iwamatsu; T Obinata; K Ohashi; K Mizuno; S Narumiya

  SCIENCE, Aug. 1999, Peer-reviewed

  
• Cooperation between mDia1 and ROCK in Rho-induced actin reorganization

  N Watanabe; T Kato; A Fujita; T Ishizaki; S Narumiya

  NATURE CELL BIOLOGY, Jul. 1999, Peer-reviewed, Lead author

  
• Rhophilin, a small GTPase Rho-binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of the sperm tail

  K Nakamura; A Fujita; T Murata; G Watanabe; C Mori; J Fujita; N Watanabe; T Ishizaki; O Yoshida; S Narumiya

  FEBS LETTERS, Feb. 1999

  
• Role of citron kinase as a target of the small GTPase Rho in cytokinesis

  P Madaule; M Eda; N Watanabe; K Fujisawa; T Matsuoka; H Bito; T Ishizaki; S Narumiya

  NATURE, Jul. 1998, Peer-reviewed

  
• Molecular dissection of the Rho-associated protein kinase (p160ROCK)-regulated neurite remodeling in neuroblastoma N1E-115 cells

  M Hirose; T Ishizaki; N Watanabe; M Uehata; O Kranenburg; WH Moolenaar; F Matsumura; M Maekawa; H Bito; S Narumiya

  JOURNAL OF CELL BIOLOGY, Jun. 1998, Peer-reviewed

  
• Rho effectors and reorganization of actin cytoskeleton

  S Narumiya; T Ishizaki; N Watanabe

  FEBS LETTERS, Jun. 1997, Peer-reviewed

  
• p140mDia, a mammalian homolog of Drosophila diaphanous, is a target protein for Rho small GTPase and is a ligand for profilin

  N Watanabe; P Madaule; T Reid; T Ishizaki; G Watanabe; A Kakizuka; Y Saito; K Nakao; BM Jockusch; S Narumiya

  EMBO JOURNAL, Jun. 1997, Peer-reviewed, Lead author

  
• p160(ROCK), a Rho-associated coiled-coil forming protein kinase, works downstream of Rho and induces focal adhesions

  Toshimasa Ishizaki; Mamoru Naito; Kazuko Fujisawa; Midori Maekawa; Naoki Watanabe; Yuji Saito; Shuh Narumiya

  FEBS Letters, 10 Mar. 1997

  
• Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase, PKN, and rhophilin in the Rho-binding domain

  T Reid; T Furayashiki; T Ishizaki; G Watanabe; N Watanabe; K Fujisawa; N Morii; P Madaule; S Narumiya

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jun. 1996

  
• The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase

  T Ishizaki; M Maekawa; K Fujisawa; K Okawa; A Iwamatsu; A Fujita; N Watanabe; Y Saito; A Kakizuka; N Morii; S Narumiya

  EMBO JOURNAL, Apr. 1996

  
• Identification of Glu173 as the critical amino acid residue for the ADP-ribosyltransferase activity of Clostridium botulinum C3 exoenzyme

  Yuji Saito; Yasuo Nemoto; Toshimasa Ishizaki; Naoki Watanabe; Narito Morii; Shuh Narumiya

  FEBS Letters, 04 Sep. 1995

  
• Lysophosphatidic acid-induced activation of protein Ser/Thr kinases in cultured rat 3Y1 fibroblasts Possible involvement in rho p21-mediated signalling

  Naokazu Kumagai; Narito Morii; Toshimasa Ishizaki; Naoki Watanabe; Kazuko Fujisawa; Yuji Saito; Shuh Narumiya

  FEBS Letters, 05 Jun. 1995

  

Misc.

• Structural activation of target kinases and cancer cell proliferation induced by kinase inhibitors

  Naoki Watanabe

  生体の科学, 2023, Invited, Lead author, Last author, Corresponding author

  
• ミオシン張力によるアクチン線維安定化の細胞内蛍光単分子イメージング解析

  山城佐和子; 渡邊直樹

  BIO Clinica, Nov. 2022, Last author

  
• 細胞内蛍光単分子イメージングによるミオシン張力依存的なアクチン線維安定化の証明

  山城佐和子; 渡邊直樹

  生体の科学, Jun. 2021, Last author

  
• 単分子イメージングによる細胞内アクチン重合機構の解析

  渡邊 直樹; 三好 拓志; 東田 知陽

  バイオイメージング, 26 Sep. 2005

  
• アクチンネットワークを駆け巡る力学作用の分子可視化

  渡邊 直樹; 山城 佐和子

  生化学, Feb. 2020, Peer-reviewed, Lead author, Corresponding author

  
• 蛍光単分子イメージングが解き明かすミオシン張力によるアクチン線維安定化

  山城佐和子; 渡邊直樹

  月刊メディカル・サイエンス・ダイジェスト, 30 Jun. 2019

  
• 細胞構造と細胞質流動がつくるバイオロジー フォルミンファミリーの螺旋回転重合によるアクチン線維の構造変化と安定化

  渡邊 直樹

  日本生化学会大会プログラム・講演要旨集, Sep. 2018

  
• 細胞構造と細胞質流動がつくるバイオロジー ショウジョウバエ血球細胞が示す細胞質動態のキラリティ

  笹村 剛司; 栗栖 大祐; 秋山 正和; 大友 康平; 根本 知己; 水野 裕昭; 渡邊 直樹; 松野 健治

  日本生化学会大会プログラム・講演要旨集, Sep. 2018

  
• 生細胞内でのアクチンフィラメント配向の可視化

  通山 潔; 山城 佐和子; 渡邊 直樹

  日本生化学会大会プログラム・講演要旨集, Sep. 2018

  
• フォルミンとGアクチンホメオスターシスが織りなす多様なアクチン線維寿命と物理ストレス抵抗性

  渡邊直樹

  日本分子生物学会年会プログラム・要旨集(Web), 2018

  
• ショウジョウバエ血球細胞が示す細胞質動態のキラリティ

  笹村 剛司; 栗栖 大祐; 秋山 正和; 大友 康平; 根本 知己; 水野 裕昭; 渡邊 直樹; 松野 健治

  生命科学系学会合同年次大会, Dec. 2017

  
• 【細胞多様性解明に資する光技術-見て,動かす】見る,観る,視る 多重染色・高密度標識で従来法の壁を超えるIRIS超解像顕微鏡法

  渡邊 直樹; 木内 泰

  生体の科学, 15 Oct. 2017

  
• 細胞生物学とイメージング技術の共鳴誘導 高密度・多重染色超解像顕微鏡法IRISの3次元イメージングへの発展

  木内 泰; 渡邊 直樹

  日本細胞生物学会大会講演要旨集, May 2017

  
• 細胞のメカノセンスとシグナル伝達はいかに収束するか

  渡邊 直樹

  上原記念生命科学財団研究報告集, Dec. 2016

  
• 分子の窓を通して覗いた細胞の構造としくみ

  渡邊直樹; 渡邊直樹; 木内泰; 山城佐和子

  バイオイメージング, 22 Aug. 2016

  
• 分子から個体まで神経系を見る 分子の窓を通して覗いた細胞の構造としくみ

  渡邊 直樹; 木内 泰; 山城 佐和子

  バイオイメージング, Aug. 2016

  
• 超解像顕微鏡法IRISの開発による無制限多重染色と高精細な画質の実現

  木内泰; 渡邊直樹; 渡邊直樹

  感染・炎症・免疫, 10 Jul. 2016

  
• 実験講座 高密度・多重染色超解像蛍光顕微鏡法IRISの原理と実践

  木内 泰; 渡邊 直樹

  生体の科学, Jun. 2016

  
• 細胞生物学に役に立つ最新技術の展開 多重高密度標識超解像顕微鏡IRISとその応用

  渡邊 直樹; 三好 拓志; 三宅 崇文; 佐々木 瞭; 木内 泰

  日本細胞生物学会大会講演要旨集, May 2016

  
• ナノスケール単分子スペックル解析により明らかにする細胞仮足が「つかみ取る」しくみ

  山城 佐和子; 田中 聡一郎; 渡邊 直樹

  日本細胞生物学会大会(Web), 2016

  
• 【細胞シグナル操作法】機能からみたシグナル操作法 細胞の動態 細胞骨格(アクチン系)

  山城 佐和子; 渡邊 直樹

  生体の科学, 15 Oct. 2015

  
• リーディングエッジでの外力センサーとしてのアクチン重合の役割(The role of actin polymerization as an external force sensor at the leading edge)

  小関 和馬; 渡邊 直樹

  日本生化学会大会プログラム・講演要旨集, Oct. 2014

  
• 【生命動態システム科学】 定量生物学 分子・細胞の計測 1分子計測 単分子スペックル(SiMS)顕微鏡が解明したアクチン重合制御

  小関 和馬; 渡邊 直樹

  生体の科学, Oct. 2014

  
• 分子イメージングはどこまで進んだか II 分子イメージングの最新動向 3.光イメージングの最新動向 2)細胞内蛍光単分子イメージングが解き明かす生命の動的なしくみ

  渡邊直樹; 山城佐和子; 水野裕昭

  Innervision, 25 Jun. 2014

  
• 【Molecular Imaging 2014 分子イメージングはどこまで進んだか】 分子イメージングの最新動向 光イメージングの最新動向 細胞内蛍光単分子イメージングが解き明かす生命の動的なしくみ

  渡邊 直樹; 山城 佐和子; 水野 裕昭

  INNERVISION, Jun. 2014

  
• Timothy J. Mitchison 細胞骨格研究の偉大なジーク

  渡邊 直樹

  実験医学, Mar. 2014

  
• The role of actin polymerization as an external force sensor at the leading edge

  小関和馬; 渡邊直樹

  日本生化学会大会(Web), 2014

  
• タンパク質構造機能相関再考 アクチン線維への物理ストレスに対抗するアクチンホメオスターシスとフォルミンファミリーによる急速線維回生機構

  渡邊直樹

  生化学, 25 Aug. 2013

  
• 【細胞骨格イメージング】 蛍光単分子可視化と他の分子動態解析法の融合による細胞内アクチン重合機構の解明

  渡邊 直樹; 木内 泰

  顕微鏡, Aug. 2013

  
• Update Review アクチン研究の最新動向 構造から調節、恒常性、可視化、モデリングまで

  山城 佐和子; 圓岡 真宏; 水野 裕昭; 渡邊 直樹

  実験医学, Nov. 2012

  
• Inhibitor induced conformational modulation of target kinases

  Naoki Watanabe; Yunfeng Yuan; Masahiro Maruoka

  JOURNAL OF PHARMACOLOGICAL SCIENCES, 2012

  
• フォルミンmDia1の螺旋回転を介したアクチン脱重合抵抗性

  水野裕昭; 山城佐和子; 渡邊直樹

  日本分子生物学会年会プログラム・要旨集(Web), 2012

  
• An Autocatalytic Activator-Inhibitor Model of Actin Polymerization at the Leading Edge of Fibroblast Lamellipodia

  Gillian L. Ryan; Matthew B. Smith; Heather Petroccia; Naoki Watanabe; Dimitrios Vavylonis

  BIOPHYSICAL JOURNAL, Feb. 2011

  
• フォルミンファミリーを介した細胞の機械受容とアクチン動態の連結

  渡邊直樹

  生化学, 2011

  
• mDia1は回転運動しながらプロセッシブにアクチンを重合する

  水野裕昭; 渡邊直樹

  生化学, 2011

  
• The Role of Rotational Movement of a Mammalian Formin mDia1 in Actin Remodeling

  水野裕昭; 渡邊直樹

  生物物理, 2011

  
• Development of a strategy to screen small compounds that modulate Abl kinase activity through conformational regulation

  Yunfeng Yuan; Shuh Narumiya; Naoki Watanabe

  JOURNAL OF PHARMACOLOGICAL SCIENCES, 2010

  
• プロセッシブなアクチン重合の間に起こるmDia1の回転運動の一分子可視化

  水野裕昭; 渡邊直樹

  生化学, 2010

  
• Bcr‐AblによるVASPのチロシンリン酸化は白血病細胞における接着能の亢進に関与する

  圓岡真宏; YUAN Yunfeng; 渡邊直樹

  生化学, 2010

  
• Rho標的蛋白質mDia結合蛋白質Liprin‐αよるアクチン細胞骨格の制御メカニズムの解明

  坂本智子; 石崎敏理; 渡邊直樹; 大川克也; 成宮周

  生化学, 2010

  
• mDiaからプロセッシブに伸長するアクチン線維の回転運動の1分子可視化

  水野裕昭; 東田知陽; 成宮周; 渡邊直樹

  生化学, 25 Sep. 2009

  
• ST1571-induced cell edge translocation of abl

  Yunfeng Yuan; Akiko Fujita; Tomoyuki Shishido; Shuh Narumiya; Naoki Watanabe

  JOURNAL OF PHARMACOLOGICAL SCIENCES, 2009

  
• A ROLE OF HOMEOSTASIS BETWEEN G- AND F-ACTIN IN CELLULAR ACTIN POLYMER RESTORATION MECHANISMS INVOLVING A Rho EFFECTER, mDia1

  Naoki Watanabe; Chiharu Higashida; Hiroaki Mizuno

  JOURNAL OF PHYSIOLOGICAL SCIENCES, 2009

  
• 細胞という名の小宇宙

  渡邊 直樹

  細胞生物, Apr. 2008

  
• Rapid activation of mDia1 to restore cellular actin polymers regulated by G-actin

  Chiharu Higashida; Shiro Suetsugu; Shuh Narumiya; Naoki Watanabe

  JOURNAL OF PHARMACOLOGICAL SCIENCES, 2008

  
• 単分子イメージングが明らかにした生細胞内アクチン重合機構

  辻貴宏; 渡邊直樹

  日本臨床, 01 Feb. 2007

  
• Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen (ケミカルバイオロジー) -- (代表的論文からケミカルバイオロジーの歴史をさかのぼる 生理活性物質のケミカルバイオロジーを開拓した論文)

  木下 専; 渡邊直樹

  蛋白質核酸酵素, 2007, Invited

  
• 新規タンパク質shootin1は,F‐actinの求心的移動とL1‐CAMを連結することで神経突起伸長を促進する

  島田忠之; 鳥山道則; 上口裕之; 杉浦忠男; 渡邊直樹; 稲垣直之

  生化学, 2007

  
• 新たな研究ストラテジー 4.単分子スペックル顕微鏡によるアクチンダイナミックス観察

  渡邊直樹

  実験医学, 15 Aug. 2006

  
• 細胞の形の制御 7.アクチン細胞骨格とアクチン調節タンパク質

  馬渕一誠; 渡邊直樹; 寺崎朝子; 高稲正勝; 斧正一郎

  実験医学, 15 Aug. 2006

  
• Rapid Activation of mDia1 to Restore Cellular Actin Polymers Regulated by G-actin

  C. Higashida; S. Kobayashi; J. Monypenny; S. Narumiya; N. Watanabe

  MOLECULAR BIOLOGY OF THE CELL, 2006

  
• Actin Dynamics-dependent Fast Actin Uncapping in Dendritic Nucleation Network

  T. Miyoshi; T. Tsuji; N. Watanabe

  MOLECULAR BIOLOGY OF THE CELL, 2006

  
• がん研究に係わる特定領域研究研究報告集録 がん特性(がんにおける細胞・組織システムの破綻)がん細胞の接着・運動 癌細胞浸潤の細胞進展縁アクチンの求心性流動変化による制御

  渡邊直樹

  がん研究に係わる特定領域研究研究報告集録 平成17年度, 2006

  
• Evidence of constitutive actin filament severing in lamellipodia

  Takushi Miyoshi; Chiharu Higashida; Naoki Watanabe

  CELL STRUCTURE AND FUNCTION, Jun. 2005

  
• アクチン重合駆動モーター"Forminファミリー"

  渡邊 直樹; 東田 知陽

  化学と工業 = Chemistry and chemical industry, 01 Feb. 2005

  
• Molecular kinetics of the Arp 2/3 complex and capping proteins at the leading edge of living cells as revealed by single-molecule speckle microscopy

  T Miyoshi; C Higashida; N Watanabe

  MOLECULAR BIOLOGY OF THE CELL, Nov. 2004

  
• Actin polymerization-driven molecular movement of mDia1 in living cells

  C Higashida; T Miyoshi; A Fujita; F Oceguera-Yanez; J Monypenny; Y Andou; S Narumiya; N Watanabe

  FASEB JOURNAL, May 2004

  
• Actin polymerization-driven directional movement of mDial as revealed by single-molecule speckle microscopy in living cells

  Chiharu Higashida; Shuh Narumiya; Naoki Watanabe

  CELL STRUCTURE AND FUNCTION, May 2004

  
• Most of the actin filaments in lamellipodia are generated by polymerization away from the lamellipodium tip

  N Watanabe; TJ Mitchison

  MOLECULAR BIOLOGY OF THE CELL, Nov. 2001

  
• RhoエフェクターmDia1の紡錘体への局在

  加藤隆幸; 石崎敏理; 渡辺直樹; 成宮周

  日本細胞生物学会大会講演要旨集, 2001

  
• RhoエフェクターmDia1の紡錘体への局在

  加藤隆幸; 渡辺直樹; 石崎敏理; 成宮周

  生化学, 25 Aug. 2000

  
• RhoエフェクターmDia1の紡錘体への局在

  加藤隆幸; 石崎敏理; 渡辺直樹; 成宮周

  日本分子生物学会年会プログラム・講演要旨集, 22 Nov. 1999

  
• 低分子量G蛋白質Rhoによるアクチン細胞骨格系の制御

  前川みどり; 石崎敏理; 渡辺直樹; 大橋一正; 水野健作; 成宮周

  日本分子生物学会年会プログラム・講演要旨集, 22 Nov. 1999

  
• Effectors of Rho family of GTPases: Recent progress.

  渡辺直樹; 石崎敏理

  実験医学, 01 May 1999

  
• Rhoに誘導されるアクチンストレス線維形成におけるmDiaとROCKの相互作用

  渡辺直樹; 加藤隆幸; 石崎敏理; 成宮周

  日本分子生物学会年会プログラム・講演要旨集, Nov. 1998

  
• Rhoに誘導されるアクチンストレス線維形成におけるmDiaとROCKの相互作用

  渡辺直樹; 加藤隆幸; 石崎敏理; 成宮周

  日本分子生物学会年会プログラム・講演要旨集, Nov. 1998

  
• Rho effectors―cellular functions to pathophysiological roles.

  成宮周; 石崎敏理; 渡辺直樹

  実験医学, Sep. 1998

  

Books and Other Publications

• 阻害剤・活性化剤ハンドブック

  渡邊 直樹, Joint work, 第32章 細胞骨格・細胞分裂関連薬剤① アクチン細胞骨格系

  羊土社, 2019, Not refereed

  
• ラング・デール薬理学

  Rang H. P; Ritter James M; Flower R. J; Henderson G; 渡邊, Others

  エルゼビア・ジャパン, 丸善出版 (発売), 2018, Not refereed

  Find Kyoto University's collection of books :

  
• 京大発！フロンティア生命科学

  渡邊 直樹, Joint work, 細胞骨格～動的な形態制御のメカニズム 4章 4.2節

  2018, Not refereed

  
• Experimental Medicine

  WATANABE Naoki, Joint work, Timothy J. Mitchison 細胞骨格研究の偉大なジーク

  Yodosha, 2014, Not refereed

  
• A possible role of homeostasis between monomeric and filamentous actin in filament nucleation revealed by pharmacokinetic modeling.

  WATANABE Naoki; HIGASHIDA Chiharu, Joint work, Systems Biology The Challenge of Complexity

  Springer, 2009, Not refereed

  
• アクチン重合に駆動される分子移動装置フォルミン蛋白質の生細胞内動態

  渡邊 直樹; 京都大学大学院医学研究科

  [渡邊直樹], 2007, Not refereed

  
• 細胞の中を分子でのぞく－Less is moreとSerendipity－

  渡邊 直樹, Joint work, 巻頭言

  学術月報 第58巻 第5号 389－391, Not refereed

  

Awards

• 2004

  第１回日本学術振興会賞JSPS Prize

Media Coverage

• キナーゼ阻害薬は諸刃の剣か？抵抗性変異との遭遇で細胞を増殖 京大グループがc-Srcシグナルの逆説的活性化を報告

  日経バイオテクONLINE, 日経バイオテク, May 2021, Internet

  
• 細胞内たんぱく質 さらに精密に観察

  朝日新聞, 23面, 13 Aug. 2015

  
• 細胞骨格 くっきり撮影

  京都新聞, 夕刊1面, 27 Jul. 2015

  
• 京大、同時に複数のたんぱく質を染色し高精細な画像撮影できる超解像顕微鏡法を開発

  日刊工業新聞, 07 Jul. 2015

  

External funds: Kakenhi

• Elucidation of mechanotransduction mechanisms with visualization of molecules

  Grant-in-Aid for Scientific Research (A)

  Medium-sized Section 48:Biomedical structure and function and related fields

  Kyoto University

  Naoki Watanabe

  From 01 Apr. 2019, To 31 Mar. 2022, Project Closed

  メカノトランスダクション;アクチン重合;ブラウンラチェット;動的不安定性;超解像可視化用プローブ;がん治療キナーゼ阻害薬;逆説的標的分子活性化;アロステリック作用;細胞接着;アクチン流動;単分子イメージング;ブラウンラチェット仮説;キナーゼ阻害薬;超解像顕微鏡;蛍光単分子イメージング;フォルミンファミリー;アクチン重合センサー;分子標的薬;Src;力覚センサー;細胞運動;接着斑;アクチン;物理ストレス

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H01020/
• アクチン単量体-線維のホメオスターシスを司る細胞シグナルによる物理ストレス応答

  Grant-in-Aid for Scientific Research (B)

  Tohoku University;Kyoto University

  渡邊 直樹

  From 01 Apr. 2009, To 31 Mar. 2011, Project Closed

  細胞・組織;一分子計測;生体分子;ナノバイオ;G蛋白質;mDia;細胞シグナル;フォルミンファミリー

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390078/
• SDF-1 plays a role in bone growth and repair by cytoskeleton modulation

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Hiromu ITO

  From 01 Apr. 2007, To 31 Mar. 2009, Project Closed

  骨折;成長軟骨;ケモカイン;細胞内骨格

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19591756/
• Elucidation of acute actin polymerization signaling using single molecule imaging of filament end factors

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Naoki WATANABE

  From 01 Apr. 2007, To 31 Mar. 2009, Project Closed

  細胞;組織;一分子計測;生体分子;ナノバイオ;細胞・組織;コフィリン;アクチン重合;細胞シグナル;フォルミンファミリー;アクチン線維切断

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390075/
• Actin-polymerization-driven molecular dynamics of Formin homology proteins in live cells

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Naoki WATANABE

  From 01 Apr. 2005, To 31 Mar. 2007, Project Closed

  フォルミン蛋白質;mDial;Rho;一分子計測;アクチン重合;プロセッシブキャップ;恒常性;単量体アクチン;細胞・組織;生体分子;ナノバイオ;mDia;細胞シグナル;フォルミンファミリー, formin family;mDia1;Rho;single-molecule imaging;actin polymerization;processive capping;homeostasis;globular actin

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390077/
• Single-molecule imaging analysis of cell structural reorganization processes downstream of G protein signaling

  Grant-in-Aid for Scientific Research on Priority Areas

  Biological Sciences

  Kyoto University

  Naoki WATANABE

  From 01 Apr. 2005, To 31 Mar. 2010, Project Closed

  細胞・組織;一分子計測;G蛋白質;フォルミンファミリー;Rhoファミリー;生体分子;mDia;細胞シグナル;ナノバイオ;アクチン線維切断;恒常性

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17079004/
• アクチン重合端振動の細胞内可視化

  Grant-in-Aid for Scientific Research on Priority Areas

  Biological Sciences

  Kyoto University

  渡邊 直樹

  From 01 Apr. 2005, To 31 Mar. 2007, Project Closed

  細胞・組織;一分子計測;生体分子;ナノバイオ;アクチン重合;キャッピングプロテイン;細胞シグナル;細胞運動

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17049018/
• 癌細胞浸潤の細胞伸展縁アクチンの求心性流動変化による制御

  Grant-in-Aid for Scientific Research on Priority Areas

  Biological Sciences

  Kyoto University

  渡邊 直樹

  From 01 Apr. 2005, To 31 Mar. 2006, Project Closed

  細胞・組織;一分子計測;生体分子;癌細胞浸潤;アクチン重合;分子モーター;細胞シグナル;クラッチモデル

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17014050/
• 悪性神経膠芽腫におけるRho情報伝達系による浸潤機構の解析と創薬の探索

  Grant-in-Aid for Exploratory Research

  Kyoto University

  橋本 信夫

  From 01 Apr. 2004, To 31 Mar. 2006, Project Closed

  Rbo;mDia1;神経膠芽腫;接着班;SDF-1α;浸潤;Rho;mDial

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16659386/
• 癌細胞浸潤における細胞内アクチンの求心性流動の役割

  Grant-in-Aid for Scientific Research on Priority Areas

  Biological Sciences

  Kyoto University

  渡邊 直樹

  From 01 Apr. 2004, To 31 Mar. 2005, Project Closed

  レトログレードフロー;葉状仮足;細胞伸展;アクチン重合;Formin;mDia1;Rho;ミオシン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16022234/
• Identification of intracellular signaling cascade of Rho and mDia1 based on high sensitivity live-cell imaging

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Naoki WATANABE

  From 01 Apr. 2003, To 31 Mar. 2005, Project Closed

  Forminファミリー;mDial;Rho;単分子イメージング;アクチン重合;分子モニター;プロフィリン;プロセッシブキャッピング;分子モーター;mDia1;1分子イメージング;モーター分子;Formin;スペックル顕微鏡, Formin family;mDia1;Rho;single-molecule imaging;actin polymerization;molecular motor;profilin;processive capping

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15370086/
• 癌細胞浸潤における細胞内アクチンの求心性流動の役割

  Grant-in-Aid for Scientific Research on Priority Areas

  Biological Sciences

  Kyoto University

  渡邊 直樹

  From 01 Apr. 2003, To 31 Mar. 2004, Project Closed

  アクチン求心性流動;アクチン重合;ミオシン;mDia1;Formin;ARP2;3複合体;細胞伸展;葉状仮足

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15024239/
• 分子・構造変換のリアルタイム可視化からの病気の解明と治療戦略

  Grant-in-Aid for Scientific Research (A)

  Medium-sized Section 50:Oncology and related fields

  Kyoto University

  渡邊 直樹

  From 01 Apr. 2022, To 31 Mar. 2025, Granted

  キナーゼ阻害薬;アロステリック効果;逆説的活性化;細胞分子イメージング;超解像顕微鏡;多重細胞マーカー検出;抗体改変技術;アクチンオリゴマー

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H00456/

External funds: others

• 多重高密度超解像顕微鏡IRISによる多分子複合体マッピング

  統合1 細胞解析のための ⾰新的技術基盤

  JST, CREST

  From 01 Oct. 2015, To 31 Mar. 2021

  渡邊直樹

  研究代表者
• Comprehensive Analysis of Cell Motility Machinary using the Single Molecule Speckle Method.

  さきがけ

  From 2002, To 31 Mar. 2006

  渡邊直樹
• Elucidation of mechanosense cell signaling by fluorescence single-molecule imaging of actin polymerizing machinery

  最先端次世代研究支援プログラム

  From Feb. 2011, To Mar. 2014

  渡邊　直樹

Education

Teaching subject(s)

• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Course in Immunology and Human Disorders

  1031, Fall, Graduate School of Biostudies, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Cell, Developmental and Systems Biologys (Practice for Master Program)

  E055, Year-long, Graduate School of Medicine, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Pharmacology I

  E015, Spring, Graduate School of Medicine, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Pharmacology

  M009, Spring, Graduate School of Medicine, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Cell, Developmental and Systems Biology (Practice)

  P030, Year-long, Graduate School of Medicine, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Experiments in Systemic Life Sciences

  5013, Spring, Graduate School of Biostudies, 4
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Experiments in Systemic Life Sciences

  5013, Fall, Graduate School of Biostudies, 4
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course and Seminar D

  2009, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course and Seminar C

  2008, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course and Seminar C

  2008, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course and Seminar D

  2009, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course and Seminar A

  2006, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course and Seminar B

  2007, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course and Seminar B

  2007, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course and Seminar A

  2006, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2024, To 31 Mar. 2025

  Pharmacology II

  E016, Spring, Graduate School of Medicine, 4
• From 01 Apr. 2024, To 31 Mar. 2025

  Cell, Developmental and Systems Biology (Seminar)

  P029, Year-long, Graduate School of Medicine, 4
• From 01 Apr. 2024, To 31 Mar. 2025

  Cell, Developmental and Systems Biology (Seminar for Master Program)

  E054, Year-long, Graduate School of Medicine, 4
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Molecular and Cell Biology I

  1016, Spring, Graduate School of Biostudies, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Systems Biology

  8213, Year-long, Graduate School of Pharmaceutical Sciences, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Advanced Course in Immunology and Human Disorders

  1031, Spring, Graduate School of Biostudies, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Cell, Developmental and Systems Biology (Practice)

  P030, Year-long, Graduate School of Medicine, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Pharmacology

  M009, Spring, Graduate School of Medicine, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Cell, Developmental and Systems Biologys (Practice for Master Program)

  E055, Year-long, Graduate School of Medicine, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Pharmacology I

  E015, Spring, Graduate School of Medicine, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Experimental Course and Seminar A

  2006, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2023, To 31 Mar. 2024

  Experimental Course and Seminar D

  2009, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2023, To 31 Mar. 2024

  Experimental Course and Seminar B

  2007, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2023, To 31 Mar. 2024

  Experimental Course and Seminar B

  2007, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2023, To 31 Mar. 2024

  Experimental Course and Seminar A

  2006, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2023, To 31 Mar. 2024

  Experimental Course and Seminar D

  2009, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2023, To 31 Mar. 2024

  Experimental Course and Seminar C

  2008, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2023, To 31 Mar. 2024

  Experimental Course and Seminar C

  2008, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2023, To 31 Mar. 2024

  Cell, Developmental and Systems Biology (Seminar)

  P029, Year-long, Graduate School of Medicine, 4
• From 01 Apr. 2023, To 31 Mar. 2024

  Cell, Developmental and Systems Biology (Seminar for Master Program)

  E054, Year-long, Graduate School of Medicine, 4
• From 01 Apr. 2023, To 31 Mar. 2024

  Pharmacology II

  E016, Spring, Graduate School of Medicine, 4
• From 01 Apr. 2023, To 31 Mar. 2024

  Advanced Experiments in Systemic Life Sciences

  5013, Spring, Graduate School of Biostudies, 4
• From 01 Apr. 2023, To 31 Mar. 2024

  Advanced Experiments in Systemic Life Sciences

  5013, Fall, Graduate School of Biostudies, 4
• From 01 Apr. 2023, To 31 Mar. 2024

  Advanced Molecular and Cell Biology I

  1016, Spring, Graduate School of Biostudies, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Pharmacology Laboratory Class and Tutorial

  B017, Spring, Faculty of Medicine, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar B

  2007, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Molecular and Cell Biology I

  1016, Spring, Graduate School of Biostudies, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar A

  2006, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Experiments in Systemic Life Sciences

  5013, Spring, Graduate School of Biostudies, 4
• From 01 Apr. 2022, To 31 Mar. 2023

  Pharmacology II

  E016, Spring, Graduate School of Medicine, 4
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar D

  2009, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar C

  2008, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Pharmacology

  M009, Spring, Graduate School of Medicine, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar D

  2009, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar A

  2006, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar C

  2008, Spring, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Experiments in Systemic Life Sciences

  5013, Fall, Graduate School of Biostudies, 4
• From 01 Apr. 2022, To 31 Mar. 2023

  Pharmacology I

  E015, Spring, Graduate School of Medicine, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Laboratory Course in Pharmacology

  L190, Fall, Faculty of Medicine, 0
• From 01 Apr. 2022, To 31 Mar. 2023

  Pharmacology

  L180, Fall, Faculty of Medicine, 0
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course and Seminar B

  2007, Fall, Graduate School of Biostudies, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Course in Immunology and Human Disorders

  1031, Spring, Graduate School of Biostudies, 1
• From Apr. 2014, To Mar. 2015

  Pharmacology II

  Fall, 医学研究科
• From Apr. 2014, To Mar. 2015

  Pharmacology I

  Fall, 医学研究科
• From Apr. 2014, To Mar. 2015

  Pharmacology

  Fall, 医学研究科
• From Apr. 2015, To Mar. 2016

  Pharmacology

  Fall, 医学部
• From Apr. 2015, To Mar. 2016

  Laboratory Course in Pharmacology

  Fall, 医学部
• From Apr. 2015, To Mar. 2016

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2015, To Mar. 2016

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2015, To Mar. 2016

  Advanced Course in Immunology and Human Disorders

  Fall, 生命科学研究科
• From Apr. 2015, To Mar. 2016

  Pharmacology II

  Fall, 医学研究科
• From Apr. 2015, To Mar. 2016

  Pharmacology I

  Fall, 医学研究科
• From Apr. 2015, To Mar. 2016

  Pharmacology

  Fall, 医学研究科
• From Apr. 2015, To Mar. 2016

  Advanced Experiments in Systemic Life Sciences

  Spring, 生命科学研究科
• From Apr. 2015, To Mar. 2016

  Advanced Experiments in Systemic Life Sciences

  Fall, 生命科学研究科
• From Apr. 2016, To Mar. 2017

  Pharmacology

  Fall, 医学部
• From Apr. 2016, To Mar. 2017

  Laboratory Course in Pharmacology

  Fall, 医学部
• From Apr. 2016, To Mar. 2017

  Laboratory visit

  Spring, 医学研究科
• From Apr. 2016, To Mar. 2017

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2016, To Mar. 2017

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2016, To Mar. 2017

  Advanced Course in Immunology and Human Disorders

  Spring, 生命科学研究科
• From Apr. 2016, To Mar. 2017

  Pharmacology II

  Fall, 医学研究科
• From Apr. 2016, To Mar. 2017

  Pharmacology I

  Fall, 医学研究科
• From Apr. 2016, To Mar. 2017

  Pharmacology

  Fall, 医学研究科
• From Apr. 2016, To Mar. 2017

  Advanced Experiments in Systemic Life Sciences

  Spring, 生命科学研究科
• From Apr. 2016, To Mar. 2017

  Advanced Experiments in Systemic Life Sciences

  Fall, 生命科学研究科
• From Apr. 2017, To Mar. 2018

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2017, To Mar. 2018

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2017, To Mar. 2018

  Advanced Course in Immunology and Human Disorders

  Fall, 生命科学研究科
• From Apr. 2017, To Mar. 2018

  Advanced Experiments in Systemic Life Sciences

  Spring, 生命科学研究科
• From Apr. 2017, To Mar. 2018

  Advanced Experiments in Systemic Life Sciences

  Fall, 生命科学研究科
• From Apr. 2018, To Mar. 2019

  Pharmacology

  Spring, 医学部
• From Apr. 2018, To Mar. 2019

  Pharmacology Laboratory Class and Tutorial

  Spring, 医学部
• From Apr. 2018, To Mar. 2019

  Pharmacology

  Fall, 医学部
• From Apr. 2018, To Mar. 2019

  Laboratory Course in Pharmacology

  Fall, 医学部
• From Apr. 2018, To Mar. 2019

  Frontier in Life Sciences

  Fall, 全学共通科目
• From Apr. 2018, To Mar. 2019

  Frontier in Life Sciences

  Fall, 生命科学研究科
• From Apr. 2018, To Mar. 2019

  Experimental Course and Seminar A

  Spring, 生命科学研究科
• From Apr. 2018, To Mar. 2019

  Experimental Course and Seminar A

  Fall, 生命科学研究科
• From Apr. 2018, To Mar. 2019

  Experimental Course and Seminar B

  Spring, 生命科学研究科
• From Apr. 2018, To Mar. 2019

  Experimental Course and Seminar B

  Fall, 生命科学研究科
• From Apr. 2018, To Mar. 2019

  Experimental Course and Seminar C

  Spring, 生命科学研究科
• From Apr. 2018, To Mar. 2019

  Experimental Course and Seminar C

  Fall, 生命科学研究科
• From Apr. 2018, To Mar. 2019

  Experimental Course and Seminar D

  Spring, 生命科学研究科
• From Apr. 2018, To Mar. 2019

  Experimental Course and Seminar D

  Fall, 生命科学研究科
• From Apr. 2018, To Mar. 2019

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2018, To Mar. 2019

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2018, To Mar. 2019

  Advanced Course in Immunology and Human Disorders

  Spring, 生命科学研究科
• From Apr. 2018, To Mar. 2019

  Pharmacology II

  Spring, 医学研究科
• From Apr. 2018, To Mar. 2019

  Pharmacology I

  Spring, 医学研究科
• From Apr. 2018, To Mar. 2019

  Pharmacology

  Spring, 医学研究科
• From Apr. 2018, To Mar. 2019

  Advanced Experiments in Systemic Life Sciences

  Spring, 生命科学研究科
• From Apr. 2018, To Mar. 2019

  Advanced Experiments in Systemic Life Sciences

  Fall, 生命科学研究科
• From Apr. 2019, To Mar. 2020

  Pharmacology

  Spring, 医学部
• From Apr. 2019, To Mar. 2020

  Pharmacology Laboratory Class and Tutorial

  Spring, 医学部
• From Apr. 2019, To Mar. 2020

  Pharmacology

  Fall, 医学部
• From Apr. 2019, To Mar. 2020

  Laboratory Course in Pharmacology

  Fall, 医学部
• From Apr. 2019, To Mar. 2020

  Frontier in Life Sciences

  Fall, 全学共通科目
• From Apr. 2019, To Mar. 2020

  Frontier in Life Sciences

  Fall, 生命科学研究科
• From Apr. 2019, To Mar. 2020

  Experimental Course and Seminar A

  Spring, 生命科学研究科
• From Apr. 2019, To Mar. 2020

  Experimental Course and Seminar A

  Fall, 生命科学研究科
• From Apr. 2019, To Mar. 2020

  Experimental Course and Seminar B

  Spring, 生命科学研究科
• From Apr. 2019, To Mar. 2020

  Experimental Course and Seminar B

  Fall, 生命科学研究科
• From Apr. 2019, To Mar. 2020

  Experimental Course and Seminar C

  Spring, 生命科学研究科
• From Apr. 2019, To Mar. 2020

  Experimental Course and Seminar C

  Fall, 生命科学研究科
• From Apr. 2019, To Mar. 2020

  Experimental Course and Seminar D

  Spring, 生命科学研究科
• From Apr. 2019, To Mar. 2020

  Experimental Course and Seminar D

  Fall, 生命科学研究科
• From Apr. 2019, To Mar. 2020

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2019, To Mar. 2020

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2019, To Mar. 2020

  Advanced Course in Immunology and Human Disorders

  Spring, 生命科学研究科
• From Apr. 2019, To Mar. 2020

  Advanced Experiments in Systemic Life Sciences

  Spring, 生命科学研究科
• From Apr. 2019, To Mar. 2020

  Advanced Experiments in Systemic Life Sciences

  Fall, 生命科学研究科
• From Apr. 2020, To Mar. 2021

  Pharmacology

  Spring, 医学部
• From Apr. 2020, To Mar. 2021

  Pharmacology Laboratory Class and Tutorial

  Spring, 医学部
• From Apr. 2020, To Mar. 2021

  Pharmacology

  Fall, 医学部
• From Apr. 2020, To Mar. 2021

  Laboratory Course in Pharmacology

  Fall, 医学部
• From Apr. 2020, To Mar. 2021

  Frontier in Life Sciences

  Fall, 全学共通科目
• From Apr. 2020, To Mar. 2021

  Frontier in Life Sciences

  Fall, 生命科学研究科
• From Apr. 2020, To Mar. 2021

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2020, To Mar. 2021

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2020, To Mar. 2021

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2020, To Mar. 2021

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2020, To Mar. 2021

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2020, To Mar. 2021

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2020, To Mar. 2021

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2020, To Mar. 2021

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2020, To Mar. 2021

  Advanced Experiments in Systemic Life Sciences

  Spring, 生命科学研究科
• From Apr. 2020, To Mar. 2021

  Advanced Experiments in Systemic Life Sciences

  Fall, 生命科学研究科
• From Apr. 2020, To Mar. 2021

  Advanced Course in Immunology and Human Disorders

  Fall, 生命科学研究科
• From Apr. 2020, To Mar. 2021

  Pharmacology II

  Spring, 医学研究科
• From Apr. 2020, To Mar. 2021

  Pharmacology I

  Spring, 医学研究科
• From Apr. 2020, To Mar. 2021

  Pharmacology

  Spring, 医学研究科
• From Apr. 2021, To Mar. 2022

  Advanced Molecular and Cell Biology I

  Spring, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Pharmacology

  Fall, 医学部
• From Apr. 2021, To Mar. 2022

  Laboratory Course in Pharmacology

  Fall, 医学部
• From Apr. 2021, To Mar. 2022

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course and Seminar

  Spring, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course and Seminar

  Fall, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Advanced Experiments in Systemic Life Sciences

  Spring, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Advanced Experiments in Systemic Life Sciences

  Fall, 生命科学研究科
• From Apr. 2021, To Mar. 2022

  Introduction to Biology and Life Science

  Spring, 全学共通科目
• From Apr. 2021, To Mar. 2022

  Pharmacology II

  Spring, 医学研究科
• From Apr. 2021, To Mar. 2022

  Pharmacology I

  Spring, 医学研究科
• From Apr. 2021, To Mar. 2022

  Pharmacology

  Spring, 医学研究科

Participation in PhD Defense

• Chemoproteomic Identification of Blue-Light-Damaged Proteins

  TOH KOHEI, Graduate School of Medicine, Sub-chief Examiner

  25 Mar. 2024
• Involvement of endothelins in neuroprotection of valosin-containing protein modulators against retinal ganglion cell damage

  KUSAKA MAMI, Graduate School of Medicine, Sub-chief Examiner

  25 Mar. 2024
• A novel cell-based assay for the high-throughput screening of epithelial-mesenchymal transition inhibitors: Identification of approved and investigational drugs that inhibit epithelial-mesenchymal transition

  ISHIKAWA HIROYUKI, Graduate School of Medicine, Sub-chief Examiner

  25 Sep. 2023
• The molecular associations in clathrin-coated pit regulate β-arrestin-mediated MAPK signaling downstream of μ-opioid receptor

  SATO ATSUKO, Graduate School of Medicine, Chief Examiner

  23 Mar. 2023
• Trapping of CDC42 C-terminal variants in the Golgi drives pyrin inflammasome hyperactivation

  ISA MASAHIKO, Graduate School of Medicine, Sub-chief Examiner

  23 Mar. 2023
• Dual inhibition of the MEK/ERK and PI3K/AKT pathways prevents pulmonary GVHD suppressing perivenulitis and bronchiolitis

  MURANUSHI HIROYUKI, Graduate School of Medicine, Sub-chief Examiner

  23 Mar. 2023
• Conformational change of RNA‐helicase DHX30 by ALS/FTD‐linked FUS induces mitochondrial dysfunction and cytosolic aggregates

  HIKIAMI RYOTA, Graduate School of Medicine, Sub-chief Examiner

  23 Jan. 2023
• Discovery of a Phase-Separating Small Molecule That Selectively Sequesters Tubulin in Cells

  GENYIR ADO, Graduate School of Medicine, Chief Examiner

  23 Jan. 2023
• Development of fast-dissociating recombinant antibodies for high-density multiplexed IRIS super-resolution microscopy

  ZHANG QIANLI, Graduate School of Biostudies, Chief Examiner

  24 Nov. 2022
• NRSF-GNAO1 Pathway Contributes to the Regulation of Cardiac Ca2+ Homeostasis

  INAZUMI HIDEAKI, Graduate School of Medicine, Chief Examiner

  23 Mar. 2022
• Prediction of trabecular meshwork-targeted micro-invasive　glaucoma surgery outcomes using anterior segment OCT angiography

  OKAMOTO YOKO, Graduate School of Medicine, Sub-chief Examiner

  23 Mar. 2022
• Endogenous agonist-bound S1PR3 structure reveals determinants of G protein-subtype bias

  MAEDA SHINTARO, Graduate School of Medicine, Chief Examiner

  23 Mar. 2022
• Tranilast Inhibits TGF-β1-induced Epithelial-mesenchymal Transition and　Invasion/Metastasis via the Suppression of Smad4 in Human Lung Cancer Cell Lines

  TAKAHASHI KOJI, Graduate School of Medicine, Sub-chief Examiner

  23 Mar. 2022
• Evaluation of Weighted Diffusion Subtraction for Detection of Clinically Significant Prostate Cancer

  SATO TOSHIYUKI, Graduate School of Medicine, Sub-chief Examiner

  24 Nov. 2021
• Impairment of proteasome function in podocytes leads to chronic kidney disease

  MAKINO SHINICHI, Graduate School of Medicine, Sub-chief Examiner

  24 Sep. 2021
• Propranolol Attenuates Late Sodium Current in a Long QT Syndrome Type 3-Human Induced Pluripotent Stem Cell Model

  HIROSE SAYAKO, Graduate School of Medicine, Chief Examiner

  26 Jul. 2021
• Visualization of stem cell activity in pancreatic cancer expansion by direct lineage tracing with live imaging

  MARUNO TAKAHISA, Graduate School of Medicine, Sub-chief Examiner

  26 Jul. 2021

Part-time lecturer

• From 01 Apr. 2018, To 31 Mar. 2019

  東京大学, 大学院医学系研究科

Administration

School management (title, position)

• From 01 Apr. 2024, To 31 Mar. 2025

  国際高等教育院 企画評価専門委員会 数学部会
• From 01 Apr. 2024, To 31 Mar. 2026

  特定認定再生医療等委員会 委員
• From 01 Apr. 2024, To 31 Mar. 2026

  臨床研究審査委員会 委員
• From 01 Apr. 2023, To 31 Mar. 2024

  国際高等教育院 企画評価専門委員会 数学部会 委員
• From 01 Apr. 2022, To 31 Mar. 2024

  特定認定再生医療等委員会 委員
• From 01 Apr. 2022, To 31 Mar. 2024

  臨床研究審査委員会 委員
• From 01 Apr. 2022, To 31 Mar. 2024

  教職教育委員会 委員
• From 01 Apr. 2022, To 31 Mar. 2023

  国際高等教育院 企画評価専門委員会 数学部会 委員
• From 01 Apr. 2015, To 31 Mar. 2017

  学生生活委員会 委員
• From 01 Apr. 2015, To 31 Mar. 2017

  学生生活委員会第二小委員会 委員
• From 01 Apr. 2019, To 31 Mar. 2022

  大学評価委員会点検・評価実行委員会 委員
• From 01 Apr. 2019, To 31 Mar. 2020

  国際高等教育院 企画評価専門委員会 数学部会 委員
• From 01 Jun. 2018, To 31 Mar. 2020

  臨床研究審査委員会 委員
• From 01 Apr. 2019, To 31 Mar. 2021

  特定認定再生医療等委員会 委員
• From 01 Apr. 2019, To 31 Mar. 2021

  数学部会委員
• From 01 Apr. 2020, To 31 Mar. 2021

  国際高等教育院 企画評価専門委員会 数学部会 委員
• From 01 Apr. 2020, To 31 Mar. 2022

  臨床研究審査委員会 委員
• From 01 Apr. 2020, To 31 Mar. 2022

  特定認定再生医療等委員会 委員
• From 01 Apr. 2021, To 31 Mar. 2022

  国際高等教育院 企画評価専門委員会 数学部会 委員

Faculty management (title, position)

• From 01 Apr. 2024, To 31 Mar. 2025

  リサーチ・フェロー選考委員会
• From 01 Apr. 2024, To 31 Mar. 2025

  高次生命科学専攻長
• From 01 Dec. 2023, To 30 Nov. 2025

  医学研究科等防犯カメラ設置運用委員会委員
• From 01 Apr. 2023, To 31 Mar. 2024

  ダイバーシティ推進委員会
• From 01 Apr. 2023, To 31 Mar. 2025

  同窓会　役員
• From 01 Apr. 2023, To 31 Mar. 2025

  改善委員会
• From 01 Apr. 2023, To 31 Mar. 2024

  高次生命科学副専攻長
• From 01 Apr. 2015, To 31 Mar. 2016

  高次生命科学副専攻長
• From 01 Apr. 2015, To 31 Mar. 2017

  改善委員会委員
• From 01 Apr. 2015, To 31 Mar. 2017

  制度・財政委員会委員
• From 01 Apr. 2015, To 31 Mar. 2017

  生命科学研究科同窓会　役員
• From 01 Jun. 2015, To 31 Mar. 2017

  男女共同参画推進アクションプラン推進委員会
• From 01 Apr. 2015, To 31 Mar. 2019

  医学図書館運営委員会
• From 01 Feb. 2016, To 31 Mar. 2020

  医学研究科運営委員会
• From 01 Apr. 2016, To 31 Mar. 2017

  高次生命科学専攻長
• From 01 Dec. 2017, To 30 Nov. 2023

  医学研究科等防犯カメラ設置運用委員会委員
• From 01 Apr. 2018, To 31 Mar. 2020

  医学研究科学務委員会委員
• From 01 Nov. 2019, To 31 Oct. 2020

  医学研究科・期限付特定教員採用審査委員会
• From 01 Apr. 2019, To 31 Mar. 2021

  京都大学臨床研究審査委員会
• From 01 Apr. 2019, To 31 Mar. 2021

  京都大学大学院医学研究科・医学部および医学部附属病院医の倫理委員会
• From 01 Apr. 2019, To 31 Mar. 2021

  京都大学特定認定再生医療等委員会
• From 01 Nov. 2020, To 31 Oct. 2022

  期限付特定教員採用審査委員会委員