Basic Information

Affiliated programs (koza)

• Graduate School of Pharmaceutical Sciences, 薬科学専攻 生体機能化学講座, 助教

Faculty

• 薬学部

Professional Memberships

• From Jul. 2019, To Present

  The Membrane Society of Japan
• From Jun. 2017, To Present

  Division of Biofunctional Chemistry, The chemical Society of Japan
• From Aug. 2018, To Mar. 2021

  THE BIOPHYSICAL SOCIETY OF JAPAN
• From Apr. 2016, To Present

  The Japanese Peptide Society
• From Oct. 2011, To Present

  THE PHARMACEUTICAL SOCIETY OF JAPAN

Academic Degree

• 修士（薬学）(京都大学)
• 博士（薬学）(京都大学)

Academic Resume (Graduate Schools)

• 京都大学, 大学院薬学研究科修士課程創薬科学専攻, 修了
• 京都大学, 大学院薬学研究科博士後期課程創薬科学専攻, 修了

Research History

• From Apr. 2021, To Present

  Kyoto University, Graduate School of Pharmaceutical Sciences, Assistant Professor
• From Apr. 2016, To Mar. 2021

  Kyoto University, Institute for Chemical Research, Assistant Professor
• From Oct. 2015, To Mar. 2016

  Kyoto University, Institute for Integrated Cell-Material Sciences (iCeMS), Postdoctoral fellow
• From Jul. 2015, To Sep. 2015

  Kyoto University, Graduate School of Pharmaceutical Sciences, Postdoctoral fellow
• From Apr. 2014, To Jun. 2015

  Kyoto University, Institute for Virus Research, Limited-term Assistant Professor

Profile

• Profile

  現在の研究課題
  1. 曲率認識ペプチドを担体とした細胞外小胞の新規回収法の開発
  2. 細胞外小胞に由来するアジュバント活性物質の探索
  3. 新規可溶化剤を用いた膜タンパク質の構造解析研究

ID,URL

• ORCID ID

  0000-0003-1927-2922

• J-Global ID

  201401004373240099

• Researcher number

  70732874

• External link

  https://www.pharm.kyoto-u.ac.jp/yakkai/

researchmap URL

• https://researchmap.jp/7000009602

Research

Research Interests

• Extracellular vesicles
• membrane curvature
• drug delivery
• membrane proteins
• fluorescence imaging
• lipid packing
• peptide

Research Areas

• Life sciences, Pharmaceuticals - analytical and physicochemistry, 生物物理化学
• Life sciences, Pharmaceuticals - chemistry and drug development, Chemical Biology

Papers

• Optimizing Charge Switching in Membrane Lytic Peptides for Endosomal Release of Biomacromolecules

  Kentarou Sakamoto; Misao Akishiba; Takahiro Iwata; Kazuya Murata; Seiya Mizuno; Kenichi Kawano; Miki Imanishi; Fumihiro Sugiyama; Shiroh Futaki

  Angewandte Chemie, Aug. 2020

  
• Helicene–Fluorescein Hybrids: A Reversible Base-triggered (Chir)optical Switch with Sign Inversion of Circularly Polarized Luminescence

  Sorachi Miwa; Daichi Mizutani; Kenichi Kawano; Katsumi Matsuzaki; Yuuya Nagata; Kazunori Tsubaki; Kiyosei Takasu; Hiroshi Takikawa

  Chemistry - A European Journal, 30 Jan. 2025, Peer-reviewed

  
• Stable and minimum-size solubilization of membrane proteins with cocktails of phospholipid analogs

  Mai Takagi; Ayana Nagatani; Kenichi Kawano; Ayami Hata; Azusa Yokoyama; Koichi Hayashida; Haruka Hoshi; Mao Sakurai; Tsuyoshi Oyama; Yusuke Kuroda; Yousuke Yamaoka; Takahiro Fujiwara; Yohei Miyanoiri; Masaru Hoshino; Yoshiaki Yano; Kiyosei Takasu; Katsumi Matsuzaki

  ACS Applied Materials & Interfaces, 20 Nov. 2024, Peer-reviewed

  
• Development of Simple Methods for Detecting and Isolating Extracellular Vesicles using Curvature-sensing Peptides

  Kenichi Kawano

  Membrane, 04 Dec. 2024, Peer-reviewed, Invited, Lead author, Last author, Corresponding author

  
• Amyloid fibril formation on neuronal cells in the coexistence of Aβ40 and Aβ42

  Mayu Kawaguchi; Kenichi Kawano; Aoi Taniguchi; Atsushi Tanaka; Katsumi Matsuzaki

  ChemBioChem, 25 Sep. 2024, Peer-reviewed

  
• Rapid screening and identification of genes involved in bacterial extracellular membrane vesicle production using a curvature-sensing peptide

  Hiromu Inoue; Kenichi Kawano; Jun Kawamoto; Takuya Ogawa; Tatsuo Kurihara

  Journal of Bacteriology (in press), 04 Apr. 2025, Peer-reviewed

  
• Extracellular-vesicle catch-and-release isolation system using a net-charge invertible curvature-sensing peptide

  Kenichi Kawano; Yuki Kuzuma; Koichi Yoshio; Kenta Hosokawa; Yuuto Oosugi; Takahiro Fujiwara; Fumiaki Yokoyama; Katsumi Matsuzaki

  Analytical Chemistry, 05 Mar. 2024, Peer-reviewed, Lead author, Corresponding author

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• Elucidation of the Curved Membrane–binding Mechanism of Curvature–sensing Peptides that Enable Highly Sensitive Detection of Extracellular Vesicles

  Kenichi Kawano

  Membrane, 02 Aug. 2023, Peer-reviewed, Invited, Lead author, Last author, Corresponding author

  
• Structural factors governing binding of curvature-sensing peptides to bacterial extracellular vesicles covered with hydrophilic polysaccharide chains

  Kenichi Kawano; Kouhei Kamasaka; Fumiaki Yokoyama; Jun Kawamoto; Takuya Ogawa; Tatsuo Kurihara; Katsumi Matsuzaki

  Biophysical Chemistry, 18 May 2023, Peer-reviewed, Invited, Lead author, Corresponding author

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• Structural Dissection of Epsin-1 N-Terminal Helical Peptide: The Role of Hydrophobic Residues in Modulating Membrane Curvature

  Motoki Nishimura; Yoshimasa Kawaguchi; Kakeru Kuroki; Yuna Nakagawa; Toshihiro Masuda; Takayuki Sakai; Kenichi Kawano; Hisaaki Hirose; Miki Imanishi; Tomoka Takatani-Nakase; Sergii Afonin; Anne S. Ulrich; Shiroh Futaki

  Chemistry - A European Journal, 06 Mar. 2023, Peer-reviewed

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• CELL PENETRATION OF DIPICOLYLAMINE-MODIFIED R8 FACILITATED BY METAL COMPLEX FORMATION

  S. Ise; Y. Kawaguchi; T. Takeuchi; K. Kawano; S. Futaki

  JOURNAL OF PEPTIDE SCIENCE, Sep. 2016

  
• The Amantadine-Resistant S31N Mutant of Influenza a Virus M2 Protein Stably Forms a Dimer on the Living Cells

  Kenichi Kawano; Yoshiaki Yano; Katsumi Matsuzaki

  BIOPHYSICAL JOURNAL, Jan. 2014

  
• Dimer is a Minimal Functional Unit of Influenza a Virus M2 Channel on Living Cells

  Kenichi Kawano; Yoshiaki Yano; Katsumi Matsuzaki

  BIOPHYSICAL JOURNAL, Jan. 2014

  
• Influenza A virus M2 protein forms a dimeric channel in biomembranes

  K. Kawano; Y. Yano; K. Matsuzaki

  EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Jul. 2013

  
• Detection of Oligomerization of Membrane Proteins using Coiled-Coil Tag-Probe Labeling Method and In-Cell Fluorescence Spectroscopy

  Kenichi Kawano; Kaoru Omae; Sayaka Matsuzaki; Yoshiaki Yano; Katsumi Matsuzaki

  BIOPHYSICAL JOURNAL, Jan. 2013

  
• Oligomeric States of Full Length Influenza a Virus M2 Proteins on Biological Membranes

  Kenichi Kawano; Sayaka Matsuzaki; Kaoru Oomae; Yoshiaki Yano; Katsumi Matsuzaki

  BIOPHYSICAL JOURNAL, Jan. 2013

  
• Development of antimicrobial peptide-antibiotic conjugates to improve the outer membrane permeability of antibiotics against Gram-negative bacteria

  Ruka Yamauchi; Kenichi Kawano; Yousuke Yamaoka; Aoi Taniguchi; Yoshiaki Yano; Kiyosei Takasu; Katsumi Matsuzaki

  ACS Infectious Diseases, 18 Oct. 2022, Peer-reviewed, Lead author

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• Hemopexin as a Potential Binding Partner of Arginine-Rich Cell-Penetrating Peptides in Serum

  Kayo Nomura; Kenichi Kawano; Yoshimasa Kawaguchi; Yuki Kawamura; Junya Michibata; Keiko Kuwata; Koji Sugiyama; Kenji Kusumoto; Shiroh Futaki

  ACS Pharmacology & Translational Science, 20 Jul. 2022, Peer-reviewed, Lead author

  
• Split luciferase-based estimation of cytosolic cargo concentration delivered intracellularly via attenuated cationic amphiphilic lytic peptides

  Syusuke Okano; Yoshimasa Kawaguchi; Kenichi Kawano; Hisaaki Hirose; Miki Imanishi; Shiroh Futaki

  Bioorganic & Medicinal Chemistry Letters, 04 Jul. 2022, Peer-reviewed

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• Synthesis and Properties of V-Shaped Xanthene Dyes with Tunable and Predictable Absorption and Emission Wavelengths

  Akari Yamagami; Kotaro Kiyotaki; Sae Wakabayashi; Naoki Egami; Kenichi Kawano; Shiroh Futaki; Ayumi Imayoshi; Kazunori Tsubaki

  The Journal of Organic Chemistry, 12 Jan. 2022, Peer-reviewed

  
• Design of the N-terminus Substituted Curvature-sensing Peptides that Exhibit Highly Sensitive Detection Ability of Bacterial Extracellular Vesicles

  Kenichi Kawano; Fumiaki Yokoyama; Kouhei Kamasaka; Jun Kawamoto; Takuya Ogawa; Tatsuo Kurihara; Shiroh Futaki

  Chemical and Pharmaceutical Bulletin, 01 Nov. 2021, Peer-reviewed, Lead author, Corresponding author

  
• 細胞外膜小胞の高感度検出を基軸とした曲率認識ペプチドの創製研究

  Kenichi Kawano

  Peptide Newsletter Japan, 30 Jul. 2021, Peer-reviewed, Invited, Lead author, Last author, Corresponding author

  
• Influence of the Dabcyl group on the cellular uptake of cationic peptides: short oligoarginines as efficient cell-penetrating peptides

  Ildikó Szabó; Françoise Illien; Levente E. Dókus; Mo’ath Yousef; Zsuzsa Baranyai; Szilvia Bősze; Shoko Ise; Kenichi Kawano; Sandrine Sagan; Shiroh Futaki; Ferenc Hudecz; Zoltán Bánóczi

  Amino Acids, 25 May 2021, Peer-reviewed

  
• Membrane anchoring of a curvature-inducing peptide, EpN18, promotes membrane translocation of octaarginine

  Kakeru Kuroki; Takayuki Sakai; Toshihiro Masuda; Kenichi Kawano; Shiroh Futaki

  Bioorganic & Medicinal Chemistry Letters, 10 May 2021, Peer-reviewed

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• Metal complex lipids for fluid–fluid phase separation in co-assembled phospholipid membranes

  Ryo Ohtani; Yuka Anegawa; Hikaru Watanabe; Yutaro Tajima; Masanao Kinoshita; Nobuaki Matsumori; Kenichi Kawano; Saeko Yanaka; Koichi Kato; Masaaki Nakamura; Masaaki Ohba; Shinya Hayami

  Angewandte Chemie International Edition, 15 Mar. 2021, Peer-reviewed

  
• Development of a Microwave Resonator and Effects of Electric and Magnetic Fields on Cultured Cancer Cells

  Mamiko Asano; Jun-ichi Sugiyama; Kenichi Kawano; Shiroh Futaki

  Thermal Medicine, Mar. 2021, Peer-reviewed

  
• Development of a Simple and Rapid Method for In Situ Vesicle Detection in Cultured Media

  Kenichi Kawano; Fumiaki Yokoyama; Jun Kawamoto; Takuya Ogawa; Tatsuo Kurihara; Shiroh Futaki

  Journal of Molecular Biology, 12 Sep. 2020, Peer-reviewed, Lead author, Corresponding author

  
• Pseudo‐membrane jackets: Two‐dimensional coordination polymers achieving visible phase separation in cell membrane

  Ryo Ohtani; Kenichi Kawano; Masanao Kinoshita; Saeko Yanaka; Hikaru Watanabe; Kenji Hirai; Shiroh Futaki; Nobuaki Matsumori; Hiroshi Uji-i; Masaaki Ohba; Koichi Kato; Shinya Hayami

  Angewandte Chemie International Edition, 30 Jun. 2020, Peer-reviewed, Lead author

  
• Optimizing charge switching in membrane lytic peptides for endosomal release of biomacromolecules

  Kentarou Sakamoto; Misao Akishiba; Takahiro Iwata; Kazuya Murata; Seiya Mizuno; Kenichi Kawano; Miki Imanishi; Fumihiro Sugiyama; Shiroh Futaki

  Angewandte Chemie International Edition, 18 Jun. 2020, Peer-reviewed

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• Rational Design Principles of Attenuated Cationic Lytic Peptides for Intracellular Delivery of Biomacromolecules

  Naoki Tamemoto; Misao Akishiba; Kentarou Sakamoto; Kenichi Kawano; Hiroshi Noguchi; Shiroh Futaki

  Molecular Pharmaceutics, Apr. 2020, Peer-reviewed

  
• Enhancing the Activity of Membrane Remodeling Epsin-Peptide by Trimerization

  Wei-Yuan Hsu, Toshihiro Masuda, Sergii Afonin, Takayuki Sakai, Jan Vincent V Arafiles, Kenichi Kawano, Hisaaki Hirose, Miki Imanishi, Anne S Ulrich, Shiroh Futaki

  Bioorganic & Medicinal Chemistry Letters, Apr. 2020, Peer-reviewed

  
• Loosening of Lipid Packing by Cell Surface Recruitment of Amphiphilic Peptides by Coiled-coil Tethering

  Takayuki Sakai; Kenichi Kawano; Masatomo Iino; Toshihide Takeuchi; Miki Imanishi; Shiroh Futaki

  ChemBioChem, Aug. 2019, Peer-reviewed

  
• Development of a membrane curvature-sensing peptide based on a structure–activity correlation study.

  Kenichi Kawano; Masaya Ogushi; Toshihiro Masuda; Shiroh Futaki

  Chemical & Pharmaceutical Bulletin, Jul. 2019, Peer-reviewed, Lead author

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• Dipicolylamine/Metal Complexes that Promote Direct Cell-Membrane Penetration of Octaarginine.

  Kawaguchi Y; Ise S; Azuma Y; Takeuchi T; Kawano K; Le TK; Ohkanda J; Futaki S

  Bioconjugate chemistry, Feb. 2019, Peer-reviewed

  
• Development of xanthene dyes containing arylacetylenes: The role of acetylene linker and substituents on the aryl group

  Ryo Hirata; Ayame Torii; Kenichi Kawano; Shiroh Futaki; Ayumi Imayoshi; Kazunori Tsubaki

  Tetrahedron, 05 Jul. 2018, Peer-reviewed

  
• Importance of Net Hydrophobicity in the Cellular Uptake of All-Hydrocarbon Stapled Peptides

  Koki Sakagami; Toshihiro Masuda; Kenichi Kawano; Shiroh Futaki

  Molecular Pharmaceutics, 05 Mar. 2018, Peer-reviewed

  
• Syntheses and properties of second-generation V-shaped xanthene dyes with piperidino groups

  Akari Yamagami; Kenichi Kawano; Shiroh Futaki; Kouji Kuramochi; Kazunori Tsubaki

  Tetrahedron, Dec. 2017, Peer-reviewed

  
• ペプチド科学を基盤技術に用いた膜タンパク質と生体膜の研究

  河野 健一

  Peptide Newsletter Japan, Nov. 2017, Peer-reviewed, Lead author, Last author, Corresponding author

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• Stoichiometric analysis of oligomeric states of three class-A GPCRs, chemokine-CXCR4, dopamine-D2, and prostaglandin-EP1 receptors, on living cells

  Kenichi Kawano; Tetsuya Yagi; Nozomu Fukada; Yoshiaki Yano; Katsumi Matsuzaki

  Journal of Peptide Science, Jul. 2017, Peer-reviewed, Lead author

  
• Loosening of Lipid Packing Promotes Oligoarginine Entry into Cells

  Tomo Murayama; Toshihiro Masuda; Sergii Afonin; Kenichi Kawano; Tomoka Takatani-Nakase; Hiroki Ida; Yasufumi Takahashi; Takeshi Fukuma; Anne S. Ulrich; Shiroh Futaki

  Angewandte Chemie International Edition, Jun. 2017, Peer-reviewed

  
• A pH-dependent charge reversal peptide for cancer targeting

  Naoko Wakabayashi; Yoshiaki Yano; Kenichi Kawano; Katsumi Matsuzaki

  European Biophysical Journal, Mar. 2017, Peer-reviewed

  
• 

  The 3G Condiments, Garlic, Ginger, and Green Onion, Exhibit Synergistic Antibacterial effects

  望月 達人; 河野 健一; 矢野 義明; 松崎 勝巳

  ELCAS Journal, Mar. 2016

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• Oligomerization-function relationship of EGFR on living cells detected by the coiled-coil labeling and FRET microscopy

  Hirotaka Yamashita; Yoshiaki Yano; Kenichi Kawano; Katsumi Matsuzaki

  Biochimica et Biophysica Acta - Biomembranes, 2015, Peer-reviewed

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• 

  A Dimer Is the Minimal Proton-Conducting Unit of the Influenza A Virus M2 Channel

  Kenichi Kawano; Yoshiaki Yano; Katsumi Matsuzaki

  Journal of Molecular Biology, Jul. 2014, Peer-reviewed, Lead author

  
• A visualization tool for oligomerization and internalization of membrane proteins in living cells: Coiled-coil labeling method

  Yoshiaki Yano; Kenichi Kawano; Kaoru Omae; Yuki Takeda; Sayaka Matsuzaki; Katsumi Matsuzaki

  Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 2014, Peer-reviewed

  
• Stoichiometric analysis of oligomerization of membrane proteins using coiled-coil labeling and in-cell spectroscopy

  Kenichi Kawano

  Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 2014, Peer-reviewed, Lead author

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• 

  Stoichiometric analysis of oligomerization of membrane proteins on living cells using coiled-coil labeling and spectral imaging

  Kenichi Kawano; Yoshiaki Yano; Kaoru Omae; Sayaka Matsuzaki; Katsumi Matsuzaki

  Analytical Chemistry, 19 Mar. 2013, Peer-reviewed, Lead author

  
• 

  Coiled-Coil Tag–Probe Labeling Methods for Live-Cell Imaging of Membrane Receptors

  Yoshiaki Yano; Kenichi Kawano; Kaoru Omae; Katsumi Matsuzaki

  Methods Enzymology, 2012, Peer-reviewed

  

Misc.

• 糖鎖に覆われた細胞外小胞膜に結合する曲率認識ペプチドの構造的柔軟性の重要さ

  河野健一; 釜阪紘平; 横山文秋; 横山文秋; 川本純; 小川拓哉; 栗原達夫; 松崎勝巳

  生体膜と薬物の相互作用シンポジウム講演要旨集, 2023

  
• 蛍光共鳴エネルギー移動を用いた曲率認識ペプチドによる細胞外小胞の高感度検出法の開発

  河野健一; 横山文秋; 横山文秋; 小川拓哉; 川本純; 栗原達夫; 松崎勝巳

  次世代を担う若手のためのフィジカル・ファーマフォーラム講演要旨集, 2022

  
• ペプチド・タンパク質医薬のサイトゾル送達量の定量的評価系構築

  岡野 秀亮; 川口 祥正; 河野 健一; 二木 史朗

  日本薬学会年会要旨集, Mar. 2022

  
• オクタアルギニンを介した細胞内タンパク質送達法の細胞質輸送効率を評価するためのMALDI-TOF型質量分析計を用いた分析法(A MALDI-TOF mass spectroscopy-based approach to evaluate the cytosolic translocation efficiency of octaarginine-mediated intracellular protein delivery)

  Arafiles Jan Vincent; 川口 祥正; Cherine Bechara; Erancoise Illien; 河野 健一; Fabienne Burlina; Sandrine Sagan; 二木 史朗

  日本薬学会年会要旨集, Mar. 2017

  
• オクタアルギニンと血清タンパク質の相互作用

  野村 佳世; 河野 健一; 川口 祥正; 桑田 啓子; 二木 史朗

  日本薬学会年会要旨集, Mar. 2017

  
• 1P297 Development of methods for labeling and oligomerization analysis of membrane proteins in live cells(27. Bioimaging,Poster)

  Yano Yoshiaki; Kawano Kenichi; Omae Kaoru; Mtsuzaki Sayaka; Matsuzaki Katsumi

  Seibutsu Butsuri, 2013

  

Presentations

• Stoichiometric analysis of oligomeric states of membrane proteins and sensing analysis of membrane curvature of extracellular vesicles

  Kenichi Kawano

  The 28th Korean Peptide and Protein Society (KPPS) Annual Symposium, 24 Jun. 2025, Invited

  
• Applied research on extracellular vesicles based on membrane curvature-sensing technology.

  K. Kawano

  日本膜学会第47年会（奨励賞受賞講演）, 05 Jun. 2025, Invited

  
• Exploration of immunostimulatory molecules in extracellular vesicles secreted from a horse mackerel intestinal bacterium.

  K. Yoshio; J. Kawamoto; T. Kurihara; K. Kawano

  日本膜学会第47年会, Jun. 2025

  
• Multiple applications of extracellular vesicles using curvature-sensing peptides

  Kenichi Kawano

  Pacifichem 2025 (Symposium: Advances in Peptide Therapeutics and Peptidomimetics: Navigating the Future of Precision Medicine), Dec. 2025, Invited

  
• THE AMANTADINE-RESISTANT S31N MUTANT OF INFLUENZA A VIRUS M2 PROTEIN STABLY FORMS A DIMER ON THE LIVING CELLS

  Kenichi Kawano; Yoshiaki Yano; Katsumi Matsuzaki

  58th Annual Meeting Biophysical Society, 16 Feb. 2014

  
• OLIGOMERIC STATES OF FULL LENGTH INFLUENZA A VIRUS M2 PROTEINS ON BIOLOGICAL MEMBRANES

  Ken-ichi Kawano; Sayaka Matsuzaki; Kaoru Oomae; Yoshiaki Yano; Katsumi Matsuzaki

  57th Annual Meeting Biophysical Society, 04 Feb. 2013

  
• 可溶化GPCRのリガンド結合能評価系による可溶化剤各種の特性探索

  星遥華; 河野健一; 藤原敬宏; 山岡庸介; 黒田悠介; 高須清誠; 松崎勝巳

  第2回関西生物物理学研究会, 23 Mar. 2025

  
• 低pH応答抗がん性ペプチドをベースとした薬物conjugateとカチオン性リポソームDDS

  谷口葵; 河野健一; 松崎勝巳

  第2回関西生物物理学研究会, 22 Mar. 2025

  
• Optimization of extracellular-vesicle catch-and-release isolation system using a net-charge invertible curvature-sensing peptide

  大杉悠斗; 河野健一; 松崎勝巳

  第2回関西生物物理学研究会, 22 Mar. 2025

  
• A型インフルエンザウイルスM2タンパク質の構造解析

  河野健一; 櫻井真央; 黒田悠介; 高須清誠; 星野大; 松崎勝巳

  第2回関西生物物理学研究会, 22 Mar. 2025

  
• SELECTIVE ANTICANCER ACTIVITIES OF LOW PH-RESPONSIVE PEPTIDES AND ACTIVITY ENHANCEMENT BY ANTICANCER DRUG CONJUGATION AND CATIONIC LIPOSOMES

  Aoi Taniguchi; Kenichi Kawano; Katsumi Matsuzaki

  The 61st Japanese Peptide Symposium, 29 Oct. 2024

  
• ALANINE SCANNING REVEALED THE KEY CHARGED AMINO ACID RESIDUES OF Aβ40 AFFECTING ITS FIBRIL FORMATION AND CYTOTOXICITY

  Atsushi Tanaka; Kenichi Kawano; Katsumi Matsuzaki

  The 61st Japanese Peptide Symposium, 29 Oct. 2024

  
• OPTIMIZATION OF EXTRACELLULAR-VESICLE CATCH-AND-RELEASE ISOLATION SYSTEM USING A NET-CHARGE INVERTIBLE CURVATURE-SENSING PEPTIDE

  Yuuto Oosugi; Kenichi Kawano; Katsumi Matsuzaki

  The 61st Japanese Peptide Symposium, 29 Oct. 2024

  
• Development of extracellular-vesicle catch-and-release isolation system using a net-charge invertible curvature-sensing peptide

  河野健一; 葛馬佑樹; 吉尾航一; 細川健太; 大杉悠斗; 藤原敬宏; 横山文秋; 松崎勝巳

  The 18th Symposium on Biorelevant Chemistry, 12 Sep. 2024

  
• pH依存的曲率認識ペプチドを用いた細胞外小胞単離法の開発

  河野 健一; 葛馬 佑樹; 吉尾 航一; 細川 健太; 大杉 悠斗; 藤原 敬宏; 横山 文秋; 松﨑 勝巳

  膜シンポジウム2024, 28 Nov. 2024

  
• BIOMEMBRANE RESEARCH UTILIZING FUNCTIONAL PEPTIDES

  Kenichi Kawano

  The 61st Japanese Peptide Symposium, 31 Oct. 2024, Invited

  
• 曲率認識ぺプチドを用いたエクソソームへの機能性ペプチド修飾技術の開発

  中坊美穂; 河野健一; 藤原大佑; 道上雅孝; 服部能英; 切畑光統; 松崎勝巳; 藤井郁夫; 中瀬生彦

  日本膜学会 第46年会, 11 Jun. 2024

  
• 高価な装置を必要としない細胞外小胞のキャッチ&リリース単離法の開発

  河野健一

  第14回CSJ化学フェスタ2024, 23 Oct. 2024, Invited

  
• Extracellular-vesicle catch-and-release isolation system using a net-charge invertible curvature-sensing peptide

  Kenichi Kawano; Yuki Kuzuma; Koichi Yoshio; Kenta Hosokawa; Yuuto Oosugi; Takahiro Fujiwara; Fumiaki Yokoyama; Katsumi Matsuzaki

  The 37th EPS and 14th IPS Symposia, 26 Aug. 2024

  
• 曲率認識ペプチドを用いた微生物の細胞外小胞検出と結合様式の物理化学的理解

  河野健一

  日本膜学会第46年会 生体膜シンポジウム, 12 Jun. 2024, Invited

  
• Improvement of membrane curvature-inducing ability of the EpN18 peptide by membrane anchoring

  Kakeru Kuroki; Takayuki Sakai; Kenichi Kawano; Shiroh Futaki

  The 56th Japanese Peptide Symposium, 10 Nov. 2020

  
• Rational design of cytosolic macromolecular delivery peptide with enhanced endosomolytic activity

  Kentarou Sakamoto; Misao Akishiba; Takahiro Iwata; Kenichi Kawano; Shiroh Futaki

  The 56th Japanese Peptide Symposium, 09 Nov. 2020

  
• Improving the activity of L17E by endowing pH-sensitive membrane destabilizing activity

  Kentarou Sakamoto; Misao Akishiba; Takahiro Iwata; Kenichi Kawano; Shiroh Futaki

  The 56th Japanese Peptide Symposium, 25 Oct. 2019

  
• Loosening of lipid packing by recruitment of amphiphilic peptides onto cell surface

  Takayuki Sakai; Kenichi Kawano; Miki Imanishi; Shiroh Futaki

  The 56th Japanese Peptide Symposium, 24 Oct. 2019

  
• Structural parameters contributing to the ability of amphipathic peptides to induce membrane curvature

  Motoki Nishimura; Toshihiro Masuda; Sergii Afonin; Kenichi Kawano; Anne S. Ulrich; Shiroh Futaki

  The 56th Japanese Peptide Symposium, 23 Oct. 2019

  
• Improving Endosomolytic Activity of L17E

  Kentarou Sakamoto; Misao Akishiba; Kenichi Kawano; Shiroh Futaki

  The 55th Japanese Peptide Symposium / The 10th IPS, 06 Dec. 2018

  
• Identified Macropinocytosis-inducing Peptide and Its Mode-of-Action

  Jan Vincent-V. Arafiles; Kenichi Kawano; Shiroh Futaki

  The 55th Japanese Peptide Symposium / The 10th IPS, 05 Dec. 2018

  
• Detecting Highly Curved Membranes by Amphipathic Peptides

  Masaya Ogushi; Toshihiro Masuda; Kenichi Kawano; Shiroh Futaki

  The 55th Japanese Peptide Symposium / The 10th IPS, 04 Dec. 2018

  
• Promoting Accumulation of Curvature-inducing Peptides on Cell Membranes

  Takayuki Sakai; Kenichi Kawano; Shiroh Futaki

  The 55th Japanese Peptide Symposium / The 10th IPS, 04 Dec. 2018

  
• Structural Determinants of Membrane Curvature Inducing Peptide

  Motoki Nishimura; Toshihiro Masuda; Kenichi Kawano; Shiroh Futaki

  The 55th Japanese Peptide Symposium / The 10th IPS, 04 Dec. 2018

  
• Novel macropinocytosis-inducing cell penetrating peptides

  Jan Vincent-V. Arafiles; Kenichi Kawano; Shiroh Futaki

  The 54th Japanese Peptide Symposium, 21 Nov. 2017

  
• Histidines in L17E endosome-destabilizing peptide

  Kentarou Sakamoto; Misao Akishiba; Kenichi Kawano; Shiroh Futaki

  The 54th Japanese Peptide Symposium, 20 Nov. 2017

  
• Dimer is a minimal functional unit for influenza a virus m2 channel on biomembrane

  Kenichi Kawano; Yoshiaki Yano; Katsumi Matsuzaki

  The 50th Japanese Peptide Symposium / The 4th APIPS, 06 Nov. 2013

  
• Surface Functionalization For Marker Independent EV Capture And Profiling

  Hugues Martin; Andreas Wallucks; Andy Ng; Molly Shen; Kenichi Kawano; David Juncker

  ISEV2024 Annual Meeting, May 2024

  
• Shewanella vesiculosa HM13 の細胞外膜小胞生産関連遺伝子の高効率スクリーニングと生理機能解析

  井上宙夢; 河野健一; 川本 純; 小川 拓哉; 栗原達夫

  2024年度生物工学若手研究者の集い（若手会）夏のセミナー2024, Jul. 2024

  
• ANALYSIS OF THE HYPER-VESICULATING MECHANISM OF SHEWANELLA VESICULOSA HM13 USING A CURVATURE-SENSING PEPTIDE

  Hiromu Inoue; Kenichi Kawano; Jun Kawamoto; Takuya Ogawa; Tatsuo Kurihara

  14th International Congress on Extremophiles, Sep. 2024

  
• 曲率認識ペプチドを用いた乳酸菌の膜小胞産生条件の検討

  中村 めい; 前川 沙喜子; 河野 健一; 片倉 啓雄; 山崎 思乃

  日本農芸化学会2024年度大会, 26 Mar. 2024

  
• 膜曲率認識ペプチドを利用したエクソソーム膜修飾技術の開発とDDS応用

  中坊 美穂; 河野 健一; 藤原 大佑; 道上 雅孝; 服部 能英; 切畑 光統; 松崎 勝巳; 藤井 郁雄; 中瀬 生彦

  第1回 関西生物物理学研究会, 18 Mar. 2024

  
• 曲率認識ぺプチドによるエクソソーム膜へのアルギニンペプチド修飾と細胞内導入促進

  中坊 美穂; 河野 健一; 藤原 大佑; 道上 雅孝; 松崎 勝巳; 藤井 郁雄; 中瀬 生彦

  The 144th Annual Meeting of the Pharmaceutical Society of Japan, 30 Mar. 2024

  
• 曲率認識ペプチドを利用したエクソソーム膜修飾とDDSへの応用

  中坊 美穂; 河野 健一; 藤原 大佑; 道上 雅孝; 松崎 勝巳; 藤井 郁雄; 中瀬 生彦

  第38回関西地区ペプチドセミナー, 09 Dec. 2023

  
• 曲率認識ペプチドを利用したエクソソーム膜修飾と細胞内導入への応用

  中坊 美穂; 河野 健一; 藤原 大佑; 道上 雅孝; 松崎 勝巳; 藤井 郁雄; 中瀬 生彦

  BioMedical Forum2024, 05 Dec. 2023

  
• Selective anticancer activities of low pH-responsive anticancer peptides and a peptide−anticancer drug conjugate

  谷口 葵; 河野 健一; 松崎 勝巳

  The 144th Annual Meeting of the Pharmaceutical Society of Japan, 30 Mar. 2024

  
• Functional evaluation of extracellular vesicles isolated by a novel EV-isolation method using a curvature-sensing peptide

  Kenichi Kawano; Yuki Kuzuma; Koichi Yoshio; Kenta Hosokawa; Yuuto Oosugi; Takahiro Fujiwara; Fumiaki Yokoyama; Katsumi Matsuzaki

  The 144th Annual Meeting of the Pharmaceutical Society of Japan, 31 Mar. 2024

  
• Evaluation of Ligand Binding Ability of GPCRs Sollubilized with Novel Phospholipid Derivatives

  星 遥華; 河野 健一; 藤原 敬宏; 黒田 悠介; 山岡 庸介; 高須 清誠; 松崎 勝巳

  The 144th Annual Meeting of the Pharmaceutical Society of Japan, 30 Mar. 2024

  
• 電荷反転型曲率認識ペプチドを用いた細胞外小胞の簡便な回収法の開発

  河野健一; 葛馬佑樹; 藤原敬宏; 横山文秋; 松﨑勝巳

  「日本膜学会第45年会」・「膜シンポジウム2023」合同大会, 20 Nov. 2023

  
• A simple method to modify the surface of extracellular vesicle-mimicking liposomes using curvature-sensing peptides

  Kenta Hosokawa; Kenichi Kawano; Katsumi Matsuzaki

  The 60th Japanese Peptide Symposium, 09 Nov. 2023

  
• Extracellular-vesicle catch-and-release isolation system using a ph-dependent EV-binding curvature-sensing peptide

  Kenichi Kawano; Yuki Kuzuma; Takahiro Fujiwara; Fumiaki Yokoyama; Katsumi Matsuzaki

  The 60th Japanese Peptide Symposium, 09 Nov. 2023

  
• 糖鎖に覆われた細胞外小胞膜に結合する曲率認識ペプチドの構造的柔軟性の重要さ

  河野健一; 釜阪紘平; 横山文秋; 川本純; 小川拓哉; 栗原達夫; 松崎勝巳

  第44回 生体膜と薬物の相互作用シンポジウム, 20 Oct. 2023

  
• 神経細胞膜上におけるAβ40と42共存下での線維形成機構

  川口真由; 河野健一; 松崎勝巳

  第44回 生体膜と薬物の相互作用シンポジウム, 19 Oct. 2023

  
• 乳酸菌が産生する細胞外膜小胞の高生産条件の検討

  前田 朝海; 倉田 淳志; 河野 健一; 上垣 浩一

  第75回日本生物工学会大会（2023）, 04 Sep. 2023

  
• 曲率認識ペプチドの細胞外小胞結合を支配する構造的要因の解明

  河野健一; 釜阪紘平; 横山文秋; 川本純; 小川拓哉; 栗原達夫; 松崎; 勝巳

  第17回バイオ関連化学シンポジウム, 08 Sep. 2023

  
• 核酸含量から見た細菌細胞外膜小胞の多様性とそれらの生産に関わる遺伝子群

  井上 宙夢; 河野 健一; 川本 純; 小川 拓哉; 栗原 達夫

  第96回日本生化学会大会, 2023

  
• 乳酸菌細胞外膜小胞の高生産条件の検討

  前田 朝海; 倉田 淳志; 河野 健一; 上垣 浩一

  日本乳酸菌学会2023年度大会, 07 Jul. 2023

  
• Importance of the structural flexibility of curvature-sensing peptides that enable highly sensitive detection of extracellular vesicles

  Kenichi Kawano

  Biological Chemistry on Membranes - Understanding and Engineering -, 28 Aug. 2023, Invited

  
• Shewanella vesiculosa HM13の細胞外膜小胞生産関連 遺伝子の生理機能解析

  井上宙夢; 河野健一; 川本純; 小川拓哉; 栗原達夫

  日本農芸化学会関西支部 第525回講演会, 27 May 2023

  
• 曲率認識ペプチドを用いたプロバイオティクス由来メンブランベシクルの簡便な定量法の開発

  中村 めい; 前川 沙喜子; 河野 健一; 片倉 啓雄; 山﨑 思乃

  日本乳酸菌学会2023年度大会, 07 Jul. 2023

  
• Development of dimerized curvature-sensing peptides for detection of extracellular vesicles from bacteria with low EV-secretion levels

  Kenichi Kawano; Yuki Kuzuma; Yosuke Yamaoka; Kiyosei Takasu; Katsumi Matsuzaki

  The 143rd Annual Meeting of the Pharmaceutical Society of Japan, 28 Mar. 2023

  
• Design of a novel pH-responsive curvature-sensing peptide for isolation of extracellular vesicles

  Yuki Kuzuma; Kenichi Kawano; Katsumi Matsuzaki

  The 143rd Annual Meeting of the Pharmaceutical Society of Japan, 28 Mar. 2023

  
• Screening and functional prediction of genes related to membrane vesicle production of Shewanella vesiculosa HM13 using a curvature-sensing peptide

  Hiromu INOUE; Kenichi KAWANO; Jun KAWAMOTO; Takuya OGAWA; Tatsuo KURIHARA

  The 2023 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA), Mar. 2023

  
• Binding characteristics of a curvature-sensing peptide recognizing bacterial extracellular membrane vesicles

  Daiki Sakamoto; Hiromu Inoue; Kenichi Kawano; Tomoya Imai; Jun Kawamoto; Takuya Ogawa; Atsushi Kurata; Tatsuo Kurihara

  The 2023 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA), Mar. 2023

  
• 曲率認識ペプチドを用いた細菌細胞外膜小胞生産関連遺伝子のゲノム網羅的探索と機能推定

  井上宙夢; 河野健一; 川本 純; 小川 拓哉; 栗原達夫

  極限環境生物学会2022年度年会, 13 Nov. 2022

  
• 夾雑環境下における細胞外小胞の高感度検出を目指した曲率認識ペプチドの創製研究

  河野健一

  第123回生物科学フロンティアセミナー兼第43回ケミカルバイオロジー研究所セミナー, 18 Oct. 2022, Invited

  
• 両親媒性ペプチドの細胞膜集積化とその膜 相互作用の増強

  阪井貴之; 河野健一; 飯野真丈; 武内敏秀; 今西未来; 二木史朗

  第17回次世代を担う若手のためのフィジカル•ファーマフォーラム（PPF2019）, 03 Sep. 2019

  
• STRUCTURAL FACTORS GOVERNING VESICLE DETECTION OF CURVATURE-SENSING PEPTIDES

  Kenichi Kawano; Fumiaki Yokoyama; Kouhei Kamasaka; Jun Kawamoto; Takuya Ogawa; Tatsuo Kurihara; Katsumi Matsuzaki

  The 59th Japanese Peptide Symposium, 27 Oct. 2022

  
• DEVELOPMENT OF PH-RESPONSIVE ANTICANCER PEPTIDES

  Aoi Taniguchi; Kenichi Kawano; Katsumi Matsuzaki

  The 59th Japanese Peptide Symposium, 26 Oct. 2022

  
• 蛍光共鳴エネルギー移動を用いた曲率認識ペプチドによる細胞外小胞の高感度検出法の開発

  河野健一; 横山文秋; 小川拓哉; 川本純; 栗原達夫; 松﨑勝巳

  第19回次世代を担う若手のためのフィジカル・ファーマフォーラム(PPF2022), 13 Sep. 2022

  
• Development of curvature-sensing peptides for highly sensitive detection of extracellular vesicles

  KAWANO, Kenichi; KUZUMA, Yuki; YOKOYAMA, Fumiaki; KAMASAKA, Kouhei; OGAWA, Takuya; KAWAMOTO, Jun; KURIHARA, Tatsuo; MATSUZAKI, Katsumi

  The 16th Symposium on Biorelevant Chemistry, 11 Sep. 2022

  
• 曲率認識ペプチドを基軸とした細胞外小胞の研究

  河野健一

  第54回若手ペプチド夏の勉強会, 09 Aug. 2022, Invited

  
• Highly sensitive detection of extracellular vesicles with N-terminus-substituted curvature-sensing peptides

  Kenichi Kawano; Yuki Kuzuma; Fumiaki Yokoyama; Kouhei Kamasaka; Takuya Ogawa; Jun Kawamoto; Tatsuo Kurihara; Shiroh Futaki; Katsumi Matsuzaki

  The 142 Annual Meeting of The Pharmaceutical Society of Japan, 26 Mar. 2022

  
• Search for genes related to bacterial extracellular membrane vesicle production by high-throughput screening using curvature-sensing peptide

  井上宙夢; 河野健一; 川本純; 小川拓哉; 栗原達夫

  The 2022 Annual Meetings of The Japanese Society for Bioscience, Biotechnology and Agrochemistry (JSBBA), 18 Mar. 2022

  
• A novel detection tool of extracellular membrane vesicles of bacterial cells in cultured medium using a curvature-sensing peptide

  Kenichi Kawano; Fumiaki Yokoyama; Jun Kawamoto; Takuya Ogawa; Tatsuo Kurihara; Shiroh Futaki

  EMBO BMVs Workshop, 24 Nov. 2021

  
• 曲率認識ペプチドを用いたShewanella vesiculosa HM13の細胞外膜小胞生産関連遺伝子の探索

  井上宙夢; 河野健一; 川本 純; 小川 拓哉; 栗原 達夫

  極限環境生物学会2021年度（第22回）年会, 20 Nov. 2021

  
• 生細胞膜中で形成されるアミロイドβタンパク質線維の構造解析

  角友登; 河野健一; 矢野義明; 星野大; 松崎勝巳

  第42回生体膜と薬物の相互作用シンポジウム, 28 Oct. 2021

  
• DESIGN OF CURVATURE-SENSING PEPTIDES FOR HIGHLY SENSITIVE DETECTION OF BACTERIAL EXTRACELLULAR VESICLES

  Kenichi Kawano; Fumiaki Yokoyama; Kouhei Kamasaka; Jun Kawamoto; Takuya Ogawa; Tatsuo Kurihara; Shiroh Futaki

  The 58th Japanese Peptide Symposium, 21 Oct. 2021

  
• DEVELOPMENT OF ANTIMICROBIAL PEPTIDE–ANTIBIOTIC CONJUGATES FOR IMPROVED ANTIMICROBIAL ACTIVITY

  Ruka Yamauchi; Kenichi Kawano; Daigo Kajiwara; Yousuke Yamaoka; Yoshiaki Yano; Kiyosei Takasu; Katsumi Matsuzaki

  The 58th Japanese Peptide Symposium, 20 Oct. 2021

  
• 抗菌活性の向上を目指した抗菌性ペプチド-抗生物質conjugatesの開発

  山内瑠花; 河野健一; 梶原大悟; 山岡庸介; 矢野義明; 高須清誠; 松崎勝巳

  第53回若手ペプチド夏の勉強会, 10 Aug. 2021

  
• 細胞外小胞の高感度検出に向けた曲率認識ペプチドの設計指針

  河野健一; 横山文秋; 釜阪紘平; 小川拓哉; 川本純; 栗原達夫; 二木史朗

  日本膜学会第43年会, 05 Jun. 2021

  
• 曲率認識ペプチドを用いた細胞外膜小胞の簡便な検出法の開発

  河野健一

  日本薬学会第141年会, 29 Mar. 2021, Invited

  
• 曲率認識ペプチドを用いた細菌の菌体外膜小胞のハイスループット検出法の開発

  河野 健一; 小川 拓哉

  京都大学化学研究所 第120回化学研究所研究発表会, 11 Dec. 2020

  
• CURVATURE-SENSING PEPTIDES TO DISTINGUISH BACTERIAL EXTRACELLULAR MEMBRANE VESICLES FROM CELLS IN CULTURED MEDIUM

  K. Kawano; F. Yokoyama; J. Kawamoto; T. Ogawa; T. Kurihara; S. Futaki

  The 57th Japanese Peptide Symposium, 11 Nov. 2020

  
• 夾雑環境下における曲率認識ペプチドを用いた細胞外膜小胞の検出法の開発

  河野健一、横山文秋、小川拓哉、川本純、栗原達夫、二木史朗

  日本膜学会第42年会, 01 Jun. 2020

  
• 脂質パッキング過疎部位からのポリアルギニンペプチドの細胞内流入

  河野 健一 、阪井 貴之 、村山 知 、今西 未来 、二木 史朗

  第62回 日本脂質生化学会(JCBL2020), 14 May 2020, Invited

  
• Frontier research in membrane curvature on roles as molecular reaction sites

  Kenichi Kawano, Ikuhiko Nakase

  The 140th Annual Meeting of the Pharmaceutical Society of Japan, 27 Mar. 2020, Invited

  
• 膜曲率誘導による膜透過性ペプチドの細胞内送達促進

  河野健一

  IRCCS The 5th Symposium, 18 Nov. 2019, Invited

  
• 人工ドメイン形成による膜物性および細胞形態の変化

  河野 健一、高橋 康史、大谷 亮、木下 祥尚

  膜シンポジウム2019, 13 Nov. 2019

  
• DEVELOPMENT OF A MEMBRANE CURVATURE-SENSING PEPTIDE BASED ON A STRUCTURE–ACTIVITY CORRELATION STUDY

  Kenichi Kawano, Masaya Ogushi, Toshihiro Masuda, and Shiroh Futaki

  The 56th Japanese Peptide Symposium, 23 Oct. 2019

  
• Induction and reversible control of phase separation on living cell membranes

  Kenichi KAWANO; Yasufumi TAKAHASHI; Ryo OHTANI; Masanao KINOSHITA; Shiroh FUTAKI

  The 57th Annual Meeting of The Biophysical Society of Japan, 24 Sep. 2019

  
• 錯体脂質を用いた生細胞膜上における人工ドメイン構築

  河野 健一; 高橋 康史; 大谷 亮; 木下 祥尚

  第13回バイオ関連化学シンポジウム2019, 04 Sep. 2019

  
• 生細胞膜上における相分離の誘発と可逆的制御

  河野健一; 高橋康史; 大谷亮; 木下祥尚; 二木史朗

  第17回次世代を担う若手のためのフィジカル•ファーマフォーラム（PPF2019）, 03 Sep. 2019

  
• 膜曲率誘導によるオクタアルギニンの膜透過促進

  河野 健一; 村山知; 阪井貴之; 二木史朗

  第3回 統合物質創製化学研究推進機構(IRCCS) 若手の会, 12 Jul. 2019

  
• ペプチドを用いた膜曲率センシング

  河野 健一; 小串 正哉; 益田 俊博; 二木 史朗

  第14回ケミカルバイオロジー学会, 12 Jun. 2019

  
• ペプチドを用いた膜曲率センシング

  河野 健一; 小串 正哉; 益田 俊博; 二木 史朗

  第66回日本生化学会近畿支部例会, 25 May 2019

  
• がん細胞由来のエクソソームを認識する曲率感知ペプチドの開発

  河野 健一; 小串 正哉; 益田 俊博; 二木 史朗

  日本薬学会第139年会, 21 Mar. 2019

  
• がん細胞由来のエクソソームを標的としたペプチドベースの認識ツール開発 －曲率感知ペプチドの選定と評価－

  河野 健一; 小串 正哉; 益田 俊博; 二木 史朗

  第68回 日本薬学会近畿支部総会・大会, 13 Oct. 2018

  
• 高曲率小胞指向性を有する曲率感知ペプチドの開発

  河野健一; 小串正哉; 益田俊博; 二木史朗

  第2回統合物質創製化学研究推進機構(IRCCS)若手の会, 15 Jun. 2018

  
• 脂質小胞を感知する曲率センターペプチドの開発

  河野健一; 小串正哉; 益田俊博; 二木史朗

  第65回日本生化学会近畿支部例会, 26 May 2018

  
• ステープルドペプチドの細胞内移行における疎水性の重要性

  坂上功樹; 益田俊博; 河野健一; 二木史朗

  日本薬学会第138年会, 26 Mar. 2018

  
• Lipid packing loosening plays a key role in the direct membrane translocation of octaarginine

  Tomo Murayama; ◯Kenichi Kawano; Shiroh Futaki

  The Second International Symposium on Biofunctional Chemistry 2017, 14 Dec. 2017

  
• Modification of Lipid Packing State by Amphipathic Peptides with Curvature Inducibility.

  Tomo Murayama; ◯Kenichi Kawano; Shiroh Futaki

  統合物質創製化学研究推進機構 第3回国内シンポジウム, 30 Oct. 2017

  
• 曲率誘導性を有する両親媒性ヘリックスペプチド によるオクタアルギニンの膜透過促進

  河野健一; 村山知; 益田俊博; 二木史朗

  第11回バイオ関連化学シンポジウム, 07 Sep. 2017

  
• 曲率誘導性を有する両親媒性ヘリックスペプチドによるオクタアルギニンの膜透過促進

  村山知; 河野健一; 二木史朗

  統合物質創製化学研究推進機構 (IRCCS) 第一回若手の会, 29 Jul. 2017

  
• Membrane-Remodeling Amphipathic Peptides Facilitate Direct Penetration of Octaarginine (R8)

  村山知; 河野健一; 二木史朗

  日本薬学会第137年会, 25 Mar. 2017

  
• STOICHIOMETRIC ANALYSIS OF OLIGOMERIC STATES OF THREE CLASS-A GPCRS, CHEMOKINE-CXCR4, DOPAMINE-D2, AND PROSTAGLANDIN-EP1 RECEPTORS, ON LIVING CELLS

  ◯Kenichi Kawano; Tetsuya Yagi; Yoshiaki Yano; Katsumi Matsuzaki

  The 53th Japanese Peptide Symposium, 27 Oct. 2016

  
• Cell penetration of dipicolylamine-modified R8 facilitated by metal complex formation

  Shoko Ise; Yoshimasa Kawaguchi; Toshihide Takeuchi; ○Kenichi Kawano; Shiroh Futaki

  34th European Peptide Symposium and 8th International Peptide Symposium, 08 Sep. 2016

  
• Facilitated cell penetration of dipicolylamine-modified R8 by metal complex formation

  Shoko Ise; Yoshimasa Kawaguchi; Toshihide Takeuchi; ◯Kenichi Kawano; Shiroh Futaki

  The 14th Chinese International Peptide Symposium & the 5th Asia-pacific International Peptide Symposium, 05 Jul. 2016

  
• 膜張力変化を介した細胞運動制御ペプチドの開発

  益田俊博; 村山知; 河野健一; 二木史朗

  統合物質創製化学研究推進機構MEXT-IRCCS, 23 Jun. 2016

  
• 新規小分子ラベル法とin-cell FRETを用いた膜タンパク質の会合状態の定量的解析

  河野健一; 矢野義明; 大前薫; 松崎紗矢香; 松崎勝巳

  第64回日本薬学会近畿支部総会・大会, 11 Oct. 2014, Invited

  
• 抗インフルエンザウイルス薬アマンタジン耐性M2 S31N変異株の生体膜中における会合状態

  河野健一; 矢野義明; 松崎勝巳

  日本薬学会第134年会, 28 Mar. 2014

  
• Dimer is a minimal functional unit for influenza A virus M2 channel on biomembrane

  ◯Kenichi Kawano; Yoshiaki Yano; Katsumi Matsuzaki

  4th Asia-Pacific International Peptide Symposium, 06 Nov. 2013

  
• 新規小分子ラベル法とin-cell FRETを用いた膜タンパク質の会合状態の定量的解析

  河野健一; 矢野義明; 大前薫; 松崎紗矢香; 松崎勝巳

  第63回日本薬学会近畿支部総会・大会, 12 Oct. 2013

  
• インフルエンザAウィルスM2プロトンチャネルの生体膜中における会合状態

  河野健一; 松崎紗矢香; 大前薫; 矢野義明; 松崎勝巳

  第34回生体膜と薬物の相互作用シンポジウム, 15 Nov. 2012

  
• Mechanism of antiviral Amantadine resistance of influenza A virus M2 proton channel

  ◯Kenichi Kawano; Sayaka Matsuzaki; Kaoru Oomae; Yoshiaki Yano; Katsumi Matsuzaki

  49th Japanese Peptide Symposium, 07 Nov. 2012

  
• インフルエンザAウィルスM2プロトンチャネルの抗ウィルス薬 アマンタジン耐性メカニズム

  河野健一; 松崎紗矢香; 大前 薫; 矢野義明; 松崎勝巳

  第10回次世代を担う若手のフィジカル・ファーマフォーラム（PPF2012）, 06 Aug. 2012

  
• Stoichiometric analysis of oligomerization of membrane proteins on living cell surface

  ◯Kenichi Kawano; Kaoru Oomae; Sayaka Matsuzaki; Yoshiaki Yano; Katsumi Matsuzaki

  The 7th Seoul-Kyoto-Osaka Joint Symposium on Pharmaceutical Sciences for Young Scientists, 24 May 2012

  
• M2プロトンチャネルのpH依存的会合状態の変化

  河野健一; 大前薫; 松崎紗矢香; 矢野義明; 松崎勝巳

  日本薬学会第132年会, 30 Mar. 2012

  
• Family A GPCRの受容体活性に会合体形成は本当に必要か

  河野 健一

  第33回生体膜と薬物の相互作用シンポジウム, 24 Nov. 2011

  
• Family A GPCR の受容体活性に会合体形成は本当に必要か

  河野健一; 大前薫; 矢野義明; 松崎勝巳

  第9回次世代を担う若手のフィジカル・ファーマフォーラム（PPF2011）, 13 Sep. 2011

  
• 新規小分子蛍光ラベル法を用いたβ2アドレナリン受容体の生体膜における会合状態の解析

  河野健一; 矢野義明; 松崎勝巳

  日本ケミカルバイオロジー学会第６回年会, 25 May 2011

  

Books and Other Publications

• 細胞外小胞を利用した利己的ながん細胞の生存戦略と技術革新

  河野健一, Single work

  生物工学会誌「バイオミディア」第102巻 第11号, Nov. 2024, Refereed

  
• ペプチドと生体膜研究、そして分光学との出会い

  河野健一, Single work

  日本薬学会 ファルマシア60巻3号, Mar. 2024, Refereed

  
• ペプチドが切り拓く小胞研究の新世界 〜マーカー発現量に依存しない新たなエクソソーム検出法の開発〜

  河野健一, Single work

  月刊「化学」76巻4号 Review of Chemistry, 01 Apr. 2021, Refereed

  
• 中分子創薬研究のフロンティア －生体分子を標的とした機能性ペプチドの創製

  河野健一; 中瀬生彦, Joint work

  日本薬学会 薬事日報, 19 Mar. 2021, Refereed

  
• 中分子創薬研究のフロンティア:~ 生体分子の反応集積場としての膜曲率の役割~

  河野健一; 中瀬生彦, Joint work

  日本薬学会 薬事日報, 18 Mar. 2020, Refereed

  
• 細胞膜透過性（医療・診断をささえるペプチド科学―再生医療・DDS・診断への応用―）

  二木史朗; 秋柴美沙穂; 河野健一, Joint work

  シーエムシー出版, 30 Oct. 2017, Refereed

  

Awards

• 12 Oct. 2013

  日本薬学会近畿支部, 第63回日本薬学会近畿支部奨励賞

Media Coverage

• Extracellular-vesicle catch-and-release isolation system using a net-charge invertible curvature-sensing peptide

  Kyoto University, 26 Feb. 2024, Internet

  
• 脂質パッキングのゆるみが膜透過ペプチドの細胞内移行を促進する

  京都大学化学研究所, 09 Jun. 2017, Internet

  

External funds: Kakenhi

• Exploration of adjuvant-active substances derived from extracellular membrane vesicles

  Grant-in-Aid for Early-Career Scientists

  Basic Section 47030:Pharmaceutical hygiene and biochemistry-related

  Kyoto University

  河野 健一

  From 01 Apr. 2021, To 31 Mar. 2024, Granted

  細胞外小胞;曲率認識ペプチド;超遠心分離法;腸内細菌;アジュバント;免疫細胞;蛍光イメージング

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K15254/
• In-cell分光法によるアミロイドβ産生制御機構・構造変化の解明と創薬

  Grant-in-Aid for Scientific Research (B)

  Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related

  Kyoto University

  松崎 勝巳

  From 01 Apr. 2021, To 31 Mar. 2025, Granted

  アルツハイマー病

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H02615/
• Elucidation of the mechanism underlying the influx point of cell-penetrating peptides using artificial lipid domains

  Grant-in-Aid for Early-Career Scientists

  Basic Section 43040:Biophysics-related

  Kyoto University

  Kenichi Kawano

  From 01 Apr. 2018, To 31 Mar. 2020, Project Closed

  人工脂質ラフト;錯体脂質;細胞膜;境界領域;錯体化学;膜透過性ペプチド;直接膜透過;脂質パッキング

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K14664/
• Development of a novel fluorescent labeling method for detecting heterooligomers of membrane proteins on living cells

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Matsuzaki Katsumi

  From 01 Apr. 2015, To 31 Mar. 2018, Project Closed

  蛍光イメージング;膜タンパク質

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15H04636/
• New strategy for intracellular delivery of biomacropharmaceuticals

  Grant-in-Aid for Scientific Research (A)

  Kyoto University

  Shiroh Futaki

  From 01 Apr. 2015, To 31 Mar. 2018, Project Closed

  細胞内送達;抗体;エンドサイトーシス;マクロピノサイトーシス;クモ毒;膜傷害性ペプチド;バイオ医薬品;バイオ高分子医薬品;エンドソーム膜不安定化ペプチド;薬学;蛋白質;細胞;薬物送達

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15H02497/
• Structural analysis of Am-resistant M2-S31N mutant using a novel detergent and solution NMR

  Grant-in-Aid for Research Activity Start-up

  Kyoto University

  Kenichi Kawano

  From 29 Aug. 2014, To 31 Mar. 2016, Project Closed

  可溶化剤;M2プロトンチャネル;S31N変異体;溶液NMR;A型インフルエンザウイルス;アマンタジン耐性;二量体

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26893117/
• 新規リン脂質誘導体可溶化剤を用いた革新的膜タンパク質研究プラットホームの構築

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 47:Pharmaceutical sciences and related fields

  Kyoto University

  松崎 勝巳

  From 30 Jun. 2023, To 31 Mar. 2025, Granted

  膜タンパク質;可溶化剤

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K18184/
• Exploration for functional factors governing the diversity of extracellular vesicles: Single-cell analysis

  Grant-in-Aid for Scientific Research (C)

  Basic Section 47030:Pharmaceutical hygiene and biochemistry-related

  Kyoto University

  河野 健一

  From 01 Apr. 2024, To 31 Mar. 2027, Granted

  細胞外小胞;マイクロ流路デバイス;曲率認識ペプチド;オートインデューサー;1細胞解析

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K09777/
• In-cell分光法によるアミロイドβ産生制御機構・構造変化の解明と創薬

  Grant-in-Aid for Scientific Research (B)

  Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related

  Kyoto University

  松崎 勝巳

  From 01 Apr. 2021, To 31 Mar. 2025, Granted

  アルツハイマー病;アミロイドβ

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K21355/

External funds: others

• がん細胞由来のエクソソームを標的としたペプチド転移診断薬の開発

  武田科学振興財団 2018年度薬学系研究助成

  From 13 Nov. 2018, To 31 May 2021

  河野健一

Education

Teaching subject(s)

• From 01 Apr. 2024, To 31 Mar. 2025

  Basic Physical Chemistry (thermodynamics)

  N340, Fall, Institute for Liberal Arts and Sciences, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Laboratory Practice in Basic Analytical Chemistry and Structural Biology

  4202, Year-long, Graduate School of Pharmaceutical Sciences, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Physical Chemistry for Drug Discovery B

  8202, Spring, Graduate School of Pharmaceutical Sciences, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Pharmaceutical Laboratory I : Analytical and Physical Chemistry

  C901, Spring, Faculty of Pharmaceutical Sciences, 3
• From 01 Apr. 2024, To 31 Mar. 2025

  Research in Basic Biophysical Chemistry

  3202, Year-long, Graduate School of Pharmaceutical Sciences, 4
• From 01 Apr. 2024, To 31 Mar. 2025

  Introduction to Physical Chemistry for Drug Discovery

  8101, Spring, Graduate School of Pharmaceutical Sciences, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Pharmaceutical Sciences II

  6006, Spring, Graduate School of Pharmaceutical Sciences, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Introduction to Pharmaceutical Sciences

  6002, Spring, Graduate School of Pharmaceutical Sciences, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar in Basic Biophysical Chemistry

  2302, Year-long, Graduate School of Pharmaceutical Sciences, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Introduction to Physical Chemistry for Drug Discovery

  1217, Spring, Graduate School of Pharmaceutical Sciences, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Basic Physical Chemistry II

  1212, Spring, Graduate School of Pharmaceutical Sciences, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Basic Physical Chemistry (thermodynamics)

  N340, Fall, Institute for Liberal Arts and Sciences, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Laboratory Practice in Basic Analytical Chemistry and Structural Biology

  4202, Year-long, Graduate School of Pharmaceutical Sciences, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Pharmaceutical Laboratory I : Analytical and Physical Chemistry

  C901, Spring, Faculty of Pharmaceutical Sciences, 3
• From 01 Apr. 2023, To 31 Mar. 2024

  Research in Basic Biophysical Chemistry

  3202, Year-long, Graduate School of Pharmaceutical Sciences, 4
• From 01 Apr. 2023, To 31 Mar. 2024

  Pharmaceutical Laboratory 1 : Analytical and Physical Chemistry

  A901, Spring, Faculty of Pharmaceutical Sciences, 4
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar in Basic Biophysical Chemistry

  2302, Year-long, Graduate School of Pharmaceutical Sciences, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Introduction to Physical Chemistry for Drug Discovery

  1217, Spring, Graduate School of Pharmaceutical Sciences, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Introduction to Physical Chemistry for Drug Discovery

  8101, Spring, Graduate School of Pharmaceutical Sciences, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Introduction to Pharmaceutical Sciences

  6002, Spring, Graduate School of Pharmaceutical Sciences, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Introduction to Physical Chemistry for Drug Discovery

  1217, Spring, Graduate School of Pharmaceutical Sciences, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Laboratory Practice in Basic Analytical Chemistry and Structural Biology

  4202, Year-long, Graduate School of Pharmaceutical Sciences, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Introduction to Physical Chemistry for Drug Discovery

  8101, Spring, Graduate School of Pharmaceutical Sciences, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Research in Basic Biophysical Chemistry

  3202, Year-long, Graduate School of Pharmaceutical Sciences, 4
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar in Basic Biophysical Chemistry

  2302, Year-long, Graduate School of Pharmaceutical Sciences, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Basic Physical Chemistry (thermodynamics)

  N340, Fall, Institute for Liberal Arts and Sciences, 2
• From Apr. 2017, To Mar. 2018

  Laboratory Practice in Basic Biofunctional Chemistry

  Year-long, 薬学研究科
• From Apr. 2017, To Mar. 2018

  Research in Basic Biofunctional Chemistry

  Year-long, 薬学研究科
• From Apr. 2017, To Mar. 2018

  Basic Biofunctional Chemistry Seminar

  Year-long, 薬学研究科
• From Apr. 2018, To Mar. 2019

  Laboratory Practice in Basic Biofunctional Chemistry

  Year-long, 薬学研究科
• From Apr. 2018, To Mar. 2019

  Research in Basic Biofunctional Chemistry

  Year-long, 薬学研究科
• From Apr. 2018, To Mar. 2019

  Basic Biofunctional Chemistry Seminar

  Year-long, 薬学研究科
• From Apr. 2019, To Mar. 2020

  Laboratory Practice in Basic Biofunctional Chemistry

  Year-long, 薬学研究科
• From Apr. 2019, To Mar. 2020

  Research in Basic Biofunctional Chemistry

  Year-long, 薬学研究科
• From Apr. 2019, To Mar. 2020

  Basic Biofunctional Chemistry Seminar

  Year-long, 薬学研究科
• From Apr. 2020, To Mar. 2021

  Laboratory Practice in Basic Biofunctional Chemistry

  Year-long, 薬学研究科
• From Apr. 2020, To Mar. 2021

  Research in Basic Biofunctional Chemistry

  Year-long, 薬学研究科
• From Apr. 2020, To Mar. 2021

  Basic Biofunctional Chemistry Seminar

  Year-long, 薬学研究科

Administration

Faculty management (title, position)

• From 01 Apr. 2022, To 31 Mar. 2025

  環境・安全衛生委員会　委員
• From 01 Apr. 2016, To 31 Mar. 2017

  化学研究所情報システム技術委員会 委員
• From 01 Apr. 2017, To 31 Mar. 2018

  化学研究所安全衛生委員会 委員
• From 01 Apr. 2017, To 31 Mar. 2018

  化学研究所情報システム技術委員会 委員
• From 01 Apr. 2018, To 31 Mar. 2019

  化学研究所情報システム技術委員会 委員
• From 01 Apr. 2019, To 31 Mar. 2020

  化学研究所情報システム技術委員会 委員
• From 01 Apr. 2020, To 31 Mar. 2021

  化学研究所広報委員会委員
• From 01 Apr. 2020, To 31 Mar. 2021

  化学研究所情報システム技術委員会委員

Academic, Social Contribution

Committee Memberships

• From Apr. 2023, To Mar. 2025

  第75, 76号薬友会誌 編集員, 京都大学薬友会
• From Mar. 2020, To Jul. 2022

  関西支部幹事, 生体関連化学部会 若手の会
• From Aug. 2018, To Present

  幹事, 若手ペプチド夏の勉強会
• From Jul. 2019, To Oct. 2020

  実行委員・事務局, 第42回生体膜と薬物の相互作用シンポジウム(2020)

Academic Contribution

• 第12回バイオ関連化学シンポジウム若手フォーラム（世話人）

  Planning, management, etc.

  日本化学会 生体機能関連化学部会, From 01 Sep. 2025

  
• 日本膜学会 第47年会（実行委員）

  Planning, management, etc.

  日本膜学会, From 04 Jun. 2025, To 05 Jun. 2025

  
• 日本膜学会第46年会 生体膜シンポジウム企画（世話人代表）

  Planning, management, etc.

  日本膜学会, From 12 Jun. 2024

  
• 第42回生体膜と薬物の相互作用シンポジウム(2020)（実行委員・事務局）

  Planning, management, etc.

  日本薬学会 物理系薬学部会, From 28 Oct. 2021, To 29 Oct. 2021

  
• 第20回次世代を担う若手のためのフィジカル・ファーマフォーラム（PPF2023）（世話人）

  Planning, management, etc.

  日本薬学会 物理系薬学部会, From 31 Aug. 2023, To 01 Sep. 2023

  
• The 55th Summer Seminar for Young Peptide Scientist

  Planning, management, etc.

  The Japanese Peptide Society, From 08 Aug. 2023, To 10 Aug. 2023

  
• 生体機能関連化学部会若手の会 第33回サマースクール（実行委員長）

  Planning, management, etc.

  日本化学会 生体機能関連化学部会 若手の会, From 13 Jul. 2022, To 14 Jul. 2022

  
• 日本薬学会第141年会 シンポジウム企画（世話人代表）

  Planning, management, etc.

  日本薬学会, From 29 Mar. 2021

  
• 日本薬学会第140年会 シンポジウム企画（世話人代表）

  Planning, management, etc.

  日本薬学会, From 27 Mar. 2020

  

Social Contribution

• 京都大学オープンキャンパス2024

  Logistic support, Performer

  京都大学, From 08 Aug. 2024, To 09 Aug. 2024

  
• ELCAS（高校生のための体験型学習講座） 〜07.医薬品の細胞内送達の科学〜

  Host, Lecturer, Consultant, Official expert, Planner

  京都大学, From 16 Oct. 2021, To 13 Nov. 2021

  
• 日本ペプチド学会市民フォーラム2018（世話人代表）

  Host, Chief editor, Edit, Planner, Logistic support

  日本ペプチド学会, 自然に息づく万能素材「アミノ酸・ペプチド」 ~いのちを支え健康を守る立役者~, From 05 Dec. 2018