Sugase, Kenji(Graduate School of Agriculture, Division of Applied Life Sciences) | Activity Database on Education and Research, Kyoto University

Sugase, Kenji

Graduate School of Agriculture, Division of Applied Life Sciences Professor

Last Updated :2026/06/26

Basic Information

Faculty

Faculty of Agriculture

Professional Memberships

From Jun. 2023, To Present
日本農芸化学会

From 2021, To Present
日本生物物理学会

From 2008, To Present
The Nuclear Magnetic Resonance Society of Japan

From 2008, To Present
PROTEIN SCIENCE SOCIETY OF JAPAN

Academic Degree

Master of Engineering(Yokohama National University)

Doctor of Engineering (Yokohama National University)

Research History

From Apr. 2022, To Present
Kyoto University, Division of Applied Life Sciences, Graduate School of Agriculture, Full Professor

From Feb. 2015, To Mar. 2022
Kyoto University, Department of Molecular Engineering, Graduate School of Engineering, Associate Professor

From Apr. 2018, To Mar. 2019
Osaka University, Institute for Protein Research, 客員フェロー

From Jan. 2011, To Jan. 2015
Suntory Foundation for Life Sciences, Bioorganic Research Institute, NMR Group Leader, Senior Researcher

From Apr. 2009, To Jan. 2015
Kobe University, Graduate School of Engineering, Visiting Associate Professor

From Oct. 2008, To Dec. 2010
Suntory Bioorganic Research Institute, NMR Group Leader, Senior Researcher

From Apr. 2008, To Sep. 2008
Suntory Bioorganic Research Institute, Senior Researcher

From Apr. 1997, To Mar. 2008
Suntory Bioorganic Research Institute, Researcher

From Sep. 2005, To Aug. 2006
The Scripps Research Institute, Department of Molecular Biology, Senior Research Associate

From Sep. 2003, To Aug. 2005
The Scripps Research Institute, Department of Molecular Biology, Research Associate

Language of Instruction

English

Japanese

Last Updated :2026/06/26

Research

Research Topics, Overview of the research

Research Topics
Mechanism of protein aggregation in dynamic solution environments
Mechanism of protein fibrillation inhibition by food ingredients
Mechanism of long term memory formation by aggregation-coupled protein translation
Overview of the research
The in vivo environment is a dynamic solution environment in which blood flow and the electric field of neurons exist. Recently, it has been shown that certain proteins undergo liquid-liquid phase separation and amyloid fibrillation in response to the dynamic solution environments. Liquid-liquid phase separation and amyloid fibrillation are associated with long-term memory formation and neurodegenerative diseases. I have been studying the mechanisms of liquid-liquid phase separation and amyloid fibrillation that form in response to the dynamic solution environment, and also studying the mechanism of inhibition of amyloid fibrosis by food ingredients.

Research Interests

E-NMR

Rheo-NMR

protein aggregation

Biophysics

Structural Biology

Papers

Leucyl-tRNA Synthetase as a Molecular Target of Isobutanol-Mediated Growth Inhibition in Saccharomyces cerevisiae
Mano Hasegawa; Nodoka Oshimura; Kaho Hitomi; Ayako Furukawa; Kenji Sugase; Kouichi Kuroda
Journal of Biological Chemistry, Jun. 2026, Peer-reviewed, Corresponding author

Backbone resonance assignments of CPEB3 [1–120], CPEB3 [186–315], and CPEB3 [400–459]
Yujin Lee; Harunobu Saito; Masatomo So; Ayako Furukawa; Kenji Sugase
Biomolecular NMR Assignments, 29 May 2026, Peer-reviewed, Last author, Corresponding author

The Effect of Protein Tagging on Aggregation and Phase Separation
Harunobu Saito; Kenji Sugase
Journal of Cellular Biochemistry, 14 Jun. 2026, Peer-reviewed, Invited, Last author, Corresponding author

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
Ryuichi Hyakumoto; Masatomo So; Ayako Furukawa; Kenji Sugase
FEBS Letters, 20 May 2026, Peer-reviewed, Last author, Corresponding author

Mechanically stable high-sensitivity Rheo-NMR spectroscopy for real-time monitoring of shear-induced phase transitions
Akihiko Yamamoto; Yusuke Shuto; Daichi Morimoto; Ulrich Scheler; Ayako Furukawa; Masatomo So; Kenji Sugase
2026, Peer-reviewed, Invited, Last author, Corresponding author

Moiety-specific mechanism of ATP's hydrotropic action on α-synuclein
Yusuke Shuto; Toshifumi Mori; Benjamin Kohn; Erik Walinda; Daichi Morimoto; Ulrich Scheler; Masatomo So; Ayako Furukawa; Norio Yoshida; Kenji Sugase
Physical Chemistry Chemical Physics, 2026, Peer-reviewed, Last author, Corresponding author

1H, 13C, and 15N backbone resonance assignments of the A2 domain of human von Willebrand factor
Ryuichi Hyakumoto; Masatomo So; Ayako Furukawa; Daichi Morimoto; Kenji Sugase
Biomolecular NMR Assignments, 23 Mar. 2026, Peer-reviewed, Last author, Corresponding author
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Micelle and Inverse Micelle Structure Driven Viscoelasticity and Phase Separation in 2-Isobutoxyethanol–Water Mixtures: Insights from All-Atom Simulations
Mayank Dixit; Kenji Sugase; Takashi Taniguchi
Precision Chemistry, 08 Dec. 2025, Peer-reviewed, Corresponding author

Development of a Real-Time Monitoring System for the Adherent Human Keratinocyte Metabolism by Live-Cell Metabolic NMR with Illumination
Makoto Uyama; Yuki Umino; Fumio Matsuda; Ayako Furukawa; Kenji Sugase; Takahisa Ikegami
Analytical Chemistry, 09 Oct. 2025, Peer-reviewed

Backbone resonance assignments of the CPEB3 [101–200] and CPEB3 [294–410]
Harunobu Saito; Yujin Lee; Motoharu Ueno; Naotaka Sekiyama; Masatomo So; Ayako Furukawa; Kenji Sugase
Biomolecular NMR Assignments, 29 Mar. 2025, Peer-reviewed, Last author, Corresponding author
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Purification tags markedly affect self‐aggregation of CPEB3
Harunobu Saito; Yujin Lee; Motoharu Ueno; Naotaka Sekiyama; Masatomo So; Ayako Furukawa; Kenji Sugase
FEBS Letters, 17 Jun. 2025, Peer-reviewed, Last author, Corresponding author
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Autonomous ribosome biogenesis in vitro.
Yuishin Kosaka; Yumi Miyawaki; Megumi Mori; Shunsuke Aburaya; Chisato Nishizawa; Takeshi Chujo; Tatsuya Niwa; Takumi Miyazaki; Takashi Sugita; Mao Fukuyama; Hideki Taguchi; Kazuhito Tomizawa; Kenji Sugase; Mitsuyoshi Ueda; Wataru Aoki
Nature communications, 08 Jan. 2025, Peer-reviewed
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Evolutionary aspects of molecular forms and biological functions of oxytocin family peptides
Tsuyoshi Kawada; Toshio Sekiguchi; Kenji Sugase; Atsuhiro Kanda; Honoo Satake
Handbook of Oxytocin Research: Synthesis, Storage and Release, Actions and Drug Forms, Apr. 2009

Optimizing in vitro expression balance of central dogma-related genes using parallel reaction monitoring
Chisato Nishizawa; Shunsuke Aburaya; Yuishin Kosaka; Kenji Sugase; Wataru Aoki
Journal of Bioscience and Bioengineering, May 2024, Peer-reviewed

Applications of relaxation dispersion spectroscopy
Sugase, K.
Seikagaku, 2008

Structural analysis of 13C, 15N labeled adenosine by solid-state NMR.
Fujiwara, T.; Sugase, K.; Kainosyo, M.; Ono, A.; Akutsu, H.
Nucleic acids symposium series, 1995

An integrated approach of NMR experiments and MD simulations visualizes structural dynamics of a cyclic multi‐domain protein
Tomoki Sorada; Erik Walinda; Masahiro Shirakawa; Kenji Sugase; Daichi Morimoto
Protein Science, 15 Sep. 2023, Peer-reviewed

Solution structure of the HOIL-1L NZF domain reveals a conformational switch regulating linear ubiquitin affinity
Erik Walinda; Kenji Sugase; Naoki Ishii; Masahiro Shirakawa; Kazuhiro Iwai; Daichi Morimoto
Journal of Biological Chemistry, Aug. 2023, Peer-reviewed

Conformational Fluctuations and Induced Orientation of a Protein, Its Solvation Shell, and Bulk Water in Weak Non-Unfolding External Electric Fields
Yusuke Shuto; Erik Walinda; Daichi Morimoto; Kenji Sugase
The Journal of Physical Chemistry B, 16 Aug. 2023, Peer-reviewed, Last author, Corresponding author
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Crowded environments affect structural fluctuations and abnormal aggregation of proteins
森本大智; Erik Walinda; 菅瀬謙治
月刊「細胞」, Mar. 2023, Peer-reviewed, Invited, Corresponding author

Rheo‐NMR Spectroscopy for Cryogenic‐Probe‐Equipped NMR Instruments to Monitor Protein Aggregation
Daichi Morimoto; Erik Walinda; Akihiko Yamamoto; Ulrich Scheler; Kenji Sugase
Current Protocols, Dec. 2022, Peer-reviewed, Invited
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S03A3 Metastable structure detected by relaxation dispersion NMR spectroscopy(Visualising Dynamical Aspects of A Protein Molecule)
Sugase Kenji; Dyson Jane H.; Wright Peter E.
Seibutsu Butsuri, 2007

Counter-flow phenomena studied by nuclear magnetic resonance (NMR) velocimetry and flow simulations
Benjamin Kohn; Erik Walinda; Kenji Sugase; Daichi Morimoto; Ulrich Scheler
Physics of Fluids, Jul. 2022, Peer-reviewed

1SB-02 Identification of an allosteric site from the analysis of protein fluctuation using NMR(1SB Exploring design principles of life,The 49th Annual Meeting of the Biophysical Society of Japan)
Erisa Harada; Masakazu Sugishima; Jiro Harada; Masato Noguchi; Keiichi Fukuyama; Kenji Sugase
Seibutsu Butsuri, 2011, Last author, Corresponding author

1H1448 A design strategy of high-energy states of proteins based on high pressure NMR study(Protein: Property 2,The 49th Annual Meeting of the Biophysical Society of Japan)
Souichiro Kitazawa; Maho Yagi; Kenji Sugase; Koichi Kato; Ryo Kitahara
Seibutsu Butsuri, 2011

2P050 1E1435 The effect of structural dynamics of the Human Leucocyte Antigen on the function of cytotoxic T Lymphocyte(The 48th Annual Meeting of the Biophysical Society of Japan)
Saeko Yanaka; Kenji Sugase; Chihiro Motozono; Takamasa Ueno; Kouhei Tsumoto
Seibutsu Butsuri, 2010

1SC0945 Fluctuation and Function of Nuclear Pproteins(1SC Fluctuation and Function of Biomolecules,The 48th Annual Meeting of the Biophysical Society of Japan)
Kenji Sugase; Erisa Harada
Seibutsu Butsuri, 2010, Lead author, Corresponding author

Expression, solubility monitoring, and purification of the co-folded LUBAC LTM domain by structure-guided tandem folding in autoinducing cultures.
Erik Walinda; Daichi Morimoto; Tomoki Sorada; Kazuhiro Iwai; Kenji Sugase
Protein Expression and Purification, Nov. 2021, Peer-reviewed, Last author

Effects of Weak Nonspecific Interactions with ATP on Proteins.
Mayu Nishizawa; Erik Walinda; Daichi Morimoto; Benjamin Kohn; Ulrich Scheler; Masahiro Shirakawa; Kenji Sugase
Journal of the American Chemical Society, 11 Aug. 2021, Peer-reviewed, Corresponding author
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Multiple-State Monitoring of SOD1 Amyloid Formation at Single-Residue Resolution by Rheo-NMR Spectroscopy.
Naoto Iwakawa; Daichi Morimoto; Erik Walinda; Masahiro Shirakawa; Kenji Sugase
Journal of the American Chemical Society, 21 Jul. 2021, Peer-reviewed, Corresponding author

Backbone resonance assignments of the A2 domain of mouse von Willebrand factor.
Daichi Morimoto; Masanori Osugi; Yutaka Mahana; Erik Walinda; Masahiro Shirakawa; Kenji Sugase
Biomolecular NMR assignments, 15 Oct. 2021, Peer-reviewed, Corresponding author

Molecular recognition and deubiquitination of cyclic K48-linked ubiquitin chains by OTUB1
Tomoki Sorada; Daichi Morimoto; Erik Walinda; Kenji Sugase
Biochemical and Biophysical Research Communications, 12 Jul. 2021, Peer-reviewed

Glycyrrhizin Derivatives Suppress Cancer Chemoresistance by Inhibiting Progesterone Receptor Membrane Component 1
Yasuaki Kabe; Ikko Koike; Tatsuya Yamamoto; Miwa Hirai; Ayaka Kanai; Ryogo Furuhata; Hitoshi Tsugawa; Erisa Harada; Kenji Sugase; Kazue Hanadate; Nobuji Yoshikawa; Hiroaki Hayashi; Masanori Noda; Susumu Uchiyama; Hiroki Yamazaki; Hirotoshi Tanaka; Takuya Kobayashi; Hiroshi Handa; Makoto Suematsu
Cancers, 29 Jun. 2021, Peer-reviewed

Transient Diffusive Interactions with a Protein Crowder Affect Aggregation Processes of Superoxide Dismutase 1 β-Barrel.
Naoto Iwakawa; Daichi Morimoto; Erik Walinda; Sarah Leeb; Masahiro Shirakawa; Jens Danielsson; Kenji Sugase
The Journal of Physical Chemistry B, 18 Mar. 2021, Peer-reviewed, Corresponding author

Structural Dynamic Heterogeneity of Polyubiquitin Subunits Affects Phosphorylation Susceptibility
Daichi Morimoto; Erik Walinda; Shingo Takashima; Mayu Nishizawa; Kazuhiro Iwai; Masahiro Shirakawa; Kenji Sugase
Biochemistry, 02 Mar. 2021, Peer-reviewed, Corresponding author

Structural dynamics of double-stranded DNA with epigenome modification.
Ayako Furukawa; Erik Walinda; Kyohei Arita; Kenji Sugase
Nucleic Acids Research, 25 Jan. 2021, Peer-reviewed, Corresponding author

Rigorous analysis of the interaction between proteins and low water-solubility drugs by qNMR-aided NMR titration experiments
Takuya Hirakawa; Erik Walinda; Daichi Morimoto; Kenji Sugase
Physical Chemistry Chemical Physics, 06 Oct. 2021, Peer-reviewed, Corresponding author
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Quantitative monitoring of ubiquitination/deubiquitination reaction cycles by 18O-incorporation.
Yuka Tanaka; Daichi Morimoto; Erik Walinda; Kenji Sugase; Masahiro Shirakawa
Biochemical and Biophysical Research Communications, 20 Aug. 2020, Peer-reviewed

剪断力により誘起される相分離の機構解明
菅瀬謙治; 関山直孝; 吉田紀生
豊田研究報告 = Reports of Toyota Physical and Chemical Research Institute, May 2020, Invited

Pinpoint analysis of a protein in slow exchange using F1F2-selective ZZ-exchange spectroscopy: assignment and kinetic analysis
Mayu Nishizawa; Erik Walinda; Daichi Morimoto; Kenji Sugase
Journal of Biomolecular NMR, May 2020, Peer-reviewed, Corresponding author

Conformational exchange in the potassium channel blocker ShK
Naoto Iwakawa; Nicola J. Baxter; Dorothy C. C. Wai; Nicholas J. Fowler; Rodrigo A. V. Morales; Kenji Sugase; Raymond S. Norton; Mike P. Williamson
Scientific Reports, 17 Dec. 2019, Peer-reviewed

Topics from special edition 高感度Rheo-NMRによるアミロイド線維化過程のその場観察
森本大智; 菅瀬謙治
細胞, Oct. 2019, Invited, Corresponding author

Structural and thermodynamic basis for the recognition of the substrate-binding cleft on hen egg lysozyme by a single-domain antibody.
Hiroki Akiba; Hiroko Tamura; Masato Kiyoshi; Saeko Yanaka; Kenji Sugase; Jose M. M. Caaveiro; Kouhei Tsumoto
Scientific Reports, 29 Oct. 2019, Peer-reviewed
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Visualizing protein motion in Couette flow by all-atom molecular dynamics.
Erik Walinda; Daichi Morimoto; Masahiro Shirakawa; Ulrich Scheler; Kenji Sugase
Biochimica et Biophysica Acta General Subjects, Feb. 2020, Peer-reviewed, Invited, Corresponding author

NMR resonance assignments of the NZF domain of mouse HOIL-1L free and bound to linear di-ubiquitin.
Naoki Ishii; Erik Walinda; Naoto Iwakawa; Daichi Morimoto; Kazuhiro Iwai; Kenji Sugase; Masahiro Shirakawa
Biomolecular NMR assignments, Apr. 2019, Peer-reviewed

Backbone and side-chain resonance assignments of the methyl-CpG-binding domain of MBD6 from Arabidopsis thaliana.
Naoto Iwakawa; Yutaka Mahana; Arina Ono; Izuru Ohki; Erik Walinda; Daichi Morimoto; Kenji Sugase; Masahiro Shirakawa
Biomolecular NMR assignments, Apr. 2019, Peer-reviewed

Overview of relaxation dispersion NMR spectroscopy to study protein dynamics and protein-ligand interactions.
Erik Walinda; Daichi Morimoto; Kenji Sugase
Current Protocol in Protein Science, Apr. 2018, Peer-reviewed, Invited, Corresponding author

Hydrogen-deuterium exchange profiles of polyubiquitin fibrils
Daichi Morimoto; Ryo Nishizawa; Erik Walinda; Shingo Takashima; Kenji Sugase; Masahiro Shirakawa
Polymers, 27 Feb. 2018, Peer-reviewed, Invited, Corresponding author
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Isolation and characterization of a minimal building block of polyubiquitin fibrils.
Daichi Morimoto; Erik Walinda; Mayo Shinke; Kenji Sugase; Masahiro Shirakawa
Scientific Reports, 09 Feb. 2018, Peer-reviewed

Resolving biomolecular motion and interactions by R2 and R1ρ relaxation dispersion NMR
Erik Walinda; Daichi Morimoto; Kenji Sugase
Methods, 15 Sep. 2018, Peer-reviewed, Invited, Corresponding author
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Elucidating functional dynamics by R1ρ and R2 relaxation dispersion NMR spectroscopy
Erik Walinda; Kenji Sugase
Experimental Approaches of NMR Spectroscopy: Methodology and Application to Life Science and Materials Science, 23 Nov. 2017, Peer-reviewed, Invited, Corresponding author

Real-Time Observation of the Interaction between Thioflavin T and an Amyloid Protein by Using High-Sensitivity Rheo-NMR
Naoto Iwakawa; Daichi Morimoto; Erik Walinda; Yasushi Kawata; Masahiro Shirakawa; Kenji Sugase
International Journal of Molecular Sciences, 28 Oct. 2017, Peer-reviewed, Invited, Corresponding author
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Elucidation of potential sites for antibody engineering by fluctuation editing
Saeko Yanaka; Yoshitaka Moriwaki; Kouhei Tsumoto; Kenji Sugase
Scientific Reports, 30 Aug. 2017, Peer-reviewed, Corresponding author

High-Sensitivity Rheo-NMR Spectroscopy for Protein Studies
Daichi Morimoto; Erik Walinda; Naoto Iwakawa; Mayu Nishizawa; Yasushi Kawata; Akihiko Yamamoto; Masahiro Shirakawa; Ulrich Scheler; Kenji Sugase
Analytical Chemistry, 18 Jul. 2017, Peer-reviewed, Corresponding author

Biological and Physicochemical Functions of Ubiquitylation Revealed by Synthetic Chemistry Approaches
Daichi Morimoto; Erik Walinda; Kenji Sugase; Masahiro Shirakawa
International Journal of Molecular Sciences, 27 May 2017, Peer-reviewed, Invited
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F1F2-selective NMR spectroscopy
Erik Walinda; Daichi Morimoto; Masahiro Shirakawa; Kenji Sugase
Journal of Biomolecular NMR, May 2017, Peer-reviewed, Corresponding author

Exploration of the Conformational Dynamics of Major Histocompatibility Complex Molecules
Saeko Yanaka; Kenji Sugase
Frontiers in Immunology, 29 May 2017, Peer-reviewed, Invited, Corresponding author
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Backbone resonance assignments of monomeric SOD1 in dilute and crowded environments
Naoto Iwakawa; Daichi Morimoto; Erik Walinda; Kenji Sugase; Masahiro Shirakawa
Biomolecular NMR Assignments, Apr. 2017, Peer-reviewed

Practical considerations for investigation of protein conformational dynamics by 15N R1ρ relaxation dispersion
Erik Walinda; Daichi Morimoto; Masahiro Shirakawa; Kenji Sugase
Journal of Biomolecular NMR, Mar. 2017, Peer-reviewed, Corresponding author

The helical propensity of the extracellular loop is responsible for the substrate specificity of Fe(III)-phytosiderophore transporters
Erisa Harada; Kenji Sugase; Kosuke Namba; Yoshiko Murata
FEBS Letters, Dec. 2016, Peer-reviewed

Ubiquitylation Directly Induces Fold Destabilization of Proteins
Daichi Morimoto; Erik Walinda; Harumi Fukada; Kenji Sugase; Masahiro Shirakawa
Scientific Reports, 19 Dec. 2016, Peer-reviewed
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Dynamics of the Extended String-Like Interaction of TFIIE with the p62 Subunit of TFIIH
Masahiko Okuda; Junichi Higo; Tadashi Komatsu; Tsuyoshi Konuma; Kenji Sugase; Yoshifumi Nishimura
Biophysical Journal, 06 Sep. 2016, Peer-reviewed

Use of glass capillaries to suppress thermal convection in NMR tubes in diffusion measurements
Takashi Iwashita; Tsuyoshi Konuma; Erisa Harada; Shoko Mori; Kenji Sugase
Magnetic Resonance in Chemistry, Sep. 2016, Peer-reviewed

Dual function of phosphoubiquitin in E3 activation of parkin
Erik Walinda; Daichi Morimoto; Kenji Sugase; Masahiro Shirakawa
Journal of Biological Chemistry, 05 Aug. 2016, Peer-reviewed

Efficient identification and analysis of chemical exchange in biomolecules by R-1 rho relaxation dispersion with Amaterasu
Erik Walinda; Daichi Morimoto; Mayu Nishizawa; Masahiro Shirakawa; Kenji Sugase
Bioinformatics, 15 Aug. 2016, Peer-reviewed, Corresponding author

Quantitative analysis of protein-ligand interactions by NMR
Ayako Furukawa; Tsuyoshi Konuma; Saeko Yanaka; Kenji Sugase
Progress in Nuclear Magnetic Resonance Spectroscopy, Aug. 2016, Peer-reviewed, Invited, Corresponding author
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Haem-dependent dimerization of PGRMC1/Sigma-2 receptor facilitates cancer proliferation and chemoresistance
Yasuaki Kabe; Takanori Nakane; Ikko Koike; Tatsuya Yamamoto; Yuki Sugiura; Erisa Harada; Kenji Sugase; Tatsuro Shimamura; Mitsuyo Ohmura; Kazumi Muraoka; Ayumi Yamamoto; Takeshi Uchida; So Iwata; Yuki Yamaguchi; Elena Krayukhina; Masanori Noda; Hiroshi Handa; Koichiro Ishimori; Susumu Uchiyama; Takuya Kobayashi; Makoto Suematsu
Nature Communications, Mar. 2016, Peer-reviewed
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Extracting protein dynamics information from overlapped NMR signals using relaxation dispersion difference NMR spectroscopy
Tsuyoshi Konuma; Erisa Harada; Kenji Sugase
Journal of Biomolecular NMR, Dec. 2015, Peer-reviewed, Last author, Corresponding author

Dynamic changes in CCAN organization through CENP-C during cell-cycle progression
Harsh Nagpal; Tetsuya Hori; Ayako Furukawa; Kenji Sugase; Akihisa Osakabe; Hitoshi Kurumizaka; Tatsuo Fukagawa
Molecular Biology of the Cell, 01 Nov. 2015, Peer-reviewed

Conformational propensities of intrinsically disordered proteins influence the mechanism of binding and folding
Munehito Arai; Kenji Sugase; H. Jane Dyson; Peter E. Wright
Proceedings of the National Academy of Sciences of the United States of America, Aug. 2015, Peer-reviewed

Backbone assignments of the apo and Zn(II) protoporphyrin IX-bound states of the soluble form of rat heme oxygenase-1
Erisa Harada; Masakazu Sugishima; Jiro Harada; Masato Noguchi; Keiichi Fukuyama; Kenji Sugase
Biomolecular NMR assignments, Apr. 2015, Peer-reviewed, Last author, Corresponding author

Distal regulation of heme binding of heme oxygenase-1 mediated by conformational fluctuations
Erisa Harada; Masakazu Sugishima; Jiro Harada; Keiichi Fukuyama; Kenji Sugase
Biochemistry, 20 Jan. 2015, Peer-reviewed, Last author, Corresponding author

Elucidation of Structural Dynamics Using Relaxation Dispersion NMR Spectroscopy
菅瀬謙治
分光研究, Jan. 2015, Peer-reviewed, Invited, Lead author, Last author, Corresponding author

Revealing the peptide presenting process of human leukocyte antigen through the analysis of fluctuation
Saeko Yanaka; Takamasa Ueno; Kouhei Tsumoto; Kenji Sugase
Biophysics, 2015, Peer-reviewed, Invited, Last author, Corresponding author

Solution Structure of the Ubiquitin-associated (UBA) Domain of Human Autophagy Receptor NBR1 and Its Interaction with Ubiquitin and Polyubiquitin
Erik Walinda; Daichi Morimoto; Kenji Sugase; Tsuyoshi Konuma; Hidehito Tochio; Masahiro Shirakawa
Journal of Biological Chemistry, 16 May 2014, Peer-reviewed

Solid-state NMR spectra of lipid-anchored proteins under magic angle spinning
Kaoru Nomura; Erisa Harada; Kenji Sugase; Keiko Shimamoto
Journal of Physical Chemistry B, 06 Mar. 2014, Peer-reviewed

Quantitative analysis of location- and sequence-dependent deamination by APOBEC3G using real-time NMR spectroscopy.
Ayako Furukawa; Kenji Sugase; Ryo Morishita; Takashi Nagata; Tsutomu Kodaki; Akifumi Takaori-Kondo; Akihide Ryo; Masato Katahira
Angewandte Chemie International Edition, 24 Feb. 2014, Peer-reviewed
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2P064 The Dynamic stabilization and peptide recognition mechanism of Human Leukocyte Antigen revealed by NMR relaxation dispersion analysis(01C. Protein: Property,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))
Yanaka Saeko; Sugase Kenji; Ueno Takamasa; Tsumoto Kouhei
Seibutsu Butsuri, 2014

1P035 Conformational Flexibility of the high-mobility group box domain of sox2 and its folding coupled with DNA binding(Protein: Structure & Function,Poster,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))
Erisa Harada; Tsuyoshi Konuma; Syoko Mori; Kenji Sugase
Seibutsu Butsuri, 2014, Last author, Corresponding author

Peptide-dependent Conformational Fluctuation Determines the Stability of the Human Leukocyte Antigen Class I Complex
Saeko Yanaka; Takamasa Ueno; Yi Shi; Jianxun Qi; George F. Gao; Kouhei Tsumoto; Kenji Sugase
Journal of Biological Chemistry, Aug. 2014, Peer-reviewed, Last author, Corresponding author

実践relaxation dispersion法
菅瀬謙治
NMR, Oct. 2013, Peer-reviewed, Invited, Lead author, Last author, Corresponding author

Fast and accurate fitting of relaxation dispersion data using the flexible software package GLOVE
Kenji Sugase; Tsuyoshi Konuma; Jonathan C. Lansing; Peter E. Wright
Journal of Biomolecular NMR, Jul. 2013, Peer-reviewed, Lead author

Solution structure of the Q41N variant of ubiquitin as a model for the alternatively folded N2 state of ubiquitin
Soichiro Kitazawa; Tomoshi Kameda; Maho Yagi-Utsumi; Kenji Sugase; Nicola J. Baxter; Koichi Kato; Michael P. Williamson; Ryo Kitahara
Biochemistry, 19 Mar. 2013, Peer-reviewed

緩和分散NMR法による動的構造解析
谷中冴子、菅瀬謙治
蛋白質科学会アーカイブ, 2013, Peer-reviewed, Invited, Last author, Corresponding author

The Monomer–Seed Interaction Mechanism in the Formation of the β2-Microglobulin Amyloid Fibril Clarified by Solution NMR Techniques
Kotaro Yanagi; Kazumasa Sakurai; Yuichi Yoshimura; Tsuyoshi Konuma; Young-Ho Lee; Kenji Sugase; Takahisa Ikegami; Hironobu Naiki; Yuji Goto
Journal of Molecular Biology, Sep. 2012, Peer-reviewed

1PT112 Solution stucture of the alternatively folded state of ubiquitin(The 50th Annual Meeting of the Biophysical Society of Japan)
Soichiro Kitazawa; Tomoshi Kameda; Maho Yagi; Kenji Sugase; Nicky Baxter; Koichi Kato; Michael P. Williamson; Ryo Kitahara
Seibutsu Butsuri, 2012

Boosting Protein Dynamics Studies Using Quantitative Nonuniform Sampling NMR Spectroscopy
Yoh Matsuki; Tsuyoshi Konuma; Toshimichi Fujiwara; Kenji Sugase
The Journal of Physical Chemistry B, 24 Nov. 2011, Peer-reviewed, Last author, Corresponding author

Elucidating slow binding kinetics of a protein without observable bound resonances by longitudinal relaxation NMR spectroscopy
Kenji Sugase
Journal of Biomolecular NMR, Jul. 2011, Peer-reviewed, Lead author, Last author, Corresponding author

Lipopolysaccharide induces raft domain expansion in membrane composed of a phospholipid-cholesterol-sphingomyelin ternary system
Kaoru Nomura; Masahide Maeda; Kenji Sugase; Shoichi Kusumoto
Innate Immunity, Jun. 2011

Calcitonin in a protochordate, Ciona intestinalis - the prototype of the vertebrate calcitonin/calcitonin gene-related peptide superfamily
Toshio Sekiguchi; Nobuo Suzuki; Nobuyuki Fujiwara; Masato Aoyama; Tsuyoshi Kawada; Kenji Sugase; Yoshiko Murata; Yuichi Sasayama; Michio Ogasawara; Honoo Satake
FEBS Journal, Aug. 2009, Peer-reviewed

Specific transporter for iron(III): Phytosiderophore complex involved in iron uptake by barley roots
Yoshiko Murata; Emiko Harada; Kenji Sugase; Kosuke Namba; Manabu Horikawa; Jian Feng Ma; Naoki Yamaji; Daisei Ueno; Kyosuke Nomoto; Takashi Iwashita; Shoichi Kusumoto
Pure and Applied Chemistry, 01 Jan. 2008

New NMR Technique to Probe Invisible Low-Populated States-Development of R2 Relaxation Dispersion Spectroscopy-
Kenji SUGASE
Seibutsu Butsuri, 2008, Peer-reviewed, Invited, Lead author, Last author, Corresponding author

Relaxation Dispersion 法の生化学への応用
菅瀬謙治
生化学, Aug. 2008, Peer-reviewed, Invited, Lead author, Last author, Corresponding author

Overexpression of post-translationally modified peptides in Escherichia coli by co-expression with modifying enzymes
Sugase, K.; L; es, M.A.; Wright, P.E.; Martinez-Yamout, M.
Protein Expression and Purification, Feb. 2008, Peer-reviewed, Lead author

Tailoring Relaxation Dispersion Experiments for Fast-Associating Protein Complexes
Kenji Sugase; Jonathan C. Lansing; H. Jane Dyson; Peter E. Wright
Journal of the American Chemical Society, 01 Nov. 2007, Peer-reviewed, Lead author

Structural element responsible for the Fe(III)-phytosiderophore specific transport by HvYS1 transporter in barley
Emiko Harada; Kenji Sugase; Kosuke Namba; Takashi Iwashita; Yoshiko Murata
FEBS Letters, 04 Sep. 2007, Peer-reviewed

Intrinsically disordered protein and encounter complex
菅瀬謙治
Protein, nucleic acid and enzyme, Aug. 2007, Peer-reviewed, Invited, Lead author, Last author, Corresponding author

Solution structure of agelenin, an insecticidal peptide isolated from the spider Agelena opulenta, and its structural similarities to insect-specific calcium channel inhibitors
Nahoko Yamaji; Kenji Sugase; Terumi Nakajima; Takafumi Miki; Minoru Wakamori; Yasuo Mori; Takashi Iwashita
FEBS Letters, 07 Aug. 2007, Peer-reviewed

レスベラトロールがメタボリックシンドロームを改善する?
菅瀬謙治
ファルマシア, Jul. 2007, Peer-reviewed, Invited, Lead author, Last author, Corresponding author

Mechanism of coupled folding and binding of an intrinsically disordered protein
Kenji Sugase; H. Jane Dyson; Peter E. Wright
Nature, Jun. 2007, Peer-reviewed, Lead author

Solution structure of IsTX, a male scorpion toxin from O-madagascariensis
Nahoko Yamaji; Li Dai; Kenji Sugase; Marta Andriantsiferana; Terumi Nakajima; Takashi Iwashita
Peptides 2004, Proceedings, 2005, Peer-reviewed

Solution structure of IsTX. A male scorpion toxin from Opisthacanthus madagascariensis (Ischnuridae)
Nahoko Yamaji; Li Dai; Kenji Sugase; Marta Andriantsiferana; Terumi Nakajima; Takashi Iwashita
European Journal of Biochemistry, 10 Sep. 2004, Peer-reviewed

Receptor-bound characteristics of MiniANP analyzed by NMR, MM/MD, and the use of unusual amino acids
K. Sugase; M. Horikawa; M. Ishiguro
Peptide Revolution: Genomics, Proteomics & Therapeutics, 2004, Peer-reviewed

Restriction of a Peptide Turn Conformation and Conformational Analysis of Guanidino Group Using Arginine-Proline Fused Amino Acids: Application to Mini Atrial Natriuretic Peptide on Binding to the Receptor
Kenji Sugase; Manabu Horikawa; Masako Sugiyama; Masaji Ishiguro
Journal of Medicinal Chemistry, 01 Jan. 2004, Peer-reviewed, Lead author

Structure–activity relationships for mini atrial natriuretic peptide by proline-Scanning mutagenesis and shortening of peptide backbone
Kenji Sugase; Yoshiaki Oyama; Katsuhiko Kitano; Hideo Akutsu; Masaji Ishiguro
Bioorganic and Medicinal Chemistry Letters, May 2002, Peer-reviewed, Lead author

Designing Analogues of Mini Atrial Natriuretic Peptide Based on Structural Analysis by NMR and Restrained Molecular Dynamics
Kenji Sugase; Yoshiaki Oyama; Katsuhiko Kitano; Takashi Iwashita; Toshimichi Fujiwara; Hideo Akutsu; Masaji Ishiguro
Journal of Medicinal Chemistry, 01 Feb. 2002, Peer-reviewed, Lead author

Designing Analogs of Mini Atrial Natriuretic Peptide Based on Structural Analysis by NMR and Restrained Molecular Dynamics
Sugase Kenji; Oyama Yoshiaki; Kitano Katsuhiko; Iwashita Takashi; Fujiwara Toshimichi; Akutsu Hideo; Ishiguro Masaji
Symposium on Chemical Information and Computer Sciences
Symposium on Structure-Activity Relationships, 2001, Lead author

Characterization of a Receptor-Bound Conformation of Mini Atrial Natriuretic Peptide
K. Sugase; Y. Oyama; K. Kitano; T. Iwashita; T. Fujiwara; H. Akutsu; M. Ishiguro
Biochemical Society Transactions, 01 Oct. 2000, Lead author

Synthesis of the cyclic heptapeptide Substance P antagonist, dihydro-WIN67689 and determination of the stereochemistry of the modified tyrosine moiety
Yuan-Qiang Li; Kenji Sugase; Masaji Ishiguro
Tetrahedron Letters, Dec. 1999, Peer-reviewed

13C-13C and 13C-15N Dipolar Correlation NMR of Uniformly Labeled Organic Solids for the Complete Assignment of Their 13C and 15N Signals: An Application to Adenosine
Toshimichi Fujiwara; Kenji Sugase; Masatsune Kainosho; Akira Ono; Akira Ono; Hideo Akutsu
Journal of the American Chemical Society, Nov. 1995, Peer-reviewed

Iron Acquisition Mechanism of Graminaceous Plants by Mugineic Acids
Murata Yoshiko; Namba Kosuke; Ma Jian Feng; Sugase Kenji; Horikawa Manabu; Iwashita Takashi; Kusumoto Shoichi
Symposium on the Chemistry of Natural Products, symposium papers, 01 Sep. 2009

Structural Insights into Methylated DNA Recognition by the Methyl-CpG Binding Domain of MBD6 from Arabidopsis thaliana
Yutaka Mahana; Izuru Ohki; Erik Walinda; Daichi Morimoto; Kenji Sugase; Masahiro Shirakawa
ACS Omega, 19 Jan. 2022, Peer-reviewed
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Misc.

シロイヌナズナ由来のタンパク質MBD6によるメチル化DNA認識の構造基盤
真鼻裕; 岩川直都; 尾野有菜; 大木出; ヴァリンダエリック; 森本大智; 菅瀬謙治; 白川昌宏
日本細胞生物学会大会(Web), 2019

シロイヌナズナのDNA結合タンパク質MBD6の構造解析
真鼻裕; 岩川直都; 尾野有菜; 大木出; WALINDA Erik; 森本大智; 菅瀬謙治; 白川昌宏
日本分子生物学会年会プログラム・要旨集(Web), 2018

ユビキチン化に伴うタンパク質構造不安定化
森本大智; WALINDA Erik; 菅瀬謙治; 深田はるみ; 曽友深; 蔭山俊; 蔭山俊; 星野大; 藤井高志; 土屋光; 佐伯泰; 有田恭平; 有吉眞理子; 杤尾豪人; 岩井一宏; 難波啓一; 難波啓一; 小松雅明; 小松雅明; 田中啓二; 白川昌宏
Abstracts. Annual Meeting of the NMR Society of Japan, 2014

ヒト主要組織適合複合体の揺らぎが安定性に与える影響について
谷中冴子; 菅瀬謙治; 上野貴将; 津本浩平
日本蛋白質科学会年会プログラム・要旨集, 2012

ヒト主要組織適合複合体の安定化機構に関する解析
谷中冴子; 菅瀬謙治; 上野貴将; 津本浩平
Abstracts. Annual Meeting of the NMR Society of Japan, 2012

ヒト主要組織適合複合体(HLA)の揺らぎが傷害性T細胞(CTL)に与える影響について
谷中冴子; 菅瀬謙治; 上野貴将; 津本浩平
日本蛋白質科学会年会プログラム・要旨集, 2011

ヒト主要組織適合複合体の動的構造と傷害性T細胞の活性の関連について
谷中冴子; 上野貴将; 本園千尋; 菅瀬謙治; 津本浩平
日本蛋白質科学会年会プログラム・要旨集, 2010

ヒト主要組織適合複合体の動的なペプチド認識および構造維持機構
谷中冴子; 菅瀬謙治; 上野貴将; 津本浩平
日本蛋白質科学会年会プログラム・要旨集, 2013

NMRを用いたヒト主要組織適合複合体の動的なペプチド認識及び構造維持機構の解明
谷中冴子; 上野貴将; YI Shi; QI Jianxun; GAO George; 津本浩平; 菅瀬謙治
Abstracts. Annual Meeting of the NMR Society of Japan, 2013

Characterization of a Receptor-Bound Conformation of Mini Atrial Natriuretic Peptide
SUGASE Kenji; OYAMA Yoshiaki; KITANO Katsuhiko; IWASHITA Takashi; FUJIWARA Toshimichi; AKUTSU Hideo; ISHIGURO Masaji
Peptide science : proceedings of the ... Japanese Peptide Symposium, 01 Mar. 2001

The deamination of APOBEC3G os controlled by the sliding-direction-dependent catalytic activity, and anti-prion activity of an RNA aptamer and its structural basis
Furukawa, A; Sugase, K; Morishita, R; Nagata, T; Takaori-Kondo, A; Ryo, A; Mashima, T; Nishikawa, F; Nishikawa, S; Kamatari, Y; Kuwata, K; Katahira, M
The 10th Korea-Japan Bilateral Symposium, 2013, Invited
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タンパク質の構造破綻とその関連疾患物性化学から臨床まで細胞内におけるユビキチン陽性タンパク質凝集体の形成機構の解明
森本大智; ヴァリンダ・エリック; 深田はるみ; 菅瀬謙治; 蔭山俊; 星野大; 難波啓一; 小松雅明; 田中啓二; 白川昌宏
日本生化学会大会プログラム・講演要旨集, Sep. 2016

細胞周期中のCCANの構成の動的な変化(Dynamic Change of CCAN organization during the cell cycle)
Nagpal Harsh; 堀哲也; Furukawa Ayako; 菅瀬謙治; 越阪部晃永; 胡桃坂仁志; 深川竜郎
日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, Dec. 2015

Deamination mechanism of an anti-HIV APOBEC3G coupled with sliding on single-stranded DNA as analyzed by real-time NMR monitoring method
K. Kanba; A. Furukawa; K. Sugase; T. Nagata; M. Katahira
The 41st International Symposium on Nucleic Acids Chemistry.Fukuoka, Nov. 2014

Real-time NMR monitoring of enzymatic reaction of anti-HIV protein, structure of anti-prion RNA aptamer and wood biomass analysis
Kanba, K; Mashima, T; Okamura, H; Furukawa, A; Sugase, K; Nishimura, H; Watanabe, T; Nagata, T; Katahira, M
The 5th Japan-Taiwan NMR symposium, Sep. 2014

Functional mechanism of an anti-HIV protein, APOBEC3G, as revealed by real-time NMR monitoring of its enzymatic reaction
K. Kanba; A. Furukawa; K. Sugase; R. Morishita; T. Nagata; M. Katahira
XXVIth International Conference on Magnetic Resonance in Biological Systems.Dallas, Aug. 2014

The Dynamic Peptide Recognition And Stabilization Mechanism Of Human Leukocyte Antigen B*35:01
Saeko Yanaka; Takamasa Ueno; Kouhei Tsumoto; Kenji Sugase
PROTEIN SCIENCE, Jul. 2014

Elucidation Of The Nonspecific DNA-Binding Mechanism Of The POU Homeodomain Using NMR
Tsuyoshi Konuma; Erisa Harada; Takashi Oda; Mamoru Sato; Kenji Sugase
PROTEIN SCIENCE, Jul. 2014

Functional mechanism of anti-HIV protein, APOBEC3G, elucidated by real-time monitoring of enzymatic reaction, and anti-prion activity of an RNA aptamer with its structural basis
K. Kanba; A. Furukawa; K. Sugase; R. Morishita; T. Nagata; M. Katahira
The 45th Annual Meeting of Korean Magnetic Resonance Society, Jul. 2014, Invited

ヘムオキシゲナーゼ分子表面に存在するF79の変異導入解析から見出された電子伝達機構
原田二朗; 原田英里砂; 東元祐一郎; 佐藤秀明; 杉島正一; 平順一; 福山恵一; 菅瀬謙治; 野口正人
日本生化学会大会プログラム・講演要旨集, Dec. 2012

抗HIV宿主因子 APOBEC3Gの位置依存的デアミネーション反応機構の解明
Furukawa, A; Sugase, K; Morishita, R; Nagata, T; Ryo, A; Takaori, A; Katahira, M
日本生物物理学会第50回年会.名古屋, Sep. 2012

Elucidation of the location-dependent deamination reaction mechanism of an anti-HIV factor,APOBEC3G
A. Furukawa; K. Sugase; R. Morishita; A. Ryo; A. akaori; T. Nagata; M. Katahira
XXV international Conference on Magnetic Resonance in Biological Systems，2012, Aug. 2012

Anti-prion activity and it strucural basis of RNAaptamer, and sliding-direction-dependent deaminase activity of anti-HIV enzyme
T. Mashima; A. Furukawa; K. Sugase; H. Fujiwara; F. Nishikawa; R. Morishita; A. Ryo; Y. Kamatari; T. Nagata; S. Nishikawa; K. Kuwata; M. Katahira
XXV international Conference on Magnetic Resonance in Biological Systems，2012.Lyon,2012.8.19-24., Aug. 2012

アミロイド線維形成時に現れる過渡的中間体のキャラクタリゼーション
柳浩太郎; 櫻井一正; 吉村優一; 小沼剛; LEE Young‐Ho; 菅瀬謙治; 池上貴久; 内木宏延; 後藤祐児
日本蛋白質科学会年会プログラム・要旨集, 31 May 2012

Sliding-direction-dependent activity of anti-HIV enzyme,and structural basis of anti-prion activity of RNA aptamer
Furukawa, A; Mashima, T; Sugase, K; Fujiwara, H; Nagata, T; Morishita, R; Ryo, A; Nishikawa, F; Nishikawa, S; Kamatari, KO; Kuwata, K; Katahira, M
Korea-Japan Bilateral NMR Symposium, 16 Mar. 2012

Design Strategy for High Free-Energy States of Proteins Based on High-Pressure NMR Study: Alternatively Folded Conformation of Ubiquitin
Ryo Kitahara; Soichiro Kitazawa; Maho Yagi-Utsumi; Akemi Ido; Makoto Urushitani; Kenji Sugase; Koichi Kato
BIOPHYSICAL JOURNAL, Jan. 2012

Real-time monitoring of the cytidine deamination along single-stranded DNA by an anti-HIV factor, APOBEC3G
Furukawa, A; Sugase, K; Morishita, R; Nagata, T; Ryo, A; Takaori, A; Katahira, M
The International Symposium on Nuclear Magnetic Resonance 2011, Nov. 2011

Base-deamination rate dependent on the direction of sliding of an enzyme along DNA as revealed by numerical analysis of NMR data, and wood biomass studied by solution NMR
NISHIMURA Hiroshi; FURUKAWA Ayako; OKAMURA Hideyasu; KOZAWA Yu; MORISHITA Ryo; RYO Akihide; SUGASE Kenji; WATANABE Takashi; KATAHIRA Masato
Abstr Annu Meet NMR Soc Jpn, 21 Oct. 2011, Peer-reviewed

ヘムオキシゲナーゼの変異体の解析から考察される電子伝達経路
原田二朗; 原田英里砂; 東元祐一郎; 佐藤秀明; 杉島正一; 平順一; 福山恵一; 菅瀬謙治; 野口正人
日本生化学会大会プログラム・講演要旨集, Sep. 2011

溶液NMRによるβ2ミクログロブリンアミロイド線維形成のモノマー‐線維核相互作用機構の解明
櫻井一正; 柳浩太郎; 吉村優一; 小沼剛; 菅瀬謙治; 池上貴久; 内木宏延; 後藤祐児
日本蛋白質科学会年会プログラム・要旨集, 27 May 2011

SOLUTION STRUCTURE OF ISTX, A MALE SCORPION TOXIN FROM O. MADAGASCARIENSIS
N. Yamaji; L. Dai; K. Sugase; M. Andriantsiferana; T. Nakajima; T. Iwashita
JOURNAL OF PEPTIDE SCIENCE, 2004

Receptor-bound characteristics of minianp analyzed by NMR, MM/MD, and the use of unusual amino acids
K Sugase; M Horikawa; M Ishiguro
BIOPOLYMERS, 2003

Multidimensional Solid-State NMR for the Structural Analysis of Isotope-Labeled Biomolecules
SASA K.; SUGASE K.; MORIKAWA S.; FUJIWARA T.; AKUTU H.
Biophysics, Oct. 1997

Presentations

Rheo-NMRを用いたα-シヌクレインのアミロイド線維化過程の速度論的解析
菅瀨謙治
第52回生体分子科学討論会, 15 May 2026, Invited

Rheo-NMR study on how green tea catechins suppress α-synuclein fibrillation
Kenji Sugase
Chile-Japan Academic Forum in Kyoto, 01 Oct. 2025, Invited

Rheo-NMRによる緑茶カテキンのα-シヌクレイン線維化抑制機構解明
菅瀨謙治
よこはまＮＭＲ研究会, 30 May 2025, Invited

Rheo-NMR 装置による生体高分子の動態解析
菅瀬謙治
食品ハイドロコロイドセミナー2025, 20 Jun. 2025, Invited

Site-Specific Kinetic Analysis of Protein Amyloid Fibrillation Under Flow Using High-Sensitivity Rheo-NMR
Kenji Sugase
6th International Symposium on Pathomechanisms of Amyloid Diseases, 06 Dec. 2024, Invited

F1F2-selective solution NMR spectroscopy
Kenji Sugase
Mini-symposium on the Recent Advances in NMR Spectroscopy, 04 Dec. 2024

Atomic-level Analysis of Proteins in Flow by Rheo-NMR
Kenji Sugase
The 3rd KU-UNIST Joint Symposium on Chemistry and Materials Science, 07 Nov. 2024, Invited

細胞内環境下での生体分子 NMR 解析を目指して：Rheo-NMR の挑戦
菅瀬謙治
第14回CSJ化学フェスタ2024, 24 Oct. 2024, Invited

NMR study of α-synuclein amyloid fibril formation and inhibition
Kenji Sugase
The 8th International Symposium on Drug Discovery and Design by NMR, 27 Aug. 2024, Invited

Site-Specific Kinetic Analysis of α-Synuclein Fibrillation by Using High-Sensitivity Rheo-NMR
Kenji Sugase
The 30th International Conference o n Magnetic Resonance in Biological Systems, 19 Aug. 2024, Invited

Rheo-NMRによるアミロイド線維化過程のリアルタイム計測
菅瀬謙治
第42回日本認知症学会学術集会, 25 Nov. 2023, Invited

動的溶液環境下のタンパク質の動的構造
菅瀬謙治
基礎から学ぶ最新NMR解析法第7回ワークショップタンパク質のダイナミクスと機能, 03 Oct. 2023, Invited

NMRによる動的溶液環境に応答する天然変性タンパク質の動的構造解析
菅瀬謙治
2022年度中性子構造生物学研究会「天然変性タンパク質」, 22 Mar. 2023, Invited

High-sensitivity Rheo-NMR
Kenji Sugase
The 7th International Symposium on Drug Discovery and Design by NMR, 27 Oct. 2022, Invited

Mechanism of amyloid fibrillation of a protein driven by flow-induced physical perturbation
Kenji Sugase
Joint Meeting of the 20th KIAS Conference on "Protein Structure and Function", 14 Sep. 2022, Invited

基礎講座
菅瀬謙治
第21回若手NMR研究会, Sep. 2021, Invited

Rheo-NMRによるタンパク質のアミロイド線維化過程のリアルタイム計測
菅瀬謙治
第21回日本蛋白質科学会年会, Jun. 2021, Invited

高感度Rheo-NMRによる剪断流中のタンパク質の動的構造解析
菅瀬謙治
20-1 NMR研究会, 20 Dec. 2020, Invited

In situ Monitoring of Amyloid Fibril Formation Processes Using Rheo-NMR
Sugase K; Morimoto D; Walinda E; Iwakawa N; Yamamoto A; Scheler U
8th Asia-Pacific NMR Symposium 2019, 04 Jul. 2019, Invited

In situ monitoring of amyloid fibril formation processes using Rheo-NMR
Kenji Sugase
RIKEN Symposium Recent Progress in Protein Conformation and Aggregation, 24 Jan. 2019, Invited

In situ Monitoring of Protein Fibrillation Using High-Sensitivity Rheo-NMR
SUGASE Kenji
日本表面真空学会 2018年9月研究例会, 28 Sep. 2018, Invited

High-Sensitivity Rheo-NMR Spectroscopy for Protein Studies
Kenji Sugase
JGP-Chem Kyoto-Bordeaux Faculty & Student Research Workshop on Chiral Nanostructures for Photonic Applications, 26 Oct. 2017, Invited

天然変性タンパク質の動的構造研究の進展
菅瀬謙治
第89回日本生化学会大会, 27 Sep. 2016, Invited

Elucidation of potential sites for antibody engineering by fluctuation editing
Kenji Sugase
The 5th International Symposium on Drug Discovery and Design by NMR, 30 Aug. 2016, Invited

天然変性タンパク質の多様な標的分子結合機構
菅瀬謙治
第16回日本蛋白質科学会年会, 09 Jun. 2016, Invited

NMRによる生体高分子の動的構造解析
菅瀬謙治
新学術領域「柔らかな分子系」ワークショップ『ダイナミクス観測からタンパク質の「柔らかさ」を観る』, 01 Mar. 2016, Invited

NMRによる天然変性タンパク質の動的構造の先端研究
菅瀬謙治
日本分光学会NMR分光部会集中講義, 30 Sep. 2015, Invited

Heme oxygenase-2 activity regulated by the C-terminal intrinsically disordered region
Kenji Sugase
The 11th Japan-Korea Bilateral Symposium on Biological NMR & International NMR Symposium on Pharmaceutical NMR, 19 Dec. 2014, Invited

ヒト主要組織適合複合体の動的なペプチド認識と構造維持機構
菅瀬謙治
日本生物物理学会東北支部会2014, 05 Sep. 2014, Invited

Structural dynamics of Sox2 upon binding to DNA
Kenji Sugase
ICMRBS XXVI, 25 Aug. 2014, Invited

Dynamics of nuclear proteins analyzed by relaxation NMR spectroscopy
Kenji Sugase
The 4th Asia Pacific Protein Association Conference, 18 May 2014, Invited

HLA-HIV由来抗原ペプチド複合体-水のダイナミクスと抗原提示能
菅瀬謙治
関西創薬化学コミュニティ, 07 Mar. 2014, Invited

Mechanism of the distal regulation of substrate binding through intrinsic fluctuation in an enzyme
Kenji Sugase
The 10th Korea-Japan Bilateral Symposium on Biological NMR, 04 Jul. 2013, Invited

揺らぎが制御するヘム分解酵素反応の遠位効果
菅瀬謙治
第14回若手NMR研究会, 30 Jun. 2013, Invited

Regulation of substrate-binding by a hidden distal effect via intrinsic fluctuation
Kenji Sugase
Academia Sinica Seminor, 30 Jan. 2013, Invited

R2 dispersion spectroscopy
Kenji Sugase
2013 Taiwan magnetic resonance society annual meeting, 29 Jan. 2013, Invited

Mechanism of coupled folding and binding of an intrinsically disordered protein
Kenji Sugase
2013 Taiwan magnetic resonance society annual meeting, 29 Jan. 2013, Invited

Regulation of substrate-binding by a hidden distal effect via intrinsic fluctuation
Kenji Sugase
4-th France-Japan Joint Seminar, 07 Jan. 2013, Invited

Relaxation NMR for protein dynamics and interaction studies
菅瀬謙治
Pharmaceutical NMR lecture series in Osaka, 25 Oct. 2012, Invited

動的構造解析による酵素ヘムオキシゲナーゼのヘム認識機構の解明
菅瀬謙治
第45回構造生物応用研究会, 16 Jun. 2012, Invited

揺らぎから機能をどのように見るか
菅瀬謙治
よこはまNMR構造生物学研究会第43回ワークショップ：計算科学とNMRの融合に向けて, 06 Feb. 2012, Invited

天然変性タンパク質の共役した折り畳みと結合のメカニズム
菅瀬謙治
神戸大学先端融合科学シンポジウム, 30 Jan. 2012, Invited

蛋白質の構造変化を捉えるNMR分光法
菅瀬謙治
京都大学分子工学コロキウム, 20 Dec. 2011, Invited

動的構造解析による酵素ヘムオキシゲナーゼの機能発現メカニズムの解明
菅瀬謙治
第17回創薬情報研究会, 13 Sep. 2011, Invited

ヘム分解酵素の動的構造解析-蛋白質工学への応用をめざして
菅瀬謙治
よこはまNMR構造生物学研究会第41回ワークショップ「最先端NMR技術で切り拓く構造生物学の未来」, 02 Mar. 2011, Invited

核内蛋白質の構造変化と機能
菅瀬謙治
首都大学東京コロキウム, 26 Jan. 2011, Invited

NMRによる蛋白質の動的構造解析－核内蛋白質と酵素の標的分子認識機構－
菅瀬謙治
大阪大学バイオ関連多目的研究施設セミナー, 11 Nov. 2010, Invited

Fluctuation and function of nuclear proteins
菅瀬謙治; 原田英里砂
第48回日本生物物理学会年会, 20 Sep. 2010, Invited

ＮＭＲによるヘムオキシゲナーゼのヘム認識機構の解明
原田英里砂; 杉島正一; 野口正人; 福山恵一; 菅瀬謙治
大阪大学蛋白質研究所セミナー, 30 Jul. 2010, Invited

NMRによる天然変性タンパク質の動的構造解析
菅瀬謙治
第10回日本蛋白質科学会年会, 18 Jun. 2010, Invited

動きの面から見た核内タンパク質の機能
菅瀬謙治
ターゲットタンパク研究プログラム第3回研究交流会, 04 Mar. 2010, Invited

天然変性状態と機能の相関
菅瀬謙治
第32回日本分子生物学会年会, 11 Dec. 2009, Invited

Elucidating protein-protein interactions using relaxation dispersion spectroscopy
菅瀬謙治
大阪大学産業科学研究所セミナー, 16 Oct. 2009, Invited

天然変性タンパク質の構造と機能の最前線
菅瀬謙治
第49回生命科学夏の学校, 30 Aug. 2009, Invited

天然変性タンパク質の構造変化と機能
菅瀬謙治
北海道大学セミナー, 01 Jun. 2009, Invited

Elucidating protein-protein interactions using relaxation spectroscopy on high-field NMR
Kenji Sugase
IMS Nanoforum “New Vistas of Molecular Sciences by Ultra-high Field NMR Spectroscopy”, 22 Jan. 2009, Invited

Relaxation Dispersion法を用いた機能相関
菅瀬謙治
BMB2008, 11 Dec. 2008, Invited

NMRで分かる分子間相互作用のダイナミクス
菅瀬謙治
立命館大学理工学研究所シンポジウムタンパク質NMR研究の最前線, 29 Nov. 2008, Invited

緩和速度解析による“見えない相互作用”の検出
菅瀬謙治
理研NMR講演会, 06 Oct. 2008, Invited

NMRで分かるタンパク質のダイナミクス
菅瀬謙治
第9回若手NMR研究会, 15 Sep. 2008, Invited

NMRで分かる分子間相互作用のダイナミクス
菅瀬謙治
横浜市立大学セミナー, 05 Sep. 2008, Invited

天然変性蛋白質と遭遇複合体
菅瀬謙治; H. Jane Dyson; Peter E. Wright
第8回日本蛋白質科学会年会, 10 Jun. 2008, Invited

NMRによる構造変化の研究
菅瀬謙治
大阪大学蛋白質研究所セミナー, 03 Mar. 2008, Invited

Relaxation dispersion NMRによる準安定構造の検出
菅瀬謙治; H. Jane Dyson; Peter E. Wright
日本生物物理学会第45回年会, 21 Dec. 2007, Invited

天然変性蛋白の連結した結合と折り畳みのメカニズム
菅瀬謙治
GCOE・P&P先端技術講習会, 07 Nov. 2007, Invited

Protein-protein interaction characterized by NMR relaxation dispersion spectroscopy
Kenji Sugase
International Symposium on Drug Discovery and Design by NMR, 22 Oct. 2007, Invited

NMR Relaxation dispersion法により描写される蛋白質－蛋白質相互作用の中間体
菅瀬謙治
日本薬学会東海支部特別講演会, 19 Sep. 2007, Invited

結合と連動した蛋白質フォールディングの熱力学と動力学
菅瀬謙治; H. Jane Dyson; Peter E. Wright
大阪大学蛋白質研究所セミナー, 29 Sep. 2006, Invited

Books and Other Publications

Elucidating Functional Dynamics by R1ρ and R2 Relaxation Dispersion NMR Spectroscopy. In: Experimental Approaches of NMR Spectroscopy
Erik Walinda; Kenji Sugase, Contributor, 263-290
Springer Nature, Mar. 2025

タンパク質の構造解析手法と In silicoスクリーニングへの応用事例～AlphaFold、In silico創薬、NMR、X線、クライオ電子顕微鏡～
森本大智; 菅瀬謙治, Contributor, 第3節タンパク質を多角的に捉えるNMR測定法
技術情報協会, Jul. 2023

NMRによる有機材料分析とその試料前処理、データ解釈
森本大智; 菅瀬謙治, Joint work, 第2節 Rheo-NMRによる生体高分子の動的構造解析
技術情報協会, Sep. 2021
Find Kyoto University's collection of books :

Experimental Approaches of NMR Spectroscopy
Walinda E; Sugase K, Joint work, chapter 7: Elucidating functional dynamics by R1ρ and R2 relaxation dispersion NMR spectroscopy.
Springer, 2018, Not refereed
Find Kyoto University's collection of books :

揺らぎ・ダイナミクスと生体機能物理化学的視点から見た生体分子
菅瀬謙治, Joint work, 5章 NMR
化学同人, Sep. 2013, Not refereed

Handbook of Oxytocin Research: Synthesis, Storage and Release, Actions and Drug Forms
Kawada T; Sekiguchi T; Sugase K; Kanda A; Satake H, Joint work, 59-85
Nova Science Publishers, 2009, Not refereed

Water and Biomolecules: Physical Chemistry of Life Phenomena (Biological and Medical Physics, Biomedical Engineering)
Wright PE; Felitsky DJ; Sugase K; Dyson HJ, Joint work, 1-11
Springer-Verlag, 2009, Not refereed

Industrial Property Rights

特願2025-206905, 流れに対するタンパク質の凝集速度算出方法及び安定性評価方法
菅瀬謙治; 古川亜矢子; 宗正智; 金書林

特願2024-128950, 剪断流を発生させる器具
菅瀬謙治; 山本昭彦; 宗正智; 保科好秀

特願2023-170462, 剪断流を発生させる器具
菅瀬謙治; 森本大智; 山本昭彦; 保科好秀

特許第7255801号, 特開2020-204481, 特願2019-111013, 剪断流を発生させる器具
菅瀬謙治; 森本大智; 保科好秀

Awards

09 Apr. 2025
Kyoto University
Kyoto University SPIRIT2 Award, Japan, Others
Rheo-NMRとソフトマター物理の融合による分子流動ダイナミクスの革新的解析

Media Coverage

ATPがタンパク質凝集を抑制
科学新聞, 3面, 20 Aug. 2021, Paper

たんぱく質アミロイド線維化 ATP、結合抑える
日刊工業新聞, 17面, 13 Aug. 2021, Paper

高分解能で過程観察アミロイド線維化発症の仕組み解明へ
日刊工業新聞2, 20 Jul. 2021, Paper

タンパク質同士の結合観察
日経産業新聞, 11面, 24 May 2007, Paper

External funds: Kakenhi

NMR study on local structural fluctuations of double-stranded DNA
Grant-in-Aid for Transformative Research Areas (A)
Transformative Research Areas, Section (III)
Kyoto University
菅瀬謙治
From 10 Sep. 2021, To 31 Mar. 2023, Discontinued
NMR;二本鎖DNA;動的構造;エピゲノム修飾;溶液環境
https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-21H05746/

Elucidation of the mechanism of amyloid fibrillation of alpha-synuclein starting from liquid-liquid phase separation
Grant-in-Aid for Scientific Research (B)
Basic Section 43040:Biophysics-related
Kyoto University
Kenji Sugase
From 01 Apr. 2021, To 31 Mar. 2024, Project Closed
液-液相分離;アミロイド線維化;αシヌクレイン;剪断流;Rheo-NMR;アミロイド線維
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H02442/

Development of a new methodology for determining dissociation constants of proteins and drugs in cells
Grant-in-Aid for Challenging Research (Exploratory)
Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
Kyoto University
Kenji Sugase
From 30 Jul. 2020, To 31 Mar. 2022, Project Closed
タンパク質－薬剤相互作用;難水溶性薬剤;解離定数;in-cell NMR;19F-NMR;化学交換;タンパク質‐薬剤相互作用;細胞内環境;NMR
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K21391/

Velocity imaging of turbulence flow using Rheo-NMR
Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
Medium-sized Section 13:Condensed matter physics and related fields
Kyoto University
Kenji Sugase
From 09 Oct. 2018, To 31 Mar. 2022, Project Closed
Rheo-NMR;速度イメージング;off-center Couette cell;乱流;タンパク質凝集;線維化
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18KK0075/

Analysis of the effects of crowded environment and shear force to proteins using Rheo-NMR
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Kyoto University
菅瀬謙治
From 01 Apr. 2018, To 31 Mar. 2020, Project Closed
細胞内夾雑環境;タンパク質;安定化/不安定化;アミロイド線維化;ATP;NMR;Rheo-NMR;夾雑環境;剪断力;安定性;細胞抽出液
https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-18H04551/

Elucidation of the mechanism of amyloid fibrillation using Rheo-NMR and development of a new Rheo-NMR instrument
Grant-in-Aid for Challenging Research (Exploratory)
Kyoto University
Kenji Sugase
From 30 Jun. 2017, To 31 Mar. 2020, Project Closed
Rheo-NMR;タンパク質;アミロイド線維;NMR;レオロジー;蛋白質;生物物理;流体
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K19196/

Activation mechanism of ubiquitin ligase parkin by PINK1 and phosphorylated ubiquitin
Grant-in-Aid for Scientific Research (B)
Kyoto University
Kenji Sugase
From 01 Apr. 2015, To 31 Mar. 2019, Project Closed
parkin;リフォールディング;大量発現系;NMR;タンパク質;分子認識;Parkin;ユビキチン;動的構造;PINK1;リン酸化ユビキチン;蛋白質;生物物理;高分子構造・物性
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15H04342/

Ultra-fast NMR measurement to analyze dynamics of Sox2 in cells
Grant-in-Aid for Challenging Exploratory Research
Suntory Foundation for Life Sciences
Kenji SUGASE
From 01 Apr. 2013, To 31 Mar. 2015, Project Closed
in-cell NMR;タンパク質;揺らぎ;高速測定;高速NMR法
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25650056/

Elucidating a DNA recognition mechanism of a transcriptional factor protein by characterizing dynamic processes of specific/non-specific DNA-bound complexes
Grant-in-Aid for Young Scientists (B)
Suntory Foundation for Life Sciences
ERISA HARADA
From 01 Apr. 2012, To 31 Mar. 2014, Project Closed
NMR;蛋白質構造
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24770106/

Elucidation of dynamics and function of an iPS cell reprogramming factor Sox2 from the view point of an intrinsically disordered protein
Grant-in-Aid for Young Scientists (B)
Suntory Institute for Bioorganic Research
Kenji SUGASE
From 01 Apr. 2009, To 31 Mar. 2011, Project Closed
分子認識及び相互作用;分子認識および相互作用
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770126/

Mechanism analysis of sulfobetaines' activity to prevent protein aggregation and application of sulfobetaines to unstable proteins
Grant-in-Aid for Scientific Research (C)
Gunma University
Kaori WAKAMATSU
From 01 Apr. 2009, To 31 Mar. 2012, Project Closed
タンパク質;凝集防止;安定化;スルホベタイン;メカニズム;運動性;オスモライト;分子サイズ;溶解度;凝集;変性;抗体;精製;収率;効率化;構造多型;NMR;NDSB;立体構造解析
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21570108/

Iron acquisition mechanism of graminaceous plants by mugineic acids
Grant-in-Aid for Scientific Research (B)
Suntory Institute for Bioorganic Research
Yoshiko MURATA
From 01 Apr. 2009, To 31 Mar. 2012, Project Closed
ムギネ酸;鉄錯体;イネ科植物;トランスポーター;ファイトシデロフォア;ムギネ酸鉄錯体トランスポーター;基質選択性;イネ科食物;オオムギ;光親和標識体;鉄輸送;標識体
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21310148/

Characterization of structural dynamics of intrinsically disordered proteins
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Biological Sciences
Yokohama City University
Satoko AKASHI
From 23 Jul. 2009, To 31 Mar. 2014, Project Closed
蛋白質;生体分子;NMR;質量分析;天然変性;ＮＭＲ;蛋白;生子
https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-21113003/

NMR緩和分散法によるiPS細胞誘導因子Oct3/4の揺らぎの検出
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Science and Engineering
Suntory Institute for Bioorganic Research
菅瀬謙治
From 01 Apr. 2009, To 31 Mar. 2011, Project Closed
揺らぎの検出;蛋白質;NMR
https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-21107531/

膜との相互作用によるグレリンの生理活性の調節機構
Grant-in-Aid for Young Scientists (B)
Suntory Institute for Bioorganic Research
菅瀬謙治
From 01 Apr. 2003, To 31 Mar. 2005, Project Closed
グレリン;リン脂質膜;マイクロカロリメトリー
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15710169/

Elucidation of the mechanism of protein aggregation in flow by using Rheo-NMR and Rheo-MD
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Complex systems
Kyoto University
菅瀬謙治
From 01 Apr. 2022, To 31 Mar. 2023, Discontinued
剪断流;会合;Rheo-NMR;Rheo-MD;αシヌクレイン
https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-22H04842/

Atomic-level analysis of proteins responding to dynamic solution environments using NMR
Grant-in-Aid for Transformative Research Areas (B)
Transformative Research Areas, Section (III)
Kyoto University
Kenji Sugase
From 20 May 2022, To 31 Mar. 2025, Project Closed
ATP;液滴;アミロイド線維;Rheo-NMR;電場NMR;eMD;非膜オルガネラ;in-cell NMR;膜なしオルガネラ
https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-22H05088/

Advancement of Dynamic Solution Science
Grant-in-Aid for Transformative Research Areas (B)
Transformative Research Areas, Section (III)
Kyoto University
Naotaka Sekiyama
From 20 May 2022, To 31 Mar. 2025, Project Closed
天然変性タンパク質;液液相分離;アミロイド線維;非膜型オルガネラ;動的溶液環境;液-液相分離;アミロイド線維化
https://kaken.nii.ac.jp/grant/KAKENHI-ORGANIZER-22H05087/

Development of analytical platform for proteins in electric field and its application
Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
Kyoto University
菅瀬謙治
From 07 Oct. 2022, To 31 Mar. 2026, Granted
電場;NMR;タンパク質;電気泳動;凝集;分子動力学計算
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KK0098/

Investigation of molecular aggregation control mechanism by solution environment and creation of molecular design tools
Grant-in-Aid for Scientific Research (B)
Basic Section 32010:Fundamental physical chemistry-related
Nagoya University
吉田紀生
From 01 Apr. 2024, To 31 Mar. 2028, Granted
3D-RISM;分子凝集;相分離;シミュレーション;酸化還元;超分子ゲル;分子シミュレーション;モンテカルロ法
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K01434/

Rheo-NMR and paramagnetic relaxation-based structural determination of alpha-synuclein oligomers
Grant-in-Aid for Scientific Research (B)
Basic Section 43040:Biophysics-related
Kyoto University
菅瀬謙治
From 01 Apr. 2025, To 31 Mar. 2028, Granted
αシヌクレイン;オリゴマー;Rheo-NMR;常磁性緩和法
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25K02240/

External funds: others

緑茶成分EGCGと没食子酸の混合物によるαシヌクレイン線維化抑制機構の解明とパーキンソン病予防
一般財団法人東洋水産財団, 2026年度学術奨励研究
From 01 Jul. 2026, To 31 Mar. 2027

流動ストレス下におけるバイオ医薬品安定性の定量評価解析プラットフォーム構築
国立研究開発法人科学技術振興機構, KSAC-GAP ファンド
From 01 Apr. 2026, To 31 Mar. 2027

抗体医薬品の流れ安定性を迅速かつ定量的に評価するシステム開発補助事業 (2025年度分)
公益財団法人JKA, 自転車等機械振興事業
From 01 Apr. 2025, To 31 Mar. 2026
研究代表者

流れに対するバイオ医薬品の安定性を定量評価できる装置開発　補助事業 (2024年度分)
公益財団法人JKA, 自転車等機械振興事業
From 01 Apr. 2024, To 31 Mar. 2025
研究代表者

電場中の生体分子の挙動を原子レベルで解析できる高感度・高分解能電場NMR装置の開発 (2023年度分)
公益財団法人JKA, 自転車等機械振興事業
From 01 Apr. 2023, To 31 Mar. 2024
研究代表者

Development of molecular tools to analyze and control the liquid-liquid phase separation of proteins conjugated with redox states
豊田理研, 豊田理研スカラー共同研究
From 01 Apr. 2022, To 31 Mar. 2023
菅瀬謙治

操作性良く高感度測定ができる新型Rheo-NMR装置の開発　 (2022年度分)
公益財団法人JKA, 自転車等機械振興事業
From 01 Apr. 2022, To 31 Mar. 2023
研究代表者

緑茶カテキンによるアミロイド線維化抑制機構の解明
一般財団法人東洋水産財団, 学術研究助成
From 01 Jun. 2022, To 31 Mar. 2023
菅瀬謙治
代表

新型コロナウイルス感染の重篤化に繋がるvWFが流れにより構造変化する機構の解明
公益財団法人小柳財団, 研究助成
From 01 Apr. 2022, To 31 Mar. 2023
菅瀬謙治
代表

新型コロナ感染の重症化を引き起こす vWF の構造変化機構の解明
東京化成化学振興財団, 研究助成
From 01 Apr. 2022, To 31 Mar. 2023
菅瀬謙治
代表

生命科学におけるATP-Mg会合体の構造と機能の解明
京都大学教育研究振興財団, 研究活動推進支援
From 01 May 2020, To 31 Mar. 2021
菅瀬謙治
代表

光照射化における励起状態解析に基づく機能性分子の物性評価
公益財団法人豊田理化学研究所, 豊田理研スカラー共同研究(Phase 2)
From 01 Apr. 2020, To 31 Mar. 2021
菅瀬謙治
代表

光照射化における励起状態解析に基づく機能性分子の物性評価
公益財団法人豊田理化学研究所, 豊田理研スカラー共同研究(Phase 1)
From 01 Nov. 2019, To 31 Mar. 2020
菅瀬謙治
代表

剪断力により誘起される相分離の機構解明
公益財団法人豊田理化学研究所, 豊田理研スカラー共同研究(Phase 2)
From 01 Apr. 2019, To 31 Mar. 2020
菅瀬謙治
代表

剪断力により誘起される相分離の機構解明
公益財団法人豊田理化学研究所, 豊田理研スカラー共同研究 (Phase 1)
From 01 Nov. 2018, To 31 Mar. 2019
菅瀬謙治
代表

世界最高感度Rheo-NMR装置の開発
公益財団法人豊田理化学研究所, 豊田理研スカラー
From 01 Apr. 2018, To 31 Mar. 2018
菅瀬謙治
代表

緑茶カテキンによるパーキンソン病予防効果の科学的立証
一般財団法人生産開発科学研究所, 生研学術奨励資金
From 01 Feb. 2018, To 31 Jan. 2019
菅瀬謙治

in-cell NMRによる細胞中のライソゾーム病関連酵素の構造解析
日本応用酵素協会, 酵素研究助成
From 01 Apr. 2017, To 31 Mar. 2018
菅瀬謙治
代表

Rheo-NMR法によるアミロイド線維形成メカニズムの解明
自然科学研究機構, 融合発展促進研究プロジェクト
From 01 Oct. 2016, To 31 Mar. 2018
菅瀬謙治
代表

操作性良く高感度測定ができる新型Rheo-NMR装置の開発補助事業 (2021年度分)
公益財団法人JKA, 自転車等機械振興事業
From 01 Apr. 2021, To 31 Mar. 2022
代表

Mechanism of the coupled folding and binding of an intrinsically disordered protein
The Skaggs Scholarship
From 01 Sep. 2005, To 31 Aug. 2006
Kenji Sugase

Last Updated :2026/06/26

Education

Teaching subject(s)

From 01 Apr. 2026, To 31 Mar. 2027
Advanced Applied Biochemistry
CA29, Fall, Graduate School of Agriculture, 1

From 01 Apr. 2026, To 31 Mar. 2027
Biomacromolecular Chemistry
CA21, Spring, Graduate School of Agriculture, 1

From 01 Apr. 2026, To 31 Mar. 2027
Applied Life Sciences III
CA03, Spring, Graduate School of Agriculture, 1

From 01 Apr. 2026, To 31 Mar. 2027
Macromolecular Bioscience Seminar
CF02, Fall, Graduate School of Agriculture, 1.5

From 01 Apr. 2026, To 31 Mar. 2027
Macromolecular Bioscience Seminar
CF02, Spring, Graduate School of Agriculture, 1.5

From 01 Apr. 2026, To 31 Mar. 2027
Experimental Course of Macromolecular Bioscience
CG02, Spring, Graduate School of Agriculture, 3

From 01 Apr. 2026, To 31 Mar. 2027
Experimental Course of Macromolecular Bioscience
CG02, Fall, Graduate School of Agriculture, 3

From 01 Apr. 2026, To 31 Mar. 2027
Scientific English
5664, Spring, Faculty of Agriculture, 2

From 01 Apr. 2026, To 31 Mar. 2027
Structure and Function of Biomacromolecules
5635, Fall, Faculty of Agriculture, 2

From 01 Apr. 2026, To 31 Mar. 2027
General Biomacromolecular Chemistry
5633, Spring, Faculty of Agriculture, 2

From 01 Apr. 2026, To 31 Mar. 2027
Outline of Applied Life Sciences
5801, Spring, Faculty of Agriculture, 2

From 01 Apr. 2026, To 31 Mar. 2027
Analytical Chemistry
5622, Spring, Faculty of Agriculture, 2

From 01 Apr. 2026, To 31 Mar. 2027
Introduction to Applied Life Science I
5601, Spring, Faculty of Agriculture, 2

From 01 Apr. 2025, To 31 Mar. 2026
Advanced Applied Biochemistry
CA29, Fall, Graduate School of Agriculture, 1

From 01 Apr. 2025, To 31 Mar. 2026
Biomacromolecular Chemistry
CA21, Spring, Graduate School of Agriculture, 1

From 01 Apr. 2025, To 31 Mar. 2026
Applied Life Sciences III
CA03, Spring, Graduate School of Agriculture, 1

From 01 Apr. 2025, To 31 Mar. 2026
Macromolecular Bioscience Seminar
CF02, Fall, Graduate School of Agriculture, 1.5

From 01 Apr. 2025, To 31 Mar. 2026
Macromolecular Bioscience Seminar
CF02, Spring, Graduate School of Agriculture, 1.5

From 01 Apr. 2025, To 31 Mar. 2026
Experimental Course of Macromolecular Bioscience
CG02, Spring, Graduate School of Agriculture, 3

From 01 Apr. 2025, To 31 Mar. 2026
Experimental Course of Macromolecular Bioscience
CG02, Fall, Graduate School of Agriculture, 3

From 01 Apr. 2025, To 31 Mar. 2026
Structure and Function of Biomacromolecules
5635, Fall, Faculty of Agriculture, 2

From 01 Apr. 2025, To 31 Mar. 2026
General Biomacromolecular Chemistry
5633, Spring, Faculty of Agriculture, 2

From 01 Apr. 2025, To 31 Mar. 2026
Analytical Chemistry
5622, Spring, Faculty of Agriculture, 2

From 01 Apr. 2025, To 31 Mar. 2026
Outline of Applied Life Sciences
5801, Spring, Faculty of Agriculture, 2

From 01 Apr. 2025, To 31 Mar. 2026
Introduction to Applied Life Science I
5601, Spring, Faculty of Agriculture, 2

From 01 Apr. 2024, To 31 Mar. 2025
Applied Life Sciences III
CA03, Spring, Graduate School of Agriculture, 1

From 01 Apr. 2024, To 31 Mar. 2025
Biomacromolecular Chemistry
CA21, Spring, Graduate School of Agriculture, 1

From 01 Apr. 2024, To 31 Mar. 2025
Advanced Applied Biochemistry
CA29, Fall, Graduate School of Agriculture, 1

From 01 Apr. 2024, To 31 Mar. 2025
Macromolecular Bioscience Seminar
CF02, Spring, Graduate School of Agriculture, 1.5

From 01 Apr. 2024, To 31 Mar. 2025
Macromolecular Bioscience Seminar
CF02, Fall, Graduate School of Agriculture, 1.5

From 01 Apr. 2024, To 31 Mar. 2025
Experimental Course of Macromolecular Bioscience
CG02, Spring, Graduate School of Agriculture, 3

From 01 Apr. 2024, To 31 Mar. 2025
Experimental Course of Macromolecular Bioscience
CG02, Fall, Graduate School of Agriculture, 3

From 01 Apr. 2024, To 31 Mar. 2025
General Biomacromolecular Chemistry
5633, Spring, Faculty of Agriculture, 2

From 01 Apr. 2024, To 31 Mar. 2025
Analytical Chemistry
5622, Spring, Faculty of Agriculture, 2

From 01 Apr. 2024, To 31 Mar. 2025
Introduction to Applied Life Science I
5601, Spring, Faculty of Agriculture, 2

From 01 Apr. 2024, To 31 Mar. 2025
Outline of Applied Life Sciences
5801, Spring, Faculty of Agriculture, 2

From 01 Apr. 2024, To 31 Mar. 2025
Structure and Function of Biomacromolecules
5635, Fall, Faculty of Agriculture, 2

From 01 Apr. 2023, To 31 Mar. 2024
Biomacromolecular Chemistry
CA21, Spring, Graduate School of Agriculture, 1

From 01 Apr. 2023, To 31 Mar. 2024
Applied Life Sciences III
CA03, Spring, Graduate School of Agriculture, 1

From 01 Apr. 2023, To 31 Mar. 2024
Advanced Applied Biochemistry
CA29, Fall, Graduate School of Agriculture, 1

From 01 Apr. 2023, To 31 Mar. 2024
Macromolecular Bioscience Seminar2
CB20, Year-long, Graduate School of Agriculture, 3

From 01 Apr. 2023, To 31 Mar. 2024
Macromolecular Bioscience Seminar1
CB19, Year-long, Graduate School of Agriculture, 3

From 01 Apr. 2023, To 31 Mar. 2024
Laboratory Course in Biochemistry
5669, Year-long, Faculty of Agriculture, 3

From 01 Apr. 2023, To 31 Mar. 2024
Experimental Course of Macromolecular Bioscience2
CC20, Year-long, Graduate School of Agriculture, 6

From 01 Apr. 2023, To 31 Mar. 2024
Experimental Course of Macromolecular Bioscience1
CC19, Year-long, Graduate School of Agriculture, 6

From 01 Apr. 2023, To 31 Mar. 2024
Structure and Function of Biomacromolecules
5635, Fall, Faculty of Agriculture, 2

From 01 Apr. 2023, To 31 Mar. 2024
General Biomacromolecular Chemistry
5633, Spring, Faculty of Agriculture, 2

From 01 Apr. 2023, To 31 Mar. 2024
Analytical Chemistry
5622, Spring, Faculty of Agriculture, 2

From 01 Apr. 2023, To 31 Mar. 2024
Introduction to Applied Life Science I
5601, Spring, Faculty of Agriculture, 2

From 01 Apr. 2022, To 31 Mar. 2023
Advanced Applied Biochemistry
CA29, Fall, Graduate School of Agriculture, 1

From 01 Apr. 2022, To 31 Mar. 2023
Physical Chemistry: Fundamentals and Exercises
7102, Spring, Faculty of Engineering, 2

From 01 Apr. 2022, To 31 Mar. 2023
Experimental Course of Macromolecular Bioscience1
CC19, Year-long, Graduate School of Agriculture, 6

From 01 Apr. 2022, To 31 Mar. 2023
Macromolecular Bioscience Seminar1
CB19, Year-long, Graduate School of Agriculture, 3

From 01 Apr. 2022, To 31 Mar. 2023
Biomacromolecular Chemistry
CA21, Spring, Graduate School of Agriculture, 1

From 01 Apr. 2022, To 31 Mar. 2023
Applied Life Sciences III
CA03, Spring, Graduate School of Agriculture, 1

From 01 Apr. 2022, To 31 Mar. 2023
Macromolecular Bioscience Seminar2
CB20, Year-long, Graduate School of Agriculture, 3

From 01 Apr. 2022, To 31 Mar. 2023
Experimental Course of Macromolecular Bioscience2
CC20, Year-long, Graduate School of Agriculture, 6

From 01 Apr. 2022, To 31 Mar. 2023
Introduction to Applied Life Science I
5601, Spring, Faculty of Agriculture, 2

From Apr. 2015, To Mar. 2016
Physical Chemistry III (Fundamental Chemistry)
Fall, 工学部

From Apr. 2015, To Mar. 2016
Physical Chemistry: Fundamentals and Exercises
Spring, 工学部

From Apr. 2016, To Mar. 2017
Physical Chemistry III (Fundamental Chemistry)
Fall, 工学部

From Apr. 2016, To Mar. 2017
Physical Chemistry: Fundamentals and Exercises
Spring, 工学部

From Apr. 2016, To Mar. 2017
Biomolecular Function Chemistry
Spring, 工学研究科

From Apr. 2017, To Mar. 2018
Safty in Chemistry Laboratory
Spring, 工学部

From Apr. 2017, To Mar. 2018
Physical Chemistry III (Fundamental Chemistry)
Fall, 工学部

From Apr. 2017, To Mar. 2018
Physical Chemistry: Fundamentals and Exercises
Spring, 工学部

From Apr. 2018, To Mar. 2019
Safty in Chemistry Laboratory
Spring, 工学部

From Apr. 2018, To Mar. 2019
Physical Chemistry III (Fundamental Chemistry)
Fall, 工学部

From Apr. 2018, To Mar. 2019
Physical Chemistry: Fundamentals and Exercises
Spring, 工学部

From Apr. 2018, To Mar. 2019
Biomolecular Function Chemistry
Spring, 工学研究科

From Apr. 2019, To Mar. 2020
Safty in Chemistry Laboratory
Spring, 工学部

From Apr. 2019, To Mar. 2020
Physical Chemistry III (Fundamental Chemistry)
Fall, 工学部

From Apr. 2019, To Mar. 2020
Physical Chemistry: Fundamentals and Exercises
Spring, 工学部

From Apr. 2020, To Mar. 2021
Safty in Chemistry Laboratory
Spring, 工学部

From Apr. 2020, To Mar. 2021
Physical Chemistry: Fundamentals and Exercises
Spring, 工学部

From Apr. 2020, To Mar. 2021
Physical Chemistry III (Fundamental Chemistry)
Fall, 工学部

From Apr. 2020, To Mar. 2021
Biomolecular Function Chemistry
Spring, 工学研究科

From Apr. 2021, To Mar. 2022
Molecular Spectroscopy
Fall, 工学研究科

From Apr. 2021, To Mar. 2022
Safty in Chemistry Laboratory
Spring, 工学部

From Apr. 2021, To Mar. 2022
Physical Chemistry III (Advanced Chemistry)
Fall, 工学部

From Apr. 2021, To Mar. 2022
Physical Chemistry: Fundamentals and Exercises
Spring, 工学部

Participation in PhD Defense

Protein Structural Dynamics under Physical and Chemical Perturbations
SHUTO YUSUKE, Graduate School of Agriculture, Chief Examiner
23 Mar. 2026

Molecular mechanisms of vacuolar dynamics and lipid metabolism in yeast autophagy
NAKATSUJI TAKUMI, Graduate School of Agriculture, Sub-chief Examiner
23 Mar. 2026

Development of a convenient microbial-growth evaluation system based on microscopic image analysis
TAMIYA HISATO, Graduate School of Agriculture, Sub-chief Examiner
23 Mar. 2026

Development of physically stabilized bilayer lipid membranes using a track-etched membrane and its application to elucidate ion transport phenomena across bilayer lipid membranes in the presence of various ionophores
CHUANG WEIPAI, Graduate School of Agriculture, Sub-chief Examiner
24 Sep. 2025

紅色進化系統藻類における光捕集タンパク質複合体の分子進化と機能
KUMAZAWA MINORU, Graduate School of Agriculture, Sub-chief Examiner
24 Mar. 2025

Reconstitution of ribosome self-replication
KOSAKA YUISHIN, Graduate School of Agriculture, Chief Examiner
24 Mar. 2025

Evaluation of the structure, dynamics, and thermodynamic propertiesof nucleic acids forming triplex structures in intracellular environments
SAKAMOTO TOMOKI, Graduate School of Energy Science, Sub-chief Examiner
24 Mar. 2025

膜結合型フルクトース脱水素酵素における直接電子移動型酵素電極反応の構造生物電気化学的考察とその応用
SUZUKI YOHEI, Graduate School of Agriculture, Sub-chief Examiner
24 Mar. 2025

化学物質の微生物による分解特性とその要因解析
TAKANO YOSHINARI, Graduate School of Agriculture, Sub-chief Examiner
24 Sep. 2024

Physiological and biochemical studies on microbial 2´-deoxyribonucleotide　biosynthesis and oxidative pyrimidine metabolism
DEGUCHI KENGO, Graduate School of Agriculture, Sub-chief Examiner
25 Mar. 2024

Insights into the structure and functionality of feruloyl esterases for the efficient utilization of lignocellulosic biomass
APISAN PHIENLUPHON, Graduate School of Energy Science, Sub-chief Examiner
25 Mar. 2024

Development of functional cellomics for comprehensive analysis of the relationship between neural networks and behavior in Caenorhabditis elegans
YAMAUCHI YUJI, Graduate School of Agriculture, Chief Examiner
23 Mar. 2023

Development of Direct Electron Transfer-Type Cascade System by Alcohol and Aldehyde Dehydrogenases
ADACHI TAIKI, Graduate School of Agriculture, Sub-chief Examiner
23 Mar. 2023

Student achievements: Awards

若手ポスター賞
李勇進, 第64回NMR討論会

MBSJ-EMBO Poster Award 2025
齋藤元伸, 第48回日本分子生物学会年会, 16 Dec. 2025

APNMR Poster Award
Kota Yoshioka, ISMAR-APNMR2021, 27 Aug. 2021

APNMR Poster Award
Takuya Hirakawa, ISMAR-APNMR, 27 Aug. 2021

Student achievements: Papers

Conformational Fluctuations and Induced Orientation of a Protein, Its Solvation Shell, and Bulk Water in Weak Non-Unfolding External Electric Fields
Yusuke Shuto, Erik Walinda, Daichi Morimoto, Kenji Sugase
16 Aug. 2023, First author, The Journal of Physical Chemistry B, 127, 34, 7417, 7430, Peer-reviewed, English, Scientific journal, False
https://doi.org/10.1021/acs.jpcb.3c01683

Backbone resonance assignments of the CPEB3 [101–200] and CPEB3 [294–410]
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase
29 Mar. 2025, First author, Second author, Biomolecular NMR Assignments, 19, 1, 109, 114, Peer-reviewed, English, Scientific journal, False
https://doi.org/10.1007/s12104-025-10226-5

Purification tags markedly affect self‐aggregation of CPEB3
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase
17 Jun. 2025, First author, Second author, FEBS Letters, 599, 19, 2779, 2789, Peer-reviewed, English, Scientific journal, False
https://doi.org/10.1002/1873-3468.70090

Moiety-specific mechanism of ATP's hydrotropic action on α-synuclein
Yusuke Shuto, Toshifumi Mori, Benjamin Kohn, Erik Walinda, Daichi Morimoto, Ulrich Scheler, Masatomo So, Ayako Furukawa, Norio Yoshida, Kenji Sugase
24 Apr. 2026, First author, Physical Chemistry Chemical Physics, 28, 18, 11221, 11233, Peer-reviewed, English, Scientific journal, True
https://doi.org/10.1039/d6cp00884d

Mechanically stable high-sensitivity Rheo-NMR spectroscopy for real-time monitoring of shear-induced phase transitions
Akihiko Yamamoto, Yusuke Shuto, Daichi Morimoto, Ulrich Scheler, Ayako Furukawa, Masatomo So, Kenji Sugase
07 Jun. 2026, Second author, Journal of Magnetic Resonance, 390, 108101, Peer-reviewed, Invited, English, Scientific journal, True
https://doi.org/10.2139/ssrn.6714336

1H, 13C, and 15N backbone resonance assignments of the A2 domain of human von Willebrand factor
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Daichi Morimoto, Kenji Sugase
23 Mar. 2026, First authoer, Biomolecular NMR Assignments, 20, 1, Peer-reviewed, English, Scientific journal, False
https://doi.org/10.1007/s12104-026-10263-8

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase
20 May 2026, First author, FEBS Letters, Peer-reviewed, English, Scientific journal, False
https://doi.org/10.1002/1873-3468.70366

Backbone resonance assignments of CPEB3 [1–120], CPEB3 [186–315], and CPEB3 [400–459]
Yujin Lee, Harunobu Saito, Masatomo So, Ayako Furukawa, Kenji Sugase
29 May 2025, First author, Second author, Biomolecular NMR Assignments, 20, 1, Peer-reviewed, English, Scientific journal, False
https://doi.org/10.1007/s12104-026-10269-2

Leucyl-tRNA Synthetase as a Molecular Target of Isobutanol-Mediated Growth Inhibition in Saccharomyces cerevisiae
Mano Hasegawa, Nodoka Oshimura, Kaho Hitomi, Ayako Furukawa, Kenji Sugase, Kouichi Kuroda
04 Jun. 2026, First author, Journal of Biological Chemistry, Peer-reviewed, English, Scientific journal, False
https://doi.org/10.1016/j.jbc.2026.113228

The Effect of Protein Tagging on Aggregation and Phase Separation
Harunobu Saito, Kenji Sugase
14 Jun. 2026, First author, Journal of Cellular Biochemistry, 127, 6, Peer-reviewed, Invited, English, Scientific journal, False
https://doi.org/10.1002/jcb.70096

Last Updated :2026/06/26

Administration

Faculty management (title, position)

From 01 Apr. 2026, To 31 Mar. 2027
防火・防災委員会　委員

From 01 Apr. 2026, To 31 Mar. 2027
評価委員会　委員

From 01 Apr. 2026, To 31 Mar. 2027
応用生命科学専攻長

From 01 Apr. 2023, To 31 Mar. 2027
将来構想検討委員会　委員

From 01 Apr. 2015, To 31 Mar. 2019
工学研究科・工学部図書委員会　委員

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Sugase, Kenji

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