Basic Information

Faculty

• 医学部

Academic Degree

• 修士（理学）(京都大学)
• 博士（理学）(京都大学)

Research History

• From 01 Apr. 2007

  Kyoto University, Graduate School of Medicine
• From 01 Apr. 2007

  Assistant Professor, Graduate School of Medicine, Kyoto University
• From 01 Jul. 2001

  Kyoto University, Graduate School of Medicine
• From 01 Jul. 2001

  Research Associate, Graduate School of Medicine, Kyoto University
• From 01 Apr. 2001

  日本学術振興会特別研究員(PD)
• From 01 Apr. 2001

  Research Fellow (PD), Japan Society for the Promotion of Science
• 現在に至る

Language of Instruction

• English

ID,URL

• J-Global ID

  200901066148464145

researchmap URL

• https://researchmap.jp/read0095902

Research

Research Topics, Overview of the research

• Research Topics

  Elucidation of novel molecular mechanisms that regulate cell division and cell proliferation

• Overview of the research

  IQGAP family proteins, comprised of IQGAP1, 2 and 3 in mammals, are known to be involved in diverse cellular functions. Among them IQGAP3 is the least studied to date, and our previous studies have clarified that this protein is important for cell proliferation and cell division. We have identified its functional effectors, but the molecular mechanisms with which IQGAP3 exerts its function are still poorly understood.
  It is very important to understand the structure of these molecules in order to clarify this point. Therefore, in our research we express and purify the recombinant proteins of IQGAP3 and its effector molecules, and analyze the structure of the complex, so that we can understand the mechanisms of action of IQGAP3 at the structural level.
  In this way, we want to elucidate the mechanisms of molecular functions of the signaling molecules at the three-dimensional structure level, based on the findings obtained from cell biological analyses. Recent studies have revealed that IQGAP family molecules have additional interesting functions. We have succeeded in identifying the effectors that directly bind to and control IQGAP in performing its function. To clarify the mechanism of this regulation, we are currently conducting cell biological and biochemical analyses of the mechanism of the formation and regulation of these complexes, as well as conducting structural analyses of these complexes by expressing and purifying recombinant proteins.
  Through these studies, we want to elucidate molecular functions of IQGAP proteins.

Research Interests

• 細胞生物学
• Cell Biology

Research Areas

• Life sciences, Cell biology

Papers

• Iqgap3-Ras axis drives stem cell proliferation in the stomach corpus during homoeostasis and repair

  Junichi Matsuo; Daisuke Douchi; Khine Myint; Naing Naing Mon; Akihiro Yamamura; Kazuyoshi Kohu; Dede Liana Heng; Sabirah Chen; Nur Astiana Mawan; Napat Nuttonmanit; Ying Li; Supriya Srivastava; Shamaine Wei Ting Ho; Nicole Yee Shin Lee; Hong Kai Lee; Makoto Adachi; Atsushi Tamura; Jinmiao Chen; Henry Yang; Ming Teh; Jimmy Bok-Yan So; Wei Peng Yong; Patrick Tan; Khay Guan Yeoh; Linda Shyue Huey Chuang; Sachiko Tsukita; Yoshiaki Ito

  Gut, Oct. 2021

  
• Involvement of IQGAP family proteins in the regulation of mammalian cell cytokinesis

  Makoto Adachi; Asami Kawasaki; Hisashi Nojima; Eisuke Nishida; Sachiko Tsukita

  GENES TO CELLS, Nov. 2014, Peer-reviewed

  
• Polarity-Dependent Distribution of Angiomotin Localizes Hippo Signaling in Preimplantation Embryos

  Yoshikazu Hirate; Shino Hirahara; Ken-ichi Inoue; Atsushi Suzuki; Vernadeth B. Alarcon; Kazunori Akimoto; Takaaki Hirai; Takeshi Hara; Makoto Adachi; Kazuhiro Chida; Shigeo Ohno; Yusuke Marikawa; Kazuki Nakao; Akihiko Shimono; Hiroshi Sasaki

  CURRENT BIOLOGY, Jul. 2013, Peer-reviewed

  
• Tara up-regulates E-cadherin transcription by binding to the Trio RhoGEF and inhibiting Rac signaling

  Tomoki Yano; Yuji Yamazaki; Makoto Adachi; Katsuya Okawa; Philippe Fort; Masami Uji; Shoichiro Tsukita; Sachiko Tsukita

  JOURNAL OF CELL BIOLOGY, Apr. 2011, Peer-reviewed

  
• Similar and Distinct Properties of MUPP1 and Patj, Two Homologous PDZ Domain-Containing Tight-Junction Proteins

  Makoto Adachi; Yoko Hamazaki; Yuka Kobayashi; Masahiko Itoh; Sachiko Tsukita; Mikio Furuse; Shoichiro Tsukita

  MOLECULAR AND CELLULAR BIOLOGY, May 2009, Peer-reviewed

  
• IQGAP3 regulates cell proliferation through the Ras/ERK signalling cascade

  Hisashi Nojima; Makoto Adachi; Takeshi Matsui; Katsuya Okawa; Shoichiro Tsukita; Sachiko Tsukita

  NATURE CELL BIOLOGY, Aug. 2008, Peer-reviewed

  
• Identification of adherens junction-associated GTPase activating proteins by the fluorescence localization-based expression cloning

  Miho Matsuda; Yuka Kobayashi; Sayuri Masuda; Makoto Adachi; Tsuyoshi Watanabe; Jun K. Yamashita; Eiichiro Nishi; Shoichiro Tsukita; Mikio Furuse

  EXPERIMENTAL CELL RESEARCH, Mar. 2008, Peer-reviewed

  
• Molecular characterization of angiomotin/JEAP family proteins: interaction with MUPP1/Patj and their endogenous properties

  Yuko Sugihara-Mizuno; Makoto Adachi; Yuka Kobayashi; Yoko Hamazaki; Miyuki Nishimura; Toshio Imai; Mikio Furuse; Shoichiro Tsukita

  GENES TO CELLS, Apr. 2007, Peer-reviewed

  
• Normal establishment of epithelial tight junctions in mice and cultured cells lacking expression of ZO-3, a tight-junction MAGUK protein

  Makoto Adachi; Akihito Inoko; Masaki Hata; Kyoko Furuse; Kazuaki Umeda; Masahiko Itoh; Shoichiro Tsukita

  MOLECULAR AND CELLULAR BIOLOGY, Dec. 2006, Peer-reviewed

  
• The Caenorhabditis elegans MAPK phosphatase VHP-1 mediates a novel JNK-like signaling pathway in stress response

  T Mizuno; N Hisamoto; T Terada; T Kondo; M Adachi; E Nishida; DH Kim; FM Ausubel; K Matsumoto

  EMBO JOURNAL, Jun. 2004, Peer-reviewed

  
• Subcellular localization of MAPKs.

  M. Adachi; E. Nishida

  Methods in molecular biology (Clifton, N.J.), 2004, Peer-reviewed

  
• Identification of a docking groove on ERK and p38 MAP kinases that regulates the specificity of docking interactions

  T Tanoue; R Maeda; M Adachi; E Nishida

  EMBO JOURNAL, Feb. 2001, Peer-reviewed

  
• Identification and characterization of a novel MAP kinase kinase kinase, MLTK

  Gotoh, I; M Adachi; E Nishida

  JOURNAL OF BIOLOGICAL CHEMISTRY, Feb. 2001, Peer-reviewed

  
• Nuclear export of MAP kinase (ERK) involves a MAP kinase kinase (MEK)-dependent active transport mechanism

  M Adachi; M Fukuda; E Nishida

  JOURNAL OF CELL BIOLOGY, Mar. 2000, Peer-reviewed

  
• A conserved docking motif in MAP kinases common to substrates, activators and regulators

  T Tanoue; M Adachi; T Moriguchi; E Nishida

  NATURE CELL BIOLOGY, Feb. 2000, Peer-reviewed

  
• Control of the cell morphology and the S phase entry by mitogen-activated protein kinase kinase - A regulatory role of its N-terminal region

  Gotoh, I; M Fukuda; M Adachi; E Nishida

  JOURNAL OF BIOLOGICAL CHEMISTRY, Apr. 1999, Peer-reviewed

  
• Two co-existing mechanisms for nuclear import of MAP kinase: passive diffusion of a monomer and active transport of a dimer

  M Adachi; M Fukuda; E Nishida

  EMBO JOURNAL, Oct. 1999, Peer-reviewed

  
• A novel regulatory mechanism in the mitogen-activated protein (MAP) kinase cascade - Role of nuclear export signal of MAP kinase kinase

  M Fukuda; Gotoh, I; M Adachi; Y Gotoh; E Nishida

  JOURNAL OF BIOLOGICAL CHEMISTRY, Dec. 1997, Peer-reviewed

  
• CRM1 is responsible for intracellular transport mediated by the nuclear export signal

  M Fukuda; S Asano; T Nakamura; M Adachi; M Yoshida; M Yanagida; E Nishida

  NATURE, Nov. 1997, Peer-reviewed

  

Misc.

• Nuclear export of MAP kinase (ERR) involves a MAP kinase (MEK)-dependent active transport mechanism (vol 148, pg 849, 2000)

  M Adachi; M Fukuda; E Nishida

  JOURNAL OF CELL BIOLOGY, May 2000

  
• 哺乳類細胞における細胞質分裂へのIQGAPファミリー分子の関与

  ADACHI MAKOTO; KAWASAKI ASAMI; NOJIMA HISASHI; NISHIDA EISUKE; TSUKITA SACHIKO

  日本分子生物学会年会プログラム・要旨集(Web), 2013

  
• Rac1を介したE‐cadherin転写制御因子としてのadherens junction構成タンパク質Tara/TRIOBP1の機能解析

  YANO TOMOKI; YAMAZAKI YUJI; ADACHI MAKOTO; OKAWA KATSUYA; FORT PHILIPPE; TSUKITA SHOICHIRO; TSUKITA SACHIKO

  生化学, 2011

  
• 細胞質分裂におけるIQGAP3の関与

  KAWASAKI ASAMI; ADACHI MAKOTO; NOJIMA HISASHI; KUNIMOTO KOUSHI; YAMASAKI YUJI; TAMURA ATSUSHI; TSUKITA SACHIKO

  生化学, 2010

  
• Rac-GEF-binding Tara builds E-cadherin/Ankyrin/Spectrin frameworks in the junctional circunferential ring.

  YANO TOMOKI; YAMAZAKI YUJI; ADACHI MAKOTO; OKAWA KATSUYA; TSUKITA SHOICHIRO; TSUKITA SACHIKO

  生化学, 25 Sep. 2009

  
• タイトジャンクションに局在する2つの類似したPDZドメイン含有分子MUPP1とPatjの解析

  ADACHI MAKOTO; HAMAZAKI YOKO; KOBAYASHI YUKA; TSUKITA SACHIKO; FURUSE MIKIO; TSUKITA SHOICHIRO

  生化学, 2007

  
• MKP‐1を介したMAPキナーゼ経路とNotch経路のクロストークの解析

  KONDO KUNIO; ADACHI MAKOTO; SUNADOME KAZUNORI; NISHIDA EISUKE

  日本分子生物学会年会講演要旨集, 25 Nov. 2005

  
• 線虫MAPキナーゼホスファターゼVHP‐1はストレス応答において新規JNK様シグナル伝達経路を制御する

  MIZUNO TOMOAKI; HISAMOTO NAOKI; TERADA TAKASHI; KONDO TAE; ADACHI MAKOTO; NISHIDA EISUKE; MATSUMOTO KUNIHIRO

  日本分子生物学会年会プログラム・講演要旨集, 25 Nov. 2004

  
• NotchによるMKP‐1の誘導とC2C12細胞の筋分化

  KONDO KUNIO; ADACHI MAKOTO; NISHIDA EISUKE

  日本細胞生物学会大会講演要旨集, 2003

  
• 新規MAPKKKの同定,活性化機構および機能の解析

  GOTO ISAMU; ADACHI MAKOTO; NISHIDA EISUKE

  日本分子生物学会年会プログラム・講演要旨集, 25 Nov. 2000

  
• 情報伝達研究の最前線 情報伝達の基本経路 MAPキナーゼファミリー

  ADACHI MAKOTO; NISHIDA EISUKE

  医学のあゆみ, 29 Jul. 2000

  
• MAPキナーゼの核移行および核外移行の制御機構

  ADACHI MAKOTO; FUKUDA MAKOTO; NISHIDA EISUKE

  日本分子生物学会年会プログラム・講演要旨集, 22 Nov. 1999

  
• MAPキナーゼファミリーの分子認識機構の解析

  TANOUE TAKUJI; ADACHI MAKOTO; MORIGUCHI TETSUO; NISHIDA EISUKE

  日本分子生物学会年会プログラム・講演要旨集, 22 Nov. 1999

  
• Regulation of the nucleocytoplasmic transport of MAP kinases.

  ADACHI MAKOTO; FUKUDA MAKOTO; NISHIDA EISUKE

  実験医学, Feb. 1999

  
• Regulation of Nucleocytoplasmic Transport of MAP kinase.

  ADACHI MAKOTO; FUKUDA MAKOTO; NISHIDA EISUKE

  日本分子生物学会年会プログラム・講演要旨集, Dec. 1997

  
• Molecular mechanisms of nucleocytoplasmic transport of MAP kinase kinase and MAP kinase.

  NISHIDA EISUKE; FUKUDA MAKOTO; GOTO ISAMU; ASANO SHIRO; ADACHI MAKOTO; WATANABE MINA

  日本分子生物学会年会プログラム・講演要旨集, Dec. 1997

  
• MAPキナーゼキナーゼの核外移行シグナル(NES)の役割および核外移行の分子機構の解析

  FUKUDA MAKOTO; GOTO ISAMU; ASANO SHIRO; ADACHI MAKOTO; NISHIDA EISUKE

  日本細胞生物学会大会講演要旨集, 1997

  

External funds: Kakenhi

• Novel approach focusing on the tight junction-based paracellular barrier combined with the apical barrier toward understanding and manipulating epithelial barriers

  Grant-in-Aid for Specially Promoted Research

  Biological Sciences

  Teikyo University;Osaka University

  月田 早智子

  From 23 Apr. 2019, To 31 Mar. 2024, Granted

  上皮バリア;タイトジャンクション;クローディン;上皮細胞;細胞骨格;アピカル膜;アピカル骨格;クライオ電顕;TJストランド;細胞間バリア;TJ-アピカル複合体;アピカル細胞骨格;TJ-ストランド;TJ-MAPs

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H05468/
• Elucidation of novel signaling mechanisms that regulates cell proliferation and division

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Makoto ADACHI

  From 01 Apr. 2016, To 31 Mar. 2019, Project Closed

  細胞内シグナル伝達機構;細胞内シグナル伝達;細胞質分裂

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K08582/
• Analysis of novel signaling pathways that regulate mammalian cell cytokinesis

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  makoto adachi

  From 01 Apr. 2013, To 31 Mar. 2016, Project Closed

  細胞質分裂;細胞内シグナル伝達

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25460362/
• Analysis of a novel signaling network that regulates cell growth and division

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  Makoto ADACHI

  From 01 Apr. 2011, To 31 Mar. 2013, Project Closed

  細胞内シグナル伝達;細胞質分裂;細胞増殖;X線結晶構造解析;遺伝子発現制御;癌

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790331/
• Functional analysis of novel components that localize to cell-cell adhesive structures in epithelial cells

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  Makoto ADACHI

  From 01 Apr. 2007, To 31 Mar. 2009, Project Closed

  細胞内・細胞間情報伝達;細胞接着;極性形成;細胞増殖;上皮細胞;接着構造形成;タイトジャンクション

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19770168/
• Molecular mechanisms of cell-cell adhesion in cancer cells

  Grant-in-Aid for Scientific Research on Priority Areas

  Biological Sciences

  Kobe University;Kyoto University

  Mikio FURUSE

  From 01 Apr. 2005, To 31 Mar. 2010, Project Closed

  上皮細胞;細胞接着;タイトジャンクション;クローディン;細胞間接着;血管内皮細胞;腸管バリア;近位尿細管;ユビキチン化;ZO-1;アドヘレンスジャンクション;ERM;マーリン;アドへレンスジャンクション;がん;ERM蛋白質;メルリン;トリセルリン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17014044/
• 上皮細胞の極性形成に関与するaPKCの生理的基質の探索

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  足立 誠

  From 01 Apr. 2002, To 31 Mar. 2004, Project Closed

  極性形成;上皮細胞;aPKC;ホスファターゼ;極性;キナーゼ;基質;タイトジャンクション

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14780543/
• Claudins in the epithelium/endothelium barrier dysfucrition

  Grant-in-Aid for Scientific Research (A)

  KYOTO UNIVERSITY

  Shoichiro TSUKITA

  From 01 Apr. 2002, To 31 Mar. 2005, Project Closed

  細胞間接着;タイトジャンクション;クローディン;オクルディン;上皮間バリアー;血液脳関門;ノックアウトマウス;コンパートメント;上皮細胞;内皮細胞;バリアー;ZO-1;ZO-2;血管;Zo-1;ノックアウト;裏打ち蛋白質;PDZドメイン;細胞接着, cell-cell adhesion;tight junction;claudin;occludin;epidermal barrier;blood-brain barrier;knockout mouse;compartment

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14207008/
• Molecular mechanism for cell-cell adhesion in canceration and metastasis

  Grant-in-Aid for Scientific Research on Priority Areas

  Biological Sciences

  KYOTO UNIVERSITY

  Shoichiro TSUKITA

  From 01 Apr. 2000, To 31 Mar. 2005, Project Closed

  細胞間接着;タイトジャンクション;クローディン;オクルディン;ERM蛋白質;上皮間バリアー;ノックアウトマウス;細胞増殖;Snail;ZO-1;ZO-2;がん;上皮間葉転換;転移浸潤;GFP;ドラッグデリバリー;ノックアウト;上皮細胞;血液脳関門;メルリン;MUPP1;PDZドメイン;大腸癌;ラディキシン;α-カテニン;β-カテニン;ERM;アドヘレンスジャンクション;カドヘリン, cell-cell adhesion;tight junction;claudin;occludin;ERM proteins;epidermal barrier;knockout mouse;cell proliferation

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12219210/
• Novel approach focusing on the tight junction-based paracellular barrier combined with the apical barrier toward understanding and manipulating epithelial barriers

  Grant-in-Aid for Specially Promoted Research

  Biological Sciences

  Teikyo University;Osaka University

  月田 早智子

  From 23 Apr. 2019, To 31 Mar. 2024, Granted

  上皮バリア;タイトジャンクション;クローディン;上皮細胞;細胞骨格;クライオ電顕トモグラフィー;アピカル膜;液-液相分離（LLPS）;TJMAP;アピカル骨格;クライオ電顕;TJストランド;細胞間バリア;TJ-アピカル複合体;アピカル細胞骨格;TJ-ストランド;TJ-MAPs

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H05468/

External funds: others

• Functional analysis of novel components that localize to cell-cell adhesion structure in epithelial cells

  足立　誠

Administration

Faculty management (title, position)

• 安全衛生委員会(委員)
• 化学物質管理運営委員会(委員)
• 図書委員委員(委員)
• To 31 Mar. 2019

  医学図書館図書連絡委員会