Hotta, Akitsu(Center for iPS Cell Research and Application (CiRA)) | Activity Database on Education and Research, Kyoto University

Hotta, Akitsu

Center for iPS Cell Research and Application (CiRA) Associate Professor

Last Updated :2025/04/23

Basic Information

Concurrent Affiliation

Center for iPS Cell Research and Application (CiRA), 初期化機構研究部門, 特定拠点講師

Center for iPS Cell Research and Application (CiRA), 臨床応用研究部門, 特定拠点講師

Professional Memberships

日本筋学会

THE JAPANESE SOCIETY OF GENOME EDITING

American Society for Gene and Cell Therapy (ASGCT)

International Society for Stem Cell Research (ISSCR)

JAPAN SOCIETY OF GENE AND CELL THERAPY

THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE

THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

Academic Degree

修士（工学）(名古屋大学)

博士（工学）(名古屋大学)

Academic Resume (Graduate Schools)

Nagoya University, 大学院工学研究科博士課程後期課程生物機能工学専攻, 修了

Nagoya University, 大学院工学研究科博士課程前期課程生物機能工学専攻, 修了

Academic Resume (Undergraduate School/Majors)

Nagoya University, 工学部化学･生物工学科, 卒業

High School

High School
名古屋大学教育学部附属高等学校

Research History

From May 2022, To Present
Center for iPS cell Research and Application, Kyoto University, Department of Clinical Application, Associate Professor

From Oct. 2020, To Apr. 2022
Center for iPS cell Research and Application, Kyoto University, Department of Clinical Application, Junior Associate Professor

From Apr. 2016, To Present
T-CiRA Program, Hotta Project, Project Leader

From Apr. 2018, To Sep. 2020
Center for iPS cell Research and Application, Kyoto University, Department of Clinical Application, Juniro Associate Professor

From Oct. 2016, To Mar. 2018
Center for iPS cell Research and Application, Kyoto University, Department of Life Science Frontiers, Junior Associate Professor

From Jun. 2015, To Sep. 2016
京都大学iPS細胞研究所, 未来生命科学開拓部門, 特定拠点助教

From Mar. 2010, To May 2015
Center for iPS Cell Research and application, Kyoto University, 初期化機構研究部門, Assistant Professor

From Jun. 2008, To Feb. 2010
Ontario Human iPS Cell Facility, Research Fellow

From Apr. 2006, To Feb. 2010
The Hospital for Sick Children, Developmental & Stem Cell Biology, Postdoctoral Research Fellow

Profile

Profile
現在の医学でも治療できない難病の半分以上は、遺伝子変異が原因で引き起こされます。遺伝子は生物の設計図であり、これを細胞内・生体内で自在に改変することが可能となれば、遺伝子変異難病の治療に繋がると考えています。CRISPR-Casシステムを中心としたゲノム編集技術、ウイルスベクターやトランスポゾンベクターによる遺伝子導入ベクター、患者さん自身のiPS細胞、そしてゲノム編集酵素を自在に生体内の組織へ送り届ける送達技術を駆使して、新しい"ゲノム編集治療"の実現に向けた研究に取り組んでいます。

Language of Instruction

English

Last Updated :2025/04/23

Research

Research Topics, Overview of the research

Research Topics
Gene Engineering and Gene Therapy of iPS cells
Overview of the research
iPS cell technology provides a way to isolate any cell type from a donor in a minimally invasive fashion. Two attractive features of iPS cells are that they can grow indefinitely on culture dish by keeping their pluripotency and be differentiated into any cell type. Thus, iPS cells have enormous potential for drug testing and future stem cell therapy. They also offer transplantation that is free of immune rejection and independent of donor supply. However, when iPS cells are derived from patients with congenital genetic disorders, such as hemophilia or muscular dystrophy, the genetic mutations must be repaired before transplantation. For this reason, our research group aims to develop novel iPS cell based therapies for genetic disorders using non-viral transposon-mediated gene delivery methods and genome editing technologies, such as the CRISPR-Cas9 system.

Research Interests

iPS cells

CRISPR

Muscular dystrophy

Genome editing

エピジェネティクス

幹細胞

遺伝子治療

ウイルスベクター

Research Areas

Life sciences, Genomics

Life sciences, Medical biochemistry, Gene Therapy

Life sciences, Developmental biology

Life sciences, Cell biology

Life sciences, Molecular biology

Papers

デュシェンヌ型筋ジストロフィー治療に向けた新規CRISPR-Cas3システムの開発(Developing a novel CRISPR-Cas3 system for Duchenne muscular dystrophy therapy)
北悠人; 奥嵜雄也; Gee Peter; 徐淮耕; 笹川典子; 吉見一人; 竹田潤二; 真下知士; 堀田秋津
日本細胞生物学会大会講演要旨集, Jun. 2020

Genetically engineered hypoimmunogenic cell therapy
Akitsu Hotta; Sonja Schrepfer; Andras Nagy
Nature Reviews Bioengineering, 29 Jul. 2024, Peer-reviewed, Invited, Lead author, Corresponding author

Optimized simple culture protocol for inducing mature myotubes from MYOD1-overexpressed human iPS cells.
Eiji Wada; Nao Susumu; Yuya Okuzaki; Akitsu Hotta; Hidetoshi Sakurai; Yukiko K Hayashi
Scientific reports, 20 Nov. 2024

iMSC-mediated delivery of ACVR2B-Fc fusion protein reduces heterotopic ossification in a mouse model of fibrodysplasia ossificans progressiva.
Pan Gao; Yoshiko Inada; Akitsu Hotta; Hidetoshi Sakurai; Makoto Ikeya
Stem cell research & therapy, 18 Mar. 2024
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Beneath the radar: immune-evasive cell sources for stroke therapy.
Beatriz Achón Buil; Nora H Rentsch; Rebecca Z Weber; Chiara Rickenbach; Stefanie J Halliday; Akitsu Hotta; Christian Tackenberg; Ruslan Rust
Trends in molecular medicine, Mar. 2024

Hiding from allogeneic NK cells and macrophages by a synthetic receptor.
Akitsu Hotta; Joseph Lee
Cell Stem Cell, 02 Nov. 2023, Invited, Lead author, Corresponding author

iPSC-derived type IV collagen α5-expressing kidney organoids model Alport syndrome
Ryuichiro Hirayama; Kosuke Toyohara; Kei Watanabe; Takeya Otsuki; Toshikazu Araoka; Shin-Ichi Mae; Tomoko Horinouchi; Tomohiko Yamamura; Keisuke Okita; Akitsu Hotta; Kazumoto Iijima; Kandai Nozu; Kenji Osafune
Communications Biology, 28 Sep. 2023
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Neutralization sensitivity of FL.1 and GE.1 from therapeutic antibodies and seropositive XBB sera
Joseph Lee; Youichi Naoe; Uikyu Bang; Yu Nakagama; Akatsuki Saito; Yasutoshi Kido; Akitsu Hotta
Virology, Apr. 2024, Peer-reviewed, Corresponding author
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CD151 expression marks atrial- and ventricular- differentiation from human induced pluripotent stem cells.
Misato Nakanishi-Koakutsu; Kenji Miki; Yuki Naka; Masako Sasaki; Takayuki Wakimizu; Stephanie C Napier; Chikako Okubo; Megumi Narita; Misato Nishikawa; Reo Hata; Kazuhisa Chonabayashi; Akitsu Hotta; Kenichi Imahashi; Tomoyuki Nishimoto; Yoshinori Yoshida
Communications biology, 28 Feb. 2024
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Dual CRISPR-Cas3 system for inducing multi-exon skipping in DMD patient-derived iPSCs
Yuto Kita; Yuya Okuzaki; Youichi Naoe; Joseph Lee; Uikyu Bang; Natsumi Okawa; Akane Ichiki; Tatsuya Jonouchi; Hidetoshi Sakurai; Yusuke Kojima; Akitsu Hotta
Stem Cell Reports, Aug. 2023, Peer-reviewed, Corresponding author
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Humanized mouse models with endogenously developed human natural killer cells for in vivo immunogenicity testing of HLA class I-edited iPSC-derived cells.
Charlotte Flahou; Tatsuya Morishima; Natsumi Higashi; Yoshikazu Hayashi; Huaigeng Xu; Bo Wang; Chaoqi Zhang; Atsushi Ninomiya; Wei-Yin Qiu; Akinori Yuzuriha; Daisuke Suzuki; Sou Nakamura; Markus Manz; Shin Kaneko; Akitsu Hotta; Hitoshi Takizawa; Koji Eto; Naoshi Sugimoto
Biochemical and biophysical research communications, 20 Apr. 2023

Establishment of quantitative and consistent in vitro skeletal muscle pathological models of myotonic dystrophy type 1 using patient-derived iPSCs.
Ryu Kawada; Tatsuya Jonouchi; Akihiro Kagita; Masae Sato; Akitsu Hotta; Hidetoshi Sakurai
Scientific reports, 11 Jan. 2023
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Preparation of NanoMEDIC Extracellular Vesicles to Deliver CRISPR-Cas9 Ribonucleoproteins for Genomic Exon Skipping.
Kei Watanabe; Peter Gee; Akitsu Hotta
Methods in molecular biology (Clifton, N.J.), 2023

Optimization of the proliferation and persistency of CAR T cells derived from human induced pluripotent stem cells.
Tatsuki Ueda; Sara Shiina; Shoichi Iriguchi; Seitaro Terakura; Yohei Kawai; Ryotaro Kabai; Satoko Sakamoto; Akira Watanabe; Kohei Ohara; Bo Wang; Huaigeng Xu; Atsutaka Minagawa; Akitsu Hotta; Knut Woltjen; Yasushi Uemura; Yuzo Kodama; Hiroshi Seno; Tetsuya Nakatsura; Koji Tamada; Shin Kaneko
Nature biomedical engineering, Jan. 2023
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Generation of hypoimmunogenic induced pluripotent stem cells by CRISPR-Cas9 system and detailed evaluation for clinical application.
Yuko Kitano; Sayaka Nishimura; Tomoaki M Kato; Anna Ueda; Kaho Takigawa; Masafumi Umekage; Masaki Nomura; Ayane Kawakami; Haruna Ogawa; Huaigeng Xu; Akitsu Hotta; Naoko Takasu; Masayoshi Tsukahara
Molecular therapy. Methods & clinical development, 08 Sep. 2022
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is a therapeutic target in SPG4-related hereditary spastic paraplegia human neurons.
Fumiko Nakazeki; Itaru Tsuge; Takahiro Horie; Keiko Imamura; Kayoko Tsukita; Akitsu Hotta; Osamu Baba; Yasuhide Kuwabara; Tomohiro Nishino; Tetsushi Nakao; Masataka Nishiga; Hitoo Nishi; Yasuhiro Nakashima; Yuya Ide; Satoshi Koyama; Masahiro Kimura; Shuhei Tsuji; Motoko Naitoh; Shigehiko Suzuki; Yuishin Izumi; Toshitaka Kawarai; Ryuji Kaji; Takeshi Kimura; Haruhisa Inoue; Koh Ono
Clinical Science, 22 Feb. 2019, Peer-reviewed

Direct visualization of the transition status during neural differentiation by dual-fluorescent reporter human pluripotent stem cells
Gwanghyun Park; Minkyung Shin; Wonyoung Lee; Akitsu Hotta; Taeko Kobayashi; Yoichi Kosodo
Stem Cell Reports, Aug. 2022

iPSC-based drug repositioning identifies Src/c-Abl as a therapeutic target for ALS motor neurons.
Imamura K; Izumi Y; Watanabe A; Tsukita K; Woltjen K; Yamamoto T; Hotta A; Kondo T; Kitaoka S; Ohta A; Tanaka A; Watanabe D; Morita M; Kaji R; Takuma H; Tamaoka A; Kunath T; Wray S; Furuya H; Era T; Fujisawa T; Nishotoh H; Kengo H; Ichijo H; Julien J-P; Obata N; Hosokawa M; Akiyama H; Ayaki T; Ito H; Takahashi R; Yamanaka S; Inoue H
Science Translational Medicine, May 2017, Peer-reviewed

Development of alternative gene transfer techniques for ex vivo and in vivo gene therapy in a canine model.
Masashi Noda; Kohei Tatsumi; Hideto Matsui; Yasunori Matsunari; Takeshi Sato; Yasushi Fukuoka; Akitsu Hotta; Teruo Okano; Kimihiko Kichikawa; Mitsuhiko Sugimoto; Midori Shima; Kenji Nishio
Regenerative therapy, Dec. 2021

Expression dynamics of HAND1/2 in in vitro human cardiomyocyte differentiation.
Chikako Okubo; Megumi Narita; Azusa Inagaki; Misato Nishikawa; Akitsu Hotta; Shinya Yamanaka; Yoshinori Yoshida
Stem cell reports, 10 Aug. 2021, Peer-reviewed
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A muscle fatigue-like contractile decline was recapitulated using skeletal myotubes from Duchenne muscular dystrophy patient-derived iPSCs.
Tomoya Uchimura; Toshifumi Asano; Takao Nakata; Akitsu Hotta; Hidetoshi Sakurai
Cell reports Medicine, 15 Jun. 2021, Peer-reviewed
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Generation of hypoimmunogenic T cells from genetically engineered allogeneic human induced pluripotent stem cells.
Bo Wang; Shoichi Iriguchi; Masazumi Waseda; Norihiro Ueda; Tatsuki Ueda; Huaigeng Xu; Atsutaka Minagawa; Akihiro Ishikawa; Hisashi Yano; Tomoko Ishi; Ryoji Ito; Motohito Goto; Riichi Takahashi; Yasushi Uemura; Akitsu Hotta; Shin Kaneko
Nature biomedical engineering, May 2021, Peer-reviewed

Efficient ssODN-Mediated Targeting by Avoiding Cellular Inhibitory RNAs through Precomplexed CRISPR-Cas9/sgRNA Ribonucleoprotein
Akihiro Kagita; Mandy S.Y. Lung; Huaigeng Xu; Yuto Kita; Noriko Sasakawa; Takahiro Iguchi; Miyuki Ono; Xiou H. Wang; Peter Gee; Akitsu Hotta
Stem Cell Reports, Apr. 2021, Peer-reviewed, Last author, Corresponding author
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Characterization of hiPSC-Derived Muscle Progenitors Reveals Distinctive Markers for Myogenic Cell Purification Toward Cell Therapy.
Minas Nalbandian; Mingming Zhao; Mitsuru Sasaki-Honda; Tatsuya Jonouchi; Antonio Lucena-Cacace; Takuma Mizusawa; Masahiko Yasuda; Yoshinori Yoshida; Akitsu Hotta; Hidetoshi Sakurai
Stem cell reports, 17 Mar. 2021, Peer-reviewed
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MAGI-2 orchestrates the localization of backbone proteins in the slit diaphragm of podocytes.
Hiroyuki Yamada; Naritoshi Shirata; Shinichi Makino; Takafumi Miyake; Juan Alejandro Oliva Trejo; Kanae Yamamoto-Nonaka; Mitsuhiro Kikyo; Maulana A Empitu; Ika N Kadariswantiningsih; Maiko Kimura; Koichiro Ichimura; Hideki Yokoi; Masashi Mukoyama; Akitsu Hotta; Katsuhiko Nishimori; Motoko Yanagita; Katsuhiko Asanuma
Kidney international, Feb. 2021, Peer-reviewed

PKD1-Dependent Renal Cystogenesis in Human Induced Pluripotent Stem Cell-Derived Ureteric Bud/Collecting Duct Organoids
Shohei Kuraoka; Shunsuke Tanigawa; Atsuhiro Taguchi; Akitsu Hotta; Hitoshi Nakazato; Kenji Osafune; Akio Kobayashi; Ryuichi Nishinakamura
Journal of the American Society of Nephrology, Oct. 2020, Peer-reviewed

A novel ADPKD model using kidney organoids derived from disease-specific human iPSCs.
Tatsuya Shimizu; Shin-Ichi Mae; Toshikazu Araoka; Keisuke Okita; Akitsu Hotta; Kunihiro Yamagata; Kenji Osafune
Biochemical and biophysical research communications, 03 Sep. 2020, Peer-reviewed

Induced Fetal Human Muscle Stem Cells with High Therapeutic Potential in a Mouse Muscular Dystrophy Model.
Mingming Zhao; Atsutoshi Tazumi; Satoru Takayama; Nana Takenaka-Ninagawa; Minas Nalbandian; Miki Nagai; Yumi Nakamura; Masanori Nakasa; Akira Watanabe; Makoto Ikeya; Akitsu Hotta; Yuta Ito; Takahiko Sato; Hidetoshi Sakurai
Stem cell reports, 14 Jul. 2020, Peer-reviewed
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Generation of a transgene-free iPSC line and genetically modified line from a facioscapulohumeral muscular dystrophy type 2 (FSHD2) patient with SMCHD1 p.Lys607Ter mutation.
Mitsuru Sasaki-Honda; Akihiro Kagita; Tatsuya Jonouchi; Toshiyuki Araki; Akitsu Hotta; Hidetoshi Sakurai
Stem cell research, 24 Jun. 2020, Peer-reviewed
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Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping.
Peter Gee; Mandy S Y Lung; Yuya Okuzaki; Noriko Sasakawa; Takahiro Iguchi; Yukimasa Makita; Hiroyuki Hozumi; Yasutomo Miura; Lucy F Yang; Mio Iwasaki; Xiou H Wang; Matthew A Waller; Nanako Shirai; Yasuko O Abe; Yoko Fujita; Kei Watanabe; Akihiro Kagita; Kumiko A Iwabuchi; Masahiko Yasuda; Huaigeng Xu; Takeshi Noda; Jun Komano; Hidetoshi Sakurai; Naoto Inukai; Akitsu Hotta
Nature communications, 13 Mar. 2020, Peer-reviewed, Last author, Corresponding author
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Tissue-Engineered Vascular Grafts with Advanced Mechanical Strength from Human iPSCs.
Jiesi Luo; Lingfeng Qin; Liping Zhao; Liqiong Gui; Matthew W Ellis; Yan Huang; Mehmet H Kural; J Alexander Clark; Shun Ono; Juan Wang; Yifan Yuan; Shang-Min Zhang; Xiaoqiang Cong; Guangxin Li; Muhammad Riaz; Colleen Lopez; Akitsu Hotta; Stuart Campbell; George Tellides; Alan Dardik; Laura E Niklason; Yibing Qyang
Cell stem cell, 06 Feb. 2020, Peer-reviewed

iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity.
Daisuke Suzuki; Charlotte Flahou; Norihide Yoshikawa; Ieva Stirblyte; Yoshikazu Hayashi; Akira Sawaguchi; Marina Akasaka; Sou Nakamura; Natsumi Higashi; Huaigeng Xu; Takuya Matsumoto; Kosuke Fujio; Markus G Manz; Akitsu Hotta; Hitoshi Takizawa; Koji Eto; Naoshi Sugimoto
Stem cell reports, 14 Jan. 2020, Peer-reviewed
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CRISPR-Cas3 induces broad and unidirectional genome editing in human cells.
Hiroyuki Morisaka; Kazuto Yoshimi; Yuya Okuzaki; Peter Gee; Yayoi Kunihiro; Ekasit Sonpho; Huaigeng Xu; Noriko Sasakawa; Yuki Naito; Shinichiro Nakada; Takashi Yamamoto; Shigetoshi Sano; Akitsu Hotta; Junji Takeda; Tomoji Mashimo
Nature communications, 06 Dec. 2019, Peer-reviewed, Corresponding author
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In Vitro Disease Modeling of Hermansky-Pudlak Syndrome Type 2 Using Human Induced Pluripotent Stem Cell-Derived Alveolar Organoids.
Yohei Korogi; Shimpei Gotoh; Satoshi Ikeo; Yuki Yamamoto; Naoyuki Sone; Koji Tamai; Satoshi Konishi; Tadao Nagasaki; Hisako Matsumoto; Isao Ito; Toyofumi F Chen-Yoshikawa; Hiroshi Date; Masatoshi Hagiwara; Isao Asaka; Akitsu Hotta; Michiaki Mishima; Toyohiro Hirai
Stem cell reports, 09 Jul. 2019

Targeted Disruption of HLA Genes via CRISPR-Cas9 Generates iPSCs with Enhanced Immune Compatibility.
Huaigeng Xu; Bo Wang; Miyuki Ono; Akihiro Kagita; Kaho Fujii; Noriko Sasakawa; Tatsuki Ueda; Peter Gee; Misato Nishikawa; Masaki Nomura; Fumiyo Kitaoka; Tomoko Takahashi; Keisuke Okita; Yoshinori Yoshida; Shin Kaneko; Akitsu Hotta
Cell stem cell, 04 Apr. 2019, Peer-reviewed, Last author, Corresponding author

In Vitro Disease Modeling of Hermansky-Pudlak Syndrome Type 2 Using Human Induced Pluripotent Stem Cell-Derived Alveolar Organoids.
Yohei Korogi; Shimpei Gotoh; Satoshi Ikeo; Yuki Yamamoto; Naoyuki Sone; Koji Tamai; Satoshi Konishi; Tadao Nagasaki; Hisako Matsumoto; Isao Ito; Toyofumi F Chen-Yoshikawa; Hiroshi Date; Masatoshi Hagiwara; Isao Asaka; Akitsu Hotta; Michiaki Mishima; Toyohiro Hirai
Stem cell reports, 05 Mar. 2019, Peer-reviewed
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MiR-33a is a therapeutic target in SPG4-related hereditary spastic paraplegia human neurons.
Nakazeki F; Tsuge I; Horie T; Imamura K; Tsukita K; Hotta A; Baba O; Kuwabara Y; Nishino T; Nakao T; Nishiga M; Nishi H; Nakashima Y; Ide Y; Koyama S; Kimura M; Tsuji S; Naitoh M; Suzuki S; Izumi Y; Kawarai T; Kaji R; Kimura T; Inoue H; Ono K
Clinical Science (London), Feb. 2019, Peer-reviewed

Enhancing T Cell Receptor Stability in Rejuvenated iPSC-Derived T Cells Improves Their Use in Cancer Immunotherapy.
Atsutaka Minagawa; Toshiaki Yoshikawa; Masaki Yasukawa; Akitsu Hotta; Mihoko Kunitomo; Shoichi Iriguchi; Maiko Takiguchi; Yoshiaki Kassai; Eri Imai; Yutaka Yasui; Yohei Kawai; Rong Zhang; Yasushi Uemura; Hiroyuki Miyoshi; Mahito Nakanishi; Akira Watanabe; Akira Hayashi; Kei Kawana; Tomoyuki Fujii; Tetsuya Nakatsura; Shin Kaneko
Cell stem cell, 06 Dec. 2018, Peer-reviewed

Site-specific randomization of the endogenous genome by a regulatable CRISPR-Cas9 piggyBac system in human cells
Kentaro Ishida; Huaigeng Xu; Noriko Sasakawa; Mandy Siu Yu Lung; Julia Alexandra Kudryashev; Peter Gee; Akitsu Hotta
Scientific Reports, 01 Dec. 2018, Peer-reviewed, Last author, Corresponding author
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A patient-derived iPSC model revealed oxidative stress increases facioscapulohumeral muscular dystrophy-causative DUX4.
Mitsuru Sasaki-Honda; Tatsuya Jonouchi; Meni Arai; Akitsu Hotta; Satomi Mitsuhashi; Ichizo Nishino; Ryoichi Matsuda; Hidetoshi Sakurai
Human molecular genetics, 01 Dec. 2018, Peer-reviewed
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Efficient mRNA delivery system utilizing chimeric VSVG-L7Ae virus-like particles.
Yulia Zhitnyuk; Peter Gee; Mandy S Y Lung; Noriko Sasakawa; Huaigeng Xu; Hirohide Saito; Akitsu Hotta
Biochemical and biophysical research communications, 10 Nov. 2018, Peer-reviewed, Last author, Corresponding author

A β1-tubulin-based megakaryocyte maturation reporter system identifies novel drugs that promote platelet production.
Hideya Seo; Si Jing Chen; Kazuya Hashimoto; Hiroshi Endo; Yohei Nishi; Akira Ohta; Takuya Yamamoto; Akitsu Hotta; Akira Sawaguchi; Hideki Hayashi; Noritaka Koseki; George J Murphy; Kazuhiko Fukuda; Naoshi Sugimoto; Koji Eto
Blood advances, 11 Sep. 2018, Peer-reviewed

Pluripotent Stem Cell Model of Nakajo-Nishimura Syndrome Untangles Proinflammatory Pathways Mediated by Oxidative Stress.
Fumiko Honda-Ozaki; Madoka Terashima; Akira Niwa; Norikazu Saiki; Yuri Kawasaki; Haruna Ito; Akitsu Hotta; Ayako Nagahashi; Koichi Igura; Isao Asaka; Hongmei Lisa Li; Masakatsu Yanagimachi; Fukumi Furukawa; Nobuo Kanazawa; Tatsutoshi Nakahata; Megumu K Saito
Stem cell reports, 05 Jun. 2018, Peer-reviewed
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Srf destabilizes cellular identity by suppressing cell-type-specific gene expression programs.
Takashi Ikeda; Takafusa Hikichi; Hisashi Miura; Hirofumi Shibata; Kanae Mitsunaga; Yosuke Yamada; Knut Woltjen; Kei Miyamoto; Ichiro Hiratani; Yasuhiro Yamada; Akitsu Hotta; Takuya Yamamoto; Keisuke Okita; Shinji Masui
Nature communications, 11 Apr. 2018, Peer-reviewed
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Human AK2 links intracellular bioenergetic redistribution to the fate of hematopoietic progenitors.
Koichi Oshima; Norikazu Saiki; Michihiro Tanaka; Hiromi Imamura; Akira Niwa; Ayako Tanimura; Ayako Nagahashi; Akiyoshi Hirayama; Keisuke Okita; Akitsu Hotta; Shuichi Kitayama; Mitsujiro Osawa; Shin Kaneko; Akira Watanabe; Isao Asaka; Wataru Fujibuchi; Kohsuke Imai; Hiromasa Yabe; Yoshiro Kamachi; Junichi Hara; Seiji Kojima; Masaru Tomita; Tomoyoshi Soga; Takafumi Noma; Shigeaki Nonoyama; Tatsutoshi Nakahata; Megumu K Saito
Biochemical and biophysical research communications, 04 Mar. 2018, Peer-reviewed
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Pluripotent stem cell models of Blau syndrome reveal an IFN-γ-dependent inflammatory response in macrophages.
Sanami Takada; Naotomo Kambe; Yuri Kawasaki; Akira Niwa; Fumiko Honda-Ozaki; Kazuki Kobayashi; Mitsujiro Osawa; Ayako Nagahashi; Katsunori Semi; Akitsu Hotta; Isao Asaka; Yasuhiro Yamada; Ryuta Nishikomori; Toshio Heike; Hiroyuki Matsue; Tatsutoshi Nakahata; Megumu K Saito
The Journal of allergy and clinical immunology, Jan. 2018, Peer-reviewed

Evolutionarily Distinctive Transcriptional and Signaling Programs Drive Human Germ Cell Lineage Specification from Pluripotent Stem Cells.
Yoji Kojima; Kotaro Sasaki; Shihori Yokobayashi; Yoshitake Sakai; Tomonori Nakamura; Yukihiro Yabuta; Fumio Nakaki; So Nagaoka; Knut Woltjen; Akitsu Hotta; Takuya Yamamoto; Mitinori Saitou
Cell stem cell, 05 Oct. 2017, Peer-reviewed

Reprogramming progeria fibroblasts re-establishes a normal epigenetic landscape
Zhaoyi Chen; Wing Y. Chang; Alton Etheridge; Hilmar Strickfaden; Zhigang Jin; Gareth Palidwor; Ji-Hoon Cho; Kai Wang; Sarah Y. Kwon; Carole Doré; Angela Raymond; Akitsu Hotta; James Ellis; Rita A. Kandel; F. Jeffrey Dilworth; Theodore J. Perkins; Michael J. Hendzel; David J. Galas; William L. Stanford
Aging Cell, 01 Aug. 2017, Peer-reviewed

Cell-type-specific genome editing with a microRNA-responsive CRISPR-Cas9 switch
Moe Hirosawa; Yoshihiko Fujita; Callum J. C. Parr; Karin Hayashi; Shunnichi Kashida; Akitsu Hotta; Knut Woltjen; Hirohide Saito
NUCLEIC ACIDS RESEARCH, Jul. 2017, Peer-reviewed
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Transplantation of neurons derived from human iPS cells cultured on collagen matrix into guinea-pig cochleae
Masaaki Ishikawa; Hiroe Ohnishi; Desislava Skerleva; Tatsunori Sakamoto; Norio Yamamoto; Akitsu Hotta; Juichi Ito; Takayuki Nakagawa
Journal of Tissue Engineering and Regenerative Medicine, Jun. 2017, Peer-reviewed

The Src/c-Abl pathway is a potential therapeutic target in amyotrophic lateral sclerosis
Keiko Imamura; Yuishin Izumi; Akira Watanabe; Kayoko Tsukita; Knut Woltjen; Takuya Yamamoto; Akitsu Hotta; Takayuki Kondo; Shiho Kitaoka; Akira Ohta; Akito Tanaka; Dai Watanabe; Mitsuya Morita; Hiroshi Takuma; Akira Tamaoka; Tilo Kunath; Selina Wray; Hirokazu Furuya; Takumi Era; Kouki Makioka; Koichi Okamoto; Takao Fujisawa; Hideki Nishitoh; Kengo Homma; Hidenori Ichijo; Jean-Pierre Julien; Nanako Obata; Masato Hosokawa; Haruhiko Akiyama; Satoshi Kaneko; Takashi Ayaki; Hidefumi Ito; Ryuji Kaji; Ryosuke Takahashi; Shinya Yamanaka; Haruhisa Inoue
Science Translational Medicine, 24 May 2017, Peer-reviewed

Calcium dysregulation contributes to neurodegeneration in FTLD patient iPSC-derived neurons
Keiko Imamura; Naruhiko Sahara; Nicholas M. Kanaan; Kayoko Tsukita; Takayuki Kondo; Yumiko Kutoku; Yutaka Ohsawa; Yoshihide Sunada; Koichi Kawakami; Akitsu Hotta; Satoshi Yawata; Dai Watanabe; Masato Hasegawa; John Q. Trojanowski; Virginia M.-Y. Lee; Tetsuya Suhara; Makoto Higuchi; Haruhisa Inoue
Scientific Reports, 10 Oct. 2016, Peer-reviewed
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Concordant but Varied Phenotypes among Duchenne Muscular Dystrophy Patient-Specific Myoblasts Derived using a Human iPSC-Based Model
In Young Choi; HoTae Lim; Kenneth Estrellas; Jyothi Mula; Tatiana V. Cohen; Yuanfan Zhang; Christopher J. Donnelly; Jean-Philippe Richard; Yong Jun Kim; Hyesoo Kim; Yasuhiro Kazuki; Mitsuo Oshimura; Hongmei Lisa Li; Akitsu Hotta; Jeffrey Rothstein; Nicholas Maragakis; Kathryn R. Wagner; Gabsang Lee
Cell reports, Jun. 2016, Peer-reviewed
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An EWS-FLI1 -Induced Osteosarcoma Model Unveiled a Crucial Role of Impaired Osteogenic Differentiation on Osteosarcoma Development
Shingo Komura; Katsunori Semi; Fumiaki Itakura; Hirofumi Shibata; Takatoshi Ohno; Akitsu Hotta; Knut Woltjen; Takuya Yamamoto; Haruhiko Akiyama; Yasuhiro Yamada
Stem Cell Reports, Apr. 2016, Peer-reviewed
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Sall1 transiently marks undifferentiated heart precursors and regulates their fate
Yuika Morita; Peter Andersen; Akitsu Hotta; Yuko Tsukahara; Noriko Sasagawa; Naoko Hayashida; Chizuko Koga; Misato Nishikawa; Yumiko Saga; Sylvia M. Evans; Kazuko Koshiba-Takeuchi; Ryuichi Nishinakamura; Yoshinori Yoshida; Chulan Kwon; Jun K. Takeuchi
Journal of Molecular and Cellular Cardiology, Mar. 2016, Peer-reviewed

Targeted gene correction of RUNX1 in induced pluripotent stem cells derived from familial platelet disorder with propensity to myeloid malignancy restores normal megakaryopoiesis
Hiromitsu Iizuka; Yuki Kagoya; Keisuke Kataoka; Akihide Yoshimi; Masashi Miyauchi; Kazuki Taoka; Keiki Kumano; Takashi Yamamoto; Akitsu Hotta; Shunya Arai; Mineo Kurokawa
Experimental Hematology, Oct. 2015, Peer-reviewed

Precise correction of the dystrophin gene in duchenne muscular dystrophy patient induced pluripotent stem cells by TALEN and CRISPR-Cas9.
Hongmei Lisa Li; Naoko Fujimoto; Noriko Sasakawa; Saya Shirai; Tokiko Ohkame; Tetsushi Sakuma; Michihiro Tanaka; Naoki Amano; Akira Watanabe; Hidetoshi Sakurai; Takashi Yamamoto; Shinya Yamanaka; Akitsu Hotta
Stem cell reports, 13 Jan. 2015, Peer-reviewed, Last author, Corresponding author
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Comprehensive chemical secretory measurement of single cells trapped in a micro-droplet array with mass spectrometry
Hideaki Fujita; Tsuyoshi Esaki; Tsutomu Masujima; Akitsu Hotta; Soo Hyeon Kim; Hiroyuki Noji; Tomonobu M. Watanabe
RSC Advances, 2015, Peer-reviewed

Hair Follicle Dermal Stem Cells Regenerate the Dermal Sheath, Repopulate the Dermal Papilla, and Modulate Hair Type
Waleed Rahmani; Sepideh Abbasi; Andrew Hagner; Eko Raharjo; Ranjan Kumar; Akitsu Hotta; Scott Magness; Daniel Metzger; Jeff Biernaskie
Developmental Cell, Dec. 2014, Peer-reviewed

Focal transplantation of human iPSC-derived glial-rich neural progenitors improves lifespan of ALS mice.
Takayuki Kondo; Misato Funayama; Kayoko Tsukita; Akitsu Hotta; Akimasa Yasuda; Satoshi Nori; Shinjiro Kaneko; Masaya Nakamura; Ryosuke Takahashi; Hideyuki Okano; Shinya Yamanaka; Haruhisa Inoue
Stem cell reports, 12 Aug. 2014, Peer-reviewed

Delivery of Full-Length Factor VIII Using a piggyBac Transposon Vector to Correct a Mouse Model of Hemophilia A
Hideto Matsui; Naoko Fujimoto; Noriko Sasakawa; Yasuhide Ohinata; Midori Shima; Shinya Yamanaka; Mitsuhiko Sugimoto; Akitsu Hotta
PLoS ONE, Aug. 2014, Peer-reviewed, Last author, Corresponding author

Direct Comparison of Autologous and Allogeneic Transplantation of iPSC-Derived Neural Cells in the Brain of a Nonhuman Primate
Asuka Morizane; Daisuke Doi; Tetsuhiro Kikuchi; Keisuke Okita; Akitsu Hotta; Toshiyuki Kawasaki; Takuya Hayashi; Hirotaka Onoe; Takashi Shiina; Shinya Yamanaka; Jun Takahashi
Stem cell reports, Oct. 2013, Peer-reviewed

Cartilage tissue engineering identifies abnormal human induced pluripotent stem cells
Akihiro Yamashita; Shiying Liu; Knut Woltjen; Bradley Thomas; Guoliang Meng; Akitsu Hotta; Kazutoshi Takahashi; James Ellis; Shinya Yamanaka; Derrick E. Rancourt
Scientific reports, Jun. 2013, Peer-reviewed

Efficient and Reproducible Myogenic Differentiation from Human iPS Cells: Prospects for Modeling Miyoshi Myopathy In Vitro
Akihito Tanaka; Knut Woltjen; Katsuya Miyake; Akitsu Hotta; Makoto Ikeya; Takuya Yamamoto; Tokiko Nishino; Emi Shoji; Atsuko Sehara-Fujisawa; Yasuko Manabe; Nobuharu Fujii; Kazunori Hanaoka; Takumi Era; Satoshi Yamashita; Ken-ichi Isobe; En Kimura; Hidetoshi Sakurai
PLoS ONE, Apr. 2013, Peer-reviewed
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Isolation of MECP2-null Rett Syndrome patient hiPS cells and isogenic controls through X-chromosome inactivation
Aaron Y. L. Cheung; Lindsay M. Horvath; Daria Grafodatskaya; Peter Pasceri; Rosanna Weksberg; Akitsu Hotta; Laura Carrel; James Ellis
Human molecular genetics, Jun. 2011, Peer-reviewed

Constitutive heterochromatin reorganization during somatic cell reprogramming
Eden Fussner; Ugljesa Djuric; Mike Strauss; Akitsu Hotta; Carolina Perez-Iratxeta; Fredrik Lanner; F. Jeffrey Dilworth; James Ellis; David P. Bazett-Jones
EMBO journal, May 2011, Peer-reviewed

Ataxia-telangiectasia mutated (ATM) deficiency decreases reprogramming efficiency and leads to genomic instability in iPS cells
Taisuke Kinoshita; Go Nagamatsu; Takeo Kosaka; Keiyo Takubo; Akitsu Hotta; James Ellis; Toshio Suda
Biochemical and Biophysical Research Communications, Apr. 2011, Peer-reviewed

Stage-Specific Optimization of Activin/Nodal and BMP Signaling Promotes Cardiac Differentiation of Mouse and Human Pluripotent Stem Cell Lines
Steven J. Kattman; Alec D. Witty; Mark Gagliardi; Nicole C. Dubois; Maryam Niapour; Akitsu Hotta; James Ellis; Gordon Keller
Cell Stem Cell, Feb. 2011, Peer-reviewed

MECP2 Isoform-Specific Vectors with Regulated Expression for Rett Syndrome Gene Therapy
Mojgan Rastegar; Akitsu Hotta; Peter Pasceri; Maisam Makarem; Aaron Y. L. Cheung; Shauna Elliott; Katya J. Park; Megumi Adachi; Frederick S. Jones; Ian D. Clarke; Peter Dirks; James Ellis
PLoS ONE, Aug. 2009, Peer-reviewed

Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency
Akitsu Hotta; Aaron Y. L. Cheung; Natalie Farra; Kausalia Vijayaragavan; Cheryle A. Seguin; Jonathan S. Draper; Peter Pasceri; Irina A. Maksakova; Dixie L. Mager; Janet Rossant; Mickie Bhatia; James Ellis
Nature Methods, May 2009, Peer-reviewed, Lead author

EOS lentiviral vector selection system for human induced pluripotent stem cells
Akitsu Hotta; Aaron Y. L. Cheung; Natalie Farra; Kamal Garcha; Wing Y. Chang; Peter Pasceri; William L. Stanford; James Ellis
Nature Protocols, 2009, Peer-reviewed, Invited, Lead author

Retroviral Vector Silencing During iPS Cell Induction: An Epigenetic Beacon that Signals Distinct Pluripotent States
Akitsu Hotta; James Ellis
Journal of cellular biochemistry, Nov. 2008, Peer-reviewed, Invited, Lead author

beta-globin LCR and intron elements cooperate and direct spatial reorganization for gene therapy
Alla Buzina; Mandy Y. M. Lo; Angela Moffett; Akitsu Hotta; Eden Fussner; Rikki R. Bharadwaj; Peter Pasceri; J. Victor Garcia-Martinez; David P. Bazett-Jones; James Ellis
PLOS GENETICS, Apr. 2008, Peer-reviewed

YY1 binds to regulatory element of chicken lysozyme and ovalbumin promoters
Mahboob Morshed; Munetoshi Ando; Junko Yamamoto; Akitsu Hotta; Hidenori Kaneoka; Jun Kojima; Ken-ichi Nishijima; Masamichi Kamihira; Shinji Iijima
Cytotechnology, Nov. 2006, Peer-reviewed

Characterization of transient expression system for retroviral vector production
Akitsu Hotta; Yoshikazu Saito; Kenji Kyogoku; Yoshinori Kawabe; Ken-ichi Nishijima; Masamichi Kamihira; Shinji Iijima
Journal of Bioscience and Bioengineering, Apr. 2006, Peer-reviewed, Lead author

Production of anti-CD2 chimeric antibody by recombinant animal cells
Akitsu Hotta; Masamichi Kamihira; Kanako Itoh; Mahboob Morshed; Yoshinori Kawabe; Ken Ichiro Ono; Hiroyuki Matsumoto; Ken Ichi Nishijima; Shinji Iijima
Journal of Bioscience and Bioengineering, Oct. 2004, Peer-reviewed, Lead author

Production of anti-prion scFv-Fc fusion proteins by recombinant animal cells
KI Ono; M Kamihira; Y Kuga; H Matsumoto; A Hotta; T Itoh; KI Nishijima; N Nakamura; H Matsuda; S Iijima
Journal of Bioscience and Bioengineering, Mar. 2003, Peer-reviewed

Delivery of CRISPR-Cas tools for in vivo genome editing therapy: Trends and challenges.
Eman A Taha; Joseph Lee; Akitsu Hotta
Journal of controlled release : official journal of the Controlled Release Society, Feb. 2022, Peer-reviewed, Invited, Last author, Corresponding author
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Optimized electroporation of CRISPR-Cas9/gRNA ribonucleoprotein complex for selection-free homologous recombination in human pluripotent stem cells.
Huaigeng Xu; Yuto Kita; Uikyu Bang; Peter Gee; Akitsu Hotta
STAR protocols, 17 Dec. 2021, Last author, Corresponding author
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Low immunogenicity of LNP allows repeated administrations of CRISPR-Cas9 mRNA into skeletal muscle in mice.
Eriya Kenjo; Hiroyuki Hozumi; Yukimasa Makita; Kumiko A Iwabuchi; Naoko Fujimoto; Satoru Matsumoto; Maya Kimura; Yuichiro Amano; Masataka Ifuku; Youichi Naoe; Naoto Inukai; Akitsu Hotta
Nature communications, 08 Dec. 2021, Peer-reviewed, Last author, Corresponding author
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Dual inhibition of TMPRSS2 and Cathepsin Bprevents SARS-CoV-2 infection in iPS cells.
Rina Hashimoto; Ayaka Sakamoto; Sayaka Deguchi; Renxing Yi; Emi Sano; Akitsu Hotta; Kazutoshi Takahashi; Shinya Yamanaka; Kazuo Takayama
Molecular therapy. Nucleic acids, 03 Dec. 2021
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Contractile Activity of Myotubes Derived from Human Induced Pluripotent Stem Cells: A Model of Duchenne Muscular Dystrophy.
Kantaro Yoshioka; Akira Ito; Masanobu Horie; Kazushi Ikeda; Sho Kataoka; Keiichiro Sato; Taichi Yoshigai; Hidetoshi Sakurai; Akitsu Hotta; Yoshinori Kawabe; Masamichi Kamihira
Cells, 27 Sep. 2021

Misc.

マウスEWS/FLI1誘導性骨肉腫モデルが明らかとした肉腫発生に関わる重要事項-EWS/FLI1とcancer genomeがもたらす細胞分化異常-
河村真吾; 河村真吾; 蝉克憲; 板倉史晃; 柴田博史; 堀田秋津; WOLTJEN Knut; 山本拓也; 大野貴敏; 秋山治彦; 山田泰広
日本整形外科学会雑誌, 2016

マウスEWS/FLI1誘導性骨肉腫モデルが明らかとした肉腫発生に関わる重要事項-EWS/FLI1とcancer genomeがもたらす細胞分化異常-
河村真吾; 河村真吾; 蝉克憲; 板倉史晃; 柴田博史; 堀田秋津; WOLTJEN Knut; 山本拓也; 大野貴敏; 秋山治彦; 山田泰広
日本整形外科学会雑誌, 2016

EWS-FLI1誘導性骨肉腫モデルによって骨分化障害が肉腫発生に重要な役割をもつことが明らかとなった
河村真吾; 蝉克憲; 板倉史晃; 柴田博史; 堀田秋津; KNUT Woltjen; 山本拓也; 大野貴敏; 秋山治彦; 山田泰広
日本整形外科学会雑誌, 2016

【動き始めたゲノム編集の医療応用免疫再生医療からダウン症治療、異種移植までin vivo/ex vivoでの多彩なアプローチ】(Part I.)いまそこにある課題とその解決法ゲノム編集医療の真の実現化をめざして in vivoゲノム編集の鍵を握るデリバリー技術
パン・イギュ; 小島佑介; 堀田秋津
実験医学, Apr. 2024

【心不全の最新治療-再生医療を用いた最新の知見】HLA遺伝子ノックアウトによるiPS細胞の免疫寛容法の確立
堀田秋津
カレントテラピー, Jan. 2024

【真の実臨床応用をめざした再生医療2023 リバーストランスレーショナルリサーチのいまと産業化のための技術開発】(第5章)ブレイクスルーとなる基礎的知見と課題免疫拒絶を回避するための次世代技術
堀田秋津
実験医学, Feb. 2023

iPS細胞を用いた再生医療のこれから CRISPR-Casゲノム編集ツールによるiPS細胞でのHLA改変と再生医療応用
堀田秋津
MHC: Major Histocompatibility Complex, Sep. 2021

iPS細胞を用いた革新的医療研究ゲノム編集が加速するiPS細胞の治療応用
堀田秋津
組織培養研究, Aug. 2021

【新規の創薬モダリティ細胞医薬細胞を薬として使う、新たな時代の基礎研究と治療法開発】第3章細胞療法の新しい戦略幹細胞ベース免疫汎用性向上をめざしたHLA編集iPS細胞
堀田秋津
実験医学, Nov. 2020

ゲノム編集による低免疫原性iPS細胞の開発
堀田秋津
再生医療, Nov. 2020

筋疾患研究の最前線デュシェンヌ型筋ジストロフィーに対するゲノム編集治療開発への挑戦
堀田秋津; Gee Peter; Lung Mandy; 奥嵜雄也; 笹川典子
脳と発達, Aug. 2020

再生医療はどこまで進んだか(vol.8) iPS細胞臨床応用をめざしたゲノム編集による低抗原性iPS細胞の作製
徐淮耕; 堀田秋津
医学のあゆみ, Jul. 2020

【ゲノム編集を用いた遺伝子治療】テーラーメイド医療を目指したゲノム編集iPS細胞
徐淮耕; 堀田秋津
Precision Medicine, Jun. 2020

用語解説ユニバーサル細胞
堀田秋津
再生医療, May 2020

【遺伝性疾患治療の最前線】ゲノム編集で挑む新時代の遺伝子治療研究
渡邉啓; 北悠人; 奥嵜雄也; 堀田秋津
遺伝子医学, Apr. 2020

ゲノム編集とiPS細胞を用いた難病治療法開発
堀田秋津
金沢医科大学雑誌, Sep. 2019

Duchenne型筋ジストロフィー患者にゲノム編集治療を送達するための技術開発研究
堀田秋津
日本新生児成育医学会雑誌, Oct. 2018

人工RNAを基盤としたCRISPR/Cas9Systemの構築
弘澤萌; 弘澤萌; 樫田俊一; 中村正裕; LI Hongmei; 堀田秋津; 渡辺亮; 齊藤博英
日本RNA学会年会要旨集, 2015

1H14-5 Efficient production of retrovirus vector by transient expressing system.
HOTTA Akitsu; SAITO Yoshikazu; KAMIHIRA Masamichi; NISHIJIMA Ken-ichi; IIJIMA Shinji
日本生物工学会大会講演要旨集, 2005

2F09-4 Development of transient expression system for high-titer retrovirus vector preparation
SAITO Yoshikazu; HOTTA Akitsu; NISHIJIMA Ken-ichi; KAMIHIRA Masamichi; IIJIMA Shinji
日本生物工学会大会講演要旨集, 2004

2F09-5 Preparation of lentivirus vectors for generation of transgenic chickens
OKAJIMA Hiromi; HOTTA Akitsu; NISHIJIMA Ken-ichi; KAMIHIRA Masamichi; IIJIMA Shinji
日本生物工学会大会講演要旨集, 2004

【ゲノム編集を用いた遺伝子治療】テーラーメイド医療を目指したゲノム編集iPS細胞
徐淮耕; 堀田秋津
Precision Medicine, Jun. 2020

用語解説ユニバーサル細胞
堀田秋津
再生医療, May 2020

【遺伝性疾患治療の最前線】ゲノム編集で挑む新時代の遺伝子治療研究
渡邉啓; 北悠人; 奥嵜雄也; 堀田秋津
遺伝子医学, Apr. 2020

【よくわかる遺伝子治療-実用化の現状と展望】遺伝子治療総論ゲノム編集技術の発展とその神経・筋疾患への応用
北悠人; 岩渕久美子; 堀田秋津
Clinical Neuroscience, Mar. 2020

Abstract 3585: T cell receptor-stabilized regenerated CD8ab cytotoxic T lymphocytes for cancer immunotherapy
Atsutaka Minagawa; Akitsu Hotta; Yohei Kawai; Yutaka Yasui; Yasushi Uemura; Masaki Yasukawa; Tetsuya Nakatsura; Shin Kaneko
Immunology, 01 Jul. 2018

【ゲノム編集技術の基礎と応用】ゲノム編集の非ウイルス性送達法および臨床応用戦略
奥嵜雄也; 堀田秋津
血液フロンティア, Jun. 2018

【ゲノム編集技術の基礎と応用】ゲノム編集の非ウイルス性送達法および臨床応用戦略
奥嵜雄也; 堀田秋津
血液フロンティア, Jun. 2018, Invited

【超高齢社会に挑む骨格筋のメディカルサイエンス筋疾患から代謝・全身性制御へと広がる筋研究を、健康寿命の延伸につなげる】 (第5章)骨格筋疾患研究の最前線・展望ゲノム編集技術を利用した筋ジストロフィー研究および治療戦略
鍵田明宏; 徐淮耕; 堀田秋津
実験医学, May 2018

【疾患特異的iPS細胞を用いた病態研究と創薬】ゲノム編集技術とその応用の現状
岩渕久美子; 堀田秋津
血液フロンティア, Feb. 2018

ヒトiPS細胞由来間葉系間質細胞を用いたウルリッヒ型筋ジストロフィー症に対する細胞移植治療研究
竹中菜々; KIM Jinsol; 池谷真; 堀田秋津; 上住聡芳; 佐藤貴彦; 野口悟; 櫻井英俊
日本再生医療学会総会(Web), 2018

Restoration of Dystrophin Protein Expression by Exon Skipping Utilizing CRISPR-Cas9 in Myoblasts Derived from DMD Patient iPS Cells.
Ifuku M; Iwabuchi KA; Tanaka M; Lung MSY; Hotta A
Methods in Molecular Biology, 2018, Invited, Last author, Corresponding author

ゲノム編集技術の現状と展望ヒトiPS細胞での効率的なゲノム編集法および筋ジストロフィーゲノム編集治療応用
堀田秋津
生命科学系学会合同年次大会, Dec. 2017

【iPSの社会的意義】 iPS細胞の遺伝子変異をゲノム編集で修復する疾患研究と遺伝子治療の最先端
堀田秋津
遺伝: 生物の科学, Sep. 2017

iPS細胞とゲノム編集 : 筋ジストロフィー治療の場合 (総特集 iPS細胞の未来 : 山中伸弥の仕事)
堀田秋津
現代思想, Jun. 2017

【ゲノム情報と遺伝子治療-遺伝子治療の最新動向-】ゲノム編集技術の現状と課題 iPS細胞とゲノム編集の融合による新展開
井福正隆; 堀田秋津
日本臨床, May 2017

iPS細胞を用いた筋ジストロフィーに対する細胞移植治療法の開発
櫻井英俊; 高山了; 竹中菜々; 竹中菜々; 趙明明; 田積充年; 田積充年; 池谷真; 堀田秋津; 伊東佑太; 佐藤貴彦
再生医療, 01 Feb. 2017

【筋肉研究の最前線】筋ジストロフィー治療応用を目指したゲノム編集技術の開発動向
蒔田幸正; 穂積裕幸; 堀田秋津
Clinical Calcium, Feb. 2017

NK細胞から見たHLA欠失血小板の有用性と安全性の保証
鈴木大助; 杉本直志; 吉川典秀; 中村壮; 遠藤大; 堀田秋津; 江藤浩之
再生医療, 2017

Cellular Reprogramming, Genome Editing, and Alternative CRISPR Cas9 Technologies for Precise Gene Therapy of Duchenne Muscular Dystrophy
Peter Gee; Huaigeng Xu; Akitsu Hotta
Stem Cells International, 2017, Invited, Last author, Corresponding author

Natural Killer Cell Activities Against iPSCs-Derived HLA-Knockout Platelets and Megakaryocytes Reveal Perfect Rejection Profiles for Allotransfusion
Daisuke Suzuki; Naoshi Sugimoto; Norihide Yoshikawa; Hiroshi Endo; Sou Nakamura; Akitsu Hotta; Koji Eto
Blood, 02 Dec. 2016

【All Aboutゲノム編集"革命的技術"はいかにして私たちの研究・医療・産業を変えるのか?】 (第2章)生命科学・疾患治療研究への最新導入例ヒトでのゲノム編集遺伝子治療応用へと動き出した現状
石田賢太郎; 徐淮耕; 堀田秋津
実験医学, Dec. 2016

【筋ジストロフィー・筋疾患-最近の進歩】ミオパチーの臨床と研究の最新トピックス Duchenne型筋ジストロフィーに対するゲノム編集戦略
徐淮耕; 堀田秋津
医学のあゆみ, Oct. 2016

Efficient genomic correction methods in human iPS cells using CRISPR–Cas9 system
Hongmei Lisa Li; Peter Gee; Kentaro Ishida; Akitsu Hotta
Methods, May 2016, Invited, Last author, Corresponding author

家族性OPLL患者由来のiPS細胞を用いたOPLL遺伝的要素の検討
川井俊介; 羽田匡孝; 小山優子; 池谷真; ALEV Cantas; 堀田秋津; 池川志郎; 中村雅也; 松本守雄; 吉富啓之; 松田秀一; 戸口田淳也
日本整形外科学会雑誌, 2016

iPS細胞技術に基づくHLA欠失血小板の作製と実用可能性の検証
鈴木大助; 杉本直志; 吉川典秀; 中村壮; 遠藤大; 堀田秋津; 江藤浩之
再生医療, 2016

Novel Gene Therapy Strategy for Hemophilia a By Hydrodynamic Gene Delivery Combined with Non-Viral Piggybac Transposon Vector in Canine Model
Masashi Noda; Hideto Matsui; Yasunori Matsunari; Sunao Ochi; Midori Shima; Yasushi Fukuoka; Takeshi Sato; Hiroshi Anai; Kimihiko Kichikawa; Masanari Nakayama; Akitsu Hotta; Mitsuhiko Sugimoto
Blood, 03 Dec. 2015

DNA修復経路の制御によるCRISPR-Cas9を用いたゲノム編集効率化の検討
石田賢太郎; 李紅梅; 堀田秋津
日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, Dec. 2015

心臓運命をプログラムする因子とその発展性
森田唯加; 堀田秋津; Andersen Peter; 小川英知; 吉田善紀; 塚原由布子; 黒川洵子; 相賀裕美子; Evans Sylvia; 西中村隆一; 小柴和子; Kwon Chulan; 竹内純
日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, Dec. 2015

iPS細胞を用いた筋ジストロフィーに対する細胞移植治療法の開発
櫻井英俊; 趙明明; 高山了; 竹中菜々; 田積充年; 佐藤優江; 池谷真; 堀田秋津; 佐藤貴彦; 伊東佑太; 関口清俊
日本再生医療学会総会(Web), Dec. 2015

Minimizing off-Target Mutagenesis Risks Caused by Programmable Nucleases
Kentaro Ishida; Peter Gee; Akitsu Hotta
International Journal of Molecular Sciences, 16 Oct. 2015, Peer-reviewed, Invited, Last author, Corresponding author
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Genome Editing Gene Therapy for Duchenne Muscular Dystrophy
Akitsu Hotta
Journal of Neuromuscular Diseases, 22 Sep. 2015, Peer-reviewed, Invited, Lead author, Last author, Corresponding author

iPS細胞を用いた神経疾患の最新研究デュシェンヌ型筋ジストロフィーiPS細胞を用いたゲノム編集遺伝子治療
堀田秋津
神経治療学, Sep. 2015

アクチンは細胞のリプログラミングを制御する
池田隆; 引地貴亮; 渡辺亮; 堀田秋津; 升井伸治
日本細胞生物学会大会講演要旨集, Jun. 2015

【再生医療-新たな医療を求めて-】臨床応用を目指した基礎研究再生医療を目指した前臨床研究心機能再生を目指した特定因子による細胞変換技術開発
森田唯加; 藤川大地; 堀田秋津; 吉田善紀; 小柴和子; 竹内純
日本臨床, Jun. 2015

【病理医を誘うiPS細胞を使った疾患研究】疾患iPS細胞研究を加速する遺伝子改変技術の進歩
堀田秋津; 石田賢太郎; 佐伯涼太
病理と臨床, Jun. 2015

Genomic correction of mutant TDP-43 iPSCs
Tsuge I; Tsukita K; Naito M; Suzuki S; Sakuma T; Yamamoto T; Hotta A; Yamanaka S; Inoue H
56th Annual Meeting of the Japanese Society of Neurology, Niigata., 21 May 2015

571. Precise Control of CRISPR-Cas9 Mediated Gene Editing for Correcting Mutation of Duchenne Muscular Dystrophy in iPS Cells
Hongmei L. Li; Norko Sasakawa; Kentaro Ishida; Peter Gee; Akitsu Hotta
Molecular Therapy, May 2015

【再生医療2015 幹細胞と疾患iPS細胞の研究最前線】 (第5章)幹細胞技術開発再生医療に向けたゲノム編集技術
李紅梅; 佐伯涼太; 堀田秋津
実験医学, Feb. 2015

iPS細胞研究最前線疾患モデルから臓器再生まで(Vol.14) 患者由来iPS細胞を用いた神経疾患研究
津下到; 鈴木茂彦; 内藤素子; 堀田秋津; 井上治久
医学のあゆみ, Feb. 2015

Editing cultured human cells: From cell lines to iPS cells
Hongmei Lisa Li; Akitsu Hotta
Targeted Genome Editing Using Site-Specific Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System, 01 Jan. 2015, Invited

Induced pluripotent stem cells
Akitsu Hotta; Shinya Yamanaka
Biomaterials and Regenerative Medicine, 01 Jan. 2015, Invited

【ゲノム編集-基礎から応用へ】 iPS細胞応用を加速するゲノム編集技術
堀田秋津
医学のあゆみ, Jan. 2015

From Genomics to Gene Therapy: Induced Pluripotent Stem Cells Meet Genome Editing
Akitsu Hotta; Shinya Yamanaka
Annual review of genetics, 2015, Peer-reviewed, Invited, Lead author

ヒトiPS細胞由来のアストロサイトを用いたALSモデルマウスの脊髄移植治療
近藤孝之; 舟山美里; 月田香代子; 堀田秋津; 安田明正; 海苔聡; 金子慎二郎; 中村雅也; 高橋良輔; 岡野栄之; 山中伸弥; 井上治久
55th Annual Meeting of the Japanese Society of Neurology, Fukuoka., Dec. 2014

ゲノム編集iPS細胞を利用した抗腫瘍細胞免疫療法の開発(Development of iPSC based adoptive immunotherapy by using genome editing strategy)
南川淳隆; 堀田秋津; 川名敬; 金子新
日本癌学会総会記事, Sep. 2014

【ゲノム編集法の新常識!CRISPR/Casが生命科学を加速する】 CRISPR/Casを用いた遺伝子治療への展望
李紅梅; 堀田秋津
実験医学, Jul. 2014

322. Engineered Nuclease Mediated Genetic Correction in iPSCs Derived From Duchenne Muscular Dystrophy Patient
Molecular Therapy, May 2014

【直接リプログラミング研究の最前線】細胞種変換を支える遺伝子導入技術
堀田秋津
細胞, May 2014

細胞治療血友病Aに対する新規遺伝子/細胞治療戦略トランスポゾンベクターによる遺伝子治療
堀田秋津
日本血栓止血学会誌, Apr. 2014

Genetic correction using engineered nucleases for gene therapy applications
Hongmei Lisa Li; Takao Nakano; Akitsu Hotta
Development Growth & Differentiation, Jan. 2014, Peer-reviewed, Invited, Last author, Corresponding author

Autologous Transplantation of Induced Pluripotent Stem Cell-Derived Neurons Causes Minimum Immune Reaction in the Brain of Non-Human Primate
Morizane, A; Doi, D; Kikuchi, T; Okita, K; Hotta, A; Kawasaki, T; Onoe, H; Shiina, T; Takahashi, J
ISSCR 11th Annual Meeting, Jun. 2013, Peer-reviewed

357. Long-Term Phenotypic Correction of Hemophilia A by Non-Viral PiggyBac Transposon Vector
Hideto Matsui; Mitsuhiko Sugimoto; Midori Shima; Naoko Fujimoto; Akitsu Hotta
Molecular Therapy, May 2013, Last author, Corresponding author

【ゲノム編集革命:遺伝子改変はZFN・TALEN・CRISPR/Cas三強時代へ】 TALENを用いたヒトiPS細胞におけるゲノム編集
李紅梅; Woltjen Knut; 高橋和利; 山中伸弥; 堀田秋津
細胞工学, Apr. 2013

血友病の新規iPS細胞遺伝子治療戦略
堀田秋津
上原記念生命科学財団研究報告集, Dec. 2012

イヌ体細胞由来人工多能性幹細胞の樹立
早川和男; 加藤友久; 那須輝; 池谷真; 堀田秋津; 大塚隆信; 戸口田淳也
日本整形外科学会雑誌, Aug. 2012

iPS細胞の小分子制御
堀田秋津
学術の動向, 01 May 2011, Invited

Disruption of constitutive heterochromatin is a biomarker of pluripotency
Eden Fussner; Ugljesa Djuric; Akitsu Hotta; Mike Strauss; James Ellis; David P. Bazett-Jones
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, Feb. 2011

Obtaining Pluripotency Biomarker Signatures
Kathryn Sciabica; Knut Woltjen; Akitsu Hotta
Genetic Engineering & Biotechnology News., Feb. 2010

56. EOS Lentivector Selection of Mouse and Human Rett Syndrome iPS Cells
James Ellis; Natalie Farra; Aaron Cheung; Peter Pasceri; Ugljesa Djuric; Akitsu Hotta
Molecular Therapy, May 2009, Last author

Retrovirus silencing by an epigenetic TRIM
James Ellis; Akitsu Hotta; Mojgan Rastegar
Cell, Oct. 2007, Invited

Presentations

ヘルマンスキー・パドラック症候群2型患者由来iPS細胞を用いて2型肺胞上皮細胞の機能異常を再現する
興梠陽平; 後藤慎平; 池尾聡; 山本佑樹; 曽根尚之; 玉井浩二; 浅香勲; 堀田秋津; 平井豊博
日本肺サーファクタント・界面医学会雑誌, Oct. 2019, 日本肺サーファクタント・界面医学会

ゲノム編集とiPS細胞を用いた難病治療法開発
堀田秋津
金沢医科大学雑誌, Sep. 2019, 金沢医科大学医学会

ウイルス様粒子送達システムを用いた筋ジストロフィーのゲノム編集治療法開発研究
Gee Peter; 笹川典子; Lung Mandy; 奥嵜雄也; 鍵田明宏; 北悠人; 渡邉啓; 岩渕久美子; 堀田秋津
日本筋学会学術集会プログラム・抄録集, Aug. 2019, 日本筋学会

ヒトiPS細胞由来肺胞オルガノドを用いたHermansky-Pudlak症候群2型の疾患モデリング
興梠陽平; 後藤慎平; 池尾聡; 山本佑樹; 曽根尚之; 玉井浩二; 小西聡史; 長崎忠雄; 松本久子; 伊藤功朗; 陳豊史; 伊達洋至; 萩原正敏; 浅香勲; 堀田秋津; 三嶋理晃; 平井豊博
組織培養研究, Jun. 2019, 日本組織培養学会

ゲノム編集が加速するiPS細胞研究と筋ジストロフィー治療への挑戦
堀田秋津
日本医学会総会会誌, Apr. 2019, 日本医学会

Duchenne型筋ジストロフィー患者にゲノム編集治療を送達するための技術開発研究
堀田秋津
日本新生児成育医学会雑誌, Oct. 2018, (公社)日本新生児成育医学会

HLAクラスI欠失ヒトiPS細胞由来血小板の抗HLA抗体およびNK細胞に対する免疫逃避(HLA class-I depleted human iPSC-derived platelets evade anti-HLA and NK cell immunity)
杉本直志; 鈴木大助; シャオミン・フェング; 吉川典秀; 赤坂まりな; 中村壮; 遠藤大; 堀田秋津; 滝澤仁; 江藤浩之
臨床血液, Sep. 2018, (一社)日本血液学会-東京事務局

ヒトiPS細胞由来骨格筋幹細胞を用いた筋ジストロフィーに対する細胞移植治療
櫻井英俊; 田積充年; 田積充年; 高山了; 高山了; 竹中菜々; 趙明明; 佐藤優江; 池谷真; 堀田秋津; 佐藤貴彦; 伊東佑太; 関口清俊
日本筋学会学術集会プログラム・抄録集, Aug. 2018, 日本筋学会

患者由来iPS細胞モデルで明らかにした顔面肩甲上腕型筋ジストロフィーの病態における酸化ストレスの役割
本田充; 城之内達也; 新井芽丹; 堀田秋津; 三橋里美; 西野一三; 松田良一; 櫻井英俊
日本筋学会学術集会プログラム・抄録集, Aug. 2018, 日本筋学会

CRISPR-Cas9を用いたディスフェルリノパチー患者由来iPS細胞におけるDYSF遺伝子の高効率一塩基修復法
鍵田明宏; 徐淮耕; ジー・ピーター; 永井未来; 櫻井英俊; 堀田秋津
日本筋学会学術集会プログラム・抄録集, Aug. 2018, 日本筋学会

iPSC技術を用いた養子T細胞療法のための普遍的なドナー細胞供給源の作製(Generation of universal donor cell source for adoptive T cell therapy using iPSC technology)
王博; 入口翔一; 徐淮耕; 上田樹; 上田格弘; 南川淳隆; 喜多山秀一; 植村靖史; 中面哲也; 堀田秋津; 金子新
日本がん免疫学会総会プログラム・抄録集, Jul. 2018, 日本がん免疫学会

ゲノム編集技術の現状と展望ヒトiPS細胞での効率的なゲノム編集法および筋ジストロフィーゲノム編集治療応用
堀田秋津
生命科学系学会合同年次大会, Dec. 2017, 生命科学系学会合同年次大会運営事務局

網羅的ゲノム編集による細胞外シグナル起因性の細胞老化の制御
沖田喜樹; 渡辺亮; 堀田秋津; 升井伸治
生命科学系学会合同年次大会, Dec. 2017, 生命科学系学会合同年次大会運営事務局

顔面肩甲上腕型筋ジストロフィにおける外的ストレスの病態関与の検討
本田充; 堀田秋津; 三橋里美; 城之内達也; 西野一三; 松田良一; 櫻井英俊
生命科学系学会合同年次大会, Dec. 2017, 生命科学系学会合同年次大会運営事務局

筋生物学の統合的理解にむけた新たな潮流 iPS細胞からの骨格筋幹細胞誘導と、筋ジストロフィーに対する再生医療への応用
櫻井英俊; 高山了; 田積充年; 竹中菜々[蜷川]; 趙明明; 池谷真; 堀田秋津; 佐藤貴彦; 伊東佑太
生命科学系学会合同年次大会, Dec. 2017, 生命科学系学会合同年次大会運営事務局

iPS細胞技術を用いたT細胞治療療法のためのユニバーサルドナー細胞の作製
王博; 入口翔一; 上田樹; 上田格弘; 南川淳隆; 喜多山秀一; 植村靖史; 中面哲也; 江藤浩之; 堀田秋津; 金子新
日本癌学会総会記事, Sep. 2017, 日本癌学会

患者由来iPS細胞を用いた顔面肩甲上腕型筋ジストロフィにおける環境因子の病態関与の検討
本田充; 堀田秋津; 三橋里美; 城之内達也; 西野一三; 松田良一; 櫻井英俊
日本筋学会学術集会プログラム・抄録集, Jul. 2017, 日本筋学会

Natural Killer Cell Activities Against iPSCs-Derived HLA-Knockout Platelets and Megakaryocytes Reveal Perfect Rejection Profiles for Allotransfusion
Daisuke Suzuki; Naoshi Sugimoto; Norihide Yoshikawa; Hiroshi Endo; Sou Nakamura; Akitsu Hotta; Koji Eto
BLOOD, Dec. 2016, AMER SOC HEMATOLOGY

ゲノム編集技術を用いた生化学の新展望 CRISPRツールボックスによるヒトゲノム編集効率化
堀田秋津
日本生化学会大会プログラム・講演要旨集, Sep. 2016, (公社)日本生化学会

家族性OPLL患者由来のiPS細胞を用いたOPLL遺伝的要素の検討
川井俊介; 羽田匡孝; 小山優子; 池谷真; Alev Cantas; 堀田秋津; 池川志郎; 中村雅也; 松本守雄; 吉富啓之; 松田秀一; 戸口田淳也
日本整形外科学会雑誌, Aug. 2016, (公社)日本整形外科学会

遺伝子改変技術によるセルエンジニアリングの革新産業応用に向けて iPS細胞での効率的なゲノム編集を目指したCRISPRツールボックス
堀田秋津
日本生物工学会大会講演要旨集, Aug. 2016, (公社)日本生物工学会

EWS-FLI1誘導性骨肉腫モデルによって骨分化障害が肉腫発生に重要な役割をもつことが明らかとなった
河村真吾; 蝉克憲; 板倉史晃; 柴田博史; 堀田秋津; Woltjen Knut; 山本拓也; 大野貴敏; 秋山治彦; 山田泰広
日本整形外科学会雑誌, Aug. 2016, (公社)日本整形外科学会

Non-viral piggyBac transposon vector based gene therapy for hemophilia A
Hideto Matsui; Masashi Noda; Yasunori Matsunari; Sunao Ochi; Midori Shima; Yasushi Fukuoka; Takeshi Sato; Kimihiko Kichikawa; Akitsu Hotta; Mitsuhiko Sugimoto
HAEMOPHILIA, Jul. 2016, WILEY-BLACKWELL

訪問リハビリテーションにおけるリハ医の関与リハビリテーションマネージメント加算(II)に関連して
奥村元昭; 眞島利匡; 水本保子; 堀田修秀
The Japanese Journal of Rehabilitation Medicine, Jun. 2016, (公社)日本リハビリテーション医学会

マウスEWS/FLI1誘導性骨肉腫モデルが明らかとした肉腫発生に関わる重要事項 EWS/FLI1とcancer genomeがもたらす細胞分化異常
河村真吾; 蝉克憲; 板倉史晃; 柴田博史; 堀田秋津; Woltjen Knut; 山本拓也; 大野貴敏; 秋山治彦; 山田泰広
日本整形外科学会雑誌, Jun. 2016, (公社)日本整形外科学会

ハイドロダイナミックジーンデリバリーシステムによる新規血友病遺伝子治療法の確立
野田正志; 松井英人; 松成泰典; 越智すなお; 嶋緑倫; 福岡靖史; 佐藤健司; 吉川公彦; 中山正成; 堀田秋津; 杉本充彦
日本血栓止血学会誌, May 2016, (一社)日本血栓止血学会

血友病の克服を目指して iPS細胞と遺伝子工学を用いた血友病A遺伝子治療を目指して
堀田秋津
日本血栓止血学会誌, May 2016, (一社)日本血栓止血学会

iPS細胞を用いた神経疾患の最新研究デュシェンヌ型筋ジストロフィーiPS細胞を用いたゲノム編集遺伝子治療
堀田秋津
神経治療学, Mar. 2016, (一社)日本神経治療学会

マウスEWS/FLI1誘導性骨肉腫モデルが明らかとした肉腫発生に関わる重要事項 EWS/FLI1とcancer genomeがもたらす細胞分化異常
河村真吾; 蝉克憲; 板倉史晃; 柴田博史; 堀田秋津; Woltjen Knut; 山本拓也; 大野貴敏; 秋山治彦; 山田泰広
日本整形外科学会雑誌, Mar. 2016, (公社)日本整形外科学会

細胞標識システムを用いた分化におけるヒトES/iPS細胞の不均一性の追跡
佐伯涼太; 仲埜峻雄; 堀田秋津
日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, Dec. 2015, (公社)日本生化学会

Multicolor labeling system for tracking long-term expression and cell-to-cell heterogeneity.
Ryota Saeki; Takao Nakano; Akitsu Hotta
Takeda International Symposium, 2015

Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9
Hongmei Lisa Li; Naoko Fujimoto; Noriko Sasakawa; Shirai Saya; Tokiko Ohkame; Takashi Yamamoto; Akira Watanabe; Hidetoshi Sakurai; Shinya Yamanaka; Akitsu Hotta
Cambridge Healthtech Institute’s Inaugural New Frontiers in Gene Editing Part of the 22nd International Molecular Medicine Tri-Conference, 2015

TALENおよびCRISPRを用いた高効率かつ高精度な筋ジストロフィーiPS細胞の遺伝子修復
堀田秋津
第14回日本再生医療学会総会, 2015

Precise control of CRISPR-Cas9 mediated gene editing for correcting mutation of Duchenne muscular dystrophy in iPS Cells
Hongmei L Li; Norko Sasakawa; Kentaro Ishida; Peter Gee; Akitsu Hotta
American Society of Gene & Cell Therapy (ASGCT) 18th Annual Meeting, 2015

ゲノム編集による効率的な遺伝子変異修復戦略
堀田秋津
ゲノム創薬・医療フォーラム第2回シンポジウム, 2015, Invited

iPS細胞を用いた遺伝子治療戦略:トランスポゾンベクターとゲノム編集
堀田秋津
第15回遺伝子デリバリー研究会, 2015, Invited

ゲノム編集の医療応用への可能性と課題
堀田秋津
国際高等研究所研究プロジェクト, 2015, Invited

疾患iPS細胞における新規遺伝子修復戦略
堀田秋津
第39回阿蘇シンポジウム, 2015, Invited

Efficient gene correction of Duchunne muscular dystrophy mutation in iPS cells by TALENs and CRISPR-Cas9
堀田秋津
第21回日本遺伝子治療学会総会, 2015, Invited

ヒトゲノム配列を自在に書き換えるゲノム編集技術：change mode +w
堀田秋津
NGS現場の会第四回研究会, 2015, Invited

疾患iPS細胞における病原変異遺伝子のゲノム手術
堀田秋津
第６２回日本実験動物学会総会, 2015, Invited

Precise Editing of iPS Cell Genome: Perspectives on DMD Gene Therapy
堀田秋津
第5回新潟大学脳研究所共同研究拠点国際シンポジウム, 2015, Invited

Towards gene therapy using iPS cells: transposon and genome editing technologies
堀田秋津
第７回NAGOYA グローバルリトリート, 2015, Invited

iPS細胞でゲノムを手術してジストロフィン変異を修復する
堀田秋津
筋ジストロフィ−合同班会議市民公開講座, 2015, Invited

Genomic correction of Duchenne Muscular Dystrophy patient-derived iPSCs using engineered nucleases
Hongmei Lisa Li; Naoko Fujimoto; Noriko Sasakawa; Shirai Saya; Takashi Yamamoto; Knut Woltjen; Hidetoshi Sakurai; Shinya Yamanaka; Akitsu Hotta
武田シンポジウム, 2014

Stable and long-term expression of piggyBac vectors by the D4Z4 insulator in ES / iPS cells
Takao Nakano; Sylvie Rival-Gervier; Shinya Yamanaka; Akitsu Hotta
武田シンポジウム, 2014

Engineered nuclease mediated genetic correction in iPSCs derived from Duchenne Muscular Dystrophy patient
Hongmei Lisa Li; Naoko Fujimoto; Noriko Sasakawa; Shirai Saya; Takashi Yamamoto; Knut Woltjen; Hidetoshi Sakurai; Shinya Yamanaka; Akitsu Hotta
ASGCT, 2014

Precise Gene Correction of Duchenne Muscular Dystrophy iPS cells using TALENs and CRISPR/Cas9
Hongmei Lisa Li; Naoko Fujimoto; Noriko Sasakawa; Saya Shirai; Takashi Yamamoto; Knut Woltjen; Akira Watanabe; Hidetoshi Sakurai; Shinya Yamanaka; Akitsu Hotta
ISSCR, 2014

デュシェンヌ型筋ジストロフィー患者由来iPS細胞におけるTALENやCRISPRを用いたジストロフィン遺伝子の修復
李紅梅; 藤本直子; 笹川典子; 白井紗矢; 大亀登紀子; 渡辺亮; 櫻井英俊; 山本卓; 山中伸弥; 堀田秋津
第37回分子生物学会, 2014

ゲノム編集技術を用いたDMD患者由来iPS細胞での遺伝子治療
堀田秋津
筋ジストロフィーモデル動物を用いた新たな治療法の開発平成26年度研究班会議プログラム, 2014, Invited

TALENおよびCRISPR/Cas9を利用した疾患iPS細胞の遺伝子修復
堀田秋津
第87回日本生化学会, 2014, Invited

Duchenne型筋ジストロフィーiPS細胞の遺伝子修復とゲノムワイド変異解析
堀田秋津
第４回ゲノム研究編集研究会, 2014, Invited

ゲノム編集技術を用いたiPS細胞での遺伝子治療
堀田秋津
東大分生研セミナー, 2014, Invited

TALENやCRISPRを用いた疾患iPS細胞のゲノム手術
堀田秋津
第35回日本炎症・再生医学会, 2014, Invited

血友病Aに対する新規遺伝子/細胞治療戦略〜トランスポゾンベクターによる遺伝子治療〜
堀田秋津
第36回日本血栓止血学会学術集会, 2014, Invited

「研究者」という選択〜iPS細胞との出会い〜
堀田秋津
志津南小学校, 2014, Invited

ヒト由来インシュレーターを搭載したトランスポゾンベクターによるES/iPS細胞での長期安定発現
仲埜峻雄; Rival-Gervier Sylvie; 笹川典子; 藤本直子; 白井紗矢; Woltjen Knut; 山中伸弥; 堀田秋津
第１２回日本再生医療学会総会, 2013

Long-Term Phenotypic Correction of Hemophilia A by Non-Viral PiggyBac Transposon Vector
Hideto Matsui; Mitsuhiko Sugimoto; Midori Shima; Naoko Fujimoto; Akitsu Hotta
The ASGCT 16th Annual Meeting, 2013

Genomic correction of Duchenne Muscular Dystrophy patient-derived iPSCs using TALENs
Hongmei Li; Naoko Fujimoto; Noriko Sasakawa; Takashi Yamamoto; Knut Woltjen; Hidetoshi Sakurai; Shinya Yamanaka; Akitsu Hotta
The ASGCT 16th Annual Meeting, 2013

Duchenne型筋ジストロフィー患者由来iPS細胞におけるTALENを用いたゲノム上での遺伝子修復
Hongmei Li; Naoko Fujimoto; Noriko Sasakawa; Takashi Yamamoto; Knut Woltjen; Hidetoshi Sakurai; Shinya Yamanaka; Akitsu Hotta
第三回ゲノム編集研究会, 2013

TALENやCRISPR/Cas9を用いたデュシェンヌ型筋ジストロフィー患者由来iPS細胞のゲノム手術
堀田秋津
第36回日本分子生物学会年会, 2013, Invited

人工ヌクレアーゼ技術を利用したiPS細胞での遺伝子修復
堀田秋津
Meet the Expert 2013, 2013, Invited

ヒトiPS細胞におけるゲノム編集技術を用いた遺伝子異常修復
堀田秋津
NGS現場の会第三回, 2013, Invited

ヒト・マウスゲノムにおけるリピート領域のエピゲノム解析への挑戦
堀田秋津
NGS現場の会第２回研究会, 2012

SITE SPECIFIC GENOME EDITING OF HUMAN INDUCED PLURIPOTENT STEM CELLS BY PNA
Li, Hongmei; Fujimoto, N; Sasakawa, N; Shigi, N; Komiyama, M; Hotta, A; Yamanaka, S
ISSCR 10th annual meeting, 2012

FETAL EPIGENETIC MODIFIERS STIMULATE CARDIOMYOCYTE REGENERATION AND PROTECT FIBROSIS IN MAMMALIAN/AMPHIBIAN MODELS.
Koshiba-Takeuchi, Kazuko; Nakamura, Ryo; Sasano, Tetsuo; Hotta, Akitsu; Tsukahara, Yuko; Yamada, Sawaka; Kojima, Mizuyo; Furukawa, Tetsushi; Olson, Eric; Sadek, Hesham; Takeuchi, Jun K
ISSCR 10th annual meeting, 2012

DERIVATION OF NANOG KNOCK-IN GFP REPORTER IPS CELL LINES USING MEGANUCLEASE-INDUCED HOMOLOGOUS RECOMBINATION
Duhamel, Marianne; Perillous, Sophie; Epinat, Jean-Charles; Cedrone, Fr_d_ric; Englund; Mikael, Ellerstr_m; Catharina, Hyllner; Johan; Hotta, Akitsu; Yamanaka; Shinya, Balbirnie; Edward; Sourdive; David, Rochon; Christelle
ISSCR 10th annual meeting, 2012

GENERATION FUNCTIONAL MYOCYTES FROM HUMAN INDUCED PLURIPOTENT STEM CELLS
Tanaka, Akihito; Woltjen, Knut; Hotta, Akitsu; Ikeya, Makoto; Shoji, Emi; Isobe, Ken-ichi; Sakurai, Hidetoshi
ISSCR 10th annual meeting, 2012

GENERATION OF CANINE IPS CELLS AND THEIR CHARACTERISTICS
Kazuo, Hayakawa; Tomohisa, Kato; Nasu, Akira; Makoto, Ikeya; Takanobu; Otsuka; Akitsu; Hotta, Junya; Toguchida
ISSCR 10th annual meeting, 2012

Establishment of the novel piggyBac transposon vector system for the treatment of hemophilia A
Hideto Matsui; Akitsu Hotta; Naoko Fujimoto; Mitsuhiko Sugimoto; Midori Shima
第74回日本血液学会学術集会, 2012

トランスポゾン発現抑制を司る因子群のshRNAライブラリーによる全ゲノム規模探索
堀田秋津; 仲埜峻雄; 白井紗矢; 笹川典子; 藤本直子; Sylvie Rival-Gervier; 山本卓也; 山中伸弥
第35回日本分子生物学会年会, 2012

Duchenne型筋ジストロフィー患者由来iPS細胞におけるTALENを用いたゲノム上でのエクソンスキッピング
李紅梅; 藤本直子; 笹川典子; 山本卓; ウォルツェンクヌート; 櫻井英俊; 山中伸弥; 堀田秋津
第35回日本分子生物学会年会, 2012

Transposon Technologies Towards iPS Cell Gene Therapy
Akitsu Hotta
理研CDBセミナー, 2012, Invited

Reprogramming the Structure of Constitutive Heterochromatin
Akitsu Hotta
4th iCeMS Retreat, 2012, Invited

CONSTITUTIVE HETEROCHROMATIN REORGANIZATION DURING MOUSE SOMATIC CELL REPROGRAMMING
Djuric; Ugljesa, Fussner; Eden, Hotta; Akitsu; Perez-Iratxeta; Carolina, Lanner; Fredrik, Dilworth; F. Jeffrey; Bazett-Jones; David P; Ellis, James
ISSCR 9th Annual Meeting, 2011

ISOLATION OF MECP2-NULL RETT SYNDROME PATIENT HIPS CELLS AND ISOGENIC CONTROLS THROUGH X-CHROMOSOME INACTIVATION
Cheung, Aaron YL; Horvath; Lindsay M; Grafodatskaya; Daria; Pasceri, Peter; Weksberg, Rosanna; Hotta, Akitsu; Carrel, Laura; Ellis, James
ISSCR 9th Annual Meeting, 2011

REVEALING THE ONCOGENIC RISK OF HUMAN INDUCED PLURIPOTENT STEM CELLS USING IN VITRO CARTILAGE TISSUE ENGINEERING.
Yamashita, Akihiro; Thomas, Bradley; Meng, Guoliang; Hotta, Akitsu; Ellis, James; Rancourt; Derrick E
ISSCR 9th Annual Meeting, 2011

転写因子を用いた効率的なヒトiPS細胞からの骨格筋細胞誘導法の確立
田中章仁; Knut Woltjen; 近藤潤一; 西野永希子; 池谷真; 堀田秋津; 磯部健一; 櫻井英俊
第３４回日本分子生物学会, 2011

iPS細胞が分化万能性を獲得する過程におけるクロマチン高次構造の変化
堀田秋津
第40回北陸実験動物研究会, 2011, Invited

Conversion of partially-reprogrammed iPS cells into a fully-reprogrammed stage induces dynamic changes in heterochromatin ultrastructure.
Akitsu Hotta; Ugljesa Djuric; Eden Fussner; David P. Bazett-Jones; James Ellis
第３３回日本分子生物学会年会、第８３回日本生化学会大会合同大会, 2010

Efficient and stable expression of non-viral vectors in human iPS cells: towards a gene therapy approach for hemophilia.
Akitsu Hotta
ASM 2010, 2010

iPS cell reprogramming and chromatin architecture: How to distinguish high-quality iPS cells?
Akitsu Hotta
Stem Cells and Biomaterials Symposium, 2010, Invited

Efficient and stable expression of non-viral vectors in human iPS cells
Akitsu Hotta
2nd iCeMS retreat, 2010, Invited

Books and Other Publications

現代思想 2017年6月臨時増刊号総特集◎iPS細胞の未来 ―山中伸弥の仕事―
山中伸弥; 池田清彦; 林克彦; 堀田秋津; 美馬達哉; 小泉義之; 塚原東吾; 粥川準二; 八代嘉美, Joint work
青土社, May 2017, Not refereed
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ゲノム編集成功の秘訣Q&A : 論文だけではわからない : TALEN、CRISPR/Cas9の極意
山本卓; 佐久間哲史; 落合博; 李紅梅; 堀田秋津; 藤原祥高; 伊川正人; 真下知士; 鈴木賢一; 川原敦雄; 木下政人; 笹倉靖徳; 林茂生; 大門高明; 杉拓磨; 安本周平; 關光; 刑部祐里子; 刑部敬史; 村中俊哉
羊土社, 2015, Not refereed
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Targeted Genome Editing Using Site-Specific Nucleases
Li, LH; Hotta, A, Chapter 3: Editing Cultured Human Cells: From Cell Lines to iPS Cells
Springer, 2015, Not refereed
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再生医療2015 幹細胞と疾患iPS細胞の研究最前線
李紅梅; 佐伯涼太; 堀田秋津, 第5章幹細胞技術開発「再生医療に向けたゲノム編集技術」
羊土社, 2015, Not refereed
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Biomaterials and Regenerative Medicine
Hotta, A; Yamanaka, S, Chapter 2: Induced Pluripotent Stem Cells
Cambridge University Press, 2014, Not refereed
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ES・iPS細胞実験スタンダード
李紅梅; 佐久間哲史; 堀田秋津; 山本卓, 遺伝子改変法４：TALENによる遺伝子ターゲティング
羊土社, 2014, Not refereed
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Industrial Property Rights

特許第7440027号, 特開2022-101655, 特願2020-089304, 多能性幹細胞から免疫細胞療法用T細胞を誘導する方法
河本宏; 金子新; 増田喬子; 南川淳隆; 堀田秋津; 島津裕; 一瀬大志

特許第7395159号, JP2019048426, ゲノムDNAに欠失を誘導する方法
堀田秋津; 奥嵜雄也; 徐淮耕; ジーピーターデビド; 北悠人; 真下知士; 吉見一人

特許第7385281号, JP2019005524, 低抗原性細胞の製造方法
堀田秋津; 徐淮耕; 金子新; 王博

特開2023-126897, 特願2023-109941, 低抗原性細胞の製造方法
堀田秋津; 徐淮耕; 金子新; 王博

特開2023-120366, 特願2023-101919, ウイルス様粒子及びその使用
堀田秋津; ジーピーターデビド

特許第7306721号, JP2019027708, ウイルス様粒子及びその使用
堀田秋津; ジーピーターデビド

特許第7288282号, JP2018048034, 標的遺伝子改変用組成物
堀田秋津; 井福正隆; 藤本直子; 岩渕久美子; 見城江利也; 蒔田幸正; 落合留美子

特許第7210028号, JP2018026676, 遺伝子変異導入方法
堀田秋津; 石田賢太郎

特開2022-101655, 特願2022-071495, 多能性幹細胞から免疫細胞療法用T細胞を誘導する方法
河本宏; 金子新; 増田喬子; 南川淳隆; 堀田秋津; 島津裕; 一瀬大志

特許第7072808号, 特開2020-141695, 特願2016-534507, 多能性幹細胞から免疫細胞療法用T細胞を誘導する方法
河本宏; 金子新; 増田喬子; 南川淳隆; 堀田秋津; 島津裕; 一瀬大志

特開2020-141695, 特願2020-089304, 多能性幹細胞から免疫細胞療法用T細胞を誘導する方法
河本宏; 金子新; 増田喬子; 南川淳隆; 堀田秋津; 島津裕; 一瀬大志

WO2020-122104, JP2019048426, ゲノムDNAに欠失を誘導する方法
堀田秋津; 奥嵜雄也; 徐淮耕; ジーピーターデビド; 北悠人; 真下知士; 吉見一人

特許第6715482号, 特願2016-534507, 多能性幹細胞から免疫細胞療法用T細胞を誘導する方法
金子新; 河本宏; 増田喬子; 南川淳隆; 堀田秋津; 島津裕; 一瀬大志

WO2020-111229, JP2019046777, 溶液を用いた薬物送達系
堀田秋津; 石原直子; 西垣竜一; 瀬藤正記

WO2020-013317, JP2019027708, ウイルス様粒子及びその使用
堀田秋津; ジーピーターデビド

WO2019-160077, JP2019005524, 低抗原性細胞の製造方法
堀田秋津; 徐淮耕; 金子新; 王博

WO2019-131829, JP2018048034, 標的遺伝子改変用組成物
堀田秋津; 井福正隆; 藤本直子; 岩渕久美子; 見城江利也; 蒔田幸正; 落合留美子

WO2019-017321, JP2018026676, 遺伝子変異導入方法
堀田秋津; 石田賢太郎

WO2018-179578, JP2017041756, ゲノム編集によるエクソンスキッピング誘導方法
李紅梅; 笹川典子; 堀田秋津

WO2016-010148, JP2015070608, 多能性幹細胞から免疫細胞療法用T細胞を誘導する方法
金子新; 河本宏; 増田喬子; 南川淳隆; 堀田秋津; 島津裕; 一瀬大志

WO2020/013317, 特願PCT/JP2019/027708, ウイルス様粒子及びその使用
堀田秋津; Peter Gee

WO2022/137760, 特願PCT/JP2019/48426, ゲノムＤＮＡに欠失を誘導する方法
堀田秋津; 奥嵜雄也; 徐淮耕; 真下知士; 吉見一人; Peter Gee; 北悠人

WO2019/017321, 特願PCT/JP2018/26676, 遺伝子変異導入方法
堀田秋津; 石田賢太郎

WO2018/179578, 特願PCT/JP2017/41756, ゲノム編集によるエクソンスキッピング誘導方法
堀田秋津; 李紅梅; 笹川典子

WO2019/160077, 特願2018-026421, 低抗原性細胞の製造方法
堀田秋津; 徐淮耕; 金子新; 王博

WO2016/010148, JP2015070608, 多能性幹細胞から免疫細胞療法用T細胞を誘導する方法
金子新; 河本宏; 増田喬子; 南川淳隆; 堀田秋津; 島津裕; 一瀬大志

US20110053166A1, Stem cell expression cassettes
James Ellis; Akitsu Hotta; Dixie Mager

Awards

2008
カナダ幹細胞ネットワーク（SCN）年次大会, Best Poster Presentation Award

2009
トロント小児病院（Sickkids）, Exceptional Trainee Award

12 Apr. 2016
文部科学省, 科学技術分野の文部科学大臣表彰若手科学者賞

May 2020
日本再生医療学会, 日本再生医療学会賞　基礎部門

External funds: Kakenhi

Development of induciblydiversified antibody-expressing cell library that enables antibody phenotypic screening
Grant-in-Aid for Scientific Research (B)
Basic Section 38060:Applied molecular and cellular biology-related
Tokyo University of Pharmacy and Life Science
KAZUMA TOMIZUKA
From 01 Apr. 2019, To 31 Mar. 2022, Project Closed
ゲノム編集;ヒト抗体;相補性決定領域;Wnt/βカテニンシグナル;ペプチド医薬;指向性進化;抗体医薬;ヒト人工染色体;免疫グロブリン;CRISPR-Cas9;Ｗｎｔ/βカテニンシグナル;表現型スクリーニング;染色体工学;モノクローナル抗体;進化工学
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H02924/

Precise genomic correction by intercellular regulation of genome editing enzyme
Grant-in-Aid for Young Scientists (A)
Kyoto University
Akitsu Hotta
From 01 Apr. 2015, To 31 Mar. 2018, Project Closed
ゲノム編集;iPS細胞;CRISPR;相同組換え;一塩基修復;一塩基変異;一塩基置換;CRISPR-Cas9;遺伝子治療
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15H05581/

Application of iPS cell technology to lung regeneration and development of bioresource for elucidating the mechanisms of lung diseases
Grant-in-Aid for Scientific Research (A)
Kyoto University
Mishima Michiaki
From 01 Apr. 2015, To 31 Mar. 2018, Project Closed
分子細胞呼吸学;iPS細胞;肺臓器再生;疾患モデリング;分子細胞呼吸器学
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15H02537/

Establishment of the next generation treatment for hemophilia A by autologous cell implantation
Grant-in-Aid for Scientific Research (B)
Nara Medical University
Modori Shima
From 01 Apr. 2013, To 31 Mar. 2016, Project Closed
hemophilia A;gene therapy;piggyBack;transposon;factor Ⅷ;血友病Ａ;遺伝子治療;細胞治療;血友病A;自己細胞;細胞移植;免疫応答
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25293237/

Novel shRNA library screening to identify key factors that are important in pluripotent stem cells
Grant-in-Aid for Young Scientists (B)
Kyoto University
Akitsu HOTTA
From 01 Apr. 2012, To 31 Mar. 2015, Project Closed
shRNAライブラリー;トランスポゾンベクター;発現抑制制御;発現抑制
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24770206/

Establishment of immune torelance induction therapy for FVIII inhibitors by subcutaneous implantation of blood outgrowth endothelial cells
Grant-in-Aid for Scientific Research (C)
Nara Medical University
HIDETO MATSUI
From 01 Apr. 2012, To 31 Mar. 2015, Project Closed
血友病;インヒビター;細胞移植;免疫寛容;制御性T細胞;免疫寛容導入;遺伝子・細胞治療;血友病A;遺伝子治療;細胞治療;細胞療法
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24591559/

Development of non-viral vector for gene therapy strategy using iPS cells
Grant-in-Aid for Research Activity Start-up
Kyoto University
Akitsu HOTTA
From 01 Apr. 2010, To 31 Mar. 2012, Project Closed
遺伝子治療;iPS細胞;遺伝子導入ベクター;血友病A;遺伝子導入;サイレンシング
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22890088/

近傍集積因子解析よるCRISPR-Cas3ゲノム欠失誘導機構の解明
Grant-in-Aid for Scientific Research (B)
Basic Section 43050:Genome biology-related
Kyoto University
堀田秋津
From 01 Apr. 2022, To 31 Mar. 2025, Granted
CRISPR-Cas3;ゲノム編集;DNA損傷修復
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H02601/

ゲノム編集誘導性分子多様化による、機能抗体・ペプチド表現型スクリーニングシステム
Grant-in-Aid for Scientific Research (B)
Basic Section 47010:Pharmaceutical chemistry and drug development sciences-related
Tokyo University of Pharmacy and Life Science
冨塚一磨
From 01 Apr. 2023, To 31 Mar. 2027, Granted
ゲノム編集;表現型スクリーニング;機能抗体;分子進化
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23H02615/

近傍集積因子解析よるCRISPR-Cas3ゲノム欠失誘導機構の解明
Grant-in-Aid for Scientific Research (B)
Basic Section 43050:Genome biology-related
Kyoto University
堀田秋津
From 01 Apr. 2022, To 31 Mar. 2025, Granted
ゲノム編集;CRISPR-Cas3;DNA損傷修復
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K23864/

ゲノム編集誘導性分子多様化による、機能抗体・ペプチド表現型スクリーニングシステム
Grant-in-Aid for Scientific Research (B)
Basic Section 47010:Pharmaceutical chemistry and drug development sciences-related
Tokyo University of Pharmacy and Life Science
冨塚一磨
From 01 Apr. 2023, To 31 Mar. 2027, Granted
ゲノム編集;表現型スクリーニング;機能抗体;分子進化
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K27306/

宿主小胞形成因子を活用したCRISPR送達法の開発とその応用
Grant-in-Aid for JSPS Fellows
Basic Section 90110:Biomedical engineering-related
Kyoto University
堀田秋津
From 01 Oct. 2024, To 31 Mar. 2027, Adopted
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24KF0157/

External funds: others

血友病の新規iPS細胞遺伝子治療戦略
上原記念生命科学財団
From 19 Jan. 2011, To 31 Mar. 2012
堀田秋津

iPS細胞での長期安定発現を可能とするヒト由来インシュレーターの活用および作用機構解明
持田記念研究助成金
From 28 Sep. 2012, To 31 Mar. 2013
堀田秋津

iPS細胞を用いた次世代型血友病治療の創出
バクスター　凝固関連研究基金（ＢＣＲＦ）
From Jan. 2013, To 28 Feb. 2014
堀田秋津

人為的核内環境制御による高品質iPS細胞の誘導
独立行政法人科学技術振興機構さきがけ
From 01 Oct. 2010, To 31 Mar. 2014
堀田秋津

心機能再生を目指した特定因子による細胞変換技術開発
独立行政法人科学技術振興機構　再生医療実現拠点ネットワークプログラム　技術開発個別課題
From 08 Jul. 2013, To 31 Mar. 2014
竹内純

再生医療用iPS細胞ストック開発拠点
独立行政法人科学技術振興機構　再生医療実現拠点ネットワークプログラム　中核拠点
From 08 Jul. 2013, To 31 Mar. 2023
山中伸弥

筋疾患に対する治療薬の創出を目指した研究
国立研究開発法人日本医療研究開発機構　再生医療実現拠点ネットワークプログラム（疾患特異的iPS細胞の利活用促進・難病研究加速プログラム）
From 29 Aug. 2017, To 31 Mar. 2022
櫻井英俊

独自送達技術開発による先天性筋疾患に対するゲノム編集治療法の開発
国立研究開発法人日本医療研究開発機構　難治性疾患実用化研究事業
From 29 Nov. 2017, To 31 Mar. 2020
堀田秋津

Last Updated :2025/04/23

Education

Teaching subject(s)

From 01 Apr. 2024, To 31 Mar. 2025
ILAS Seminar
Z001, Spring, Institute for Liberal Arts and Sciences, 2

From 01 Apr. 2023, To 31 Mar. 2024
ILAS Seminar
Z001, Spring, Institute for Liberal Arts and Sciences, 2

From 01 Apr. 2022, To 31 Mar. 2023
ILAS Seminar
Z001, Spring, Institute for Liberal Arts and Sciences, 2

From Apr. 2014, To Mar. 2015
An introduction to iPS cells
Spring, 全学共通科目

From Apr. 2015, To Mar. 2016
An introduction to iPS cells
Spring, 全学共通科目

From Apr. 2016, To Mar. 2017
ILAS Seminar
Spring, 全学共通科目

From Apr. 2017, To Mar. 2018
ILAS Seminar
Spring, 全学共通科目

From Apr. 2018, To Mar. 2019
ILAS Seminar
Spring, 全学共通科目

From Apr. 2019, To Mar. 2020
ILAS Seminar
Spring, 全学共通科目

From Apr. 2020, To Mar. 2021
ILAS Seminar :Introduction to iPS cells
Spring, 全学共通科目

From Apr. 2021, To Mar. 2022
ILAS Seminar :Introduction to iPS cell research
Spring, 全学共通科目

Last Updated :2025/04/23

Administration

School management (title, position)

組換えDNA実験安全副主任者

Faculty management (title, position)

組換えDNA実験安全委員会

From 01 Apr. 2015, To 31 Mar. 2026
情報管理委員会

From 29 Sep. 2017
倫理審査委員会

From 01 Apr. 2016, To 31 Mar. 2026
研究インターンシップ委員会

From 01 Apr. 2020, To 31 Mar. 2026
財務委員会1号委員

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Hotta, Akitsu

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External funds: Kakenhi

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