Basic Information

Faculty

• 農学部

Academic Degree

• 理学修士(東京大学)
• 医学博士(大阪大学)

Academic Resume (Graduate Schools)

• 東京大学, 大学院理学系研究科修士課程生物化学専攻, 修了

ID,URL

• J-Global ID

  201401071053492412

researchmap URL

• https://researchmap.jp/7000008475

Research

Research Topics, Overview of the research

• Research Topics

  Generation and application of enzyme functions

• Overview of the research

  We are aiming at elucidating the relationship between structure and function of enzymes at a molecular level and developing new methods for application of enzymes in food and medical technologies by generating new enzyme functions and controlling enzyme reactions.

Research Areas

• Life sciences, Applied biochemistry

Papers

• Thermotoga maritimaピルビン酸キナーゼのリコンビナーゼポリメラーゼ増幅法への応用

  Juma Kevin Maafu; 村上 雄人; 森本 健太; 滝田 禎亮; 兒島 憲二; 鈴木 孝一朗; 柳原 格; 生田 宗一郎; 藤原 伸介; 保川 清

  Journal of Biological Macromolecules, Jul. 2024

  
• Effects of amino acid substitutions at position 315 of GH10 xylanase XynR on its alkaliphily

  Tomoka Nakamura; Kohei Kuwata; Teisuke Takita; Kimihiko Mizutani; Bunzo Mikami; Satoshi Nakamura; Kiyoshi Yasukawa

  Journal of Biological Macromolecules, 01 Mar. 2024, Peer-reviewed

  
• Structure-Function Analysis of Streptomyces griseolus CYP105A1 in the Metabolism of Nonsteroidal Anti-inflammatory Drugs.

  Teisuke Takita; Moeka Wada; Masaya Yamagata; Seiei Kamata; Kimihiko Mizutani; Yuya Yogo; Masahiro Hamada; Kaori Yasuda; Bunzo Mikami; Toshiyuki Sakaki; Kiyoshi Yasukawa

  Biochemistry, 03 Jan. 2025

  
• Expression of Recombinant Human α-Glucosidase in HEK293 Cells

  So Nishimoto; Anaïs Debarbat; Yuki Ikeda; Emi Arikawa; Yuki Odagaki; Haruna Yano; Ying Qiao; Masaaki Ito; Toshiyuki Kimura; Teisuke Takita; Kiyoshi Yasukawa

  Journal of Agricultural and Food Chemistry, 23 Dec. 2024

  
• Unveiling the reaction mechanism of arginine decarboxylase in Aspergillus oryzae: Insights from crystal structure analysis.

  Yuki Odagaki; Yui Murakami; Teisuke Takita; Kimihiko Mizutani; Bunzo Mikami; Shinsuke Fujiwara; Kiyoshi Yasukawa

  Biochemical and biophysical research communications, 12 Nov. 2024

  
• Use of human Caco-2 cells and HPAE-PAD for α-glucosidase assay

  Yuki Ikeda; So Nishimoto; Ying Qiao; Haruna Yano; Hideaki Minami; Masaaki Ito; Toshiyuki Kimura; Teisuke Takita; Kiyoshi Yasukawa

  Journal of Pharmacological and Toxicological Methods, May 2024

  
• Alteration of enzymes and their application to nucleic acid amplification (Review)

  Kiyoshi Yasukawa; Itaru Yanagihara; Shinsuke Fujiwara

  International Journal of Molecular Medicine, 01 Nov. 2020

  
• 放線菌由来P450(CYP105A1)の機能改変と医薬品代謝研究への応用 R84A変異体による非ステロイド性抗炎症薬(NSAIDs)の代謝

  鎌田 成栄; 和田 萌加; 山形 昌也; 水谷 公彦; 三上 文三; 大橋 錬; 佐久間 宙; 根本 翔; Nilouyal Somaye; 余語 祐哉; 安田 佳織; 濱田 昌弘; 榊 利之; 保川 清; 滝田 禎亮

  Journal of Biological Macromolecules, Aug. 2023

  
• Geobacillus stearothermophilus由来トランスグルタミナーゼ(GsTgl)のC末端伸長配列の役割の解明とX線結晶構造解析

  濱中 雄大; 京 逸如; 雷 雨坤; 山田 章文; 三上 文三; 水谷 公彦; 保川 清; 滝田 禎亮

  Journal of Biological Macromolecules, Aug. 2022

  
• Achieving unprecedented stability in lyophilized recombinase polymerase amplification with thermostable pyruvate kinase from Thermotoga maritima.

  Kevin Maafu Juma; Yuto Murakami; Kenta Morimoto; Teisuke Takita; Kenji Kojima; Koichiro Suzuki; Itaru Yanagihara; Soichiro Ikuta; Shinsuke Fujiwara; Kiyoshi Yasukawa

  Journal of bioscience and bioengineering, 07 May 2024

  
• Activity-stability trade-off observed in variants at position 315 of the GH10 xylanase XynR.

  Tomoka Nakamura; Teisuke Takita; Kohei Kuwata; Kimihiko Mizutani; Bunzo Mikami; Satoshi Nakamura; Kiyoshi Yasukawa

  Scientific reports, 02 Apr. 2024

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• Increase in the solubility of uvsY using a site saturation mutagenesis library for application in a lyophilized reagent for recombinase polymerase amplification.

  Kenta Morimoto; Kevin Maafu Juma; Masaya Yamagata; Teisuke Takita; Kenji Kojima; Koichiro Suzuki; Itaru Yanagihara; Shinsuke Fujiwara; Kiyoshi Yasukawa

  Molecular biology reports, 27 Feb. 2024

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• Insights into the catalytic mechanism of Grimontia hollisae collagenase through structural and mutational analyses.

  Saori Ueshima; Mizuki Yasumoto; Yuto Kitagawa; Kaho Akazawa; Teisuke Takita; Keisuke Tanaka; Shunji Hattori; Kimihiko Mizutani; Bunzo Mikami; Kiyoshi Yasukawa

  FEBS letters, Oct. 2023

  
• Recombinase polymerase amplification using novel thermostable strand-displacing DNA polymerases from Aeribacillus pallidus and Geobacillus zalihae.

  Kevin Maafu Juma; Eisuke Inoue; Kengo Asada; Wakao Fukuda; Kenta Morimoto; Masaya Yamagata; Teisuke Takita; Kenji Kojima; Koichiro Suzuki; Yukiko Nakura; Itaru Yanagihara; Shinsuke Fujiwara; Kiyoshi Yasukawa

  Journal of bioscience and bioengineering, 17 Feb. 2023

  
• Inhibition of α-amylase and α-glucosidase by Morus australis fruit extract and its components iminosugar, anthocyanin, and glucose

  Ying Qiao; Yuki Ikeda; Masaaki Ito; Toshiyuki Kimura; Takeaki Ikeuchi; Teisuke Takita; Kiyoshi Yasukawa

  Journal of Food Science, Apr. 2022, Peer-reviewed

  
• Characterization of proteases activities in Ficus carica cultivars.

  Kosaku Nishimura; Keisuke Higashiya; Naoki Ueshima; Tatsuya Abe; Kiyoshi Yasukawa

  Journal of food science, Mar. 2020

  
• Crystal structure of Grimontia hollisae collagenase provides insights into its novel substrate specificity toward collagen.

  Takeaki Ikeuchi; Mizuki Yasumoto; Teisuke Takita; Keisuke Tanaka; Masashi Kusubata; Osamu Hayashida; Shunji Hattori; Kimihiko Mizutani; Bunzo Mikami; Kiyoshi Yasukawa

  The Journal of biological chemistry, 06 Jun. 2022

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• Modified uvsY by N-terminal hexahistidine tag addition enhances efficiency of recombinase polymerase amplification to detect SARS-CoV-2 DNA.

  Kevin Maafu Juma; Teisuke Takita; Masaya Yamagata; Mika Ishitani; Kaichi Hayashi; Kenji Kojima; Koichiro Suzuki; Yuri Ando; Wakao Fukuda; Shinsuke Fujiwara; Yukiko Nakura; Itaru Yanagihara; Kiyoshi Yasukawa

  Molecular biology reports, Apr. 2022

  
• Comparison of the stability of CYP105A1 and its variants engineered for production of active forms of vitamin D.

  Teisuke Takita; Hiro Sakuma; Ren Ohashi; Somaye Nilouyal; Sho Nemoto; Moeka Wada; Yuya Yogo; Kaori Yasuda; Shinichi Ikushiro; Toshiyuki Sakaki; Kiyoshi Yasukawa

  Bioscience, biotechnology, and biochemistry, 21 Mar. 2022, Peer-reviewed

  
• Metabolism of non-steroidal anti-inflammatory drugs (NSAIDs) by Streptomyces griseolus CYP105A1 and its variants.

  Yuya Yogo; Kaori Yasuda; Teisuke Takita; Kiyoshi Yasukawa; Yuichiro Iwai; Miyu Nishikawa; Hiroshi Sugimoto; Shinichi Ikushiro; Toshiyuki Sakaki

  Drug metabolism and pharmacokinetics, 07 Mar. 2022

  
• Optimization of reaction condition of recombinase polymerase amplification to detect SARS-CoV-2 DNA and RNA using a statistical method

  Kevin Maafu Juma; Teisuke Takita; Kenji Ito; Masaya Yamagata; Shihomi Akagi; Emi Arikawa; Kenji Kojima; Manish Biyani; Shinsuke Fujiwara; Yukiko Nakura; Itaru Yanagihara; Kiyoshi Yasukawa

  Biochemical and Biophysical Research Communications, Aug. 2021, Peer-reviewed, Corresponding author

  
• Development of an efficient one-step real-time reverse transcription polymerase chain reaction method for severe acute respiratory syndrome-coronavirus-2 detection

  Yukiko Nakura; Heng Ning Wu; Yuya Okamoto; Muneyuki Takeuchi; Koichiro Suzuki; Yoshitaka Tamura; Yuichiro Oba; Fumiko Nishiumi; Nobuaki Hatori; Shinsuke Fujiwara; Kiyoshi Yasukawa; Shinobu Ida; Itaru Yanagihara

  PLOS ONE, 04 Jun. 2021, Peer-reviewed

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• The mutation of Thr315 to Asn of GH10 xylanase XynR increases the alkaliphily but decreases the alkaline resistance

  Kohei Kuwata; Manami Suzuki; Teisuke Takita; Rie Yatsunami; Satoshi Nakamura; Kiyoshi Yasukawa

  Bioscience, Biotechnology, and Biochemistry, 02 Jun. 2021, Peer-reviewed, Corresponding author

  
• Inhibitory effect of Morus australis leaf extract and its component iminosugars on intestinal carbohydrate-digesting enzymes

  Ying Qiao; Masaaki Ito; Toshiyuki Kimura; Takeaki Ikeuchi; Teisuke Takita; Kiyoshi Yasukawa

  Journal of Bioscience and Bioengineering, Jun. 2021, Peer-reviewed, Corresponding author

  
• Insight into the collagen-degrading activity of a serine protease in the latex of Ficus carica cultivar Masui Dauphine

  Kosaku Nishimura; Keisuke Higashiya; Naoki Ueshima; Kenji Kojima; Teisuke Takita; Tatsuya Abe; Toru Takahashi; Kiyoshi Yasukawa

  Bioscience, Biotechnology, and Biochemistry, 24 Apr. 2021, Peer-reviewed, Corresponding author

  
• Insight into the mechanism of thermostabilization of GH10 xylanase from Bacillus sp. strain TAR-1 by the mutation of S92 to E

  Manami Suzuki; Teisuke Takita; Kohei Kuwata; Kota Nakatani; Tongyang Li; Yuta Katano; Kenji Kojima; Kimihiko Mizutani; Bunzo Mikami; Rie Yatsunami; Satoshi Nakamura; Kiyoshi Yasukawa

  Bioscience, Biotechnology, and Biochemistry, 18 Feb. 2021, Peer-reviewed, Corresponding author

  
• Improvement of Moloney murine leukemia virus reverse transcriptase thermostability by introducing a disulfide bridge in the ribonuclease H region

  Yutaro Narukawa; Mako Kandabashi; Tongyang Li; Misato Baba; Haruka Hara; Kenji Kojima; Kei Iida; Takayoshi Hiyama; Sho Yokoe; Tomomi Yamazaki; Teisuke Takita; Kiyoshi Yasukawa

  Protein Engineering, Design and Selection, 15 Feb. 2021, Peer-reviewed, Corresponding author

  
• Solvent engineering studies on recombinase polymerase amplification

  Kenji Kojima; Kevin Maafu Juma; Shihomi Akagi; Kaichi Hayashi; Teisuke Takita; Ciara K. O'Sullivan; Shinsuke Fujiwara; Yukiko Nakura; Itaru Yanagihara; Kiyoshi Yasukawa

  Journal of Bioscience and Bioengineering, Feb. 2021, Peer-reviewed, Corresponding author

  
• Comparison of sensitivity and rapidness of PCR, recombinase polymerase amplification, and RNA-specific amplification for detection of Rice yellow mottle virus

  Kevin Maafu Juma; Kenji Kojima; Teisuke Takita; Keiko T. Natsuaki; Kiyoshi Yasukawa

  Journal of Biological Macromolecules, 2021

  
• Kinetic analysis of inhibition of α-glucosidase by leaf powder from Morus australis and its component iminosugars

  Ying Qiao; Juri Nakayama; Takeaki Ikeuchi; Masaaki Ito; Toshiyuki Kimura; Kenji Kojima; Teisuke Takita; Kiyoshi Yasukawa

  Bioscience, Biotechnology, and Biochemistry, 13 Jul. 2020, Peer-reviewed

  
• The roles of histidine and tyrosine residues in the active site of collagenase in Grimontia hollisae

  Kaichi Hayashi; Takeaki Ikeuchi; Ryo Morishita; Jun Qian; Kenji Kojima; Teisuke Takita; Keisuke Tanaka; Shunji Hattori; Kiyoshi Yasukawa

  The Journal of Biochemistry, 09 May 2020, Peer-reviewed

  
• Val143 of human ribonuclease H2 is not critical for, but plays a role in determining catalytic activity and substrate specificity

  Misato Baba; Kenji Kojima; Takuto Nishimura; Takuya Sugiura; Teisuke Takita; Ryo Uehara; Robert J. Crouch; Kiyoshi Yasukawa

  PLOS ONE, 18 Feb. 2020, Peer-reviewed

  
• Inhibition of HIV-1 Reverse Transcriptase Activity by the Extracts of Indian Plants

  Keiko Ishizuka; Yuto Tsutsumi; Misato Baba; Radhika Biyani; Chen Wei Meng; Manish Biyani; Masahiro Takagi; Jyotsna Jaiswal; Bechan Sharma; Kenji Kojima; Teisuke Takita; Kiyoshi Yasukawa

  Journal of Biological Macromolecules, 2020

  
• Characterization of six recombinant human RNase H2 bearing Aicardi-Goutiéres syndrome causing mutations

  Takuto Nishimura; Misato Baba; Saori Ogawa; Kenji Kojima; Teisuke Takita; Robert J Crouch; Kiyoshi Yasukawa

  The Journal of Biochemistry, 01 Dec. 2019, Peer-reviewed

  
• Increase in the thermostability of GH11 xylanase XynJ from Bacillus sp. strain 41M-1 using site saturation mutagenesis.

  Teisuke Takita; Kota Nakatani; Yuta Katano; Manami Suzuki; Kenji Kojima; Naoki Saka; Bunzo Mikami; Rie Yatsunami; Satoshi Nakamura; Kiyoshi Yasukawa

  Enzyme and microbial technology, Nov. 2019, Peer-reviewed

  
• Construction and characterization of ribonuclease H2 knockout NIH3T3 cells.

  Motoki Tsukiashi; Misato Baba; Kenji Kojima; Kohei Himeda; Teisuke Takita; Kiyoshi Yasukawa

  Journal of biochemistry, 01 Mar. 2019, Peer-reviewed

  
• Increase in the thermostability of Bacillus sp. strain TAR-1 xylanase using a site saturation mutagenesis library.

  Kota Nakatani; Yuta Katano; Kenji Kojima; Teisuke Takita; Rie Yatsunami; Satoshi Nakamura; Kiyoshi Yasukawa

  Bioscience, biotechnology, and biochemistry, Oct. 2018, Peer-reviewed

  
• Accurate fidelity analysis of the reverse transcriptase by a modified next-generation sequencing.

  Hiroyuki Okano; Misato Baba; Ryota Hidese; Kei Iida; Tongyang Li; Kenji Kojima; Teisuke Takita; Itaru Yanagihara; Shinsuke Fujiwara; Kiyoshi Yasukawa

  Enzyme and microbial technology, Aug. 2018, Peer-reviewed

  
• RNA/DNA structures recognized by RNase H2.

  Kenji Kojima; Misato Baba; Motoki Tsukiashi; Takuto Nishimura; Kiyoshi Yasukawa

  Briefings in functional genomics, 20 Jun. 2018, Peer-reviewed

  
• Comparative studies on the activities of collagenases from Grimontia hollisae and Clostridium hystoliticum in the hydrolysis of synthetic substrates.

  Teisuke Takita; Jun Qian; Hongmin Geng; Zejian He; Sho Nemoto; Mariko Mori; Keisuke Tanaka; Shunji Hattori; Kenji Kojima; Kiyoshi Yasukawa

  Journal of biochemistry, 01 May 2018, Peer-reviewed

  
• High sensitive RNA detection by one-step RT-PCR using the genetically engineered variant of DNA polymerase with reverse transcriptase activity from hyperthermophilies.

  Hiroyuki Okano; Misato Baba; Katsuhiro Kawato; Ryota Hidese; Itaru Yanagihara; Kenji Kojima; Teisuke Takita; Shinsuke Fujiwara; Kiyoshi Yasukawa

  Journal of bioscience and bioengineering, Mar. 2018, Peer-reviewed

  
• Thermostable DNA helicase improves the sensitivity of digital PCR

  Ryota Hidese; Katsuhiro Kawato; Yukiko Nakura; Ayako Fujiwara; Kiyoshi Yasukawa; Itaru Yanagihara; Shinsuke Fujiwara

  Biochemical and Biophysical Research Communications, 15 Jan. 2018, Peer-reviewed

  
• Protein engineering of CYP105s for their industrial uses

  Kaori Yasuda; Hiroshi Sugimoto; Keiko Hayashi; Teisuke Takita; Kiyoshi Yasukawa; Miho Ohta; Masaki Kamakura; Shinichi Ikushiro; Yoshitsugu Shiro; Toshiyuki Sakaki

  BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Jan. 2018, Peer-reviewed

  
• Knockout of ribonuclease H2 increases ribonucleotides in the genome DNA of NIH3T3 cells

  Tsukiashi, M; Baba, M; Kojima, K; Himeda, K; Teisuke T; Yasukawa, K

  J. Biochem., 2018, Peer-reviewed

  
• DNA-based mutation assay GPMA (genome profiling-based mutation assay): reproducibility, parts-per-billion scale sensitivity, and introduction of a mammalian-cell-based approach

  Parmila Kumari; Sunita Ghimire Gautam; Misato Baba; Motoki Tsukiashi; Koji Matsuoka; Kiyoshi Yasukawa; Koichi Nishigaki

  JOURNAL OF BIOCHEMISTRY, Dec. 2017, Peer-reviewed

  
• Generation of thermostable Moloney murine leukemia virus reverse transcriptase variants using site saturation mutagenesis library and cell-free protein expression system.

  Yuta Katano; Tongyang Li; Misato Baba; Miyo Nakamura; Masaaki Ito; Kenji Kojima; Teisuke Takita; Kiyoshi Yasukawa

  Bioscience, biotechnology, and biochemistry, Dec. 2017, Peer-reviewed

  
• Next-generation sequencing-based analysis of reverse transcriptase fidelity

  Kiyoshi Yasukawa; Kei Iida; Hiroyuki Okano; Ryota Hidese; Misato Baba; Itaru Yanagihara; Kenji Kojima; Teisuke Takita; Shinsuke Fujiwara

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Oct. 2017, Peer-reviewed

  
• Effects of neutral salts and pH on the activity and stability of human RNase H2

  Misato Baba; Kenji Kojima; Rihoko Nakase; Shota Imai; Tomomi Yamasaki; Teisuke Takita; Robert J. Crouch; Kiyoshi Yasukawa

  JOURNAL OF BIOCHEMISTRY, Sep. 2017, Peer-reviewed

  
• Further increase in thermostability of Moloney murine leukemia virus reverse transcriptase by mutational combination

  Misato Baba; Ryota Kakue; Christoph Leucht; Peter Rasor; Heiko Walch; Daniel Ladiges; Christian Bell; Kenji Kojima; Teisuke Takita; Kiyoshi Yasukawa

  PROTEIN ENGINEERING DESIGN & SELECTION, Aug. 2017, Peer-reviewed

  
• High sensitive one-step RT-PCR using MMLV reverse transcriptase, DNA polymerase with reverse transcriptase activity, and DNA/RNA helicase

  Hiroyuki Okano; Misato Baba; Tomomi Yamasaki; Ryota Hidese; Shinsuke Fujiwara; Itaru Yanagihara; Takeshi Ujiiye; Tsukasa Hayashi; Kenji Kojima; Teisuke Takita; Kiyoshi Yasukawa

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, May 2017, Peer-reviewed

  
• Production of an active form of vitamin D2 by genetically engineered CYP105A1

  Kaori Yasuda; Yuya Yogo; Hiroshi Sugimoto; Hiroki Mano; Teisuke Takita; Miho Ohta; Masaki Kamakura; Shinichi Ikushiro; Kiyoshi Yasukawa; Yoshitsugu Shiro; Toshiyuki Sakaki

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Apr. 2017, Peer-reviewed

  
• The cleavage site preference of the porcine pepsin on the N-terminal 1 chain of bovine type I collagen: a focal analysis with mass spectrometry

  Jun Qian; Shinji Ito; Junko Satoh; Hongmin Geng; Keisuke Tanaka; Shunji Hattori; Kenji Kojima; Teisuke Takita; Kiyoshi Yasukawa

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Mar. 2017, Peer-reviewed

  
• Development of immersion vaccine for bacterial cold-water disease in ayu Plecoglossus altivelis

  Hitoshi Nakayama; Mariko Mori; Teisuke Takita; Kiyoshi Yasukawa; Keisuke Tanaka; Shunji Hattori; Hideaki Aikawa; Osamu Hasegawa; Takashi Okamura; Kentarou Takegami; Shogo Motokawa; Masakazu Kuwahara; Kenichi Amano

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Mar. 2017, Peer-reviewed

  
• DNA-based mutation assay (GPMA) can be of ppb-order sensitivity for both bacterial and mammalian testers

  Kumari, P; Gautam, S.G; Baba, M; Tsukiashi, M; Matsuoka, K; Yasukawa, K; Nishigaki, K

  J. Biochem., 2017, Peer-reviewed

  
• Enhanced detection of RNA by MMLV reverse transcriptase coupled with thermostable DNA polymerase and DNA/RNA helicase

  Hiroyuki Okano; Yuta Katano; Misato Baba; Ayako Fujiwara; Ryota Hidese; Shinsuke Fujiwara; Itaru Yanagihara; Tsukasa Hayashi; Kenji Kojima; Teisuke Takita; Kiyoshi Yasukawa

  ENZYME AND MICROBIAL TECHNOLOGY, Jan. 2017, Peer-reviewed

  
• Expression of moloney murine leukemia virus reverse transcriptase in a cell-free protein expression system

  Yuta Katano; Tetsuro Hisayoshi; Ikumi Kuze; Hiroyuki Okano; Masaaki Ito; Koichi Nishigaki; Teisuke Takita; Kiyoshi Yasukawa

  BIOTECHNOLOGY LETTERS, Jul. 2016, Peer-reviewed

  
• Application of a Euryarchaeota-Specific Helicase from Thermococcus kodakarensis for Noise Reduction in PCR

  Ayako Fujiwara; Katsuhiro Kawato; Saori Kato; Kiyoshi Yasukawa; Ryota Hidese; Shinsuke Fujiwara

  APPLIED AND ENVIRONMENTAL MICROBIOLOGY, May 2016, Peer-reviewed

  
• Sequential hydroxylation of vitamin D-2 by a genetically engineered CYP105A1

  Keiko Hayashi; Kaori Yasuda; Yuya Yogo; Teisuke Takita; Kiyoshi Yasukawa; Miho Ohta; Masaki Kamakura; Shinichi Ikushiro; Toshiyuki Sakaki

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, May 2016, Peer-reviewed

  
• Application of a Euryarchaeota-specific helicase from Thermococcus kodakarensis for noise reduction in PCR

  Ayako Fujiwara; Katsuhiro Kawato; Saori Kato; Kiyoshi Yasukawa; Ryota Hidese; Shinsuke Fujiwara

  Applied and Environmental Microbiology, 01 May 2016, Peer-reviewed

  
• Kinetic Analysis of the Digestion of Bovine Type I Collagen Telopeptides with Porcine Pepsin

  Jun Qian; Yukari Okada; Takayuki Ogura; Keisuke Tanaka; Shunji Hattori; Shinji Ito; Junko Satoh; Teisuke Takita; Kiyoshi Yasukawa

  JOURNAL OF FOOD SCIENCE, Jan. 2016, Peer-reviewed

  
• Preparation and characterization of the RNase H domain of Moloney murine leukemia virus reverse transcriptase

  Kosaku Nishimura; Kanta Yokokawa; Tetsuro Hisayoshi; Kosuke Fukatsu; Ikumi Kuze; Atsushi Konishi; Bunzo Mikami; Kenji Kojima; Kiyoshi Yasukawa

  PROTEIN EXPRESSION AND PURIFICATION, Sep. 2015, Peer-reviewed

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• Inhibition of the DNA polymerase and RNase H activities of HIV-1 reverse transcriptase and HIV-1 replication by Brasenia schreberi (Junsai) and Petasites japonicus (Fuki) components

  Tetsuro Hisayoshi; Mayu Shinomura; Kanta Yokokawa; Ikumi Kuze; Atsushi Konishi; Kumi Kawaji; Eiichi N. Kodama; Keishi Hata; Saori Takahashi; Satoru Nirasawa; Shohei Sakuda; Kiyoshi Yasukawa

  JOURNAL OF NATURAL MEDICINES, Jul. 2015, Peer-reviewed

  
• Enzymatic Activities of RNase H Domains of HIV-1 Reverse Transcriptase with Substrate Binding Domains of Bacterial RNases H1 and H2

  Etin-Diah Permanasari; Kiyoshi Yasukawa; Shigenori Kanaya

  MOLECULAR BIOTECHNOLOGY, Jun. 2015, Peer-reviewed

  
• Amino acid substitutions away from the RNase H catalytic site increase the thermal stability of Moloney murine leukemia virus reverse transcriptase through RNase H inactivation

  Atsushi Konishi; Tetsuro Hisayoshi; Kanta Yokokawa; Veronica Barrioluengo; Luis Menendez-Arias; Kiyoshi Yasukawa

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Nov. 2014, Peer-reviewed

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• Effects of salts on the interaction of 8-anilinonaphthalene 1-sulphonate and thermolysin

  Vimbai Samukange; Masayuki Kamo; Kiyoshi Yasukawa; Kuniyo Inouye

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Sep. 2014, Peer-reviewed

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• Inhibition of HIV-1 reverse transcriptase activity by Brasenia schreberi (Junsai) components

  Hisayoshi, T; Shinomura, M; Konishi, A; Tanaka, J; Shimoda, H; Hata, K; Takahashi, S; Yasukawa, K

  J. Biol. Macromol., 2014, Peer-reviewed

  
• Stabilization of Moloney murine leukemia virus reverse transcriptase by site-directed mutagenesis of surface residue Val433

  Atsushi Konishi; Xiaochen Ma; Kiyoshi Yasukawa

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Jan. 2014, Peer-reviewed

  
• Effects of heparin and cholesterol sulfate on the activity and stability of human matrix metalloproteinase 7

  Vimbai Samukange; Kiyoshi Yasukawa; Kuniyo Inouye

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Jan. 2014, Peer-reviewed

  
• Stabilization of human immunodeficiency virus type 1 reverse transcriptase by site-directed mutagenesis

  Kosaku Nishimura; Mayu Shinomura; Atsushi Konishi; Kiyoshi Yasukawa

  Biotechnology Letters, Dec. 2013, Peer-reviewed

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• Effects of Conversion of the Zinc-Binding Motif Sequence of Thermolysin, HEXXH, to That of Dipeptidyl Peptidase III, HEXXXH, on the Activity and Stability of Thermolysin

  Evans Menach; Yasuhiko Hashida; Kiyoshi Yasukawa; Kuniyo Inouye

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Sep. 2013, Peer-reviewed

  
• Effects of Mutations of Thermolysin, Asn116 to Asp and Asp150 to Glu, on Salt-Induced Activation and Stabilization

  Evans Menach; Kiyoshi Yasukawa; Kuniyo Inouye

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Apr. 2013, Peer-reviewed

  
• Enzymatic Characterization of Human Immunodeficiency Virus Type 1 Reverse Transcriptase for Use in cDNA Synthesis

  Atsushi Konishi; Mayu Shinomura; Kiyoshi Yasukawa

  APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, Jan. 2013, Peer-reviewed

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• Effects of site-directed mutagenesis in the N-terminal domain of thermolysin on its stabilization

  Yuichi Kawasaki; Kiyoshi Yasukawa; Kuniyo Inouye

  JOURNAL OF BIOCHEMISTRY, Jan. 2013, Peer-reviewed

  
• Kinetic analysis of reverse transcriptase activity of bacterial family A DNA polymerases

  Kiyoshi Yasukawa; Atsushi Konishi; Mayu Shinomura; Eriko Nagaoka; Shinsuke Fujiwara

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Oct. 2012, Peer-reviewed

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• Effects of site-directed mutagenesis of Asn116 in the beta-hairpin of the N-terminal domain of thermolysin on its activity and stability

  Evans Menach; Kiyoshi Yasukawa; Kuniyo Inouye

  JOURNAL OF BIOCHEMISTRY, Sep. 2012, Peer-reviewed

  
• Improving the thermal stability of avian myeloblastosis virus reverse transcriptase alpha-subunit by site-directed mutagenesis

  Atsushi Konishi; Kiyoshi Yasukawa; Kuniyo Inouye

  BIOTECHNOLOGY LETTERS, Jul. 2012, Peer-reviewed

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• Interaction of 8-anilinonaphthalene 1-sulphonate (ANS) and human matrix metalloproteinase 7 (MMP-7) as examined by MMP-7 activity and ANS fluorescence

  Vimbai Samukange; Kiyoshi Yasukawa; Kuniyo Inouye

  JOURNAL OF BIOCHEMISTRY, May 2012, Peer-reviewed

  
• A possibility of a protein-bound water molecule as the ionizable group responsible for pK(e) at the alkaline side in human matrix metalloproteinase 7 activity

  Aiko Morishima; Kiyoshi Yasukawa; Kuniyo Inouye

  JOURNAL OF BIOCHEMISTRY, May 2012, Peer-reviewed

  
• Stabilization of Bovine Intestine Alkaline Phosphatase by Sugars

  Satoshi Sekiguchi; Yasuhiko Hashida; Kiyoshi Yasukawa; Kuniyo Inouye

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Jan. 2012, Peer-reviewed

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• Thermodynamic analysis of ionizable groups involved in the catalytic mechanism of human matrix metalloproteinase 7 (MMP-7)

  Hitoshi Takeharu; Kiyoshi Yasukawa; Kuniyo Inouye

  BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Dec. 2011, Peer-reviewed

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• Effects of Polyethylene Glycol on Bovine Intestine Alkaline Phosphatase Activity and Stability

  Satoshi Sekiguchi; Kiyoshi Yasukawa; Kuniyo Inouye

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Nov. 2011, Peer-reviewed

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• Comparison of the Thermal Stabilities of the alpha beta Heterodimer and the alpha Subunit of Avian Myeloblastosis Virus Reverse Transcriptase

  Atsushi Konishi; Daisuke Nemoto; Kiyoshi Yasukawa; Kuniyo Inouye

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Aug. 2011, Peer-reviewed

  
• Analysis of the Mechanism of Inhibition of Human Matrix Metalloproteinase 7 (MMP-7) Activity by Green Tea Catechins

  Tomoko Miyake; Kiyoshi Yasukawa; Kuniyo Inouye

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Aug. 2011, Peer-reviewed

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• Effects of amines and aminoalcohols on bovine intestine alkaline phosphatase activity

  Satoshi Sekiguchi; Yasuhiko Hashida; Kiyoshi Yasukawa; Kuniyo Inouye

  ENZYME AND MICROBIAL TECHNOLOGY, Jul. 2011, Peer-reviewed

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• Chemiluminescent Enzyme Immunoassay for Measuring Leptin

  Satoshi Sekiguchi; Hideki Kohno; Kiyoshi Yasukawa; Kuniyo Inouye

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Apr. 2011, Peer-reviewed

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• Effects of Site-Directed Mutagenesis of the Loop Residue of the N-Terminal Domain Gly117 of Thermolysin on Its Catalytic Activity

  Evans Menach; Kiyoshi Yasukawa; Kuniyo Inouye

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Dec. 2010, Peer-reviewed

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• Increase in thermal stability of Moloney murine leukaemia virus reverse transcriptase by site-directed mutagenesis

  Kiyoshi Yasukawa; Masaki Mizuno; Atsushi Konishi; Kuniyo Inouye

  JOURNAL OF BIOTECHNOLOGY, Nov. 2010, Peer-reviewed

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• Effects of Organic Solvents on the Reverse Transcription Reaction Catalyzed by Reverse Transcriptases from Avian Myeloblastosis Virus and Moloney Murine Leukemia Virus

  Kiyoshi Yasukawa; Atsushi Konishi; Kuniyo Inouye

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Sep. 2010, Peer-reviewed

  
• Effects of the mutational combinations on the activity and stability of thermolysin

  Masayuki Kusano; Kiyoshi Yasukawa; Kuniyo Inouye

  JOURNAL OF BIOTECHNOLOGY, May 2010, Peer-reviewed

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• Detection of cesA mRNA from Bacillus cereus by RNA-specific amplification

  Kiyoshi Yasukawa; Norio Agata; Kuniyo Inouye

  ENZYME AND MICROBIAL TECHNOLOGY, Apr. 2010, Peer-reviewed

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• Synthesis of N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester catalyzed by thermolysin variants with improved activity

  Masayuki Kusano; Kiyoshi Yasukawa; Kuniyo Inouye

  ENZYME AND MICROBIAL TECHNOLOGY, Mar. 2010, Peer-reviewed

  
• Insight into the Mechanism of the Stabilization of Moloney Murine Leukaemia Virus Reverse Transcriptase by Eliminating RNase H Activity

  Masaki Mizuno; Kiyoshi Yasukawa; Kuniyo Inouye

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Feb. 2010, Peer-reviewed

  
• Effects of Thermal Treatment on the Coagulation of Soy Proteins Induced by Subtilisin Carlsberg (vol 57, pg 717, 2009)

  Kuniyo Inouye; Mikio Nakano; Kohei Asaoka; Kiyoshi Yasukawa

  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, Dec. 2009, Peer-reviewed

  
• Two Crystal Structures of Lysyl-tRNA Synthetase from Bacillus stearothermophilus in Complex with Lysyladenylate-Like Compounds: Insights into the Irreversible Formation of the Enzyme-Bound Adenylate of l-Lysine Hydroxamate

  Haruko Sakurama; Teisuke Takita; Bunzo Mikami; Takafumi Itoh; Kiyoshi Yasukawa; Kuniyo Inouye

  JOURNAL OF BIOCHEMISTRY, May 2009, Peer-reviewed

  
• Coagulation of soy proteins induced by thermolysin and comparison of the coagulation reaction with that induced by subtilisin Carlsberg

  Kohei Asaoka; Kiyoshi Yasukawa; Kuniyo Inouye

  ENZYME AND MICROBIAL TECHNOLOGY, Apr. 2009, Peer-reviewed

  
• Characterization of Moloney Murine Leukaemia Virus/Avian Myeloblastosis Virus Chimeric Reverse Transcriptases

  Kiyoshi Yasukawa; Masaki Mizuno; Kuniyo Inouye

  JOURNAL OF BIOCHEMISTRY, Mar. 2009, Peer-reviewed

  
• Effects of Thermal Treatment on the Coagulation of Soy Proteins Induced by Subtilisin Carlsberg

  Kuniyo Inouye; Kimio Nakano; Kohei Asaoka; Kiyoshi Yasukawa

  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, Jan. 2009, Peer-reviewed

  
• Insights into the Catalytic Roles of the Polypeptide Regions in the Active Site of Thermolysin and Generation of the Thermolysin Variants with High Activity and Stability

  Masayuki Kusano; Kiyoshi Yasukawa; Kuniyo Inouye

  JOURNAL OF BIOCHEMISTRY, Jan. 2009, Peer-reviewed

  
• Effects of introducing negative charges into the molecular surface of thermolysin by site-directed mutagenesis on its activity and stability

  Teisuke Takita; Takahiro Aono; Haruko Sakurama; Takafumi Itoh; Takumi Wada; Masashi Minoda; Kiyoshi Yasukawa; Kuniyo Inouye

  BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Mar. 2008, Peer-reviewed

  
• Comparison of the thermal stabilities of reverse transcriptases from avian myeloblastosis virus and Moloney murine leukaemia virus

  Kiyoshi Yasukawa; Daisuke Nemoto; Kuniyo Inouye

  JOURNAL OF BIOCHEMISTRY, Feb. 2008, Peer-reviewed

  
• Improving the activity and stability of thermolysin by site-directed mutagenesis

  Kiyoshi Yasukawa; Kuniyo Inouye

  BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, Oct. 2007, Peer-reviewed

  
• Effects of neutral salts and alcohols on the activity of Streptomyces caespitosus neutral protease

  Kuniyo Inouye; Takashi Shimada; Kiyoshi Yasukawa

  JOURNAL OF BIOCHEMISTRY, Sep. 2007, Peer-reviewed

  
• A new method for the extracellular production of recombinant thermolysin by co-expressing the mature sequence and pro-sequence in Escherichia coli

  Kiyoshi Yasukawa; Masayuki Kusano; Kuniyo Inouye

  PROTEIN ENGINEERING DESIGN & SELECTION, Aug. 2007, Peer-reviewed

  
• Improvement in performance of affinity gels containing gly-D-phe as a ligand to thermolysin due to increasing the Spacer chain length

  Kuniyo Inouye; Koji Nakamura; Masayuki Kusano; Kiyoshi Yasukawa

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Aug. 2007, Peer-reviewed

  
• Purification to homogeneity of a neutral metalloproteinase from Streptomyces caespitosus

  Kuniyo Inouye; Takashi Shimada; Kiyoshi Yasukawa

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Jul. 2007, Peer-reviewed

  
• Effects of site-directed mutagenesis of the surface residues Gln128 and Gln225 of thermolysin on its catalytic activity

  Chika Tatsumi; Yasuhiko Hashida; Kiyoshi Yasukawa; Kuniyo Inouye

  JOURNAL OF BIOCHEMISTRY, Jun. 2007, Peer-reviewed

  
• Engineering, expression, purification, and production of recombinant thermolysin

  Kuniyo Inouye; Masayuki Kusano; Yasuhiko Hashida; Masashi Minoda; Kiyoshi Yasukawa

  Biotechnology Annual Review, 2007, Peer-reviewed

  
• A novel direct competitive repopulation assay for human hematopoietic stem cells using NOD/SCID mice

  T. Tatekawa; H. Ogawa; M. Kawakami; Y. Oka; K. Yasukawa; H. Sugiyama; I. Kawase; T. Soma

  CYTOTHERAPY, Aug. 2006, Peer-reviewed

  
• Engineering of the pH-dependence of thermolysin activity as examined by site-directed mutagenesis of Asn112 located at the active site of thermolysin

  Masayuki Kusano; Kiyoshi Yasukawa; Yasuhiko Hashida; Kuniyo Inouye

  JOURNAL OF BIOCHEMISTRY, Jun. 2006, Peer-reviewed

  
• Characterization of Gly-D-Phe, Gly-L-Leu, and D-Phe as affinity ligands to thermolysin

  K Yasukawa; M Kusano; K Nakamura; K Inouye

  PROTEIN EXPRESSION AND PURIFICATION, Apr. 2006, Peer-reviewed

  
• Extracellular production of recombinant thermolysin expressed in Escherichia coli, and its purification and enzymatic characterization

  K Inouye; M Minoda; T Takita; H Sakurama; Y Hashida; M Kusano; K Yasukawa

  PROTEIN EXPRESSION AND PURIFICATION, Apr. 2006, Peer-reviewed

  
• Rapid detection of Mycobacterium tuberculosis in respiratory samples by transcription-reverse transcription concerted reaction with an automated system

  S Takakura; S Tsuchiya; Y Isawa; K Yasukawa; T Hayashi; M Tomita; K Suzuki; T Hasegawa; T Tagami; A Kurashima; S Ichiyama

  JOURNAL OF CLINICAL MICROBIOLOGY, Nov. 2005, Peer-reviewed

  
• Isothermal RNA sequence amplification method for rapid antituberculosis drug susceptibility testing of Mycobacterium tuberculosis

  S Takakura; S Tsuchiya; N Fujihara; T Kudo; Y Iinuma; S Mitarai; S Ichiyama; K Yasukawa; T Ishiguro

  JOURNAL OF CLINICAL MICROBIOLOGY, May 2005, Peer-reviewed

  
• Rapid detection of tdh and trh mRNAs of Vibrio parahaemolyticus by the transcription-reverse transcription concerted (TRC) method

  N Masuda; K Yasukawa; Y Isawa; R Horie; J Saitoh; T Ishiguro; Y Nakaguchi; M Nishibuchi; T Hayashi

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, Oct. 2004, Peer-reviewed

  
• Rapid and specific detection of tdh, trh1, and trh2 mRNA of Vibrio parahaemolyticus by transcription-reverse transcription concerted reaction with an automated system

  Y Nakaguchi; T Ishizuka; S Ohnaka; T Hayashi; K Yasukawa; T Ishiguro; M Nishibuchi

  JOURNAL OF CLINICAL MICROBIOLOGY, Sep. 2004, Peer-reviewed

  
• Fusion protein of interleukin-6 and interleukin-6 receptor without a polypeptide linker

  K Yasukawa; S Tsuchiya; T Ekida; H Iida; T Ide; D Miki; H Yagame; K Murayama; T Ishiguroi

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, Jul. 2003, Peer-reviewed

  
• Intercalation activating fluorescence DNA probe and its application to homogeneous quantification of a target sequence by isothermal sequence amplification in a closed vessel

  T Ishiguro; J Saitoh; R Horie; T Hayashi; T Ishizuka; S Tsuchiya; K Yasukawa; T Kido; Y Nakaguchi; M Nishibuchi; K Ueda

  ANALYTICAL BIOCHEMISTRY, Mar. 2003, Peer-reviewed

  
• Directly linked soluble IL-6 receptor-IL-6 fusion protein induces astrocyte differentiation from neuroepithelial cells via activation of STAT3

  T Takizawa; M Yanagisawa; W Ochiai; K Yasukawa; T Ishiguro; K Nakashima; T Taga

  CYTOKINE, Mar. 2001, Peer-reviewed

  
• Enhanced signal transduction by a directly fused protein of interleukin-6 and its receptor

  H Mizuguchi; H Mizuno; K Yasukawa; T Ishiguro; K Fukui; T Imanaka; M Takagi

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, Mar. 2001, Peer-reviewed

  
• Interleukin 6 receptor expression by human cord blood- or peripheral blood-derived primitive haematopoietic progenitors implies acquisition of different functional properties

  H Minamiguchi; N Yahata; T Kimura; H Fujiki; S Harada; JF Wang; K Okuda; H Kaneko; K Hodohara; T Banba; K Yasukawa; JH Ohyashiki; K Ohyashiki; T Abe; Y Sonoda

  BRITISH JOURNAL OF HAEMATOLOGY, Aug. 2000, Peer-reviewed

  
• Screening of an oligopeptide antagonist for interleukin-6 from a random phage library

  H Mizuguchi; T Kubomi; R Nomura; K Yasukawa; T Imanaka; M Takagi

  BIOTECHNOLOGY LETTERS, Jun. 2000, Peer-reviewed

  
• Activity of interleukin 6 in the differentiation of monocytes to macrophages and dendritic cells

  H Mitani; N Katayama; H Araki; K Ohishi; K Kobayashi; H Suzuki; K Nishii; M Masuya; K Yasukawa; N Minami; H Shiku

  BRITISH JOURNAL OF HAEMATOLOGY, May 2000, Peer-reviewed

  
• Expansion of human NOD/SCID-repopulating cells by stem cell factor, Flk2/Flt3 ligand, thrombopoietin, IL-6, and soluble IL-6 receptor

  T Ueda; K Tsuji; H Yoshino; Y Ebihara; H Yagasaki; H Hisakawa; T Mitsui; A Manabe; R Tanaka; K Kobayashi; M Ito; K Yasukawa; T Nakahata

  JOURNAL OF CLINICAL INVESTIGATION, Apr. 2000, Peer-reviewed

  
• Signal through gp130 activated by soluble interleukin (IL)-6 receptor (R) and IL-6 or IL-6R/IL-6 fusion protein enhances ex vivo expansion of human peripheral blood-derived hematopoietic progenitors

  T Kimura; JF Wang; H Minamiguchi; H Fujiki; S Harada; K Okuda; H Kaneko; S Yokota; K Yasukawa; T Abe; Y Sonoda

  STEM CELLS, 2000, Peer-reviewed

  
• Soluble interleukin-6 (IL-6) receptor with IL-6 stimulates megakaryopoiesis from human CD34(+) cells through glycoprotein (gp)130 signaling

  XW Sui; K Tsuji; Y Ebihara; R Tanaka; K Muraoka; M Yoshida; K Yamada; K Yasukawa; T Taga; T Kishimoto; T Nakahata

  BLOOD, Apr. 1999, Peer-reviewed

  
• Discrimination of liver cirrhosis from chronic hepatitis by measuring the ratio of Aleuria aurantia lectin-reactive serum cholinesterase to immunoreactive protein

  T Hada; M Kondo; K Yasukawa; Y Amuro; K Higashino

  CLINICA CHIMICA ACTA, Mar. 1999, Peer-reviewed

  
• Influence of psychological stress on immune-inflammatory variables in normal humans. Part II. Altered serum concentrations of natural anti-inflammatory agents and soluble membrane antigens of monocytes and T lymphocytes

  C Song; G Kenis; A van Gastel; E Bosmans; AH Lin; R de Jong; H Neels; S Scharpe; A Janca; K Yasukawa; M Maes

  PSYCHIATRY RESEARCH, Mar. 1999, Peer-reviewed

  
• The N-terminus of gp130 is critical for the formation of the high-affinity interleukin-6 receptor complex

  RL Moritz; LD Ward; GF Tu; LJ Fabri; H Ji; K Yasukawa; RJ Simpson

  GROWTH FACTORS, 1999, Peer-reviewed

  
• Combination of interleukin-6 and soluble interleukin-6 receptors induces differentiation and activation of JAK-STAT and MAP kinase pathways in MG-63 human osteoblastic cells

  R Nishimura; K Moriyama; K Yasukawa; GR Mundy; T Yoneda

  JOURNAL OF BONE AND MINERAL RESEARCH, May 1998, Peer-reviewed

  
• Identification and characterization of two distinct truncated forms of gp130 and a soluble form of leukemia inhibitory factor receptor alpha-chain in normal human urine and plasma

  JG Zhang; Y Zhang; CM Owczarek; LD Ward; RL Moritz; RJ Simpson; K Yasukawa; NA Nicola

  JOURNAL OF BIOLOGICAL CHEMISTRY, Apr. 1998, Peer-reviewed

  
• Effect of monoclonal anti-human GP130 antibody (GPX7) on bone turnover in normal and ovariectomized rats

  S Nishida; N Okimoto; Y Okazaki; A Yamaguchi; M Kumegawa; K Yasukawa; K Murayama; T Nakamura

  CALCIFIED TISSUE INTERNATIONAL, Mar. 1998, Peer-reviewed

  
• Simultaneous activation of signals through gp130, c-kit, and interleukin-3 receptor promotes a trilineage blood cell production in the absence of terminally acting lineage-specific factors

  T Kimura; H Sakabe; S Tanimukai; T Abe; Y Urata; K Yasukawa; A Okano; T Taga; H Sugiyama; T Kishimoto; Y Sonoda

  BLOOD, Dec. 1997, Peer-reviewed

  
• Inhibition of interleukin-6-induced human immunodeficiency virus type 1 expression by anti-gp130 monoclonal antibody

  M Okamoto; K Yasukawa; K Katsuura; M Baba

  BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, Nov. 1997, Peer-reviewed

  
• Characterization of soluble gp130 released by melanoma cell lines: A polyvalent antagonist of cytokines from the interleukin 6 family

  FA MonteroJulian; H Brailly; C Sautes; Joyeux, I; T Dorval; Mosseri, V; K Yasukawa; J Wijdenes; A Adler; Gorin, I; WH Fridman; E Tartour

  CLINICAL CANCER RESEARCH, Aug. 1997, Peer-reviewed

  
• Elevated levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) activities in the sera and milk of cows with naturally occurring coliform mastitis

  Y Nakajima; O Mikami; M Yoshioka; Y Motoi; T Ito; Y Ishikawa; M Fuse; K Nakano; K Yasukawa

  RESEARCH IN VETERINARY SCIENCE, May 1997, Peer-reviewed

  
• Expression of recombinant human glutamic acid decarboxylase (GAD) in myeloma cells and enzyme-linked immunosorbent assay (ELISA) for autoantibodies to GAD

  T Matsuba; M Yano; N Abiru; H Takino; S Akazawa; S Nagataki; K Yasukawa

  JOURNAL OF BIOCHEMISTRY, Jan. 1997, Peer-reviewed

  
• Yeast expression of the cytokine receptor domain of the soluble interleukin-6 receptor

  P Vollmer; M Peters; M Ehlers; H Yagame; T Matsuba; M Kondo; K Yasukawa; KHM zumBuschenfelde; S RoseJohn

  JOURNAL OF IMMUNOLOGICAL METHODS, Nov. 1996, Peer-reviewed

  
• Genetically engineered antibodies which have reduced binding affinity for thyroxine but retain the same affinity for tri-iodothyronine

  T Taya; K Yasukawa; K Miyata; S Kidokoro; K Inouye

  BIOTECHNOLOGY LETTERS, Nov. 1996, Peer-reviewed

  
• Analysis of interleukin 6 receptor and gp130 expressions and proliferative capability of human CD34(+) cells

  S Tajima; K Tsuji; Y Ebihara; XW Sui; R Tanaka; K Muraoka; M Yoshida; K Yamada; K Yasukawa; T Taga; T Kishimoto; T Nakahata

  JOURNAL OF EXPERIMENTAL MEDICINE, Oct. 1996, Peer-reviewed

  
• Expression of cathepsin E in pancreas: A possible tumor marker for pancreas, a preliminary report

  T Azuma; M Hirai; S Ito; K Yamamoto; RT Taggart; T Matsuba; K Yasukawa; K Uno; T Hayakumo; M Nakajima

  INTERNATIONAL JOURNAL OF CANCER, Aug. 1996, Peer-reviewed

  
• Use of a surface plasmon resonance biosensor for the determination of binding constants of transiently expressed recombinant antibody to its antigen

  M Kondo; K Taya; T Matsuba; N Fushimi; K Inouye; S Kidokoro; K Yasukawa

  BIOTECHNOLOGY TECHNIQUES, Aug. 1996, Peer-reviewed

  
• Coadministration of interleukin-6 (IL-6) and soluble IL-6 receptor delays progression of wobbler mouse motor neuron disease

  K Ikeda; M Kinoshita; N Tagaya; T Shiojima; T Taga; K Yasukawa; H Suzuki; A Okano

  BRAIN RESEARCH, Jul. 1996, Peer-reviewed

  
• Differential interleukin-6 (IL-6) responses of three established myeloma cell lines in the presence of soluble human IL(-)6 receptors

  M Diamant; MB Hansen; K Rieneck; M Svenson; K Yasukawa; K Bendtzen

  LEUKEMIA RESEARCH, Apr. 1996, Peer-reviewed

  
• Erythropoietin-independent erythrocyte production: Signals through gp130 and c-kit dramatically promote erythropoiesis from human CD34(+) cells

  XW Sui; K Tsuji; S Tajima; R Tanaka; K Muraoka; Y Ebihara; K Ikebuchi; K Yasukawa; T Taga; T Kishimoto; T Nakahata

  JOURNAL OF EXPERIMENTAL MEDICINE, Mar. 1996, Peer-reviewed

  
• Enzyme-linked immunosorbent assay (ELISA) for Aleuria aurantia lectin-reactive serum cholinesterase to differentiate liver cirrhosis and chronic hepatitis

  M Kondo; T Hada; K Fukui; A Iwasaki; K Higashino; K Yasukawa

  CLINICA CHIMICA ACTA, Dec. 1995, Peer-reviewed

  
• LEUKEMIA INHIBITORY FACTOR, LIF RECEPTOR, AND GP130 IN THE MOUSE UTERUS DURING EARLY-PREGNANCY

  ZM YANG; SP LE; DB CHEN; J COTA; SIERO, V; K YASUKAWA; MJK HARPER

  MOLECULAR REPRODUCTION AND DEVELOPMENT, Dec. 1995, Peer-reviewed

  
• EXPRESSION OF RECOMBINANT HUMAN THYROTROPIN RECEPTOR IN MYELOMA CELLS

  T MATSUBA; M YAMADA; H SUZUKI; A KANAI; O ISOZAKI; T YOSHIDA; T TSUSHIMA; K YASUKAWA

  JOURNAL OF BIOCHEMISTRY, Aug. 1995, Peer-reviewed

  
• FUNCTIONAL DISTINCTION OF 2 REGIONS OF HUMAN INTERLEUKIN-6 IMPORTANT FOR SIGNAL-TRANSDUCTION VIA GP130

  FD DEHON; E TENBOEKEL; J HERRMAN; C CLEMENT; M EHLERS; T TAGA; K YASUKAWA; Y OHSUGI; T KISHIMOTO; S ROSEJOHN; J WIJDENES; R KASTELEIN; LA AARDEN; JPJ BRAKENHOFF

  CYTOKINE, Jul. 1995, Peer-reviewed

  
• A MAJOR ROLE FOR THE PROTEIN-TYROSINE KINASE JAK1 IN THE JAK/STAT SIGNAL-TRANSDUCTION PATHWAY IN RESPONSE TO INTERLEUKIN-6

  D GUSCHIN; N ROGERS; J BRISCOE; B WITTHUHN; D WATLING; F HORN; S PELLEGRINI; K YASUKAWA; P HEINRICH; GR STARK; JN IHLE; IM KERR

  EMBO JOURNAL, Apr. 1995, Peer-reviewed

  
• USE OF A BIOSENSOR WITH SURFACE-PLASMON RESONANCE DETECTION FOR THE DETERMINATION OF BINDING CONSTANTS - MEASUREMENT OF INTERLEUKIN-6 BINDING TO THE SOLUBLE INTERLEUKIN-6 RECEPTOR

  LD WARD; GJ HOWLETT; A HAMMACHER; J WEINSTOCK; K YASUKAWA; RJ SIMPSON; DJ WINZOR

  BIOCHEMISTRY, Mar. 1995, Peer-reviewed

  
• INFLUENCE OF INTERLEUKIN-6 (IL-6) AUTOANTIBODIES ON IL-6 BINDING TO CELLULAR RECEPTORS

  MB HANSEN; M SVENSON; K ABELL; K YASUKAWA; M DIAMANT; K BENDTZEN

  EUROPEAN JOURNAL OF IMMUNOLOGY, Feb. 1995, Peer-reviewed

  
• A NEW SYSTEM FOR THE EXPRESSION OF RECOMBINANT ANTIBODY IN MAMMALIAN-CELLS

  Y IBA; T KANEKO; T EKIDA; K MIYATA; K INOUYE; Y KUROSAWA; K YASUKAWA

  BIOTECHNOLOGY LETTERS, Feb. 1995, Peer-reviewed

  
• Studies of cytokine cytokine receptor interactions: Influence of ligand dimerization

  LD WARD; GJ HOWLETT; RL MORITZ; A HAMMACHER; K YASUKAWA; RJ SIMPSON

  TECHNIQUES IN PROTEIN CHEMISTRY VI, 1995, Peer-reviewed

  
• EXPRESSION OF RECOMBINANT ANTIBODY AGAINST CANCER-SPECIFIC CARBOHYDRATE

  K TAKAGI; K YASUKAWA; Y IBA; Y ISOBE; Y SUKETA; R KANNAGI; K KOHDA; M TAKAGI; T IMANAKA

  JOURNAL OF FERMENTATION AND BIOENGINEERING, 1995, Peer-reviewed

  
• STRUCTURE-FUNCTION ANALYSIS OF HUMAN IL-6 - IDENTIFICATION OF 2 DISTINCT REGIONS THAT ARE IMPORTANT FOR RECEPTOR-BINDING

  A HAMMACHER; LD WARD; J WEINSTOCK; H TREUTLEIN; K YASUKAWA; RJ SIMPSON

  PROTEIN SCIENCE, Dec. 1994, Peer-reviewed

  
• HIGH-AFFINITY INTERLEUKIN-6 RECEPTOR IS A HEXAMERIC COMPLEX CONSISTING OF 2 MOLECULES EACH OF INTERLEUKIN-6, INTERLEUKIN-6 RECEPTOR, AND GP-130

  LD WARD; GJ HOWLETT; G DISCOLO; K YASUKAWA; A HAMMACHER; RL MORITZ; RJ SIMPSON

  JOURNAL OF BIOLOGICAL CHEMISTRY, Sep. 1994, Peer-reviewed

  
• STIMULATION OF THE B9 HYBRIDOMA CELL-LINE BY SOLUBLE INTERLEUKIN-6 RECEPTORS

  M DIAMANT; MB HANSEN; K RIENECK; M SVENSON; K YASUKAWA; K BENDTZEN

  JOURNAL OF IMMUNOLOGICAL METHODS, Aug. 1994, Peer-reviewed

  
• IDENTIFICATION OF A REGION WITHIN THE CYTOPLASMIC DOMAIN OF THE INTERLEUKIN-6 (IL-6) SIGNAL TRANSDUCER GP130 IMPORTANT FOR LIGAND-INDUCED ENDOCYTOSIS OF THE IL-6 RECEPTOR

  E DITTRICH; S ROSEJOHN; C GERHARTZ; J MULLBERG; T STOYAN; K YASUKAWA; PC HEINRICH; L GRAEVE

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jul. 1994, Peer-reviewed

  
• A HIGHLY SENSITIVE QUANTITATIVE BIOASSAY FOR HUMAN INTERLEUKIN-11

  ZY LU; XG ZHANG; ZJ GU; K YASUKAWA; M AMIOT; M ETRILLARD; R BATAILLE; B KLEIN

  JOURNAL OF IMMUNOLOGICAL METHODS, Jul. 1994, Peer-reviewed

  
• BIOSYNTHESIS AND HALF-LIFE OF THE INTERLEUKIN-6 RECEPTOR AND ITS SIGNAL TRANSDUCER GP130

  C GERHARTZ; E DITTRICH; T STOYAN; S ROSEJOHN; K YASUKAWA; PC HEINRICH; L GRAEVE

  EUROPEAN JOURNAL OF BIOCHEMISTRY, Jul. 1994, Peer-reviewed

  
• IDENTIFICATION OF A 130-KILODALTON TYROSINE-PHOSPHORYLATED PROTEIN-INDUCED BY INTERLEUKIN-11 AS JAK2 TYROSINE KINASE, WHICH ASSOCIATES WITH GP130 SIGNAL TRANSDUCER

  TG YIN; K YASUKAWA; T TAGA; T KISHIMOTO; YC YANG

  EXPERIMENTAL HEMATOLOGY, May 1994, Peer-reviewed

  
• CILIARY NEUROTROPIC FACTOR, INTERLEUKIN-11, LEUKEMIA INHIBITORY FACTOR, AND ONCOSTATIN-M ARE GROWTH-FACTORS FOR HUMAN MYELOMA CELL-LINES USING THE INTERLEUKIN-6 SIGNAL TRANSDUCER GP130

  XG ZHANG; JJ GU; ZY LU; K YASUKAWA; GD YANCOPOULOS; K TURNER; M SHOYAB; T TAGA; T KISHIMOTO; R BATAILLE; B KLEIN

  JOURNAL OF EXPERIMENTAL MEDICINE, Apr. 1994, Peer-reviewed

  
• NITRIC-OXIDE BLOCKS THE CELL-CYCLE OF MOUSE MACROPHAGE-LIKE CELLS IN THE EARLY G(2)+M PHASE

  K TAKAGI; Y ISOBE; K YASUKAWA; E OKOUCHI; Y SUKETA

  FEBS LETTERS, Mar. 1994, Peer-reviewed

  
• MAINTENANCE OF THE PLURIPOTENTIAL PHENOTYPE OF EMBRYONIC STEM-CELLS THROUGH DIRECT ACTIVATION OF GP130 SIGNALING PATHWAYS

  K YOSHIDA; CHAMBERS, I; J NICHOLS; A SMITH; M SAITO; K YASUKAWA; M SHOYAB; T TAGA; T KISHIMOTO

  MECHANISMS OF DEVELOPMENT, Feb. 1994, Peer-reviewed

  
• ASSOCIATION OF TRANSCRIPTION FACTOR APRF AND PROTEIN-KINASE JAK1 WITH THE INTERLEUKIN-6 SIGNAL TRANSDUCER GP130

  C LUTTICKEN; UM WEGENKA; JP YUAN; J BUSCHMANN; C SCHINDLER; A ZIEMIECKI; AG HARPUR; AF WILKS; K YASUKAWA; T TAGA; T KISHIMOTO; G BARBIERI; S PELLEGRINI; M SENDTNER; PC HEINRICH; F HORN

  SCIENCE, Jan. 1994, Peer-reviewed

  
• RECONSTITUTION IN-VITRO OF THE INTERLEUKIN-6 INTERLEUKIN-6 RECEPTOR INTERACTION - DIRECT MONITORING USING A BIOSENSOR EMPLOYING SURFACE-PLASMON RESONANCE DETECTION

  LD WARD; A HAMMACHER; J CHANG; JG ZHANG; G DISCOLO; RL MORITZ; K YASUKAWA; RJ SIMPSON

  TECHNIQUES IN PROTEIN CHEMISTRY V, 1994, Peer-reviewed

  
• DIFFERENTIAL SHEDDING OF THE 2 SUBUNITS OF THE INTERLEUKIN-6 RECEPTOR

  J MULLBERG; E DITTRICH; L GRAEVE; C GERHARTZ; K YASUKAWA; T TAGA; T KISHIMOTO; PC HEINRICH; S ROSEJOHN

  FEBS LETTERS, Oct. 1993, Peer-reviewed

  
• INVOLVEMENT OF IL-6 SIGNAL TRANSDUCER GP130 IN IL-11-MEDIATED SIGNAL-TRANSDUCTION

  TG YIN; T TAGA; MLS TSANG; K YASUKAWA; T KISHIMOTO; YC YANG

  JOURNAL OF IMMUNOLOGY, Sep. 1993, Peer-reviewed

  
• ROLE OF THE C-TERMINUS IN THE ACTIVITY, CONFORMATION, AND STABILITY OF INTERLEUKIN-6

  LD WARD; A HAMMACHER; JG ZHANG; J WEINSTOCK; K YASUKAWA; CJ MORTON; RS NORTON; RJ SIMPSON

  PROTEIN SCIENCE, Sep. 1993, Peer-reviewed

  
• SOLUBLE FORMS OF THE INTERLEUKIN-6 SIGNAL-TRANSDUCING RECEPTOR COMPONENT GP130 IN HUMAN SERUM POSSESSING A POTENTIAL TO INHIBIT SIGNALS THROUGH MEMBRANE-ANCHORED GP130

  M NARAZAKI; K YASUKAWA; T SAITO; Y OHSUGI; H FUKUI; Y KOISHIHARA; GD YANCOPOULOS; T TAGA; T KISHIMOTO

  BLOOD, Aug. 1993, Peer-reviewed

  
• PREPARATION OF MONOCLONAL-ANTIBODIES AGAINST THE IL-6 SIGNAL TRANSDUCER, GP130, THAT CAN INHIBIT IL-6-MEDIATED FUNCTIONS

  T SAITO; T TAGA; D MIKI; K FUTATSUGI; H YAWATA; T KISHIMOTO; K YASUKAWA

  JOURNAL OF IMMUNOLOGICAL METHODS, Aug. 1993, Peer-reviewed

  
• IL-6-INDUCED HOMODIMERIZATION OF GP-130 AND ASSOCIATED ACTIVATION OF A TYROSINE KINASE

  M MURAKAMI; M HIBI; N NAKAGAWA; T NAKAGAWA; K YASUKAWA; K YAMANISHI; T TAGA; T KISHIMOTO

  SCIENCE, Jun. 1993, Peer-reviewed

  
• SERUM-SOLUBLE INTERLEUKIN-6 RECEPTOR IN MRL/LPR MISE IS ELEVATED WITH AGE AND MEDIATES THE INTERLEUKIN-6 SIGNAL

  H SUZUKI; K YASUKAWA; T SAITO; M NARAZAKI; A HASEGAWA; T TAGA; T KISHIMOTO

  EUROPEAN JOURNAL OF IMMUNOLOGY, May 1993, Peer-reviewed

  
• INCREASED AND HIGHLY STABLE LEVELS OF FUNCTIONAL SOLUBLE INTERLEUKIN-6 RECEPTOR IN SERA OF PATIENTS WITH MONOCLONAL GAMMOPATHY

  JP GAILLARD; R BATAILLE; H BRAILLY; C ZUBER; K YASUKAWA; M ATTAL; N MARUO; T TAGA; T KISHIMOTO; B KLEIN

  EUROPEAN JOURNAL OF IMMUNOLOGY, Apr. 1993, Peer-reviewed

  
• STRUCTURE-FUNCTION ANALYSIS OF HUMAN IL-6 RECEPTOR - DISSOCIATION OF AMINO-ACID-RESIDUES REQUIRED FOR IL-6-BINDING AND FOR IL-6 SIGNAL TRANSDUCTION THROUGH GP130

  H YAWATA; K YASUKAWA; S NATSUKA; M MURAKAMI; K YAMASAKI; M HIBI; T TAGA; T KISHIMOTO

  EMBO JOURNAL, Apr. 1993, Peer-reviewed

  
• PREPARATION OF MOUSE-HUMAN CHIMERIC ANTIBODY TO AN EMBRYONIC CARBOHYDRATE ANTIGEN, LEWIS-Y

  T KANEKO; Y IBA; K ZENITA; K SHIGETA; K NAKANO; W ITOH; Y KUROSAWA; R KANNAGI; K YASUKAWA

  JOURNAL OF BIOCHEMISTRY, Jan. 1993, Peer-reviewed

  
• INDUCTION OF ACUTE PHASE PROTEIN BY RECOMBINANT HUMAN INTERLEUKIN-6 (IL-6) IN CALVES

  Y NAKAJIMA; E MOMOTANI; T MURAKAMI; Y ISHIKAWA; M MORIMATSU; M SAITO; H SUZUKI; K YASUKAWA

  VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, Jan. 1993, Peer-reviewed

  
• ABNORMAL DISTRIBUTION OF IL-6 RECEPTOR IN AGED MRL/LPR MICE - ELEVATED EXPRESSION ON B-CELLS AND ABSENCE ON CD4+ CELLS

  KOBAYASHI, I; T MATSUDA; T SAITO; K YASUKAWA; H KIKUTANI; T HIRANO; T TAGA; T KISHIMOTO

  INTERNATIONAL IMMUNOLOGY, Dec. 1992, Peer-reviewed

  
• DOWN-REGULATION OF INTERLEUKIN-6 RECEPTORS OF MOUSE MYELOMONOCYTIC LEUKEMIC-CELLS BY LEUKEMIA INHIBITORY FACTOR

  M YAMAGUCHI; M MICHISHITA; K HIRAYOSHI; K YASUKAWA; M OKUMA; K NAGATA

  JOURNAL OF BIOLOGICAL CHEMISTRY, Nov. 1992, Peer-reviewed

  
• ROLE OF LEUCINE RESIDUES IN THE C-TERMINAL REGION OF HUMAN INTERLEUKIN-6 IN THE BIOLOGICAL-ACTIVITY

  C NISHIMURA; T EKIDA; K NOMURA; K SAKAMOTO; H SUZUKI; K YASUKAWA; T KISHIMOTO; Y ARATA

  FEBS LETTERS, Oct. 1992, Peer-reviewed

  
• INTERLEUKIN-6 SIGNAL TRANSDUCER GP130 MEDIATES ONCOSTATIN-M SIGNALING

  JW LIU; B MODRELL; A ARUFFO; JS MARKEN; T TAGA; K YASUKAWA; M MURAKAMI; T KISHIMOTO; M SHOYAB

  JOURNAL OF BIOLOGICAL CHEMISTRY, Aug. 1992, Peer-reviewed

  
• ANTI-HUMAN INTERLEUKIN-6 RECEPTOR ANTIBODY INHIBITS HUMAN MYELOMA GROWTH-INVIVO

  H SUZUKI; K YASUKAWA; T SAITO; R GOITSUKA; A HASEGAWA; Y OHSUGI; T TAGA; T KISHIMOTO

  EUROPEAN JOURNAL OF IMMUNOLOGY, Aug. 1992, Peer-reviewed

  
• SEPARATION OF NEUTRAL GLYCOASPARAGINES ACCORDING TO THEIR CONTENT OF CIS DIOL GROUPS

  H NOMOTO; K YASUKAWA; Y INOUE

  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Jul. 1992, Peer-reviewed

  
• AN ESTABLISHMENT OF ELISA FOR MURINE IL-6

  Takashi Saito; Kensuke Futatsugi; Daisuke Miki; Hiroshi Suzuki; Kiyoshi Yasukawa

  BIOTECHNOLOGY TECHNIQUES, Jul. 1992, Peer-reviewed

  
• CNTF AND LIF ACT ON NEURONAL CELLS VIA SHARED SIGNALING PATHWAYS THAT INVOLVE THE IL-6 SIGNAL TRANSDUCING RECEPTOR COMPONENT GP130

  NY IP; SH NYE; TG BOULTON; S DAVIS; T TAGA; YP LI; SJ BIRREN; K YASUKAWA; T KISHIMOTO; DJ ANDERSON; N STAHL; GD YANCOPOULOS

  CELL, Jun. 1992, Peer-reviewed

  
• HUMAN SOLUBLE IL-6 RECEPTOR - ITS DETECTION AND ENHANCED RELEASE BY HIV-INFECTION

  M HONDA; S YAMAMOTO; M CHENG; K YASUKAWA; H SUZUKI; T SAITO; Y OSUGI; T TOKUNAGA; T KISHIMOTO

  JOURNAL OF IMMUNOLOGY, Apr. 1992, Peer-reviewed

  
• SOLUBLE INTERLEUKIN-6 RECEPTOR IS RELEASED FROM RECEPTOR-BEARING CELL-LINES INVITRO

  T NAKAJIMA; S YAMAMOTO; M CHENG; K YASUKAWA; T HIRANO; T KISHIMOTO; T TOKUNAGA; M HONDA

  JAPANESE JOURNAL OF CANCER RESEARCH, Apr. 1992, Peer-reviewed

  
• ASSOCIATION OF RECOMBINANT SOLUBLE IL-6-SIGNAL TRANSDUCER, GP130, WITH A COMPLEX OF IL-6-RECEPTOR AND SOLUBLE IL-6-RECEPTOR, AND ESTABLISHMENT OF AN ELISA FOR SOLUBLE GP130

  K YASUKAWA; K FUTATSUGI; T SAITO; H YAWATA; M NARAZAKI; H SUZUKI; T TAGA; T KISHIMOTO

  IMMUNOLOGY LETTERS, Feb. 1992, Peer-reviewed

  
• INTERLEUKIN-6 RECEPTOR AND SIGNALS

  T TAGA; M HIBI; M MURAKAMI; M SAITO; H YAWATA; M NARAZAKI; Y HIRATA; T SUGITA; K YASUKAWA; T HIRANO; T KISHIMOTO

  CHEMICAL IMMUNOLOGY, 1992, Peer-reviewed

  
• ANTIMURINE IL-6 RECEPTOR ANTIBODY INHIBITS IL-6 EFFECTS INVIVO

  H SUZUKI; K YASUKAWA; T SAITO; M ANZAI; R GOITSUKA; A HASEGAWA; Y OHSUGI; T TAGA; T KISHIMOTO

  IMMUNOLOGY LETTERS, Sep. 1991, Peer-reviewed

  
• MURINE ANTI-HUMAN IL-6 MONOCLONAL-ANTIBODY PROLONGS THE HALF-LIFE IN CIRCULATING BLOOD AND THUS PROLONGS THE BIOACTIVITY OF HUMAN IL-6 IN MICE

  M MIHARA; Y KOISHIHARA; H FUKUI; K YASUKAWA; Y OHSUGI

  IMMUNOLOGY, Sep. 1991, Peer-reviewed

  
• PREPARATION OF SOLUBLE MURINE IL-6 RECEPTOR AND ANTIMURINE IL-6 RECEPTOR ANTIBODIES

  T SAITO; K YASUKAWA; H SUZUKI; K FUTATSUGI; T FUKUNAGA; C YOKOMIZO; Y KOISHIHARA; H FUKUI; Y OHSUGI; H YAWATA; KOBAYASHI, I; T HIRANO; T TAGA; T KISHIMOTO

  JOURNAL OF IMMUNOLOGY, Jul. 1991, Peer-reviewed

  
• BOTH D-FACTOR LIF AND IL-6 INHIBIT THE DIFFERENTIATION OF MOUSE TERATOCARCINOMA F9 CELLS

  K HIRAYOSHI; A TSURU; M YAMASHITA; M TOMIDA; Y YAMAMOTOYAMAGUCHI; K YASUKAWA; M HOZUMI; DV GOEDDEL; K NAGATA

  FEBS LETTERS, May 1991, Peer-reviewed

  
• SITE-SPECIFIC MUTAGENESIS OF HUMAN INTERLEUKIN-6 AND ITS BIOLOGICAL-ACTIVITY

  C NISHIMURA; K FUTATSUGI; K YASUKAWA; T KISHIMOTO; Y ARATA

  FEBS LETTERS, Apr. 1991, Peer-reviewed

  
• CHEMICAL MODIFICATION AND H-1-NMR STUDIES ON THE RECEPTOR-BINDING REGION OF HUMAN INTERLEUKIN-6

  C NISHIMURA; T EKIDA; S MASUDA; K FUTATSUGI; S ITOH; K YASUKAWA; T KISHIMOTO; Y ARATA

  EUROPEAN JOURNAL OF BIOCHEMISTRY, Mar. 1991, Peer-reviewed

  
• PROTON NUCLEAR-MAGNETIC-RESONANCE STUDY OF HUMAN INTERLEUKIN-6 - CHEMICAL MODIFICATIONS AND PARTIAL SPECTRAL ASSIGNMENTS FOR THE AROMATIC RESIDUES

  C NISHIMURA; H HANZAWA; S ITOH; K YASUKAWA; SHIMADA, I; T KISHIMOTO; Y ARATA

  BIOCHIMICA ET BIOPHYSICA ACTA, Dec. 1990, Peer-reviewed

  
• PURIFICATION AND CHARACTERIZATION OF SOLUBLE HUMAN IL-6 RECEPTOR EXPRESSED IN CHO CELLS

  K YASUKAWA; T SAITO; T FUKUNAGA; Y SEKIMORI; Y KOISHIHARA; H FUKUI; Y OHSUGI; T MATSUDA; H YAWATA; T HIRANO; T TAGA; T KISHIMOTO

  JOURNAL OF BIOCHEMISTRY, Oct. 1990, Peer-reviewed

  
• A NEW SYSTEM FOR THE EXPRESSION OF HUMAN INTERLEUKIN-6 IN ESCHERICHIA-COLI

  K YASUKAWA; T SAITO

  BIOTECHNOLOGY LETTERS, Jun. 1990, Peer-reviewed

  
• IL-6 ACTIVITY IN FELINE INFECTIOUS PERITONITIS

  R GOITSUKA; T OHASHI; K ONO; K YASUKAWA; Y KOISHIBARA; H FUKUI; Y OHSUGI; A HASEGAWA

  JOURNAL OF IMMUNOLOGY, Apr. 1990, Peer-reviewed

  
• INTERLEUKIN-6 TRIGGERS THE ASSOCIATION OF ITS RECEPTOR WITH A POSSIBLE SIGNAL TRANSDUCER, GP130

  T TAGA; M HIBI; Y HIRATA; K YAMASAKI; K YASUKAWA; T MATSUDA; T HIRANO; T KISHIMOTO

  CELL, Aug. 1989, Peer-reviewed

  
• Interleukin-6 receptor and a unique mechanism of its signal transduction

  T. Taga; M. Hibi; Y. Hirata; H. Yawata; S. Natsuka; K. Yasukawa; T. Totsuka; K. Yamasaki; T. Hirano; T. Kishimoto

  Cold Spring Harbor Symposia on Quantitative Biology, 1989, Peer-reviewed

  
• Molecular Cloning of the cDNAs for Interleukin‐6/B Cell Stimulatory Factor 2 and Its Receptor

  T. HIRANO; T. TAGA; K. YAMASAKI; T. MATSUDA; K. YASUKAWA; Y. HIRATA; H. YAWATA; O. TANABE; S. AKIRA; T. KISHIMOTO

  Annals of the New York Academy of Sciences, 1989, Peer-reviewed

  
• HUMAN B-CELL STIMULATORY FACTOR-II EXPRESSED IN ESCHERICHIA-COLI

  Y ASAGOE; K YASUKAWA; T SAITO; N MARUO; K MIYATA; T KONO; T MIYAKE; T KATO; H KAKIDANI; M MITANI

  BIO-TECHNOLOGY, Jul. 1988, Peer-reviewed

  
• The production of BSF-2/IL-6 by human glioblastoma

  N. Maruo; K. Miyata; K. Yasukawa; T. Fukunaga; M. Mitani

  International Journal of Immunopharmacology, 1988, Peer-reviewed

  
• STRUCTURE AND EXPRESSION OF HUMAN B-CELL STIMULATORY FACTOR-II (BSF-2/IL-6) GENE

  K YASUKAWA; T HIRANO; Y WATANABE; K MURATANI; T MATSUDA; S NAKAI; T KISHIMOTO

  EMBO JOURNAL, Oct. 1987, Peer-reviewed

  
• COMPLEMENTARY-DNA FOR A NOVEL HUMAN INTERLEUKIN (BSF-2) THAT INDUCES LYMPHOCYTES-B TO PRODUCE IMMUNOGLOBULIN

  T HIRANO; K YASUKAWA; H HARADA; T TAGA; Y WATANABE; T MATSUDA; S KASHIWAMURA; K NAKAJIMA; K KOYAMA; A IWAMATSU; S TSUNASAWA; F SAKIYAMA; H MATSUI; Y TAKAHARA; T TANIGUCHI; T KISHIMOTO

  NATURE, Nov. 1986, Peer-reviewed

  
• THE 11.9-GHZ RAIN ATTENUATION AND SITE DIVERSITY EFFECT ON THE EARTH-SPACE PATHS OF LOW ELEVATION ANGLES

  K YASUKAWA; M YAMADA

  RADIO SCIENCE, 1985, Peer-reviewed

  
• GRAPHIC DISPLAY OF NUCLEIC-ACID STRUCTURE BY A MICROCOMPUTER

  K WATANABE; K YASUKAWA; K ISO

  NUCLEIC ACIDS RESEARCH, 1984, Peer-reviewed

  

Misc.

• Streptomyces moboraensis由来トランスグルタミナーゼの高純度成熟体の精製法の検討

  中井逸斗; 森本健太; 濱中雄大; 山田章文; 雷雨坤; 京逸如; 山形昌也; 水谷公彦; 三上文三; 三上文三; 保川清; 滝田禎亮

  生物高分子, 2023

  
• 放線菌由来P450(CYP105A1)の機能改変と医薬品代謝研究への応用:R84A変異体による非ステロイド性抗炎症薬(NSAIDs)の代謝

  鎌田成栄; 和田萌加; 山形昌也; 水谷公彦; 三上文三; 三上文三; 大橋錬; 佐久間宙; 根本翔; NILOUYAL Somaye; 余語祐哉; 安田佳織; 濱田昌弘; 榊利之; 保川清; 保川清; 滝田禎亮; 滝田禎亮

  生物高分子, 2023

  
• Crystal structure of Grimontia hollisae collagenase

  保川清; 池内健晃; 安本瑞貴; 上島聡音理; 滝田禎亮; 田中啓友; 楠畑雅; 服部俊治; 水谷公彦; 三上文三; 三上文三

  日本農芸化学会大会講演要旨集(Web), 2023

  
• トロンビンサイト導入Chryseobacterium proteolyticum由来プロテイングルタミナーゼのX線結晶構造解析

  藤井隼; 水谷公彦; 三上文三; 三上文三; 保川清; 滝田禎亮

  日本農芸化学会中部支部例会講演要旨集(Web), 2023

  
• GH10キシラナーゼXynRの315位のアミノ酸残基への変異が好アルカリ性,耐アルカリ性,耐熱性に与える影響

  中村友香; 鍬田航平; 滝田禎亮; 水谷公彦; 三上文三; 三上文三; 中村聡; 保川清

  日本農芸化学会中部支部例会講演要旨集(Web), 2023

  
• Grimontia hollisaeコラゲナーゼの触媒機構についての考察

  上島聡音理; 安本瑞貴; 北川雄登; 赤澤佳穂; 滝田禎亮; 滝田禎亮; 田中啓友; 服部俊治; 水谷公彦; 三上文三; 三上文三; 保川清; 保川清

  日本農芸化学会中部支部例会講演要旨集(Web), 2023

  
• Geobacillus stearothermophilus由来トランスグルタミナーゼ(GsTgl)のC末端伸長配列の役割の解明とX線結晶構造解析

  濱中雄大; 京逸如; 雷雨坤; 山田章文; 三上文三; 三上文三; 水谷公彦; 保川清; 滝田禎亮

  生物高分子, 2022

  
• Structural analysis of collagenase from Grimontia hollisae

  安本瑞貴; 池内健晃; 滝田禎亮; 水谷公彦; 三上文三; 三上文三; 田中啓友; 服部俊治; 保川清

  日本農芸化学会大会講演要旨集(Web), 2022

  
• Grimontia hollisaeコラゲナーゼのX線結晶構造と触媒反応から明らかになったコラーゲンに対する新規な基質特異性

  上島聡音理; 滝田禎亮; 滝田禎亮; 安本瑞貴; 池内健晃; 田中啓友; 楠畑雅; 服部俊治; 水谷公彦; 三上文三; 三上文三; 保川清; 保川清

  日本農芸化学会関西支部講演会講演要旨集, 2022

  
• Purification of subtilisin-like serine protease from Ficus carica fruit

  床並実津希; 上島直生; 西村耕作; 滝田禎亮; 保川清

  日本農芸化学会大会講演要旨集(Web), 2022

  
• Purification and characterization of serine protease from Ficus carica

  上島直生; 西村耕作; 西村耕作; 東矢圭右; 兒島憲二; 滝田禎亮; 阿部竜也; 高橋徹; 保川清

  日本農芸化学会大会講演要旨集(Web), 2021

  
• MMLV逆転写酵素のRNase Hドメインの調製と性状解析

  西村耕作; 横川貫太; 深津浩佑; 久世郁実; 久好哲郎; 小西篤; 三上文三; 兒島憲二; 保川清

  日本農芸化学会大会講演要旨集(Web), 2015

  
• 1P-031 Enhancing effect of thermostable archaeal helicase on the PCR specificity

  Fujiwara Ayako; Yasukawa Kiyoshi; Hidese Ryota; Fujiwara Shinsuke

  日本生物工学会大会講演要旨集, 2015

  
• 1A14-1 Extracellular production of recombinant thermolysin expressed in Escherichia coli, and its purification and enzymatic characterization

  YASUKAWA Kiyoshi; MINODA Masashi; TAKITA Teisuke; SAKURAMA Haruko; HASHIDA Yasuhiko; KUSANO Masayuki; INOUYE Kuniyo

  日本生物工学会大会講演要旨集, 2005

  
• 新規3酵素の組合せによるcDNA合成技術の開発 (特集 核酸の高感度検出技術とゲノム編集・合成技術の最前線)

  岡野 啓志; 保川 清

  生物工学会誌, 2017

  
• 全アミノ酸スキャニング変異導入法により得られた耐熱型GH10およびGH11キシラナーゼの性状解析

  鈴木愛美; 滝田禎亮; 中谷滉太; 李瞳陽; 片野裕太; 兒島憲二; 坂直樹; 三上文三; 八波利恵; 中村聡; 保川清

  日本農芸化学会関西支部例会（第507回講演会）, 2019

  
• Geobacillus stearothermophilus、Bacillus subtilis、Streptomyces moboraensis 由来トランスグルタミナーゼの性状解析と比較

  山田章文; 雷雨坤; 京逸如; 保川清; 滝田禎亮

  日本農芸化学会 関西支部例会（第508回講演会）, 2019

  
• 改良された次世代シーケンスによる逆転写酵素の正確性の解析

  岡野 啓志; 馬場 美聡; 秀瀬 涼太; 飯田 慶; 李 瞳陽; 兒島 憲二; 滝田 禎亮; 柳原 格; 藤原 伸介; 保川清

  日本農芸化学会2019年度大会, 2019

  
• リボヌクレアーゼH2を欠損させたマウス線維芽由来NIH3T3細胞の作製

  月足 元希; 馬場美聡; 兒島憲二; 姫田康平; 滝田禎亮; 保川清

  日本農芸化学会2019年度大会, 2019

  
• 全アミノ酸スキャニング変異導入法による耐熱型Bacillus sp. 41M-1株由来キシラナーゼの取得とその性状解析

  滝田禎亮; 中谷滉太; 片野裕太; 鈴木愛美; 兒島憲二; 坂直樹; 三上文三; 八波利恵; 中村聡; 保川清

  日本農芸化学会2019年度大会, 2019

  
• リボヌクレアーゼH2を欠損させたマウス繊維芽細胞株NIH3T3の性状解析

  姫田康平; 月足元希; 馬場美聡; 兒島憲二; 滝田禎亮; 保川 清

  第６６回近畿支部例会, 2019

  
• 続・生物工学基礎講座 : バイオよもやま話 酵素反応の最適化

  兒島 憲二; 滝田 禎亮; 保川 清

  生物工学会誌, 2018

  
• シマグワ茶成分によるα-グルコシダーゼ阻害機構の解析

  中山珠里; 伊東昌章; 兒島憲二; 滝田禎亮; 保川 清

  日本生物高分子学会2018年度大会, 2018

  
• RNA増幅法を用いたミュータンス菌の検出

  今井翔太; 多田孝清; 東隆行; 浅井莉奈; 兒島憲二; 滝田禎亮; 保川清

  日本生物高分子学会2018年度大会, 2018

  
• ジュンサイ抽出液中のヒト免疫不全ウイルス1型逆転写酵素阻害活性成分の同定

  石塚啓子; 角江亮太; 馬場美聡; 西村耕作; 畠恵司; 高橋砂織; 作田庄平; 兒島憲二; 滝田禎亮; 保川清

  日本生物高分子学会2018年度大会, 2018

  
• ジュンサイ抽出液からのヒト免疫不全ウイルス1型逆転写酵素阻害物質の精製

  石塚啓子; 角江亮太; 馬場美聡; 西村耕作; 畠恵司; 高橋砂織; 作田庄平; 兒島憲二; 滝田禎亮; 保川清

  日本農芸化学会関西支部例会（第506回講演会）, 2018

  
• エカルディーグティエール症候群（AGS）の患者で同定された変異が導入された組換えヒトRNase H2の性状解析

  西村拓人; 馬場美聡; 兒島憲二; 滝田禎亮; 保川清

  日本農芸化学会関西支部大会 (第505回講演会), 2018

  
• 全アミノ酸スキャニング変異導入法と無細胞タンパク質合成系を用いたMMLV逆転写酵素の耐熱化

  片野裕太; 李瞳陽; 馬場美聡; 中村実世; 伊東昌章; 滝田禎亮; 保川清

  日本農芸化学会関西支部大会 (第505回講演会), 2018

  
• 全アミノ酸スキャニング変異導入法によるBacillus sp. 41M-1株由来キシラナーゼの耐熱化

  中谷滉太; 片野裕太; 兒島憲二; 滝田禎亮; 八波利恵; 中村聡; 保川清

  日本農芸化学会関西支部例会第502回講演会, 2018

  
• リボヌクレアーゼH2を欠損したNIH3T3細胞株の樹立とその性状解析

  月足元希; 馬場美聡; 兒島憲二; 滝田禎亮; 保川清

  日本農芸化学会関西支部例会第503回講演会, 2018

  
• 部位特異的変異導入によるヒトリボヌクレアーゼH2の基質特異性の改変

  兒島憲二; 馬場美聡; 西村; 滝田禎亮; 上原 了; Robert; J. Crouch; 保川清

  第65回近畿支部例会, 2018

  
• リボヌクレアーゼH2をノックアウトしたNIH3T3細胞株の性状解析

  月足元希; 馬場美聡; 兒島憲二; 滝田禎亮; 保川 清

  第91回日本生化学会大会, 2018

  
• 変異の組み合わせによるモロニ―マウス白血病ウイルス逆転写酵素のさらなる耐熱化変異体の創製

  馬場美聡; 角江亮太; Christoph Leucht; Peter Rasor; Heiko Walch; Daniel Ladiges; Christian Bell; 兒島憲二; 滝田禎亮; 保川清

  第91回日本生化学会大会, 2018

  
• 逆転写活性を有する耐熱型DNAポリメラーゼによる高感度なone-step RT-PCR

  岡野啓志; 馬場美聡; 川戸克展; 秀瀬涼太; 柳原格; 兒島憲二; 滝田禎亮; 藤原伸介; 保川清

  日本農芸化学会関西支部例会第502回講演会, 2018

  
• Preparation and characterization of electron transfer proteins for efficient production of active forms of vitamin D by actinomycete CYP105A1

  Somaye Nilouyal; Teisuke Takita; Kaori Yasuda; Keiko Hayashi; Shinichi Ikushiro; Toshiyuki. Sakaki; Kiyoshi Yasukawa

  日本生物高分子学会2017年度大会, 2017

  
• MMLV逆転写酵素、耐熱型DNAポリメラーゼおよびDNA/RNAヘリカーゼの組み合わせによる高感度なone-step RT-PCR

  岡野啓志; 馬場美聡; 山崎朋美; 秀瀬涼太; 藤原伸介; 柳原格; 林司; 宇治家武史; 兒島憲二; 滝田禎亮; 保川清

  2017年度生命科学系学会合同年次大会（ConBio2017）, 2017

  
• ヒトリボヌクレアーゼ H2の基質特異性の改変

  兒島憲二; 馬場美聡; 杉浦拓也; 西村拓人; 滝田禎亮; 上原了; Robert Crouch; 保川清

  2017年度生命科学系学会合同年次大会（ConBio2017）, 2017

  
• 全アミノ酸スキャニング変異導入法によるBacillus sp. TAR-1株キシラナーゼの耐熱化

  片野裕太; 中谷滉太; 兒島憲二; 滝田禎亮; 八波利恵; 中村聡; 保川清

  2017年度生命科学系学会合同年次大会（ConBio2017）, 2017

  
• マウス線維芽細胞株NIH3T3 RNase H2遺伝子欠損株の作製

  月足元希; 兒島憲二; 馬場美聡; 滝田禎亮; 保川清

  2017年度生命科学系学会合同年次大会（ConBio2017）, 2017

  
• MMLV逆転写酵素、耐熱型ポリメラーゼおよびＤＮＡ／ＲＮＡヘリカーゼの組み合わせによる高感度名one-step RT-PCR

  岡野啓志; 馬場美聡; 山崎朋美; 秀瀬涼太; 藤原伸介; 柳原格; 宇治家武史; 林司; 兒島憲二; 滝田禎亮; 保川清

  日本生物工学会29年度大会, 2017

  
• 新規3酵素の組み合わせによる高感度One-step RT-PCRの実現

  岡野啓志; 馬場美聡; 山崎朋美; 秀瀬涼太; 藤原伸介; 柳原格; 宇治家武史; 林司; 兒島憲二; 滝田禎亮; 保川清

  日本農芸化学会関西支部弟499会例会, 2017

  
• ヒトRnaseH2の酵素活性と安定性に与える中性塩とｐHの影響

  馬場美聡; 兒島憲二; 中瀬理保子; 今井翔太; 山崎朋美; Robert; J. Crouch; 滝田禎亮; 保川清

  第64回日本生化学会近畿支部例会, 2017

  
• ビタミンD水酸化酵素CYP105A1とその変異体の精製と性状解析

  佐久間宙; 余語祐哉; 滝田禎亮; 安田佳織; 生城真一; 榊利之; 保川清

  農芸化学会関西支部第498回講演会, 2017

  
• ヒトRNase H2の酵素化学的性質の解析

  馬場美聡; 兒島憲二; 中瀬理保子; 今井翔太; 山崎朋美; Robert; J. Crouch; 滝田禎亮; 保川清

  農芸化学関西支部第496回講演会, 2016

  
• 組換えFlavobacterium psychrophilum コラゲナーゼの発現とアユ冷水病ワクチンへの応用

  森真璃子; 中山仁志; 田中啓友; 服部俊治; 本川祥吾; 桑原正和; 天野健一; 滝田禎亮; 保川清

  農芸化学関西支部第496回講演会, 2016

  
• MMLV逆転写酵素の無細胞タンパク質合成系による発現

  片野 裕太; 久好 哲郎; 久世 郁美; 岡野 啓志; 伊東 昌章; 西垣 功一; 滝田 禎亮; 保川 清

  日本生物高分子学会2016, 2016

  
• ヒトRNase H2の活性と安定性に対する塩の影響

  兒島憲二; 馬場美聡; 中瀬理保子; 今井翔太; 山崎朋美; Robert; J. Crouch; 滝田禎亮; 保川清

  農芸化学会関西支部第495回講演会, 2016

  
• MMLV逆転写酵素の無細胞タンパク質合成系の構築とマイクロアレイへの応用

  片野 裕太; 久好 哲郎; 久世 郁美; 岡野 啓志; 伊東 昌章; 西垣 功一; 滝田 禎亮; 保川 清

  第63回日本生化学会近畿支部例会, 2016

  
• MMLV逆転写酵素の無細胞タンパク質合成系の構築とミクロアレイへの応用

  片野裕太; 久好哲郎; 久世郁美; 岡野啓志; 伊東昌章; 西垣功一; 滝田禎亮; 保川清

  日本農芸化学会2016年度大会, 2016

  
• CYP105A1三重変異体発現Rhodococcus属放線菌を用いたビタミンD水酸化体の生産

  余語祐哉; 安田佳織; 真野寛生; 林恵子; 杉本宏; 滝田禎亮; 保川清; 安武義晃; 田村具博; 太田美穂; 鎌倉昌樹; 生城真一; 榊利之

  日本農芸化学会大会講演要旨集(Web), 05 Mar. 2015

  
• 活性型ビタミンDや医薬品代謝物を生産するCYP105A1の機能解析

  余語祐哉; 安田佳織; 滝田禎亮; 杉本宏; 城宜嗣; 真野寛生; 林恵子; 鎌倉昌樹; 太田美穂; 生城真一; 保川清; 榊利之

  日本農芸化学会中部支部例会講演要旨集(Web), 2015

  
• 放線菌由来CYP105A1の三重変異体(R73A/R84A/M239X)の大腸菌での発現とビタミンD2と7‐メトキシクマリンに対する酵素活性

  佐久間宙; 余語祐哉; 滝田禎亮; 安田佳織; 杉本宏; 城宜嗣; 真野寛生; 太田美穂; 生城真一; 保川清; 榊利之

  日本農芸化学会中部支部例会講演要旨集(Web), 2015

  
• CYP105A1の立体構造に基づく機能改変と医薬品生産への応用

  余語祐哉; 安田佳織; 滝田禎亮; 杉本宏; 城宜嗣; 真野寛生; 林恵子; 鎌倉昌樹; 太田美穂; 生城真一; 保川清; 榊利之

  第38回日本分子生物学会年会第88回日本生化学会大会合同大会, 2015

  
• MMLV逆転写酵素の無細胞タンパク質合成系での発現

  片野裕太; 久好哲郎; 久世郁美; 岡野啓志; 伊東昌章; 西垣功一; 滝田禎亮; 保川清

  第38回日本分子生物学会年会第88回日本生化学会大会合同大会, 2015

  
• 2-IV-27 放線菌由来CYP105A1変異体を用いたビタミンD水酸化体および医薬品代謝物の生産(一般演題要旨,ビタミン・バイオファクター研究のさらなる魅力～大和まほろぱからの発信～,第67回大会講演要旨)

  安田 佳織; 榊 利之; 余語 祐哉; 滝田 禎亮; 保川 清; 杉本 宏; 城 宜嗣; 真野 寛生; 太田 美穂; 生城 真一

  ビタミン, 2015

  
• Protein engineering of actinomycete CYP105A1 to produce the active form of vitamin D2

  Teisuke Takita; Kaori Yasuda; Yuya Yogo; Hiroshi Sugimoto; Yoshitsugu Shiro; Keiko Hayashi; Hiroki Mano; Masaki Kamakura; Miho Ohta; Shinichi Ilkushiro; Kiyoshi Yasukawa; Toshiyuki Sakaki

  第12回シトクロムP450国際シンポジウム, 2014

  
• CYP105A1変異体発現Rhodococcus属放線菌を用いたビタミンD水酸化体の生産

  余語祐哉; 安田佳織; 林恵子; 杉本宏; 滝田禎亮; 保川清; 安武義晃; 田村具博; 太田美穂; 鎌倉昌樹; 生城真一; 榊利之

  日本生化学会大会(Web), 2014

  
• Insights into the reaction mechanism of the coagulation of soy proteins induced by subtilisin Carlsberg

  K. Inouye; M. Nakano; K. Asaoka; K. Yasukawa

  JOURNAL OF BIOTECHNOLOGY, Nov. 2010

  
• Rapid detection of Mycobacterium tuberculosis in sputum samples by using isothermal RNA amplification and real-time fluorescence monitoring.

  K Yasukawa; T Hayashi; S Tsuchiya; Y Isawa; H Suzuki; S Takakura; S Ichiyama; K Suzuki

  CLINICAL CHEMISTRY, Jun. 2004

  
• Rapid detection of M-avium complex and M-kansasii in sputum samples by using isothermal RNA amplification and real-time fluorescence monitoring.

  T Hayashi; K Yasukawa; S Tsuchiya; Y Isawa; H Suzuki

  CLINICAL CHEMISTRY, Jun. 2004

  
• Ultrarapid detection of gene expression in MRSA and M-tuberculosis by real-time fluorescence monitoring of isothermal RNA amplification with INAF DNA probe.

  K Yasukawa; T Ishiguro

  CLINICAL CHEMISTRY, Nov. 2001

  
• Expression of interleukin-6 receptor on human cord blood- and peripheral blood-derived primitive hematopoietic progenitors implies an acquisition of different functional properties.

  Y Sonoda; H Minamiguchi; N Yahata; T Kimura; H Fujiki; S Harada; J Wang; K Okuda; H Kaneko; K Yasukawa; JH Ohyashiki; K Ohyashiki; T Abe

  BLOOD, Nov. 1999

  
• The combination of soluble IL-6 receptor and IL-6 induces tyrosine phosphorylation of GP130, Jak1, Jak2 and STAT3 in human platelets.

  A Oda; T Nakahata; BJ Druker; K Ozaki; K Tsuji; K Yasukawa; T Kato; M Handa; Y Ikeda

  BLOOD, Nov. 1997

  
• Discrimination of liver cirrhosis from chronic hepatitis by the determination of the ratio of Aleuria aurantia lectin-reactive serum cholinesterase to immunoreactive one.

  T Hada; M Kondo; K Yasukawa; A Iwasaki; H Iijima; Y Amuro; K Higashino

  HEPATOLOGY, Oct. 1997

  
• Lineage-specific factor independent blood cell production: Analysis of gp130 signaling in hematopoiesis.

  T Kimura; H Sakabe; S Tanimukai; Y Ohmizono; T Abe; K Yasukawa; T Taga; Y Sonoda

  BLOOD, Nov. 1996

  
• Interactions between JAK-STAT and MAP kinase pathways in bone cells in response to IL-6 superfamily cytokines.

  R Nishimura; Y Kato; K Yasukawa; T Michigami; GR Mundy; T Yoneda

  JOURNAL OF BONE AND MINERAL RESEARCH, Aug. 1996

  
• The effects of anti-human gp130 antibody on bone in normal and ovariectomized rats.

  S Nishida; T Nakamura; Y Tanaka; N Okimoto; Y Okazaki; M Kumegawa; K Yasukawa; M Murayama; K Suzuki

  JOURNAL OF BONE AND MINERAL RESEARCH, Aug. 1996

  
• Structure-function relationship of human interleukin-6: Site-directed mutagenesis and NMR studies.

  西村千秋; 保川清; 嶋田一夫; 岸本忠三; 荒田洋治

  タンパク質構造討論会講演要旨集, Aug. 1996

  
• Ex vivo expansion of human primitive hemopoietic progenitor cells using recombinant soluble IL-6 receptor (sIL-6R)

  T Nakahata; Sui, X; S Tajima; K Tsuji; K Yasukawa; T Taga; T Kishimoto

  BLOOD, Nov. 1995

  
• IL-6/SOLUBLE IL-6-RECEPTOR (SIL-6R) MEDIATE 2 DISTINCT SIGNALING PATHWAYS IN OSTEOBLASTS THROUGH GP130

  R NISHIMURA; K YASUKAWA; GR MUNDY; T YONEDA

  JOURNAL OF BONE AND MINERAL RESEARCH, Aug. 1995

  
• GP130 AND C-KIT SIGNALINGS SYNERGIZE FOR EX-VIVO EXPANSION OF HUMAN PRIMITIVE HEMATOPOIETIC PROGENITOR CELLS

  XW SUI; K TSUJI; R TANAKA; S TAJIMA; K MURAOKA; Y EBIHARA; K IKEBUCHI; K YASUKAWA; T TAGA; T KISHIMOTO; T NAGAHATA

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Mar. 1995, Peer-reviewed

  
• ACTIVATION OF JAK2 KINASE MEDIATED BY THE INTERLEUKIN-6 SIGNAL TRANSDUCER GP130

  M NARAZAKI; BA WITTHUHN; K YOSHIDA; O SILVENNOINEN; K YASUKAWA; JN IHLE; T KISHIMOTO; T TAGA

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Mar. 1994, Peer-reviewed

  
• IL-6-INDUCED HOMODIMERIZATION OF GP130 AND ASSOCIATED ACTIVATION OF A TYROSINE KINASE

  M MURAKAMI; M HIBI; N NAKAGAWA; T NAKAGAWA; K YASUKAWA; K YAMANISHI; T TAGA; T KISHIMOTO

  JOURNAL OF CELLULAR BIOCHEMISTRY, 1994

  
• FUNCTIONAL INHIBITION OF HEMATOPOIETIC AND NEUROTROPHIC CYTOKINES BY BLOCKING THE INTERLEUKIN-6 SIGNAL TRANSDUCER GP130

  T TAGA; M NARAZAKI; K YASUKAWA; T SAITO; D MIKI; M HAMAGUCHI; S DAVIS; M SHOYAB; GD YANCOPOULOS; T KISHIMOTO

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Nov. 1992, Peer-reviewed

  
• CRITICAL CYTOPLASMIC REGION OF THE INTERLEUKIN-6 SIGNAL TRANSDUCER GP130 IS CONSERVED IN THE CYTOKINE RECEPTOR FAMILY

  M MURAKAMI; M NARAZAKI; M HIBI; H YAWATA; K YASUKAWA; M HAMAGUCHI; T TAGA; T KISHIMOTO

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Dec. 1991, Peer-reviewed

  
• IL‐6R binding peptide and its structur.

  駅田悌二; 舛田晋; 西村千秋; 保川清; 二木研輔; 岸本忠三; 荒田洋治

  日本免疫学会総会・学術集会記録, Oct. 1991

  
• Chemical modifications and IL‐6 activity.

  二木研輔; 西村千秋; 駅田悌二; 保川清; 岸本忠三; 荒田洋治

  日本免疫学会総会・学術集会記録, Oct. 1990

  
• HUMAN B-CELL DIFFERENTIATION FACTOR DEFINED BY AN ANTIPEPTIDE ANTIBODY AND ITS POSSIBLE ROLE IN AUTOANTIBODY PRODUCTION

  T HIRANO; T TAGA; K YASUKAWA; K NAKAJIMA; N NAKANO; F TAKATSUKI; M SHIMIZU; A MURASHIMA; S TSUNASAWA; F SAKIYAMA; T KISHIMOTO

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Jan. 1987, Peer-reviewed

  
• PURIFICATION TO HOMOGENEITY AND CHARACTERIZATION OF HUMAN B-CELL DIFFERENTIATION FACTOR (BCDF OR BSFP-2)

  T HIRANO; T TAGA; N NAKANO; K YASUKAWA; S KASHIWAMURA; K SHIMIZU; K NAKAJIMA; KH PYUN; T KISHIMOTO

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, Peer-reviewed

  

Presentations

• Characterization of Moloney murine leukemia virus/avian myeloblastosis virus chimeric reverse transcriptase

  RNase H 2012, 05 Sep. 2012

  
• タンパク質工学による逆転写酵素の耐熱化（シンポジウム：タンパク質工学による耐熱化酵素創製の新しい戦略）

  2011年度日本農芸化学会大会, 28 Mar. 2011

  
• 逆転写酵素の活性と熱安定性（シンポジウム：核酸に関わる酵素の化学）

  2009年度日本農芸化学会大会, 29 Mar. 2009

  
• 基質特異性を改変させたことにより自己活性化能を消失したプロテアーゼの大腸菌での生産（タンパク質発現手法の最適化－より良い機能性タンパク質を求めて）

  2009年度日本農芸化学会大会, 28 Mar. 2009

  

Books and Other Publications

• Essential Protein Engineering

  Oikawa, N; Oshima, T; Yasukawa, K; Mihara, H; Miyahara, I, Joint work

  Kodansha, 2018, Not refereed

  
• アスパルテームの合成

  井上國世; 保川清

  酵素利用技術大系（小宮山眞監修）p. 632-636、株式会社エヌ・ティー・エス、東京、2010, 2010, Not refereed

  
• サーモライシンの応用と高機能化

  井上國世; 橋田泰彦; 草野正雪; 保川清

  産業酵素の応用技術と最新動向（井上國世、監修）p. 58-68、シーエムシー出版、東京、2009, 2009, Not refereed

  
• 核酸増幅法

  保川清

  産業酵素の応用技術と最新動向（井上國世、監修）p. 194-202、シーエムシー出版、東京、2009, 2009, Not refereed

  
• Development of cDNA synthesis technology by a combination of novel enzymes

  Okano, H; Yasukawa, K, Joint work, 日本生物工学会誌

  日本生物工学会, Not refereed

  

Awards

• 1996

  日本生物工学会論文賞Excellent Paper Aword of the Society for Biotechnology, Japan

External funds: Kakenhi

• Rapid detection of mutation in HIV-1 reverse transcriptase gene by temperature gradient gel electrophoresis

  Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

  Medium-sized Section 62:Applied informatics and related fields

  Kyoto University

  Kiyoshi YASUKAWA

  From 07 Feb. 2019, To 31 Mar. 2023, Project Closed

  ゲノムプロフィリング;HIV-1;エイズ;SARS-CoV-19;変異;温度勾配ゲル電気泳動;画像処理;データベース;逆転写酵素;耐性変異;RNA増幅法

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18KK0285/
• Development and application of genome profiling system featuring high-speed and large image processing

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 62:Applied informatics and related fields

  Kyoto University

  Kiyoshi Yasukawa

  From 29 Jun. 2018, To 31 Mar. 2021, Project Closed

  ゲノムプロフィリング;PCR;リコンビナーゼポリメラーゼ増幅法;RPA;温度勾配ゲル電気泳動;画像処理;変曲点;データベース;ゲノム;マイクロアレイ

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K19839/
• Elucidation of the mecahsim of thermostabilization of thermostable reverse transcriptase

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  KIYOSHI YASUKAWA

  From 01 Apr. 2015, To 31 Mar. 2018, Project Closed

  リボヌクレアーゼH;RNase H;RNase H2;逆転写酵素;GPMA method;CRISPR/Cas9システム;変異原性;MMLV RT;ゲノムプロフィリング法;耐熱化機構

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K07388/
• Evaluation of cDNA synthesis and RNA amplification with highly efficient reverse transcriptases and expansion of their use

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Kiyoshi YASUKAWA

  From 01 Apr. 2009, To 31 Mar. 2012, Project Closed

  酵素利用学;ウイルス;核酸;酵素;酵素反応;バイオテクノロジー

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580110/
• Protein engineering and reaction control technology targeting thermolysin for the expansion of its use in food industry

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Kuniyo INOUYE

  From 01 Apr. 2008, To 31 Mar. 2011, Project Closed

  酵素化学酵素利用学;酵素;蛋白質工学;反応制御;ペプチド;食品;プロテアーゼ;サーモライシン;部位特異的変異導入

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380061/
• Expression of recombinant AMV reverse transcriptase and improvement of its productivity, activity, and stability by protein engineering

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Kiyoshi YASUKAWA

  From 01 Apr. 2007, To 31 Mar. 2009, Project Closed

  酵素利用学;ウイルス;核酸;酵素;酵素反応;バイオテクノロジー

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19580104/
• Protein engineering of thermolysin and reaction-control engineering for the aspartame synthesis using thermolysin

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Kuniyo INOUYE

  From 01 Apr. 2005, To 31 Mar. 2008, Project Closed

  酵素;蛋白質工学;反応制御;ペプチド;食品;プロテアーゼ;サーモライシン;部位特異的変異導入, enzyme;Protein engineering;reaction control;Peptide;food;protease;thermolysin;site-directed mutagenesis

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380065/
• 高機能等温核酸増幅酵素の創製と構造解析および病原体の現場での迅速検出への応用

  Grant-in-Aid for Scientific Research (B)

  Basic Section 58020:Hygiene and public health-related: including laboratory approach

  Kyoto University

  保川 清

  From 01 Apr. 2022, To 31 Mar. 2025, Granted

  リコンビナーゼポリメラーゼ増幅法;リコンビナーゼ;一本鎖DNA結合タンパク質;鎖置換DNAポリメラーゼ;ATP再生酵素;等温核酸増幅法;ピルビン酸キナーゼ;uvsY;鎖置換DNAポリメ ラーゼ

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H03332/
• 高機能等温核酸増幅酵素の創製と構造解析および病原体の現場での迅速検出への応用

  Grant-in-Aid for Scientific Research (B)

  Basic Section 58020:Hygiene and public health-related: including laboratory approach

  Kyoto University

  保川 清

  From 01 Apr. 2022, To 31 Mar. 2025, Granted

  リコンビナーゼポリメラーゼ増幅法;リコンビナーゼ;一本鎖DNA結合タンパク質;鎖置換DNAポリメラーゼ;ATP再生酵素;等温核酸増幅法;ピルビン酸キナーゼ;uvsY;鎖置換DNAポリメ ラーゼ

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K24590/

Education

Teaching subject(s)

• From 01 Apr. 2024, To 31 Mar. 2025

  Advanced Course of Protein Engineering

  GA23, Spring, Graduate School of Agriculture, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Introduction to Food Bioscience

  7329, Spring, Faculty of Agriculture, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar on Biochemistry and Microbiology

  7317, Fall, Faculty of Agriculture, 1
• From 01 Apr. 2024, To 31 Mar. 2025

  Enzyme Chemistry Seminar

  GF01, Spring, Graduate School of Agriculture, 1.5
• From 01 Apr. 2024, To 31 Mar. 2025

  Enzyme Chemistry Seminar

  GF01, Fall, Graduate School of Agriculture, 1.5
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course of Enzyme Chemistry

  GG01, Fall, Graduate School of Agriculture, 3
• From 01 Apr. 2024, To 31 Mar. 2025

  Experimental Course of Enzyme Chemistry

  GG01, Spring, Graduate School of Agriculture, 3
• From 01 Apr. 2024, To 31 Mar. 2025

  Laboratory Course in Food Biochemistry

  7325, Spring, Faculty of Agriculture, 3
• From 01 Apr. 2024, To 31 Mar. 2025

  Enzyme Chemistry

  7227, Fall, Faculty of Agriculture, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Applied Enzyme Chemistry

  7152, Fall, Faculty of Agriculture, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Advanced Course of Enzyme Chemistry

  GA22, Spring, Graduate School of Agriculture, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Biochemistry and Microbiology

  7317, Fall, Faculty of Agriculture, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Thesis

  7273, Year-long, Faculty of Agriculture, 10
• From 01 Apr. 2023, To 31 Mar. 2024

  Laboratory Course in Food Biochemistry

  7325, Spring, Faculty of Agriculture, 3
• From 01 Apr. 2023, To 31 Mar. 2024

  Enzyme Chemistry Seminar1

  GB09, Year-long, Graduate School of Agriculture, 3
• From 01 Apr. 2023, To 31 Mar. 2024

  Enzyme Chemistry Seminar2

  GB10, Year-long, Graduate School of Agriculture, 3
• From 01 Apr. 2023, To 31 Mar. 2024

  Experimental Course of Enzyme Chemistry2

  GC10, Year-long, Graduate School of Agriculture, 6
• From 01 Apr. 2023, To 31 Mar. 2024

  Experimental Course of Enzyme Chemistry1

  GC09, Year-long, Graduate School of Agriculture, 6
• From 01 Apr. 2023, To 31 Mar. 2024

  Enzyme Chemistry

  7227, Fall, Faculty of Agriculture, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Introduction to Food Bioscience

  7215, Spring, Faculty of Agriculture, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Applied Enzyme Chemistry

  7152, Fall, Faculty of Agriculture, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Food Molecular Biology I

  7309, Fall, Faculty of Agriculture, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Enzyme Chemistry Seminar2

  GB10, Year-long, Graduate School of Agriculture, 3
• From 01 Apr. 2022, To 31 Mar. 2023

  Introduction to Food Bioscience

  7215, Spring, Faculty of Agriculture, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Thesis

  7273, Year-long, Faculty of Agriculture, 10
• From 01 Apr. 2022, To 31 Mar. 2023

  Applied Enzyme Chemistry

  7152, Fall, Faculty of Agriculture, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course of Enzyme Chemistry1

  GC09, Year-long, Graduate School of Agriculture, 6
• From 01 Apr. 2022, To 31 Mar. 2023

  Enzyme Chemistry Seminar1

  GB09, Year-long, Graduate School of Agriculture, 3
• From 01 Apr. 2022, To 31 Mar. 2023

  Enzyme Chemistry

  7227, Fall, Faculty of Agriculture, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Biochemistry and Microbiology

  7317, Fall, Faculty of Agriculture, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Laboratory Course in Food Biochemistry

  7325, Spring, Faculty of Agriculture, 3
• From 01 Apr. 2022, To 31 Mar. 2023

  Experimental Course of Enzyme Chemistry2

  GC10, Year-long, Graduate School of Agriculture, 6
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Course of Protein Engineering

  GA23, Spring, Graduate School of Agriculture, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Food Science and Production Technology

  7277, Spring, Faculty of Agriculture, 2
• Introduction to the Molecular Cell Biology
• Moelcular Biology of Foods
• Enzyme Chemistry
• Laboratory Course in Enzyme Chemistry and Biochemistry
• From Apr. 2011, To Mar. 2012

  酵素の作用と応用

  Fall, 農学部
• From Apr. 2011, To Mar. 2012

  食品生化学I

  Spring, 農学部
• From Apr. 2011, To Mar. 2012

  食品生化学Ⅱ

  Fall, 農学部
• From Apr. 2011, To Mar. 2012

  酵素化学

  Fall, 農学部
• From Apr. 2011, To Mar. 2012

  酵素化学・生化学実験及び実験法

  Spring, 農学部
• From Apr. 2011, To Mar. 2012

  酵素化学専攻実験

  農学研究科
• From Apr. 2011, To Mar. 2012

  酵素化学専攻演習

  農学研究科
• From Apr. 2011, To Mar. 2012

  酵素化学特論

  農学研究科
• From Apr. 2012, To Mar. 2013

  Enzymes: Function and Application

  Fall, 農学部
• From Apr. 2012, To Mar. 2013

  Enzyme Chemistry

  Fall, 農学部
• From Apr. 2012, To Mar. 2013

  Laboratory Course in Enzyme Chemistry

  Spring, 農学部
• From Apr. 2012, To Mar. 2013

  Uses of enzymes in sensitive analyses

  Spring, 全学共通科目
• From Apr. 2012, To Mar. 2013

  Food Biochemistry I

  Spring, 農学部
• From Apr. 2012, To Mar. 2013

  Food Biochemistry II

  Fall, 農学部
• From Apr. 2013, To Mar. 2014

  Enzymes: Function and Application

  Fall, 農学部
• From Apr. 2013, To Mar. 2014

  Food Biochemistry I

  Spring, 農学部
• From Apr. 2013, To Mar. 2014

  Food Biochemistry II

  Fall, 農学部
• From Apr. 2013, To Mar. 2014

  Enzyme Chemistry

  Fall, 農学部
• From Apr. 2013, To Mar. 2014

  Laboratory Course in Enzyme Chemistry

  Spring, 農学部
• From Apr. 2013, To Mar. 2014

  Enzyme Chemistry (Advanced Course)

  Fall, 農学研究科
• From Apr. 2013, To Mar. 2014

  Enzyme Chemistry Seminar

  Year-long, 農学研究科
• From Apr. 2013, To Mar. 2014

  Experimental Course of Enzyme Chemistry

  Year-long, 農学研究科
• From Apr. 2013, To Mar. 2014

  Uses of enzymes in sensitive analyses

  Spring, 全学共通科目
• From Apr. 2014, To Mar. 2015

  Enzymes: Function and Application

  Fall, 農学部
• From Apr. 2014, To Mar. 2015

  Food Biochemistry I

  Spring, 農学部
• From Apr. 2014, To Mar. 2015

  Food Biochemistry II

  Fall, 農学部
• From Apr. 2014, To Mar. 2015

  Enzyme Chemistry

  Fall, 農学部
• From Apr. 2014, To Mar. 2015

  Laboratory Course in Enzyme Chemistry

  Spring, 農学部
• From Apr. 2014, To Mar. 2015

  Analytical Chemistry in Food Science

  Fall, 農学部
• From Apr. 2014, To Mar. 2015

  Enzyme Chemistry Seminar

  Year-long, 農学研究科
• From Apr. 2014, To Mar. 2015

  Experimental Course of Enzyme Chemistry

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Enzymes: Function and Application

  Fall, 農学部
• From Apr. 2015, To Mar. 2016

  Enzyme Chemistry

  Fall, 農学部
• From Apr. 2015, To Mar. 2016

  Laboratory Course in Enzyme Chemistry

  Spring, 農学部
• From Apr. 2015, To Mar. 2016

  Experimental Course of Enzyme Chemistry1

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Experimental Course of Enzyme Chemistry

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Enzyme Chemistry Seminar1

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Enzyme Chemistry Seminar

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Enzyme Chemistry (Advanced Course)

  Fall, 農学研究科
• From Apr. 2015, To Mar. 2016

  Analytical Chemistry in Food Science

  Fall, 農学部
• From Apr. 2015, To Mar. 2016

  Food Biochemistry I

  Spring, 農学部
• From Apr. 2015, To Mar. 2016

  Food Biochemistry II

  Fall, 農学部
• From Apr. 2016, To Mar. 2017

  Enzyme Chemistry

  Fall, 農学部
• From Apr. 2016, To Mar. 2017

  Laboratory Course in Enzyme Chemistry

  Spring, 農学部
• From Apr. 2016, To Mar. 2017

  Experimental Course of Enzyme Chemistry1

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Experimental Course of Enzyme Chemistry2

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Experimental Course of Enzyme Chemistry

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Enzyme Chemistry Seminar1

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Enzyme Chemistry Seminar2

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Enzyme Chemistry Seminar

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Analytical Chemistry in Food Science

  Fall, 農学部
• From Apr. 2016, To Mar. 2017

  Principles of Molecular Biology in Food Science II

  Spring, 農学部
• From Apr. 2016, To Mar. 2017

  Introduction to Food Bioscience

  Spring, 農学部
• From Apr. 2017, To Mar. 2018

  Fundamental Biochemistry II

  Fall, 農学部
• From Apr. 2017, To Mar. 2018

  Applied Enzyme Chemistry

  Fall, 農学部
• From Apr. 2017, To Mar. 2018

  Enzyme Chemistry

  Fall, 農学部
• From Apr. 2017, To Mar. 2018

  Experimental Course of Enzyme Chemistry1

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Experimental Course of Enzyme Chemistry2

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Enzyme Chemistry Seminar1

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Enzyme Chemistry Seminar2

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Advanced Course of Enzyme Chemistry

  Spring, 農学研究科
• From Apr. 2017, To Mar. 2018

  Analytical Chemistry in Food Science

  Fall, 農学部
• From Apr. 2017, To Mar. 2018

  Principles of Molecular Biology in Food Science II

  Spring, 農学部
• From Apr. 2017, To Mar. 2018

  Laboratory Course in Food Biochemistry (I)

  Spring, 農学部
• From Apr. 2018, To Mar. 2019

  Fundamental Biochemistry II

  Fall, 農学部
• From Apr. 2018, To Mar. 2019

  Applied Enzyme Chemistry

  Fall, 農学部
• From Apr. 2018, To Mar. 2019

  Advanced Course of Protein Engineering

  Spring, 農学研究科
• From Apr. 2018, To Mar. 2019

  Enzyme Chemistry

  Fall, 農学部
• From Apr. 2018, To Mar. 2019

  Experimental Course of Enzyme Chemistry1

  Year-long, 農学研究科
• From Apr. 2018, To Mar. 2019

  Experimental Course of Enzyme Chemistry2

  Year-long, 農学研究科
• From Apr. 2018, To Mar. 2019

  Enzyme Chemistry Seminar1

  Year-long, 農学研究科
• From Apr. 2018, To Mar. 2019

  Enzyme Chemistry Seminar2

  Year-long, 農学研究科
• From Apr. 2018, To Mar. 2019

  Analytical Chemistry in Food Science

  Fall, 農学部
• From Apr. 2018, To Mar. 2019

  Principles of Molecular Biology in Food Science II

  Spring, 農学部
• From Apr. 2018, To Mar. 2019

  Laboratory Course in Food Biochemistry (I)

  Spring, 農学部
• From Apr. 2018, To Mar. 2019

  Seminar on Food Science and Biotechnology

  Fall, 農学部
• From Apr. 2019, To Mar. 2020

  Applied Enzyme Chemistry

  Fall, 農学部
• From Apr. 2019, To Mar. 2020

  Seminar on Biochemistry and Microbiology

  Fall, 農学部
• From Apr. 2019, To Mar. 2020

  Enzyme Chemistry

  Fall, 農学部
• From Apr. 2019, To Mar. 2020

  Experimental Course of Enzyme Chemistry1

  Year-long, 農学研究科
• From Apr. 2019, To Mar. 2020

  Experimental Course of Enzyme Chemistry2

  Year-long, 農学研究科
• From Apr. 2019, To Mar. 2020

  Enzyme Chemistry Seminar1

  Year-long, 農学研究科
• From Apr. 2019, To Mar. 2020

  Enzyme Chemistry Seminar2

  Year-long, 農学研究科
• From Apr. 2019, To Mar. 2020

  Advanced Course of Enzyme Chemistry

  Spring, 農学研究科
• From Apr. 2019, To Mar. 2020

  Physical Chemistry in Food Science I

  Fall, 農学部
• From Apr. 2019, To Mar. 2020

  Laboratory Course in Food Biochemistry

  Spring, 農学部
• From Apr. 2020, To Mar. 2021

  Applied Enzyme Chemistry

  Fall, 農学部
• From Apr. 2020, To Mar. 2021

  Seminar on Biochemistry and Microbiology

  Spring, 農学部
• From Apr. 2020, To Mar. 2021

  Advanced Course of Protein Engineering

  Spring, 農学研究科
• From Apr. 2020, To Mar. 2021

  Enzyme Chemistry

  Fall, 農学部
• From Apr. 2020, To Mar. 2021

  Experimental Course of Enzyme Chemistry1

  Year-long, 農学研究科
• From Apr. 2020, To Mar. 2021

  Experimental Course of Enzyme Chemistry2

  Year-long, 農学研究科
• From Apr. 2020, To Mar. 2021

  Enzyme Chemistry Seminar1

  Year-long, 農学研究科
• From Apr. 2020, To Mar. 2021

  Enzyme Chemistry Seminar2

  Year-long, 農学研究科
• From Apr. 2020, To Mar. 2021

  Physical Chemistry in Food Science I

  Fall, 農学部
• From Apr. 2020, To Mar. 2021

  Laboratory Course in Food Biochemistry

  Spring, 農学部
• From Apr. 2021, To Mar. 2022

  Applied Enzyme Chemistry

  Fall, 農学部
• From Apr. 2021, To Mar. 2022

  Seminar on Biochemistry and Microbiology

  Fall, 農学部
• From Apr. 2021, To Mar. 2022

  Enzyme Chemistry

  Fall, 農学部
• From Apr. 2021, To Mar. 2022

  Experimental Course of Enzyme Chemistry1

  Year-long, 農学研究科
• From Apr. 2021, To Mar. 2022

  Experimental Course of Enzyme Chemistry2

  Year-long, 農学研究科
• From Apr. 2021, To Mar. 2022

  Enzyme Chemistry Seminar1

  Year-long, 農学研究科
• From Apr. 2021, To Mar. 2022

  Enzyme Chemistry Seminar2

  Year-long, 農学研究科
• From Apr. 2021, To Mar. 2022

  Advanced Course of Enzyme Chemistry

  Spring, 農学研究科
• From Apr. 2021, To Mar. 2022

  Physical Chemistry in Food Science I

  Fall, 農学部
• From Apr. 2021, To Mar. 2022

  Laboratory Course in Food Biochemistry

  Spring, 農学部
• From Apr. 2021, To Mar. 2022

  Introduction to Food Bioscience

  Spring, 農学部

Participation in PhD Defense

• Biological Evaluation of the Aplysiatoxin Analogue in HIV Cure Strategy and Development of New PKC Ligands with Isozyme Selectivity Using a Machine Learning

  MAKI JUMPEI, Graduate School of Agriculture, Sub-chief Examiner

  24 Mar. 2025
• Synthesis and structure-function analyses of propeller- and parallel-type trimer models of amyloid β40

  UCHINO AYUMI, Graduate School of Agriculture, Sub-chief Examiner

  24 Mar. 2025
• Studies on the Biological Functionality of Cellulose Nanofiberand Its Derivative as Possible Food Additives

  NUNTANUT POPUANG, Graduate School of Agriculture, Sub-chief Examiner

  24 Sep. 2024
• Structure-Function Analysis of Conformationally Fixed Analogs of Amyloid β42 and Development of Antibodies against the Toxic Conformer

  MATSUSHIMA YUKA, Graduate School of Agriculture, Sub-chief Examiner

  24 Mar. 2025
• Studies on enzymes and reaction conditions in recombinase polymerase amplification

  KEVIN MAAFU JUMA, Graduate School of Agriculture, Chief Examiner

  25 Mar. 2024
• Studies on inhibition of α-amylase and α-glucosidase by components of Morus australis

  Ying Qiao, Graduate School of Agriculture, Chief Examiner

  26 Sep. 2022
• Studies on the pathological mechanism of alopecia areata in C3H/HeJ mouse model

  HASHIMOTO KEI, Graduate School of Agriculture, Sub-chief Examiner

  25 Jul. 2022
• Studies on the activities of serine proteases from Ficus carica

  NISHIMURA KOSAKU, Graduate School of Agriculture, Chief Examiner

  26 Jul. 2021
• Studies on the structural modification of protein aggregate induced by freezing process

  Fang Bowen, Graduate School of Agriculture, Sub-chief Examiner

  24 Sep. 2021
• Studies on the anemia-improving effect of prolyl hydroxylase inhibitors

  KATO SOTA, Graduate School of Agriculture, Sub-chief Examiner

  23 Mar. 2022

Administration

School management (title, position)

• From 01 Apr. 2013, To 31 Mar. 2015

  放射線障害予防小委員会 委員
• From 01 Apr. 2017, To 31 Mar. 2019

  学生生活委員会 委員
• From 01 Apr. 2017, To 31 Mar. 2019

  学生生活委員会第一小委員会 委員
• From 01 Apr. 2017, To 31 Mar. 2019

  京都大学奨学金返還免除候補者選考委員会 委員
• From 01 Apr. 2017, To 31 Mar. 2019

  学生生活委員会第一小委員会 委員長
• From 01 Apr. 2017, To 31 Mar. 2019

  京都大学奨学金返還免除候補者選考委員会 委員長
• From 31 Aug. 2017, To 31 Mar. 2018

  ＳＰＥＣ（京大生チャレンジコンテスト）2017選考委員会
• From 01 Apr. 2019, To 31 Mar. 2020

  農芸化学系長
• From 01 Jan. 2019, To 31 Dec. 2019

  国際教育委員会国際学生交流委員会アムジェン・スカラーズ・プログラム小委員会 委員
• From 01 Jan. 2020, To 31 Dec. 2020

  国際教育委員会国際学生交流委員会アムジェン・スカラーズ・プログラム小委員会 委員
• From 01 Jan. 2019, To 31 Dec. 2021

  国際教育委員会国際学生交流委員会アムジェン・スカラーズ・プログラム小委員会 委員

Faculty management (title, position)

• From 01 Apr. 2023, To 31 Mar. 2024

  食品生物科学専攻　専攻長
• From 01 Apr. 2023, To 31 Mar. 2024

  防火・防災委員会　委員
• From 01 Apr. 2022, To 31 Mar. 2024

  評価委員会　委員
• From 01 Apr. 2022, To 31 Mar. 2023

  食品生物科学科長
• From 01 Apr. 2013, To 31 Mar. 2015

  学生生活委員会　委員
• From 01 Apr. 2015, To 31 Mar. 2017

  制度・組織等検討検討委員会　委員
• From 01 Apr. 2015, To 31 Mar. 2017

  男女共同参画推進委員会　委員
• From 01 Apr. 2015, To 31 Mar. 2016

  食品生物科学科長
• From 01 Apr. 2015, To 31 Mar. 2018

  評価委員会　委員
• From 01 Apr. 2013, To 31 Mar. 2017

  放射線障害防止委員会　委員
• From 01 Apr. 2016, To 31 Mar. 2018

  食品生物科学専攻　専攻長
• From 01 Apr. 2016, To 31 Mar. 2018

  防火・防災委員会　委員
• From 01 Apr. 2017, To 31 Mar. 2019

  研究科教務委員会　委員
• From 01 Apr. 2017, To 31 Mar. 2019

  学部教務委員会　委員
• From 01 Apr. 2017, To 31 Mar. 2019

  人権問題対策委員会
• From 01 Apr. 2018, To 31 Mar. 2020

  比較農業論講座運営協議員会　協議員
• From 21 Nov. 2019, To 31 Mar. 2023

  研究活動推進委員会　委員