Basic Information

Faculty

• 農学部

Professional Memberships

• To Present

  リグニン学会
• To Present

  日本木材学会
• To Present

  糸状菌分子生物学研究会
• To Present

  糸状菌遺伝子研究会

Academic Degree

• 修士（理学）(東京工業大学)
• 博士（理学）(東京工業大学)

Academic Resume (Graduate Schools)

• 東京工業大学, 大学院生命理工学研究科分子生命科学専攻博士前期課程, 修了
• 東京工業大学, 大学院生命理工学研究科分子生命科学専攻博士後期課程, 修了

Academic Resume (Undergraduate School/Majors)

• 東京工業大学, 生命理工学部生命科学科, 卒業

High School

• High School

  大阪府立豊中高校

Research History

• From Jul. 2013, To Present

  Kyoto University, Graduate School of Agriculture, 助教
• From Apr. 2012, To Jun. 2013

  University of Shizuoka, 大学院薬学研究科, 客員共同研究員（発酵研究所若手研究助成）
• From Apr. 2011, To Mar. 2012

  University of Shizuoka, 大学院薬学研究科, 特任助教
• From Apr. 2009, To Mar. 2011

  日本学術振興会 特別研究員（PD）岡山大学
• From Apr. 2008, To Mar. 2009

  日本学術振興会 特別研究員（DC1）東京工業大学

ID,URL

• ORCID ID

  0000-0002-9654-2124

• J-Global ID

  201401058492672021

• Researcher number

  80608141

Website(s) (URL(s))

• http://www.biomass.kais.kyoto-u.ac.jp/

researchmap URL

• https://researchmap.jp/7000009450

Research

Research Topics, Overview of the research

• Research Topics

  
  ・　白色腐朽菌による木質バイオマス生分解システム
  ・　木材腐朽と子実体（キノコ）発生の関連性
  ・　天然物（二次代謝産物）生合成機構と生合成成遺伝子

• Overview of the research

  主な研究成果：
  ・ 順遺伝学による、キノコ発生開始および白色腐朽に必須な遺伝子の多数特定
  ・　世界初のキノコ（ヒトヨタケ）における高頻度遺伝子ターゲティング法の実現
  ・　キノコ（ヒトヨタケ・ヒラタケ）における多重遺伝子改変法の確立
  ・　特定二次代謝産物を介した菌類の形態形成制御・転写調節機構の発見

Research Areas

• Life sciences, Wood science

Papers

• Role of putative APSES family transcription factor Swi6 in cell wall synthesis regulation in the agaricomycete Pleurotus ostreatus

  Hayase Kojima; Takaya Izumi; Moriyuki Kawauchi; Yuitsu Otsuka; Kenya Tsuji; Akira Yoshimi; Chihiro Tanaka; Shigekazu Yano; Takehito Nakazawa; Yoichi Honda

  Fungal Biology, Dec. 2024, Peer-reviewed

  
• Physiological function of hydrophobin Hydph16 in cell wall formation in agaricomycete Pleurotus ostreatus

  Junxian Han; Moriyuki Kawauchi; Yuki Terauchi; Kenya Tsuji; Akira Yoshimi; Chihiro Tanaka; Takehito Nakazawa; Yoichi Honda

  Fungal Genetics and Biology, Nov. 2024, Peer-reviewed

  
• Disruption of the pkac2 gene in Pleurotus ostreatus alters cell wall structures and enables mycelial dispersion in liquid culture.

  Yuitsu Otsuka; Moriyuki Kawauchi; Kai Yoshizawa; Saori Endo; Kim Schiphof; Kenya Tsuji; Akira Yoshimi; Chihiro Tanaka; Shigekazu Yano; Vladimir Elisashvili; Takehito Nakazawa; Toshikazu Irie; Yoichi Honda

  FEMS microbiology letters, 20 Nov. 2024, Peer-reviewed

  
• Effects of hap2 deletion on mnp/vp transcription in Pleurotus ostreatus grown on lignocellulosic substrates.

  Keita Kayama; Takehito Nakazawa; Iori Yamaguchi; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Applied microbiology and biotechnology, 13 Nov. 2024, Peer-reviewed, Corresponding author

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• Putative APSES family transcription factor mbp1 plays an essential role in regulating cell wall synthesis in the agaricomycete Pleurotus ostreatus.

  Hayase Kojima; Moriyuki Kawauchi; Yuitsu Otsuka; Kim Schiphof; Kenya Tsuji; Akira Yoshimi; Chihiro Tanaka; Shigekazu Yano; Takehito Nakazawa; Yoichi Honda

  Fungal genetics and biology : FG & B, 04 Oct. 2024, Peer-reviewed

  
• Functional analysis of basidiomycete specific chitin synthase genes in the agaricomycete fungus Pleurotus ostreatus

  Kim Schiphof; Moriyuki Kawauchi; Kenya Tsuji; Akira Yoshimi; Chihiro Tanaka; Takehito Nakazawa; Yoichi Honda

  Fungal Genetics and Biology, Jun. 2024, Peer-reviewed

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• Identification of two genes essential for basidiospore formation during the postmeiotic stages in Pleurotus ostreatus.

  Takeshi Kobukata; Takehito Nakazawa; Fuga Yamasaki; Junko Sugano; Minji Oh; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Fungal genetics and biology : FG & B, 17 Mar. 2024, Peer-reviewed

  
• Pleurotus ostreatus as a model mushroom in genetics, cell biology, and material sciences.

  Takehito Nakazawa; Moriyuki Kawauchi; Yuitsu Otsuka; Junxian Han; Daishiro Koshi; Kim Schiphof; Lucía Ramírez; Antonio G Pisabarro; Yoichi Honda

  Applied microbiology and biotechnology, 19 Feb. 2024, Peer-reviewed, Invited, Lead author

  
• Visualizing organelles with recombinant fluorescent proteins in the white-rot fungus Pleurotus ostreatus

  Kazuhiro Kurebayashi; Takehito Nakazawa; Shivani; Yuta Higashitarumizu; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Fungal Biology, 01 Oct. 2023, Peer-reviewed

  
• A Molecular Genetics Approach to Elucidating the Mechanisms Underlying Lignin Degradation by White-Rot Fungi

  Takehito Nakazawa

  Lignin, Aug. 2023, Peer-reviewed, Invited, Lead author, Last author, Corresponding author

  
• The Cas9-gRNA ribonucleoprotein complex-mediated editing of pyrG in Ganoderma lucidum and unexpected insertion of contaminated DNA fragments.

  Hyerang Eom; Yeon-Jae Choi; Rutuja Nandre; Hui-Gang Han; Sinil Kim; Minseek Kim; Youn-Lee Oh; Takehito Nakazawa; Yoichi Honda; Hyeon-Su Ro

  Scientific reports, 10 Jul. 2023, Peer-reviewed

  
• Experimental evidence that lignin-modifying enzymes are essential for degrading plant cell wall lignin by Pleurotus ostreatus using CRISPR/Cas9.

  Takehito Nakazawa; Iori Yamaguchi; Yufan Zhang; Chinami Saka; Hongli Wu; Keita Kayama; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Environmental microbiology, 22 May 2023, Peer-reviewed, Lead author

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• Physiological function of hydrophobin Vmh3 in lignin degradation by white-rot fungus Pleurotus ostreatus.

  Junxian Han; Moriyuki Kawauchi; Yuki Terauchi; Akira Yoshimi; Chihiro Tanaka; Takehito Nakazawa; Yoichi Honda

  Letters in applied microbiology, 03 Apr. 2023, Peer-reviewed

  
• Features of disruption mutants of genes encoding for hydrophobin Vmh2 and Vmh3 in mycelial formation and resistance to environmental stress in Pleurotus ostreatus.

  Junxian Han; Moriyuki Kawauchi; Kim Schiphof; Yuki Terauchi; Akira Yoshimi; Chihiro Tanaka; Takehito Nakazawa; Yoichi Honda

  FEMS microbiology letters, 17 Jan. 2023, Peer-reviewed

  
• The lignin-degrading abilities of Gelatoporia subvermispora gat1 and pex1 mutants generated via CRISPR/Cas9.

  Takehito Nakazawa; Chikako Inoue; Ryota Morimoto; Dong Xuan Nguyen; Dapeng Bao; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Environmental microbiology, 23 Mar. 2023, Peer-reviewed, Lead author, Corresponding author

  
• Double-gene targeting with pre-assembled Cas9 ribonucleoprotein for safe genome editing in the edible mushroom Pleurotus ostreatus.

  Tatpong Boontawon; Takehito Nakazawa; Yeon-Jae Choi; Hyeon-Su Ro; Minji Oh; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  FEMS microbiology letters, 22 Feb. 2023, Peer-reviewed

  
• A carnivorous mushroom paralyzes and kills nematodes via a volatile ketone.

  Ching-Han Lee; Yi-Yun Lee; Yu-Chu Chang; Wen-Li Pon; Sue-Ping Lee; Niaz Wali; Takehito Nakazawa; Yoichi Honda; Jiun-Jie Shie; Yen-Ping Hsueh

  Science advances, 18 Jan. 2023, Peer-reviewed

  
• Introducing multiple-gene mutations in Pleurotus ostreatus using a polycistronic tRNA and CRISPR guide RNA strategy.

  Haibo Xu; Takehito Nakazawa; Yufan Zhang; Minji Oh; Dapeng Bao; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  FEMS microbiology letters, 09 Nov. 2022, Peer-reviewed

  
• Coprinopsis cinerea dioxygenase is an oxygenase forming 10(S)-hydroperoxide of linoleic acid, essential for mushroom alcohol, 1-octen-3-ol synthesis.

  Takuya Teshima; Risa Funai; Takehito Nakazawa; Junya Ito; Toshihiko Utsumi; Pattana Kakumyan; Hiromi Mukai; Toyoshi Yoshiga; Ryutaro Murakami; Kiyotaka Nakagawa; Yoichi Honda; Kenji Matsui

  The Journal of biological chemistry, 16 Sep. 2022, Peer-reviewed

  
• Gene targeting of dikaryotic Pleurotus ostreatus nuclei using the CRISPR/Cas9 system.

  Fuga Yamasaki; Takehito Nakazawa; Minji Oh; Dapeng Bao; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  FEMS microbiology letters, 24 Aug. 2022, Peer-reviewed

  
• CRISPR/Cas9 using a transient transformation system in Ceriporiopsis subvermispora.

  Takehito Nakazawa; Chikako Inoue; Dong Xuan Nguyen; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Applied microbiology and biotechnology, 29 Jul. 2022, Peer-reviewed, Lead author

  
• Marker-free genome editing in the edible mushroom, Pleurotus ostreatus, using transient expression of genes required for CRISPR/Cas9 and for selection

  Daishiro Koshi; Hiroki Ueshima; Moriyuki Kawauchi; Takehito Nakazawa; Masahiro Sakamoto; Mana Hirata; Kosuke Izumitsu; Takuya Sumita; Toshikazu Irie; Yoichi Honda

  Journal of Wood Science, Dec. 2022, Peer-reviewed

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• Overexpressing Pleurotus ostreatus rho1b results in transcriptional upregulation of the putative cellulolytic enzyme‐encoding genes observed in ccl1 disruptants

  Nozomi Okuda; Takehito Nakazawa; Masato Horii; Hongli Wu; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Environmental Microbiology, 19 Oct. 2021, Peer-reviewed, Corresponding author

  
• Cunninghamella saisamornae (Cunninghamellaceae, Mucorales), a new soil fungus from northern Thailand

  NAKARIN SUWANNARACH; JATURONG KUMLA; CHANTANA SUPO; YOICHI HONDA; TAKEHITO NAKAZAWA; CHARTCHAI KHANONGNUCH; PAIROTE WONGPUTTISIN

  Phytotaxa, 30 Jun. 2021, Peer-reviewed

  
• Gene targeting using pre-assembled Cas9 ribonucleoprotein and split-marker recombination in Pleurotus ostreatus.

  Tatpong Boontawon; Takehito Nakazawa; Haibo Xu; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  FEMS microbiology letters, 22 Jun. 2021, Peer-reviewed

  
• Functional analyses of Pleurotus ostreatus pcc1 and clp1 using CRISPR/Cas9.

  Tatpong Boontawon; Takehito Nakazawa; Masato Horii; Masami Tsuzuki; Kawauchi Moriyuki; Masahiro Sakamoto; Yoichi Honda

  Fungal genetics and biology : FG & B, 18 Jun. 2021, Peer-reviewed, Corresponding author

  
• Efficient genome editing with CRISPR/Cas9 in Pleurotus ostreatus.

  Tatpong Boontawon; Takehito Nakazawa; Chikako Inoue; Keishi Osakabe; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  AMB Express, 20 Feb. 2021, Peer-reviewed

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• Targeted disruption of hir1 alters the transcriptional expression pattern of putative lignocellulolytic genes in the white-rot fungus Pleurotus ostreatus.

  Hongli Wu; Takehito Nakazawa; Ryota Morimoto; Shivani; Masahiro Sakamoto; Yoichi Honda

  Fungal genetics and biology : FG & B, Feb. 2021, Peer-reviewed, Corresponding author

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• Comparative transcriptional analyses of Pleurotus ostreatus mutants on beech wood and rice straw shed light on substrate-biased gene regulation.

  Hongli Wu; Takehito Nakazawa; Haibo Xu; Ruiheng Yang; Dapeng Bao; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Applied microbiology and biotechnology, Feb. 2021, Peer-reviewed, Corresponding author

  
• Molecular breeding of sporeless strains of Pleurotus ostreatus using a non-homologous DNA end-joining defective strain

  Fuga Yamasaki; Takehito Nakazawa; Masahiro Sakamoto; Yoichi Honda

  Mycological Progress, Jan. 2021, Peer-reviewed

  
• Filamentous fungi with high paraquat‐degrading activity isolated from contaminated agricultural soils in northern Thailand

  P. Wongputtisin; C. Supo; N. Suwannarach; Y. Honda; T. Nakazawa; J. Kumla; S. Lumyong; C. Khanongnuch

  Letters in Applied Microbiology, 30 Dec. 2020, Peer-reviewed

  
• A promoter assay system using gene targeting in agaricomycetes Pleurotus ostreatus and Coprinopsis cinerea.

  Dong Xuan Nguyen; Takehito Nakazawa; Genki Myo; Chikako Inoue; Moriyuki Kawauchi; Masahiro Sakamoto; Yoichi Honda

  Journal of microbiological methods, Dec. 2020, Peer-reviewed

  
• Characterization of the effects of terminators and introns on recombinant gene expression in the basidiomycete Ceriporiopsis subvermispora.

  Dong Xuan Nguyen; Emi Nishisaka; Moriyuki Kawauchi; Takehito Nakazawa; Masahiro Sakamoto; Yoichi Honda

  Journal of microbiology (Seoul, Korea), Dec. 2020, Peer-reviewed

  
• Transcriptional shifts in delignification-defective mutants of the white-rot fungus Pleurotus ostreatus.

  Hongli Wu; Takehito Nakazawa; Atsuki Takenaka; Rina Kodera; Ryota Morimoto; Masahiro Sakamoto; Yoichi Honda

  FEBS letters, Oct. 2020, Peer-reviewed, Corresponding author

  
• A 14-bp stretch plays a critical role in regulating gene expression from β1-tubulin promoters of basidiomycetes.

  Dong Xuan Nguyen; Taku Sakaguchi; Takehito Nakazawa; Masahiro Sakamoto; Yoichi Honda

  Current genetics, Feb. 2020, Peer-reviewed

  
• Genomic Mushroom Hunting Decrypts Coprinoferrin, A Siderophore Secondary Metabolite Vital to Fungal Cell Development.

  Yuta Tsunematsu; Jun Takanishi; Shihori Asai; Takahiro Masuya; Takehito Nakazawa; Kenji Watanabe

  Organic letters, 20 Sep. 2019, Peer-reviewed

  
• Dominant effects of gat1 mutations on the ligninolytic activity of the white-rot fungus Pleurotus ostreatus.

  Takehito Nakazawa; Ryota Morimoto; Hongli Wu; Rina Kodera; Masahiro Sakamoto; Yoichi Honda

  Fungal biology, Mar. 2019, Peer-reviewed, Lead author, Corresponding author

  
• Biosynthesis of lagopodins in mushroom involves a complex network of oxidation reactions.

  Takahiro Masuya; Yuta Tsunematsu; Yuichiro Hirayama; Michio Sato; Hiroshi Noguchi; Takehito Nakazawa; Kenji Watanabe

  Organic & biomolecular chemistry, 02 Jan. 2019, Peer-reviewed

  
• Efficient transformation of Pleurotus eryngii with a safe selective marker mutated from the Pesdi1 gene.

  Junjun Shang; Yan Li; Ruiheng Yang; Ying Wang; Wenjun Mao; Lihua Tang; Yingying Wu; Takehito Nakazawa; Yoichi Honda; Yan Li; Dapeng Bao

  Journal of microbiological methods, Sep. 2018, Peer-reviewed

  
• Effects of pex1 disruption on wood lignin biodegradation, fruiting development and the utilization of carbon sources in the white-rot Agaricomycete Pleurotus ostreatus and non-wood decaying Coprinopsis cinerea.

  Takehito Nakazawa; Ayako Izuno; Masato Horii; Rina Kodera; Hiroshi Nishimura; Yuichiro Hirayama; Yuta Tsunematsu; Yasumasa Miyazaki; Tatsuya Awano; Hajime Muraguchi; Kenji Watanabe; Masahiro Sakamoto; Keiji Takabe; Takashi Watanabe; Yuji Isagi; Yoichi Honda

  Fungal genetics and biology : FG & B, Dec. 2017, Peer-reviewed, Lead author, Corresponding author

  
• Identification of two mutations that cause defects in the ligninolytic system through an efficient forward genetics in the white-rot agaricomycete Pleurotus ostreatus.

  Takehito Nakazawa; Ayako Izuno; Rina Kodera; Yasumasa Miyazaki; Masahiro Sakamoto; Yuji Isagi; Yoichi Honda

  Environmental microbiology, Jan. 2017, Peer-reviewed, Lead author, Corresponding author

  
• Marker recycling via 5-fluoroorotic acid and 5-fluorocytosine counter-selection in the white-rot agaricomycete Pleurotus ostreatus.

  Takehito Nakazawa; Masami Tsuzuki; Toshikazu Irie; Masahiro Sakamoto; Yoichi Honda

  Fungal biology, Sep. 2016, Peer-reviewed, Lead author, Corresponding author

  
• A mutation in the Cc.arp9 gene encoding a putative actin-related protein causes defects in fruiting initiation and asexual development in the agaricomycete Coprinopsis cinerea.

  Takehito Nakazawa; Yuki Ando; Takeshi Hata; Kiyoshi Nakahori

  Current genetics, Aug. 2016, Peer-reviewed, Lead author, Corresponding author

  
• Absence of a gene encoding cytosine deaminase in the genome of the agaricomycete Coprinopsis cinerea enables simple marker recycling through 5-fluorocytosine counterselection.

  Takehito Nakazawa; Yoichi Honda

  FEMS microbiology letters, Aug. 2015, Peer-reviewed, Lead author, Corresponding author

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• Targeted disruption of transcriptional regulators in Chaetomium globosum activates biosynthetic pathways and reveals transcriptional regulator-like behavior of aureonitol.

  Takehito Nakazawa; Kan'ichiro Ishiuchi; Michio Sato; Yuta Tsunematsu; Satoru Sugimoto; Yasutaka Gotanda; Hiroshi Noguchi; Kinya Hotta; Kenji Watanabe

  Journal of the American Chemical Society, 11 Sep. 2013, Peer-reviewed, Lead author

  
• Echinomycin biosynthesis.

  Michio Sato; Takehito Nakazawa; Yuta Tsunematsu; Kinya Hotta; Kenji Watanabe

  Current opinion in chemical biology, Aug. 2013, Peer-reviewed

  
• Combinatorial generation of complexity by redox enzymes in the chaetoglobosin A biosynthesis.

  Kan'ichiro Ishiuchi; Takehito Nakazawa; Fumitoshi Yagishita; Takashi Mino; Hiroshi Noguchi; Kinya Hotta; Kenji Watanabe

  Journal of the American Chemical Society, 15 May 2013, Peer-reviewed

  
• Cc.snf5, a gene encoding a putative component of the SWI/SNF chromatin remodeling complex, is essential for sexual development in the agaricomycete Coprinopsis cinerea

  Yuki Ando; Takehito Nakazawa; Kunihiko Oka; Kiyoshi Nakahori; Takashi Kamada

  FUNGAL GENETICS AND BIOLOGY, Jan. 2013, Peer-reviewed

  
• Overexpressing transcriptional regulator in Chaetomium globosum activates a silent biosynthetic pathway: evaluation of shanorellin biosynthesis

  Yuta Tsunematsu; Suguru Ichinoseki; Takehito Nakazawa; Noriyasu Ishikawa; Hiroshi Noguchi; Kinya Hotta; Kenji Watanabe

  JOURNAL OF ANTIBIOTICS, Jul. 2012, Peer-reviewed

  
• Establishing a New Methodology for Genome Mining and Biosynthesis of Polyketides and Peptides through Yeast Molecular Genetics

  Kan'ichiro Ishiuchi; Takehito Nakazawa; Takashi Ookuma; Satoru Sugimoto; Michio Sato; Yuta Tsunematsu; Noriyasu Ishikawa; Hiroshi Noguchi; Kinya Hotta; Hisao Moriya; Kenji Watanabe

  CHEMBIOCHEM, Apr. 2012, Peer-reviewed

  
• Overexpressing Transcriptional Regulator in Aspergillus oryzae Activates a Silent Biosynthetic Pathway to Produce a Novel Polyketide

  Takehito Nakazawa; Kan'ichiro Ishiuchi; Alex Praseuth; Hiroshi Noguchi; Kinya Hotta; Kenji Watanabe

  CHEMBIOCHEM, Apr. 2012, Peer-reviewed, Lead author

  
• 生合成遺伝子クラスターの高度強制発現による合成生物学が拓く有用天然物の創製

  中沢威人; 恒松雄太; 石川格靖; 渡辺賢二

  生物工学会誌: seibutsu-kogaku kaishi, 2012, Peer-reviewed, Lead author

  
• Efficient gene targeting in Delta Cc.ku70 or Delta Cc.lig4 mutants of the agaricomycete Coprinopsis cinerea

  Takehito Nakazawa; Yuki Ando; Kohei Kitaaki; Kiyoshi Nakahori; Takashi Kamada

  FUNGAL GENETICS AND BIOLOGY, Oct. 2011, Peer-reviewed, Lead author

  
• P-72 Establishing a New Methodology for Genome Mining and Biosynthesis of Natural Products Through a Yeast Molecular Genomics(Poster Presentation)

  Ishiuchi Kan'ichiro; Nakazawa Takehito; Ookuma Takashi; Gotanda Yasutaka; Noguchi Hiroshi; Moriya Hisao; Watanabe Kenji

  Symposium on the Chemistry of Natural Products, symposium papers, 02 Sep. 2011

  
• A mutation in the Cc.ubc2 gene affects clamp cell morphogenesis as well as nuclear migration for dikaryosis in Coprinopsis cinerea

  Takehito Nakazawa; Hirofumi Kondo; Kiyoshi Nakahori; Takashi Kamada

  FUNGAL GENETICS AND BIOLOGY, May 2011, Peer-reviewed, Lead author

  
• Regulation of fruiting body photomorphogenesis in Coprinopsis cinerea

  Takashi Kamada; Hiroaki Sano; Takehito Nakazawa; Kiyoshi Nakahori

  FUNGAL GENETICS AND BIOLOGY, Nov. 2010, Peer-reviewed

  
• Mutations in the Cc.rmt1 gene encoding a putative protein arginine methyltransferase alter developmental programs in the basidiomycete Coprinopsis cinerea

  Takehito Nakazawa; Yoshiaki Tatsuta; Takashi Fujita; Kiyoshi Nakahori; Takashi Kamada

  CURRENT GENETICS, Aug. 2010, Peer-reviewed, Lead author

  
• The dst2 gene essential for photomorphogenesis of Coprinopsis cinerea encodes a protein with a putative FAD-binding-4 domain

  Masaki Kuratani; Kanako Tanaka; Kazuhisa Terashima; Hajime Muraguchi; Takehito Nakazawa; Kiyoshi Nakahori; Takashi Kamada

  FUNGAL GENETICS AND BIOLOGY, Feb. 2010, Peer-reviewed

  
• The homologue of Lentinula edodes ctg1, a target for CDC5 and its interacting partner CIPB, from Coprinopsis cinerea is involved in fruiting-body morphogenesis of C. Cinerea

  Takehito Nakazawa; Shinya Kaneko; Hitoshi Murata; Takashi Kamada; Kazuo Shishido

  MYCOSCIENCE, Sep. 2009, Peer-reviewed, Lead author, Corresponding author

  
• Stimulative effects of light and a temperature downshift on transcriptional expressions of developmentally regulated genes in the initial stages of fruiting-body formation of the basidiomycetous mushroom Lentinula edodes

  Takehito Nakazawa; Yasumasa Miyazaki; Shinya Kaneko; Kazuo Shishido

  FEMS MICROBIOLOGY LETTERS, Dec. 2008, Peer-reviewed, Lead author, Corresponding author

  
• Basidiomycete Lentinula edodes CDC5 and a novel interacting protein CIPB bind to a newly isolated target gene in an unusual manner

  Takehito Nakazawa; Shinya Kaneko; Yasumasa Miyazaki; Toru Jojima; Takashi Yamazaki; Shiho Katsukawa; Kazuo Shishido

  FUNGAL GENETICS AND BIOLOGY, Jun. 2008, Peer-reviewed, Lead author

  
• Developmental regulator Le.CDC5 of the mushroom Lentinula edodes: analyses of its amount in each of the stages of fruiting-body formation and its distribution in parts of the fruiting bodies

  T Nakazawa; Y Miyazaki; S Kaneko; K Shishido

  FEMS MICROBIOLOGY LETTERS, Aug. 2006, Peer-reviewed, Lead author

  

Misc.

• Development of a high-density liquid culture strain of the white-rot fungus Pleurotus ostreatus

  大塚唯; 河内護之; 辻健也; 吉見啓; 吉見啓; 田中千尋; 田中千尋; 矢野成和; 入江俊一; 中沢威人; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2024

  
• Developing a protocol for mycelial material production using the white-rot fungus Pleurotus ostreatus

  小島隼星; KIM Schiphof; 河内護之; 辻健也; 田中千尋; 田中千尋; JEONG Yong-Hyeon; SHIN Hyun-Jae; 中沢威人; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2024

  
• Functional analysis of highly expressed hydrophobin hydph16 during wood degradation in the white-rot fungus Pleurotus ostreatus

  HAN Junxian; 河内護之; 辻健也; 寺内裕貴; 吉見啓; 吉見啓; 田中千尋; 田中千尋; 中沢威人; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2024

  
• Functional analysis of the transcription factor Swi6, which contributes to the regulation of cell wall synthesis in the white-rot fungus, Pleurotus ostreatus.

  和泉貴哉; 小島隼星; 大塚唯; 河内護之; 辻健也; 吉見啓; 田中千尋; 田中千尋; 矢野成和; 中沢威人; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2024

  
• Analysis of physiological functions of the β-glucan synthase gene of Pleurotus ostreatus for elucidation of cell wall structure of white-rot fungi

  河内護之; 名和義順; 大塚唯; KIM Schiphof; WAN Y.; 辻健也; 吉見啓; 吉見啓; 田中千尋; 田中千尋; 中沢威人; 矢野成和; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2024

  
• 細胞壁多糖三重染色法の開発により見えてきた木材腐朽菌にユニークな細胞壁構造

  大塚唯; 河内護之; 辻健也; 吉見啓; 田中千尋; 中沢威人; 矢野成和; 本田与一

  応用糖質科学, 2024

  
• Basidiomycete dioxygenase is essential for forming 1-octen-3-ol, a major volatile of mushrooms

  手嶋琢; 中沢威人; 内海俊彦; 伊藤隼哉; 向井裕美; 仲川清隆; 本田与一; 松井健二

  日本きのこ学会大会講演要旨集, 2022

  
• キノコ主要香気成分に寄与する担子菌ジオキシゲナーゼの機能解析

  手嶋琢; 中沢威人; 内海俊彦; 伊藤隼哉; 仲川清隆; 本田与一; 松井健二

  日本農芸化学会中四国支部講演会講演要旨集(Web), 2022

  
• Characterization of cell wall synthesis regulatory transcription factors Mbp1 and Rlm1 in basidiomycete Pleurotus ostreatus

  小島隼星; 亀井志乃; 名和義順; 大塚唯; 河内護之; 寺内裕貴; 吉見啓; 田中千尋; 矢野成和; 中沢威人; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2023

  
• Analysis of xylan synthase genes in grasses

  坂本正弘; 長谷川智彦; 河内護之; 中沢威人; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2023

  
• 細胞壁多糖三重染色法の開発により見えてきた木材腐朽菌にユニークな細胞壁構造

  大塚唯; 河内護之; 辻健也; 吉見啓; 田中千尋; 中沢威人; 矢野成和; 本田与一

  応用糖質科学, 2023

  
• Unique cell wall structure of basidiomycete focused on α-1,3-glucan in Pleurotus ostreatus

  河内護之; 名和義順; 大塚唯; 寺内裕貴; 吉見啓; 田中千尋; 阿部啓悦; 代田修; 矢野成和; 中沢威人; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2023

  
• 木材腐朽菌の細胞壁合成機構の理解を目的としたグルカン合成酵素の解析

  河内護之; 名和義則; 大塚唯; 辻健也; 吉見啓; 田中千尋; 阿部啓悦; 矢野成和; 中沢威人; 本田与一

  応用糖質科学, 2023

  
• Identification and functional analysis of β-glucan synthase genes in Pleurotus ostreatus

  名和義順; 大塚唯; 河内護之; 寺内裕貴; 吉見啓; 田中千尋; 矢野成和; 中沢威人; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2023

  
• Isolation of genes essential for basidiospore production in Pleurotus ostreatus using transcriptome analysis and CRISPR/Cas9

  小深田剛士; 山崎風雅; 中沢威人; 菅野純子; OH Minji; 河内護之; 坂本正弘; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2023

  
• Histone H3K36 methylation affects indirectly transcriptional alternation of cellulose degrading enzyme genes in Pleurotus ostreatus

  奥田希実; 中沢威人; 香山渓太; 河内護之; 坂本正弘; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2022

  
• A marker-free genome editing technique via transient gene expression in an edible mushroom, Pleurotus ostreatus.

  越大志朗; 河内護之; 入江俊一; 中沢威人; 坂本正弘; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2022

  
• Function of an autonomously replicating sequence AMA1 in the basidiomycete Pleurotus ostreatus and its application to genome editing

  越大志朗; 河内護之; 中沢威人; 坂本正弘; 本田与一

  日本農芸化学会大会講演要旨集(Web), 2022

  
• Identification and functional analysis of α-1,3-glucan synthase gene in Pleurotus ostreatus

  名和義順; 河内護之; 中沢威人; 坂本正弘; 阿部敬悦; 田中千尋; 吉見啓; 本田与一

  日本農芸化学会大会講演要旨集(Web), 2022

  
• Fluorescence imaging visualizes the distinct cell wall structures of Basidiomycetes and Ascomycetes

  大塚唯; 山田夕月; 辻健也; 河内護之; 中沢威人; 吉見啓; 田中千尋; 坂本正弘; 矢野成和; 本田与一

  日本分子生物学会年会プログラム・要旨集(Web), 2022

  
• Roles of CCAAT-boxes in transcription of ligninolytic enzyme genes in Pleurotus ostreatus on lignocellulose

  中沢威人; 坂知奈美; 山本太一; 河内護之; 坂本正弘; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 2021

  
• A transient transformation and its application to gneome editing system in pleurotus ostreatus

  越大志朗; 河内護之; 入江俊一; 中沢威人; 坂本正弘; 本田与一

  日本分子生物学会年会プログラム・要旨集(Web), 2021

  
• 担子菌シイタケの子実体形成関連タンパク質Le.CDC5に関する研究(1)子実体形成過程各段階における存在量と子実体内分布の解析

  中沢威人; 宮崎安将; 金子真也; 山崎丘; 宍戸和夫

  日本菌学会大会講演要旨集, 2006

  
• Functional analysis of the vegetative mycelium hydrophobin Vmh3 in the white rot fungus Pleurotus ostreatus

  HAN J.; 河内護之; 寺内裕貴; 吉見啓; 田中千尋; 中沢威人; 坂本正弘; 本田与一

  日本菌学会大会講演要旨集, 2022

  
• A 14-bp stretch plays a critical role in regulating gene expression from beta(1)-tubulin promoters of basidiomycetes (vol 66, pg 217, 2019)

  Dong Xuan Nguyen; Taku Sakaguchi; Takehito Nakazawa; Masahiro Sakamoto; Yoichi Honda

  CURRENT GENETICS, Apr. 2020

  
• Correction to: A 14-bp stretch plays a critical role in regulating gene expression from β1-tubulin promoters of basidiomycetes.

  Dong Xuan Nguyen; Taku Sakaguchi; Takehito Nakazawa; Masahiro Sakamoto; Yoichi Honda

  Current genetics, Apr. 2020

  
• ヒラタケにおけるmsh4およびmer3遺伝子の破壊が胞子形成に与える影響の解析

  山崎風雅; 中沢威人; 坂本正弘; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 04 Mar. 2019

  
• ヒラタケにおけるhirA遺伝子の破壊がリグニン分解能および木質分解酵素遺伝子群の転写に与える影響

  中沢威人; 森本亮太; 呉紅麗; SHIVANI; 坂本正弘; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 04 Mar. 2019

  
• イネのGUX,XAT‐RNAi抑制株の作成と細胞壁成分の解析

  長谷川智彦; 宮本託志; 山村正臣; 中沢威人; 本田与一; 梅澤俊明; 梅澤俊明; 坂本正弘

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 04 Mar. 2019

  
• Ceriporiopsis subvermisporaにおけるCRISPR/Cas9によって単離されたゲノム編集株の変異部位の解析

  井上智香子; 中沢威人; NGUYEN Dong Xuan; 刑部敬史; 坂本正弘; 本田与一

  日本きのこ学会大会講演要旨集, 2019

  
• 白色腐朽菌ヒラタケにおけるvp2, vp3遺伝子の単独および二重破壊が木粉中のリグニン分解に及ぼす影響

  坂知奈美; 中沢威人; 山田美紗登; 西村裕志; 竹中敦紀; 渡辺隆司; 坂本正弘; 本田与一

  糸状菌分子生物学コンファレンス（長岡）, Nov. 2018

  
• 白色腐朽菌ヒラタケにおけるxyn10およびxyn11遺伝子の破壊が稲わら中のリグニン分解に与える影響

  湯村直樹; 中沢威人; WU Hongli; 坂本正弘; 本田与一

  リグニン討論会講演集, 17 Oct. 2018

  
• 白色腐朽菌ヒラタケにおけるリグニン分解不全変異株の原因変異遺伝子gat1及びhirAの同定

  森本亮太; 中沢威人; 坂本正弘; 本田与一

  リグニン討論会講演集, 17 Oct. 2018

  
• イネのGUX,XAT‐RNAi抑制株の作成と細胞壁構成成分の解析

  長谷川智彦; 中沢威人; 本田与一; 坂本正弘

  リグニン討論会講演集, 17 Oct. 2018

  
• ヒラタケにおける分泌型レポーターアッセイシステムの構築

  坂知奈美; 中沢威人; 湯村直樹; 竹中敦紀; NGUYEN Dong Xuan; 坂本正弘; 本田与一

  日本きのこ学会大会講演要旨集, 31 Aug. 2018

  
• ヒラタケにおけるdst2遺伝子の破壊が子実体形成に与える影響の解析

  堀井雅人; 中沢威人; 坂本正弘; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 05 Mar. 2018

  
• ヒラタケにおけるリグニン分解不全変異が,多糖分解関連酵素遺伝子群の転写に与える影響の解析

  中沢威人; 小寺里奈; 竹中敦紀; 森本亮太; 坂本正弘; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 05 Mar. 2018

  
• 白色腐朽菌ヒラタケおよびCeriporiopsis subvermisporaにおけるCRISPR/Cas9を用いたゲノム編集系の確立

  井上智香子; 中沢威人; NGUYEN Dong Xuan; 刑部敬史; 坂本正弘; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 05 Mar. 2018

  
• イネの茎におけるGUX,XATの成長段階における発現量解析

  長谷川智彦; 中沢威人; 本田与一; 坂本正弘

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 05 Mar. 2018

  
• Recent progress in molecular genetic approaches for mushroom science

  HONDA Yoichi; NAKAZAWA Takehito

  Satellite workshop of 14th European Conference on Fungal Genetics, Feb. 2018

  
• Efficient forward genetics studies to identify mutations that cause defects in the ligninolytic activity in the white-rot Agaricomycete Pleurotus ostreatus

  NAKAZAWA Takehito; HONDA Yoichi

  Satellite workshop of 14th European Conference on Fungal Genetics, Feb. 2018

  
• Functional analysis of gene expression signals in basidiomycetous fungi using transfection, and random or targeted integration on the chromosome

  Yoichi HONDA; Dong X NGUYEN; Emi NISHISAKA; Taku SAKAGUCHI; Takehito NAKAZAWA; Masahiro SAKAMOTO

  14th European Conference on Fungal Genetics, Feb. 2018

  
• Cellulolytic enzyme genes are significantly upregulated by most of the mutations that cause defects in the ligninolytic activity in the white-rot fungus Pleurotus ostreatus

  Takehito NAKAZAWA; Rina KODERA; Ryota MORIMOTO; Atsuki TAKENAKA; Shoko TSUJI; Hiroshi NISHIMURA; Takashi WATANABE; Masahiro SAKAMOTO; Yoichi HONDA

  14th European Conference on Fungal Genetics, Feb. 2018

  
• Identification of cis-acting elements in the glyceraldehyde-3-phosphate dehydrogenase terminator of Ceriporiopsis subvermispora

  Dong X. Nguyen; Emi Nishisaka; Takehito Nakazawa; Masahiro Sakamoto; Yoichi Honda

  第17回糸状菌分子生物学コンファレンス, Nov. 2017

  
• 白色腐朽菌ヒラタケにおけるGH10・GH11の機能喪失がリグニン分解に及ぼす影響について

  湯村直樹; 中沢威人; 大沼広宜; 泉津弘佑; 入江俊一; 福田泰久; 白坂憲章; 坂本正弘; 本田与一

  リグニン討論会講演集, 13 Oct. 2017

  
• A 14-nt stretch plays a significant role in gene expression from beta-tubulin promoter in basidiomycetes

  Dong X Nguyen; Taku Sakaguchi; Takehito Nakazawa; Masahiro Sakamoto; Yoichi Honda

  Asia Mycological Congress, Oct. 2017

  
• キノコによる木質リグニン分解と有性形態形成の間に、関係性はあるのか？

  中沢威人; 堀井雅人; 森本亮太; 辻祥子; 西村裕志; 小寺里奈; 坂本正弘; 渡辺隆司; 本田与一

  62nd Lignin symp., Nagoya, Oct. 2017

  
• Removal of some allergic proteins in natural rubber latex by using Bacillus sp. proteases

  Pairote Wongputtisin; Chartchai Khanongnuch; Augchararat Klongklaew Honda; Takehito Nakazawa

  The 7th International conference on fermentation technology for value added agricultural products, Jul. 2017, Invited

  
• Fungal strains with degradation activity of paraquat isolated from Northern Thailand

  Yoichi Honda; Pairote Wongputtisin; Chantana Supo; Takehito Nakazawa; Nakarin Suwannarach; Saisamorn Lumyong; Chartchai Khanongnuch

  The 7th International conference on fermentation technology for value added agricultural products, Jul. 2017, Invited

  
• 担子菌ヒトヨタケおよびヒラタケにおけるpex1破壊株の表現型の共通点と相違点

  中沢威人; 本田与一

  日本農芸化学会大会講演要旨集(Web), 05 Mar. 2017

  
• ヘミセルロース合成阻害による細胞壁構造への影響〜リグニン構造への影響〜

  真嶋雄二郎; 加藤あすか; 中沢威人; 本田与一; 山村正臣; 飛松裕基; 梅澤俊明; 坂本正弘

  第67回日本木材学会大会, Mar. 2017

  
• 白色腐朽菌ヒラタケにおけるpex1遺伝子の機能喪失が、木質生分解および子実体発生に与える影響

  中沢威人; 伊津野彩子; 井鷺裕司; 粟野達也; 高部圭司; 宮崎安将; 村口 元; 西村裕志; 渡辺隆司; 本田与一

  日本木材学会大会（福岡大会）Oral, Mar. 2017

  
• Isolation of soil bacteriacapable of paraquat degradation

  C. Supo; P. Wongputtisin; M.K. Pholcham; J. Inthasam; Y. Honda; T. Nakazawa; C. Khanongnuch

  The 28th annual meeting of the Thai society for biotechnology and international conference, Nov. 2016

  
• Factors involving in paraquat degradation ability by the effective microorganisms

  Pairote Wongputtisin; Chantana Supo; Yoichi Honda; Takehito Nakazawa; Nakarin Suwannarach; Saisamorn Lumyong; Chartchai Khanongnuch

  The 2nd Joint Seminar, Core to Core Program A Advanced Research Networks, Nov. 2016

  
• 順遺伝学アプローチを起点とした,リグニン分解酵素活性およびリグニン生分解に重要な新たな因子の探索

  中沢威人; 小寺里奈; 西村裕志; 坂本正弘; 渡辺隆司; 本田与一

  リグニン討論会講演集, 14 Oct. 2016

  
• ヘミセルロースの合成阻害による細胞壁成分への影響

  真嶋雄二郎; 加藤あすか; 中沢威人; 本田与一; 山村正臣; 飛松裕基; 梅澤俊明; 坂本正弘

  第61回リグニン討論会, Oct. 2016

  
• ヒラタケを用いた真正担子菌類における有性形態形成に関わる分子機構の保存性の検証

  堀井雅人; 中沢威人; 都築雅美; 坂本正弘; 本田与一

  日本菌学会大会講演要旨集, Sep. 2016

  
• ヒラタケを用いた順遺伝学実験系の確立:白色腐朽に重要な新しい因子の同定に向けて

  中沢威人; 伊津野彩子; 宮崎安将; 井鷺裕司; 本田与一

  日本木材学会大会研究発表要旨集(完全版)(CD-ROM), Mar. 2016

  
• Effect of 1,1’- dimethyl - 4,4’- dipyridylium dichloride on bacterial community and enzyme activities of soils from Para rubber plantation area

  Pairote Wongputtisin; Yoichi Honda; Takehito Nakazawa; Jiraporn Inthas; Chartchai Khanongnuch

  The 6th International Conference on Fermentation Technology for Value Added Agricultural Products, Jul. 2015

  
• チュウゴクザサのFDタンパク質は花芽分裂組織決定遺伝子のプロモーター領域に結合する

  長田充洋; 中沢威人; 本田与一; 坂本正弘

  日本植物細胞分子生物学会大会・シンポジウム講演要旨集, Jul. 2015

  
• Forward Genetics in the white-rot fungus Pleurotus ostreatus: Towards identifyion of moleculer mechanisms essentiall for ligninytic system

  Nakazawa T; Izuno A; Miyazaki Y; Isagi Y; Honda Y

  28th Fungal Genetics Conference, Mar. 2015, Peer-reviewed

  
• UDP-グルクロン酸脱炭酸酵素(UXS)遺伝子の発現抑制による 細胞壁成分の変化

  下里知沙; 加藤あすか; 中沢威人; 本田与一; 山村正臣; 梅澤俊明; 坂本正弘

  第65回 日本木材学会、東京, Mar. 2015

  
• Biotechnological application as a sustainable approach for improvement of rubber production in northern Thailand

  Pairote Wongputtisin; Nareeluk Nakaew; Augchararat Klongkreaw; Takehito Nakazawa; Yoichi Honda; Chartchai Khanongnuch

  The 1st Joint Seminar forNew Core to Core Program A. Advanced Research Networks on Establishment of an international research core for new bio-research fields with microbes from tropical areas (World-class research hub of tropical microbial resource, Nov. 2014

  
• Analysis of N-glycan of the extracellular versatile peroxidase MnP2 secreted by Pleurotus ostreatus

  Keigo ITO; Jun NAGAOKA; Koichi YOSHIOKA; Takehito NAKAZAWA; Masahiro SAKAMOTO; Takashi WATANABE; Yoichi HONDA

  8th International Conference on Mushroom Bioloby and Mushroom Products, Nov. 2014, Peer-reviewed

  
• 白色腐朽菌によるリグニン生分解に必須な遺伝子の同定にむけた基盤整備

  中沢威人; 宮崎安将; 本田与一

  第１４回糸状菌分子生物学カンファレンス, Nov. 2014, Invited

  
• ヒラタケの野生型MnP2の糖鎖構造解析

  伊藤奎梧; 長岡潤; 吉岡康一; 中沢威人; 坂本正弘; 渡辺隆司; 本田与一

  日本きのこ学会25周年記念大会, 京都, 11 Sep. 2014

  
• 担子菌Ceriporiopsis subvermisporaにおけるβ‐tubulin遺伝子のプロモーター解析(第二報)

  SAKAGUCHI TAKU; NAKAZAWA TAKEHITO; SAKAMOTO MASAHIRO; HONDA YOICHI

  日本きのこ学会大会講演要旨集, 01 Sep. 2014

  
• 木材腐朽菌によるメラニン脱色機構の解析

  NUMATA MOTOSHI; MINAMI MASAHIKO; SAITO YOTARO; NAKAZAWA TAKEHITO; SAKAMOTO MASAHIRO; KOIKE KENZO; HONDA YOICHI

  日本きのこ学会大会講演要旨集, 01 Sep. 2014

  
• ヒラタケにおける分泌型レポーターシステムの開発

  上田真広; 四辻諒平; 坂口拓; 中沢威人; 坂本正弘; 由里本博也; 阪井康能; 本田与一

  日本きのこ学会大会講演要旨集, 01 Sep. 2014

  
• チュウゴクザサFDホモログのイネにおける異種発現,及び組換えタンパク質の解析

  OSADA MITSUHIRO; NAKAZAWA TAKEHITO; HONDA YOICHI; SAKAMOTO MASAHIRO

  日本植物細胞分子生物学会大会・シンポジウム講演要旨集, Jul. 2014

  
• チュウゴクザサ花成制御遺伝子COホモログの解析

  YASUI NANAKO; NAKAZAWA TAKEHITO; HONDA YOICHI; SAKAMOTO MASAHIRO

  日本植物細胞分子生物学会大会・シンポジウム講演要旨集, Jul. 2014

  
• 担子菌Ceriporiopsis subvermisporaにおけるβ‐tubulin遺伝子のプロモーター解析

  SAKAGUCHI TAKU; NAKAZAWA TAKEHITO; SAKAMOTO MASAHIRO; HONDA YOICHI

  日本菌学会大会講演要旨集, May 2014

  
• 担子菌を用いたメラニン脱色機構の解析

  NUMATA MOTOSHI; MINAMI MASAHIKO; SAITO YOTARO; NAKAZAWA TAKEHITO; KOIKE KENZO; HONDA YOICHI

  日本農芸化学会大会講演要旨集(Web), Mar. 2014

  
• 白色腐朽菌の生産する糖タンパク質のN-結合型糖鎖と 外来タンパク質の修飾

  長岡 潤; 伊藤 奎吾; 中沢 威人; 坂本 正弘; 本田 与一; 吉岡 康一; 渡辺 隆司

  微生物科学研究の多様性と新展開, Nov. 2013

  
• トランスフェクションを用いた担子菌 Ceriporiopsis subvermisporaにおけるプロモーター解析

  坂口拓; 中沢威人; 坂本正弘; 谷川瑛二; 渡邊崇人; 渡辺隆司; 本田与一

  微生物科学研究の多様性と新展開, Nov. 2013

  

Presentations

• Insights form Molecular Genetics Studies in White-Rot Fungi

  Takehito Nakazawa

  Gordon Research Conference, lignin, 18 Jul. 2024, Invited

  
• 担子菌の研究：オリジナリティとマニアックの狭間で

  中沢 威人

  第９回糸状菌分子生物学研究会若手の会WS, 10 Nov. 2021, Invited

  
• 菌類遺伝学からのアプローチによるリグニン分解研究

  中沢 威人

  リグニン学会若手の会 日本木材学会バイオマス変換研究会 合同講演会, 05 Nov. 2021, Invited

  
• 担子菌キノコの特殊能力：子実体形成と木材腐朽

  中沢 威人

  第7回生物機能開発研究所講演会, 16 Jan. 2017, Invited

  
• Fungal Geneticsを軸とした異分野研究

  中沢 威人

  第二回糸状菌分子生物学研究会若手の会ワークショップ, 14 Nov. 2014, Invited

  

Books and Other Publications

• 生合成遺伝子クラスターの高度強制発現による合成生物学が拓く有用天然物の創製

  中沢威人; 恒松雄太; 石川格靖; 渡辺賢二

  2012, Not refereed

  

Awards

• 09 Mar. 2023

  16th European Conference on Fungal Genetics Best Poster Award
• 03 Sep. 2022

  リグニン学会奨励賞
• リグニン学会, 奨励賞

  白色腐朽菌ヒラタケによるリグニン分解の分子遺伝学研究, Japan, International academic award

  中沢　威人
• 17 Jun. 2022

  糸状菌遺伝子研究会奨励賞
• 糸状菌遺伝子研究会, 奨励賞

  Japan, Japan society
• 28 Feb. 2018

  European Conference on Fungal Genetics 14, Best Poster Award
• 01 Aug. 2012

  株式会社リバネス（ロート製薬）, リバネス・ヘルスケア賞
• 28 Feb. 2018

  European Conference on Fungal Genetics 14, Best Poster Award

External funds: Kakenhi

• Mycelial factory for production of biopharmaceuticals using synthetic biology

  Grant-in-Aid for Challenging Research (Pioneering)

  Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields

  Kyoto University

  本田 与一

  From 09 Jul. 2021, To 31 Mar. 2026, Granted

  白色腐朽菌;タンパク質分泌系;タンパク質分泌;ゲノム編集;糖鎖修飾;ゴルジ体

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K18224/
• Frontier studies on wood decaying fungi in the keyword "epigenetics"

  Grant-in-Aid for Scientific Research (B)

  Basic Section 40020:Wood science-related

  Kyoto University

  Takehito Nakazawa

  From 01 Apr. 2019, To 31 Mar. 2022, Project Closed

  木材腐朽菌;リグニン;セルロース;ヒストン;糸状菌;バイオマス;腐朽菌;担子菌;セルラーゼ;木材;きのこ;エピジェネティック;植物細胞壁;転写制御;キノコ;木質;木材腐朽;二次代謝;天然物

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H03017/
• Surveys and mechanism elucidation of genetic pollution caused by edible mushrooms cultivation in east Asia

  Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

  Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields

  Kyoto University

  Yoichi Honda

  From 09 Oct. 2018, To 31 Mar. 2022, Project Closed

  担子菌;きのこ;遺伝子汚染;木材腐朽菌;ゲノム解析;生物多様性;持続可能農業;食用担子菌

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18KK0178/
• Exploration of factors inducing heartwood formation expressed by external environmental factors

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields

  Kyoto University

  Masahiro Sakamoto

  From 29 Jun. 2018, To 31 Mar. 2023, Project Closed

  心材形成;師管転流;ペルオキシダーゼ;プロテインキナーゼ;情報伝達;師管液;仮道管;シグナル伝達;NACドメイン転写因子;スギ;仮道管液;RNA-seq;移行材

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K19231/
• Production of low radiocesium-absorbing mushrooms based on membrane transport mechanisms

  Grant-in-Aid for Scientific Research (C)

  Basic Section 40010:Forest science-related

  Forest Research and Management Organization

  Komatsu Masabumi

  From 01 Apr. 2018, To 31 Mar. 2021, Project Closed

  放射性セシウム;きのこ;出荷制限;セシウム;菌糸体;ルビジウム;スギタケ属;カリウム;トランスポーター

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K05756/
• Modification of the protein secretory system in wood rot fungi

  Grant-in-Aid for Scientific Research (B)

  Basic Section 40020:Wood science-related

  Kyoto University

  Yoichi Honda

  From 01 Apr. 2018, To 31 Mar. 2022, Project Closed

  木材腐朽菌;リグニン分解;タンパク質分泌系;担子菌;分泌系;細胞内小器官;白色腐朽菌;タンパク質分泌;木材分解酵素;遺伝子組換え;リグニン分解酵素;分泌酵素;ゲノム編集;糖鎖修飾

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18H02254/
• Investigation of molecular mechanism for switching from vegetative growth to sexual growth in mushroom forming fungi

  Grant-in-Aid for Scientific Research (B)

  Iwate Biotechnology Research Center

  Sakamoto Yuichi

  From 01 Apr. 2017, To 31 Mar. 2020, Project Closed

  成長相転換;子実体発生誘導;光受容体;ゲノム編集;きのこ;ウシグソヒトヨタケ;担子菌類;子実体;発生誘導;栄養シグナル;脂質;子実体形成;応用微生物;発生・分化;菌類

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17H03798/
• Identification of factors important for lignin biodegradation by white-rot fungi

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  Takehito Nakazawa

  From 01 Apr. 2016, To 31 Mar. 2018, Project Closed

  リグニン;木材腐朽;キノコ;担子菌;順遺伝学;腐朽菌;白色腐朽菌;キシラナーゼ;遺伝子破壊;突然変異体;白色腐朽

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K18729/
• Platform formation for pharmaceutical glycoprotein production system in wood rot fungus

  Grant-in-Aid for Challenging Exploratory Research

  Kyoto University

  Yoichi Honda

  From 01 Apr. 2015, To 31 Mar. 2018, Project Closed

  白色腐朽菌;異種発現;糖タンパク質;遺伝子組換え;きのこ;組換え;タンパク質;糖鎖;担子菌;木材腐朽菌;タンパク質異種発現;組換え体;タンパク質発現

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K14771/
• Challenge for production of therapic glyco-peptides using gene expression system in wood rot fungi

  Grant-in-Aid for Challenging Exploratory Research

  Kyoto University

  Yoichi HONDA

  From 01 Apr. 2013, To 31 Mar. 2015, Project Closed

  木材腐朽菌;糖タンパク質;異種発現;糖鎖工学;担子菌;糖ペプチド

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25660137/
• キノコにおけるケミカル・ジェネティクス研究の開拓

  Grant-in-Aid for Challenging Exploratory Research

  University of Shizuoka

  中沢 威人

  From 01 Apr. 2013, To 31 Mar. 2014, Declined

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25660065/
• Methodology development for acquisition of a numerous number of new natural products from filamentous

  Grant-in-Aid for Research Activity Start-up

  University of Shizuoka

  Takehito NAKAZAWA

  From 01 Apr. 2010, To 31 Mar. 2013, Project Closed

  糸状菌;酵母;生合成;異種発現;二次代謝;形態形成;天然物化学;遺伝子工学;ケミカルジェネティクス

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23880025/
• Isolation of a safe and eco-friendly cultivar in Lentinula edodes by developing new genome editing technologies

  Grant-in-Aid for Scientific Research (A)

  Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields

  Kyoto University

  本田 与一

  From 01 Apr. 2022, To 31 Mar. 2026, Granted

  きのこ;食用担子菌;胞子;遺伝子汚染;ゲノム編集;キノコ;木材腐朽菌

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H00380/
• 白色腐朽菌に共通した転写調節機構の基礎研究と応用への道筋

  Grant-in-Aid for Scientific Research (C)

  Basic Section 40020:Wood science-related

  Kyoto University

  中沢 威人

  From 01 Apr. 2024, To 31 Mar. 2027, Granted

  白色腐朽菌;リグニン;転写因子

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K09019/

External funds: others

• 化石代替資源利活用のための白色腐朽菌の選択的リグニン分解能強化

  研究助成

  公益財団法人　ＥＮＥＯＳ東燃ゼネラル研究奨励・奨学会, 研究助成

  From 01 Apr. 2022, To 31 Mar. 2023

  中沢 威人
• 幅広い糸状菌からの休眠型二次代謝産物遺伝子クラスターの網羅的活性化および化合物生産を実現する基盤整備

  公益財団法人発酵研究所・若手研究者助成

  From 01 Apr. 2012, To 30 Jun. 2013

  中沢威人
• 菌類分子遺伝学を基盤とした、糸状菌からの新規二次代謝産物の網羅的獲

  リバネス研究費・ヘルスケア部門賞

  From 26 Jul. 2012, To 25 Jul. 2013

  中沢威人
• ヒラタケを用いた順遺伝学研究から、木質分解酵素特有の分泌機構に迫る

  公益財団法人ホクト生物科学振興財団・研究奨励金

  From 26 Nov. 2013

  中沢威人
• 白色腐朽菌の順遺伝学：担子菌特有の酸化酵素の分泌メカニズムの理解にむけて

  公益財団法人発酵研究所・一般研究助成

  From 01 Apr. 2014, To 31 Mar. 2016

  中沢威人
• 木材腐朽のシステム合成生物学への挑戦

  公益財団法人発酵研究所・大型研究助成

  From 01 Apr. 2016, To 31 Mar. 2018

  中沢　威人
• 担子菌キノコにおける、二次代謝経路とキノコ発生と木材腐朽制御の関連性

  公益財団法人野田産業科学研究所・奨励研究助成

  From 01 Apr. 2016, To 31 Mar. 2017

  中沢　威人
• 腐朽菌による木材侵襲に必須な遺伝子の同定

  公益財団法人 LIXIL住生活財団・若手研究助成

  From 01 Dec. 2017, To 01 Dec. 2018

  中沢威人
• 木材腐朽菌の進化仮説を、実験室内で実証する

  公益財団法人発酵研究所・一般研究助成

  From 01 Apr. 2018, To 31 Mar. 2020

  中沢威人
• エピジェネティック制御を通した、リグニンおよび多糖分解モードのスイッチング

  公益財団法人京都大学教育研究振興財団・研究費助成

  From 11 Jul. 2018

  中沢威人
• 転写制御因子群の多重高発現による脱リグニン処理の効率化

  公益財団法人関西エネルギー・リサイクル科学研究振興財団・試験研究助成

  From 01 Apr. 2021, To 31 Mar. 2022

  中沢威人

Education

Teaching subject(s)

• From 01 Apr. 2024, To 31 Mar. 2025

  Laboratory Course in Forest Biochemistry

  BG15, Spring, Graduate School of Agriculture, 2.5
• From 01 Apr. 2024, To 31 Mar. 2025

  Laboratory Course in Forest Biochemistry

  BG15, Fall, Graduate School of Agriculture, 2.5
• From 01 Apr. 2024, To 31 Mar. 2025

  Laboratory Course in Biomaterials Chemistry

  6943, Fall, Faculty of Agriculture, 4
• From 01 Apr. 2024, To 31 Mar. 2025

  Laboratory Course in Applied Biomaterials Science

  6939, Spring, Faculty of Agriculture, 3
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar in Forest Biochemistry

  BF15, Fall, Graduate School of Agriculture, 2
• From 01 Apr. 2024, To 31 Mar. 2025

  Seminar in Forest Biochemistry

  BF15, Spring, Graduate School of Agriculture, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Laboratory Course in Applied Biomaterials Science

  6939, Spring, Faculty of Agriculture, 3
• From 01 Apr. 2023, To 31 Mar. 2024

  Laboratory Course in Forest Biochemistry 2

  BC69, Year-long, Graduate School of Agriculture, 5
• From 01 Apr. 2023, To 31 Mar. 2024

  Laboratory Course in Forest Biochemistry 1

  BC68, Year-long, Graduate School of Agriculture, 5
• From 01 Apr. 2023, To 31 Mar. 2024

  Laboratory Course in Biomaterials Chemistry

  6943, Fall, Faculty of Agriculture, 4
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar in Forest Biochemistry 2

  BB69, Year-long, Graduate School of Agriculture, 4
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar in Forest Biochemistry 1

  BB68, Year-long, Graduate School of Agriculture, 4
• From 01 Apr. 2022, To 31 Mar. 2023

  Fundamental Chemical Experiments

  N308, Spring, Institute for Liberal Arts and Sciences, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Laboratory Course in Biomaterials Chemistry

  6943, Fall, Faculty of Agriculture, 4
• From 01 Apr. 2022, To 31 Mar. 2023

  Laboratory Course in Forest Biochemistry 2

  BC69, Year-long, Graduate School of Agriculture, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Laboratory Course in Applied Biomaterials Science

  6939, Spring, Faculty of Agriculture, 3
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar in Forest Biochemistry 2

  BB69, Year-long, Graduate School of Agriculture, 4
• From 01 Apr. 2022, To 31 Mar. 2023

  Laboratory Course in Forest Biochemistry 1

  BC68, Year-long, Graduate School of Agriculture, 5
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar in Forest Biochemistry 1

  BB68, Year-long, Graduate School of Agriculture, 4
• From Apr. 2014, To Mar. 2015

  Basic and Application of Mushrooms

  Spring, 農学部
• From Apr. 2014, To Mar. 2015

  Laboratory Course in Forest and Biomaterials Science II

  Spring, 農学部
• From Apr. 2014, To Mar. 2015

  Laboratory Course in the Basic Forest and Biomaterials Chemistry

  Spring, 農学部
• From Apr. 2014, To Mar. 2015

  Laboratory Course in the Biomaterials Chemistry I

  Fall, 農学部
• From Apr. 2014, To Mar. 2015

  Seminar in Forest Biochemistry

  Year-long, 農学研究科
• From Apr. 2014, To Mar. 2015

  Experimentary Course in Forest Biochemistry

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Laboratory Course in the Biomaterials Chemistry I

  Fall, 農学部
• From Apr. 2015, To Mar. 2016

  Basic and Application of Mushrooms

  Spring, 農学部
• From Apr. 2015, To Mar. 2016

  Laboratory Course in the Basic Forest and Biomaterials Chemistry

  Spring, 農学部
• From Apr. 2015, To Mar. 2016

  Experimentary Course in Forest Biochemistry1

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Experimentary Course in Forest Biochemistry

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Seminar in Forest Biochemistry1

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Seminar in Forest Biochemistry

  Year-long, 農学研究科
• From Apr. 2015, To Mar. 2016

  Laboratory Course in Forest and Biomaterials Science II

  Spring, 農学部
• From Apr. 2016, To Mar. 2017

  Laboratory Course in the Biomaterials Chemistry I

  Fall, 農学部
• From Apr. 2016, To Mar. 2017

  Fundamental Chemical Experiments

  Fall, 全学共通科目
• From Apr. 2016, To Mar. 2017

  Laboratory Course in the Basic Forest and Biomaterials Chemistry

  Spring, 農学部
• From Apr. 2016, To Mar. 2017

  Experimentary Course in Forest Biochemistry

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Laboratory Course in Forest Biochemistry 1

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Laboratory Course in Forest Biochemistry 2

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Seminar in Forest Biochemistry1

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Seminar in Forest Biochemistry2

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Seminar in Forest Biochemistry

  Year-long, 農学研究科
• From Apr. 2016, To Mar. 2017

  Laboratory Course in Forest and Biomaterials Science II

  Spring, 農学部
• From Apr. 2017, To Mar. 2018

  Laboratory Course in the Biomaterials Chemistry I

  Fall, 農学部
• From Apr. 2017, To Mar. 2018

  Laboratory Course in the Basic Forest and Biomaterials Chemistry

  Spring, 農学部
• From Apr. 2017, To Mar. 2018

  Experimentary Course in Forest Biochemistry

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Laboratory Course in Forest Biochemistry 1

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Laboratory Course in Forest Biochemistry 2

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Seminar in Forest Biochemistry1

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Seminar in Forest Biochemistry2

  Year-long, 農学研究科
• From Apr. 2017, To Mar. 2018

  Seminar in Forest Biochemistry

  Year-long, 農学研究科
• From Apr. 2018, To Mar. 2019

  Laboratory Course in Biomaterials Chemistry

  Fall, 農学部
• From Apr. 2018, To Mar. 2019

  Laboratory Course in Forest Biochemistry 1

  Year-long, 農学研究科
• From Apr. 2018, To Mar. 2019

  Laboratory Course in Forest Biochemistry 2

  Year-long, 農学研究科
• From Apr. 2018, To Mar. 2019

  Seminar in Forest Biochemistry1

  Year-long, 農学研究科
• From Apr. 2018, To Mar. 2019

  Seminar in Forest Biochemistry2

  Year-long, 農学研究科
• From Apr. 2019, To Mar. 2020

  Laboratory Course in Biomaterials Chemistry

  Fall, 農学部
• From Apr. 2019, To Mar. 2020

  Fundamental Chemical Experiments

  Fall, 全学共通科目
• From Apr. 2019, To Mar. 2020

  Laboratory Course in Forest Biochemistry 1

  Year-long, 農学研究科
• From Apr. 2019, To Mar. 2020

  Laboratory Course in Forest Biochemistry 2

  Year-long, 農学研究科
• From Apr. 2019, To Mar. 2020

  Seminar in Forest Biochemistry1

  Year-long, 農学研究科
• From Apr. 2019, To Mar. 2020

  Seminar in Forest Biochemistry2

  Year-long, 農学研究科
• From Apr. 2020, To Mar. 2021

  Laboratory Course in Forest and Biomaterials Basic Science I

  Year-long, 農学部
• From Apr. 2020, To Mar. 2021

  Laboratory Course in Forest Biochemistry 1

  Year-long, 農学研究科
• From Apr. 2020, To Mar. 2021

  Laboratory Course in Forest Biochemistry 2

  Year-long, 農学研究科
• From Apr. 2020, To Mar. 2021

  Seminar in Forest Biochemistry 1

  Year-long, 農学研究科
• From Apr. 2020, To Mar. 2021

  Seminar in Forest Biochemistry2

  Year-long, 農学研究科
• From Apr. 2020, To Mar. 2021

  Laboratory Course in Applied Biomaterials Science

  Year-long, 農学部
• From Apr. 2021, To Mar. 2022

  Laboratory Course in Biomaterials Chemistry

  Fall, 農学部
• From Apr. 2021, To Mar. 2022

  Laboratory Course in Forest and Biomaterials Basic Science I

  Spring, 農学部
• From Apr. 2021, To Mar. 2022

  Laboratory Course in Forest Biochemistry 1

  Year-long, 農学研究科
• From Apr. 2021, To Mar. 2022

  Laboratory Course in Forest Biochemistry 2

  Year-long, 農学研究科
• From Apr. 2021, To Mar. 2022

  Seminar in Forest Biochemistry 1

  Year-long, 農学研究科
• From Apr. 2021, To Mar. 2022

  Seminar in Forest Biochemistry2

  Year-long, 農学研究科
• From Apr. 2021, To Mar. 2022

  Laboratory Course in Applied Biomaterials Science

  Spring, 農学部

Administration

School management (title, position)

• From 01 Apr. 2019, To 31 Mar. 2021

  北部構内実験排水系管理委員会　委員

Faculty management (title, position)

• From 01 Apr. 2022, To 31 Mar. 2025

  環境・安全・衛生委員会　委員

Academic, Social Contribution

Committee Memberships

• From Jul. 2023, To Jul. 2025

  評議員, リグニン学会
• From Apr. 2023

  Editorial Board, Journal of Wood Science
• From Jun. 2023, To Present

  Associate editor, Frontiers in Fungal Biology
• From Jun. 2019, To May 2023

  Review editor, Frontiers in Fungal Genetics
• From Nov. 2021, To Present

  執行役員（会計担当）, 糸状菌分子生物学研究会若手の会
• From Nov. 2019, To Present

  運営委員, 糸状菌分子生物学研究会若手の会
• From Apr. 2023, To Mar. 2025

  編集委員, 日本木材学会